LOCUS       DACEMK010000001        74952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEMK010000001 DACEMK010000000
VERSION     DACEMK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.001"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..184)
                     /locus_tag="IW715_000001"
     CDS             complement(<1..184)
                     /locus_tag="IW715_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020637.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPIL"
     gene            complement(481..2862)
                     /locus_tag="IW715_000002"
     CDS             complement(481..2862)
                     /locus_tag="IW715_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1020638.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2852..3499)
                     /gene="recU"
                     /locus_tag="IW715_000003"
     CDS             complement(2852..3499)
                     /gene="recU"
                     /locus_tag="IW715_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1020639.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3563..4102
                     /locus_tag="IW715_000004"
     CDS             3563..4102
                     /locus_tag="IW715_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1020640.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4192..4611
                     /gene="gpsB"
                     /locus_tag="IW715_000005"
     CDS             4192..4611
                     /gene="gpsB"
                     /locus_tag="IW715_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1020641.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4633..5000
                     /gene="rnpB"
                     /locus_tag="IW715_000006"
     ncRNA           4633..5000
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW715_000006"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5209..6375
                     /locus_tag="IW715_000007"
     CDS             5209..6375
                     /locus_tag="IW715_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1020642.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6421..7917
                     /locus_tag="IW715_000008"
     CDS             6421..7917
                     /locus_tag="IW715_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1020643.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7998..8681
                     /locus_tag="IW715_000009"
     CDS             7998..8681
                     /locus_tag="IW715_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1020644.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9326..10516
                     /locus_tag="IW715_000010"
     CDS             9326..10516
                     /locus_tag="IW715_000010"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1020645.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVGKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10618..11571
                     /locus_tag="IW715_000011"
     CDS             10618..11571
                     /locus_tag="IW715_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1020646.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11640..12989
                     /locus_tag="IW715_000012"
     CDS             11640..12989
                     /locus_tag="IW715_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1020647.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13172..13495)
                     /locus_tag="IW715_000013"
     CDS             complement(13172..13495)
                     /locus_tag="IW715_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020648.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13624..14787
                     /locus_tag="IW715_000014"
     CDS             13624..14787
                     /locus_tag="IW715_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020649.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14857..16206
                     /locus_tag="IW715_000015"
     CDS             14857..16206
                     /locus_tag="IW715_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1020650.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16251..16682)
                     /locus_tag="IW715_000016"
     CDS             complement(16251..16682)
                     /locus_tag="IW715_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1020651.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17070..19712
                     /gene="alaS"
                     /locus_tag="IW715_000017"
     CDS             17070..19712
                     /gene="alaS"
                     /locus_tag="IW715_000017"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1020652.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19912..20508
                     /locus_tag="IW715_000018"
     CDS             19912..20508
                     /locus_tag="IW715_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1020653.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20630..21778
                     /locus_tag="IW715_000019"
     CDS             20630..21778
                     /locus_tag="IW715_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1020654.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21816..22229)
                     /locus_tag="IW715_000020"
     CDS             complement(21816..22229)
                     /locus_tag="IW715_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1020655.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22308..22739
                     /locus_tag="IW715_000021"
     CDS             22308..22739
                     /locus_tag="IW715_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1020656.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22838..23071)
                     /locus_tag="IW715_000022"
     CDS             complement(22838..23071)
                     /locus_tag="IW715_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1020657.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23254..24924
                     /locus_tag="IW715_000023"
     CDS             23254..24924
                     /locus_tag="IW715_000023"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1020658.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25071..25457
                     /locus_tag="IW715_000024"
     CDS             25071..25457
                     /locus_tag="IW715_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020659.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25444..26142
                     /locus_tag="IW715_000025"
     CDS             25444..26142
                     /locus_tag="IW715_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1020660.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26147..26611
                     /locus_tag="IW715_000026"
     CDS             26147..26611
                     /locus_tag="IW715_000026"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1020661.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26608..27519
                     /locus_tag="IW715_000027"
     CDS             26608..27519
                     /locus_tag="IW715_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1020662.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27754..28293
                     /gene="pyrR"
                     /locus_tag="IW715_000028"
     CDS             27754..28293
                     /gene="pyrR"
                     /locus_tag="IW715_000028"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1020663.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28307..29590
                     /locus_tag="IW715_000029"
     CDS             28307..29590
                     /locus_tag="IW715_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1020664.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29603..30529
                     /locus_tag="IW715_000030"
     CDS             29603..30529
                     /locus_tag="IW715_000030"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1020665.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30548..31828
                     /locus_tag="IW715_000031"
     CDS             30548..31828
                     /locus_tag="IW715_000031"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1020666.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31833..32909
                     /gene="carA"
                     /locus_tag="IW715_000032"
     CDS             31833..32909
                     /gene="carA"
                     /locus_tag="IW715_000032"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1020667.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32914..36099
                     /gene="carB"
                     /locus_tag="IW715_000033"
     CDS             32914..36099
                     /gene="carB"
                     /locus_tag="IW715_000033"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1020668.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36153..36929
                     /locus_tag="IW715_000034"
     CDS             36153..36929
                     /locus_tag="IW715_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1020669.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36926..37858
                     /locus_tag="IW715_000035"
     CDS             36926..37858
                     /locus_tag="IW715_000035"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1020670.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37855..38571
                     /gene="pyrF"
                     /locus_tag="IW715_000036"
     CDS             37855..38571
                     /gene="pyrF"
                     /locus_tag="IW715_000036"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1020671.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38578..39207
                     /locus_tag="IW715_000037"
     CDS             38578..39207
                     /locus_tag="IW715_000037"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1020672.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39195..39509
                     /locus_tag="IW715_000038"
     CDS             39195..39509
                     /locus_tag="IW715_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020673.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39547..40188
                     /locus_tag="IW715_000039"
     CDS             39547..40188
                     /locus_tag="IW715_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1020674.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40219..40923
                     /locus_tag="IW715_000040"
     CDS             40219..40923
                     /locus_tag="IW715_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1020675.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40920..41822
                     /locus_tag="IW715_000041"
     CDS             40920..41822
                     /locus_tag="IW715_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1020676.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41980..43686)
                     /locus_tag="IW715_000042"
     CDS             complement(41980..43686)
                     /locus_tag="IW715_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1020677.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            43876..44499
                     /locus_tag="IW715_000043"
     CDS             43876..44499
                     /locus_tag="IW715_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020678.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            44949..45998
                     /locus_tag="IW715_000044"
     CDS             44949..45998
                     /locus_tag="IW715_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1020679.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            45995..46876
                     /locus_tag="IW715_000045"
     CDS             45995..46876
                     /locus_tag="IW715_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1020680.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            46879..47649
                     /locus_tag="IW715_000046"
     CDS             46879..47649
                     /locus_tag="IW715_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1020681.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            47928..48782
                     /gene="aroE"
                     /locus_tag="IW715_000047"
     CDS             47928..48782
                     /gene="aroE"
                     /locus_tag="IW715_000047"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1020682.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            48960..50123
                     /locus_tag="IW715_000048"
     CDS             48960..50123
                     /locus_tag="IW715_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1020683.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            50240..50650
                     /locus_tag="IW715_000049"
     CDS             50240..50650
                     /locus_tag="IW715_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1020684.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            50824..53052
                     /gene="topB"
                     /locus_tag="IW715_000050"
     CDS             50824..53052
                     /gene="topB"
                     /locus_tag="IW715_000050"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1020685.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            53190..53507
                     /locus_tag="IW715_000051"
     CDS             53190..53507
                     /locus_tag="IW715_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020686.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            53667..53903
                     /locus_tag="IW715_000052"
     CDS             53667..53903
                     /locus_tag="IW715_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1020687.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            53900..54298
                     /locus_tag="IW715_000053"
     CDS             53900..54298
                     /locus_tag="IW715_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1020688.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(54965..55909)
                     /locus_tag="IW715_000054"
     CDS             complement(54965..55909)
                     /locus_tag="IW715_000054"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1020689.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(56205..57149)
                     /locus_tag="IW715_000055"
     CDS             complement(56205..57149)
                     /locus_tag="IW715_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1020690.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            57370..58584
                     /locus_tag="IW715_000056"
     CDS             57370..58584
                     /locus_tag="IW715_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1020691.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            58606..60621
                     /locus_tag="IW715_000057"
     CDS             58606..60621
                     /locus_tag="IW715_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1020692.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(60704..62077)
                     /locus_tag="IW715_000058"
     CDS             complement(60704..62077)
                     /locus_tag="IW715_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1020693.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(62103..62387)
                     /locus_tag="IW715_000059"
     CDS             complement(62103..62387)
                     /locus_tag="IW715_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1020694.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(62389..64404)
                     /locus_tag="IW715_000060"
     CDS             complement(62389..64404)
                     /locus_tag="IW715_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1020695.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(64583..64888)
                     /locus_tag="IW715_000061"
     CDS             complement(64583..64888)
                     /locus_tag="IW715_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1020696.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(64977..66008)
                     /locus_tag="IW715_000062"
     CDS             complement(64977..66008)
                     /locus_tag="IW715_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1020697.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            66215..67090
                     /locus_tag="IW715_000063"
     CDS             66215..67090
                     /locus_tag="IW715_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1020698.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(67242..68084)
                     /locus_tag="IW715_000064"
     CDS             complement(67242..68084)
                     /locus_tag="IW715_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1020699.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(68176..70053)
                     /locus_tag="IW715_000065"
     CDS             complement(68176..70053)
                     /locus_tag="IW715_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1020700.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(70050..71471)
                     /locus_tag="IW715_000066"
     CDS             complement(70050..71471)
                     /locus_tag="IW715_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1020701.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(71474..74863)
                     /locus_tag="IW715_000067"
     CDS             complement(71474..74863)
                     /locus_tag="IW715_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1020702.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
BASE COUNT        23620 a        13370 c        15502 g        22460 t
ORIGIN      
        1 caaggatagg agcaattaaa gtaaatttat ttaataataa acaaaatttt tcagcttttt
       61 tattcttgga ataacgaggt attgttttcg ataaaaaaag atggtgcaga caaattatga
      121 taattaagca tactatcaga taactcaaaa tgaagccttc tttcaaaaaa ttattcaaaa
      181 ccatatattg tattttttat ttgataataa caatgggatt attaagaagt ggcactaaat
      241 aaaagactag tgtgtattta gccgatttaa ttataaacaa gtaaatggat aaagtatatg
      301 taatgataaa agaaaaatgg attttctaat ttttaagcta aatgttagat cacacatgta
      361 tatgctggat agtcttataa aaaatcatgt aatacagctt ggtacatgat ttttatttgt
      421 agctgttttt tacatagaca caaaaaagaa accacgcttg tagacgtggt ttctttttta
      481 ctatctgttt ccagaagctg aggtgctgct ggatggtgta tggtcatttg cagcatccgc
      541 tccgctagtt gctggctcac tggaagaagc aggaggagca gatgaagacg gtgtcgagct
      601 ttcttcggat gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt
      661 cgtgctttct tttgaggact cagcagttga ttcgctttga ctagtagagc tagagctttg
      721 agtcgttgtc tctgttgttg agcttccagg agtctggaca tagctggaag atggaatagt
      781 tgttgatggt gtaattgcat tcgatctagc tgtatattga tctttgtagt acagttcacc
      841 gcccacacga attaatccgc ttggcatctc ccagtctgta tttgtcacgt tagctgaaac
      901 atactgcata agctcgcgat aaacatcaga ggctacgtgt gacgattctg aagtgacagg
      961 tgtcattttt ttattgtaac ctgtccacac ggaaatcgaa taatttggcg tatagcccgc
     1021 aaataggata tctggataca ccccagaaga aataccaagt tttgcatact cgtcatctgt
     1081 gtaattggat gttccggtct taccggcttg atataaccca gcgatttgcg cattcgtacc
     1141 agttccttca gtgatcgtat cttttaaaat gtctgtgatc atataagctg tttctggaga
     1201 catggctgtt tttccatctg gctcataagt ttcttctgtt ccgtcttgga agacgatttt
     1261 attaacatat tgaggtttgt aataagtccc tccattggcg aaagccgcat aggctgctgc
     1321 catttttaag gaagaagctc cgtattttgt cccatcttgt tcttccgtat tactagagat
     1381 cgcattcgat tgatggatcg tactgtattc gatcccaaga ttttttaaga aagaagccac
     1441 tttatctgaa ccgacttcat tgaataattt gactgctgga acattacggg ataagtaaag
     1501 tgcttggcgt aaagtaatgg tgcccatata ttgattatcc cagtttccta caggagtcga
     1561 agtcccttca tagttatacg gtgcgtcagt aatagttttt ccagtagaat atttcaaata
     1621 ttcaaaagct ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt
     1681 attcactgct aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg
     1741 ggcttttact ttcccggtat tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc
     1801 tgggtaagag acatactgat ccgtgttgac gatatcatat agtttctttt gggcgtccag
     1861 atccaaattc gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg
     1921 tacttcattg atgacttctt tgacataatt atcgacgatc ttagtattgt catcggattg
     1981 agttagttct tgcagaccat ccgttaccgg tacattaaca gcttgatcat attctgtttg
     2041 agagattttt tcgttttgaa gcatcgtata caaaacagta tctcgtcttt tcttcgcctg
     2101 atctggataa acataaggat cataggcgga aggggcttga ggcatcccag caagcaatgc
     2161 tgtttgcggt aaagaaagtt ctgataattt cttaccaaag tacatctcag aagctgtctc
     2221 catcccgtac aagccgtttg acatatacac tttatttacg tagtaggtca agatctcttg
     2281 tttcgatttt ttttgttcca atcttacagc catccacgct tcttgggcct tgcgtttcag
     2341 agtctgatcc tcggcactgg tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt
     2401 acttccgcct tgcagtccgc cagaggtaaa gttagagagg gcagaaccga tgatccgaat
     2461 agggtcaacc ccgatgtgtt tataaaaacg gcgatcttct accgacacga tcgcatcttc
     2521 taatgttttc ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc
     2581 gaatagttcg ccatcttgtg tatatagttt ggaagatacg gtagcgtcta attttttatc
     2641 cgtcaattcg ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag
     2701 gatacagaaa aatgagagta ttcctaaaag gatcttgata aataatcctt ttttatgtgt
     2761 tccagaggac tttccagaac ctttgccaga attcttcttc acactctttt taggagtagg
     2821 ctgtttctgt cgtctagagc ttctagtttg ttcatttgcc attcttgctt tctccttcaa
     2881 tagataatgt tgataaatag aagtcaaccg cgtccaaata aggaatccgc ggagaaatac
     2941 cagttttgat ctctattcct agttcttcga tcaaagataa aggtagtgat ttgcgtcctt
     3001 tttccttttg ggcgtcccag taagcaatca aatgaacact attcaaataa aaacagcgct
     3061 tcaaagacga aaaccataac aagacaaagc agaccccatt ttgttcgagg catgctttca
     3121 tatgttcgat ttgatgggca tggaagtttt taaagggaaa agatgttttg ttcttggttt
     3181 cttttgcttc aaagtccaag taaaagcctc gatagacgcc attatagtct gtagttgagg
     3241 cttgggtaaa gtaggcttcc ttgatcacag cagcacttct cttaggatag tctactttca
     3301 cgatttggat aggggtaggc tttttatgga taaccgccaa cccgcgggaa agataatatt
     3361 tattactttc attgattgct tcttcaaagc gcatcccccg attgccgaat tcagtgatct
     3421 gatttctttt ttcttgaatt ttttcgacgg gagaggcatt cttataataa ggttggccat
     3481 ttggatagcg caataccatc tttaatcaca ctcctaggcc acattatagc aaaaaagcgg
     3541 atagaaagaa aaggaatctg agatggataa gtcaaaagta atctatgtgt ctgggtatcg
     3601 aagtttcgaa ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt
     3661 tttaaaaaaa gaactccatc agttagcaga agaagggctg gaatgggtac ttgtcagtgg
     3721 gaatttgggc gtcgaactat gggctgtgca aatagtagcg gaattgaaag aagaatatcc
     3781 ggaactatct cttggtattt tgtatccttt ttctgatttt ggtaataact ggaatgaagg
     3841 gaaccaagaa aaaaaacaat tagcagaaca attagcagat tatgtcgaag ctgttagcca
     3901 tcaaccctac caatcaccta gtcagctaaa gaatcacact cgttttatac ttgagcatac
     3961 agtgggctgt ctgctgatct atgatgaaga attccctgga aaaacgaagt atttttttga
     4021 agatgcacag aattaccaag cagatcatcc ctacgaaatt cggctgatca gtatggacga
     4081 tcttcaaaat tttagtgaat aatcgatatt gtgtttgatt tatcaacttt tttcctatac
     4141 aatgaaaaag gtagaaatgt tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat
     4201 ttggtttata gtcctaaaga cattttacaa caagaattca aaacaaaaat gcgcggctat
     4261 gatcccgttg aagttgatga attcttagac aatatcatca aagattatga aacttacagc
     4321 aaagaactcc ttgctttaca agaagaaaac gatcgattga gtgcaaaagt agctcaatta
     4381 tctaaaactc aaggagcagc tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca
     4441 gcagtgacca attttgatat cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt
     4501 aaaaagcttg atcaacaagc aagtgcagtc aaaccagcac aacctaatcc aaataactac
     4561 acaaatgcag acacaagctt agatgataat gaaaaaacac gtcaatttta atttttgatt
     4621 aatgaatttg cggttttcgg gtaatcgcgg tctgacttga tcaggctgag gaaagtccat
     4681 gctcgcacag gctgtgatgc ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac
     4741 tctttggagt aacggcagga aaacaagcta aggcttcggc tatgcttaag tatccttgaa
     4801 agtgccacag tgacgaagcg gctggggaaa cccatccggt ggaacgcggt aaacccctcg
     4861 agcgagcaac ccaaacaatg gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa
     4921 ggcagtataa ctgcagatag atggttaccg ctttgttttt tctccctgga aaagcaaagt
     4981 acagaacatg gcttatagaa aaccgcaaac atcagggaat taggtcgtga caaaagtaat
     5041 gcgttataag aaacaagaaa aacatttgaa agctattttt caatacttca gatgaatcgt
     5101 tcttattaca aaacaaactt ttgtatcacc ggttattcgg aatttgagta aaagtgtggg
     5161 gtaggactga tgtcccacct ccttttttta taaataagga gattgtgtat ggaaaaaaac
     5221 aggaagttca acctcgtcgc aactgcggca agcgggctag aagcactggt tggtaaagaa
     5281 ttaagagatc ttggaatcga atgccaagtt gagaacggac gtgcacgttt tgaaggaaca
     5341 atggaaacga tcgctacagc taatttatgg ttacgtacag cagatcgagt aaaaatcgtg
     5401 gttggggaat tcgatgcgta tacgtttgat gaattgtttg aaaacgtaaa agcattgcct
     5461 tgggaagatt ttcttccact agatgcagct tttcctgtag ctggaaaatc gatcaagtcg
     5521 aaactatata gcacacctga ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc
     5581 cgtacctatt atcaccgtcc agcttctgta ccactgacgg aaactggcgc acattttcag
     5641 ttggaagtag ctttgctgaa agaccatgtg atggtaactc tggatacgac aggaccaagt
     5701 ttattcaaac gtggttatcg cttagaaaaa ggtggagcac cattgaaaga aaacatggca
     5761 gcagcactta ttatgttgac aaattggcga aaagaccgtc cattttatga tcctgtatgt
     5821 ggttcaggta cgctttgtat tgaagcagcc ttaataggcc ataatatcgc accaggattc
     5881 aatcgtgagt ttgcttgcga atcttgggat tggttcagtc aagaaatcat ggaaaatgtt
     5941 cgatcagcag ccgaagaaaa agcagattat gatattgaat tagatattac tggttcagat
     6001 atcaatggac ggatgattga aattgccaaa gccaatgcag aagaaatcgg attaggctct
     6061 tcgattacct ttaagcaaca agcagtcaaa gacttcaaaa cggataaaga atacggggtc
     6121 atcgtggcta atccgcctta cggagaacgt ctaggagaag aagaaactgt tcggaaatta
     6181 tataaagaaa tgggcgatgt attccgtcca ttaaaaacat ggagtaaata tatcttgact
     6241 agcgacttag cgtttgagga attttacgga caaaaagcaa ccaaaaaaag aaaattgtac
     6301 aatggtgcat tgcgtacgga tctattccag tattggggaa ctcgtccgcc tagaaaaaaa
     6361 gagaaagacg aataatctta ctaattgatt cattcaattg gatgtttgga ggaataggga
     6421 atgaaagaaa aagagttttt acaagaagta aaagaaatca atcttctaca acaagcttta
     6481 ggaatcctcg attgggatac acaaacaggt atgcctgaaa aagctagtgc ataccgaagt
     6541 gaagtagaca gctatcttta cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa
     6601 gaagccattc gctatttttc tgaacatccg gaagaattgt cggaagttgg aactgccgtt
     6661 tttgccaaag taaaagaaga gtatgatttg gaacatcgcg taccggaaga tcttatgaga
     6721 aaatatacag ctgccgtttc ttcagcacac ggacaatggc aaaaagctag agaaacacaa
     6781 caattttctg attttcaaca cgcattgaat caaaatattg aattaacaaa acagttgata
     6841 ccttactgga gaaaagatga aaagacgaat tatgatgttt tgctgaatca atacgaaccc
     6901 gggatgactg tcgaaatcct tgataaggta tttgatcagc ttaaagaagg aattctttcg
     6961 atccgccgta cgctccaaga aaaagggact gaacctgaaa cagacttttt aaatcggaga
     7021 atgacgaaaa aacaacagaa gagatttgta gtgaaagtaa tcgaacaact tggttatgat
     7081 tttagtcgtg gtcgtttaga tgacactgtc cacccattta tgactggaat taatccgaat
     7141 gatgcacgaa tcacgacacg atggaacgaa acagatttca aaatggctgt ttttggagtg
     7201 atccacgagg ctggtcatgg tatgtatgaa caagacatcg atgataaata tgcgtataca
     7261 ccggtttatg aaggtacatc aatgggtatc catgaatcac aatcgttgtt taacgagatc
     7321 attattggaa gtagtcgcag cttctggaaa aagcaatatc ctttctttca agaatgtgca
     7381 gaaggaacat tcgatgatat tccttttgac aaattttatc gttcattgaa ccatacaaaa
     7441 tcaagtttgg ttcgtatcga ggcagacagt ttgacttacc ctctccatat catcattcgt
     7501 tatgaaatcg aaaaaatgat tttcaatgaa ggggtagatg ttgctgaact acctgaaata
     7561 tggaatcaaa aatatgaaga gtatctggga atacgaccaa caaatgatgt ggaaggcatc
     7621 ctacaagacg ttcattggtc aggtgcaagc tttggttatt ttccctctta tgcattaggg
     7681 tacatgtatg cggcacaatt acttcattct ttgcagcaag aaatagatgt ggaaaatgtt
     7741 ctagcgtcag acgattattc tccaatcaaa aactggatgt cagagcatat ccatcaatat
     7801 ggtgcatcta gaaaaccaaa tcaattaatc atggatgcaa cacatgaacc gcttaatccg
     7861 caatacctga tcgactatat gaaagacctg tattttaatg tatatcaggt aaaataaaat
     7921 acaatttaca aagatagctg aaattcatct aatctataga tgttttcagc tatttactta
     7981 agaaagaagt ggaacgaatg gataaattac aagtgatcgc tgattatagt atggaccaat
     8041 tagcgcatga tcaaacagga catgggagcg atcataccaa aagagttgtc aagttagctg
     8101 aaaaaatttt ggagacggaa ccgcaggcag accgctttgt gacgcttgca gcagcttacc
     8161 tacatgatac gattgatgat aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc
     8221 gtgtattcct tcgctctttg ccaatagtag aggaacagat ctcgatgatt ttttccatta
     8281 tcgaaaatat gtccttttct aaaaatctct ctgaagcggc cgaactatct ctggaaggta
     8341 agatcgttca agacgctgat cgtatcgaag cattaggagc aatcggcatt ttgcgtactg
     8401 cttatttcgg cggtggacat gggcacccga tttttgatcc tgaactgtat ccgcaaacat
     8461 tcaaggacaa agaaaattac cgaaaaggca cgacagtgat caatcatttt tatgaaaaac
     8521 tgttcttgct gtctgataaa atgaatactg attacggata tgaagaagcc aaacgaagag
     8581 aaaccttcat gcgtgaattc ttagaagaat tttttctgga atggtctatt gacgatgaaa
     8641 attttactcc agacaattgg atcaaaaata acaaaaaatg atggaggttg tgacagaagt
     8701 ggtcagctcc aagatatttc ggatcatttt ccgtaaaagt tacttctgga acactgttta
     8761 ccaagaggtc gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt caaaaaatag
     8821 cttctcgtgt ttgatgaata ttgtaacttg tgattcggaa ttggcaggag aacaccgttt
     8881 attcggaaga ataggactgt gacaagactt ttgtcacagt ccttttttcg aattcaacaa
     8941 gtaaaacttt taagaaatat tcagagagta aaattaaaaa ttgctgataa acgaagatgg
     9001 aaattttatg tatttttatt ggtctatgca ataaataaat taaatattca gaatacaatt
     9061 gattttaacg aaagggcttc tatcaatggt tgtgttgtat ttatctgccc aggtagagta
     9121 aaatagatca ttatctaaaa ggtaattgtt catactatat aagtcatttg aaagtaaaaa
     9181 gatactgtaa ataaaaaatg tggttaaata attctcataa tcagtgtaag cgttatcaaa
     9241 aatatagcat acttgctttg tagatgaatg acacgatata tgtttggtat gttctgcaag
     9301 acagatgaat atggaggtgg agacaatgtc ttccaaagaa ctaacaatgt atttagagaa
     9361 agaattggat caactgaaag aaaaggggtt atacaatacg atcgacatac tagaaagtga
     9421 aaatggtgct tgtatcatcg tagacggaaa aaagatgatc aatctagcat ccaacaatta
     9481 tcttggattt gccaatcgcg aagaattgaa aaaagcatgt attgaagcaa ccgaaactta
     9541 tggtgtagga gcaggagcag tccgaacgat caatggttcg ctgaaaatcc atcagcaatt
     9601 agaagaaaaa atcgcagaat tcaaagggac agaagcagca atcgcctttc aatctggatt
     9661 caactgcaat atgggcgcta tctctgcagt aatgacaaaa gaagatgcta tcttatcgga
     9721 tgaattaaac catgcatcta ttattgacgg ttgccgctta tccggagcaa aaatcatccg
     9781 aattaaacat caagatatga aagaccttga gaaaaaagca aaggaagcga tagaaagtaa
     9841 gaaatataaa aaaatcatgt atatcacaga tggtgttttc tccatggatg gagacattgc
     9901 acgattgccg gaaattattc cgattgttgg aaaatatgga ctgatcacct acgtcgatga
     9961 tgcgcacggc tcaggcgtaa ctggaaaagg tgcaggaacc gtcaagcatt ttggattaag
    10021 cgacaaaatc gatatgcaaa tgggcacgtt atcgaaagct ataggtgttg ttgggggtta
    10081 tgtagctgga tcgaaaacac taatcgactg gttgaaagct agaagtcggc cattcctatt
    10141 ttctacttcg ctaactccag gtgcagctgc agctgcattg gcttcgatta ctttgatgca
    10201 agaacatccg gaacttgtcg aaaaagtttg ggaaaatgct aattatttta aagaagaatt
    10261 gaaaaaagta ggctataaca tcggtatgtc tgaaacacct attactccag tcattttggg
    10321 agacgaaaaa gtgactcaga ctttctctaa gaagctgatc gaacatggga tttatgctaa
    10381 accaatcgtt tatccaactg tgcctcttgg gacaggcagg atcagaaata tgccgactgc
    10441 ggaacatacg agggaaatgc ttgatgaagc agtagcagtt tatcaaaaaa taggaaaaga
    10501 aatggaaatt atttaactaa atttagtttt ttcgtctatt taaaattaga gaacatgttc
    10561 acgcatatat tgttaatcat ctgaagatag tagtaaaaaa gatcagggag gatcatcatg
    10621 aaaaaagtat tggtgacagg atgtttagga caaattggtt cagagttgat cacacggcta
    10681 cgtcaagaac taggcagtga aaatgtcatt gcgacagata tacgcatgcc tgaagtcatt
    10741 gagaaaaacg atatctttga gatcctagat gtaatggatt acgaaaaaat gcgcagcata
    10801 gcagaacaat atcaagtgga cacattgatt catttggctg ctcttttgtc agctgttgca
    10861 gaagcaaaac cacaattagc ctggaatttg aacgtgactg ggctggtcaa tgcattggaa
    10921 gtcgctagag aattccagct gaaatttttt acgccaagtt caatcggcgc gtttggtcca
    10981 aatactccaa aagatcatac cccacaagat actcttcagc gtcctacaac catgtatggg
    11041 gtaacgaagg tagctggaga actactatgt gattattatt atgtgaaata tggcgtagat
    11101 actcgtggtg tccgtttccc tggattgatt tcttatgcaa ccttgccagg aggcggtacg
    11161 acagactacg cagtcgatat ttattattca gcaatcaaag aaggaaagta cactagtttc
    11221 attgatcaag gtacatcaat ggatatgatg tatatgccag atgcgatcga tgccattata
    11281 aaactcatga atgcagatcc tgatcgattg gttcatcgaa atgccttcaa tatatcggcg
    11341 atgtcttttg acccggaaga gatcaaagca tctattcaaa aaatcatacc agattttgaa
    11401 atggattatg atgtggatcc tgtacgtcag tcaatagcag aatcttggcc aaattcacta
    11461 gatgtttctt gtgcgcaaga ggaatggggc ttttcgccga aatacgactt agatgtgatg
    11521 acaaaggata tgttggaaaa actgaaagaa aaattggtag agacggtata aaacaagaac
    11581 cacatccaaa aagtttttta tacttttggg attggctatg gtatgaaagg ggacaaggga
    11641 tggaagaata tcaagccacg ccagtaaaag aagtaaaaat agaaaatgaa ttaaaacgta
    11701 cgatgggatt ttttactgct ttatcgactg ttatgggcac agtgatcggt gcaggggtat
    11761 tttttaaagc cgcaagcgta gcagaagtta caggatccgc cagtttgcat atgttttctt
    11821 ggtttctcgg tggtatgatt tctgtctgtg caggactaac aggagctgaa ctggcagctg
    11881 ctattccaga aacaggaggg atgattaaat atatcgaacg gatttacgga aatacagcag
    11941 cttttttatt gggctgggcg caagtcgtga tttattttcc agcaaatgta gcagccttgt
    12001 cgatcatttt cggtacacag ttcgtcaatc tatttggtct ttcccagtca atgattgttc
    12061 ccgtagctgt tacagctgct gtatcgatcc tgttgatcaa ttttttaggt tctaaagctg
    12121 gaggggcttt tcagtcgatt acgcttgtct gcaagcttat tcctttgttt gtgatcgtca
    12181 ttttcggttt gttccgacaa gaaggagtag acttccaact gtttccgatc caagcaggag
    12241 aaaatctttc atttttctca gccttgggag cgggcttgct agcaacgatg ttcgcatacg
    12301 acggttggat acacgtagga aatatttcgg gagaattgaa aaagccggcg aaagatttgc
    12361 caaaagcaat ttctttagga atcatcggaa tcatgatcgt ttatttatta gtaaatgcgg
    12421 tgttcttaag aaccgcatct atcgacggag tcgcaggaaa cagcaatgcg gcaagtgacg
    12481 ttgcaaaaat gatttttggc ggcttcggtg gaagactggt cacagttggt atcttgattt
    12541 ctgtttatgg aaccatcaat ggctataccc ttactggaat gcgtctccct tatgttatgg
    12601 caaaagaaaa caacctgcct tttagcaagc tctttgctaa acttcatgat aaaactaaag
    12661 ttcctgtagc cgcaggtatc ttagagctag tcatcgctat tggcatgatg atgatcggtg
    12721 gttttgatac actaacggat atgctgatct ttgtcatttg gattttttat acgatggtct
    12781 ttgttggtgt cattcttctt cgtaaaaaag aacctgattt gtttcgacca tacaaagttc
    12841 caatgtatcc gtttattcca ctagtagcaa tcattggagg gacttttatc gtatcaagca
    12901 cgcttatcac tcagaccttt ttagcttcga tggggatagt attgacactt gctggtatcc
    12961 cgatttatct ctatttaaaa cgtagatagc taaaacattc gaattttatt agaactgcac
    13021 ttctctgcca aacaaaatat tttatttaaa tgaggagtac gtggaagaaa tgtcaaaagt
    13081 actcgttaat tttgatcacg taaatgtcgt tttgtctacg atgaaatagc aaagtaaaag
    13141 acagaaatcg ggctcaaccg atttctgtct tttactttga tttgcgtaac tgaactattc
    13201 ggcgtacgcc gcgataaata tttaataaaa ccaagcaacc agaaatgaaa atgggaagcc
    13261 aaatcagata gacagggtag ccaagcactt tagacaaagc atgtggagtc attttatcca
    13321 tttcaagaag tgcttgctgc caagtctgaa gattgaagac tagcatgaaa cccgaaataa
    13381 tcaggagaat aaatcctaaa taatgaattg tagttctttt tcttacataa gcgatcgtag
    13441 cataaaggaa cagtaaaaaa ataggtacgc caaaaacaaa gtacatattc agcatctcga
    13501 cacccccttt gtgtttatct tactttactt tgaaagaaat gagaaataat agcctcgtta
    13561 cttgcttgaa acagcaaagg actgctaaga taagagagat acaaagaatc aaggaggagt
    13621 catatggcaa gttatatcga accgattcgt tttgcgctga ttgtttttcc atttttagcc
    13681 ttagcgatct catctgtttt ttttatttat gaatatcgaa agtatggaac atttctagta
    13741 acaagggcag ttattttgta ttctttcgtt ttttatttat tgtgtgcgta ttttttggtg
    13801 atcttaccac ttccggcaaa ggagacggtt gcccaaatga cagggccaaa gatggagctg
    13861 catttatttg cttcatggca acatttcaga gacaacacgg tattggtctt gtctgaacct
    13921 agtacctacc tgccggcaat gaagcagagt gtatttttgg aaccagcttt taatgtttta
    13981 ttactcatac catttggcat ttatttaaga tattatttcc ggctttcttt tatgaaaacg
    14041 atgattgcta gtttctgcct ttctctattt tttgaattga cgcagctaag tggactttat
    14101 tttatctacc caagaccata ccgcttattt gatgtgaatg atttagtgac aaatacattg
    14161 ggtggaatca ttggttttgt cgttacgccg ttatttacat ttatgcttcc tacaagaaga
    14221 cgaatggatg aactttctta tgaaaaagga aagactgtct cattgactcg acgcttggtc
    14281 gcttatttga tcgactggac atttttatct cttattaatt tggtagcggc gattttcttt
    14341 aaaattgcgt tcgattcagg ggcagttact tctcatatct ggtggctttt attagaagta
    14401 ggggtatact ttatgattgt accgtttata accaatggat ggacacttgg caaaaaaatt
    14461 gtgcgtatcc gtttggtgga agaagaccgc gaacgaattt ctttaaaagc attgatgatc
    14521 cgctacgggt atctgtactt tcttttttat ggtttaagct ggttggggca gttgcttcag
    14581 caaatcatga attcgacaac gaatacggtg ttgcagatga gtgcagtatt catttctctc
    14641 ggtgtcttgt tactacaagg tatttttatt ttaaatatct tgctatcatt tttgagaaaa
    14701 aaacggattc tgttttatga aaaagctagt cacacatata ctgttagcac gattggccaa
    14761 acaaaggatg atgattctcg aaattagctg agcaaaaacg ctttgtctgt gttataatgc
    14821 caaagtcgtg ataatttaaa gagaagaggt gcacgaatga ataattttga tatattccgc
    14881 ttcaaaccgt ttatcaatga agcgttaacc gcaaagggct tcaaacagcc cacagaggtc
    14941 caggaacgtt tgattccttt aattgcaaaa ggaaaaaata tcattgggca atctcaaact
    15001 ggtacaggaa aaacacatac gtttttgttg cctttgtttg aaaaaatcaa tgcagaaaaa
    15061 gaggaagtac aagttgttat tacagctcca agtagggaac tagctgcaca gatctatgaa
    15121 gcagctacac aaatcgctaa attttctaca ccggagatcc gtgtctcgaa ttttgtcggc
    15181 ggtacagaca aacagcgtca attaaatcgt ctgcatcacc aacagccaca ggttgtagta
    15241 ggcacgcctg gtagaatatt agacatgatg aatgaacaag cgctgaagat tcacaccgca
    15301 tttgcatttg ttgtcgatga agcggatatg acattggata tgggtttttt aaatgaagta
    15361 gaccaaattg caagtcgatt accagaaaag ctgcagtttt tagttttttc tgccactatt
    15421 ccagaaaaac ttcgtccatt cttacgaaaa tacatggaaa atccaatcat tgaagaaatc
    15481 aaaccaaaag cagttatttc tgaagacatt gaaaattggc tgatttcaac aaaaggtaaa
    15541 gatgagaatc aaatgattta tcaactgctg acaattggtc atccttattt agcaatcgta
    15601 tttgccaata caaaacagca tgtggatgaa attgccgatt acttgaaaaa ccaaggcttg
    15661 aaagtagcaa agattcatgg agacatcacc ccaagagaga gaaaacgagt gatgcgacaa
    15721 gtacagaatc tggagtatca atatgttgta gcgactgatt tagctgctag agggatcgat
    15781 atcgaaggtg tctctcatgt tattaatgca gaaattccag aagatctaga cttctttatc
    15841 catcgtgtcg gacgtactgg acgtaatggc ttaaaaggaa cggccattac gttatattcg
    15901 ccaaatgatg agcaagccat tgatgcgata gaaaaattgg gcgtctcatt tgaaccaaaa
    15961 gaaatcaaaa acggggaaat cgtcaaaaca tacgatcgta atagacgtgc caaacgagaa
    16021 aaatcaaaag atgtattaga tcccacgctg attggtcttg tgaagaagaa aaagaaaaag
    16081 atcaagcctg gttataagaa aaaaatcgat tgggcaattg cagaaaaaaa taaaaaagaa
    16141 cgcaaaatcg aacgcagaca gcaaagccgt actgctcgta aaaataagaa aaattcacat
    16201 aaataaaaaa caagaggttg tgacaaaagg cgtgtcacaa tctcttgttt ttaagctaga
    16261 ctttttttca ttccgataca aagtacttca tcttcaataa aaggttctgt gcaagtatga
    16321 tacccttgtt tttcataaaa atctacagcc gtcatttcag ctactacata actagtctga
    16381 caaccttctg aaaccgcccg tttttcagca gcttcaagca actgaccgcc taaccctttt
    16441 cctcggtatt cttttgctac gcaaaatcgt tccggcttga agcaagtagg tgttttcttt
    16501 tcataacgca aagtagctac tggtcttttt tgatcaagaa atagataata ggtttgttgc
    16561 gggatatctt ttccgtcgaa ttctagttct gcaggaatcc cttgttctaa tacaaaaact
    16621 tgatatctca aaaagcagct agctgcgcgt atccattcat ccgctccaac tatttttttc
    16681 atcatattcc tccttgtcat gaatagcaga gatgtattga ttcctttatt cttatcgtac
    16741 caaaaatcgt ggagtatgac caaaaggaaa tgaaaataaa aattgagata ttgacagtga
    16801 tcaatagaat gcgtatacta aataagtacg aaaaggatat gatttgtttt ttaagactca
    16861 cagaaaattc tttcttggct gaaaaaagaa tagtcactaa tcaaattgct ccctttttct
    16921 attttggtag aaataccgac gcagctcagc gttatcgagt ttaagaggta atgattacac
    16981 atcattgcaa tcaaggtggt accgcgagtt ttcgtccttg gctgtgatgg tgtttttttg
    17041 tatttaattt atagaaagca ggtaatgaca tgaaacaatt aagtagtgca caagttcgac
    17101 aaatgtttct tgatttcttc caatctaaag gacatacgat tgaacctagt gcgtctttga
    17161 tccccgtgaa cgatccaacc ttattgtgga tcaactctgg tgttgcaaca ttgaaaaaat
    17221 attttgatgg ttcagttgtg ccagaaaatc caagaatcac aaatgcccaa aaatctattc
    17281 gaacaaatga tatcgaaaat gtcggtaaaa cagcacggca ccatacgatg tttgagatgc
    17341 tgggtaattt ttcgattggt gactatttca aaaatgaagc catccactgg gcatgggaat
    17401 ttttgactga tccaaaatgg atggcattcg atccagaaaa actttatgta acagtttatc
    17461 caaaagatac agaagcaaaa cgcatctggc atgaagaagt tggcctgcca ctagatcaca
    17521 ttattgatgt agaagacaac ttctgggata tcggtgcagg cccatgtggt ccggatacgg
    17581 aaattttcta tgaccgtgga gaagcttata acgatttagc agaagacgat cctgaaaact
    17641 atccaggagg agaaaatgaa cgttatttag aaatctggaa cttagtattt tcagaattca
    17701 accataagcc tgataacaca tttgaaccac ttcctcataa aaacattgat acggggatgg
    17761 gattggaacg catggtctca atcgttcaag atgcgtcaac gaactttgag acagacttgt
    17821 ttatgcctat tatccacgcc gtagaaaagc taagcaaaca agtaaaatat ggtgaaaacc
    17881 cagtaacaga tatttcttac aaagtaatcg cagatcatat tcgcgccctt tcatttgcta
    17941 ttggcgacgg tgcattgcca tcgaatgaag ggagagggta tgtcttacgc cgtttgcttc
    18001 gtcgtgctgt catgcatggg aaaaaattag gtattgaaca agcattctta tatcgtttag
    18061 tccctgttgt tggggaaatc atggtaagtt attatcctga agtacttcaa caaaaagaat
    18121 ttattgaaaa agttatccgt acagaagaag aacgtttcaa tgaaacaatc aatgaaggtt
    18181 tggaaatctt aaatcaagtg atccaagaag taaaaaacga aaacgggacg actctagcag
    18241 gaaaagacat cttcaaacta tacgacacat atggcttccc ggtagaatta acggaagagg
    18301 tggcagaaga tgaaggactg aaagttgatc atgctggttt tgaaaaagaa atggatgcac
    18361 aacgcgaacg tgctcgctct gcccgcagta ctgaaaaatc aatgggggta caatctgctt
    18421 tattgacaga tatcaaagta gaaagtaaat tcattggata cacagaacaa gaatcagata
    18481 gtcaattatt ggtcatcgtt aaaggtgagt ctcttttgaa tgaagtcgca gaaggtgaag
    18541 ctcaaatgat ctttgatcaa acacctttct atgcagaaat gggtgggcaa gtagcggatc
    18601 aaggatggat catgaatgaa gtaggcgaaa tcgtagcagt tgttacagat gttcaaaaag
    18661 cgcctaatgg tcaatttatg cacaccgtag aagtaaaagc accaatgagt gaaggacaaa
    18721 cctatcatct attgatcgat caatcgatgc gcaatcggat catcaaaaac catacagcaa
    18781 cccatctact gcacaaggca ttgaaagaag tacttggcga gcatgcaaat caagctggtt
    18841 ctttagtcgc tcctggacat ttacgattcg actttaccca ctttggtcaa atcacttcag
    18901 atgaattagc tgaaatggaa agaatcgtca acgaaaaaat ctgggaagct atcccagttg
    18961 aaacaattga aacagatatc gatacagcca aaaacatggg tgcaatggct ttgtttggtg
    19021 aaaaatacgg acatgacgtt cgagtagtaa atattggcgg ctggtcaatc gaactgtgcg
    19081 gaggcacaca tgtagcaaat acagaaaaca tcggaatctt caaaatcgtt tctgaatcag
    19141 gaattggtgc aggcgtgcga cgaatcgagg ctgtaacaag taaagaagcg tacgaactac
    19201 ttgaagaaga agaaaaacaa ctaaaacaag tagcaacaat tgttaaatca cctcaattga
    19261 aagaagttgt ttcaaaagcg gaacaattgc aacagcaagt aagagagcta caaaaggaaa
    19321 atgaacaatt agctagcaag ttagcgaatc aacaagctgg tgatgtattc aaagatccag
    19381 aaacgatcaa tggaaaaaca atcattgcag cacaagtaaa agtgaaagat atgaatcagc
    19441 tgcgtcaatt agctgaccaa tggaaacaaa aaaatatttc agatattctt gttttagcaa
    19501 acgaacaaga aggaaaagtc agtctgctag cggcaatgac gaaagaatca aatgaagctg
    19561 gactaaaagc tggcgacttg attaaagcaa tcgcaccgaa agtaggcggt ggcggtggtg
    19621 gacgtccaga catggcacaa gctggcggga aaaatcctgc tggtattcca gatgcactta
    19681 aagctgcgaa agaatgggtt gcgcaacaat agggcgttta cgtttgacag aaaaacaaaa
    19741 gttcaaacat tgaacgaaac atgattatgg gggcgaagac aaacaagtct tgccctcata
    19801 cttttttcaa ttggatggcg catctaatca aatggtcgga ttcggatggt ctttagttga
    19861 cgaaagtgac agaaattttt acgattagta taaataaaaa ggggtgaaaa aatgaatagt
    19921 agaaataaga cctttcgact agtcttacgt gcgatcttgt tagcaatcat catcgtacaa
    19981 gcgatggttc cttggctggg gtttattcca ttaggattca ttagtttaac aattatccat
    20041 atcacagtaa ttattgctgc agtagtatta ggcccaaaag atggcatggt gattggcttg
    20101 ttttggggaa tcgctacaat tgttcgtgcc tatgccatgc caacaacgcc atttgataca
    20161 ttagtgttca cgaatccgat tatctcggta gttcccagag tacttgttgg cttagtagca
    20221 ggcttggttt tccactggat ttatcaaaaa aataaatcaa taacgatcag ctcagccttt
    20281 gcaggggtac tgggttcatt ggtcaataca gtcttagtgt taggattcat gggattattt
    20341 tatacaggtg caacggcaga tgcctatggt gtagactctg ctcttttatt taaaacctta
    20401 gcaggggttg cagcaatcaa tgggatacca gaagcaatag gtgcgggaat cataacacca
    20461 ttactagcaa aagcgttatt tgcagcaaca ccgttgaaac cagaataatg accatctttt
    20521 aatagtaata ggacgcacga tagatagaaa aaggaagaga ggagaggaaa acaatcgtcc
    20581 tctccatctt tttacctgag caacaagcaa actagaaggg gtaaaaaact tggctaagaa
    20641 acaagaagaa aaaaagaaac aaataccgca acagcggaca aaaaaaggag tactagcggg
    20701 tatcttagga ttggttacga atctgatctt gtttgtctct aagctaatga tcgggctttt
    20761 ttcaggaagt gtctccatta tggctgatgc agtgaacagt ctatcagata ctgcttcatc
    20821 catattgact ttgatcggat ttaaaattgc tgcaaagcca gcagatcagg aacatcctta
    20881 tggacacgaa cgcttcgaat atatcagtgg gttatttgtt tcgatcatca ttacttatgt
    20941 aggatttcaa tttttagagt cttctgctga aaaaatcatc catccagaaa atgtctcttt
    21001 aacacccatc gtcttttttg tcctaatcat ttccatcttt ttgaaatttg cacaaggcaa
    21061 aatgtatcag gttattgcgc aatccattga ttctgaaaca ttgagagcta cagcaacaga
    21121 tagttataat gatgtcttta ctacactggc tgtattgctt tcagcagcaa tcgaatgggt
    21181 aactggatgg cgtattgatg gatatatagg ttttgcttta gcattatata tcctttatag
    21241 tggattgacg atgcttcgaa attttgtata cgaactatta ggaagtcgcc caacgccagc
    21301 agagattgaa gaaatggaag agcatttaaa tgcttgcgat gatatacttg gctatcatga
    21361 tttattggtg cataactatg gacccaataa acgctttgca tcagtccaca tcgaagtaga
    21421 tgaatcaatg aatttgaatg tggctcataa gattattgat ctgatagaaa aagactttaa
    21481 aaagtctttg gatgtggaac ttgtctgtca cttggatcca atgcctgttg ataacgaaaa
    21541 atactataat atcttgcggc agttaaagca aattcttctt gaaacaggta cagggcttaa
    21601 tattcatgat tttagagtaa ttcatcaagg aaaaacgctg caatttgatg tagtggtccc
    21661 tgagaaattc cagtttgttg atacagaact agaaagaatc ttacgtagtc aaatagaaga
    21721 aaaaatcgga gattaccaat tggaaattac gtttgatcac aattatcttt tatattaaat
    21781 gagaaactat aaaaagactc cttaaagttt ttaatttaaa gagtcttttt ttcttttaat
    21841 tgtttatgga gagcctgacg tgcaagatta tctgctcctt tatttttgtt ttcgggcaac
    21901 cattgcagga tcaataaaga aaattcggat agaagttctt ggattctttg aaggtaggga
    21961 aggaagagct cgttcttcgt tgcgtttttg tcgaccgtag aaactagtgt tttgctgtct
    22021 gaaaaacaga aaatcgtttc attctgtaag tcgtttttct ttagaaattc taaagttttt
    22081 aaaaagactt cgaactctgc ttgatggttt gtccctatag aaagcgggat agatagctgt
    22141 tcttgtttac cttgatagac aatcaagatt ccacctccac taggaccagg gttgcctttt
    22201 gtagaggcat caatgtatac ttttaacata aaaagagatt ctcctttttg ttcaaactaa
    22261 tgttatggta agattatatc attactgaat cggtgagagg ttagagaatg aaaaaaaata
    22321 ttcgatggca gcctgaactg gcacatacga tcatctattg gtcattgacc ttgatcattc
    22381 tgttttttag tctgattctt tctttagaaa acacaggtcc ttattggaaa agcaattttg
    22441 ttttaggtat ttttcttgtt tttctatttc taggtttgcg gcgttctttt attttaaaat
    22501 caaactctgt gattatacgt tatgctgctt tttggaaaga ccgagaaata tctgtcggac
    22561 agattcgatc tttgacgcca actaatactg gggtgcaaat gatgttgcaa gatgagaaag
    22621 ccccatctgt tttcatgatg cgaaaaaaag atcaagtagt cctttttgat cattttgaaa
    22681 aaaacggacc caaatcagaa tcaattcctt atccttatac gaaaaaaaga aaaagctaat
    22741 tttaaaaatc agaacctaaa aaaacgaatt tttacaggaa aagaaaagag tgagacgtac
    22801 ggttcatccc gtttagcgtc tcactctatt tgtttatcta ttcatgcata ctagtatcgg
    22861 tagtctgaac gttgcctttc ttgacatggg cagcttgtgt tcctcgattc ccttcgatga
    22921 tctcaaattc gaccacttga ttttcttcca aagttttgaa tccctcttct tcaatcgcag
    22981 taaaatgtac aaaaatttct tctgtgtcat cataactgat aaagccgtat ccttttttgt
    23041 tgtcaaacca ttttacaatt cctgtggtca aagcactcac gacctttcta aagtctgatc
    23101 atagccttca ttataaagag tatttataag tacgtcaaat aaattaaaaa cctgtaattt
    23161 tatgtttttt ttctgaataa aaaaaggtat aattggcata tcttaacaaa gaagatattt
    23221 tgttgaataa agttcgtgaa aaggggtaat caaatgaaga atgatctgca aatttcacaa
    23281 gaaacagagc tagaaccgat tgttgaagtg gctagtaaat tagatcttaa ggaagacgat
    23341 ttggaactgt atgggaaata taaggcgaaa atcaattttt ctggaatcaa tcgatcaaag
    23401 gataacgctg aggggcattt gatcttagtt acttccatca atccaacacc agctggcgaa
    23461 ggaaagtcaa cgatcactgt agggttagga gacgcgctca ataaaatcaa taaaaaatct
    23521 gttattgcat tgcgagaacc ctcacttggt cctgtgatgg gaatcaaagg tggtgctgca
    23581 ggcggaggat atgcacaggt attgcctatg gaagatatca acttgcattt tactggtgac
    23641 atgcatgcta tcacaacagc taataatgca ttatctgctc ttttagataa tcatattcat
    23701 caaggaaatg aattgaacat cgatgcccgc cgagtgattt ggaaacgggt agtcgatctc
    23761 aatgatcgtg aactgcgtaa agtaattgtt ggcttgggtg ggccaatcca aggtgttcca
    23821 agagaagatg ggtttgatat cacagttgcc agcgaaatca tggctatctt gtgtcttgcg
    23881 gcagaccttg aagatttaaa ggctcgtctt gcaaggatcg ttgtcggcta tacatttgac
    23941 cgtcagcctg tgactgctgg agacttgaaa gcagaaggcg ctctggcatt actgctgaaa
    24001 gatgcaatca agccgaatct tgtacaaaca atttacggaa caccagcttt tgtccacggg
    24061 ggaccgtttg ccaatatcgc tcatggctgc aatagtattt tagctaccaa gacagcattg
    24121 agattagcag attacgttgt cacagaggca ggatttggtg cagatcttgg cggagagaaa
    24181 tttttagata tcaaagtacc taacttgaaa aaagcgccgg atgctgtggt gattgtcgcc
    24241 actgtccgtg cattgaaaat gcatggcgga atgaaaaaag atgaacttaa aaatgagaat
    24301 cttgatgctt tgaaagtagg ttttgcaaat ttaaagcgcc atatacgtaa tatggaacaa
    24361 tatcagctac ctgtgatcgt cgcaatcaat gaattcgtaa cagatacaga caatgaactc
    24421 acactgttgg agcacttgtg tgaaaatcag gggattcttg ccaagagagc gagtgtatgg
    24481 gcagatggag cagaaggcgg cgttgattta gcaaaagctg tagtacgatt gattgataga
    24541 aaagaagcta attacaagcc attataccgc ctagaagaaa cgatacaaga aaagacagaa
    24601 acaatcgtca agaaaattta cggaggaaat ggtgttgttt tcagcaaaaa agcgcaaaaa
    24661 caaatagaag agtttacgaa gaatggatgg gacaaactgc cgatttgtat ggcaaaaaca
    24721 cagtattcat tttctgatga tccttcactg ctaggtgcac cggaagattt caccatcacg
    24781 atccgcgaaa tcatcccaaa attaggggca ggatttttag tagccttgac tggagatgtt
    24841 atgacgatgc ctggtttgcc taaaaaacct gctgcattga atatggacgt gacaaatgac
    24901 ggagaagtgt tgggattgtt ttaagcttga ttataatcta ccatcgaagg agctgtattt
    24961 tgactacagc ttcttttttt agacgatagg gaattaattt tgtttacact aggatcattc
    25021 tttttcttta ttgtattttg agctatacta aagcataata ggagggtatg atgaaaacga
    25081 catttataag aaaaaccggt ttttacggta tgggtagctc gattcagcta agaatcaaca
    25141 atgaaaaacg cagtgttctc agtcatgaac aaaagataac gcttgatctg gaagtgccct
    25201 ttacgatgca ggtcaccttt tattggctaa aaagccccgt ttatacagtg aaagaagcaa
    25261 agcctgctta tgtgatcaca atgaattact tgattcttca aatctatccg tttttatttc
    25321 tagcaactgg gttgggggcc gttttcctac aaaatttatt tttcagtttt tttgcagtta
    25381 ttgtgttgat tgcgttcttt ttctatataa aaaaccgggc atatttgatc aaggaggaaa
    25441 attatggcaa actctgatct ttttttaatc agtttcaatc gaatcgaaaa atggatgaga
    25501 gacgagatgg gaaatgcacg aaatatggga tttaccgaac ttgtcagacg cctagctcaa
    25561 agaaaacaat taatgatcag aaaatacgaa gatgatcttt tacagcttgc tcagttaagg
    25621 aatgcgatcg tccatgatcg gatcgcagtc gattttgtca ttgcagagcc aaatgattgg
    25681 acagtcaagc gaatccagct gatcgaacaa gaactgattc agcctgaaac cgtgctgcct
    25741 cgatttgcaa aacgtgtaac aggttttgaa caagatcttc cgttacttga actgttaaag
    25801 atagtagcag aaaaaaggta ttcacaattt ccgttataca ataaaggaaa atttgtagca
    25861 ttgattactt tacgtaatat cgggttttgg ctagcaaaag aaagccaaaa aggtccggtt
    25921 gatttaacaa ataaacgagc gttggatttg atcatccaaa acggaaagta cacaaattat
    25981 cattttgttc cagctaaaac acatatttac gaggttgaag cgatgtttcg tgagcaaggg
    26041 actttggaag ccgtacttat cactaaagat gggaatcccg atggtaatct gctcgggatc
    26101 gtacgacctc gtgatattta taaacaaata gaaaaggatt gattttttgt taattattta
    26161 ttggatattc agcgctttgc tagtaggagt ggatcagttg gtcaaatggt ggatcactga
    26221 taatctagcg cttggagaga cgaaaaatct tataccaggt attttgtcac tgaatcatat
    26281 tcgaaacact ggagccgcat ggagtatgct agaagggaag atgtggttct ttactatagt
    26341 gacactcatt gcagtcgttg tgattctcac attgatgatt aaaaatcgtg aaaaaggtaa
    26401 tcgctggttc atgattggat tgagtttgat tctagctggt gcgattggca attttatcga
    26461 ccgtattcgt cttggctatg tagtagatat gttccagaca gattttatga gctttccgat
    26521 ttttaacgta gcagatgtga cacttgtatg tggagtgatt tgtatgttaa tttatattat
    26581 tttagatgaa aaggatcaaa gaaaaaaatg acagaactta aaacgacgat tcaccatgaa
    26641 tctggtagga tcgataaagt attgactaat ttattcaccg attacagtcg ttcgcaaatt
    26701 cagctgtggc tgaaaaatgg agcggtttct gtcaatggac agccagtcaa agcaaattac
    26761 aaagtaaaac aaggagacga gattactgtc ttgatccccg aaccagaaac attagatgtg
    26821 gtcccagaaa atatcccgtt gacgatcgtt tacgaagatg aagatgtagc ggtgataaac
    26881 aaacctcaag gaatggtcgt tcatccttca gcaggtcata cgcatggcac aatggtcaat
    26941 gcgctgatgt accatatcaa ggatctttct acgatcaacg gcgttattcg tccaggtatt
    27001 gttcaccgaa tcgacaaaga tacttccgga ctgctaatgg ttgccaaaaa tgaccaggca
    27061 cacgaatcat tagctcagca attaaaagat aagacctctt tgcgcaaata tgttgcttta
    27121 gtccatggag agattcctca cgaaaaagga accatcaatg cgccaatcgg ccgttcgaag
    27181 gaaaaccgga aaatgcaggc gataagagaa gatgggaagt cggctgtcac acattttact
    27241 gtattggaac gtttcgaagg atttactctg gttgaattaa aactagaaac cggaagaaca
    27301 caccagatcc gtgtgcatat gaagtatatc ggttatccgt tagctggaga tccagtatac
    27361 ggaccgaaga aaactttgaa aggcaatggt cagtttctcc atgcgcaaat gttaggtttt
    27421 acgcacccga ctacaaacga aaagatgatc tttgaagcac ctcttccaga agtttttgaa
    27481 aaaactttag aaaaattaag aaaaaacatt gcattttaat ttagataacg gtatagtaat
    27541 taacaacgaa agcataaaac ttaatagaat tcctttaagt ttagtcccgt gaggctaaga
    27601 agggtgggag caaggatacg tatggtgcgc cataagtaga ttgctcattg acatagtata
    27661 gctatcagtg tatcagtata cgctcagtct aaacattcct cctgtcctct tagggcagga
    27721 gtttttttat gcaaaaaagt agggggaatg aaaatgcaag caaaagaagt agtagatcag
    27781 gtaaccatga agcgagcatt gactcgaatt acttatgaga ttatcgagag aaaccatagt
    27841 atccaagata tcgtattggt aggtatcaaa acacgaggaa tctacattgc atcaagaatt
    27901 gccgaacgcc tgaagcagct agaagatatc gatattcctg taggagaact ggatatcact
    27961 ttataccgag atgacaaaaa agaaaatcca gaagaaccgg aacttcattc ttctgacatc
    28021 ccagtttcct tagaaggaaa agaagttatt ttgattgatg atgttttgta tacaggacga
    28081 acgattcgag cagcaatgga cgcagtaatg gattttggca gaccaagaaa aatttcatta
    28141 gcagtcctgg tcgatcgtgg gcatcgagaa ttgccaatcc gtgcagatta tgttgggaag
    28201 aacattccaa catctcgggc ggaagagatt ttagtagaga tgcaagagct tgatggacaa
    28261 gatcgcatca tgattttgaa agaggaagat tagaggagag agaaacatgt cggagaaaaa
    28321 aggatttcat aatgatgacg tggtgttaga tatccacgat cgcccaaccc caggacattg
    28381 ggtaggatta agtttacaac acttgttcac gatgttcgga gcaaccgtgt tagtccccat
    28441 cttagtaggt atcgatccag gtattgcgct agtcagttca ggactaggta cattggtgta
    28501 cttatttact actaaaggaa agatacctgc gtacttagga agcagcttcg ctttcattgc
    28561 agcgatgcag cttttgatga aaacagatgg ttatccagcg attgcacaag gagcagtcac
    28621 aactggatta gtctatttga ttgttgcgct gatcatcaaa aagatcggtt cagcatggct
    28681 ggataaaatc ttgcctccaa tcgtcgtcgg tccagtcatc atggttatcg ggttgggact
    28741 tgcggcaaat gcagcaacaa atgctatgta taatcagggc acgtatgatt tcaagtatat
    28801 tgctgtagcg ctgatcacat taggattaac aattttcttc aacatgtggc taagaggttt
    28861 tctcggattg attccgattt tactaggtat tattagcgga tacatcgtag ccttgctgtt
    28921 tggaatcgtg gatactaagc caatcgtaga cgctgcctgg tttgctgttc ctaactttga
    28981 gattcccttt gtacaatatg aaccaaagct atatcttgga gcaatcacaa caatggcacc
    29041 catcgctttt gtgacgatga cagagcatat cggccatctt atggtattga ataaactaac
    29101 taaacgtaat ttctttgaag aaccgggtct tcataagacg ttgacaggtg atggattagc
    29161 acaaatcgtg gcagcctttg tcggaggtcc tcctgtaaca agttacggtg aaaacattgg
    29221 tgttctggct atcacacgtg tccatagcgt gtttgtgatc ggtggagctg cagtatttgc
    29281 agtcatttta ggattcgtag gaaaattaag cgctttgatt ttaagtatcc ctggaccagt
    29341 catctcagga atcagtttta tcttatttgg agtaattgcc gcaagtggaa tgaagatttt
    29401 aatcgagaac aaaatcgatt ttgataaaaa gaaaaatctg ttgattgctt cagttatctt
    29461 agtagtagga attggcggac tcgtctttga agtaggcaca tttacattgt cggcaatggc
    29521 attagcaact gtacttggca tcattttaaa tcttatctta ccagaaactt cacgaagcga
    29581 agaaaactaa ggaggaaaca ttatgatcat caaatcagaa cgtatcagct taaagcattt
    29641 attgaccgtt gaggcactta ctgatcaaga agttatgggc ttgatccgca gagggcagga
    29701 attcaagcaa ggtgctaatt ggactccgca aaagcgccag tactttgcta caaacctctt
    29761 tttcgaaaat agtactcgca cacataaaag cttcgatgta gcggagaaaa aactagggct
    29821 agaagtgatt gaatttgaag caagcacaag ctctgtccaa aaaggggaaa cactatacga
    29881 tacagtactg acgatgtcag cattaggcgt agacgtagcg gttatccgtc atggtgatga
    29941 gaactattat gacgaactga tacaaagtaa aacaatccag tgttcgatta tcaatggagg
    30001 agacggaagc ggacagcatc caacccaatg cttgcttgat ttgatgacca tttatgaaga
    30061 atttggtcat tttgaaggtc tgaaagttgc catcgtaggt gatatcactc actctcgcgt
    30121 agctaaatcc aatatgcaga tgttaaaacg tctaggcgca caagtattct tctcaggtcc
    30181 tagagaatgg tacgacgatg aattcgaagt gtacggacat tatatgccat tggatgaact
    30241 actagatcaa gttgatgtca tgatgatgct tcgggtgcaa cacgaacgcc atgatggaaa
    30301 agaaagtttc tcaaaagaag gctaccatca ggaacatggg ttaacagttg agcgtgcgaa
    30361 gaagttgcaa aaacatgcaa tcattatgca cccagcacca gttaaccgtg atgtagaatt
    30421 agctgattca ttagtggaag gattacagtc gcggatcgtt cagcaaatga gcaatggagt
    30481 attcgtcaga atggcaatct tagaagcagt gctaaatgga aaagcgtaat tataggagga
    30541 tgaaaaaatg aaaacactca ttaaaaacgg tcagatcaat acacgaaaaa atatgacgac
    30601 accagcagaa atttggatag aagatgggcg aatcaaagca atcggtacag gattctcaga
    30661 agccgaattt gatgaagtgt ttgatgcaaa gggacaattg atcacacctg gtttagtgga
    30721 cgtccatgtt catttgagag agccaggatt tacatacaag gagacgatcg aagctgggac
    30781 tcgctcagct gctcgtggcg gtttcactac tgtatgcgcg atgccgaatt taaatcctgt
    30841 acctgatacg gcagaaaagc tgagacaagt atatgacatc attagaaaag atgcagttgt
    30901 gaaggtattg cagtatgcgc caatcacaga aaatctgcga agtgagaaat tagttgatca
    30961 agaagcactg attgaagaag gtgcttttgc tttcacaaat gacggtgtag gtgtccaaac
    31021 agctgggacg atgtatctgg ctatgaaaga agctgcaaaa aacaataaag ccctagtagc
    31081 ccatacagaa gatgaatctt tattatttgg cggcgttatg cacgctggca agaaggcaga
    31141 agaacttggt cttcccggga tcttaagcgt aaccgagtct tctcaaattg ctagagatct
    31201 gttgctagct gaagcaactg gtgttcatta ccatgtttgc catgtttcta ctaaagaaag
    31261 tgtccgtgta atcagagacg caaaaaaagc aggaatccac gtaacagcag aagtttcacc
    31321 ccatcatctt attttgatcg acgaagatat tcccgaagat ttcggctttt ggaaaatgaa
    31381 cccgccgcta agaggacggg aggacagaga agctttgatc gaaggcttgc ttgacggtac
    31441 gatcgattgc atcgctacag accatgcccc ccatggatta gaagaaaaaa gccaaagttt
    31501 catgaaatca ccatttggta tcgtaggaag cgaaacagcg tttcaactga tctatactca
    31561 ttttgtagaa actggtcgat tcacgttgga acaagtgatc aattggttgg cagttaaacc
    31621 ggctgagatt tttggtttga atgcaggaac gctaacggtt ggtgcccccg ctgatgtagc
    31681 agtttttgat attacacaaa cttgcacgat cgataaagaa gactttttat caaaagggga
    31741 gaatacacct tttatcggct ggaaagtaaa aggcgaaaca caaatgactt ttgtcaacgg
    31801 aaaacttgtg tggcagaagg gagaataaaa gtatggaacg tttgcttata ttagaagatg
    31861 gaacagtttt cgaaggaaag gcttttggtg cagatgctag tgtcttcgga gaaatcgttt
    31921 ttacaacaag tatgactggt tatcaagaaa ctatcactga tcaaagtttt aacggtcaaa
    31981 tcattacatt tacctatcct atggtgggta attatggggt aaatagagat gattatgaat
    32041 cgatttctcc aacatgcaaa ggagtagtag taaaagaaca tgcacgtgta gcttcaaact
    32101 ggcgaaacca aatgacgcta gattcatttt tgaaacaaaa aggaattcct ggaatatcag
    32161 gtatcgatac gcgcgccctt actcgcaaaa tccgtgaagt cggaacgatg aaagctagta
    32221 tagttgatgc tggagattcc ttcgaacatg catttgatca gctgaaagca accgttctag
    32281 caaccaatca agtgcagcaa gtctcaacta acaaacctta tccaagtcca ggaacaggtc
    32341 ggaaagtcat tgtcattgac ttcggcttga aacatagtat tttacgagaa ttgagtaaaa
    32401 gaaactgtaa cctcacagta cttccctata acacagatgc cgagacgatt ttagcgctat
    32461 caccagacgg agtcatgctg acgaatggac ctggtgatcc aaaagatgtg ccagaagcaa
    32521 tcgaaatgat caaaaaaatc caaggaaaag taccgatttt tggtatttgt ctcggtcacc
    32581 agttatttgc tttagccaac ggtgcagata cctataaaat gaaattcgga catcgaggat
    32641 taaatcaccc ggtacgagaa gtagcgaccg gaagaattga ttttacttct caaaatcatg
    32701 gctatgcagt cgatgaagcc acgattgatc cagaaaaact catcgtaact catgtagaag
    32761 taaatgacgg aacagtagaa ggagtcaaac acagagatta tcccgctttc agtgtgcagt
    32821 ttcatccaga tgctgcccct ggtccgcatg atgcactaca tttatttgat gaatttatgg
    32881 aaatgatgga cgcaggaaag gagcagtaaa cagatgccga aacgtacaga tatcaagaaa
    32941 attatggtga ttggatctgg tccgatcatc attggtcaag ccgcagaatt tgactacgca
    33001 ggaacacaag cctgccttgc cttaaaagaa gaaggatatg aagtggtttt agtaaattct
    33061 aatcctgcga cgatcatgac ggataaagaa gtggcagatc aagtatatat cgaaccgatc
    33121 acacttgaat ttgtatcaag gattcttcga aaagagcgtc cagatgctct ccttccaact
    33181 ttaggcggac agacaggcct caatatggca atggagctgg cagcatcagg tattttagaa
    33241 gaattgaaca tagaacttct aggaacaaag ctttctgcga tcgatcaagc agaagatcgt
    33301 gatctattca aacagttgat gaaagaattg aatcagccaa tccccgagtc tgaaatcgtc
    33361 aatacagtag aagaagctgt agattttgca aataaaatcg gctatcctgt catcgttcgt
    33421 ccagccttta ctttaggcgg aactggtgga ggtatgtgtg aaaatgaaga agaattacgt
    33481 atcgtatcag aaaatggatt aagtctttcc cccgctaccc aatgtttgat tgaacgaagt
    33541 attgcaggtt ttaaagaaat tgaatatgaa gtgatgagag attcggctga taatgcaatc
    33601 gttgtctgca atatggaaaa ttttgacccg gtgggtatcc atacaggtga ttctatcgta
    33661 tttgccccta gtcaaacatt gtctgaccat gaatatcaaa tgcttcgtga tgcttctctt
    33721 tcgattattc gagcacttaa gatcgaggga ggctgtaatg tccaactcgc tcttgatcca
    33781 catagtttta attattatgt gattgaagtc aatccgcgtg tttctcgttc atctgcgtta
    33841 gcaagtaagg cgactggcta tccgatcgct aagctagctg ctaaaatagc gcttggatta
    33901 acattagacg aaatgaaaaa tcctgttaca ggaacgactt ttgcagaatt tgaacctgca
    33961 ttagattatg ttgtagctaa aattcctcgc tggccgtttg ataagtttga aaacggagaa
    34021 cggcttttag gcacgcagat gaaagcaact ggtgaagtga tggctatcgg tcggaatatc
    34081 gaagagtctt tattgaaagc tgtgcgttct ttagaaatcg gtacacacca tatcgaactt
    34141 cctgagttgg cagacatcac ggacgatgac ctaatccaaa aagtggttcg agcacaagat
    34201 gatcgcctgt tttatgtagc tgaagctatt agaagagggt acaccataga agaattagcg
    34261 gatttgacaa aaatcgattt attcttttta gataaactgt tgcatatcgt agaaatcgaa
    34321 cagcagttga aacagcagcc gttggatgac aagatattga aaacagcgaa acaaaacggt
    34381 tttacagaca aaaaagtggc ggagctgtgg gaaataaacg aagaagccgt tcgccagatg
    34441 agaaaagaaa atgatttgat tcctgtatat aagatggttg acacttgtgc agcggaattc
    34501 gaatcaaaaa caccttattt ttatagttcc tatgaatatg aaaatgaaag ttcacgatca
    34561 gaaaagccct caattctagt actgggatct ggtcctatcc gtatcggaca aggggttgaa
    34621 tttgactatg cgactgtgca ttctgtcaaa gcaatccagc aggcaggata cgaagcaatc
    34681 atcatgaaca gcaatcctga aacagtttcg actgactttt ctatttcgga taaattatat
    34741 tttgagccat tgaccttcga agatgtgatg aatgtgatcg agctagaaca gccgatagga
    34801 gtaatcgttc aatttggtgg tcagacagca atcaacttag ccgaaccttt gactgctgct
    34861 ggtgtaaaaa tcttagggac aacgatcgaa gacttggatc gtgcggaaaa tcgtgattta
    34921 ttcgagcaag cgctgaaaga gttggatatt cctcaacctt taggggatac tgctacaagt
    34981 aaagaagaag ccgtggttat cgcttcgaga attggttatc cagttcttgt tcgtccaagc
    35041 tatgtattag gcggacgtgc gatggaaatc gttgaaaacc agcgagattt agaagattac
    35101 atggaacatg ctgtgaaagc atctccagaa catccagtac tagtggaccg ttacttgctt
    35161 ggtggtgaat gcgaagtgga tgcgatttgt gacggtgaaa cagtactcat cccagggatc
    35221 atggagcata ttgagcgtgc tggtgtccat tcaggggatt caatggctgt gtatccacct
    35281 caaacattat ctgaatcgat caagaaaacg atcgaagatt acacgatccg cttagcatta
    35341 ggtttgaatt gtgtcgggat gatgaatatc cagtttgtca tccatgaaaa tgaggtttat
    35401 gtgatcgaag tcaatcctcg agcaagtcgg actgttccat tcttaagtaa ggtgacagga
    35461 attccaatgg cacaaattgc gacgaaagct attttgggag agaaactaaa gacacttggt
    35521 tatgaaaacg gactttatcc agaaacaaaa gaagttcatg ttaaagcacc tgtcttttct
    35581 tttacgaaat tgcagaaagt tgatacttac ttagggcctg aaatgaaatc gactggagaa
    35641 gtaatgggat ctgacagaca attagacaaa gctttgtaca aggcattcga agcttctgga
    35701 ttacacttgc ctgaatatgg agccgtatta tttacgattg cggacgaaac aaaagaagaa
    35761 gcactagaat tagctaaacg ttttacagaa attggctata gtctgatagg aacgaaacat
    35821 acagctgctt atttcgaaaa aaatggtctg gtcgttgaaa cagtagcgaa aatttctgag
    35881 gaagcagcag aaaaaaatgt agtcgactta ataagagatg gtaaaacaca agtggttgtc
    35941 aatacgattg ataaagatcg gggaaatgct tctaaagacg gtttcatcat ccgaagagaa
    36001 gcagttgaac atggtattcc attgtttact tctttagaca cagcagatgc gatcgttcga
    36061 gtgatggaat cccgtgcttt ttcaactaac cctatttaac ggcgagcaca tgttaagaca
    36121 aaaagacgaa aaaccgttgc aggaggggac agatgaaaca agaaatgatg gaaatcgttc
    36181 agcaaaaaca gctggcacca aagatttttg aaatgacatt gaaaggtgat ctagtaaaag
    36241 aaatgcagca accaggacag ttcttgcata ttctcgtgcc gcgtgcagac ttgctattac
    36301 gccgaccgat tagcttgaat catatcgacc acgaaacaaa tacttgccgt atcatttatc
    36361 gggtggaagg tgaggggact agagtcttct ctactctttc tgccggagac catttagatg
    36421 tcatgggtcc tttaggcaat ggttttgatt tgtccaatct tcatacagga gacgaagtat
    36481 ttataatcgg tggaggaatc ggtgtccctc ctctatatga gttatcaaaa cagttagtcg
    36541 aaaaaggaat taagccaacg cattttttag gttttgccac acatgaagtc gtttattatg
    36601 aaaaagaatt taatcgtcta ggagaaacaa gaatcgctac tgacgatgga acatacggta
    36661 tgcacggaaa tgtcggtaat ttattgttag gaacgaagac ggaacctaca gctgtttttg
    36721 catgtggttc aaacgggtta ttgaagacag tggaacagct gttttcttcg catcaaaatg
    36781 tccagttatc attagaatcc cgcatggcat gtgggatagg ggcttgctat gcttgcgttt
    36841 gtcatacgcc ggaagatgaa acaggaacaa aaagtgtgaa agtttgtgac gaaggaccga
    36901 tttttaaaat aggagaggta attatatgaa tgagaatcct ttagcggtca aacttcctgg
    36961 cctagaatta aaaaatccta tcatgccggc aagcggctgt ttcggttttg gcaaagaata
    37021 cggaaaatac tatgatttga atcagcttgg atcgatcatg gtcaaggcaa cgactccaaa
    37081 tgcccgcttt ggcaatccca cgccaagagt ggcggaaaca ccatctggca tgttgaatgc
    37141 aattggtttg caaaatcctg gtctagacgt tgttatgcat aaacttcttc cagaattaga
    37201 ggagtacaat gagttaccca tcatcgcaaa tgttgctggt gcttgtgaag aagactatgt
    37261 agaagtttgt agcaaaattg gcgaagcacc aaatgtgaaa gcaatcgagc tgaacatttc
    37321 ttgcccaaat gtcaaacatg gcggaattgc ttttggaaca gattctgatg ttgcttttca
    37381 attaacacaa gcagtaaaga aagtttcttc tgttcctgtc tatgtcaaat tgtccccaaa
    37441 tgtaacagac atcgttccaa tcgctcaggc aatcgaagct ggaggggcag atgggtttac
    37501 gatgatcaat actcttcttg gtatgcggat tgaccttaag acaagaaaac cgattttagc
    37561 caatcaaaca ggtggactct ccggaccagc aatcaagcct gtcgctattc gtttgattca
    37621 ccaagtagca agtatatcca gccttccaat cattgggatg ggcggcgtgc agacagtgga
    37681 tgatgttctt gagatgttta tggctggagc aagtgctgta gctataggga ccgcgaattt
    37741 caccgatccg tatatctgtc ctaaactaat taaagagctc cctgtgcgaa tgagcgaact
    37801 aggcatcgaa tcgctggaaa gattaatcaa agaagtgaga gaggaacgtg aacgatgaat
    37861 caactagacc aacgaccaat tattgcatta gatttttcaa ctaggcaaga ggtagaagac
    37921 tttcttcgat ttttcccgaa agaagaaaaa ttgtttgtga aaatcggaat ggaattgttc
    37981 taccaagaag gacctgaaat cgtacgttat ctaaaagagg ctggtcataa tgtattttta
    38041 gatctgaaac ttcacgatat tccgaatacg gtcgaaaaag caatgcgggg attggcaaag
    38101 cttggtgttg atctcacctg tgttcatgcc gctggcggta ttcgtatgat ggaggcggca
    38161 atgcgaggat tagaagaggg gactccagaa ggtggaaaac gtccgctact tctagctatc
    38221 actcagctta cttctacaag tgaagaagaa atgcatgccg atcaactaat cgaagtttct
    38281 ttagaaaaaa gtgtgatcca ttatgctagc tgtgccaaaa aagcaggatt agatggtgtg
    38341 gtttcttctg cttgggaagt agaagcgatc aaagaaatgg caggagacga atttgtttgc
    38401 ttaactccgg gtataagacc agaaggaaca gtcgcaggag atcagacaag agtagtcaca
    38461 ccttctcagg caaaaaagat cggctcaact tttatcgtta taggtcgacc aatcactcag
    38521 tcaactaatc cttataaagc atatcaaacg attcaaacag aatggagcta acctaaaatg
    38581 aatgttgaac aaagtatcgc aaaagactta ttagaaattg aagcagtatt tttaaatcca
    38641 agtaatcctt ttacatgggc aagtggaatc aaaagcccaa tttactgtga taaccgaatc
    38701 acgatgagct atccaaaagt ccgaaaagag atcgcaaaag gattagcaag caaaatcaaa
    38761 gaagcttacc ctgaagtaca agtgattgct ggaactgcaa cagcaggaat ccctcatgcc
    38821 gcatgggtag cggaaatact ggatttgcca atggtctata ttcgaagtaa ggcgaaagat
    38881 catgggaaag gtaaccaaat cgaaggacgt atatttgaag gtcagaagat ggttgtgatc
    38941 gaggatttga tttctacagg cggaagtgtc ttggaagcag ctgaagcagc aaaaagagaa
    39001 ggtgcggata tccttggtgt tgcggctatc tttacgtatg agttgccaaa aggaaaagca
    39061 aatttcgaaa aagccgaaat ccctcttatg acgttgacca attattctgt attgattgaa
    39121 gcagctttag aagatcgtta tatcgatgag caagaattaa ctctgctaaa agaatggaaa
    39181 aaagatcctg aaaattggca gatttaagcg cgcttgatga agatagagaa atccgatttg
    39241 tgtctccaga atcatcaatt ggaatagcaa aaatccaaaa actgattgcg agaagcatct
    39301 tttcatcagg tatgaaaaga aacttctatc gtgatcagtt tttgattttt ttaaaattca
    39361 tttttctaag actggttagc agaaagaaga gaataatctc ttctcttttt tctattatat
    39421 atagattttc tctatttttt ggtaagggag ctgtttcaaa aatggtaaag agcggaaatg
    39481 ttgcgaatat cagttattta atgatatgat attcggaaaa agaaatctca aagggaggta
    39541 acttttatgg ggttgaatga atttatttta ggattgaaca atttagagac gatcacaagg
    39601 gcacctggat tttttaaata tacagagcat acagttgcag cacattcgta tcgtgtagca
    39661 tctattgcgc aagttctagg agacattgaa gaagcgaatg gacaaccgat cgattggaaa
    39721 ctgctctatg aaaaatcatt gaatcatgac tacacagagc gttttattgg tgatatcaaa
    39781 acgccggtga aatacgcaag caaagagtta agagggatgc tgcaaacagt tgaagaaaag
    39841 atgacggacg aattcatcaa ccaagagatt ccagctgagt tccaagaagt ttatcgccgt
    39901 cggttgttcg aaggaaaaga tgaaaccgta gaaggagaac tattagccat ttcagataag
    39961 gttgaccttc tttatgaatc ttttgatgaa atcatcaaaa ataacccgga aaaagtttat
    40021 aaggagatgt tcattgaggc agtgactacg attaaaagct ttgaacatcg gccttctgtt
    40081 cagtatttct ttaaagagat atttccggaa ctattgaacc aggaatttta tggaaaagag
    40141 gcgtttttag aaaccgtctc aacgattgta aagaaagaag aaaaataaaa atgatatgag
    40201 atgcaggggg aagtatagtt gaaaaagaat atggatgtcg aatggggcta ttctggagac
    40261 aaaggtccgg aaaattgggc gagtttatgt gattggtttg cgagaggtgc ggaatttcca
    40321 ttgcagtcgc ctattcattt gaccaagggg gaagaaacca aaatagaagg agacacgctt
    40381 tcctttcatt atcaaaagca acggtttact gacaaggaat tcaaaaacac gattcattta
    40441 gtaccttttg accaactcag ttatgtagag ttcaagaaag aacgctatta tttgacagat
    40501 attcatttcc acttgccttc tgaacatatc atcaacggaa aacaggaaga tattgaattt
    40561 cattttgttc atatgaacag caaaaaagag aatctagttg tgggtgtcat gtaccagcta
    40621 atggtacagg aaggttggct gtataatcaa gaaaatgggg aaagttggaa cttagaaaaa
    40681 catgagcatt gggtaaatcc ggatgtgttc tttccggagc aaaaaagcca tttccattat
    40741 attggctcat tgacaactcc gcctaccagc gggccaatcc aatggtttgt aatggatcat
    40801 gtaggaaaac tgaaccaaga gtttcttctc cccttcgatg gtcagtatgc tcgaccgaac
    40861 aaccgaccaa tccagccctt gaatggaaga gaaatttatt atttcgagga gtttgagcaa
    40921 tgaatattca gcagatgaaa tacgtggtcg ctgtagcaaa caatggtagt ttcagagaag
    40981 cggcaaagaa aatgtttgtc tcccagccaa gtctttcagc tggtatcaaa gaattagaaa
    41041 ccgagttagg gatcacgctg tttactcgaa cgaatcgagg ggcatattta acagaagaag
    41101 ggcaagactt cttaaatcgt gccgagaaaa tattggtcca gcttgataca ttagaaactc
    41161 actatctaac gagtgaaaag agagacaatt tttcgatcgc tgcccagcat tatgatttct
    41221 taggtccttt gaccgcacag ctactcgagg aattcaaaga cgagatcaaa caatttcgga
    41281 tcatggagac gacaacagcg agagtgatcg aagaagtcaa agagcagcac agtgagatcg
    41341 gcattattta tatgaatgag cataatcaag ctggtatcag aagatattta caacaaggag
    41401 agctaagtgg ccgcagcatt ggaaaattca aaacgcatat ttttttaagg caaggacatc
    41461 cattagctga acacgcagag atcaccaaag aagaattgtt atcttatcct caagttcgtt
    41521 ttacacagga tggaaataat ttcccgtatt tttatgaaga ccttatcgag atccctgatc
    41581 aagaaacagt gatttatacg agtgatcgag gtactttgat gaatatcgtt cttgaaactg
    41641 atgcttacgc ttcaggatct ggtattgtga ttggagggat tcgcgagcac ttgcggttaa
    41701 tccctttggc agaaggcgaa ctaaatgaat tttatatcat ttattcggcg aaacgtccgc
    41761 tcagtgaaat cgctcaaaga tttatcaaag agcttactcg gttactggac cagcagagct
    41821 aagaaaagca gatgagaacg ctgtttattg aacaccgttg atcaaaaaag aggggttgtg
    41881 acagaatttt gtcacaactc ctctttttgt agcaaacaat ttataactaa ttgctgttgt
    41941 gggaaagtac gcaaatatga ttctagaaac actcattgat tacctgtttt gtttcatagc
    42001 tgtaattttt tcttcttctg gtgtcacgat aatcgtcttt tggttttcat aaataacata
    42061 accaggcttg gcgccatttg gttttcggat atgctttacc tgtacaagat caaccggcac
    42121 ttgggcagag tatcggtatt ttgaaaaata ggcagctaac tcggcagctt ctgtaattgt
    42181 ttcatctgag ggcttgtcac ttttaatgat gacgtgcgaa ccgggtatat tttttgcatg
    42241 aagccaatag tcggtttttt tagctgtctt cattgtcagc tggtcatttt gaagattgtt
    42301 tttaccaaca agaatgagcg ttccgtcaga agaaaagtat tgatcaggct gagatggttt
    42361 cttacgtttt tgttttttta agttcttctt tttcaggtat ccttctgaag tcaattcttc
    42421 tttaattgct tcgatatcca ttgggccggc aatttccagt tgagataaga cagattcaag
    42481 atattggatt tcatctttgg cttcttggat ttgttcgcca atcagcttaa cagcgttctt
    42541 cagcttttgg tatcgatgga aatatttctg tgcattttga tttggtgtca atgccggatc
    42601 tagtgctatt ttgattggcc gatcttcttc ataataatta ggtaaaacaa cttcttttgc
    42661 gccgcgtggt acttgtgtca gaaaagtagt aagtagttcg ccatttctac ggtaattctc
    42721 agcgttttcg gattctttga gtgtttgttc acgttttttc aatttatttt tatttcgttt
    42781 tagttcattt tcgatttttc gaatcagttc tccgccttgt tgttttgccc gatctttctc
    42841 tgctttttcg tgataatagc tatctaataa ttgactgagt gttggataag ctgttacagc
    42901 agatgcctgt tcggacaata cctgataagc aatcggtgtg aaaaaatctt tattttccgt
    42961 ttcgtaaaaa gtaggaatag gctgatggct gatggctgag aaaaattcgt cccatacaac
    43021 catcttttca ttaggacgtt ccagtaatct tttggtcagc tcttgagcga tatcgaaacc
    43081 gattccctga aactgacgtt ggattgcttt tggatctagt tcagtttggg aaagccgctg
    43141 aaagattttt tctttctcgg agctaaatgg gttcaagacc tcttgttttg gcggagcaac
    43201 atattctaca ccaggaagca aagaacgata agtgttttga gagcttccaa tgtgtttgat
    43261 cgcatccagt atttttccag tttctcgatt aactaagacg atcgtactat gacgtcccat
    43321 taattcaacg atcaatatga tattttgtag atcacccaac tcgtttcgtt tagcaaagtg
    43381 gaaatggatc actcgatcat tttcgatttg ttcgatgtct tctaaaatag cgccatccaa
    43441 atattttcga agcatcatca cgaagttagg cggattatca ggattctgat aatcgatttg
    43501 ggtaatctgt acacgagcat aacttgggtg cgcagataat aataatcgat ggtttttgcc
    43561 gcgtgaacga atcaccagca cgatttcatt ttcataaggc tgatggattt tcgagatccg
    43621 gccagatagt aacgtttcac gcaattcgtt gaccatcgcg tgggtaaata caccatcaaa
    43681 ggacatggtt cattcctcat ttcttttcat aagtatattc attataacga aaaaaagaag
    43741 aaaaaccaat ttggccgaat agtaaagcaa aagtctagaa aaatcttgga ctaattacat
    43801 aaaaaacagt gtttctatcg ttgaaatcca aatttttagg tataataaaa aataggagaa
    43861 aaagtagaaa agaaggtgtt tgttatttgg gaagtagtta aatcgagagt acaagaaatg
    43921 aatgaacatt tctcagtgtc attcgcagaa aatccaagta agtattttcg acgtcttgtt
    43981 tttctgctca tcttatcatt gctgttaaca gttgcaatgt ttttattgcc ggaaaaaatc
    44041 cgagaagagc aagtaaatag tctcgtttat tcgtccatac caaccagcaa attgaaacct
    44101 tttgattaca gcaaagctga tcagaaaatc aaacaaagcc gggcgatatc ggtgatgttc
    44161 agcaaaccga acgggaaaac ctatcaggac atgcttgatg tttttaatga tcctgagctt
    44221 atggaagaat tgaatcgacc gatcttttat tatccgatcg tttatgatgt acatgaactg
    44281 gaacaaaaat ataacatacg aacggatgaa gtgacattta ttttctttga tggaggaaaa
    44341 gaagcaaatc ggataactgc gggtaaggga ggaataactg attttgataa ggagttgatc
    44401 cctgaactga atcgacttcc gttagccaat atcaaacaat tagaagaaga attggataga
    44461 acgggagtaa cttcccaaaa ttccgagatt gctaattgag acattggcgc gatgaaacgg
    44521 tgggtctata tagcgctatt tttaattatc agaacaagtg atgtttgatc ttcaaaataa
    44581 caaataaaaa attaaaaaat atagttgacg aaattcaaaa attcgattag acttaaatca
    44641 attcttaagg aaagggtgac ctgtaatgaa cttatcaaaa cgtatttcag cagaaaacaa
    44701 aaaaaattgg cgcagctggc gtagataagt tgtattaatg gtgagacttt cccatagata
    44761 caacttttta gagtgcattt ctaaagcttg tatctatgct agacatgaac attgactgcc
    44821 gcatagatta gaatctatgc ggttttttgt gtaaagacaa tgaccgcttt ggccattgtc
    44881 tttttttgta gactggatac ttgcctgatt ataataaata aaaagattga ctttgcagga
    44941 ggaaaaaaat gaaaaacaaa cgattaataa cagttgtagt aattatttta gtttatttag
    45001 caggtgcatt tatttatgga aaagcctcag gcgaaaaaga aagtacttcg acgaaccaga
    45061 caaaaaaaac aacagttgtt ggggtattgc aatacgtgag ccatccggca ttagatgaga
    45121 tttatcgagg aatcaaagat ggtctgaaag aagaaggatt aaaagaagga gaaaatcttg
    45181 agatcaaatt tcaaaatgga caagccgatc agagcaagct agccacaatg agtcaacagc
    45241 tagtacaatc tgatccagat gccttgattg gaatcgcaac acctgccgct caatcattag
    45301 caaacacgac ttcatcgatc ccagttgtgt taggtgctgt aacggatcct gtgggggctg
    45361 gattagtcgc ttctttagag aaacctggtg gtaatgtaac aggtgtttct gatcagccgc
    45421 ctgttacttc acaaatcaag cttgcttctg atttgttgcc aaatgcaaaa aaagtcggaa
    45481 tcttgtatgc ttcttcagag gataattcaa aataccaagt agcacaagca gaagaagctg
    45541 caaaaaaaga agggttagaa tcagtaaaat atgcggtgcc ttccactaat gagattgcac
    45601 aaacggttca agtgatgagc cgacaagtcg attttatcta tgttccatta gataatacca
    45661 ttgccaatgc gatgcaaaca gtggtaaaag aagcaaacaa agcaaatatc cccgtcattc
    45721 catctgtgga tacaatggtg gaacaaggtg gactagcaac aatcgggatc aatcaatatc
    45781 aattagggct tcaaagcggt aagatggcgg cagaacttgc gagtggaaaa gaaaaaccgg
    45841 aaacgacgcc tgtttataca tttgatcaag gggatacagt gatcaatcaa tctcaagctg
    45901 atcgcttagg aatcattgtt ccccaatcga tgaaggaaaa agccaaaatc attacagatg
    45961 actcgcagca agaaacatct aaggaggatg acaaatgatc gtatcagcta tttcacaagg
    46021 catgctttgg gcaatattag gattagggat tttcatgact tatcggatct tagactttcc
    46081 tgatatgacg acagaagggt ccttcccatt aggtgctgct gtctgtgtga cagctattac
    46141 tcatggggta gcgccgattg tagctacatt attaggcgta ggtgctggca tgatagcagg
    46201 actcgttacg gggttacttt atacaaaagg aaagataccg gttattttag cagggatttt
    46261 agtgatgtca ggattgaact cagttatttt gtttgtgatg cgaacaccaa atctttcttt
    46321 attgaatcat ccgattctac aagacagctt ccatcgcttt gggttacctg attactttga
    46381 cacagtcctt cttggcttca tctctttggg catcttgatt gctgtactgc tattcttctt
    46441 caatacagat ttgggtcaag gctatattgc gacaggagac aacgaaacga tggctcggtc
    46501 gttagggata aaaaccgatc gtatgaaaat tttaggttta actttgtcga acggtgtcat
    46561 tgcattatcc ggtgcgctga ttgcccagaa cgacggatat gcagatgtaa ataaagggat
    46621 tggcgtaatc gtcatcggtc ttgcttccat catcatcggt gaagtgatct tccaagaatt
    46681 gacgctagca gaaagattga ttgctattgt tgtcggcagt atcatttatc agctattgat
    46741 tttagtagta atcaaactag ggtttgacac gacttatctg aagttgttct ccgctatcat
    46801 cttagctatt tgtctgatga tcccgcaatt gaagaaagca ctgaacttaa aaacgggttt
    46861 tgagaaggag gcatagtaat gactgatata ttgacaatca aaaatggcag aaaagtcgta
    46921 aacaatggaa tgaatgaaga gaaagtacta ttggatgaaa ttaaccttac aatcagagaa
    46981 ggggacttca tcactgtcct tggcgggaat ggtgctggaa aaagcacact gttcaatacg
    47041 atcgctggta cgcttcaact cagtcaagga tcgatctctt ttaaagatca gctgatcaca
    47101 aaagattcag aagaaaaaag agcacgattc ctttctcgag ttttccaaga ccccaagatg
    47161 ggaacggctc ctcggatgac agtagcagaa aatttgctac ttgcacaaaa aagaggtcaa
    47221 aaacggaaat tccgtttacg taacttgaaa aatcaaaaag aagatttttt cagactatgt
    47281 caagaagtcg gtaatggcct agagcaacac ttggatactc cagcgggtga attatctggt
    47341 ggtcaacgcc aagcattaag cttgctgatg gcgacgatca cagctcctga gttactatta
    47401 ttagatgaac atactgcagc tttagatcct aaaacagcta aagcattaat gcgattaaca
    47461 aatcagcgga tcaatgaaca aaaattgact tgtctcatga tcacgcatcg aatggaagac
    47521 gccttgaaat acggaaatcg tctgatcgta ctccaaaaag ggaaaatcgt tcaagatatt
    47581 tctgaaaaag aaaagcaaca tttgacaatg ggcgaccttt tgcagttctt tgaagaagta
    47641 gtggattgat ttcgacagaa aaattccttt cagtagaagg acttgaaaac tcattgacat
    47701 cttcctttga ttcatcctat acttaaaaca ataaacaatt aaacatattt gggtgttttg
    47761 taggatcacg tagtcaatga ttttaacaat taaatagaat attctttgct ctgacaagta
    47821 aacgatgaga aaagaatttg ctactttgcc acctagacgt ttcgtacttt ttcgtatcaa
    47881 acgctaagtg gcttttttta ttgaagtaag aacaaaggag gaataagatg atttctggac
    47941 aaaccaaatt agctgggctc tttgctgatc cggcttctca cagtttatcc ccattgatgc
    48001 acaatactgc ttttgaagca aacaacatcg atgcggtgta tttagctttc caagtaaatc
    48061 caatgaattt gaagcaagca gtcgaaagta ttcgaacttt tgatatgtta ggtgtaaatc
    48121 tttcgatgcc gaataaaaca gcggttattc cttatctgga tgaggtaagc caggaagcgc
    48181 aattgatcgg agcagtgaat acgatcgtgc acgaaaaggg tcgtttgatt ggctataata
    48241 ctgatggtgc gggcttcatg caatccgtgc aagaagctgg gatttcgatc aaaaagaaaa
    48301 aaatgactct actgggagct ggaggtgcag caaaggcagt cactgttcaa gcagcgttag
    48361 acggcgctga ggaaatcgta gtttataaac gaaacaacca aacattcgct caagtaaaag
    48421 aaacattcga acaactcagc tatcgaactt cttgtaagat aaaagttcgt gactttgccg
    48481 atacgcatca attaagaaaa gacttgaaag aaagctgtct tctagtcaat gcaacagata
    48541 tcgggatggg aaagaaaatc aatcagtcac cgctcttaga tataagcatg atgcatgaga
    48601 atctagcggt ttttgatttg atttatgcac ctagagaaac acgtttatta aaagaagcaa
    48661 aaaaagtagg agcaaaagga tacaatggac ttggcatgtt gatctaccaa ggagcaatcg
    48721 cgtttgaatt atggacaaag gaaaagatgc cgatccatgt gattgaaaag ctgtttgatt
    48781 aaaaaaggat aatagataaa gatcttttat tctcgcataa acggtctcaa gaaaagtaat
    48841 cctttatgta tcgaagcatt tatttcgtta ttttttatta aattatcaaa aaatgatagg
    48901 cttttgtcga ttggttatcg tatcctttat ttatcagtaa gaaacgagga ggaaccacaa
    48961 tgagtcggat ggatcgctat aaggacatcc ataagaaggc gaaacctctg aagcgggata
    49021 cggggagtat ttttgctaga agagaacgaa aacgaacgcg tatggaaaat gaggaagatg
    49081 ctacccgtat ttatgaaaga gagcagcatt catttgaaca tttgaaccaa tacgagcaac
    49141 agatatacag agatgcacca agagcagaag aacaacaaaa aaataaaaaa tttttcaatc
    49201 ggcaaaagaa gaacaaaccg ttaaaacctc gaagaaagcg atcgtggaaa aaaatcattc
    49261 tatcgatttt gcttgttttg atcctttatt caggtatctc attttttatt ggtaaatatg
    49321 tagctgaaca tgacacaagc ttagaggcag cgacttcaga aacctttcat gggcaaaaag
    49381 cgacgaatgg tgcgcataat attttgatcc ttggaagtga tacacgcgga gaagatgccg
    49441 ggcgagccga tacgatcatg gtcttacagt tggatggtcc ttcccataag ccgaaactga
    49501 tttcttttat gcgtgacagc ttcgttacga tcccaggggt aggacaaaat aagatcaatt
    49561 cagcttatgc atacggagga gccgatctag tgcgtcaaac tttagtagaa aattttggaa
    49621 tcgattgtca atactatgcg aaagtcgatt tcaaatcgtt tgaaaaagtg atcgatgcct
    49681 tattcatgaa cggagtgaag atcgatgcag aaaaagatct gaatctggat ggtgtagata
    49741 ttaaaaaagg gggccagaag atggatggac atgttctttt gcagtacgca cgtttccgaa
    49801 tggatgaaca aggagacttt ggccgtgtca gacgccaaca gcaagtaatg aacgctattt
    49861 tcagccaatt gaagaatcca ttgaatctga tccgtgcacc ttatgcagct ggtaaagctt
    49921 taggatatac atccacagat gtcactgctt ggttcttgac gaaaaacctc ttttcgattg
    49981 cacgaggagc tggtggcatt gatcgaataa gtgtgccagt agacggttcg tggaattatg
    50041 ggcaaagtga ttatgctggc agtattcttg aaattgataa tcaaatgaat caatctgcta
    50101 ttacagattt tttgaataaa tgatcataaa aaagatcaag aagaagctgc gcaaggcttt
    50161 tgtcatgaat caatcaaacg attcactcgt aaaaaacgct ttcttatggt agatttaacc
    50221 aagagaggag ctgtttacat tggatatcta tctgaacttc aaaaatgagt cgcgtgaagc
    50281 catcgctttt tatgaaaaag tttttcagac tgaagcatca ggtatcacca catacggtga
    50341 aatgcctgga atagaacaaa tgaacttgcc agaagaagca aaaaatctaa tcacgaatgc
    50401 acggctgaac atttatgata cattagttat gtttgcagat acgccagaag gaattggtcc
    50461 tgaattagtt gtgggaaata atgtttcttt agtcatccag acaagaaatg aagaagaaac
    50521 gaagcgccta tttgaaagct tagctgaggg tggtaagatt gagatgccac tggatgctgt
    50581 tccgtgggct cagttgtatg ggatggtaac tgatcgattt ggcatcaact ggcagattaa
    50641 ccaagagtga cagctaaaga tgaatatttt ctggaaacat caagcagcaa acggatcatc
    50701 gtttgtttgc ttgatgtttt tgtgtctatt tcgactatac ggtatctttt ttttattgcc
    50761 agatgcgaaa atatgttcca taatagagaa gtccgcaaaa aattaagtaa cggaggtcag
    50821 aagatgaaga cggtgatact agctgaaaaa ccttcacaag caaaagccta tgccgaaacc
    50881 ttccaacagc aaaagcggca tgaagggtat tatgaaatca gcgatccatt gtttgctggt
    50941 gaagtaacca tcacttatgg ttttggacat ttggtagata tggttgctcc aggagcttat
    51001 gaagagcgtt gggcaaaatg gtctttagaa aatttgccta ttttcccaga aaccttccgt
    51061 tacgaagttc caatggacaa acgtgcacaa ttttcgatcg taaaacgaga attgcagtca
    51121 gccgatacca tcattattgc aacagatggt gatcgagaag gtgaagccat cgcgtggtcg
    51181 attattcgac aagcaaacgc attttctaag gaaaagaact atttacgttt atggatcaat
    51241 tctcttgaaa aagaagcaat ctatgaaggt tttaagaatc tccaacctgg aaagaactat
    51301 tttccaaaat ataaagaagc gcaagcaagg cagaacgctg attggctgat tggaatgaat
    51361 ggaagtccat tatatagctt gctacttcaa cgaaaaggga ttgatggcag tttttctctt
    51421 ggtcgtgtac aaacaccgac actctatatg atttatcagc tgcaagaaga aatcaaacac
    51481 ttcaaaaaag aaacgtactt tgaaggagaa ggtttaatta cgacaaacaa agggcagttt
    51541 tctggaaaaa tcgtaccaaa aaaagcattt aaaacccaag aagagttaac gaaggaaata
    51601 gaaaaacttg gagcgcattt aggtaagcaa aacggacgga ttcttgaagt tacgaaaaag
    51661 gaaaagaggt tgcatagtcc acgtttattt tcgctatcta gtttgcagac aaagatgaat
    51721 cagctaatga aagcaagtgc caaagctaca cttgatgctg ctcaaggatt gtacgaaagc
    51781 aagtttctaa gctatccaag aacggattcc ttttacatta cagaaaatga gcatcagtat
    51841 ctggttaaac atctgcaaaa ttataaaaaa tttttaggaa tagaagaagt acagacaact
    51901 gagatcaaag caaagaaacg atatgtcgat gcgaaaaaag ttcaagaaca tcatgcaatt
    51961 attccgacaa agacgattcc gactcctgca cgctttgcga agctttccaa attacagcaa
    52021 gcaatctatt tgcaagtttt gcgcactact gtggctatgt ttgctgcaga ttatatctac
    52081 gaagagacga ccgtaatgac gggcgtacag caattaaagc taaaaagcat cggaaagatc
    52141 ccattacagc aaggttggca ggaaatctta cgcatcaagt caaaaaagaa agaagctcag
    52201 actcttcctc ctatcgtaga aaatgaagaa gcagaagtag aactgaaaag tatggagaaa
    52261 gaaacacaac ctccaaaacc ctataccgaa ggaacgttga tcacagcaat gaaaactgcg
    52321 ggaaaaacgc tggataatga tgaagcgcaa gaaattctca aagaagtaga aggaataggg
    52381 acagaagcta cacgagccaa tatcattgaa aatcttaagc agaagcatta catcgaagtc
    52441 aataaaaacg aaataactgt tacgccaaaa ggaatcactc tttgtaaggc agtagccaat
    52501 gaaccattac tgacgagcgc agaaatgact gcgcgctggg aagggtactt gagaaaaatt
    52561 ggtaaacaag aggggacacc atctgttttt ttagagaata tcaaaaaatt catcctacat
    52621 ttattggcag aagttcctaa tcaaatcgac aaggtagact tttcagatga gattagcggg
    52681 acacgtcaga taaaagcgat ggagaagaaa aacgatcaat taggaagatg tccaaaatgt
    52741 aaaaagggaa tagtcatgct ttatcctaaa atcgctacat gcttgaatcc ggaatgcgac
    52801 ttcaaattgt ggccaacagt agctaaaaag aagctgacta aaacgaatct cagagatttg
    52861 ctttcaaaag gaaagacaag taaagttgtc aaaggtttta ctggtaaaaa agggaaattc
    52921 gatgcagttt tagtattaaa agaagatatg acaatcggtt tttcttttcc atggatcgaa
    52981 aacagtggaa caaaagcagt tgatggaaac aaagaaacat caagaaaaaa taatgaaaac
    53041 gaagtagagt aaatgaaaac aatgaaatag cggaatattt attccgaaaa gtgagaatat
    53101 aaataccaat aaaactaaaa aatatcagtg aatcaagcaa aaacgttggg gtaggctgat
    53161 tttatataga tagcaaggag tttttttatt tgaaaaaagt gcattcattt ttaaattatc
    53221 ttatgggagc aatcacgatt gtggctgtgt accaagcatt tctaaagtat cctgattacg
    53281 ggacgaagtt gttgtcattg acgcacaatc aaacagcata ttcaccaacg ttgattttat
    53341 cgactttgac tgcaggagct aaaagcgcta cttatttttt tcataaaacg acaagcatgc
    53401 taaagcgaaa gaaacagcga aaacaattgt tggtggtggt agcggctatg atcattattt
    53461 attttctacc agttattaca caagtatttc atcaatattt tggttaagaa tacgagagtt
    53521 tgtgacaaaa gtcatgtcac aaactttttt ctttgtcatt ttttcgtaag aaaaagcaaa
    53581 ataaaagtat atacattgat taaaagtata tacttggata taatgtactt actatagaat
    53641 gagaatggta tagagggtgg cgaaatatga aaaaaatgaa aaagtataaa attagaaaat
    53701 ctggcaatag cgatgtaaca actatcccac cagaagtaaa agaattcatg ggtgtgcaga
    53761 caggtgacgc aatcagttat gtttttcaat ctgatggttc tgtacgaatg atcaaagcac
    53821 aagaagaacc tgatattgat tcattagttg attcgatcat gaatcaatat gaagacgcgt
    53881 taaaagattt ggttgattta tgaggtatct ttcgactaaa gaaattatca aaatcaacgc
    53941 gaaagttatt ttacgtcact cgccaggcga aatgatcggt gtaaaagatg caaatgcatt
    54001 agatatggcc gtcaaacagc cttcacaagc agtattcaat caagaactct atcctgaaat
    54061 ctatgaaaaa gcggcgattt tagcaattaa tttagctaaa aaacatcctt ttcataatgg
    54121 caataaacga actgctcttg ttgccatgtt gttgtttctt caattgaata attgtccagt
    54181 tgctttttct cgtcaagaag ctgttgattt tatcataatg attaccacta gttcattgga
    54241 tttcgattca ctaaagtcca agataaccag ttatttacga caaaccgcaa taaaatgaac
    54301 gaattaaaaa ctttatttct caattagatc agcatgtcat taaggaatat gctgattttt
    54361 ttatgtttaa tcatacaaaa tagttttttc ataaaaatat ggaaaatatg gattaatttt
    54421 gaaataattg atttaaaagg aatacaaaag agtgaatagt aggaaatagt ggtttagaac
    54481 tagtgggctg attttttata tattttagtt tacataatat atattataca aagtagattt
    54541 aaaaaatgat aaacctctct cgaacggttt tttatcaaaa atcgtcattt ttccatcaaa
    54601 atctgcttat gcaaaattat aaaaaaattt tgcagttttt cttacttttt ttgtcgttta
    54661 attgaatgat aaaaaccagt ttctttattc agttaatcaa atatgaacga attagatatg
    54721 aatcattgta tttgccgttg cggaaggatt ttttaattaa ccacagataa acattgataa
    54781 cgattcgctt attgtaatca acagattaaa tttcatatgt tcatatgtac ggatacattt
    54841 tcggaccgat taattttaac cattctgttt atttgcttac ctattagata tcaataaaaa
    54901 actgcttaac agctatttca gctgttaagc agttttagga tttttagttc atattcattg
    54961 tttcttataa tacagtgttc aatacttctc ttaaagcact tgttgatttg ctgaacattg
    55021 ctttttcttc ttcgttgatt gataattcaa caacttcacg aacaccattt tcgtcaacga
    55081 ttgatggcac acctgtaaag atatcttttt cgtcgtattc accagttaaa taagcagata
    55141 caggcaatac agcttgttcg ttattcaaga tagcttttac gatacgtgca gtactcatac
    55201 caataccata gtaagttgct tttttacgat caatgatctc ataagcagca ttacggactt
    55261 tatcagcaat cacatctaat tcatcttggg caatacgatg gtctttttct acaatttcaa
    55321 acactggttt accaccgaca gttgtatgtg accatgcagc aacttctgaa tcaccatgtt
    55381 cacctaagat atagccgtgg acgctgcgtg ggtcaacttt taatttcaat gcgatttctt
    55441 tacggaaacg agttgtatcc aatgttgttc cagtaccgat aacacgtgat gttggcagac
    55501 cagattcgtt ccaagcgata tatgtcaaaa tatctactgg gtttgaagca acaacgataa
    55561 taccgtcgaa tcctgatccc atgatttctt ttacgatttg gcgcatgatt gacgcatttg
    55621 ttttaactaa atctaaacga gattgaccag gtttttggtt gataccagct gtgataacaa
    55681 cgatatcagc atctttacat tcttcatagc cacctgacca tacagccaca ttttcatcgc
    55741 cccaagccat accatctaaa aggtccaatg cttccccttc tgctttttct tgattcacat
    55801 cgatcaatac taattcatta gagattcctt ggttgatcat tgcataagcg atacttgttc
    55861 caacaaaacc tgtaccgacg attactactt tacgacttgt ttttttcatg aataaacagc
    55921 tccttttcat aactatttgt tactttcatg aaaagtatag tcttgtttat tcattttatc
    55981 aacatgttaa ttataatagt tgttattata atttttgatg gaaacgaatt cctgaatacg
    56041 cataacagat aaatgacaaa tgtttacacc tatatcccct gataatacaa ccaaaagttt
    56101 agaactgaaa ataaatccaa aaaaattcgc tgcaacgata taataagaca gcaatcgcac
    56161 ctaaaacaat tttccttcat atgctttagt gaaagatttt cttcttaatg tttatttctc
    56221 cattcattgg gagtacatgt tgaatacttc ttgaataatt tgaataaaga agtcgtttgt
    56281 ttatatccga tacgattagc gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc
    56341 aacgcaaagt cgaatttttt ctttagaacc agttcttgaa aggaagcatc aaaatacatt
    56401 ttcaatttat tactaagata gaccggattg tagccaaaat gagctgctgt ttcttttaac
    56461 gtacaagaag taaaatgctt atcgatataa tctaatactc taagcatctc attttctgcg
    56521 catccatctg ctgatgaaaa gctttgttgt tctaataaat gagtcaattc gatcagtaat
    56581 agtgatgtat agagatttaa cgaacggatc ttatgactgt atgtatcaaa ataaatcaac
    56641 agcatatttt caatcaaatg cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat
    56701 tgtgtatgtt cccctttttc ttttgatgca tttatcaaaa aagaagaaag tagattattt
    56761 gtgcttgcca aatgattcaa tatctctgta gataaggttt cttcttttaa taagatgttg
    56821 attaaaatat cctcttgccc tgtgtattcg attctttgag tagcttcttt atcaatgatc
    56881 accacgtcac tttcttccat ctctatcctt tctccattaa taatttgtaa acatgtaccg
    56941 ctatacatat aattgatttc aacaaaatca tgtgtatgct cgggaacttc cgcatatcga
    57001 cggtgtttac tgatatgaat atttccctgt tgaaatagat cccctcggtg gatttttggc
    57061 ggtgtctgaa gatggtaagc taagtttaaa tcattgatat tcattccttg ttcacgctgc
    57121 agagtttcga ttgattcttc gttacgcaaa aaaaatgtct aattcagcta gttccaattg
    57181 actccctcct ttttgtaagc ggtttctttc gcttattagt tcacgctgta ctgctattat
    57241 atgcaaagct atttttggtt tcataaagac tttctatggt tgggacatga acttcctgtg
    57301 attccgctat tgagagaata accacttcat actatatttt tgataagcgc ttacaaaaat
    57361 gaaaggaaca tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc agtctctctt
    57421 attttgactt ctgctggtgc agtcacgtct gcaattccca gtatgtcagc tacttatgcc
    57481 acgcgttctt tatctgctgt tgaattatta acgactgctc cctcttttat ggtcactatt
    57541 tttgttttga ttagtagttt tattgctagg aaaatcggac aaaaacaaac tgtgatcatc
    57601 ggattagtga ttgcatcgat ttgtggtatc ctccccctct tctcacaaag ttacagtatt
    57661 gtcttagctt caagagctgg actaggtatt ggctttggat tgatcaattc tttggcagtc
    57721 agcatgattg ctgcctttta tcacggagat gaacgagcct ctatgattgg ttttcaaagt
    57781 gcatttcaag gatttggtgc agctttaatg acatttgttg caggacagct agtacagttc
    57841 ggttggcaat ataccttttt agtctacgcc atcagcttac ctattcttgt cttgtttgtt
    57901 ttgtttgttc ctacacctga aacaactgaa cttcaatcgc atggcacagc cccaacaaga
    57961 caagtaatca aaacgaatcc taaaatgatt agttatggta tttttctctt gctggtcatc
    58021 gtggtatata atgctgtatc gattaaatta gccacagtcc tgacgactta ttctctagga
    58081 aacgaaacta atgcagcgaa tatcttaaca atcatgcaat tggcaagtat ggctgccgga
    58141 gtttgttttg gctggcttca gacaaaagca catcgctata tgcttacact ttctttacta
    58201 ctgatgggaa tcggctttgt attgattgcc actgtgataa acctgtatgt gattgggttt
    58261 ggtgcgatcc taacaggaat ttctttttcg ttattcattc cttacttatt caaccaagta
    58321 agtatccaag cgcccaagta cgcggagagt ttcaatactt caatcttatt ggttggcgct
    58381 aatttaggta ctttcattgc accatatggt ttgctctttc tctcttttgc ttctcttggg
    58441 agtaaaacac ttgctgtttt cgtcaatggc agtatcttgc tgttgattag cgcgcttatt
    58501 agccttctag tagtacacag gacgaataga aagacagtga taagagtaga tgaagaaata
    58561 aaagaacaat tgacaacaaa ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa
    58621 cataaagata gatggctttg gtatcctgga gactttgaga tccgccatgg actgctacaa
    58681 aatttccaac gggaagaacg aggttttgac tggccagcgt actggtacat ggatgattgc
    58741 catcggaacg tcaagtttaa gagatattac tttcttgatc aaccttccat gtttaaagtg
    58801 acgatccaag gagttgggta tgtagaaatt aatggacaaa aacatccatg tggaaaatgg
    58861 ctgacttgtc cagctggtaa agcaaaaatt cgtatctttg taggacatac atcagggcta
    58921 ccagcgatgt ttatcgaggg agacgaagta aaatcagata ttggctggac agcaagtaat
    58981 tttattgaag aatatccagc tggctggagt ccgctttatg tcgatatcgc aaaagatcca
    59041 aatcaaatct attatcaaaa agaatgcgtc catccagtca ctgaaacgga gattggcgga
    59101 ggaatcttat tcgattttgg gcgagcagtg aatggctgtg tagaactaat atctctggat
    59161 gaccatatcg aatctatcac tctatgttat ggagagtccg atgtagaagc tatggatatc
    59221 gattattgct actataagca aataggaatg acaagaaaaa tgaaagctcg taaacgcgct
    59281 tttcgctatt tgtttatacc agaggtttct actggaatgg ttcaacttca agtttttcat
    59341 gaatttttat ctctttcgaa acaagctgct ttttcaagcg attcttcttt actgaatcag
    59401 atctggtcag tatcagaaga agccttcaag ttatgtagtg atttgttttt tatcgacggt
    59461 atcaaacgag atcgctggat ttggtctgga gatgcgtatc aatcattcat gatcaatcaa
    59521 tatctttttt ttgacgagga aatcaataaa cgaacgatat tagcattaag aggacaaaat
    59581 gaaatcaagc agcatatgaa tactatcgtt gactattcga tcttgtggtt gattggtatc
    59641 gaaaaccact atatgatgac gaaagactat gaatttttac gcatcgtcta ccctaagatg
    59701 caatcattaa tgagatactt gatggagcaa acgaatgaat tagggtttat ttatgggaga
    59761 gcaaaagatt ggatatttat tgattggtct gaaatggata aagaaggaac gcttgctgca
    59821 gaacaagtct tattactgaa agcctatcat tccatgatca cttgcggaga tatcttagct
    59881 gaaacatatg gagagaacga aaattatcat actcatctct atcaagaaaa atatcaacta
    59941 ttgaaacaaa acctatttga ctacttttgg gatgaagaac ttggcgcttt tatcgattgc
    60001 tatgaatctg ggagaagaaa tgtgacgcgt catgcgaata ttttcgctgt tttattcgat
    60061 tttgctaaca aagaccaaca gcaatcgatc ttaaagaatg tcttattgaa tgacactatc
    60121 acacagatta ctactccata tttcaaattc tatgaacaag atgctttatg caaactcgga
    60181 cagacgaata tcgtctacca aacgattctt gattattggg gagggatgtt atctcatgat
    60241 gcggtaactt tctgggaaga atacgaccct actcaaacag gaaatgaaaa gtacagtatg
    60301 tatggagatc cttatggaaa aagcttatgc catgcatggg gagcaagccc aatctatctt
    60361 ttaggacgct attttatcgg tttgtatcca acaaagccag gttatgaaga atttatgata
    60421 caacctaatc tagattcatt tgaaacactg aattgtacgc tacctatcaa agatggaaag
    60481 atttcgcttg aaaaaacaaa agataagatc gtgattactt ctgatagaac aggcggaaca
    60541 gtgaaaatga atggtaaaag ttatcctctt ctagcaaaac agccacttgt cttaacaagc
    60601 tattcaaaaa taaatgcata aaacaaaaaa ctgcttgcga catatcagaa gactttaaat
    60661 cttcttctga tattgtccaa gcaattttta ttttgaagtt tgcttaattc tgttctttag
    60721 aaacaatgtt tgttcttttt ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga
    60781 aagcgatgat tccaactgtc aaggctattt gaccgtattc tcctagtttc ccaagtacaa
    60841 gtcctggtac tagataatca gtatcggcaa atgtagaaga agcgatttta agatctccta
    60901 atactggaag gataaataat ggcagccaac taatcaataa gctattgaaa gcagaaccta
    60961 ataccgcccc tcttctaccg ccagtcgagt tgccgaagac accagaagca gctccgcaga
    61021 agaaatgtcc tactacccca ggaatgataa ctgtagtttg caagcctatc atagcaaaca
    61081 tggatagtgt tcctacaaag aagctaacaa aaaagccaat taatacagca ttaggtgcat
    61141 atggaaaaac gataggaaca tctaatgctg gtttagaatc gggtacgagt tttttagcaa
    61201 tcccttggaa tgctggaacg atttctgcca acaccattcg aaccccttga aggacgataa
    61261 caaacccagc tgtgaatgtt ccagcttgga tcagtgcaaa gattacagcg ttgtctccat
    61321 tgcttagttc tctttcaaca aatccaggac cagctaacaa agcaagtgcg acatatacga
    61381 tcgacatcaa gagcatgatc gatacagtag aatctcgcaa aaagctgaaa cttttaggaa
    61441 tctttatgtc ctctgtagaa cggtctttgt ttccaaaaaa ctttccgatc catccactag
    61501 aagcataacc gatatttccg gtatgcccta acgcaacttg attgtttcca gtgatgttct
    61561 tcataaacgg ttgacaaaga gcaggcgtaa tagttaatag tgttccttcg aagaccccgc
    61621 ctaataagat catccacaaa tttgcagtaa accccgcgct tactaaaatg actgctgtta
    61681 gacaagaaac ataaagcatt gcttgacctg taagaaaaat gaatttatat cttgtgaatc
    61741 tagcaagtaa aatattcacg atcatcccta ctagcatgat caaagcagta ggtgtaccgt
    61801 attgttgcaa agcaatcgcc actactgctt cattgctagg aactactcct tgagtatgca
    61861 gagctgtttg gaacatcgaa gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc
    61921 cgatcagtac taggaatccg acaaatgttt tgatagttcc ttggatcact tctgaaaatt
    61981 tcttcttctg aagaacgaga ccaagcagtg caatcaaaga aacgaggatg gagggctgac
    62041 tgacgattga cacaaacaaa ttcaacaatt tatccattga tagttcctcc cttgaatcat
    62101 ttttattctt ttgacaagat cttcgttact ttttctttta cttcttcgct gtcaatgatc
    62161 gattgcaaag gtacgagctt ttcatcaggt atagaagtta gcgtatcttt caaagaatgc
    62221 tcaatgaaga aataatcagc catatcgcta gatacacttc caacatccgt atgacttact
    62281 tcaaactcgt ccacatctgc tccaagatct gcaagtgctt gtttgatgtt catctctacc
    62341 ataaaacttg ttcctaatcc actttgacat actgcaaaaa ttttcatatc attcgtctcc
    62401 ttttcttatg atcgctaata tttcttcttt tgttgatgcg tgtaataaat cttgaagatt
    62461 ttgtttatta gaaagtagct ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc
    62521 gatagcagct aaacagatga acaaactaat gggatgggct ggattatcaa gcagatccac
    62581 aggttgttga agtttcagca aagacatccc tacttcgttt acgccatctt ctggacgagc
    62641 atgaggtaat gcgatgtgat cgccaatatg gataaacgca ccatactgct ccacgcgctc
    62701 gatcatagct ttgatataac tttcttctat tttattttct gataatagcg gctgtgcggc
    62761 aaatgtgatt gcttcttgcc agttttttgg ttgatccgat aattggatca tttcttctgt
    62821 taataattca gatagcatag gacgttgatc ctccttcttt tctaaaagtt tgttcatttt
    62881 acgatttaat acttgataga gcttttcttc gtctacgcct tcttttaatt cgatataagg
    62941 cagtagcgcc gacaacaatt cttgggcaga aggcattgaa aatcctggta tcagccaatc
    63001 tctttgtact tgattgatca gctgattttt ttccaagtca gtcatgattg gatgcagaac
    63061 atagagagac tttttcttat cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta
    63121 agaagtttcg tcaacctcgt gtaagcggtc agctgaatta gtctctgtaa aaaagatcat
    63181 cgggaataat tgctgaagtt cactttttaa gatcaatgaa gagctaatgc cacttggaca
    63241 gacgatcacg gctcttattt ttctatttct ttcttctgcg tccgcttttc tgatctctcc
    63301 gccaaatagt atagtgaaaa aaccaatctc ttcttcaggg atgctcttcc ctattcgtct
    63361 ttctaacgga agtaatgctt ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat
    63421 ttgatcgatc atcacattag gtaaataaaa agaataggaa atacgaaaat aggcagggac
    63481 caagtgataa aagacattca tcaataattc acgaaacgaa gcaaattcta cggccatcag
    63541 gaattccatg cgatggatga cttctgaaga acattccagc aaaaaatcaa gtgcaggctc
    63601 ctggacatct ccttcaacga tcgatgctaa gcaaagagag aaaaatataa tctccgaatt
    63661 ttgatgttcg gaatagccaa ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag
    63721 agaagccaac agttcagcat cttttaccag ttccatggat gtttttccaa ttcgaatggt
    63781 tgtcaaactc aaataataag ctaattcatc cattcgattt ggtgcgaatc gaagatctga
    63841 tagtgaaatc gttttatcta gcaaatctct aactttggcg taataatcaa tggaaagggt
    63901 tgcggttaaa tacgtgagcc caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg
    63961 attttttgct tctcttctta atattgcttc tgaccctagc agcctaaaac cagttttcct
    64021 agtatacgac agctgacaat cttccacttc taattcattt cttatctttt tgatatctga
    64081 caggactgtc cctttagaaa cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt
    64141 tgcagtcgaa taaaccatca aataaagaag atctcttcgc tcttgttcgg tatacacagc
    64201 ttcaaatcga gtaatatcca aaaatttttc tgcccagtcg attgctcgga atttctttat
    64261 acggatacgg gtatcatcca catattcggc aaaaggagtt aattgtttga tcgcctcgct
    64321 tgtatttact tcgatgatct ctgctgcttt ttctagactg atttctcttg gtgttttcag
    64381 gagataacta attttgtagt tcacagcgct gttcacctcc tacttatatt ctagtatggt
    64441 agcgctttta aatcgagggg tgtttggtac aacttacagt tggatttttt ttacgactgt
    64501 accgaacgat ttctatcgaa tggatacaga aaaaaagcag gtgaccaaat gtctcctgct
    64561 tttatgaatt gatttcgtcc gatcaatgat gatcatgttt attttttgag gtcagttctt
    64621 caaatttttc tttgacatct tcaaccgctt tttcgatatc tttttcaaag tgattgggct
    64681 cattaggctt tgtttcctct tctacagaat ttgaaccaga taaagctgtt tctttatttt
    64741 ctgtttcacc agagtttgtg ccagctaatg attcttcttt gtcttctgtt tcaccagaat
    64801 tagttcctgc taaagccact tcttcttctg ttttaggggc cattgaacct cttaatcgtt
    64861 tgtgtttttc accgtcataa tttgtcatgt tggttcctcc tagtagttct atactttttc
    64921 atcgtacaat aagacagttt atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat
    64981 atttttcctt ctgattcttg aatgcttttg attccttctt ccaaaatttt catttgtaag
    65041 attgtttctt catctttgat gactttttct gcgccattaa ctaatgtctc gtaaatccct
    65101 tcgtagacac ggctgtaatc accaacctcg gaaattactt tttcttcatg ataactccct
    65161 tgttcatcta tataggtcaa tacaccataa tgttcgggca aatcgatacc aaagtcttca
    65221 tgatcaggca tgtaaaacat tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg
    65281 aacatccctt tttttccgta aacgacgaaa cttggacgtt ctttgatgcg gaaataactg
    65341 gattttatcg aaattttcat atttccatag aaaaaatcca gatcgaagta atcattcatt
    65401 cgtcctttcc ctaaaagttg acgtacatca tagctgatat catctggttc gccgaaataa
    65461 gaaagaactt gatctactgt atgacaagca tgtccatata aatagctgtt cgcagtagaa
    65521 aattcatgcg tattttctgg tacttccggt ctgaaataat cataatgcat ttctacttct
    65581 aaaatatccc ctaattttcc gctttcgatt actttttgga ctgtcaagaa gtcagaatcg
    65641 aaccgtctgt tttggtaaca ttggataaat agaccttttt cttttgctaa tgcaaataac
    65701 tcttctgctt cctttgacgt ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt
    65761 tcgagtgcca tttttccata ttcataatga aattgactag gtgtagtgat gacaactaat
    65821 tggatctcag gatcatccca aagatcagac agctgatcag tatattcgat gtcttcgaat
    65881 gttttccaca ctggttcaga tggggaaaaa atttttttga tattgatttt gttcgtaagt
    65941 tttcttgaaa aaggcaaatg atagcgattt gtactctttc cattgccaag ataagcgatt
    66001 gtaagcatga gtggtgcctc cttttattga tgttttcagt atatcctaga aaagcgcaga
    66061 aagtatttat aatagctatt ctggtaaaaa agttcagaaa aacacttgtt atcattcggt
    66121 ttggtgaaat taatgttcaa agtaaggata aaaatcaaaa agatagtagc ggaaccaagt
    66181 gtttatcagt aagatagaat ttggaggtca gatcatgctt attgaagaaa aactaacaaa
    66241 acaagagttg tttaccacga cagagaaacg gattgtcgat tacatccgta ggaatatcga
    66301 agctgcagtc tacatgacga ttgaagaatt agcaaaagct acgtatactt cccattcagc
    66361 tatcattcgt ttatgcaaaa aaatgggatt cagcggattt aaagaattcc gtttcgagct
    66421 ggggcgggaa gttcatcaat tgattgctca gttcaatcaa acgattgatc ctaattttcc
    66481 tttttcaagt gaagatagca ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca
    66541 gtcaataaaa aaagcacaat tgcagattga acatgaagat ttagatcaaa tcggtaaagt
    66601 attgaacaag gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa tcacggcaag
    66661 gaaattccag aataaaatgg tcaagttgaa taagtttttg atcatggctg aagaatacag
    66721 tgattcttct tggaatgcag ccaatttgac tagcgaggat tgcgccattt ttattagcta
    66781 cagcggtcgc atccatcatt atgaacgaat aatgacctat ttgaagcatg tagaggcccc
    66841 taccttgctg attaccggaa atcagcattc agaaatggcc aaacaggctg atatgtgtct
    66901 tgttatatcg caggaggaat atgattttgc taaagtagcg actttctcct ctcaaattgc
    66961 cttcgattac gtattaaata cgttattctc agtgatatat tctcaaaatt ttgaagagaa
    67021 tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta aagaatatcg
    67081 gaaaacttaa gaaaagatcc tcatttagta ataagaaaga tagtgtttga taggctctct
    67141 ttgatatcat cataaaaaca ggctgaagtt ttctttttca aacctcagcc tgtttgttta
    67201 cggaacgatt cgaatagata tattatagaa atgaggaata gctaacttct gtttcttatt
    67261 ttgaacgatt gacgaaaaag atatataaga aggaggaaaa tcagtataca tgcagctaac
    67321 aatccataca ggaaaaatcg tttgttctcg gatactttct gtacttcttt ttgaacttct
    67381 tttgcatgat aaggaacccg gtgccctctg acaagtaagc gatgactgtt gaccatataa
    67441 ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt cgattctcag atcttttgta
    67501 tcagtcggtt ctaccgtctt gatctgatcg acttgataag cgagtgtccg tccattgatt
    67561 tcgatataga aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga
    67621 ggcagtcctc gatggctgga aataacggca tgtgtatttt ctccccccgt aggataagac
    67681 gttccttcca ataatgaggc acctttttct agcagcacag tattcgtttc atcaaaaatt
    67741 ggcaaatgga cattgatctc agggatcgtc aaaataccga tcgtatgttt ctcaaaatag
    67801 gttctatcct ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc tggatttcca
    67861 ccttcttttg ctaattgctg gtttttcttt gtcatgtttt cttggatttt agccatgact
    67921 tcttcatttt ctttgaccgc ttgttgctga taatgagaga tgatctgctg atccagataa
    67981 ttattcagcg cgtcgctaac aaatggataa gctagtatcc ctattcctga gagaagcaag
    68041 aaaagcatca gcagatctag aagtttcctt ttctttggtt tcatttgtgt tctccttgtg
    68101 atgactgttt gataataaaa gaaaaaggag catttgcgta atccgctgct cctttttcca
    68161 attcttgcaa aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat
    68221 cagtagaagg actactcctg caccgatata cacatagatt cccttaccgc ctgttgaagg
    68281 aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc ctgctgttac
    68341 ataagattgt tcatcgatag taaatgctac acggtttgtt aatggaacat atttttctgg
    68401 cgctttcgtt tcttccagat agtacgtgcc atattttaga cctgtcacat cgattaaacc
    68461 gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc tgacggcttt
    68521 tgtggatggg tcgatcgata aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa
    68581 ttctgctcca gcaagtgttt ggtctgatgt aacgtcacca tctactttaa caaatcgttt
    68641 gcccccagta acgacatcga ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac
    68701 atttgcttgg ttagaaaatc ctttggttgg atctgctttt tcgttcaaat gcatatagta
    68761 aacgaatttc aatgtaccgc caggagttaa tgaaggaata taattcggat caactgaaac
    68821 cgtgaatccg tctgtttgct cagtgacaga ataatttact gggtcgattt ctttatttcc
    68881 atcatataag gcataagcat acgttcctga agaatcatta tcaaatgtta aagcagcgtc
    68941 atgagtatcg ataagtttga atgttgtgta cttgttttgc gttccttctt tatcagcgat
    69001 acctaatggg atattgacag aaatctcata ttgaattttt tcaccgattg cgacatcttt
    69061 tccattcaat tgaggtgttg ttttatcaac tttagacgta tcatttttga tggtcttttc
    69121 tactggtgtt tggctatttg ccaggatctc aaaaggcgtg attgtttgct tttcaatcat
    69181 ttccgcatta tcaggagctt ttacttcttc taaattatat tttccaactt ctaaatgatt
    69241 aacgatcaac tggccttttt caatagatgc ctcggcaaag tcattgttgc cgatgtcata
    69301 agaatgacca gtaatgaaat gtttggcttt ggtttgatca gttgtccaag tgtacaatcc
    69361 atctgtgaca ctttggatgt attttttgac gcttggtgtt ccttcttctt tagaaataat
    69421 aaattctgct ccatttagtg cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt
    69481 tccatcatta ccgactgtat ttttagggta gagatggata gtatctagtt cttctgtccc
    69541 gtatttataa gagccatctg cttgtttgat catctcatat acagggaaag ctaaaaccat
    69601 gtttgcggca gctgacactc cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt
    69661 cccattttgt tttttaggta aagataaagt gacatttcca tcagcatctg tcgttcctga
    69721 agcaactggt gtaccaggag tcaaagactg gactgcttgt tttgctgcat ccacggacgc
    69781 tcctttatct cgttgcgcat aaaattcttg agtgacgtta taaactgaaa atgaaatatc
    69841 ggctaatcct tggtattgat cgaattcgct catttctttc ccgctgtttt ggattaaagg
    69901 atcgggtaaa tcagtcattt tctttttatg aaggatgact tgagctgctg tctcctctgc
    69961 aaacactttt tttgcgccga agctgtttgt gagtaatggt aaaataagga aaaggactcc
    70021 taacataacg tttatttttt tatgattttt cattttttca ctcctgttct ttttttgata
    70081 aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc
    70141 gtttcgtttg tttttggaag aaatggacgg ctattttctt ttttattgac tactttcaat
    70201 atactgtccg cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt
    70261 tcacttaaaa tataaccaga aggtgcttcg atctctttta atcgataact tcctgttttc
    70321 aacccggaaa ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc atctgattcg
    70381 ttctttgtcc acttgtatcc gttggctgtt tgctggaggt aatttcctgc tttgttttta
    70441 ataaggaata ctgcgccagc taatcgttta tctggtttat ttgtctcgat tttgacaaat
    70501 tgcttactcc ctgtatatac agagactttc tttgtactga caccaaaacc aaaatccagt
    70561 gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg catctgataa
    70621 gaaatggtca gtttttttcc agcatatttt tccaacgaag ggatcgaaaa taaaacatca
    70681 aatccgtgag gtttcttgtg tatgacaaag ccttctgtca gcttttctcc attcgatgaa
    70741 atcgttaaac tattaggtaa aaacgttaat gcttcatccg cactatctga caattcgaac
    70801 ttttgatagt caaggatatt tacaggaatt gtcgtatgca aggaataagt gattttttca
    70861 ccaatcgtaa aatcatttct aggctcttct aataccttct caaaaggtgg atcagggtag
    70921 tcgttttctt catttttagg atacaaatgg atagttgaaa gtttttgatt gttttgtcca
    70981 tgtacaggaa gaacaactac catattctct gccttttctt tgacgacttc tggtactttg
    71041 gattcaatga aaagatacgc tttatctctt ttttcttgat ctttagcggc cagagaaaaa
    71101 gaagcgatac catcttcgtt gttaagagtt gtagttgttg cttctgcggt aaacataccg
    71161 gaagacgcac cgttttttgc gatctctgct tgtgcttctt ctaccgtttt gcccttttcc
    71221 cgtagatggt aaaaagaatc tgtgacatca taaacttgga atgtgacacc atttaatcct
    71281 cgatacgttt gtaataaagc tttttcttgt ccgtcatttg gatgattttt cggcagttgc
    71341 ccgtttggga aaagcaattt gtgtaaagta atctgaacca tttctgtttc tgtttctgtt
    71401 gctacctgag taaaagctgg tttaaatagt aacaagaaga gacacatact aagccaacca
    71461 agttttttca tatttagctg ccccctcttt cttttttcaa aaaaagatac caaatagaaa
    71521 acgcacaacc aatcatccct actagataga ttcctaaacg gccaattcct cccgtttcag
    71581 gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg
    71641 gtgacagaac agattcatga tcttgtccat ccacttgaat tgacccatct tcgtgtataa
    71701 ctatagtaac tggctctttt agaccttggt atccttctgg tgtaaaagtt tcggtcagcg
    71761 tataagttcc aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg
    71821 gcgattctaa gtccatttct ggtccctgca gtctgaattt tgctcctttt aatgtctgtc
    71881 cgttgtcttc ttttttgtgt accgttagat caaacgcttt tagtcgattt tgatgagtga
    71941 gcgtccatac attatttgtc atctcggtat tttttcccgt gtaaatgatt tttccatatt
    72001 tatcgatttg gaattcataa tttcccggcg aagtatcata tccggcagga ccagctgttt
    72061 caacgactcg atatttacca ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg
    72121 ttgtttggct gtcaattggc tgataagtac catttgcttc ttttttctgt agggaaaatg
    72181 ctgctccttt aagatttatc tctttgtccg tcccttgcat cgcatactta cgtactgcta
    72241 caggaacttc aacaaaagga ttttcaatag tcaactggat cgtatcatcg gaagtagtga
    72301 ctgtttttcc atcaatggtt accgtaccat cagaagcgat cttgatttcc caagtagtct
    72361 ttttgcttaa tccatgccct tctggagctt ttgtttctgt cagcgtatag gtcatttctt
    72421 tttgcaattt gacattttct ggaagagaat aggttccgtc gccatgatct gttagtaaag
    72481 tatcaataga atctcctgtt aatttgaaaa cagcgccaat aagatccttt tcacctgtag
    72541 aggaattttt cgttattttc aagtttaacg gtgtgaattg tttagtattt ttccatgtca
    72601 ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac
    72661 tgaatgcttg accttgatta ttgtattgag gtagtgataa tatctcttga taactttctc
    72721 cgctatttgc agataatttg tcaatgtctg cacgttccca cgtatttgtt gtatctttag
    72781 ctggtttaat gattcgaata tatccgtttt tccaagctgc agcatttgtc gtatgttccc
    72841 gttgaatctc aaaagtaact tgatctggac gatcagctag attgttgtca aattcttccc
    72901 ataacttttt gatgtgaaga ctgactccgg gagctttagc agatggtata ccgaattcag
    72961 ctaattcatt ggtatcaatg tttggctgga aagttgtcct gccgttcatt tgataccaga
    73021 aatttggatg gaagtcctca ttttctgttt ggattctcac ttggtaatgg atttggattt
    73081 cttggtcttt tcctaaatag atctgattgc tcttgatggt atttccttct atggaaatag
    73141 atggagtgac cgttgtagga cttgtcccca cactcttgac tgaaagtgta ccaggctgat
    73201 aaatgaatgg ttctgcgatt ggatcattta tttgtccatt gcttacagtt gctgagatct
    73261 gtacagcttt tttggctagg tattcagaga tatctgttgc atgatcagct gattcatagt
    73321 aaagatcgcc tttttcatct gatgaaacca tttgtcgcat acgagactct acttctgctt
    73381 ttgagagacc agctgccgga tcgctttgaa gttggatgcc aagaccatgt atttcgattc
    73441 ctcgttcttt gagtgccatc gcttctccga tggttgcgat aaacgtactg tcgattcttc
    73501 tggatagatt gttttggtca ggtgcatcat agattcttga gattagagaa gtatttcccg
    73561 gccgatcttg cgtattagaa aactgagttc cgtaataatt gctgcttgat tgtgcgtgta
    73621 cccgctgtac tttataggaa aatgttggta caccatccgt cagcaaaacg atcacttttt
    73681 tatgtccatt tggaacggat agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag
    73741 taccaccatt tgtccgtgaa ggtgtaatgg attttacttg atttttcact gaatcaaatg
    73801 aacccatctg tactgcaccg ttactgtagc tatatccttc gcttgagtag ccgacatatc
    73861 ccatattgat tttgtctgtg ataccgctat ctgctaaagt atcgacaaaa cgatcgacac
    73921 caatctttac ttcaccgatc cgattattgt cgttcatact tcctgaccag tctacgacca
    73981 atacgagatc aagaggcgtg atatcttttt gtacatttcc acgagcattc aaataaacat
    74041 caaacaatcc gggtgtactt gtttcctttg catactttcg tatcgcatag tctgcttgat
    74101 taccggttcc tccatattcg atatacgaat gggtccgatc attaggatct ccattccaag
    74161 aatttatacc atcccattga tttgttccgt ttttattgcc ttgatgatca agaacatttg
    74221 tatttcctgt aggttgccaa ctggctttag gataagtgcc ctgatcatct gttgtatatt
    74281 ctgtaggaat cgtttgatta gatggcacac tcacagctgg caaggataat gtaggcaatg
    74341 taaatgggct gacagaattt tcgttttctt ttgatgattc ttcttctatt tcgtctggct
    74401 ttggtactga cacatctgta tctttcttat tttctgtctc atcaataatg tcatattctt
    74461 tattatcgac gatcagcttg tttgcctggc tgtggtaaag tgctggatcg attggaaaac
    74521 tcagatcgat agtttgttta gtgtttgatg agatttccaa cgtaattttc ccgctattta
    74581 cctgataagc atagccatct ccttcttttc tttccacatc acttaagtaa ttttcgacta
    74641 gaccaatttc tttttctatc ttttcagcac tggtgttatt cgcatttagc ttgatgttca
    74701 gtgctttttt tccagactga tccgaaatga cttcagctga aattgtttct aaaactgttg
    74761 gatcatctgt agtgtctgct attgcagata atggcacaaa gtttgataaa ctcaatatta
    74821 tcagcatgaa aataactttc agtttcttcc ctgtggtcgt catttttccc ctcctttttc
    74881 tttttttctg ttttgacgtg aatagtgaaa attattaaaa taaacaggcg ttacataatt
    74941 ttatttattt ta
//
LOCUS       DACEMK010000002        70985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEMK010000002 DACEMK010000000
VERSION     DACEMK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.002"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            336..1439
                     /locus_tag="IW715_000068"
     CDS             336..1439
                     /locus_tag="IW715_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1020703.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IW715_000069"
     CDS             1723..2403
                     /locus_tag="IW715_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1020704.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IW715_000070"
     CDS             2493..3527
                     /locus_tag="IW715_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1020705.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IW715_000071"
     CDS             3541..3876
                     /locus_tag="IW715_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020706.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IW715_000072"
     CDS             3860..6340
                     /locus_tag="IW715_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020707.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IW715_000073"
     CDS             6396..7955
                     /locus_tag="IW715_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1020708.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IW715_000074"
     CDS             7952..9316
                     /locus_tag="IW715_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1020709.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IW715_000075"
     CDS             9487..10557
                     /locus_tag="IW715_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1020710.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IW715_000076"
     CDS             10572..11240
                     /locus_tag="IW715_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1020711.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IW715_000077"
     CDS             11260..11838
                     /locus_tag="IW715_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1020712.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IW715_000078"
     CDS             12241..13152
                     /locus_tag="IW715_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1020713.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IW715_000079"
     CDS             complement(13408..14325)
                     /locus_tag="IW715_000079"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1020714.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IW715_000080"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IW715_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1020715.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15570
                     /locus_tag="IW715_000081"
     CDS             15022..15570
                     /locus_tag="IW715_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1020716.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15685..18045
                     /locus_tag="IW715_000082"
     CDS             15685..18045
                     /locus_tag="IW715_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1020717.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18132..18446
                     /gene="trxA"
                     /locus_tag="IW715_000083"
     CDS             18132..18446
                     /gene="trxA"
                     /locus_tag="IW715_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1020718.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18628..20406
                     /gene="uvrC"
                     /locus_tag="IW715_000084"
     CDS             18628..20406
                     /gene="uvrC"
                     /locus_tag="IW715_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1020719.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20551..21450
                     /locus_tag="IW715_000085"
     CDS             20551..21450
                     /locus_tag="IW715_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1020720.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21443..22705
                     /locus_tag="IW715_000086"
     CDS             21443..22705
                     /locus_tag="IW715_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1020721.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22868..24766
                     /locus_tag="IW715_000087"
     CDS             22868..24766
                     /locus_tag="IW715_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1020722.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25065..25784)
                     /locus_tag="IW715_000088"
     CDS             complement(25065..25784)
                     /locus_tag="IW715_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1020723.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQELPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26121..26453
                     /locus_tag="IW715_000089"
     CDS             26121..26453
                     /locus_tag="IW715_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020724.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26440..28443
                     /locus_tag="IW715_000090"
     CDS             26440..28443
                     /locus_tag="IW715_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1020725.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28462..28932
                     /locus_tag="IW715_000091"
     CDS             28462..28932
                     /locus_tag="IW715_000091"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1020726.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28953..29537
                     /locus_tag="IW715_000092"
     CDS             28953..29537
                     /locus_tag="IW715_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1020727.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29552..30538
                     /locus_tag="IW715_000093"
     CDS             29552..30538
                     /locus_tag="IW715_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1020728.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30528..30839
                     /locus_tag="IW715_000094"
     CDS             30528..30839
                     /locus_tag="IW715_000094"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1020729.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30861..32642
                     /locus_tag="IW715_000095"
     CDS             30861..32642
                     /locus_tag="IW715_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1020730.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32635..34011
                     /locus_tag="IW715_000096"
     CDS             32635..34011
                     /locus_tag="IW715_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1020731.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34018..34653
                     /locus_tag="IW715_000097"
     CDS             34018..34653
                     /locus_tag="IW715_000097"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1020732.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34683..36038
                     /locus_tag="IW715_000098"
     CDS             34683..36038
                     /locus_tag="IW715_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1020733.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36230..37624)
                     /locus_tag="IW715_000099"
     CDS             complement(36230..37624)
                     /locus_tag="IW715_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1020734.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37716..38687
                     /locus_tag="IW715_000100"
     CDS             37716..38687
                     /locus_tag="IW715_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1020735.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38684..39466
                     /locus_tag="IW715_000101"
     CDS             38684..39466
                     /locus_tag="IW715_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1020736.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39635..40006)
                     /locus_tag="IW715_000102"
     CDS             complement(39635..40006)
                     /locus_tag="IW715_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1020737.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40140..40583
                     /locus_tag="IW715_000103"
     CDS             40140..40583
                     /locus_tag="IW715_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1020738.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40626..41435)
                     /gene="yidA"
                     /locus_tag="IW715_000104"
     CDS             complement(40626..41435)
                     /gene="yidA"
                     /locus_tag="IW715_000104"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1020739.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41454..42821)
                     /locus_tag="IW715_000105"
     CDS             complement(41454..42821)
                     /locus_tag="IW715_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1020740.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42805..43635)
                     /locus_tag="IW715_000106"
     CDS             complement(42805..43635)
                     /locus_tag="IW715_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1020741.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43798..44235
                     /locus_tag="IW715_000107"
     CDS             43798..44235
                     /locus_tag="IW715_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1020742.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44311..44931
                     /locus_tag="IW715_000108"
     CDS             44311..44931
                     /locus_tag="IW715_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1020743.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45120..45626
                     /locus_tag="IW715_000109"
     CDS             45120..45626
                     /locus_tag="IW715_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1020744.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45643..46542
                     /locus_tag="IW715_000110"
     CDS             45643..46542
                     /locus_tag="IW715_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1020745.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46629..47513
                     /locus_tag="IW715_000111"
     CDS             46629..47513
                     /locus_tag="IW715_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1020746.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47705..47776)
                     /locus_tag="IW715_000112"
     tRNA            complement(47705..47776)
                     /locus_tag="IW715_000112"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47741..47743),aa:Arg,seq:ccg)
     gene            47836..49170
                     /gene="rpoN"
                     /locus_tag="IW715_000113"
     CDS             47836..49170
                     /gene="rpoN"
                     /locus_tag="IW715_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1020747.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49373..50410
                     /locus_tag="IW715_000114"
     CDS             49373..50410
                     /locus_tag="IW715_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1020748.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50451..51452
                     /gene="gap"
                     /locus_tag="IW715_000115"
     CDS             50451..51452
                     /gene="gap"
                     /locus_tag="IW715_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1020749.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51562..52752
                     /locus_tag="IW715_000116"
     CDS             51562..52752
                     /locus_tag="IW715_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1020750.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52856..53611
                     /locus_tag="IW715_000117"
     CDS             52856..53611
                     /locus_tag="IW715_000117"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1020751.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53727..55025
                     /gene="eno"
                     /locus_tag="IW715_000118"
     CDS             53727..55025
                     /gene="eno"
                     /locus_tag="IW715_000118"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1020752.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55425..56564
                     /locus_tag="IW715_000119"
     CDS             55425..56564
                     /locus_tag="IW715_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1020753.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(56631..57206)
                     /locus_tag="IW715_000120"
     CDS             complement(56631..57206)
                     /locus_tag="IW715_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1020754.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(57211..58185)
                     /locus_tag="IW715_000121"
     CDS             complement(57211..58185)
                     /locus_tag="IW715_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1020755.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(58209..58253)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            58468..59436
                     /locus_tag="IW715_000122"
     CDS             58468..59436
                     /locus_tag="IW715_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1020756.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            59549..59827
                     /locus_tag="IW715_000123"
     CDS             59549..59827
                     /locus_tag="IW715_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1020757.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            59945..60181
                     /gene="secG"
                     /locus_tag="IW715_000124"
     CDS             59945..60181
                     /gene="secG"
                     /locus_tag="IW715_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1020758.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            60293..61039
                     /locus_tag="IW715_000125"
     CDS             60293..61039
                     /locus_tag="IW715_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1020759.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            61042..63399
                     /gene="rnr"
                     /locus_tag="IW715_000126"
     CDS             61042..63399
                     /gene="rnr"
                     /locus_tag="IW715_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1020760.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            63421..63885
                     /gene="smpB"
                     /locus_tag="IW715_000127"
     CDS             63421..63885
                     /gene="smpB"
                     /locus_tag="IW715_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1020761.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            64187..64906
                     /gene="atpB"
                     /locus_tag="IW715_000128"
     CDS             64187..64906
                     /gene="atpB"
                     /locus_tag="IW715_000128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1020762.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            64959..65174
                     /gene="atpE"
                     /locus_tag="IW715_000129"
     CDS             64959..65174
                     /gene="atpE"
                     /locus_tag="IW715_000129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1020763.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            65265..65789
                     /gene="atpF"
                     /locus_tag="IW715_000130"
     CDS             65265..65789
                     /gene="atpF"
                     /locus_tag="IW715_000130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1020764.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            65776..66318
                     /locus_tag="IW715_000131"
     CDS             65776..66318
                     /locus_tag="IW715_000131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1020765.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            66341..67897
                     /locus_tag="IW715_000132"
     CDS             66341..67897
                     /locus_tag="IW715_000132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1020766.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            67911..68813
                     /locus_tag="IW715_000133"
     CDS             67911..68813
                     /locus_tag="IW715_000133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1020767.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            68836..70242
                     /gene="atpD"
                     /locus_tag="IW715_000134"
     CDS             68836..70242
                     /gene="atpD"
                     /locus_tag="IW715_000134"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1020768.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            70256..70678
                     /locus_tag="IW715_000135"
     CDS             70256..70678
                     /locus_tag="IW715_000135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1020769.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        23555 a        12433 c        15089 g        19908 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccaatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtgatgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    15481 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    15541 gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt
    15601 ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt
    15661 catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa
    15721 aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca
    15781 gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga aactgaggat
    15841 ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga aaatatccgt
    15901 ccccatatga aacggatcga gatcggagca gacttgaatg gagtggagct tgcacaagtt
    15961 tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttctgacagt
    16021 gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg
    16081 agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct
    16141 gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta
    16201 gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga
    16261 aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc
    16321 cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt ggtggatttg
    16381 aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt
    16441 gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc ttatgtgatt
    16501 ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agccatcgtg
    16561 ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat
    16621 caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt
    16681 acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg
    16741 atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag
    16801 gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct
    16861 tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta
    16921 tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc
    16981 ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg
    17041 aaagtttatg gatacaatcg ggcaaatacg attaatgcca gcatggagtt cgatgtggat
    17101 acactaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa
    17161 atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat
    17221 gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca
    17281 gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat
    17341 ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag
    17401 aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat
    17461 attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat
    17521 gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg
    17581 aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac
    17641 ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata
    17701 ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca
    17761 caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac
    17821 cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc
    17881 attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc
    17941 aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg
    18001 gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta
    18061 agcagatggt tcgctatcca agtatagaaa tgctatacta atcatagatt ttaaaaatgg
    18121 aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag
    18181 gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga
    18241 ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg
    18301 tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt
    18361 tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac
    18421 gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact
    18481 gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt
    18541 tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc tataataact
    18601 ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta
    18661 cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc
    18721 ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggcag ccataatacg
    18781 aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat
    18841 atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt
    18901 atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt
    18961 ttggtgatta ctcgtaaagt actaaaagac aaagcactgt attttggacc atatcccgat
    19021 gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc
    19081 aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct
    19141 tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaagttt
    19201 ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct
    19261 gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca
    19321 atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat
    19381 gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt
    19441 gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac
    19501 ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat
    19561 acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga
    19621 ggagaaaaga aaaaattagt tgaattggcg aataagaatg cctcagttgc tttgaatgaa
    19681 cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga
    19741 aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg
    19801 gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa
    19861 tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa
    19921 gtcatctatc gcagatattc acgagtgatt cgcgaagggt tgcctttacc tgatttgatc
    19981 ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta
    20041 ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg
    20101 ctattcggta tagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta
    20161 ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc
    20221 agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa
    20281 aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa
    20341 ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag
    20401 gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa
    20461 aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata
    20521 tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact
    20581 tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg
    20641 gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt
    20701 ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat
    20761 gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac
    20821 caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc
    20881 gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg
    20941 tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg
    21001 atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat
    21061 ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat
    21121 aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat
    21181 ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt
    21241 ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac
    21301 aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg
    21361 actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc
    21421 aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt
    21481 aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg
    21541 tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt
    21601 attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga
    21661 gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga
    21721 tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa
    21781 ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat
    21841 gcttcgagca agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc
    21901 aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag ttggagaaga
    21961 taactcgaca gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt tcattattat
    22021 tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat
    22081 ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaac taacaggtgt
    22141 tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag
    22201 tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt
    22261 gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc
    22321 tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat
    22381 tttgccagtg acttatctat ctctaggcgg atatatgctt ggattgatac taggagcttc
    22441 agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat
    22501 tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt
    22561 ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa
    22621 tgtactcgtg tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat
    22681 gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat agaaggaagc tggacattta
    22741 ttgttcccag cttctttttt gtatagacct atttctattt ttttattata ttggatatat
    22801 atattcattg aaaatagagc ataacaactt tataatgttg agtataccaa tttttggagg
    22861 cgttttaatg gaccgctatt tagatttact ttcagagaag ttccctacaa cggaagatgt
    22921 catcacggaa atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt
    22981 tttgagcgat atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg
    23041 aaatcttaaa gaaaagatca aagacctttt ctctgaacaa atgacggaaa cggagatcag
    23101 ccaattcact atttttacgg cttatccaga atatgtgttg acgactgatt ggtacaaaaa
    23161 acaagataaa tcccagctga tccatcgttt gatcgatctt ttacggttta cgacagtcaa
    23221 atacactcga tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga
    23281 actcttgtat atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga
    23341 acaacttgtc ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc agacgatcca
    23401 aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt tttgatcgag gaactcaagc
    23461 agccaaagta atggatcgaa tcatggattt ttcctctgta gatatccagt gggggaatca
    23521 tgatattttg tggattggtg cgtattgcgg ctctgaggca tgtttattga cgcttttacg
    23581 gattgccgca cgctacaact atttatttga attagagaaa gaatatggat taaatttacg
    23641 tccattattt tcttttgccc atcaaaccta tcagaagaat ccagtattta cccctaaaaa
    23701 caggaaagat ctatctgaaa gagagcaaga agaattagaa aagatccatc aagcactttc
    23761 tatcctccaa ttcaaatcag aacatcagtt gttgaatcga cgacctgaat ttaaaatgga
    23821 cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca acgatcgttt tagaaggtca
    23881 gctctatgag ttgaaaggga cttgttttca aacgatccag ccagatgatc ctaaaaaatt
    23941 cttggaagag gaacaaaaag tgatcgatag tttgatgtat tcattccagc actcgttaag
    24001 aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca
    24061 gcatgtactg ttccatggct gtatacctgt agatgaaaag gggcagctct tagcatttga
    24121 attagaccaa tcttatcgcg gaaaagaact gctgggattt tttgaaaaac agataactga
    24181 aagcgcaaaa gatttgactg caaatgaaga tttggcgacc gatttgattt ggtatgcgtg
    24241 gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg agcgctattt
    24301 tctcacagag cctcatacgc acgtcgaaaa aagcaatccc tattatcact tgcgagatga
    24361 agagtggttc agtgaaaaaa ttttggcaga attcgatgca aaagaagaag gttcagcaat
    24421 catcaatgga cacacgccag taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg
    24481 aaaagcattt gttattgacg gaggtctatc caaagcttat caaaaaacaa ccgggattgc
    24541 tggctactca ttactatgca attcttacgg tttccaaatc gtgacacatt atccgttctt
    24601 gcctattgaa caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga
    24661 gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca
    24721 gattactcaa ctccaaaggc tgttggatta tcgaaaggac gattgagaaa aatgctagcc
    24781 aacattgctc aatagactaa aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg
    24841 aattagctca aaaaacggta aaatattaga agctattttt ctgtttattt ctctaatact
    24901 caggtcaggg cgctgatttc acgacctctt ccttaaacgg tgttctcctg ccaactcctc
    24961 ggtcacaagt cacaatatcc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
    25021 actcagagtt gaacggcagg ctcacgacct aatcagaaaa acgattaaaa ttcattcaca
    25081 aagaaatcga ataaacgctg ttcagatgaa tctacacgat gagaaagttc cggatgccat
    25141 tggacaccaa ggattttgtg catattatct ttactttgga ctgcttcaat gagtccatcg
    25201 ttggaaaagg cgatggcttt taacaaagga gaaagatctt tgactgcctg atgatgatag
    25261 gaattcacaa agtatgaatc agggagaagg tcacttaata aactgtccga aacgattttg
    25321 acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac ggaccatttt
    25381 ggatataatg aaagatcttg ataaagtgtt ccctccaaca cgacattcaa tagttgaagt
    25441 cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg cttccaagat caatgcctgt
    25501 tcgaattggt ctcgttgaat atttgtttcc gtcagttttg gatgagggtc ttccatgtaa
    25561 agttgcggac taacatcctg accgcctgca agaatgagtt tgtcgatttt ctcaattagt
    25621 tgaggcgctg aatctggaga catgactggc agaataagag gtaattcttg agcatctaaa
    25681 actgcatcca caaatccctg cggtgtatag cttacttgat ttccttggaa agtatctgtc
    25741 gctcgaatca gttgattccc ggcaatgccg atgattggtt tcatttttat tccccctaaa
    25801 gtagactaat tgtaacaagg atttaaaaaa atttaaacta tgtaagactt gcccctatac
    25861 ttatctgttc ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa ttcatgacga
    25921 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    25981 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    26041 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    26101 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    26161 cagaagaaga aaatgagaag cgtgctcgaa ccctacggca gcagttgaag acctatgcag
    26221 aagaaaaaga gcaagaattg gagataatcc aaacgaacag tcggcaacag ctgaaacagt
    26281 tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga
    26341 aagcagaagc agacaaggtg caagcaactt tgaaagacca atatcaaaag catcagagtc
    26401 aagcaatcgg tgcaatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    26461 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    26521 atccaaaatg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtagaa
    26581 aaatattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggcaat
    26641 cggctgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    26701 aagaaactcc atttgaaaca tcaagaactt tccttacaag acttggaagc aaattattcc
    26761 gaagaaaatt tcatgaataa attagaagaa gtccttgatt taaaagaaaa gtgggaacag
    26821 ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    26881 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    26941 agtgcagcaa attttgaaag tttcaaggaa gaactaagta agcttgatga cgtttatatg
    27001 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    27061 gttgctgcta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    27121 gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc aattatccat tgttcacgag
    27181 cagcaaaaaa agataacgac agcgttaggt cagtgcagcg gctatatcca agaatttgaa
    27241 tgggtagaag aagtcacact tgccgttgca gaacgggaaa aagtaaaaga acggtttatc
    27301 catgccactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    27361 atgcatgtgt taaacgaaac tgtatcagaa gaagatatat acgttagttt cgaaacacca
    27421 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttatcgaa
    27481 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaacg
    27541 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttac
    27601 ggattgctga tgctcattgg gacaatacta gctcaaaaac tatttgtttt accccgcggg
    27661 atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac catcgatcat ctggggattg
    27721 atctacagtt catttttcgg acaggcatta ccaaaagaac tatttggtat ccgcttgcct
    27781 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    27841 ggtctgatac agatcttggt tggattattc gtggcagcaa aggaaaatat caggaggaaa
    27901 gattacttga gtgctgtcag tgatggtttt gcctggcaag ggatcctgat tgggatcgtt
    27961 attgcattga ttggtgtcat gttgataaaa aatacagcat ttgtttatct tggcgcgggg
    28021 ctatcgatca ttgctgcttt atgtatctta atcgtgccga ttatccaatc gccgtctaaa
    28081 gtcaagggtg ctgccaaagg agcgtataat ctttacggat tgactggtta tatcggtgat
    28141 ttagtcagtt atacacgttt gatggcacta ggtatttcag gcggaagtat tggcgcggca
    28201 ttcaatatgc tagttgcttt catgccgcca gccgctcgat tcagtgtagg gatccttttg
    28261 atcatcgctt tacacgcact gaatatgttt ttgacactgt taagtgccta tgtccatggt
    28321 gcacgattgc agtatgtcga attttttgga aaattttata caggaggcgg acgtgccttt
    28381 gacccgctga aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa
    28441 taattggagg atttttatac aatgatggat tacttaatta gtcaaaatgg tggaatggta
    28501 tttgcagtat tagcgatggc aacagcgacg attttttcag ggattggttc agctaaaggt
    28561 gttggaatga caggtgaggc agcagcagcg ttaacaacaa gtcaacccga aaaattcgga
    28621 caggctttga ttctgcaatt gcttccaggt acgcaagggt tgtatggatt cgttatcgcc
    28681 tttttgattt ttatcaactt aggaaatgat atgtcagttg tacaaggatt gaactttcta
    28741 ggcgcttctc tgccgattgc ttttacaggt ttgttttcag gtatcgccca agggaaagtt
    28801 gcagcagcag gtatccaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    28861 tttgctgcga tggttgaaac atatgcgatt ttaggtttcg ttatttcctt cctattagta
    28921 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    28981 agatgaatga agcagcagag caagaaagag cggcgttaga gcaagaagag cgaatgaaga
    29041 tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag
    29101 aaaaacaaat cgaattactt gaaaaaaaat accgtcagct taggaatcgc caacaagtag
    29161 aagtacgaca agaaaatttg aatgcgaagc aggaattttt acgacgattg tttgcagatg
    29221 caatagctga aatggagaat tgggatgaat ctgaacaaat ccagtttatc aaaaattcat
    29281 tgtattcatt acctttgaca ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt
    29341 atctttctca gacattgctg gatgaatgga acaatgaact tccctttatg atggttttaa
    29401 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    29461 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    29521 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    29581 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    29641 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    29701 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    29761 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gctatacttt
    29821 ccataatctg aaggttttga caaaagccga gatgacaaat caaaatctgg accatttgta
    29881 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    29941 aaatgaactc cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    30001 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    30061 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    30121 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    30181 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    30241 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    30301 ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt gaacagttga aagatgatta
    30361 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    30421 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    30481 aagccatttc gaaccagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    30541 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    30601 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    30661 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    30721 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    30781 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    30841 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    30901 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    30961 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    31021 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    31081 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    31141 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    31201 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    31261 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    31321 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    31381 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    31441 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    31501 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    31561 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    31621 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    31681 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    31741 atacatcaaa catgccagtg gcagctcgag aagcttctat ctatacggga atcacaattg
    31801 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    31861 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    31921 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    31981 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    32041 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    32101 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    32161 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    32221 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    32281 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    32341 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    32401 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    32461 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    32521 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    32581 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    32641 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    32701 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    32761 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    32821 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    32881 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    32941 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    33001 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    33061 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    33121 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    33181 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    33241 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    33301 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    33361 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    33421 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    33481 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    33541 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    33601 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    33661 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    33721 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    33781 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    33841 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    33901 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    33961 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    34021 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    34081 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    34141 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    34201 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    34261 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    34321 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    34381 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    34441 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    34501 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    34561 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    34621 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    34681 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    34741 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    34801 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    34861 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    34921 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    34981 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    35041 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtta
    35101 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    35161 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    35221 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    35281 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    35341 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    35401 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    35461 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    35521 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    35581 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    35641 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    35701 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    35761 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    35821 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    35881 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    35941 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    36001 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    36061 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    36121 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    36181 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    36241 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    36301 aggataggga aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat
    36361 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    36421 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    36481 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    36541 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    36601 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    36661 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    36721 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    36781 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    36841 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    36901 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    36961 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    37021 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    37081 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    37141 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    37201 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    37261 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    37321 cttcattggt agagatggtg atggactcaa agacattggg agaattaaat gtgccacttc
    37381 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    37441 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    37501 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    37561 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    37621 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    37681 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    37741 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    37801 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    37861 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    37921 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    37981 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    38041 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    38101 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    38161 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    38221 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    38281 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    38341 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    38401 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    38461 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    38521 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    38581 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    38641 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    38701 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    38761 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    38821 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    38881 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    38941 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    39001 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    39061 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    39121 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    39181 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    39241 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    39301 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    39361 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    39421 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    39481 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    39541 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    39601 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    39661 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    39721 cagtaatctc aagaccggca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    39781 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    39841 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    39901 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    39961 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    40021 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    40081 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    40141 tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    40201 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    40261 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    40321 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    40381 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    40441 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    40501 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    40561 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
    40621 atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa
    40681 gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga
    40741 ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct
    40801 tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta
    40861 tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt
    40921 tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg
    40981 acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact
    41041 aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg
    41101 tcatcgatcg aatggagatg actgccgaca cggcgtgcca tcacttcgat ctctaggaaa
    41161 tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt
    41221 gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt
    41281 tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca
    41341 gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta
    41401 ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat attttaattt
    41461 atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat
    41521 aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca
    41581 tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc
    41641 aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga
    41701 taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat
    41761 ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa
    41821 aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata
    41881 ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg
    41941 aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt
    42001 tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa
    42061 attacttcca taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg
    42121 ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca
    42181 taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg
    42241 aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt
    42301 tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa
    42361 ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct
    42421 acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca
    42481 tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag
    42541 cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt
    42601 gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg
    42661 aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc
    42721 aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact
    42781 ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt
    42841 attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt
    42901 tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc
    42961 aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc
    43021 cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga
    43081 ttgataaaat cgcttcatga cttgcccgcg tttttcctga ttaccgttga cacttaagta
    43141 catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc
    43201 atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat
    43261 cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat
    43321 ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc
    43381 tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg catctttcag
    43441 tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt
    43501 gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa
    43561 tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg
    43621 aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta caatattata
    43681 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
    43741 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
    43801 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
    43861 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
    43921 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
    43981 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
    44041 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
    44101 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagg agggaaggtt
    44161 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
    44221 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
    44281 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
    44341 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
    44401 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
    44461 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
    44521 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
    44581 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
    44641 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
    44701 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
    44761 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
    44821 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
    44881 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
    44941 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
    45001 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
    45061 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
    45121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
    45181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
    45241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
    45301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
    45361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
    45421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
    45481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
    45541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
    45601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
    45661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
    45721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
    45781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
    45841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
    45901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
    45961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
    46021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
    46081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
    46141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
    46201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
    46261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
    46321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
    46381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
    46441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
    46501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
    46561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
    46621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
    46681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
    46741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
    46801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
    46861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
    46921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
    46981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
    47041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
    47101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
    47161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
    47221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
    47281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
    47341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
    47401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
    47461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
    47521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
    47581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
    47641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
    47701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
    47761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
    47821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
    47881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
    47941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
    48001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
    48061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
    48121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
    48181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
    48241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
    48301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
    48361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
    48421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
    48481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
    48541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
    48601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
    48661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
    48721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
    48781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
    48841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
    48901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
    48961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
    49021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
    49081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
    49141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
    49201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
    49261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
    49321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgttgga
    49381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
    49441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
    49501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
    49561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
    49621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
    49681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
    49741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
    49801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
    49861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
    49921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
    49981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
    50041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
    50101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
    50161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
    50221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
    50281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
    50341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
    50401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
    50461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
    50521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
    50581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
    50641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
    50701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
    50761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
    50821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
    50881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
    50941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
    51001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
    51061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
    51121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
    51181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
    51241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
    51301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
    51361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
    51421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
    51481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
    51541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
    51601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
    51661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
    51721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
    51781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
    51841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
    51901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
    51961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
    52021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
    52081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
    52141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
    52201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
    52261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
    52321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
    52381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
    52441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
    52501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
    52561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
    52621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
    52681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
    52741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
    52801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
    52861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
    52921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
    52981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
    53041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
    53101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
    53161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
    53221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
    53281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
    53341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
    53401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
    53461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
    53521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
    53581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
    53641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
    53701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
    53761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
    53821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
    53881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
    53941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
    54001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
    54061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
    54121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
    54181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
    54241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
    54301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
    54361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
    54421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
    54481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
    54541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
    54601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
    54661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
    54721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
    54781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
    54841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
    54901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
    54961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    55021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    55081 cttagttaag taatcgaact ctctacttgc tgaaatatca gcaggtagag agtttttttt
    55141 gcaactagct tctgtatatg atgtttacaa agaatggtac aagaaaagat catccttgaa
    55201 aaaggaaagc taacaataac tagcatcata taagagcttt taaggataga acagtagaat
    55261 aggaatctat gagcaagtcc tctcgtataa ataaaatagg agggaagtag cagcatattt
    55321 ttcataaagt ggctctcttt cttcattttt actttggttg ctcctatttg aaaattcgag
    55381 cacactcaaa tacaataaaa agaaatggca agggagagga ttagatgaaa aaagaagaaa
    55441 aaatcgcgat tttacaagag atcatccgaa ttaaatcagt gaatggaaac gaaggtgaag
    55501 tagcagccta tttgaataag cttttggcaa agcatgacat aacaggagag attgtttctt
    55561 acagagatgg tagagataat ttgattgctc gttaccaaaa gggccaatca ggaaaagtgt
    55621 taggcctatc tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct tggacctatg
    55681 caccctttgc agcagaaatc catgggaacc gtttgtacgg acgtggtgct acagatatga
    55741 agagtggctt ggcagcaatg gtgattgcca tgatcgaact gaaagagtca ggaaaaccat
    55801 ttaacggaac agtcaaatta ttagccactg tcggagaaga agtgggtgaa ctaggtgggg
    55861 aacagctcac gaaagcaggc tatgtagatg acttagatgc cttgatcatc ggagagccaa
    55921 ctaattacag tttgatgtat acgcacatgg gatcaatcaa ctatacagtc acctcgcacg
    55981 gaaaagaagc gcatagctct atgccagacc aaggttataa tgcaatcaat cacctaaatg
    56041 aattcattac gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc gagaatcctg
    56101 tcctaggaaa aacaatccat aatgttacat tgatcagtgg cgggaatcaa gtcaacagca
    56161 tcccaagcca tgcgcagctc caagggaaca ttcgatcgat tccagaatac ccaaatgata
    56221 aaatcattgc attgctgcaa tcaatcgtca atgaattgaa tcaagagaca gactatcatt
    56281 tggaattgat gatcgattac aacaaaatac ctgtcaaagc tgatccagat tctcctttga
    56341 ttcattgcat tcagcaacaa ttcagccagc cattaccttt agttggagcg gcagcaacaa
    56401 cggatgcagc cgaatttacg aaagctaacc attccttcga ctttgtcgtc tttgggcctg
    56461 gtgtcgttac tttgcctcat caagtagatg agtatgttga aatcgataat tatttggata
    56521 tgattgaaaa atatcaagga attattttat cctacttagc gtaagatttt tttaataaat
    56581 gaaaaaacag agccagatta gcattatttt gctaatccag ctctgtttat tcagcctatt
    56641 tttctttttg aatatatcaa gcgttgtggc aacatagtga tgactaatga aacaacgacg
    56701 atagacaaca aacgatcaag gtagtctgta cctgcctgta ccaagatcac acttgttgtc
    56761 tgacttaaac ctaacccatg taatagctga acgatcatac tggatcctga ggaggtgatc
    56821 ccgccaaata actgaacggt aatgatcgaa gaaacgatcg ttccaggtac cgacaaaatc
    56881 aaagcgagta ataacagttt caagctgttt ttatctttgc cgagatatct tggcaaaatc
    56941 aatccagcaa gcaagcctgt gagaatttgg attggcgaat aatacagaga aaaaatatct
    57001 gtagtcactc cacttaaaat accgctggac aatcctgtca gcattccagg tacaggccca
    57061 agcaatgcac cagcaaaaat cgttccgata gaatcgagat agattggcaa acgaagccat
    57121 aaagcaatcg ttcctccaat ataattgatc ccaatacaca aagccatcac tgtaagtacg
    57181 cgagttgcca ttttgttttt attcattttg ttagcccatc tttaaccttt ccaaatcttt
    57241 cataatcgtt tcttcttgtg catttaaaag aacggttaaa aactttcgga aaaaaagacg
    57301 agtatctact tttgtcataa ttttactgtt agcaggtttc tgccaaaatt cataacgatc
    57361 aaccaatgtt tgtccacgac ttattccttg agtttccaca gcagtgaagc ttttgaaacc
    57421 ttcacagatg gccccctcga taaaataagc aacagctaac ggatcattga tgacacatcc
    57481 taaaattctt tcttgtttcc agtggaagtc aaaataaaaa cgagtgatcg cttttagata
    57541 ctctccctct tcaggattca tttgacagca gtattcgagg atagtcggtg tcaagacgat
    57601 ttctcttgtc acatctaacc caaccatttc tatcgtttgt ttgagattct cgaatacata
    57661 agaagctgct tcaggatcac accagtaatt gtattcagca actggcgaac agttaccatg
    57721 gcttttgtaa gttccaccca tcgagacaaa acgatccata tgtttcccaa tatttgggtt
    57781 gactttcaaa gcaaaagcga tattggtcaa aggacccaaa gcaatgactg atgtatttgt
    57841 cttttctttg aaataatcag ccaaaaaatc aacggcatgg agaggttctg cctgttttct
    57901 caagatcatc gggaaatttg tttcccctaa accatccata ccatgagtgt cttgtgcact
    57961 gatgaaaggt tgcttcaaag gtttttccat tccttgataa acaggaatgt ctaaacgatc
    58021 acagcgttcc aggcatttca gcacattctg cgtacctata tctgcaggga cattcccaca
    58081 aactacggta atagcaacta cttcgatatc aggggatttg acagctaatg ataaagctaa
    58141 agtgtcgtcg atccctggat cgcaatcaat aattactttt cgcattattt ccaatctcct
    58201 ttctttagag atacttagtt ttttattctg ggaagtttcc gaaccagtcc aagacgagcg
    58261 tcttgattat aatgcacgaa attagtatac tggcttttat tgcgaatgta aatgctctct
    58321 tacaagtaaa tctattgagg agaactttaa gtaaatgata caatgaaaaa gaaatggaat
    58381 ttacgtgtgc cgaaaataaa gaatcctatg gatcggtaca ttggataata gttagattat
    58441 agtttggcag aaagcggcgg gtgtacaatg gttatttttt atttcattct tattctgata
    58501 attataggga tcagttacca ttttctttct tatttttatg caaagaaatt agtaagagcg
    58561 ggagtacaga gaggatatga ctggtatgaa gacactggtc atcgtttgat ggaccctgct
    58621 gcagtcacgt cagtagaaaa aaaacgacaa aaattagaag aagagtgtga aagcttttgg
    58681 gagcagggaa cacccaaaac aatcaaaagt tatgacgggt tgaaactagc tggtcagatg
    58741 ttcatccatc caaataagca gaataaatgg gtcatttgcg tacatgatta ccgaagcaca
    58801 gggaaaagag acatgtctca tataggtaaa agatatgcag agaaaggatt caacgttctt
    58861 gtacctgatt tacgtgcgca cggagaaagt gaaggagaaa tcatcggaat gggatggcta
    58921 gatcggctag atttaattgc ctggatccaa ctcatcttag atgaacagcc gaatgcgtcc
    58981 attattttac acggcggatc aatgggagct tctacgatca tgatggcaag tggtgaaaaa
    59041 cttccatcag cggtcaaagg atttatttta gacagcggat atgtttctgt ttatgcagaa
    59101 ttccgatata tgttaagtaa aatcactgtc tttcctaaaa aaatggtcat gagatatgcg
    59161 aatcattatg cccagaaata cgccggttac tccttaaagc aagcatcagc tacgagacag
    59221 cttggcagca accatctgcc tttattggtc attcatggtg aaagagatca ttttgtccct
    59281 acagaagcag cgtacacgat tcaaaatgct acggcagggg ataaagcact gctgcttgtt
    59341 ccagaagcag aacacttgga agcatcgatg aaagatccga aaacctattg gacagtgatt
    59401 ttttcattta ttgaacaaag aataacgatg aattaatttt ctcttctttt ttgtaagtaa
    59461 ttcgaggata aataattgtt tatagatcag gagactgcta aaataaggta ggaataaaaa
    59521 aatatcagtt atttgaggag tgcggcatat ggaaatcaaa gaagaaaaaa atcgtcttgc
    59581 tttattcaat gatgaacaac aagagatcgg agaaatgaca tggtcagatg cgggcccaga
    59641 catcatgatc attgaccata catttgtaga tccagcatat cgtggtcaaa agttagctga
    59701 aaagctagtg tatactggag tagaattagc aagacgagac gggaaaaaga tcattccttt
    59761 atgcccttat gcaaaaaaag agtttgaaaa gaaaccagaa tatcaagacg tcttacgtca
    59821 gtcataagaa taacagctga aagttttgaa ctttcagctg ttattccatt tatatagagt
    59881 tgtcacggac ttttatctat gataaagtta aagagacgat atcttttggg aaggaggctg
    59941 cgatatgtat aatttaattt tgggaatcgt gatcgtttta tcgattgtta tggtcattgc
    60001 gattatgatg cagccaagca agcaaaatag tgcagcgagt gcatttaccg gcggtgcgga
    60061 tcaactattc ggcaaacaaa aagctcgcgg ctttgaagct gtcatgcagc gatcaactgc
    60121 agttatggga gctatctgga tgatcttatt atttgtccta gcatatttat cttcgaagta
    60181 gttgaataag cctctcgcaa aacggcgggc ggcttttttt attgcaaaga gaacgcttaa
    60241 gaagaagtta tgaaagaaac tatggtaaaa tgaggcgaaa gaggtgagac gagtgagaca
    60301 attaccaaaa cccctttatg caaaacatgg aaagcgagcg gttttgctgc ttcacgctta
    60361 ctcaggaagt ccaaacgatg ttcgaatgct cgcacgattt ttggaaaaag cagattatac
    60421 agtttatgcg ccgcttttta aaggacacgg aacaatggag ccatatgata ttttgcaaga
    60481 aaaagcagaa agctggtggg cagacactaa aaaagccatc cattttttac agtctgagca
    60541 gttttctgat attgccgtat taggattatc aatgggcggc atctttgctg tacgagcact
    60601 agaagaagaa tcagtgattg gcggcggttt tttctgttcg cctttgtctc ctgtaaaaac
    60661 aaacgtgcct gaaaactttg aaaaatacgt acgtcaagtg ctgaagacag caggtaaatc
    60721 agagaaagaa atcaatgaaa aagcagttgc ttaccgctcg ttagctgaac aacaattaat
    60781 ggatatacaa gatcaggcag caatcgtaga aagtaggttg tcagatatcc aacagccgat
    60841 atttttagct caagctggga aagatgaaat gatcgaccct aatggcgtat ttgaaactgc
    60901 acgcaagttg agccgtcagc gagtgacact tcgatggtat cctgaaagtg gtcatgtgat
    60961 tacagttggt acagcaagac gcgaattaga aaaagacgtc ttggaatttt tagaaaaatt
    61021 accttggaat gaggaataaa tatgacaaaa gaaacgataa aagagaaaat tctctttttc
    61081 atggaaagca gtcgcaaacg cagtttttct atggaagaaa tcgctgaagg tttagggttc
    61141 caaaaaagcg atgattttaa attactagta caaacgattg ctcaaatgga aagagaacag
    61201 agtatagttt tcaataaaaa aggaaaagtg aaacttcctt taaaaccaat cttgatcgaa
    61261 ggaacatttc gagcaaatga acgaggattt ggatttgtaa cgattgatcc cgaagaagat
    61321 gacatctata tcccaaaaga agcgactggt tttgcaatgg atggagacac cgtagcaatc
    61381 gatatcatga agcgagcaaa tacagatatg gatcgcggag cagaggggaa agtagtcgag
    61441 attcgagacc gggcgactac tcagttagca ggggaattcg ttgcttattc agaagaagaa
    61501 atacaagaaa cggatcttta cggcgttgtt atcccgaaag atagaaagct caaccaattt
    61561 aaagtatatg cagcagcaga gggagttcgt ccagttgacg gaagtattgt gctgatcgaa
    61621 ctgacccatt accctgaaaa agggtatgcg actagtttag aagggataat caagcaagtg
    61681 attggtcata agaatgaccc tggcatggat atcctctcaa ttgtcgtggc taatggtatc
    61741 cctacgaaat ttcctgatga tgtattgtct gaagcagatg aagttccaga tcaaatcagc
    61801 gaaaaggata taactggacg tagagattta agaaatcagc tcattgttac gattgatgga
    61861 gaagatgcaa aagatctaga tgatgcagtt acggttaaaa aattagataa cggtaattat
    61921 tttcttggag tacacattgc agatgtttct tactatgtga cacaaggcag tcaattggac
    61981 agagaagcat atgaaagagg gacaagtgta tatttgactg atcgagtgat tccgatgatc
    62041 ccgcaacgtt tatcgaatgg tatctgttca ctgaatcccc atgttccaag gctgaccatg
    62101 agttgcgaaa tggagattga tccaaatgga caagtagtct ctcatgatat tttcccaagt
    62161 gtgatccaaa caacagaacg gatgacctac acagctgtca atcaaatctt agaagatcaa
    62221 gacgaagaaa cgatgcaacg ctatgctcag ctcgtaccta tgttccaaga aatgggtgaa
    62281 ttgcatcgaa tccttgaaac gatgcgaatc aaacgtggcg cgatttcttt tgaggatcgc
    62341 gaagcaaaaa tccttgttga tgctgaagga cacccaaatg atatccagct aagaagcaga
    62401 ggaattggtg aacgcttgat cgaatctttc atgcttgcgg ccaatgaaac ggtagctgaa
    62461 cattataata aattaaatct gccgtttatt taccgaatcc atgaacagcc aaaagaagaa
    62521 aaaatgcagc gcttctttga ttttgcttca gctttgggca ttctggtccg aggaacgaaa
    62581 ggaacgatca caccaaaaga tttacagcat gtgatcgaag gagtagaaga caaaccagaa
    62641 gctgcagtta tcaatactat gttgctaaga agcatgcaac aagcacgcta ttcagaagac
    62701 aattttgggc attatggctt agcggcagat tactacacac actttacatc tcctattcga
    62761 cgctatcccg atctaatcgt ccatcgtctg atccgttctt acagtgaaga taaaagtgaa
    62821 agcaatcaaa gttcgtgggc tacggaactt cctgaaattg cagatcatag ttctaagatg
    62881 gaacgtcgtg cagtagaaac agaacgtgaa gtagattcca tgaagaaagc agaattcatg
    62941 atggacaaag tgggggaaga attcgatggg atcatcagct ctgttgtgaa attcggcctg
    63001 tttatcgaac tgccgaatac agtagaggga ttgatccata tcaatgaatt gaaacaggac
    63061 tacttccatt ttgttgaaaa tcatttagct cttgtaggtg aaagaactgg tctgactttt
    63121 aaaatcggac agaaagtgcg agtaaaagta gtcaaagctg atccagatga acgagcagta
    63181 gattttgaac ttgtttccgc agaagaggtt acagcgttgg aacggccaaa aacacgaaat
    63241 ggaaaatcag gaaaacgcga aggaaaacaa gcaggaaaac gcccagaaag aaatggcaag
    63301 aagaacaaac gatatagcga tagtaagcaa acgttcggta agaaaaaagg gaagaagcca
    63361 ttttacaaag gtgttaaaaa gaagaaaaag aaaaaataaa atgagttgga ggaaacagct
    63421 atgccaaaag gcgagggaaa attaattgca caaaacaaga aagctcgcca tgattattcg
    63481 atcatcgaca cgatggaagc agggatggtc ttgcaaggaa ccgagatcaa gtcgatacga
    63541 aacagccgga tcaatctaaa agatggattt attcgcgtcc gcaacggaga agctttcttg
    63601 cataatgttc atatcagtcc ttatgaacaa ggaaatattt ttaatcatga tccgttgcgc
    63661 acgagaaagt tattattaca caaaaaacaa atcatccggc ttgaaaatga attgaaaaat
    63721 actggaatca ctgttgttcc tttaaaagtc tatattcgta acggctatgc caaggtattg
    63781 attggtctgg cgaaagggaa aaaatcttat gataaacggg aagatttgaa acgaaaagat
    63841 atcgatcgac aaattgatcg aacattaaaa aatttctcta gataatttca ttatcctttt
    63901 tcgcatattc ggtgcttttg cttttactta atgataaaat ttgttagcat ttaatctgat
    63961 aaaacatgat tatcataggt tttttcttag atcaggagca ccgtgtataa agaaaaattc
    64021 gcttcgtttt tgactgaaat aaaaaagtgt ctaattttca tgaaaatgtt ttgaattttc
    64081 tgtttcatgg tggtatgata ccgtgtggtt agaaaccgat tggaaaaaac cagatacgca
    64141 catgaattga ttttatgtga atttcgggga aaagaggtga aatggattgg atgaacgctc
    64201 gctaacgttc cacattggac caatttggtt tgatggtaca gtttgtataa tggtgttact
    64261 gacctgtttg attgttttct tcttagctta cttttttaca agaaatctta agttgaaacc
    64321 aacaggcaaa caaaatgcga tagaatgggt cattgatttt agtcgaggaa tcgtaacaga
    64381 taatctgcct agaaaagaat tgagcaattt ccatttgttg gcatttacat tgtttttatt
    64441 tgtttttgtt gcaaacaaca tcggtttagt aacaaaaatc gttttaccta acgaaacaac
    64501 actttggaaa agcccgacag cagacccgtt tgttacttta actttggcat ttataatgat
    64561 cttacttact cacttatttg gggtgaaaaa gttaggcttc aaaggatatt tcgtaaattc
    64621 gttcttgaaa ccatatagtt ttatgtttcc gatgaaactg attgaagaat ttacaaactt
    64681 attaacactt gctttacgtc tttatgggaa tatttatgcg ggggaagttt tgctaacctt
    64741 gattgcaaaa atggtaataa gcttaggctg gttctcattg ccgttagcaa ttccattaga
    64801 gatggtctgg atcgcattct ccttgtttat cggaagcatc caagcatttg tcttcgttac
    64861 tttatctatg gtttatatga gccataagat tgaagtggaa gaataatatt cgttttaaaa
    64921 acaaactact atttatctaa tttaggagga aaacaattat gaattatatc gcagcagcaa
    64981 tcgcaatcat gggagcagca atcggagccg gttatggtaa cggtcaagtt atctctaaaa
    65041 caatcgaatc aatggctcgc caacctgaaa tgtctggtca attccgtaca acaatgttta
    65101 tcggggtagc tctagtcgaa gctgttccta tcttaggggt agttgtcgcc ttgattctag
    65161 tttttggtgt ttaattgaat cagtattaag gcagaaacaa tttggtttgt tactgctttt
    65221 ttacatgaaa actagaagta cttcaaagaa aggaaggctc gcctatgctg aatcaattgg
    65281 caattgcaga agttggcaat gcgatgttag gaaatgtcat cgttgtcagc ggctcatttt
    65341 tgatactact ggctctcttg aagcattttg cttggggacc aatcagcgat attttgaaaa
    65401 aacgtgaaga caagatcgcc aatgatttgg attctgcaga acaatctcgc atcaactcag
    65461 cgaaaatgga acaagaacgc gaacaacaat tgttagcctc tcgttctgat gcagctgata
    65521 tcatcaaaaa tgcgaaagaa agtggagaat taagccgcca aaatattttg aaggatgctc
    65581 aagaagaagc agctcgtcta aaaagtaaag cccaagctga tatcactgta gaacgtgatt
    65641 cagcgctgaa ctctgtaaaa gacgatgttg cagaactatc tcttcaaatc gcggaaaaaa
    65701 tcttgaacaa agaattatct ccagaaatgc acgaatcatt aatcaatcaa tatatcgaag
    65761 gtctaggttc ttcaaatgaa actagataaa tatacagtag gaagacgtta cgggaaagct
    65821 ttatttgaac tggcgatcga ttcaaataaa gcagaagaaa tttatcaaga tctcgtaagc
    65881 ttacgtcaaa tctatgaaca agtacctgga ttaggtaata tgttaagtga tgttcgtcta
    65941 gaacctcatg aaaaacgaga aatcatggac aagcttgtta gtggctatga tggaatcgtt
    66001 aaaaactttt tagaagttgt ttatgcgtat aaccgaatgg atgatctttt gttcatgatc
    66061 gaagaatatg aacgtcgata tgatgaacat caaggtttgc tgttgggaag tgtcacaaca
    66121 gcagttccac tttcagacga acaacgaata caattagaaa agaatgttgc aaaaacaatg
    66181 aactatcaat cagtcgaatt aaaacaaatc gttgattctt ctattattgg tggcgcgatt
    66241 gttgaagcta accatcgagt aatcgacgga agcattcgca cgcaattaga aaatatgcgt
    66301 aatcaattaa atagatagta caaagaggta ggtgaatcga atggccatca aagcagaaga
    66361 aattagtgcc ttgattaaag aacaaattga aaattatcaa aacgtgcttt cagtcgaaga
    66421 aatcggtacc gtaacttatg taggtgacgg gatcgctcgt gcacatggtt tagaaaacgc
    66481 gatgagtggt gagttgcttg aattttcaaa tggctcatac ggaatggcgc aaaacttaga
    66541 atcaaatgat gtaggtatca ttatccttgg cgatttcgag tccattcgtg aaggagataa
    66601 agtaaaacga acaggaaaga tcatggaagt tccagttgga gaggccttga ttggacgggt
    66661 agttaatccg ctaggtcagc caattgacgg actaggtgaa atcgttactg acaaagctcg
    66721 tccagtagaa gcaatggcac caggcgttat gcaacgtaaa tctgttaacg aaccaatgca
    66781 aacaggtcta aaagcaatcg acgctcttgt accaatcgga cgcggccaac gtgaattagt
    66841 tatcggtgac cgtaaaacag ggaaaacttc cattgcgatc gatacgatca tcaaccaaaa
    66901 aggtcaagat atgatctgta tctatgtagc aatcggacaa aaagattcta cagttcgtac
    66961 acaagttgaa acattgaaaa aatatggcgc aatggattac acaatcgttg tgaatgccgg
    67021 tgcgtctcaa ccagcgccgt tgctttatat cgcaccatat gctggtactg caatgggtga
    67081 agagttcatg tacaacggga aacatgtctt gatcattttt gatgatcttt caaaacaagc
    67141 tgttgcttat cgtgaacttt ccttgctgct tcgccgtcca ccaggtcgtg aagcttatcc
    67201 aggggatgtt ttctacttac actcacgctt attggaacgt gcagcaaaat taagtgatga
    67261 tttaggtggc ggttcaatga ctgccttacc atttgttgaa acacaagctg gagatatctc
    67321 tgcttatatc ccaacaaacg ttatttcgat cactgatgga caaatcttct tagaaagcga
    67381 cttgttctac gcaggtactc gtccagcggt tgatgccgga ctatctgtat ctcgtgtagg
    67441 gggttctgcg caaatcaaag caatgaaaaa agttgctggg actttacgtc tagacttagc
    67501 aagttaccgt gaattagaag catttacaca attcggttct gacttggatg cagcaacaca
    67561 agcaaaacta aatcgtggac gccgtacagt agaaatcttg aaacaaaaat tgcatgcacc
    67621 gctagctgtt gaaaaacaag tagtgatttt gtatgctttg acacatggat tcttagatag
    67681 tatccctgta gacagtattt tagacttcga acatgaatta ttcgaatatc tggataccaa
    67741 tcattcagat atctttgaaa caatccgtac aacaaaagat cttccagaag aagaagcgtt
    67801 gaacagtgcg attcaggaat ataaagacat gttcttagct acaaaaggaa ataattcctc
    67861 tacagaagat aaattgaaat ccattcaaaa tgcatagtga ggtgagatga atgggtgctt
    67921 cattaaatga aatcaagaca cgtatcgctt caacgaaaaa aacgagccaa atcacgcggg
    67981 caatgcagat ggtgtctgct tctaagttga caaaatctga agcttcttca cagaagtttc
    68041 aggtttatgc aacaaaagta agagaaatcg ttacgcattt gactgctact cagttaaatg
    68101 atattgcctc agaaaatcct agaggggata tcaattacaa cagcatgttg ctcagccgtc
    68161 ctgttaagaa aacgggttat atcgtcatca ctgcagatgg cggtttagtc ggtggatata
    68221 acagctcgat attgaaacaa acgatgtcta ttttagaaga agaccaccaa tcacccgatg
    68281 attacgtgat gatcgcaatt ggcggaactg gtgcagactt ctttaaagca cgcggtatca
    68341 atctagcata tgagctgcgc aatctatctg atcagccaag ctttgatgaa gtccgcaaaa
    68401 tcgtaaatat ggcaacaact atgtaccaaa acgaagtatt cgatgaatta tacgtatgtt
    68461 ataaccatca tatcaattcg ctaactagtc agtttcgtgt agaaaaaatg ctgccaatct
    68521 cagacttaga tccagaagaa gcaactactt tcgaacaaga atatattttt gaaccttcaa
    68581 aagaagaaat cttgtctcag ttattgcctc agtatgctga aagtttgatc tacggtgcga
    68641 tcgttgacgc caaaacagca gaacatgctg ctggtatgac agcaatgaaa acagcaactg
    68701 ataatgctgc taatatcatt gatgagctga cggtttctta caaccgtgca agacaagggg
    68761 ctattaccca agaaattacc gaaattgttg ccggagcttc ggcactagaa tagtattgag
    68821 attggaggaa aaaagatgag ttcaggcaag attgttcaag taatcggtcc cgttgtcgac
    68881 gtggaatttt ctttagacca atccttacca gatatcaaca acgcattagt tgtttataaa
    68941 aatgacgaaa ataaatcaaa agttgttctt gaagcagcgt tagaattagg ggacggagtg
    69001 atccggacca ttgcgatgga atcaacagat ggtttacaaa gaggaatgga agtcattgat
    69061 acaggcaaag caatctctgt tcctgtaggt aaagaaacat taggtcgcgt attcaacgta
    69121 ctaggagata cgatcgattt agaaacacct ttcccagaag atgcggaaag aagcgaaatt
    69181 cataaaaaag caccagcctt tgacgaatta agtacaagta cagaaatttt ggaaacaggg
    69241 atcaaagtta tcgatttgct tgccccatat ttaaaaggtg ggaaagtcgg actattcggt
    69301 ggtgccggtg ttggtaaaac cgtactgatc caagaactga tccataatat cgcccaagaa
    69361 catggtggta tttctgtatt taccggtgta ggtgaacgta ctcgtgaagg taatgacttg
    69421 tattatgaaa tgaaagattc aggagttatc gaaaaaacag ccatggtgtt cggacaaatg
    69481 aacgaaccac caggtgcacg tatgcgtgtt gctttgactg gtttgacgat tgcggaatat
    69541 ttccgtgatg tagaaggtca agatgtactg ttgtttatcg acaacatttt ccgtttcact
    69601 caagctggat ctgaagtatc agccttgtta ggacggatgc cttctgcggt tggttatcaa
    69661 ccaacattgg caacagaaat gggtcaattg caagaacgta tcacatctac gaaaaaaggt
    69721 tcgatcacat cgatccaagc aatctatgtg ccagccgatg actacactga cccggcgccg
    69781 gcaacagcat tcgcccattt ggatgcgaca accaacttgg aacgtaaatt aacagaacaa
    69841 gggatttatc cagcggtaga tccattggct tcttcttcaa gtgctttagc acctgaaatc
    69901 gtgggagaag aacattataa agtagcaaca gaagtccaac acgtattgca acgatatcgt
    69961 gaattacaag atatcattgc tatcttaggt atggatgaat tatccgatca agaaaaagtg
    70021 ttagtttctc gtgcccgcag agtccaattc ttcttgtcac aaaactttaa cgtggctgaa
    70081 caatttactg gtcttccagg ttcatatgtc cctgtagaag aaacagtgaa aggattccgc
    70141 gaaatcttgg aaggcaaata cgatgatctt cctgaagaag ctttccgaag tgttggccga
    70201 atcgaagacg tcgtagaaaa agcgaaaaca ctaggctact agaaaggggt gccccatgga
    70261 tcaatattta acagtaaatg tggtaactcc tgccggtttg gtctatgatc accatgcagc
    70321 tatcgtcgtt gcacgtacga cagctggtga gcttgggatt ttgcctaaac atgcaccaat
    70381 catcgttcca ttgacgattg atgaagttcg tgtaaaacga acagattcag atacccacgt
    70441 cgattggatc gctgttaatg gcggaatcat ggaagttcgt gataatgttg tttcaatcgt
    70501 tgctgatagt gcggaacgtg aacgtgatat cgatgtatct cgtgcggaac gtgcaaaaca
    70561 acgcgcagaa cgccaaatcg aagaagcaaa agcaaaacaa gatacgaacg aattgaaacg
    70621 ggcaactgtt gcgttgcacc gagcaatcaa tcgtatcaaa gtatctaagc actcataaaa
    70681 tgagatgcgg atgtttatcc gtgtttaaga acagtaaaaa gaggttatga cagaagtggt
    70741 cagcttcaag acataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatctc
    70801 agcttatttt tcgaagaagc tacttctgga acaccgtata tcggaggttg tgaaatcagc
    70861 gttttgtcct gagtattaga gaaacaaatg gaaaatagtt cctcatattt tgccgttttt
    70921 tgagctaata ccgagggtca gatgattgaa caccgtttat ttggaataag ggactgcgac
    70981 aagac
//
LOCUS       DACEMK010000003        68781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEMK010000003 DACEMK010000000
VERSION     DACEMK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.003"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            233..475
                     /locus_tag="IW715_000136"
     CDS             233..475
                     /locus_tag="IW715_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1020770.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            472..942
                     /locus_tag="IW715_000137"
     CDS             472..942
                     /locus_tag="IW715_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1020771.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1070..2284
                     /locus_tag="IW715_000138"
     CDS             1070..2284
                     /locus_tag="IW715_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1020772.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2994..3182)
                     /locus_tag="IW715_000139"
     CDS             complement(2994..3182)
                     /locus_tag="IW715_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020773.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3273..3482
                     /locus_tag="IW715_000140"
     CDS             3273..3482
                     /locus_tag="IW715_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1020774.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3594..5207
                     /locus_tag="IW715_000141"
     CDS             3594..5207
                     /locus_tag="IW715_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1020775.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5242..6453
                     /locus_tag="IW715_000142"
                     /pseudo
     CDS             5242..6453
                     /locus_tag="IW715_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6476..7402
                     /gene="glsA"
                     /locus_tag="IW715_000143"
     CDS             6476..7402
                     /gene="glsA"
                     /locus_tag="IW715_000143"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1020776.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7610..9070
                     /locus_tag="IW715_000144"
     CDS             7610..9070
                     /locus_tag="IW715_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020777.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9291..11054
                     /locus_tag="IW715_000145"
     CDS             9291..11054
                     /locus_tag="IW715_000145"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1020778.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11138..11596
                     /locus_tag="IW715_000146"
     CDS             11138..11596
                     /locus_tag="IW715_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1020779.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11705..12388
                     /locus_tag="IW715_000147"
     CDS             11705..12388
                     /locus_tag="IW715_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1020780.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12535..13149
                     /locus_tag="IW715_000148"
     CDS             12535..13149
                     /locus_tag="IW715_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1020781.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13165..13371
                     /locus_tag="IW715_000149"
     CDS             13165..13371
                     /locus_tag="IW715_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020782.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13912..15042
                     /locus_tag="IW715_000150"
     CDS             13912..15042
                     /locus_tag="IW715_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1020783.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15134..15889
                     /locus_tag="IW715_000151"
     CDS             15134..15889
                     /locus_tag="IW715_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1020784.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15947..17986
                     /locus_tag="IW715_000152"
     CDS             15947..17986
                     /locus_tag="IW715_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1020785.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18095..19246)
                     /locus_tag="IW715_000153"
     CDS             complement(18095..19246)
                     /locus_tag="IW715_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1020786.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19400..19930)
                     /locus_tag="IW715_000154"
     CDS             complement(19400..19930)
                     /locus_tag="IW715_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020787.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20029..20379)
                     /locus_tag="IW715_000155"
     CDS             complement(20029..20379)
                     /locus_tag="IW715_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1020788.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20536..22065)
                     /gene="ahpF"
                     /locus_tag="IW715_000156"
     CDS             complement(20536..22065)
                     /gene="ahpF"
                     /locus_tag="IW715_000156"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1020789.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22075..22638)
                     /gene="ahpC"
                     /locus_tag="IW715_000157"
     CDS             complement(22075..22638)
                     /gene="ahpC"
                     /locus_tag="IW715_000157"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1020790.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(22782..23444)
                     /locus_tag="IW715_000158"
     CDS             complement(22782..23444)
                     /locus_tag="IW715_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1020791.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23628..25274
                     /locus_tag="IW715_000159"
     CDS             23628..25274
                     /locus_tag="IW715_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1020792.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25265..26347
                     /gene="uxuA"
                     /locus_tag="IW715_000160"
     CDS             25265..26347
                     /gene="uxuA"
                     /locus_tag="IW715_000160"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1020793.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(26455..28008)
                     /locus_tag="IW715_000161"
                     /pseudo
     CDS             complement(26455..28008)
                     /locus_tag="IW715_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            28156..29297
                     /locus_tag="IW715_000162"
                     /pseudo
     CDS             28156..29297
                     /locus_tag="IW715_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(29423..30502)
                     /locus_tag="IW715_000163"
     CDS             complement(29423..30502)
                     /locus_tag="IW715_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1020794.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(30605..31045)
                     /locus_tag="IW715_000164"
     CDS             complement(30605..31045)
                     /locus_tag="IW715_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1020795.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31102..31512)
                     /locus_tag="IW715_000165"
     CDS             complement(31102..31512)
                     /locus_tag="IW715_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1020796.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(31692..32126)
                     /locus_tag="IW715_000166"
     CDS             complement(31692..32126)
                     /locus_tag="IW715_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1020797.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32226..33074)
                     /locus_tag="IW715_000167"
     CDS             complement(32226..33074)
                     /locus_tag="IW715_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1020798.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            33463..35229
                     /locus_tag="IW715_000168"
     CDS             33463..35229
                     /locus_tag="IW715_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1020799.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35331..36590
                     /locus_tag="IW715_000169"
     CDS             35331..36590
                     /locus_tag="IW715_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1020800.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            36737..38038
                     /locus_tag="IW715_000170"
     CDS             36737..38038
                     /locus_tag="IW715_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1020801.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38041..38892
                     /locus_tag="IW715_000171"
     CDS             38041..38892
                     /locus_tag="IW715_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1020802.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38905..39720
                     /locus_tag="IW715_000172"
     CDS             38905..39720
                     /locus_tag="IW715_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1020803.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39871..41037
                     /locus_tag="IW715_000173"
     CDS             39871..41037
                     /locus_tag="IW715_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1020804.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41267..41914
                     /locus_tag="IW715_000174"
     CDS             41267..41914
                     /locus_tag="IW715_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1020805.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            41998..42942
                     /locus_tag="IW715_000175"
     CDS             41998..42942
                     /locus_tag="IW715_000175"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1020806.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKVNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43077..44528
                     /locus_tag="IW715_000176"
     CDS             43077..44528
                     /locus_tag="IW715_000176"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1020807.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            44549..45385
                     /locus_tag="IW715_000177"
     CDS             44549..45385
                     /locus_tag="IW715_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1020808.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            45385..46044
                     /locus_tag="IW715_000178"
     CDS             45385..46044
                     /locus_tag="IW715_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1020809.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46170..46859
                     /locus_tag="IW715_000179"
     CDS             46170..46859
                     /locus_tag="IW715_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020810.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            46865..47821
                     /locus_tag="IW715_000180"
     CDS             46865..47821
                     /locus_tag="IW715_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1020811.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            47865..48377
                     /locus_tag="IW715_000181"
     CDS             47865..48377
                     /locus_tag="IW715_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1020812.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(48633..49181)
                     /locus_tag="IW715_000182"
     CDS             complement(48633..49181)
                     /locus_tag="IW715_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1020813.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(49270..50451)
                     /locus_tag="IW715_000183"
     CDS             complement(49270..50451)
                     /locus_tag="IW715_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1020814.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(50687..52195)
                     /locus_tag="IW715_000184"
     CDS             complement(50687..52195)
                     /locus_tag="IW715_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1020815.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            52354..52545
                     /locus_tag="IW715_000185"
     CDS             52354..52545
                     /locus_tag="IW715_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020816.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            52748..53665
                     /locus_tag="IW715_000186"
     CDS             52748..53665
                     /locus_tag="IW715_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1020817.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            53658..54731
                     /locus_tag="IW715_000187"
     CDS             53658..54731
                     /locus_tag="IW715_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020818.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            54879..55397
                     /locus_tag="IW715_000188"
     CDS             54879..55397
                     /locus_tag="IW715_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020819.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            55378..55770
                     /locus_tag="IW715_000189"
     CDS             55378..55770
                     /locus_tag="IW715_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1020820.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            55846..56781
                     /locus_tag="IW715_000190"
     CDS             55846..56781
                     /locus_tag="IW715_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1020821.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            56914..57693
                     /locus_tag="IW715_000191"
     CDS             56914..57693
                     /locus_tag="IW715_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1020822.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            57686..58003
                     /locus_tag="IW715_000192"
     CDS             57686..58003
                     /locus_tag="IW715_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1020823.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            58247..59857
                     /locus_tag="IW715_000193"
     CDS             58247..59857
                     /locus_tag="IW715_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1020824.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            60014..60862
                     /locus_tag="IW715_000194"
     CDS             60014..60862
                     /locus_tag="IW715_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1020825.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            61093..61572
                     /locus_tag="IW715_000195"
     CDS             61093..61572
                     /locus_tag="IW715_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020826.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            61605..63833
                     /locus_tag="IW715_000196"
     CDS             61605..63833
                     /locus_tag="IW715_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1020827.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            63962..65605
                     /locus_tag="IW715_000197"
     CDS             63962..65605
                     /locus_tag="IW715_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1020828.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(65678..66223)
                     /locus_tag="IW715_000198"
     CDS             complement(65678..66223)
                     /locus_tag="IW715_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1020829.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(66246..66731)
                     /locus_tag="IW715_000199"
     CDS             complement(66246..66731)
                     /locus_tag="IW715_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1020830.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(66721..67533)
                     /locus_tag="IW715_000200"
     CDS             complement(66721..67533)
                     /locus_tag="IW715_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1020831.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(67672..>68781)
                     /locus_tag="IW715_000201"
     CDS             complement(67672..>68781)
                     /locus_tag="IW715_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1020832.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        23018 a        12149 c        13515 g        20099 t
ORIGIN      
        1 agaaattgat tgtcgttttc ttttgcagaa atttaagtgc aggcaattat ggtagttgtc
       61 gtaattgctg tcagctcagt gtttatcact gatggtgtga aagatttacg gttggagatg
      121 ttcaataaga ttattggtgc ttaattattt ttattgtagt gctgatatga gaatgttata
      181 attggggtat aaaacataca aataaatttg ctgaaaaatg aggtatcaaa atatggaaaa
      241 tgaattactg gaaaacttag gaagacttca cacaactgaa cttggcgtta ttagaattaa
      301 aaagaactta tctttgaatg tagaaaatgt aattgaatgg tgcaaagaaa aaattagcct
      361 ttctaatgcg gaaatcacaa gaaaaggaaa aaactggtat ataacgattg ataattgtat
      421 tataacggta aatgcgtaca gttataccgt tatcactgct cacaaggtaa aatgataaat
      481 ttttctataa aggaattaaa cgcatttgca gttattgggc aggaagtaga actgactaat
      541 tatcaaaaaa agaatattca gattagtaca cagttttgga gaaagtttaa cagtagtttg
      601 aaaaaagcgt atctttcaca atcaggaaat tgggtaaaat atgcttttat ggaaagaaga
      661 aatggaaaac tttattattt ctgttctatt ccccaaagaa ccattacccc agagggcttt
      721 ctgtataaag aaataccgtc ttataaatat ttggttgtgg agcatattgg tgctatggat
      781 aaaatatatg aaacttatgg caagatttat gaagaaatta ttcctagtac atcgtatatt
      841 cctataaaag atattatcct acactttgaa aaatacgatt atcgttttca ttggaatagt
      901 gataattcag ttattgaaat ttggatacct attcaagatt agaagcaatt ttatatccat
      961 acacacaaag cagttagtct taggattgac tgctttttct atacagattt ttaatgacag
     1021 gcaattatca attatggtag ttgccttttt tgattggagg aattgaagaa tgaatgataa
     1081 aaactttatt gaagaactaa gacaaaagcg tgaggaatac ggagtaacac aaacaaggct
     1141 tgctgttgcc tgtggtatca gccgtgaata ttacaaccgc attgaaaaag gaaaacagcc
     1201 attgaatgac gaattaagag aaatcataga aaaacagatt gagcgtttca atccgcaaga
     1261 accactattc ttattgattg attactttcg tgtccgcttt cctactacgg acgcattggc
     1321 gattatccgt gatgtattac aactgaaatc taattacatg ctttatgaag attatggaaa
     1381 atacggatat gaaagcaaat atgtacttgg cgacatcaat atcatgtgtt ccatgcagga
     1441 gcatttaggt gttttattgg aactgaaagg caaaggctgt cggcaaatgg aatgttatct
     1501 gctggcacag gaacgctcat ggtatgactt catgctggat tgtatgacgg ctggtggtgt
     1561 gatgaaacgg cttgatttgg ctatcaatga taaagcagga atattggata ttccaaagtt
     1621 aaaggaaaaa tacaaggctg gcgaatgtat gtcctacttc cgtatgcaga aagattacag
     1681 cggtacagaa aaatgcggta gcgatttacc aaagaataca ggagaaacct tatatctcgg
     1741 ttcaacaagt agcgaattat atatgtgtgc ttatcagaaa aattatgagc agtatgtcaa
     1801 gaatggcaca gaaattgaag atacggagat taagaaccgt tttgaaatac gcatgaagaa
     1861 cgaacgagcc tattatgcgg ttgtagattt actgacctat cgggacgctg aacgcaccgc
     1921 tttttctatc atcaatcatt atgtccgctt tgttgacaga gaggacgaca agccaaaaag
     1981 tcaatggata acaaatgatg attgggcatg gtttgtaggg gaaaacagag agccgatacg
     2041 cttaaccaca aagccagagc cttacacttt acaaaaagcg ttgcattggc tacaaagaca
     2101 agtcgcacca accataaaaa tggtacaggc attagacaga gaaaatcgta caacgatact
     2161 gaaagatatg attgagcagg cagaactaaa agacaagcat aaacatttat tacaattaga
     2221 gaaatcaacc atagaagaac gtatagatac cgctgttcca caagagaatg acggtatttt
     2281 ttaatgtaat tgaaaaattt cagatttagt cagcaaagag ctgttttcat tccctatgtg
     2341 aagtgaaaga agaaaaataa attttttatt aaatctgcaa caaaatggct acttgcgtac
     2401 agatatggtg cgagaaaagg aaatctaaac tttttttgaa aataggtcat taaatcgggg
     2461 cttgctgtcc ctatatgaag tgaaagaaga aaaaagtttt gaaaaaatga cggaaattcg
     2521 tgattagtgg acagtcttct gtgaaaagag gaaaaacatc atcattttgt attcaaacag
     2581 gcattagcag ttgagtgttg atgtgaaaag atgaaaatac tttgttctca acttaacaat
     2641 ttaaggtttc cgttccctat atggtgcaaa gaaaatattt cattctattg ttggcagtaa
     2701 cggtatggtt tgggtagtta gggtgtgaaa aggaaaatat tcttttttca tcatcaactt
     2761 agcaaaatga cattttctat ccctatatgg tgcatgaaaa gaagttaaaa tttttccatt
     2821 taaacttagc aaatggacgt ttgctgtaca gatagtagtg aaaaaggaga aaatcaaccg
     2881 ttgattgcca aaatgaaccg ttccatttgt actaataatg gaaaaagttt tggattttgg
     2941 ttacgtttct cctcttttcc aacgcgatat ataggaaaga cgattttttt ctttcaatcg
     3001 ttctttgaca actgaataaa gctattcaat acgtttgaca gacagctagc cgttgaaaca
     3061 aatacgccac gacctttccc ctgcaagagc gagcgaaaac ctatttgtgt tcttgtaaaa
     3121 gatttgtcct cttttatcgc catgacctgt actgtcagat aatgatactc ccgtacagcc
     3181 acagcttgag cattaagagc gtcgcacgca atgggtacgg ctacataaga actatgcaga
     3241 ggtggaactc ctgtgggcta tgacaaaagc cgttgaattg cttagaaaga ttttacagaa
     3301 agggggtatg tgttattgat aatgctgtaa aaacaattca aaagaaaaat ggcaagtgtg
     3361 gcattggcaa cagaggaaag acaaagattg tagtaaaaga gcatttttcc gaaaagggca
     3421 aaacaatgga agaacttcta actgatgtca tgctggaaaa agcaaagcag acaatcgcat
     3481 aaaatcggtg cagttgtaag aaatagattg aatgacaaaa agtacccgtg ttatacttta
     3541 cttgcaagca ggtattgtaa acacgggtta gttataaagg aggataactg acaatgaaac
     3601 aacagattta caatactgca ctttatctta ggttaagccg agatgatgaa ttacagggcg
     3661 aaagttccag cattaccaca cagagaagta tgttgcgttt atatgcaaaa gaacatcatt
     3721 tgaatgtcat tgatgaatat attgatgacg gctggtcggg aacaaatttt gacagaccga
     3781 gttttcaaag aatgattgag gatatagagg caggaaaaat caactgtgtt gtaacgaaag
     3841 acctttcccg tcttggcaga aattatatta tgacaggaca atatacagaa ttgtatttcc
     3901 ctagtcataa tgtccgctac atagcgattg atgacggtgt agacagcgaa aaaggcgaaa
     3961 gtgagattgc accatttaag aacatcatca atgaatgggt ggcaagagat acaagccgta
     4021 aagtcaaatc agcatttaag acaaagtttg cggagggggc tcattacggt gcttatgctc
     4081 cgttaggata taagaaacac cctgacatca aaggaaaact gttggttgat gatgaaacaa
     4141 aatggattat tgaaaaaatc ttttccctag cctatcaagg ttacggaagt gcaaaaatca
     4201 caaagcagtt aagagcagaa aaagttccga cagcgtcatg gctaaatttt acaagatacg
     4261 gtacttttgc acatatcttt gagggaaaac ccgaaagtaa gcgttatgag tggacgattg
     4321 ctcatgtcaa ggcgatattg aaaagtgagg tttatatcgg aaacagtgtt cacaatatgc
     4381 agtctacggt atcgttcaag agcaaaaaga aagtgcgtaa gcccgaaagt gaatggtttc
     4441 gagtagaaaa cactcacgag ccgattattg acaaggaagt gttctatcgt gtgcaggagc
     4501 agataaaatc aagacgcaga cagacaaagg aaaaggcaac gccgattttt gcagggcttg
     4561 tcaagtgtgc ggattgtggt tggtctatga gatttggaac gaataagaca aataaaacgc
     4621 cttacagtta ttatgcttgc agttactacg ggcagtttgg caaaggtaat tgttctatgc
     4681 actacattcg ttatgatgtg ctgtatcaag ccgtattgga acgcttgcag tattgggcta
     4741 aggcagtaca gcaggacgaa gaaaaggtat tgaacaagat acagaaagct ggcaatgcag
     4801 agagaatacg ggaaaagaag aaaaaggcaa gtacattgaa gaaagccgag aacagacaaa
     4861 atgagattga ccgtttattt gcgaaaatgt atgaagatag agcctgtgag aagataacag
     4921 agcgaaattt tgtgatgttg tccagcaagt accaaaaaga acagatagaa ctggaacagc
     4981 agataacaag cctaagagaa gaactaagta aaatggaaca ggatatgata ggtgctgaaa
     5041 agtggattga gttaatcaag gaatattccg taccaaagga actgacagca ccattattaa
     5101 atgccatgat agaaaaaatc ctcatacatg aagcaacaac gaatgaggat aacgaaagaa
     5161 tacaggaaat tgagatatat taccgattta ttggaagagt tgagtaatca acaagagtaa
     5221 tatttttaac taagggaaac cggcggtgtc tatacttggg tcagtcgcac attgggaaaa
     5281 cgattcggtt ttgccgcaat ctttttccaa tggtttgaga taacagttgg ctacttgacc
     5341 atgctgtatt tcttaactgg tgcattatcc tatgcaacgg gtattggtgc tatacaaaac
     5401 aataaatatc tgaaattggc aatcctattg attattttct gggctatttt actctcacag
     5461 ttaaaaggaa ccaaatacac ttcattgatt gctcggatcg gttttgtagc tggtatcttg
     5521 ttgccagcat tagtcttgtt tggcttaggg atccattatg tcgcttcagg cgctccacta
     5581 caaacgatac tgtctatgaa aacattgatt cctgattttt cgaaattgcc tacgctggtt
     5641 gtttttgttt cctttatttt agcatatatg ggcgtagaaa catctgccag tcatgcaaat
     5701 gaaatggaaa accctaaaaa ggattatcca ttagcaatgt ttgtattggt tattctggca
     5761 atcattttag atacttttgg cggattgacc gtcgctacta cagtacctca acatggtttg
     5821 tctcttaata caggggtgat tcaatcgtta gaattcctat taagacacat tgatccaaat
     5881 cttgcatgga ttgcgaaaat tcttgcgatt cttgtttgtt taggtgtcat cggagaaatt
     5941 gcttcttggg taacctctcc ttcaaaagca cttcacgtag cagcagaaga aggattactt
     6001 cctgaatatt ttgcaaaaga aaacacacac ggtgttcctg ctcatttgat gatcgcaaat
     6061 gggatcgttg caacaatctg ggcagccgtc tttacattaa gcggcggggg gaataatctt
     6121 tcatttcttg cagcgatgtc attgactgtt gtaatctacc tgatgttgta ctttatgttt
     6181 ttcgcgggct atttgaagct ggtttttaaa gatgacagtc ttgaaagagc ctatcaggtt
     6241 ccaggcggaa aagttgtaaa agtaatcgtt gcttgtttag gtcttttgac ttcacttttt
     6301 gctttttgta tctcatttgt tccacctgct agtatcgcag ctggacagca tgggatttat
     6361 gaaacgattc tgattgtttc ttatcttatc gtcttagtta ttccatttac gatctacgca
     6421 tgccgtcata aattccagca tgcagaaaaa taaaaaatga aagctggtta ttcaaatgga
     6481 agtaaaagaa ttagaacaat tgattggaaa aaatttccac tattatcaaa caggacaagt
     6541 ggcggattac attccagcat tagctgaagt ggatcccaaa caattaggta tggcgattta
     6601 tgatttggat acaaaacaac tgattgaggg aggagacgcc gatgtgcgct ttgccatcga
     6661 aagcatgtca aaagttcccg tattgttatt agcaatcaaa gacaacggaa tagaaaaagt
     6721 ctttcaaaca atcaacacgg aaccaaccgg ttttgccttc aattctatca tgaatatgga
     6781 aatcaatcat cgcaaacatc caatgaatcc ttttgttaat gcaggagcga ttgcaacaac
     6841 ctcgttgatt gccggtaaag atgcagatga aaaatttgag cgaatcttgt catttatgaa
     6901 agagatctgt gatgatccta atatcacatt gaatgaagaa atctatcatt ctgaatcaag
     6961 aacaggtgat atcaatcgct ctcttgctta ctatatgaaa ggtaatcaaa tgatcgaagg
     7021 agacgtaccg gagatactcg atgcttattt cagacaatgc tccctcaatg tcaccgctac
     7081 aggcatagca aaattagctg ctgtgctggc aaataaagga attgctcctt ggaatggcaa
     7141 acaaatcatt actgaagaaa gtgctacaat cgtcaaatca attatgacaa ctgctggttt
     7201 gtatgatgaa tcaggagagt tttccgtcca tgttggtgta cctgcaaaaa gcggtgtagg
     7261 cggcgggttg atggcagctg tgccaaatcg ttacggcata ggtgtcttca gcccggcact
     7321 agatccattt ggtaacagcg cagctggtat ccaattgttg aaagatgtag tcaaagaact
     7381 agacgctgac atctttgaat aaccattctt gaaagaggct ggggtaaaag tccaaacggc
     7441 tcaaagctac ctacaatgag gctgaaattg tgagcttgtt tgacttttgt cccagtttct
     7501 ttttttattg gtttttgaaa gggcttttct aaataaaaag gaaacaaaaa tacgatttac
     7561 agatatgatt aaataaaatc ataagaaaaa ctggaggcgt gtatcaggaa tgctggaaaa
     7621 ttacatcgag cgaaatatat ttagaaaagt gtatctgtgt gaacaattat ttgaattcca
     7681 agaaatagac atcgaacaaa cagctatttc tctaagggtg actacaccaa cgatccttca
     7741 cgatctagaa agtctagccg agtgtctaga atattgtata aaagaacaag taagagagaa
     7801 gcataagtat aaactcgtat tcaaacatgg tattgctctt tctgagctca cacagtttct
     7861 gtacggtcaa tcttattttt taaagttttt aagttattat tttaatggta tattcacgag
     7921 tacagaactg gctgatcttg aatttatttc tttgagtaaa gtatatacaa tcaaaaaaat
     7981 cgtattggat ttttttaaag aaaatgatta tttgaaaaat aaagaaatca ttatcccaga
     8041 atttgactcg cggaacatct tgctagcttt agtacgttat gtcaattggg aaggctatga
     8101 aaataaaaat ccgtatgtag aagaagcttg taacgagttg attgattatg tagagactca
     8161 tttttttaaa agaagatatt cggaagatga aaagtccttt atcatacgag aaatagagat
     8221 cgcaatcgga agaaaggcat ttccccttgg actgaaagaa gaagataaga aaagcgcaga
     8281 aacgaagccg ttatttcaat tagtctgtca aggattggaa gagcaaaaag gaattctaga
     8341 cttacaagaa gaagatgtct attatatttt ttctttgttc aacagtagaa attataccaa
     8401 tgagaacatg gagctgttaa gaaaagattt cgaagttgtt tataaaaatt ttgttctcaa
     8461 tgacctatca ctgaatgaat tgattttaca gattattagt atcgctgaaa tcgacgatgc
     8521 agaaaatttt attttcaagc aatccttttt acaatttgtc cgtacgttgt gggcagatgg
     8581 acaagtattt ttaccggaaa ggatctatct tctatcagaa aaagaacaac ttctgtacgg
     8641 aaaaattgta gaaatattgg aagagtggaa aaagaaaaat caaatccaac taagatggaa
     8701 tgaaaatttc attcgaaaat tcgtcaaaag tctttctttg atcaacagtg aacaagcggg
     8761 gagacaggaa atagaaattt ttgttgtttc agaaagttca gtcaaacaat ttttttatcg
     8821 aacccaattt catttgtatg cggaagaagg catcgctgaa ataaatccta ttttatttcg
     8881 ttcattagaa gaattgcctg atgaatgtct atgtgcgaaa aaaagagtgg ttttgtgcga
     8941 tgctgctagc tatcaaaatg gattagaaac tgaaaagaca aagatctatc caatctcatt
     9001 aaaaggtgca aacatccatt tgtatcatat tgctcaggaa cttcacctat taaaaaaaca
     9061 tatttcttga tgagacctta attaggagac cttctacaaa gctttttaat tgtctgtaga
     9121 aggtttttat ttgagtcctg attcgtgcga agatctactt atataacaag ctttctctga
     9181 aattttttta aaatgctatt gacttagagt caactctaag tagtagaata gataaagtgt
     9241 aacaaatcta ttttcattaa aagaagatag tacggaggag agaatgtatt atgagtacag
     9301 tattacatgt attggatgca atcaatagcc ccgcagatgt caaaaaatta tctgttgagg
     9361 aaatgaaaca gttggcagaa gagatgcgcc aaaaaatctt gcaaaaggac agcgatgtag
     9421 gtgggcacgt tggtccgaat ctaggtgtag tagaattaac gattgcgttt cattatgtat
     9481 ttgattcacc aaaggataaa atcgtttggg atgtttccca tcagtcttat ggtcataagc
     9541 ttttaactgg tcgaaaaaat ggttttgaaa aaggacatta ccgtgaagtg agcggatatt
     9601 ccaaccaaca tgaaagtaaa catgactatt ttacagtggg gcacacatct acatctatca
     9661 gcttagcaac aggtttagct aaagcgagag atctgaagaa agaatcagga aatgtggtag
     9721 catttatcgg tgacggctcc ctaagtggtg gcttagcttt tgaaggattg aacaatggtg
     9781 gtgttttaca atccaatcta atcgtattag taaacgataa tgagatgtct attgatgaga
     9841 atcatggcag tttatataaa aacttagctg agcttcgtca aaccaacggt aaaagcagca
     9901 ataacctatt taaagcaatg ggatttgatt atcgttacgt ggataatgga aacgatctgg
     9961 aaacggtgat ccgtttattt gaagaagtaa aagatattga tcatccgatt gtacttcacg
    10021 tccatacgga aaaaggtcgc ggttatcagc cggcaatcga taataaaatg aaatatcatt
    10081 ggcatgtacc atttgatctg tccaccgggg aggcaaagca agtaacttct ggagaaacat
    10141 ttaatatgat catcatggat tatttggata agaaaatgca aacaagtaca gctcctattg
    10201 ccgcagtcaa tgcagcgata ccaggcgcct ttggactgaa aacattccaa gaaaaacatc
    10261 cagatcatta ttttgacgtg gggatcgctg aacagcactc tatcactttc gcttctgctt
    10321 tggcttctca gggagtacgt ccagtcgtct tccaatcaag tactttcttg caacgggcat
    10381 atgatcaatt atcacatgat ttagcgatca acgagaatcc agtgattatt ttagtcggtg
    10441 gcggaacgat ctcagctagt gaccgaacac accaaggaac atttgacatt ccttacttat
    10501 caagtatccc gaacatcaaa tatcttgctc cagcaacaaa agaggatatg atctctatgc
    10561 tagattgggc gttcgaacaa acagcgcagc ctgtagcaat ccgaattcct acacatggtg
    10621 tagaacatgg tacagctatc gcaaatgatt atagtattcc agcctatcaa acagtaaata
    10681 atggaggaaa agtagcaatt ttagctttag gcggttttct tgaattagga aaagaaacag
    10741 taaaagcttt tagaaaaaaa ggaatcgaag caacgttgat caatccagga acgatcacag
    10801 aaaatgacgt agaaacatta gataaattaa aagaaacaca cgaattagtc atcacattag
    10861 aagatggtag tttgtcagga ggatttggcg aaaatatcag tcgttattat ggtgattctt
    10921 caatgaacgt attaaatttt ggtgcagaga aagaattcac ggatagcgtt cctgtagggc
    10981 agctttatga acgttatcat ttaacaccaa aacaaatcgt tgaagatagt ctgcgtgtct
    11041 tgaatattca ataattcatt atgctcacca tccagataat tgtttatact agtatctgga
    11101 tggttttttc attataaaaa gcaaaggggt ggatgttatg tatcagcaag tagaagaata
    11161 tgtcaaaaag ttccaagaaa aatatcctag ttatcaatca gaaaaattag gtttcttttc
    11221 atttggggat acagaaaaaa tggcagacga gttgagcgaa ctagttgtaa aaaagataaa
    11281 gactggtaca tcaagtggtt acgatctgta taaacaagag aatgagcctt taccaaaagt
    11341 tggtcaatta gacgtggtcc tgaatggaaa aaatgaacca gtttgtatca tcaaaaatat
    11401 aaatgtgacc atcttgcctt ttagtgaagt cacgaaagag catgcttgga aagaaggaga
    11461 aggggatcgg acattagctt attggcggaa agtccatgct gagtttttta aacatgctta
    11521 taaagaagcg ttgaacatag aatttacaga agagaagctg gtcgtatacg aagaatttga
    11581 agtaattttc ttttagtaag aaaggtctgc tgaaagaaat agctcattat tgtctcctga
    11641 atagaaaagg gttacatttt tatatgaagc tgtattttta tataacatta ctggaggaga
    11701 tattgtggat acactggaac aaatcaaagg cggagtcgtc gtttcttgtc aagctttgga
    11761 aaacgagccg ctacacagtt cttttattat ggggagaatg gctttggctg caaaagaagg
    11821 cggagcagtt ggtattcgag caaatacgac agaagatatc aatgaaatca agcgaatcgt
    11881 tgatttaccg gtaataggga ttatcaaaag agattacgat gggtcagaag tgtttattac
    11941 tgcgacagaa aaagaagtgg aagaactttt ggagacgcaa gcagaaatca ttgcactgga
    12001 tgcaacttct cgtaaacgtc cgacagaaaa aacaactgct gaacttgtcc aaatgattca
    12061 cgaacacggt cggttagcta tggcagatat ttcgactttt gaggaagcgc ttcaagcgca
    12121 agaagatggt tttgatcttt tgtctacgac tttagcaggc tatacagaat attcacgaaa
    12181 gacgcagaca cctgattttg atttattgaa tcaaatcata gaacaagtcg atatcccagt
    12241 aattatggaa ggacatactg actctcctga acaagtagag aaagcatatg aattaggtgc
    12301 tttttcagtc gtagttggat ctattatcac ccgtccgcaa ttgatcacta aaagatatgt
    12361 cgaagccgca agtaaagcaa ataactaaaa aacacgcacg gcacagattc ttgacaaaaa
    12421 agaaaaattg atagatagtt taaagcagat tctaagaaga tgaaaaaagc attacttttc
    12481 attttgtctt caagatctgc tttttaacga attttataga aagaaggcga aaccatgttt
    12541 gaacggagtc cttcagaatt gacagaacgt gaaaattaca agttgctgat tggttcagtc
    12601 atccctagac cagtagcagt agtaacgact aaatcagcaa aagatatagt gaatatcgca
    12661 cccttcagtt acttcaatat cgtcagttcg aatcgaccca tcttatcctt ggcgatccag
    12721 agaaaacaag atgcagtaaa agacacagca aagaatattc ttgagacaag agaagctgtt
    12781 atccatatcc ttgatgagga caacgtagaa gaagtaaata aaacagcagc caatctgcct
    12841 aatgaggaaa gcgaactgta cctgactgac ttgactttaa ctgattcagt aatcatttca
    12901 gtaccagcat tgacagaagc aaaagttcgt tttgaaacat ccttatatga acatgtcgag
    12961 atacggaata aaaataaagt aacagctgat ttatttttgc tagaagtaca gaaatatcat
    13021 ttaaatgaaa cagtctatga tgataagaca ggaaggatcg atccaagaaa attgaatgct
    13081 gtaagccgtt tggcaggaaa tgattatgca gcaataggag aaattttcac gattgcacgt
    13141 cccgaataaa aaggagcact agttatgtta aaaataagct ataaaccatc gacagattca
    13201 aaagaaatga aaaaagaata tgaaacagtg aatgattttc tgcaagggca gtacttagaa
    13261 gttccgccat tgcaagacca ttttgtcgtt actactgtta ctttagatgg taaggaaata
    13321 gaaatgccag atcaaacgat tagcggattg tttaattact ttaacaaata aatatttatt
    13381 ccgaaaagtg aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct
    13441 ggtgcaactg aaagaatttg caacgggatt ctttcaactt atttgttatt tttttctaaa
    13501 aaatattaaa gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag
    13561 gaaaagtcta tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc
    13621 tatacaaaca taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac
    13681 tgttggatta aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa
    13741 aactcggacg atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt
    13801 tctttatatt tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt
    13861 ggattgctgt gtgaggagga gatgcggtga aaaaagatcg attggtggat tttgggtcct
    13921 tatttgactt tgtccatgat cggtctttta gaagtataca gtgccagttc ttatcgttta
    13981 ttgcaagccg atgaaaatac aaaaagtcta ttattgcgtc aactaatttt catatttttg
    14041 agttggagcg tgatcttctt agctcgttca gtcaaactac actatttact tcaccctaaa
    14101 atagcaggat acggtttagc cttatcgatt ttctttttag tattagtaag aatagggata
    14161 ttcggtgtca ctgtcaacgg cgcacaacgt tggatctctc tgtttggcat tcaattccag
    14221 ccttctgaac tggcaaatct ttttttgatt ttttatttaa gctggttttt tcgtgacgga
    14281 aatagtagcc caaaagatct aaaaaaacca ttcctgatta cagtaggtat aacttttctg
    14341 attttatttc agccaaagat tgctggagca ttgatgatcc tttcgattgc gtgggtcata
    14401 ttttgggcag cggcggttcc atttaaaaaa gggatctatc taatcgttac tttttctgca
    14461 ttgctgattg gagcagcagg cggggtatta tatttaggaa ataaaggttg gcttccacag
    14521 atgtttaatc atgcttatga aaggatcgcg actttaagag attcttttat cgatagtcat
    14581 ggtgcgggct atcaaatgac ccattcgttt tatgcactat ataatggtgg catatttggg
    14641 cggggattag gaaatagtat cacaaaaaaa gggtatcttc cagagacaga aacagatttt
    14701 attttttcga ttattacaga agagcttggg ctaatcggtg cattgtgtgt gcttttctta
    14761 ttgttctcat tatgcatgag aatcttttgt ttaagcagtc gctgtaaaaa tcagcaagct
    14821 ggattgtttc ttttaggatt cggaactttg ctattcgttc agacgataat gaacgtggga
    14881 agtatcgcgg ggctgatgcc aatgactggt gtgccattgc catttgttag ctacggagga
    14941 acaagttatc tgatcctttc tttaggaatc ggaatcacgt tgaatatttc ttcaaagata
    15001 caggccgaag agttggcgct ctatcgacct gaaaaacaat aaagagaaac tgagaaactc
    15061 tcagtttgaa ttatcataaa gtacaatctt ttatttttca agcgagaggt tcttttagaa
    15121 agaggaattc gcaatgaaag tatctcaaaa aatcatatta tctattttgt tactgcttac
    15181 attagtagtt ggctattttt cgattgagtt tatccatgga ttttcgtctg caaaacagag
    15241 ttcaactgtg aaaaaggtag agccgaaaag tatccctacc acactaaatg tggctttgat
    15301 tggttcagat gctcgttcga aagacgaaaa cggtcgctcg gattcacttc tggttgcaca
    15361 atacaaccag aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga
    15421 tattccaggt tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt
    15481 attaaatcaa acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac
    15541 atttcaagat tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc
    15601 agaaaaatct ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg
    15661 taatacttta ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat
    15721 cagaaggcag cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat
    15781 attcaagcta cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga
    15841 aagtgtgctt ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt
    15901 ataaatagtg aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga
    15961 caagcacggc aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc
    16021 tgcgggtggc ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg
    16081 agaaaagaca gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc
    16141 agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga
    16201 taaataccag aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa
    16261 agtagataaa aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc
    16321 attaggtgaa ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac
    16381 cacgatcaac tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag
    16441 cctgaccaca caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc
    16501 aacaacaggc aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga
    16561 aaaaacagcc aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa
    16621 tcaggcgatt tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga
    16681 tggagcaacg ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta
    16741 tcctttgggt gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga
    16801 tattgataag aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat
    16861 ggcttttgat aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga
    16921 cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt
    16981 gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt ctaggaggaa aagctggatc
    17041 aactgttgcg acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta
    17101 tgatccaaac aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa
    17161 tcctgaacat ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa
    17221 aatgattaca gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac
    17281 gatcaacggg cttaaatggc aaaaagacag ttcttgggga tcttatcaag caacgcgtgt
    17341 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
    17401 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
    17461 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
    17521 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
    17581 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
    17641 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
    17701 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
    17761 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
    17821 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
    17881 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
    17941 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
    18001 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
    18061 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
    18121 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
    18181 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
    18241 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
    18301 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
    18361 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
    18421 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
    18481 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
    18541 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
    18601 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
    18661 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
    18721 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
    18781 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
    18841 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
    18901 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
    18961 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
    19021 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
    19081 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
    19141 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
    19201 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
    19261 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
    19321 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
    19381 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
    19441 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
    19501 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
    19561 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
    19621 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
    19681 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
    19741 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
    19801 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
    19861 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
    19921 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
    19981 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
    20041 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
    20101 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
    20161 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
    20221 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
    20281 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
    20341 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
    20401 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
    20461 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
    20521 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
    20581 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
    20641 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
    20701 catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac
    20761 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
    20821 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
    20881 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
    20941 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
    21001 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
    21061 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
    21121 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
    21181 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
    21241 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
    21301 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
    21361 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
    21421 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
    21481 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
    21541 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
    21601 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
    21661 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
    21721 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
    21781 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
    21841 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
    21901 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
    21961 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
    22021 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
    22081 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
    22141 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
    22201 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
    22261 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
    22321 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
    22381 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
    22441 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
    22501 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
    22561 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
    22621 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
    22681 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
    22741 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
    22801 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
    22861 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
    22921 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
    22981 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
    23041 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
    23101 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
    23161 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
    23221 gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt atacacgagt
    23281 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
    23341 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
    23401 ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat tcctccaaaa
    23461 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
    23521 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
    23581 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
    23641 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
    23701 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
    23761 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
    23821 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
    23881 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
    23941 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
    24001 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
    24061 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
    24121 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg ctactttata
    24181 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
    24241 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
    24301 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
    24361 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
    24421 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
    24481 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
    24541 aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
    24601 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
    24661 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
    24721 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
    24781 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct aatatgcctc
    24841 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
    24901 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
    24961 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
    25021 cagacccact attgacagac cttcagcata agctttccga tattcaactg ggtacgcaag
    25081 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
    25141 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
    25201 caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
    25261 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
    25321 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
    25381 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
    25441 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
    25501 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
    25561 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
    25621 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
    25681 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc cgggatggga
    25741 acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
    25801 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
    25861 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattagctcg
    25921 aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca gcccttataa
    25981 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
    26041 tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
    26101 tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt cattagacat
    26161 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
    26221 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
    26281 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
    26341 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
    26401 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
    26461 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
    26521 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
    26581 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
    26641 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
    26701 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
    26761 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
    26821 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
    26881 ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt gataaagcat
    26941 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
    27001 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
    27061 agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
    27121 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
    27181 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
    27241 atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
    27301 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    27361 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    27421 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    27481 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    27541 gccattatta gaaaaggaga tagacaaacc gtactatgct aaaaatgcat taatcttttt
    27601 cattttcgta tacaaataag aggcactata gtttgtttgc tgactgaaac tgataatcga
    27661 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    27721 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    27781 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    27841 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    27901 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    27961 gctttttttc ttcaagataa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    28021 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    28081 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    28141 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    28201 ttatcgctta ctataaatca gacagtccac acagaggaga cagaaacagc caaatggaca
    28261 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    28321 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    28381 tcgggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccact
    28441 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    28501 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    28561 gatggactac cgaacgatca tcatttaacg ccgtatagtc agcggcagac aattgagcaa
    28621 gattcagcca tcgactcagg aggagatctt gttgaagaac caacagataa accagaaaat
    28681 gaaaataagc cagaagtacc gcctacagaa aatccagacg gagaacagaa accggaaata
    28741 gaacctggag aagaacccga tacagaaact caaccagagc cagacaataa atcaaaacct
    28801 gaaataaccc ctggagaaaa accagatgta actccagaac cagatacgga ttctggaaac
    28861 caaacagtac cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca
    28921 gaggtagatc cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac
    28981 caaggaatac cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt
    29041 aaaaaatcaa atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga
    29101 atatttgata gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct
    29161 gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac
    29221 tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac
    29281 aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa
    29341 atatccagaa aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac
    29401 tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga
    29461 tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc
    29521 cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca
    29581 gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa
    29641 tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga
    29701 aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa
    29761 tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga
    29821 gcaacgtatt taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga
    29881 caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa
    29941 acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga
    30001 atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg
    30061 aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt
    30121 gtaaaacagg atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca
    30181 tgtcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt
    30241 aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct
    30301 gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg
    30361 taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc
    30421 gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac
    30481 aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga
    30541 aaaaccgcta aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc
    30601 agtttcagcc ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt
    30661 gcagtgtatc caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac
    30721 cttctgcttg tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt
    30781 catcacccgt ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg
    30841 gagtcagcgt accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt
    30901 tcttttccca taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga
    30961 ggaggggagt gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt
    31021 gattaactaa atatagatct tccatttttc cacctccgct gttttactat agtgtatcat
    31081 agcggattta cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc
    31141 agctgtagct tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc
    31201 ttccagagaa agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc
    31261 actttctgaa taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg
    31321 ctgtttgatc aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg
    31381 ttcagggttg gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt
    31441 acgatcggga ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata
    31501 catttttttc ataaaattac cacctttaat tatattttat acaattgaat tttacacaat
    31561 ataattgtgg aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa
    31621 cagacgcgct ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct
    31681 catcataaac attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca
    31741 cgatggacct gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc
    31801 acaccaaagt ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct
    31861 ttattatgtg tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt
    31921 tcagcagtag gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta
    31981 ccatacaagg taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt
    32041 tgtatttcat caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca
    32101 aaatcgattt ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac
    32161 tagattcttt tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta
    32221 ccaactcatt cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg
    32281 acaatgacaa aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca
    32341 tgatcagtca caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc
    32401 tctagcaggg taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa
    32461 taatgttcac tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc
    32521 atgacaattg aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa
    32581 ataaacagat taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc
    32641 aagtggaaat attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg
    32701 tttttatata aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac
    32761 aaccgatctg aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag
    32821 cttccgaaaa gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac
    32881 ataacgatat tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt
    32941 aatattccta gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg
    33001 atagcgactc ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt
    33061 ctcgtttctt tcatttattc acttccttaa ttcattaata atactcttat cttattataa
    33121 cttacttttc accttaagtt aactttaatt aataaaaaaa ttaggctagt ctaaattatt
    33181 atgtttcatt cttctagaat atacttctac gccaaaaact gttaaaaaac ttttacttta
    33241 gaaaattgga gaaattgatc gcttaagata aattcaggac actaaatagt acaggaagta
    33301 gactaaaact tttaaaagtt ttaagtctat ctatttggca agtttaaaaa ataaaaattc
    33361 ggtatcaaaa aactgttacc gataacattt ttgaaagcgt tgtcttttgc ttctctatca
    33421 cttatagtga tgacgtcata ttaaaacaca aggaggaaat caatggatac agctgcaatt
    33481 tatcatcgac cggaaagtga atatgcattt ctatatacga atacaagatt tcgtattcgg
    33541 cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc
    33601 ataacaactg aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat
    33661 gttcatgatt attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc
    33721 catgttgttg gggaagataa cacggactgt ttttacggtg atcaaggaat ttttccttat
    33781 cgagaaaatg tgattactga accaaatttt tattttcgaa taccttattt tcatcaaatc
    33841 gatcgattca ctattccaga atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa
    33901 agatttgcaa acggggacaa agcaaatgac cccgaacaca ctttaccttg gggaagtaaa
    33961 gatccagacc gagaagactt tttcggggga gatttgcaag gtgtgatgga tcatttggat
    34021 tacttgacgg accttggcat caatggcatc tatttttgtc ccatatttaa agccacatcc
    34081 aatcataaat acgatacgat tgattattat gaggtggatc ccgcttttgg cgataaaaaa
    34141 cttttaaaaa acttgattga tgaagcacat aaaagaggga tacgtatcat gttcgatgct
    34201 gtattcaatc atatgggtgt ccattcacca cagtggcaag acgttctcaa aaacggagaa
    34261 aaatcgatct ataaagactg gttccacatc cgttttttcc cagtagattc ttatcagatg
    34321 actgacttac cagaaactgc tgaaaatatc ccatatgata cttttgcttt tactccattc
    34381 atgccgaaac taaatacagc aaatccagaa gtgcaaaaat atttgctgga tatcgcaact
    34441 tattggataa aagaattcga tattgatgga tggcgtcttg atgttgctaa tgaagtcgat
    34501 catcattttt ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga catctatatc
    34561 cttggagaga tttggcattc ctctcaagct tggctgcaag gggatgaatt ccatgcagtg
    34621 atgaattatg catttacaga ttcgattaaa gattattttg caaaaaagaa aatcacagct
    34681 tcacaaatgg tcagtggaat gaatcatcag caaatgcttt acagagatca ggtgaacgaa
    34741 ggaacattca acttattaga ttctcatgat actgccagga tcttgaccct ttgccaaggt
    34801 aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt
    34861 atctattacg ggacagaaat agggatgact ggtgaagatg acccggattg ccgcaaatgt
    34921 atgatatggg agaaggagga acaagatcta gagctattcg gttttataaa agaacttgtt
    34981 tctctaagga aacagttgtc taagattatt tctgaaggtt ctactcagtg gctaatagta
    35041 aatgatcgtg aagataagct ctatttcaca cggaaactag agggacagat catctatgtt
    35101 tactttaatc agtcaaaaga accttgggta gtagaacagg aaaatgaggt gattcttagt
    35161 caaaattgtc agttgttaga agacgggaaa gcagagatac gacaatacgg atttttgatc
    35221 atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc
    35281 cattataata agggtgtaac agcacgtaga atacaaagga ggaaatgaag atgaaaaaaa
    35341 gaagtgtgaa acagttgggt ttagggttaa tctctttagg aatcttaggc ggattagcgg
    35401 cttgtggcaa tggtaactct tcagcagaag gggaagccga tgaaggaaaa acgttaacag
    35461 tttccgttga ttctggatat aaagattata taaacgaaat aaaaaacact ttcgaaaaag
    35521 aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg gaatcattag
    35581 cgttagatgg gcctgctgga aagggacctg acgttatgat ggcagcatat gaccggatcg
    35641 gtgcgttagg acagcaagga catctggcgg aagtaaaact gggaaatgaa gcagcttacg
    35701 atgaaacaga caaagcccaa gtgacttatg atggaaaaat ctacggagaa cctgcagtaa
    35761 tcgagaccct tgttttgtac tataacaaag atttagtcga tacagcacca tcaacgttta
    35821 aagatttgga gaatctatcc aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa
    35881 atacaggatt tttagctaaa tggactgact tttattattc ctatggttta attgcaggtt
    35941 acggtggcta tgtatttggt gatgatggca caaatccttc agatatcggt ttgaataatg
    36001 caggtgctgt agaaggaatc tcttatgcaa cagattggtt caaaaatgtt tggccaaagg
    36061 gaatgcaaga cataaaaagt gcaggagact tcgccagcca gcaattcatg tccaataaaa
    36121 ctgcagcaat cattgacggt ccatggcaag cgcaaacata caaagaaaac aatatcaatt
    36181 atggtgtagc caaaattcct actctaaata atggacagcc ttatcaaccg tttggcggag
    36241 gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga cctctctcaa aaatggcttg
    36301 attatgtaac gaatcaggaa aatcaagaaa aattctttga aatggtaaat gaaatccctg
    36361 caaaccaaca agctagggaa acagcaaagg ctaaaaacga tgaattaaca acagctgtga
    36421 ttgaacaata tgaaacagct caagcgatgc caaacatccc agaaatgggc gaagtttgga
    36481 caggtgcaga aaacttgatg ttcgatgcag catcaggttc aaaaacacca aaagaatccg
    36541 cagatgaagc agtaaaaaca atcagtgaag ccatagaaca aaaatactga ttaaaatgat
    36601 aaccgtccat ttttataaaa aatggaacag gtgcttcaga taaaagtggc aagagcgaga
    36661 tggaaaatta tcttgctctt gttgccatta tcttaaaagc agtttgcggt ttagcaagtt
    36721 tagagggggc attcagatgc agcaagataa aacaagcaat atcaacgaaa aaaaaatcac
    36781 gctttgttca cttattccag ggatggggca gtttgtaaat ggccaagctt ttaaaggtat
    36841 cgtttttttc gccatattcg tcctattcat tggtcagatg cttctgggcg gatttcatgc
    36901 attggtagga ttggttactt tgggaagtat accaatggaa gatcattcat tatttatttt
    36961 gattgaaggc actttgcaat tcatcattac aatcattttc ttgatttttt acggtttgaa
    37021 tcttttagac gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac
    37081 gatgaaggaa gtcatacata atataggtga tgaaggtttt ccttatctat tgacacttcc
    37141 cgcttatctt ctcatgattt ttacgatcat tttcccagtc ctagttacat tgttcactgc
    37201 atttactaat tatgacttca atcatattcc accagccagt ttaattgatt gggtaggggc
    37261 taaaaacttt tttagtatct tctttttaag ctcatatcgt aatacttttt ttgcagtctt
    37321 ttcgtggacc gtgatttgga ctttatgtgc aacaactttg caaatcaccc ttggtattct
    37381 cacagcggtt gttgctcacc agaaatttat taaattcaaa cgtattttcg gtatcatttt
    37441 cttattacca tgggcggtcc cagcttttat tactatcatg agtttttcga atatattcaa
    37501 cgatagcgta ggtgccatca atactcaagt gattcctttc ataaaccagc ttccttttgt
    37561 agatatcagt gcaatctctt ggaaaacgga cccgaactgg acgaaagtag caattatctt
    37621 agtgcagggc tggttaggat ttccctatat ctatgtgatg gttacaggaa tcttacaatc
    37681 gatttcagaa gacttatatg aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt
    37741 ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg ccaacattta ttacacagta
    37801 tacggggaat ttcaataatt tctccatgat ctatctgttt aacaatggcg gtcccggaag
    37861 cgtaggcgga ggagctggtt ctactgatat tcttatttcc tggatctata aacttacgac
    37921 tggaacttct ccgcagtatt ctatggcttc agcaatcacg ttgattatct ccgtcatcgt
    37981 tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca
    38041 atgaaaaaat ttcattcgag tgctcgattc caaaaacgac tttctcactt ttttacttac
    38101 ttttttcttt taatactgtc agtcattatc atctatccgc ttttgatcac agcaagtact
    38161 gccttcaagt caggaaatat tgcagctttt agtttagatc tttcaagtga ctggacttta
    38221 ggaaacttta ctcggctatt cagtgaaaca ctttacggga cttggtataa aaatacattg
    38281 atcatttctg tcctaacaat gatcgtacaa gtgactgtag taactttggc gggctatact
    38341 tatagtcgct atcgttttat cggccgcaaa tcaagtttga tgttcttttt agtgattcag
    38401 atggtaccga ctatggcagc attgactgcc ttttatgtca tggctttctt attagaagcg
    38461 ctcgataagt actggttctt gacaatgatc tatataggcg gtggaattcc aatgaatact
    38521 tggctgatga aaggatactt tgacactgta ccaatagatt tagatgaatc tgctaaactt
    38581 gatggggcag ggcatttcag gatttttttc cagattgtat taccgcttgt caaaccgatg
    38641 ctcgccgttc aagcattatg ggcttttatg ggaccttttg gggatttcat gttagctaag
    38701 ttcctgcttc gaacacctga aaatcagact gttgctgtcg gcttacaaac atttatttct
    38761 gatgtgacaa accaaagagt ctctcttttt gctgctgggg caattttgat tgcactaccg
    38821 atttgtctat tattcttctt tttacaaaag aactttgtta ccggcctgac atctggcgga
    38881 acaaaaggat aggagcagag tatcatgcaa acatttccgt taaactattt ttccgcatta
    38941 cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat catggcctaa atttttcttg
    39001 atatttgtat ttttagtttc cttgatggtc atgcctgtga ctctttttta tgccaatcag
    39061 atccaagcga ttccattaga acaatttcta tccgttcatt cgctgatcga tgaacaagga
    39121 acccaaaaat tttctgaact tgaattatct gaaactgggt tacaagcatc gcaacaaacg
    39181 atagctgtga cccctgaaat tttggtaggt gtgtctttat ctgagaaaca acaatccgat
    39241 catggaactt ttattgattt tgagaaggaa caatgggtga tccaacaaaa ggataaatct
    39301 ggtatacgcc gctatacgat gaattatagc ccatctttcc aaccagattc tgtacgtacg
    39361 cccgaagatt tccaacgatt tttagaaaga gaattttatg ccagtaatcg tccaacaatc
    39421 attttatctt atagtttaag tcttggtctt gtcctatttg tcatgacgag tctcatttta
    39481 ttcggggctt cctttttctt gtggatgaca agaaaaagcc aactttcatc cattcataca
    39541 ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa taggttccat ggcagcagca
    39601 gttgtagggt ttatccactt tgactttatc ttgatgctaa gtgtccaaac aacgataact
    39661 gtcttgctgc ttttatgggt ttttgtcaaa acaagattca aagataagcg tgtcgaatga
    39721 caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt
    39781 gaaaaatttc tgaaaaaatt aaacttctta gttgattctt ttctttttta gtattaacgt
    39841 aatagggtag tttttttaag gaggaaaatc atgttagaga aaactttata caatcaatta
    39901 ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa
    39961 tacggagata cggacactcc tacccaaatc aaaatcaata tccatgaaga aatcccaatg
    40021 aaagagatta caagtaacgc atctttggca ctaggagaag cctatatgga tcatcgaatc
    40081 gagatcgagg gaagtatcca agcattgatc aacgatgctt atcagcaagc aaacagcttt
    40141 ttacgaggaa aagattatct aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa
    40201 aacaaagaag atatccatgc gcattatgac ttaggtaacg acttttataa actttggcta
    40261 gatccaactt tgacttattc ttgtgcttat ttcaatacac ctgaagatac gttggaacaa
    40321 gcacaaatca ataaagtcca tcatatctta gacaaactct ttatcaaaga aggcgaaacg
    40381 ttgcttgata tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa agagtataat
    40441 gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg atttgattac caaaagaata
    40501 aaagaagaac atttagaaga taaatgccgt gtattattaa tggattaccg cgaactaaaa
    40561 ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat
    40621 cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac caaaaggaac agctttgatc
    40681 catggaatca gccgacaaca aggcggtgcg acaaatgctt ggatcaataa atatattttc
    40741 cctggcggtt atgtccctgg tgttactgaa ttagttgata atattacaaa aaacgatttg
    40801 caactgattg atttggaaag cttacgtcgt gactaccaat tgacattaga acattggaca
    40861 aagaattttc atcaagtcca agatcaagtc acgaaagaaa tgggtgattc cttctatcga
    40921 atgtgggatc tatatttaca agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg
    40981 attcaatatt tgcttgttca acctggaaac aatgacatcc caatgcatcg tgcataattg
    41041 aggcaaaagt actgtttaaa gactatttaa aaagaagcta agttttatga tgctccagaa
    41101 gtgaactttt ttgtctaact tctaggattg tctctaaagc ttagtttctt tttctttata
    41161 ttttcataat aaaagtttgg ttctttaatt tttaaacatg acagaaaatt ttttcttcgt
    41221 tatacttgta atagaatact attttataac aaaggggatc gaatcaatga aaaaaactgt
    41281 tatttatact agtttatttg cagctttgtt tttagctgct tgtcaatcaa ataccaatca
    41341 aggcacatct gggaatacgt cttccagtag cgaacagcgc tcttcacagg tatcggactc
    41401 ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa agttctagtg aagcaagtac
    41461 tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc
    41521 agatactgca attactagtt tggaattaga tagtgattgg ggatcttact tctacaagat
    41581 agaaggtgtc gatgatcaaa aagaatacga agcaagaatt gatgctcaaa atgaaaaaat
    41641 ggagacagag aatccagaaa ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga
    41701 agatggtctg gatgttgata aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga
    41761 caaagtagga agcggtacgg caacagattg ggatttggat aaagatttag gtactactta
    41821 ttgggaagtc aaagtaaaag acggcaatca aagtacatca gtcaagatca atagccaaac
    41881 aggcgaagta ctgtcaactg aacaagatga ttaagaattt cttcggaaaa caagagttga
    41941 tttttctttt agtcatgata cactagacaa aataacttgt ttgaaggagc gatttacttg
    42001 agtttagaaa cgacctttgc caatcatatc tttgccaatc ctttgatgaa tgcatctggt
    42061 gtccactgca tgactaccca ggaattagat gaactggctc attcagaagc aggagcattt
    42121 atcactaaga gctgtacgat caatgaacga aaagtaaatc ccgaaccacg ctattttgat
    42181 gttcctttag ggagtatcaa ttctatggga cttccaaact taggtttttc ttattatcta
    42241 gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc
    42301 attgccggga tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg
    42361 tttaatggga tcactgaatt aaatttatct tgtcccaatg tgcctggaaa accacaacta
    42421 gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag tattcagcat cttttctaag
    42481 ccgctaggaa tcaaattgcc tccttatttt gattttgctc attttgatca gatggctgat
    42541 atcttgaatc agtttccatt gacctatgtc aatgctatca acagtgtggg caatggtttg
    42601 tatatcgata cagaacaaga agcagtagtc atcaaaccaa aagaagggtt cggcggcatt
    42661 ggcggagagt atatcaagcc gactgctttg gcgaatgttc gcgcttttta tacacgtttg
    42721 aaaccagaaa tccaaatcat tggtacaggt gggattcgga caggacaaga tgcatttgaa
    42781 catctgctat gtggcgcaag catgttacag attggtaccg agcttcataa agaagggccg
    42841 gaaatttttt ctagaatcat aaaagaatta acgcagatta tgtcagaaaa aggatatacg
    42901 tcaattgacg aatttaaagg caaattacgc accatttcat agcaaagaga cgaaggtcat
    42961 gattgatctt cgtctttttt tataaaaaac actcgtctgt tcattcctgt gcaagcgtac
    43021 tcattgtatg ttagacgaac ttgtgggaaa ataacagagg caaaaaggag gataatatga
    43081 atgatgcaac agaattagca aaagagctta aagaaaaaga atttagtttt caagaactag
    43141 ttacggatat acagaaaaaa ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc
    43201 ttgaaccatt cgagagtaat tcccacgagc tattgattcc ccagtcagaa aaagggccat
    43261 tggctggtat cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta
    43321 cttcaggatc tcgtttattt gaaacacacc gagcttctca tacatctaat tatgtgcggc
    43381 aagcagaagc gataggtcta gttccttttg gacaaaccaa tgcgccagag tttggtttca
    43441 aaaatattac agacccagtc atctatggcc cagcaagaaa cccttggaac cttgaatgta
    43501 caccgggcgg atcaagtgga ggagctgctg ctgcagtttc atcaggaatc gttcctttag
    43561 ctggtgctag tgatggcggt ggttctatta gaatacccgc ttcgttttgc ggattgattg
    43621 ggctgaaacc ttcgcgaggg acgatgccag ttggtccata tgcttggaga ggatggcaag
    43681 gagctgctat tgatttcggc ttgacagttt ctatgagaga cacggaggct ttattttatg
    43741 ggatgagaag tatccattcc ggtgcgccat atcaagtatc gccagttgag tggcaaacac
    43801 atccaagaaa aaaacggttg aagatagctt tttgtgttga ttcaccgatc cactcacaga
    43861 ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga tttccttgga aaagaagggc
    43921 atgatttgac agagattcct tacccactag atggtcgtaa actgatcacg tcttattatc
    43981 agatgaatgg cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc atccagcgag
    44041 ctgtcagaaa agaagaactt gaacccattt cttggacttt gttccaatat ggtcagaagc
    44101 tctctgccgc tacttacatc gattctctgc atgtatggga tgaagcagca attgtcatgg
    44161 aaaacctaca tcaagaatac gatctattcc tttcaccaac gactgcattt accgccccta
    44221 aaatcagcga agatttgcaa agtgatgcga taagagcaca gatggaacac atcagtgatt
    44281 gttctgaagt agaaggttta gacgttgttt cttccatgtt tgaggagagc ttgaagatca
    44341 caccttatac acagttggct aatctgactg gacaaccagc aatcagcttg cctactcatt
    44401 tgacaaaaga aaacatgcct ctaggaatcc aatttatggc tgccagagga agagaagatt
    44461 tgctatttca aatcggctat ctatttgaag caaatcatca gttccattta ccaaatccat
    44521 ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa agattgtttg ctctaataac
    44581 tagtgccttt tttattttaa gcgcttgcgg tacagctgcc gctaatcagg aagataacag
    44641 ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg acttctggca cactgtttcc
    44701 cgcatcttat tataatgatc aaaatgaatt agttggttat gatgtagatt tggcaaaaga
    44761 ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat
    44821 cacttctgtt tcaaaaagag aatcagattt tgctgcgaac gattttggta tcacaaaaga
    44881 aagagaaaaa aaattctccc tttcagagac tttcaaatat tcatttgaca gtatgatcgt
    44941 acgaaaaaaa gatgattcag gaatccactc acttgaagat cttaaaggga aaaaagcagc
    45001 tggcgaaccc aatactagct atatgcgttt agcggaaaaa cacggagcta aattagtcac
    45061 atatgacaat gccacgaatg atcagtattt aagcgatgta gcaaatggtc gaacagatgt
    45121 tattctaaat gattattatc tgcagaaaat ggccgtatct gctttaccag atatccctgt
    45181 aaaaatactg gagaacgttt atttcaatga aaacgaaagt ggttttttat tgaaagaagg
    45241 aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag aagatgggac
    45301 gacaaagaaa atatctgaaa aatattttca aactgatgtt tcagtcaaac cgaaagaaac
    45361 gatggctgag tcggtgagtg agtgatgtac tttcctctaa ttgatttttc cctcatgtgg
    45421 acttcacttc ctttcgtact gcaaggcttg gtatacacat tagggattgg cttcgtgtcg
    45481 tttcttttag gaaatctggt agggttgctt ttgacagttt taggtctttt ggattggctg
    45541 cctctgaatg tatttattcg tttttattta tcgttcttta gaggcattcc cgcactagtc
    45601 ctgctgtttc tgttatattt tgggttacct tatcaattaa gtgccttaac ggcttcagtc
    45661 atctgcttta cgattaccag cagtgcattt attggtgaaa tctatcgagg atcactagca
    45721 ggcgtttctt caggtcagtg ggacgctggt ttagcttctg gtttttcttt ctttggggta
    45781 ttgaaatata tcgttttgcc gcaagctttt cgaatctcag taccggcact cagtaacgta
    45841 gctatggatc tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc agaaatattt
    45901 caaaaagcaa agatcatagg cggtcggaca ttcgattatt tttctctcta tattttagtt
    45961 gcactgattt attgggtcat ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa
    46021 cgttctatgc atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt
    46081 ctattttttg tttcttgtat attctgccta gtattttaaa accatttcat gtaaaatgta
    46141 aatcgaaaat atgttcggag gtagaaaaca tgaagacccc gctaaccatc gaaatagaat
    46201 cctcgttata ccaatattgc atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc
    46261 ctgatgacca aggaattgta gatacgctta gctgcttgac aaaatttgat ggaagccgag
    46321 aatggcgatg ctatgagctg aaagtgacaa aagcagattt tcgctctaca gccaaactgt
    46381 catttgttgg acattataat tattttgttt taccaagtaa actatatgaa gaagtaaaaa
    46441 ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga
    46501 tgcctgcctc tggtacattt ctgattgcga aaaaagcagc aaagaaagag cttgctgtac
    46561 cggaagaagc tttgacaaac cgcttcatgg cttctttatt cagggaagta agaaaagcaa
    46621 agcaaatgga gcgaggcgta aaatattttc caacagatca attgtacaaa gaattgaaaa
    46681 gacgttcaga aaatcaagat ccattttcta acgaacgata ttatgaacgg tttttagagg
    46741 aaatagctag tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt cagcatgatt
    46801 atgaatattt gaggcaacaa cagcagatcc gtcggttgcc tacagagcca ttggagtgaa
    46861 aaatatgtac tatccttatt taagaggaaa acaatttgac ctattagcat taaaagaagc
    46921 actgtcgcga ggattgctct ccaataaaat ccaaccagtg atcgaaccag tacgtgattc
    46981 cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag caccatcgaa tcgctgtgat
    47041 ccaaaatccc caagtaggac aattcaaatt attcgatcag catgtgcaca gttggacagt
    47101 caaagaaaat agctctgtcg tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt
    47161 actggattca cctccagcgt ttcttgtttt cgatggacag cactacccaa aagatacaga
    47221 ggtgtggaaa cagttggcag gagttgattc gaaatttttg ataccagata ctagtcgctt
    47281 tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa cgagaaagca
    47341 tgttgaaagt tatgcagata aaacagatga cttttttagt gatgactatc tttttttcca
    47401 cgcagacgga tatattggat tttctgattt cacgatcgaa ggtagccgtt atttcgataa
    47461 agggttccct agccgagcgc ttgctatcca tctaacgtat attgatgctt acggcaatat
    47521 acgcgtgaaa cattttgtct ctgacagtaa cgactctgct aaagaccaag cattgaagtt
    47581 tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc aactcttgat
    47641 tacttcagga atgattgaat tattggctct ttaccagcaa caaaaattcc ctggactggg
    47701 agtgttgaaa aagtggtcac ttatgcatca tttggaactt attagtcacc tgctagatca
    47761 tccaaaagat tggcttaaat attattcaaa agttcaggaa ctatatgaag tgatccaatg
    47821 attagataat gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat attcggcaaa
    47881 caaggaaaga agaagtagaa gaagtggtag aaatcattaa tgccgcagcg gatttcattg
    47941 cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa acgattttga
    48001 acgatattga aagaaaagaa agctatgtat taatagtgga agatcaaatt gctggaacag
    48061 ctgcattagt ctcaagcgtg gatcccgttt acacaaaaat agatggtagt tggaaagaat
    48121 caactggtcc ttatttatcg attcatcgtg tcgctttagc agaaagtact cgaggaaaaa
    48181 aactagcaaa accgttctta gaacatttgt tgacgattgc agccagcaaa aatatccaag
    48241 atatccgtat cgatacttat ccagctaatg aaccgatgga aaagaccatc tattctgctg
    48301 gattcactta tcaagggatg attgaatttc cttttgaaca cggcgaaaga aaagcatatc
    48361 aaataatcat caattgacta taaaatcatc tgagcaaaga taaagacatt aacactactg
    48421 aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca
    48481 agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa
    48541 tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac atagcttttg tcacgacctc
    48601 tcggcttatc ctacgagtat tattcgatac atttatatca ataatccttt ttttatttta
    48661 tctgcagctt cttggaacgc tccatcgttt gacacgacta aaagtgaggg gatagctttc
    48721 aaacgtttca actgtgctaa atcttctaca taaaccgcca gtctggctga aatctgttca
    48781 gatgaatctc cacgtttttt caggcgcgat tgcaatgttt ctttagagat cgtaataaag
    48841 ataggacgga ctaccgtccc gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa
    48901 acggcgtaac aatttcctac tgtcagtttc tttttaattt ctgctttact ggaaccatat
    48961 ttatgttgag catattggtc ccactctgca aactcatttc ccttgatcat ctgattgaat
    49021 gtattgtcag aaacaaaata ataatcctct tgatcttttt ccccaggtct tggtgatcga
    49081 ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag cttctgcgag tgaagttttt
    49141 ccagaaccag aaggaccgat aaatacatag caataattca ttgtcatttc tcctttgatg
    49201 attgaacaag tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt aactttgctt
    49261 ttttgtatat tataccagca agccagtcac aatagcactt aaaaagctga ctaaagtcgc
    49321 tgttagcaaa attttcaatc cgtgttttgc gacaagattt cctttttctt catttaatcc
    49381 cttcatcgca ccagaaatga tcccgataga agagaagttg gcaaatgaaa cgaggaagac
    49441 agatataatt gctgttgtac gctcagtaaa atggaaatta ccatttgcta attctgtcat
    49501 agcaacaaat tcatttgtaa ccagtttagt cgccatgata ctaccggcat ccactgcttc
    49561 tttccatggg attccgctga tgaaggcaag aggggcaaag atgtaaccta ggatctcttg
    49621 gaaagtgatg ccgaatatcc cgtggaagat agcattgatc atggcaatca aagcaacaaa
    49681 accgataagc atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc
    49741 caacatttgg aaaaatgtct gttctttgtt ttcttcaatg atcagctcat cttctttttc
    49801 ttctaataca tagggattta caattgaagc cagaatataa ccgccaaata agttcaaaac
    49861 aagggcagtg atcacatatt tagaatcgat cagtgccata taagatccga cgatcgacat
    49921 agaaactgta gacatagccg atacactcat ggtgaacaaa cgtttctcag ataggaaggg
    49981 taattccttt tttatggaaa taaaaacttc cgattgtcct aaaatagcag aagctactcc
    50041 attataagat tccaatttgc ccatcccgtt gattttactt aatcctagac caacagcttt
    50101 cataaataat ggaagaatct tgatatagcg tagaataccg ataatagcag aaataaatac
    50161 aattggcatc aatacggaaa gaaagaaagg tgcacttcct ttatttgcta caccggcaaa
    50221 aacaaagtca acaccttgcc cagcaaaagc aagaagatga tcaaacattt ttgccaatac
    50281 agaaacaacc gctgtaccaa aagttgtacg taataatacg aaaccaagaa caagctgtaa
    50341 aacgaacatc acaattagtg gtttcaattt gattgctttt ttgttactgc tgatcaaaaa
    50401 agcaagccct aatgtgacaa ataaaccaat aatacctaat aaataactca aataatcact
    50461 cctattccaa attccgtttt attttacctg ttttgcaaat aaaatactaa tctttagtga
    50521 aattttcatt acttttttag agtgaaacgc attcattttc cattagaaaa taaagcgaac
    50581 aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc
    50641 gattaagcta atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata
    50701 gattttcgga agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt
    50761 gttcatcatt tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta
    50821 gtttatcata agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca
    50881 agacttcatt gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt
    50941 gtattgtttc aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat
    51001 gttttactgc atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg
    51061 taatgatgac ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac
    51121 tttgcgcttg aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag
    51181 gacgtaaacc gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca
    51241 atcctttttt gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg
    51301 cttcttggac aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag
    51361 taggagattg ataaacagat tctttaccag taattttttc aataatacgt ttgattacag
    51421 gaaatgtctc aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga
    51481 aggaatcgat catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca
    51541 gcggatgttt caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg
    51601 catgtccttt ttggctttca tgatacagtt ggttcaagga agtagcgagc tttccacttc
    51661 ctgggccagg agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc
    51721 caaaaccatc ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt
    51781 gtttatagac cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct
    51841 gtccactata gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat
    51901 cgattaaccg caagatatcc atatcataag taatcccata atcaccacgg atcttattac
    51961 gttcaatatc tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca
    52021 gcaatttgat tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt
    52081 caactagctt cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa
    52141 tatacgctga ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt
    52201 tccctcctta agtactaaag cattttttca tttttttgag aattcgtcaa ctggaagagc
    52261 gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat
    52321 tggtatgata gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac
    52381 gcatataaac actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc
    52441 gaattggaac aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag
    52501 catcagcgca ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg
    52561 tcatcggatt cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt
    52621 accaaatttt ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt
    52681 ttacagaaaa tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc
    52741 aagtagtatg aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac
    52801 gaatgcggat caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc
    52861 aagcatgaat ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga
    52921 agaagatatc cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag
    52981 aagctcaaat gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt
    53041 tttacaggaa tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta
    53101 caaaatcggc acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga
    53161 aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac
    53221 ggactatgac aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat
    53281 tgaacgttta gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa
    53341 taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa
    53401 gcaagtacta tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat
    53461 ccagcagcaa aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat
    53521 cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca
    53581 agcaatccat gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa
    53641 tgaaggaggt tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac
    53701 aaacagaaga tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg
    53761 atgacacaga agaaatccct atgctagagg aaacaaaaat acacgaaaaa gaaaaagtaa
    53821 ctccgacgaa tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg
    53881 ggtatttaga aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg
    53941 aagcagaatt agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat
    54001 tattagaatc atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga
    54061 caagcctgat cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg
    54121 aacggtcaaa ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt
    54181 tgaatggaca aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc
    54241 aagtggaatc gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag
    54301 atgaccgggt gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc
    54361 agatcacgat attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt
    54421 ttaccgttaa tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg
    54481 aatttgaatt gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat
    54541 cctacgatat ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt
    54601 ctttaacgat aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact
    54661 acccacgatc tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga
    54721 gaaagtcctg attggataag ttggattacc agatgtcatt cagataattc tgttccaaag
    54781 gacttgcggc agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac
    54841 gtgaataact tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac
    54901 agcttagctt attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga
    54961 atttccagcc aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg
    55021 cgttaattgt catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat
    55081 gggtcatcgg ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct
    55141 tgagaaaaac agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca
    55201 tggtttacgg aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt
    55261 ggatctatta ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt
    55321 tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg
    55381 aaaaaaataa tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc
    55441 agtacaacct atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa
    55501 gcaacaatca cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt gttcttcaaa
    55561 aaaggcgatg atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc
    55621 ttcatcactt cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt
    55681 actgtggacg ggaaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa
    55741 tcggtcattt tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata
    55801 ttgccataat tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa
    55861 aaaagcggaa tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc
    55921 aaatttgtcg gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt
    55981 atcgttgatt gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga
    56041 cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc
    56101 gcaatgatgc ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg
    56161 acgcatccat cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt
    56221 ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact
    56281 gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt
    56341 tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg
    56401 acttatgcaa ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg
    56461 ctgatgggag ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc
    56521 gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg
    56581 ctgatcgata taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca
    56641 gatatcgttg gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat
    56701 atgcgtcagt ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac
    56761 ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata
    56821 taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc
    56881 tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg
    56941 cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct
    57001 tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt
    57061 ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca
    57121 tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt attaggagca
    57181 agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca
    57241 ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat
    57301 aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg
    57361 atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg
    57421 acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca
    57481 ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga
    57541 ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa
    57601 aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac
    57661 ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg
    57721 tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa
    57781 agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt
    57841 agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat
    57901 agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt
    57961 tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta
    58021 aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag
    58081 tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc
    58141 aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata
    58201 atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt
    58261 gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt
    58321 ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac
    58381 attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt
    58441 tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg
    58501 tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc
    58561 actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa
    58621 tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc
    58681 aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat
    58741 cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac
    58801 tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt
    58861 agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga
    58921 ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat
    58981 tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga
    59041 ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact
    59101 ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc
    59161 catggtagga acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga
    59221 tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct
    59281 tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta
    59341 tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt
    59401 attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt
    59461 aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg
    59521 cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta
    59581 tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt
    59641 aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct
    59701 gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg
    59761 aagtattggc ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt
    59821 gaaaaatgtt aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt
    59881 cacttgaaaa acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag
    59941 tttcatatct aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga
    60001 ggaaattaaa cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg
    60061 tatcaagaac catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat
    60121 ttataacgaa ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa
    60181 agattttcct actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt
    60241 gaaagagaaa gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt
    60301 tgtcaacaca ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga
    60361 ctctaagatc actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga
    60421 tgaaggtcag tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta
    60481 cgcttatctg attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg
    60541 tgcagcgctg attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa
    60601 aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat
    60661 tattggtaga agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa
    60721 taatttagaa gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga
    60781 aatcgaaatt ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg
    60841 cattgccatt tcagcgcaat aattcctttt attcaaaaaa cataccaaaa atattccaac
    60901 atcatcttgg actttctaca tgttactaat gagcgtgtaa aagaacgaga tggtgttttt
    60961 atttctatca atgaagctct ttttctttgc aatcctcact tcaatcctct ataatgtcgc
    61021 atatagttta gtaaatgagt aaactactaa tttacttatt tactaaaaat aaaaggatga
    61081 ggagttaaaa taatggaaat accaacaaat ttaaaacata aaccagtaat cgttgtagaa
    61141 gactatgata aagttgatgg aagaaatgca ttaaatacgg atgcaaaagg gctttcactg
    61201 ggccttgctc agtggaatga tcgtggaaaa gtcgatatct caggcaaggt atggcgacat
    61261 actggagaaa aatggtcacg acagtcggaa gaattaccaa tcacacgtat tttagattta
    61321 gcgatcttag ctgcacaagg aagtttatac ttccaagatg cttatcgtta tgaaaaattt
    61381 tatgatccag aaaatccagt cgtagacatc attggattac aagggaaccg aatgacggta
    61441 gaagtagata ccaaaaatcc aaagatcgat gaagacatca ttttgtatta cgatacacta
    61501 caaaaagacg gagaactgat tggggaacga ttccgtattt taaaacgtct tttagatgag
    61561 ctaggatatt aatagacaga gatgatcaaa aggggaagag aagcatggag aatttctcac
    61621 taacagaaaa ccgaaccttt tcacggctag aaaagcaaat gatctggaag aaaccgaaaa
    61681 gccatcggac aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg aattggcaag
    61741 atcctgaaat gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg gggaagacac
    61801 aattagccaa agcactatct gctgatctgc aacttcccta tacaaaagtg acctgttttg
    61861 cagatatgga caaatcagat gttttcggag ccttattacc tgtagtagat tctaataagg
    61921 aacaagacca agaattacta gaagctattt accaaacaga tacacttgaa gctgtactga
    61981 cactgatcca gaaccattac ggcattgatc ctttttctgc taaagaaaaa ttagcagagc
    62041 tagttcaaag gatcgaagct gattatccag atgcaatcca ttacagctat tacccttctg
    62101 aaatcgttcg agcgattgaa aaaggatatt tattagagat tcaagagccg actgtcatcc
    62161 gagatgcctc tgttcttgtg gcacttaact cagcgctcga accaaatggg atgttgaatt
    62221 taccgacagg aatcattcgt cgccatccag attgcgtcat tgtgatcact acaaatcgaa
    62281 attaccaagg aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa catgcggaaa
    62341 aaatggatct tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa actatgatcc
    62401 aaaaacaaga acttctctta aagatggcag agattatccg tttgttggat gaaactgcaa
    62461 aagctaacgc catcaaaggt gtagcaggaa tgcgttctta tttttattgg gcaaatacct
    62521 ataagcaagg acaagattta ctccagtcga tttatccaaa agttttgtat aaattatcca
    62581 ctgattcaga tgaattggca attcttgaac aagctttgtc tgacagtgga ttgctactgg
    62641 aacttcagga catattgaga aaagaaaaat ggggtcatcc tcaatctgag catacaaaag
    62701 gaagaaccat tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat gctgaagaaa
    62761 ttctgcgctt agatgaaaag aaatcgttgt ctgctgaaga accaaatgag ggagaagaga
    62821 aacaagaaga aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa gaacgcagta
    62881 gcgatatcga aggaaaaaat caaaaagaac aacaaacagg agatgattct caagaaatgt
    62941 ctgcttctga catggaagca catgataagg aaatcaagaa gcaactgaat aaagaagcgc
    63001 gacaaatcat gaaggaaacg attcatgaaa aagaaggtct catcgttcac cgaccagctt
    63061 tccaaggaaa aacgccggaa gcacgtgagc tacacatgca agtaatgcca atagtagaat
    63121 cacttagccg gcaaatttta gagttactgg ataatgaaca aacagagacg taccaaaaag
    63181 gaaaatacga aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat ttgagaaatt
    63241 ttgacaaaaa gaacccgcca catgagcaac cttctttagc ggtagctgta cggatcgatg
    63301 aatctgggtc aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca gcgattgcca
    63361 ttgccgaatt ttctaagaaa gtcgatatcc cattgttgat ttacggagat acagcagacc
    63421 gttcgtcaag agaaaagact tctttgtttt cttataaaga atttgaagac ggatttcaat
    63481 ggttaaatga aaaattagtc actatgaaac caaggcaaaa caatcgtgat ggtgctgcac
    63541 ttcgtttagc agcagaaaaa ttaaacagac aatccgcaac aaccaaactg ttgcttaata
    63601 ttagtgacgg tcagccaaaa gcgttgccgg attatacagg caaaaaagca aaagaagata
    63661 tccagtctgt tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc gcagctattg
    63721 gccaagacaa agaagaaatc aagtcgatct acggtgaaag tcgttttgta gatattactg
    63781 acttgaatga atttcccaag caaatgattc aactgattgc ccgctatcta tgataaaata
    63841 aagctcaacg aacgcactta gcagattcgt tgagcttttc gtttctagtt accggtaaca
    63901 aggatacttc taaaatagaa tcggttataa tcaagaggga ccgttaaagg agtgaaacga
    63961 tatgatacaa gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct atcctgctag
    64021 tttcaaagat agcaatggcg atggtcaagg agacatcaat gggatccgtg aaaaacttaa
    64081 ttatttgaaa gatcttggta tcggctttat ctggatcaat ccgatctatc aatctccatt
    64141 tgtcgataat ggttatgacg taagtgacta tcaagatata gaagagagat tcggaacgat
    64201 ggaagatttt gatttattgc taaaagaagc acatgaatta gggatcaaag tgattatgga
    64261 tcttgtcatc aaccatagtt cggatcagca tcaatggttt gaagaatcga aaaaatctaa
    64321 agaaagcccg tatcgcgatt attacatttg ggtcgacggg gtagatggca aagaaccgaa
    64381 taattggaca tcgattttcg gcggttctgc ttgggagtat tctcatgaaa ctggacagta
    64441 ttatctgcat gtttttgcaa aagaacaacc agacttaaat tgggaaagcg agaaattgaa
    64501 agaagaacta ttcaatatga tccgatggtg gttggataaa gggatagatg gatttcgttt
    64561 agatgcgatt tctcatgtaa aaaaagatga atattcagta aaagccacag agaacccttt
    64621 ctcacccttt caaaatgtct caggaatcga agaacatttg accgatttga aacatgtttt
    64681 tgaagagtac gatatcatga ctgtcggaga agcaagtggt gtcactgcag aagaaggacc
    64741 acaatgggta gggaaagacg gttatttcga tatgatattc gaatttgatc atatccatat
    64801 ctggcagcaa gaaaaagaag ggcaattaga tgtccttaaa ttgaaacttg cattaagcgc
    64861 ttggcaaact tcattggatg gtatcggatg gaacgcttta tatatggaaa accatgatgt
    64921 tcctcgtgcc gtttcagtgt ttggagatac tcgtcccgat ttttgggcta tgtctgcaaa
    64981 agcaatcgct atgatgtatt tctttttgca aggtacgcca tttatttatc aaggacagga
    65041 aatcggaatg acgaatatgc cctttgaatc gatcgatcaa gttgatgcag tagattcgaa
    65101 aagactttat aaaaggctgc ttgcggaagg taagacaaga gaagaagcat tggatatcat
    65161 tcgtgaaaca acgagagaca acagcaggac acccatgcaa tggacttcgg aacaatatgc
    65221 tggtttttct acacatgaac cttggctgat cactaatcca aataccaaaa cgatcaatgt
    65281 agaacaacaa gaatatgaac cagagtcggt cctgcaattc tataagaaca tgatccgcat
    65341 tagacaaacc aataaaggtc tgatatacgg ttcttataaa gaatatcttc atgaacatcc
    65401 acaactttat gtctatgaaa gatatttaga agatgaacaa tatcttatta tggtaaactt
    65461 aacagaatca cttgctgact atgaacttcc aaaagaagca gatcagtcct ggacattact
    65521 cttgtcaaat tcctctagcg gagaatttga agctaaaggc atactagcac cttatgaagc
    65581 tcggttatat aaaacaaaca agtaaaactt cctcgttaaa gttcattagt tggactttgc
    65641 gaggaagttt ttcagattgc tctgtcaatt actcatttca ataattcgct ctaggaccac
    65701 cgatatattt aggacgagag cgaagggctg tcacgtgagc ttgtccaagg gttttcaaac
    65761 gaggtctgac aggtacttgg acatgtttga tatgcatacc gattgatgtg tcaccgatat
    65821 cgataccggc ttgtccaact acgtgttcca cttcgacagg atcattgaac tggtcaaaag
    65881 cagcaacaga acaagcacct ccagcatgta gtgcaggaac aacagagaca atttcgaaat
    65941 tttttgcttc tgccactgtt cgttctacta ctaatgctcg gttcaaatgt tcacaacctt
    66001 ggacagctag cgcaatttct ttaggatcca acaactcttt taacgtacgg atgatccatt
    66061 gaccaacttc cgcactggaa ttttttccaa tatgtcctcc aacgacttcg cttgtgctgc
    66121 atcctaaaac aaaaagatca ccttttttca agtgtgcctc agccaatatg tcattaacta
    66181 tttcagtcaa ttggtctttt aattcttttt ctgacaactt cattttctaa aaccttcttt
    66241 ttcaattaat attgcttccg tgcttgaata cgttgaagac ttgcagttat aggaaaggca
    66301 agtcccattc caatcagatt ttggaagatg tttcctggta tagaagccac tccagccggc
    66361 caatcatata aaatccaacc ggcgatacca tagccgatga tcataactaa gcttcctaat
    66421 aaaagcccta atccccacat acgagtagaa gatttgctga aagaaccagc aacatatcct
    66481 tgtaacccgt ggatcactaa tgaaaaaact gcccactgcg gatatcctga aatcaaatcg
    66541 atcaatagac cactggaagc accaacaact aatccacccg ttggaccata aagaaaagca
    66601 gcggtataga tcccggcctc gcataatgtc acaaatccat tcgtagccgg tacagggatc
    66661 aaaattgtta atgaaagggc aactgtcaac gcaatcagca tagcatagat cgttaattgc
    66721 ttagttttca tttctcattc ctcctttttg aataccgcca gaccagacac ttccggtatg
    66781 atcgttcatc agcacgccat tagaaataca atgggcaaca aattttttac tttctgctac
    66841 tgctttggta aaatctgccc ctaacacaag attcgctgtt attgcagaag aaaaggcaca
    66901 accggccccg tttactgttg tagtgtcgat ttttttatga gaaaatacct cataatgatc
    66961 aattgaatag agaagatcca cagcttcatc tccagccatc cttgtgccgc ctttgatcac
    67021 aatgtttttt gttcctgaag cagtgagttc tttagcgaga tggatcattt ctgttaacga
    67081 agagggaaga gcttgaccag aaagtaaagc agcttctgcc aaattaggcg taatcatcgt
    67141 aacaagtgga aaaagttctt cgatgagctg cgaaatatac aattgattga tcacagcttc
    67201 agtttctttg aatgccaaaa cagggtctaa aatgattggg atattttgtt ttgtttttat
    67261 gaagtctcga acaaccatga ttgcttcact tgaattcaat aacccaattt tgataccatc
    67321 taatgaagtc attttatcaa tcgaaactaa ctgtcgttta agcaattcca aagatataga
    67381 ttccagcata aatcgctcat taatatctag tgcccctata gaagtcaagg cgcttagtcc
    67441 gaatagacca aaattctcaa aagtttttaa atctgtttgg attcctccac cagcaaatgg
    67501 atcagaaccg ccaattgtca agattctttt cataacattt tcctccatga ataagagtat
    67561 aacgaaattt tcaaaaaaga aaaacagcca attggcatat ttttatctca tccaatttaa
    67621 aaaaacaaaa agaaaagaaa gaccaaaatg gatctttctt tccttaaata gttaggactt
    67681 ttgtgatttt aattctttct cgatttgaag ccgttgcact ttcattgtag ctgttcgcgg
    67741 gattgcatca aaatcacgga tgatagggac gttcaagcga ggaagttcat ggacttgctc
    67801 ccaccatcga ttccaatcca tttctttatc aggagctaaa gcaataatcg gttgtggaga
    67861 atgattttta tcccttacaa tcactacttc tgccaaaaaa tctaacgaat ctaataaaaa
    67921 gtcttcaata gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc gatctttcaa
    67981 aaataggtgg ccatgcttat ccataaaacc gtaatctccg ctatcccacc aatcgccata
    68041 aacattttcc tggaaccgtg catcttcctt gtaataagtc aaagcccgtc ctttagacag
    68101 taattgtata tgaccatctg tgttttcagg taggacattc ccaactgaat ctgtaatccg
    68161 agcgcttgtc atgtcttcca gtccaacgcc catatctctt gcatcacttg ttttcaaact
    68221 ctctagtgta tgagccttaa gaatcattgg tccgcattca ctttggccat atacttgaag
    68281 aaataccgca tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct ggttgtttat
    68341 agcatcaaaa gtggagtgat agtatcggat acccgaaaaa gcttctggat gctccttagc
    68401 agtaaaacgc cactgaacaa agttattcgg atgggtctct agtgcaattg gccggtgagc
    68461 ttccaacata tgaacaactt ttgaactttg tgcgtttgct aaaggcatca taggaaatcc
    68521 catggacatc aaagaagaga taccaatatt gaatctggaa tgcactggag agatgtgaaa
    68581 agcgattaat ttcttttccg aaatttttgt gaaaactgtt ttttgccatt tcgtccgcca
    68641 tcccatagaa tgagcagaat gacaaataag tttagggata ccagttgtac cagaagtatg
    68701 ggtcatataa gaaatttcat ttttgtctaa aaatgattga gaaacagaca aagcaggttg
    68761 ttccattaaa taaagaatag g
//
LOCUS       DACEMK010000004        60101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEMK010000004 DACEMK010000000
VERSION     DACEMK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.004"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(92..1111)
                     /locus_tag="IW715_000202"
     CDS             complement(92..1111)
                     /locus_tag="IW715_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1020833.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1133..2140)
                     /locus_tag="IW715_000203"
     CDS             complement(1133..2140)
                     /locus_tag="IW715_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1020834.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2133..2798)
                     /gene="pgmB"
                     /locus_tag="IW715_000204"
     CDS             complement(2133..2798)
                     /gene="pgmB"
                     /locus_tag="IW715_000204"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1020835.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2791..5085)
                     /locus_tag="IW715_000205"
     CDS             complement(2791..5085)
                     /locus_tag="IW715_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1020836.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5345..7510
                     /locus_tag="IW715_000206"
     CDS             5345..7510
                     /locus_tag="IW715_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1020837.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7814..8647
                     /locus_tag="IW715_000207"
     CDS             7814..8647
                     /locus_tag="IW715_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1020838.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8749..10449)
                     /locus_tag="IW715_000208"
     CDS             complement(8749..10449)
                     /locus_tag="IW715_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020839.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10956..12116
                     /locus_tag="IW715_000209"
     CDS             10956..12116
                     /locus_tag="IW715_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1020840.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12132..12575
                     /locus_tag="IW715_000210"
     CDS             12132..12575
                     /locus_tag="IW715_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1020841.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13058..13588)
                     /locus_tag="IW715_000211"
     CDS             complement(13058..13588)
                     /locus_tag="IW715_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1020842.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13693..14244
                     /locus_tag="IW715_000212"
     CDS             13693..14244
                     /locus_tag="IW715_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1020843.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14397..14870)
                     /locus_tag="IW715_000213"
     CDS             complement(14397..14870)
                     /locus_tag="IW715_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1020844.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15008..16480
                     /locus_tag="IW715_000214"
     CDS             15008..16480
                     /locus_tag="IW715_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1020845.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16477..17187
                     /locus_tag="IW715_000215"
     CDS             16477..17187
                     /locus_tag="IW715_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1020846.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17419..17703
                     /locus_tag="IW715_000216"
     CDS             17419..17703
                     /locus_tag="IW715_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1020847.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(17984..18403)
                     /locus_tag="IW715_000217"
     CDS             complement(17984..18403)
                     /locus_tag="IW715_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1020848.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18405..18989)
                     /locus_tag="IW715_000218"
     CDS             complement(18405..18989)
                     /locus_tag="IW715_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1020849.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19036..20511)
                     /locus_tag="IW715_000219"
     CDS             complement(19036..20511)
                     /locus_tag="IW715_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1020850.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20692..21003
                     /locus_tag="IW715_000220"
     CDS             20692..21003
                     /locus_tag="IW715_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020851.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21118..23454)
                     /locus_tag="IW715_000221"
     CDS             complement(21118..23454)
                     /locus_tag="IW715_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1020852.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23596..24264
                     /locus_tag="IW715_000222"
     CDS             23596..24264
                     /locus_tag="IW715_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1020853.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24267..25310
                     /locus_tag="IW715_000223"
     CDS             24267..25310
                     /locus_tag="IW715_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1020854.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25547..26428)
                     /locus_tag="IW715_000224"
     CDS             complement(25547..26428)
                     /locus_tag="IW715_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1020855.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26587..27315
                     /locus_tag="IW715_000225"
     CDS             26587..27315
                     /locus_tag="IW715_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1020856.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27471..27788
                     /locus_tag="IW715_000226"
     CDS             27471..27788
                     /locus_tag="IW715_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1020857.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27781..28389
                     /locus_tag="IW715_000227"
     CDS             27781..28389
                     /locus_tag="IW715_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1020858.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28444..29235
                     /locus_tag="IW715_000228"
     CDS             28444..29235
                     /locus_tag="IW715_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1020859.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29373..29861
                     /locus_tag="IW715_000229"
     CDS             29373..29861
                     /locus_tag="IW715_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1020860.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            29912..30784
                     /locus_tag="IW715_000230"
     CDS             29912..30784
                     /locus_tag="IW715_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1020861.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            30960..31322
                     /locus_tag="IW715_000231"
     CDS             30960..31322
                     /locus_tag="IW715_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1020862.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31331..32083
                     /locus_tag="IW715_000232"
     CDS             31331..32083
                     /locus_tag="IW715_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1020863.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32097..33899
                     /locus_tag="IW715_000233"
     CDS             32097..33899
                     /locus_tag="IW715_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1020864.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34176..35217
                     /locus_tag="IW715_000234"
                     /pseudo
     CDS             34176..35217
                     /locus_tag="IW715_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(35269..37413)
                     /locus_tag="IW715_000235"
     CDS             complement(35269..37413)
                     /locus_tag="IW715_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1020865.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37770..39143)
                     /locus_tag="IW715_000236"
     CDS             complement(37770..39143)
                     /locus_tag="IW715_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1020866.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39143..39538)
                     /gene="rbsD"
                     /locus_tag="IW715_000237"
     CDS             complement(39143..39538)
                     /gene="rbsD"
                     /locus_tag="IW715_000237"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1020867.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39566..40483)
                     /gene="rbsK"
                     /locus_tag="IW715_000238"
     CDS             complement(39566..40483)
                     /gene="rbsK"
                     /locus_tag="IW715_000238"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1020868.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40591..41604)
                     /locus_tag="IW715_000239"
     CDS             complement(40591..41604)
                     /locus_tag="IW715_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1020869.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41685..42284)
                     /locus_tag="IW715_000240"
     CDS             complement(41685..42284)
                     /locus_tag="IW715_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1020870.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            42470..43171
                     /locus_tag="IW715_000241"
     CDS             42470..43171
                     /locus_tag="IW715_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1020871.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43181..45892
                     /locus_tag="IW715_000242"
     CDS             43181..45892
                     /locus_tag="IW715_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1020872.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            46343..47971
                     /gene="treC"
                     /locus_tag="IW715_000243"
     CDS             46343..47971
                     /gene="treC"
                     /locus_tag="IW715_000243"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1020873.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(48031..49212)
                     /locus_tag="IW715_000244"
     CDS             complement(48031..49212)
                     /locus_tag="IW715_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1020874.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            49464..50987
                     /locus_tag="IW715_000245"
     CDS             49464..50987
                     /locus_tag="IW715_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1020875.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(51056..51784)
                     /locus_tag="IW715_000246"
     CDS             complement(51056..51784)
                     /locus_tag="IW715_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1020876.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(51797..53078)
                     /locus_tag="IW715_000247"
                     /pseudo
     CDS             complement(51797..53078)
                     /locus_tag="IW715_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(53219..53821)
                     /locus_tag="IW715_000248"
     CDS             complement(53219..53821)
                     /locus_tag="IW715_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1020877.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            54066..54272
                     /locus_tag="IW715_000249"
     CDS             54066..54272
                     /locus_tag="IW715_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020878.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            54584..55303
                     /locus_tag="IW715_000250"
     CDS             54584..55303
                     /locus_tag="IW715_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1020879.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(55384..56067)
                     /locus_tag="IW715_000251"
     CDS             complement(55384..56067)
                     /locus_tag="IW715_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1020880.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            56285..58471
                     /locus_tag="IW715_000252"
     CDS             56285..58471
                     /locus_tag="IW715_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1020881.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            58817..59227
                     /locus_tag="IW715_000253"
     CDS             58817..59227
                     /locus_tag="IW715_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1020882.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            59241..59597
                     /locus_tag="IW715_000254"
     CDS             59241..59597
                     /locus_tag="IW715_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1020883.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        19334 a        10638 c        12105 g        18024 t
ORIGIN      
        1 acagataaac aatgtgttag aagtagtatc ttcttttaca taaaaagctg accataggat
       61 aaatggtcag cgtatttagt gagtcagcta tttagtatgg gttttctaaa ggaacgactg
      121 tctctctttt gatcaagcga tgagggatga ccaaacgtac gccactgctc aattcttcat
      181 ggatcaattc gcgcagcttt tgcatagcga cacgtcctaa ttcggagata tcgatatcga
      241 tacttgtaag gtaagggtgt gttaatgttg agaagatcga gttgttgaag ctaatgacgg
      301 agagtgtctc tggaatgaca tagccataca tttgcgccaa ctgcatcgtg cgaagggcga
      361 agatatcgtc aatgaccacc aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg
      421 acagataatc ctccggatgc tctagtacta cactggataa cgcaggaaga ccagccatca
      481 tcattgcttt ttgataaccg aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg
      541 tcacaaacag gatattctta tgtccattct ggatcaaaaa ttctgttgct tgtttaccta
      601 gaagctggtt atcattatct acataaacga tttcgttctc atgatgataa ggctgtccga
      661 tcaaagtgaa agggatgtgg ttttggtata aataatcaat gacagtatct tgactatctg
      721 aatacactag aataaaacca tctacttgtt tttgtaaatg catacgctgg acgttctcta
      781 acagcaattc aaaattttta gcagtagcga ttgctgtagt catatcataa ttccgcgctt
      841 cttcgttgat tgcttccatg atctcaagat aaaatgggtt accgatccgc tcttttgaat
      901 caagaggggg taagattacg ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag
      961 tcatgttagg aacataacct agttcatcca taatttttcg gactttcttc tttgttgtct
     1021 cggaaatact aggatgatcg ttgatgacac gagagacggt cgaggtggca acaccggctt
     1081 ttttcgccac atcttttacg gtaatagcca tgataaaaac acctttctct tgttattgtg
     1141 cagagaaccg ataaatcgtc tgactgatcc gttcttgacc aggatggagg ttgatcgatc
     1201 caaatttggg gaaatgaacg atatcaggga tttcttgcgg ttcgattgct aatccgtagt
     1261 ttgaatgcat ctgttttcca ttgacagaga aatcagcttc gaatccagtc gtagaaaata
     1321 ataccatact tttattagaa gttgctatag tcatttggcg accagaatgc ttctcgaata
     1381 attgtaagga agttttggaa agcctaggat gatgcaaaag aaatacatca tctagtccat
     1441 ctggataaca tgcaaggatc tcttgcagtg ttggggattt tctaaagtcg attggcagtt
     1501 tttctttttt tgtccaattt ccagtaggca gtttttcttg atctaattca agcacaccat
     1561 ctaaaaagac cgttaactga tgttcataga tgtctcttgt tccatttcct gaaaggttga
     1621 agtaggcatg gttggtcgga ttgcagatgg tttcaacaag gctactagca gaggttgtca
     1681 tctctaattc gttttttgtt aaacggtaag tgatcgtagc agtgattggt cctggatagg
     1741 tagaaaattc atcttttaag taaaagacca cacctattga tgatggagtt ttgaaaactt
     1801 ctacttccca aaattggaaa gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc
     1861 agtttttctc caatctatgc tcaccccaag aagcatcccg gatccttccg gctacaggtc
     1921 cgacagttgc tccaaaaaag cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca
     1981 ataaaacatt ttctgatttt ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac
     2041 cgtaattgag aagaacgact tctaagtagt cgttcttcaa tgtgattttc tttatttcag
     2101 tctctggaac tggtacgacg gagatctctg gattatccat ggtcatgcca cttttctttc
     2161 aagtaatcga tcgtaagaaa agtcgtatca gggacaagcg ggatctcctc tccaaggtct
     2221 tcagcacgtc ccacgccaat tggttgagcg ccacttgaga ggatggcttg gataccggct
     2281 tgcgcgtctt cgatcccgat acattcttct gcttttagtc cgacagcttt tgctgcaagg
     2341 aggaagatat ctggcgcagg ttttccgttt gttgcttctg ccgggtcggc aatagcatca
     2401 aagtaaggtg taagacccat tttttcaagt aggaatggcc cgtttttact tgcagaagca
     2461 agagcaatct tgattttttc ttcttttaaa gaatccagta aaggcacgac cccggggaag
     2521 acatcttttg gttcgatcgt ctggatcatc tctaaataat aatcgttttt tcttttcgct
     2581 aattctgcaa attcttcctt tgaaaaagag tgttcttttc cgccataagc taaaagcaaa
     2641 gagagtgagt cttctcggct gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta
     2701 atgccgattt cctgaccaag ctttcgccaa gcacgataat ggaattcagc agtatcggta
     2761 atgacaccgt ctaaatcaaa caatacccct ttaaacattt gcttcctcct ttcttagcgt
     2821 tatttctaga cgatccgcta aagaataaga ctgttcatag acgtaaattt ctagaggaga
     2881 accttctaat aactcgaaag taacttgttc atctgtgact gagatgaaca ataaacgtcc
     2941 gcgataattg atatggaaag aataagcttt ccaagttttt ggcagaaatg gtgcgaatct
     3001 caactgatca tgggcagttt tcatttgagc aaatccttgg acaatcgaca accagctacc
     3061 agtcatggaa gtgatgtgca agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa
     3121 gcgagctgtt cgctggtaca tttctacagc tttgctttcc attcctaatt ctgctgctaa
     3181 aatcgaatgg atacttggcg aaagggaaga ctcatggaca gtcattggtt catagaaatc
     3241 aaagtttttt tgcttttctt cttttgtaaa agtatcattg aagaaataaa tcccctgtag
     3301 tacatctgcc tgcttgatga agcaagatct aaggattttg tcccaagacc aatgctgatt
     3361 cagaggaaga tcgcttgtgg gaatatcgga tacaggcatg agatctttat ctaagaatgt
     3421 gtcatgctgg acaaagattc ctaattcttc gtcaactggg aagtacatgt tctctgcgat
     3481 atctttccat ttggctagtt cttcttcgct gatagtgata gcagcttctt tttgataagt
     3541 taaataattt tctcgcgtgt aacgcaagac ccaagctgcg atcgtatttg tataccaatt
     3601 gttattgata ttgttctcat attcgtttgg gccggtcaca ccgtggatca tgtatttatt
     3661 tgctcgtttt gaaaaatgga cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc
     3721 cagtccttca tttttcaaat attccgtatc gccggtgtaa ttcgtataat tgtagattgc
     3781 ataggcaatc gcgccatttc ggtgaatctc ctcgaatgtg atttcccact cgttatggca
     3841 ttcgacacca gtgaaggtga ccataggata aagcgcgcct tttaatcctt gttgctgagc
     3901 gttgtggatt gcttgcggca gttgattatg ccgatatttc agcagattct tagttacttc
     3961 aggtttggct aaagcaagat aaagagggac agcatacgct tctgtatccc agtaagtggc
     4021 tccgccgtat ttttctcctg tgaatccttt ggggccgata ttcaatcgtt catcttcgcc
     4081 gtagtaagta gagaatagtt ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc
     4141 tccttcgatg accacatcgg caagcttcca tcgttctgcc caagccgctg tttgctcttc
     4201 ttttgcttgg atataattgt ttgtctggtt cgttaattct gagacagctt tttctgcttg
     4261 ctgcgcttct ggaatatctc tactagtaag aacgataatt tctttttcca ctgaccatgt
     4321 ctcaccttga gataattctg cagacaagca accatgtaat gctaaagtat cttcgtgata
     4381 gtcagggatg actgattttc cattaacgaa atggcgcatt gctgcagtaa cagtaaattg
     4441 ttcaatccca aagttgtttg gaatagtttt agtcgtcaaa tagccgattt cttctatagt
     4501 tccacgatca atctcattcc aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt
     4561 tccatccaat tttgatacga gtttgactgt tccgctacct tcaagcattt ctatagttaa
     4621 atagatataa gctgattctt ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac
     4681 tttattagta gaagtagtga ttgtgaatgt acgagataat acaccatttt tcatatctaa
     4741 ttcccagtaa aagtcttctg gttccaaaga agccaagtcg atcagctgat cgttgatcga
     4801 aatatctatc gcgataaagt tcaaggcgtt gatcactttt ccaaagtatt ctggataacc
     4861 atttttccac cagccgacac gtgtcttatc gggataccag acacccgcca aataagtacc
     4921 ttgatggtgg tcccctgagt aagtttcttc aaagttcccg cgcatcccca tataaccgtt
     4981 tccaatactt gttaaagatt cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct
     5041 gattttccat ggatcaatag caaatagtcg tttgatctgt ttcatttttc tcctcctaat
     5101 gagctgtttt atacgttcat aataaacgca accgattgca caagtcaata gacaaaagaa
     5161 aatatttcat agaaaaaagc ttaaaaaaat aaatttttag gagaaaatta tagaagggaa
     5221 cacacgtatt ataggtttta tagaatggta tagacagaaa aataaaaaat aaagcattta
     5281 caaaaaagaa aacgcacgct ataatacgtt taacgaaacc gattgcgtaa aggagctaaa
     5341 cggaatgaaa aagatattta gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt
     5401 tgtggtagca gttatgccgg cagctgggtt gatgatcagt atagggaaat caattccctt
     5461 gatcaatcct gattggacgc cgcttgtcac aacgggcgga gtaattgaaa atattggttg
     5521 ggcaattatt ggtaatcttc atattttatt tgctttagca ataggaggaa gttgggcaaa
     5581 agaaagagca ggcggagcat ttgcagctgg gatctcattt attttaatca atcggatcac
     5641 tggggctatc tttggtgtca caagtgacat gcttgcgaat gaagatgcat ttacccacac
     5701 tctgttcggt acgaagatca tggttaaagg attttttaca agtgtattgg aagcaccagc
     5761 tttgaatatg ggagtattcg tagggatcat cgctggattt gtcggggcaa tggcttataa
     5821 taagtactat aattatagaa aattgccgga tgctttgtca tttttcaatg gaaagcgctt
     5881 tgttccgttt gttgttatct tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg
     5941 gccaaacgta caggctggaa tcaataattt tggtttatgg attgcagaat cacaagaaag
     6001 cgcaccgatt ctagcaccgt ttttatttgg aacattagaa cgtttactat tgccatttgg
     6061 attgcatcat atgctgacga ttcctatcaa ctatacacag cttggcggat cgtatgagat
     6121 tttatcaggc gcacaggcgg ggacacaagt atttgggcaa gatccgttat ggttggcttg
     6181 ggccactgat ttagtgaatc ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt
     6241 gcataactgg acgccggctc gtttcaaagt aggacaaatg attggttctt caggtatctt
     6301 gatgggaatg gcttttgcga tgtatcgtaa tgtcgatcct gataagaaag caagatataa
     6361 atcaatgtat ttttcagcag cgttggctgt ctttctaaca ggtgtaacag aaccattgga
     6421 gttcatgttt atgtttgctg ctgtgccatt atatgtcatt tattcagtga tccaaggggc
     6481 tgcatttgcg atggcagata tcttgccatt gcgggtccat tcatttggga atatcgaatt
     6541 gctgactcgg acaccgttag ctatcaaagc gggtctaggt ggagatctta tcaacttcgt
     6601 gttgtgtgtg atcgcttttg gtgttgttac gtatttctta gcaaatttct tgatcaaaaa
     6661 attcaacttt gctacaccgg gacgtaacgg aaattacgac aatgacagtg aagaaaccgt
     6721 tgtatctaac agcggaacag gaacagcaga tcagcaagtg gtccagatca ttcatttgct
     6781 gggaggaaaa gaaaatatca aagatgtcga tgcttgtatg actcggttac gcgtaagtgt
     6841 aaatgatcga gaaaaagtag ggactgaaga agcctggaaa cgagcagggg cgatgggatt
     6901 gatcgtcaaa gacaatggag tccaagcagt ttatggacca aaagcagatg tcttgaaatc
     6961 agatatcgaa gatctgctgc aatcaggagc agagattcca gagccagaaa tggtttctac
     7021 aggaaatgga caagcaggta gtaagcaata cttaggtata gaacaagaac tagtttccgc
     7081 agcttctgga gaagtgattc ctttatctga agtaaaagat ccggtattct cacaaaaaat
     7141 gatgggtgac ggctttgctg ttattccaac atcaagagaa gttgttgcgc cgatcgctgg
     7201 aaaagtgaca agtattttcc ctagcaaaca tgcaattggg ctggaaacga aagatggtat
     7261 cgaagttttg atccatatgg gaatcgatac agtccaaatg aatcagccag cttttgagat
     7321 tgctgtcaaa gaaggccaag aagtaggaac aggaacgaaa ttagctgaga tggatcttga
     7381 tgtgatccaa aacgaagaaa aagatccaac gatcatgatc gtatttacaa acgataaagt
     7441 agaagaagta gtgatcaagc aattaggtac agtgacagct ggtaaagtga tcggctccat
     7501 taaaatgtaa ggagaggttg tgaaaaagct gtcctgcatc aagtcataag aacagccaaa
     7561 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgagaag cagcagtttg
     7621 aatactgttt agtggagagg tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc
     7681 aaacaaaaaa tagctcctgg tccattgagc ttattactga ggagttagca ggagaacact
     7741 atttgctgga gaggttgtga caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac
     7801 aaggagaaaa ccaatgaaat tcatgacact caacacgcat agctggcttg agccagaacc
     7861 agagaaaaaa ttgcaagaat tagcagataa gatcctgtta gaagattatg aagtgattgc
     7921 tttacaagaa atcaaccaat tactggaatc tgaagaagta gaacctgcga agctaatgaa
     7981 gttctgtccg gtaaagaatc aagtacctat ccgtaaagat aattttgcct accgtttagt
     8041 tcagctttta aaggaacacg gaaaagagta tttttggagt tgggaaatga gccacatcgg
     8101 ttatgataaa tatgaagaag gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt
     8161 ccttaccgtc tcccaatttc aaaagaaaga agattatcag acacgcaaga tcttgattgg
     8221 gaaaacaaag atagatgatc aggacatttt tgtctcgagc tgccactttt cttggtggac
     8281 agataaaaaa agtggctttt attttgagtg gaaaaacttg gaaaactact ttttggaaac
     8341 tcgcgtacca ctttttttcc taggtgattt caataatcca gtagactctc aaggttatta
     8401 tactgtaaga gaaagttgcc ttcttcttca agattcgtat gttgtcgcga atgaaaaagg
     8461 aaaagcagct actgtggaaa agaaaataga tggttgggaa caaaatacag aaaaattgcg
     8521 tatagatttt attttcgtcc ctgaaggaat gcaggttaaa aaatatcaac ggatttttga
     8581 tgggatcgat agtccaatca tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa
     8641 cgaataaaaa atataacata taacataaaa aaactgatta aaataaaact ctccaaacga
     8701 ttataccgtt tggagagttt tttgatcgga tgtatttatc ccttttgcct aagcacgtaa
     8761 aagcttgtat ctatgataaa taaataaaca tagcaaggta aaccagccaa tcaaagtaag
     8821 agaaagaatg aaatagaaat agattggcgt ttgataatga agcgtgagct cgttgattcc
     8881 tttttgctgc ttcacgattg gggtcccgat atctgttact tcataatcag tcaatgtttt
     8941 tccattttgc tgtagcacag tccgatcgta gacgataaca gggatatgaa cagttttgtc
     9001 cccagtgttt ttccacttga ttgtcaaaag accgcctgga tgtgttttta taaaagttgt
     9061 ttcgttttct agtatttctt cgttataccg gtcgtattta ttgtcttttg tctccttata
     9121 gattggcaga tagtcaggtg tggttttttg gaaaacgaac aagctggtag acatatcttt
     9181 gctgaagaat gttttccttg cttcttccgg cgtatcgaat agataagtat gtcgactgac
     9241 aaatgtttca ttttcccact tttccagatg ctggtataag ttctggatcg tctgtgctga
     9301 aaaaataaga atgccaaata tacttaaaag acggaaccac ttttgttcag accactggtt
     9361 cagtgaaagg gcaaggtaaa gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg
     9421 aaactggatc aaactaataa aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa
     9481 gctagtagaa agaaaaaaga aaacaagact tgtgtacaac acgatttttt ggaataaggt
     9541 gttcttttgt tgaaagaaaa taaaaagcaa aaaacctgat aatatcaata taggaaaggc
     9601 cttagggtaa tggagccaat aactgccatc cagatctatc gccttttgac tcatttctct
     9661 gttaacaaag ggaggaagaa tcttattccc actgtaaaca atagctaatc ctgcccaaat
     9721 atttgctgtc agaacaaaat aaagcaagac cgacttaatc agttgaagca gtgcttttcc
     9781 tttttcagtc tggttaaaaa agtaataaag ataaaaaggg atatacatca aagctaacat
     9841 gatcgaagac agtaaatgga tctgtgtcat aagtcccact gagacagcga ctttgataac
     9901 agggaacttt ttatgaagga cgaaatcagc aatagggatc atacataaag gataaaaaca
     9961 agtcccccag ctagtgaatc cttggttgat agaccagtat tgaatagaat acgtactcat
    10021 gaataaaacg gctactggta cacttaccca atttcgaaca ttgacatatc tcagtaaaat
    10081 cagcatagaa aatcctgaaa taagataaag aatgaaattt gaaagaatct gataatgaaa
    10141 ccagctacct gcaagcaaca cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac
    10201 ggcattcacg atcctgccac tttgctggaa tccataaagg gaaggaaaat aatggaaatc
    10261 cccatattta atttgttgcg cggcatcata gaaccgattg taatggaatg gggcatcagc
    10321 acccaaaatt agtttatgtg tgaataaatg aggcatgaga aatgccaaag caatgaaaaa
    10381 aattgctaaa aatgggccat attttttcaa ttgtcgaact cctttgcaaa ctttagataa
    10441 attcatcata tatcctatgc gagaggtagg caagaaatta gaaaattcat gaaaatattt
    10501 atgaatttta caagaaaaac ataagtgaaa gttctaagga gagaggatgc gacataagtt
    10561 ttgttgcatc tccttgtttt gaaacagaat ttttcttaat actcgaaaca aagtagcttt
    10621 ttcatgacct ctcggtaaac ggtgttcaat cagctgcttc tcggtaataa gctcactagc
    10681 ctagaaaata tgagaagcta ttttctgact agcgctctta ttactcaaag cataacgcag
    10741 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    10801 cccaacaaaa aatatgagta gctatttttt tgggctgtac ttattacttg atgcaggaca
    10861 gctttttcac aacctcatcg gctttcattt tctatttttt tcctgaaccc atataataaa
    10921 agaaagaaca aaaaaacttg caagggagga cacatgtgaa gcagagtaag caaaaacagt
    10981 ctcgtcgact tttgtttcgt ttgtttttat taatatgtat cgtcgtaggg ttactgatcg
    11041 ctttatatcc attttatgtg gattctttaa atagttttat tgatcaaaaa cgtatcgaac
    11101 aagtacaaaa acggaccgca gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa
    11161 accaacgatt aactgaccaa ggattcaatc ctggtactga tcctttcaat gaaaagaaca
    11221 gaaatgaaag tacaacatcc agccaattag aggaatggct gatcggaagt atcaatatac
    11281 ctaagatcca attaaatatt tcactttatg atcggctcaa tggtaggatc cttgaaaatg
    11341 gagcaggcgt cttgcaagga acctctttcc cgcttggtgg aaattcgacg cattcagtga
    11401 tctctgctca cagcggttta cctaatcgac ggttatttac cgaattagat cgtttagaaa
    11461 acggagacac gtttattttg actgttttgg gggagaaatt ggcttatcaa gttaaaaata
    11521 tacaagtggt actgccagat gacacgtctg ttctaacgat ttcagaagga gaagacttag
    11581 ttaccttgct tacatgtacg ccgtatatga tcaacacgca tcgtttgcta gtgacaggac
    11641 atcggattcc ttataacgaa tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa
    11701 ccatgcggca atggtttatt cttgcaggaa cgataatagc tgtagtcata ctgttagtgt
    11761 ttatcggacg actaatctat cagtatagat taagtaaaaa agtgcttgat ttttcattta
    11821 ttattagtga ttcgtcaggg aaaccggtaa atggtagttc attcattttg aaacataaga
    11881 aaaaaacact tactagaaat ggtgtgccgt tttcagtaca aagcgaccat catgggaaag
    11941 tcaagctgga tcaattgcct ggtggaactt atcgaatcgt ttcagctgat gatcctaaag
    12001 ttgctgtttc ttttggtatt agaaaagtaa aacaagagaa gatatacttt tttgaggggc
    12061 gaaaacttgt aaaagagctt caaaaaaatg gtttttattt taaaatcaat gaataagtaa
    12121 ggagagaaaa aatgatccgc acaacaacaa aattgtctgc caaagaactg gaatctgttc
    12181 tatcgatttg gctgcatgct aacttggatt ctcatgaatt cattccatca acttattggg
    12241 agaatcaagt agaatctgtt aggaaagctt taccagatgc agtgatttac ttagcagaaa
    12301 tagatcaaga aattattggt tttgctgggg tgattgatca gtttatcgca ggaatctttg
    12361 tgaaaaggga ataccggcaa caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa
    12421 ccaataaaac actgactttg acagtctatc aaaaaaatca atcagctatt catttctatc
    12481 tttcacaagg ttttacaatt caaacacagc aaaaagatga agcagtaaat gaggaggaac
    12541 ttgtgatgaa atggaatcaa aacgctgatt tttgatgaac agaataacga aataatgctc
    12601 ccagcctaat tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg
    12661 aatatgcagt tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag
    12721 ctcaaaaaac ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc
    12781 aaaacgctga tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata
    12841 agcccactag cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa
    12901 gcataacgct gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat
    12961 tagctcaaaa aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag
    13021 gtcagaacgc tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt
    13081 catcgaagta ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa
    13141 taacatcttc attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa
    13201 caagtaattt tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt
    13261 cgttttggaa agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca
    13321 ttccgtgaag tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct
    13381 ctgtccaagt gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt
    13441 ttacccaacg gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt
    13501 tggcatacca ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa
    13561 aatcttccaa ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt
    13621 aataaactac atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag
    13681 gagggaaaac tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt
    13741 aagaagaaga tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt
    13801 tggcaagcaa aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc
    13861 attcagcatc aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca
    13921 gaagaaggga aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt
    13981 aatcgagacg aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta
    14041 gtagaaatta ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag
    14101 cgaaaaaaaa cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg acactacctg
    14161 attttagcgc atgcgatcaa tcgggaaaca gaatatgcaa attggctaac agatgtatta
    14221 gaagaaagta accgaaaaaa ataaaaaaat atccgaacag tgatggagaa ttcctttcta
    14281 tatgaaagaa acgcagatca ccgttcggat attcttatgt tcaaacaggc cttttttcaa
    14341 gtatttatgt agcatctact tattaaaaag cttggtactt aggagattag tggcgtttag
    14401 agttctttta acaactgttc gtagtcagct ttttcatcga caggaaggag cttgtaagtt
    14461 aattcgtctg tctcgttgtc atgaatgatg tacatcttag cattctcttc tttgaagtta
    14521 ccgatttgta aaatcgtata gcgttcgtcg atcaattcga tatctttgat tgcttttgaa
    14581 aaaactttac ggacatggtg cattttcttt tggcggattt ctgccttttc tgcagctttt
    14641 gacaaagtaa atccgtcatc taaatatttt ttgatcagtt ctacttttat gactgtaggt
    14701 aaacgatatt ttctaggaca attcgcatca gcttcgatcg aacgaatgaa tcccttttgt
    14761 tcccaatacc ttaattgacg tggagacaca cctacgatat cacttaattc actgatgcca
    14821 acaaccaatt gatcattttc caacaaacgt ttaagggaag ccccaaccat ttttgtcact
    14881 ccttttgtcc tcttgcttcc cttaattata tccgtattaa cttatgtgtc aagtaggttg
    14941 acaaatagcg aatttaggac taaagtattc tagtagtttc agaaaaaatt cagaaagaag
    15001 gagaaatatg agtaacaacc aacccgtaga tatttacgga aaaccatata atcggacact
    15061 tttagttgtt gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac
    15121 gacagctttt cctacattga tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt
    15181 aacgactggt ttcttgctag tcaacgggat catgatccca ataacagcat ggctgatcaa
    15241 taaattcagt tcaagaaatt tatatctttc tgccatgacg atctttttga tcggaacgat
    15301 tacttgcttt acagcaccta attttagtac attactaatc ggtcgtttga tccaagcttg
    15361 cggtgtggga gtatcgatgc cattattgca aaatatcatg ctatctattt tcccgccaga
    15421 aaaaagagga tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc ctgctttagg
    15481 ccctacatta tctggttata tcattgataa ctaccattgg cgtgacttgt ttggtatggt
    15541 actgccaatc gttgttcttg tcttggtttt agcattcttc ctaatgaaaa gtgttattcc
    15601 attgtcaaat cctagcattg atattttatc cgctatttta tcaacgattg gattcggtag
    15661 tttactttac ggattttcta gtgtaggaga tgacggctgg ggaagcttga aagtaattgg
    15721 tttcttagtt gttggtgtga ttttcatcgc cttgttctca tggcgtcaat tacatttgga
    15781 acatccattc ttggaacttc gtgtattcaa atcatctaca ttcacgattg cagctgtttt
    15841 agctggtgta acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat tgtatattca
    15901 aaatattcgt ggagaatcag cattccattc tggcttgatg ttgttgccgg gtgcattgat
    15961 catgggggct atgatgcctg tgacgggtgc gattttcgat aaatacggcg cgaaacgttt
    16021 agccatcaca gggatgttca ttttgacagc ggcaactgca ccatttgctt tcttgacaaa
    16081 aacgactcca gttttatata tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat
    16141 ggtcatgatg ccggtaacga cttctggtat gaatgcattg ccaaataact tgatcagtca
    16201 tgggacagcg gtaaataata cattccgtca aatcgcaagc tcgattggta cagctatttt
    16261 gatcagtgtg ttgacgaatg tgacaaaagc aaatatccca agcaaatctg tattggataa
    16321 tacgccatta gcttataaag atcaggcaat caatgccaca ctgtcaggtt accatgccgc
    16381 gtttatcgta gcgatcatct ttgggattct tggctttatt attgcctttt tattgaagaa
    16441 aaaagaacct gttctaatga aagcggggga taatgcatga ttattggagt tttgattgtc
    16501 agcttgcttg cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca aaccttttta
    16561 tctttgattt tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc
    16621 aatcactttg gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt ttccagtgct
    16681 gattcccaag gagcagatat gcttttgtat aaagctttag ggaatggaaa agaaaaagtg
    16741 tatctttacc gaacaaatga aaaacaagaa aaaccaaaag caactggtac ggataacgaa
    16801 acaaataaag tagaaaagac agacggcgat gctgaaaaag tgacgaaaac tacttattgg
    16861 gaatacaaaa acgacatgta taaattctgg ttcaatattg ctgataacaa tcacgaatat
    16921 gacagccgag tgaatacatt caaaatccca gagacatggg tagaattaag tacggaccaa
    16981 gcagccaagt tagctgaatt agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa
    17041 gcttatgttc aagacggtat ggtaaaagca atgactgaaa atccaaaaat gagcaaagca
    17101 gagcaagaac aacgtacgaa agccttagct gctgaattcc aacaacaagc attcgcaaaa
    17161 ttagtgaaag aagcaaaagg cgaataatat tttgaacacc gtttaaggaa gaggttgtga
    17221 gcctgccgtt taactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttgt
    17281 tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg
    17341 aactgcgaca agacttttgt cacagttcct tctttttttt gctatgctat actagatgta
    17401 aaaaatagga gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc
    17461 gaagtcggca agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt
    17521 gctggaagag ttaaagtcca agcaactttt gacaatgtgc catatgaagg cagtatcgtg
    17581 aatatgggcg tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat
    17641 attcgtaaaa acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa
    17701 taaaagaggt tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca
    17761 gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt
    17821 tcttactact cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag
    17881 ctgtccctcg gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt
    17941 ctctcatact caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac
    18001 catctttata ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa
    18061 tcttgtaacc taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat
    18121 ggtcaacatg gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca
    18181 cagctatttt ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac
    18241 gtacagtttg catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat
    18301 ctccgcctgt taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg
    18361 tgaaacctag tttggttact tcaactatcg tttctttttc catattaatc accctgccaa
    18421 cgttttgaat gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta
    18481 acaagatctt ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc
    18541 acacctagtc gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca
    18601 cgaggaacaa tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc
    18661 aggttgtcgc ctaaaccaac agagatacct gcgactgttt tcatacttgc cggtacgatg
    18721 atcattccat cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa
    18781 taatggtaat caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag
    18841 ttctgttttg cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt
    18901 ttcttcacta ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt
    18961 tttttcttcc gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca
    19021 gttgcagatc attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc
    19081 acgtacaaaa tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact
    19141 tatcccacgg aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg
    19201 atggttcctc attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa
    19261 agtccaaact acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt
    19321 cttcaattct ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc
    19381 cgcttcgttt tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg
    19441 attgtagaca tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc
    19501 ttctgtagga ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat
    19561 gattggattg tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt
    19621 agcatcccca gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc
    19681 ttcttgcatt gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat
    19741 acccacttca tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc
    19801 tgcacaccat agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat
    19861 ggcaattgct ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc
    19921 gaattgtttt tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt
    19981 atctttatct tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc
    20041 tgatcctaag actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg
    20101 tgcaggcaaa atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg
    20161 tgctggagcg tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt
    20221 caagaaacga ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact
    20281 agccattgtt ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat
    20341 agttggtcct tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac
    20401 accagataat tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag
    20461 tgtttttagt tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc
    20521 ctccttagtt gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact
    20581 tgttcggtaa agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat
    20641 gatatactta tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa
    20701 atggaggagt catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt
    20761 acatttgaaa acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat
    20821 gccttgcaat ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt
    20881 aatatgtgga ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga
    20941 aaagacaggt atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta
    21001 taaaaaataa ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc
    21061 tgttttctac tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta
    21121 ttctgcacgc aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc
    21181 gccaagcatc gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag
    21241 ttgagcaaca ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc
    21301 tagccatgca ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc
    21361 gtcaaatacg cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc
    21421 ttttgtccgt tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac
    21481 taaagagatc cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt
    21541 cagttcagtc atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc
    21601 tttattgtaa tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata
    21661 aatcatgata cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc
    21721 attttcgtca actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac
    21781 gatttctgat tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat
    21841 tcctggagat aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt
    21901 caattcatca gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc
    21961 agttggaagt tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat
    22021 atcatcaaaa tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt
    22081 gatattagta ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata
    22141 acttccggca agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg
    22201 tgtagggaaa cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag
    22261 agattccgta tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc
    22321 acgtaaaaga ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc
    22381 gatgtctgtt acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc
    22441 ttcttcgctg acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc
    22501 caatccggca tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt
    22561 cgccatcgtt tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag
    22621 aacgatcgcg attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc
    22681 tttttttgtt cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt
    22741 tctgcgtaag ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa
    22801 gatcttacca tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt
    22861 atgtgtgacc atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg
    22921 gatacttgtt tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc
    22981 attggctagt gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg
    23041 ctttttatgc atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg
    23101 tttttcgtct ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc
    23161 tagatgacca atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc
    23221 atcccagtca ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga
    23281 atcataatta tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg
    23341 acctaaaatc gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt
    23401 ggtggtggtt tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac
    23461 ccttctcccg tattgaaata ttcggtatac taataagtat atcagaacaa tgatcgtagt
    23521 atacgaaata aatgttaact gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa
    23581 aacagaggga gatcaatgaa tcgtatttta gtagtagaag atgatccgca attacagttg
    23641 ctttaccgtt ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt gaatggagag
    23701 gatgcgctga aaaaactaga agcgacacgt atagaattaa tcattacaga tatcatgatg
    23761 ccgaaaatgg atggctatga cttgctagac tcattacgta gcaccagatt tgacacacct
    23821 gttctaatga ttacggcaaa ggccgatttt gaggataaga aacgcgggtt caaattagga
    23881 gcagatgatt atatgacaaa acccatcgat gtcaatgaga tgatcctacg tgtggaagca
    23941 ttgcttcgac gttcaaagat caattacagc agtagcttga taatcggaca aacaaaatta
    24001 gagctagatg cttatacagt gttacaagat cagcattcgt tgattctgcc tcaaaaagaa
    24061 tttctgcttc tgtataaatt actgtcttat cccaacaaga tcttcactcg ccaacagttg
    24121 atggatgata tttggggatt agatacgaat acagaagaac gaacgatcga tgtacatatc
    24181 aaacgtttaa ggacccgttt tgaaaagacg gaagattttc aaatcatcac agtacgggga
    24241 ctaggctata aggcggttat ttaaagatga aaaaaaagct attttcacaa ttatggatct
    24301 attttgctag tattgtcttc atttcgatct tagtgacact gctgtgtttt ctcggtttta
    24361 ttttcttatt gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat
    24421 acccgttatt cgtctttgcg gggttcagta tggtcgtggg tacaggaata tcgatttttg
    24481 ttgggcggcg tatcctgcat ccaatcagtg cgctagggac aaatatgagt ctggtagcaa
    24541 cgggagattt ttcgattcga atggatgaac agcagaaagt agcagaagta cagcaactgt
    24601 ataaagattt caacgttatg gtccaagaac tcaatagtat cgagacactg aggaatgact
    24661 ttgtgtccag tgtatcccat gaattcaaaa caccactagc aacaatccaa ggatatgttc
    24721 aattgttaca agccccaaat ctctctgacg aagaacgcca aatattttta tatcggatta
    24781 tcgaaagtat cacgcagctt tcacaattaa cagagaacat cttaaaattg aacaaactgg
    24841 aaaaccagcg tatccaactg gaaaagaaag agtatcgatt agatgaacaa atcagagaag
    24901 taatcgtttt ttttcagcct aaatgggaga aagaacagct agaattggat atcgaacttg
    24961 ctgcagtaaa ttacacaggc aatgaagagt ttttgtacca agtgtggctg aatatcatgg
    25021 ataacgccat aaagtacaat caaatcaatg gacagattca tatcaaacta tttgagacag
    25081 ccacagagat tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa gagaccagag
    25141 atcgaatgtt tgaaaaattt tatcaaggag atacaagtcg tcaaatttct gggaatgggc
    25201 taggactttc actggttaaa aaaattctag aactgcatga tggacgtatt gattatagca
    25261 gtatcgaagg agtaggaacg acagcaatga tacggttaag taaacaatag gaaaaagaat
    25321 aaaaccccta aaaattctat tgaaggtttt taggggtttt tagtcgtttt tttgtgatta
    25381 gggaaatgtt cttacggttc ttttggcata aaaatcgctg ctttcttatt gaactacgag
    25441 aaatgttaga ggtgtcattg aaacaaggac atgatgcaag aaagagagcg aaagtcatgc
    25501 gtcgctctct ttttttgggt ggatagatgg acagaaaatg tagggcttag ttttcttctt
    25561 gatttaaata ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa
    25621 atgcaagata actctctgtt gttggagaaa tagggagaat cacagtattt tcttttaata
    25681 gattcttgac tgttttttcc ataacaatag caatccccat gtcatttgca atcatattga
    25741 tgatcaagtc gattcttgct cctttataag agatttttgg ttcaaatcca gcttcgcgac
    25801 acagatctag tacaggttga tacaagttcg tagtggggcc aaggagcaaa aagttctcct
    25861 ttttcagttc gcttagatcc aatttctttt tactagctaa aggatgattt ttaggtagaa
    25921 ctgctacaaa atcgtctgtt tcagtaggca accactcaat cgaacttggt ggggcctgga
    25981 aacttctagc aaagtggatc gtgtcactat gttcattggc gtcattgtaa gttaattcga
    26041 cactttccac ttctttcaac tggactgtaa attcaggatg gcgtttcaaa aactcggtga
    26101 ttttggaaaa acctttataa ttgatcattg tcggtatcgt taagatattt aatgaaagat
    26161 tgttgtcttt cgtataggtt tcaagcactt cttgcatagc atggtattgt tctacgacat
    26221 tttttacata tgggagcaat aagcttccag cttcggtcaa ttcgattttt ctattggcgc
    26281 gttcgaataa tttgacgtct aattctgttt ctaacgaaag aatctgttta gaaatatttc
    26341 cttgggtggt gaattgtcga tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa
    26401 gaaaaacttc gaatttatgg atatccatca tattcctcct acttattcca ataaagaata
    26461 ctttaacaca aaaaatgaat tggtgtaatc ttctctgtct gttacaatct ttcttgcagc
    26521 gaaagcaata attacagaaa tttttctgat ttttttcgct tcgtagaaaa agagaggagt
    26581 tttttgatgc caactggagt tttgatcaat gtaggttccg ttttgtttgg cggactaatt
    26641 ggcggattag taggaaataa attaagtgaa cattttaaag cacagctgac gatggttttt
    26701 ggtgtgtgtt cgatggggat gggaatctat tccattgcgc cgatgaaaaa tatgccggct
    26761 gttatttttg ctttagtgat aggaacagcg attggtttga ttgttcattt aggtaatggg
    26821 ataaataagg gggctgcgct gatgcaagta cctatttcta aaatcttccc tagtgaaaag
    26881 cttggtatga cccacgatga atttatcagc acattagtga cggtcattgt tctattttgt
    26941 gcaagtggta cggggatcta tggaagttta gattctggga tgacaggaga cagtacgatt
    27001 ttgatttcta aatctgtcct tgattttttc acagcagcta tttttgcgtg taatttaggg
    27061 tatgtcgttt ctgtgatagc aattccccaa ttcattattt tttatatttt gttcttattg
    27121 gcaaaattta tttatccatt gactacacca gacatgatct tagattttaa agcttgcggc
    27181 ggattcttga tggtcgctac cggttttaga atgatcaacg tgaaaatgtt cccagtagct
    27241 gacatgatac ctgcaatgat cgttatcatg ccactgagct ggatgtggac aaattggatt
    27301 atgccactgt tataataaaa atcaaagaga agccattttt tgctgactaa aaaggatcaa
    27361 atcttttagt cagttttttt gtatttgaca aactgatttt gaaactatat tatatgttgt
    27421 ataatataga ttgaaataaa atattacact tgagaataga aggtgacgta atgcacatcc
    27481 agattccaac tgttcttctt gatggtactg tgctagcagc attaagcaag gaagatatgt
    27541 atgggtatgc cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa tctacactat
    27601 atccggtttt gagaagattg aaaaaagaag gatatcttga gacctatgat gaaccttttg
    27661 aaggcagaaa ccgtcgatat tactcgttga caccaatggg aagtcgtcat ttagaagaaa
    27721 ttattgatga atggattgtt tttcgagatt ctttagaccg gttattggag gagagcacaa
    27781 atgaatgaat atttgaatga agttgtggat catcttcaag aaattcctga agacgatcgt
    27841 tttgatttga tccagtatta tgaggaatat tttttagatt ctggaaaaac attagatcag
    27901 atcattgatg aatatggtac accaaagcag tttgccttaa aattaaaaat cagttacttt
    27961 tcagaagaag aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg
    28021 atttggttaa tcattatcgg tttatttgct tctcctgtcc tcattccact agcactgggg
    28081 tttatattgg ttattggggg actattggtt gctttactag cgctcatttt tagtatctat
    28141 gttttatgta tcagtatctt aggtgttggc ctattcatga tcatctcagg aatcagtgtg
    28201 tttggacaat cgattctgag tggattattc tttatcggtg tcggtctgtt cgccacagga
    28261 agcgcagtat ttatttcgcc actgttgctg aaaggaacaa aagggctttt ttatgtcatg
    28321 atgaactttg ttaaatggat gggacgacgt tttattacaa aaagagatct gcattcagcg
    28381 ggcgtttgaa ggaggacaca taaattgaga ttgaaacatt ttttgattac cggtattggt
    28441 ctgatgctta ctggtggcct cctagcaggt gcaacttata cattaggcgc tcaaaagagt
    28501 ctgacttggg ataatggtcc tcggttgatc gatatggtta ctgagacaaa aaaaatcaag
    28561 gatgcaaaca aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat acgcggattg
    28621 gattttgaga taaaaacgac ttatgataag acagaaaagc cagaaataaa ggagaaaaat
    28681 ggcgtgttga ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag tttagaagac
    28741 acaagtgcac aaatcactat tgttgtccct gcagatcatg aattatctga attgacaatc
    28801 gaagggaata atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag
    28861 acacatgatg gctgggtatc gttcaatgaa tttacaggag gaacaataac tgcgaaagat
    28921 gaattaggct cacttcgatt agatgaggcc aatgtggata acttgatcgt cacaggtgaa
    28981 aacagcaact tccatctaga agacagtttg atcagcaaag aaggaaaagt catcctaaac
    29041 ggtggcaatt tatctttgca tgagatggaa aacagtggct atactatcca aacgtctggt
    29101 aaatcttata ttgaaaaaga ctaccagcca ataacaaata ctttgttaga acaaggacag
    29161 aaaaagctaa agatcactgc taaagacaca tcgatcaata ttacgacgga tcaagaggac
    29221 aatgaaacag attgatcctg tactcaactg gatattttaa gaaaaaacag caaagatcct
    29281 tccaatcttt gctgtttttc atcgcttaac gcacataact atagtaaaat aagaaagaaa
    29341 atatctaaaa agaaaacagg gtgaaagagt atatgaaggt agaagaaggt caaagagaaa
    29401 agttaaaaac agaaatggaa agattacata cttatatcac tcaactaagc caaaccttct
    29461 atgatccaga caaagaaaaa gtgatggtta attacccgaa caattcagaa ggtcgccaat
    29521 tagagcaagt gtaccatgaa gttttcaaac atttattgac ggtgaagaaa gagctggatt
    29581 attattcttt accgattatc gatacaggaa tattgaagta tgatgggaag aaggaacgat
    29641 ttatctttaa atctgttcgg gaagatttgc ctctttcagc agggatggat ctagaggtct
    29701 tggtagaaga ctattttaca gaagaaaagc actgggttcg aacaaaattg gattatttac
    29761 cgcaggcagc cggagggaca caggcaagcg gttggtacat cactgaagac aaagagttag
    29821 aactagaagg tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac attagatatt
    29881 tattgaagga taaaggagcg caggaagtag catgagagaa aaaagagcat tttatatcaa
    29941 tggttatgca gggattgtcg gattggtcgt attagcttta gttggattgt ttttcttcta
    30001 tttaggtatg tggcaagcga aagtttgggc acttttttta agtatttttc tttggttgat
    30061 cacgttattg ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa aagtaatttt
    30121 gttttttgga caatatttag gtacgattcg agaaaatggc tttttcctta cgataccttt
    30181 ggcacaaaaa atgactgttt cgttgaaagt tcggaatttc aatagttctg ttttaaaagt
    30241 caatgatttg gatggaaacc ccatcgaaat ctcagcagta gtagtcttta aagtcgttga
    30301 tacagcaaag gcattattcg atgtagcgta ttatcaagat tttgtagaaa tccaaagtga
    30361 gacagccatt cggcatatcg ctagccaata cccgtatgat acattcaatg agaatgattt
    30421 gacgcttcga ggaaacacga cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg
    30481 tttagcagta gcaggtgtcg aagtaatcga aacacgcttg aaccatttag cctatgcaac
    30541 agaaatcgct agtgccatgc tccaacgtca acaagcaaaa gccattttgt ctgctcgaca
    30601 aaccattgtt gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg
    30661 acaagaaatc aatttcaccg atgatcggaa agtccaattg atcaataatt tacttgtctc
    30721 aatcattaca gacaaaggaa cgcaacctgt tatcaataca ggcgacatga aagaccgtaa
    30781 gtgacatact ttttgtaaga taggaagaag gagtttagga cggattattg tcttgaactc
    30841 tttttttagt tgttttaaag aaaatgacaa aattgaaaga agaatgatag ttgattcgaa
    30901 ggcagaattg ttcgcttttt tctataatca gtatataaag taagagagaa ggggaaaaag
    30961 tgaaaaaact tctgatcggt ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa
    31021 tcgcagatta cgtagttatg ggtggagaag aatactatgt acaaattacg acagatggta
    31081 caaaaacagt ttcaaaatca gactctggac aagaaacagt caactataga tatatcctgc
    31141 ctggctatac gaaagagggt aaggaaaaag aactggactt caagggacaa ttgactcgac
    31201 cgcttcgtaa aggagcatat ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt
    31261 atgaagaagt cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta attaaagggt
    31321 agggaaagaa atgaacaaaa tagtcgaagt acagaatgta acaaaagtat acggaaaaaa
    31381 caatgagaaa aagacgcaag cattaagcgg catcagcttc gatgtagaaa aaggcgaatt
    31441 tattgggatc atgggcgctt ctggttctgg taagtcgaca cttttgaata ttttatccac
    31501 attagataaa ccaacagatg ggcatatccg aatcaatcaa aaagatgtga ccacattaaa
    31561 aggaaatcaa ctagcagatt ttcgtgcgaa tgagattggc tttattttcc aagatttcaa
    31621 tttattagaa agcttgacag cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt
    31681 cagaccaaaa gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat ccatctctca
    31741 tattttggaa agctatcctt ctgagatttc cggtggacaa aaacaacggg tcgccgccgc
    31801 acgtgcattg atcacacaac caacgattct attaggggat gagcctacag gtgcgctgga
    31861 ttccaaaagt gcccgggatc ttttagatac gatggacgaa ttgaatacaa aagatcatgt
    31921 atcgattctt cttgtgacgc atgatccgtt ttctgcaagt tattgccaac gtattttatt
    31981 catcaaggat ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt
    32041 ctatcgagaa atactatcta ttcttggaaa cttggaacag tagggggaga ttgactatgc
    32101 tttggaaatt atcacttact gggatcaaga gccgtttaag agactacttg gtgttattct
    32161 caggattagt tatggcatct gctatttttt acatgtttga atcaatggca agcaacgaag
    32221 cttttttgaa aagtaatacg atcgttgcgg ctacagttat cattttccga ttcggttctg
    32281 ttttactagg gatcatcaca ttcgtttata ttttatatgc aaattccttt ttgatgacga
    32341 tgcggcaaaa agactatgca atgtttatga tgctgggagc aaaaggcaga aaaatcgcac
    32401 agatgatttt ttctgaaaca ttcttagtag ggatcgcagc tactttagtg ggttcagctg
    32461 tcggtgtagg attgacaagt atcgtccacc gattattagt ggatcagtta gatctcaata
    32521 tttcgcattt tacacctttt agtattcacg ggttgttgat cactgttttg ttttttgctg
    32581 ttttgttttt attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag
    32641 cattgattcg tgcggatcag acaccaacaa gaatcaagca gcataaattc ttgtttttcc
    32701 ttgaagtagt actgggaatt gcttttctag gctgcggtta ctatatgatg aaacacgtgg
    32761 ggacctatca agtctttggt ataggggtag ccttagttac gatcgtatta ggaacttatt
    32821 tcattttcca ttctgtgatc attttctttt tatcattact aaagaaatca gaacagattt
    32881 cattgaaaaa attgaacaat ttcacattgt cacagttaaa ctttcgtatt cgtgaatata
    32941 cacaaatgct ctcaatggtc gctatgctgt ttgccttggc actgggcgct ttgacggtag
    33001 gattaggttt taaaaatgaa attgcagact tgacagaaaa caattctgga tatgatctag
    33061 tgatgaataa tcctcgggca gaagatcagc aaaaaatcaa agaactttca ccaacacttt
    33121 ctgcttctta tactcaaaaa gaagatgcgg gaacgattta ctataatgca agtgaatttg
    33181 cggctcaacc attgatcatg ataaaacatg aagatggaac accgccgaag atcacgaaag
    33241 aaaaagttcc tgctaaaaaa atgaatgaac tgactgtaca agaggaactt cgaagctatg
    33301 aattgccgga acagcaagaa aaagaaatca aggtagtcag tcaatcagaa tttgatcaac
    33361 tgaacttgcc acaatccact ttacagctag ttcaggtaaa agacttccaa gcaaacctga
    33421 aaccaatcga aacacttgtt gctcaaaata aaatgaacaa tccatctttg caaaaagtag
    33481 agtacagcaa ccagaaatat gaaatgtatg aagtgtataa tggtctattc tctggttttg
    33541 aatttatggg attcttccta ggtttagctt tcttgaccat gcttgctagc tgtttgatgt
    33601 tcaagatctt gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag
    33661 gaacacgacg cgggttgctt aaacaatcga tccgtagaga agtaggcgtg ttattcttag
    33721 caccaggaat cttaggtgta gtccatgtct tattcggatt acaaatgttt ggtctattca
    33781 tgcaaaatcc atacaaagat atttgggttc cgtttacgat attcttcgta ttgtatttca
    33841 tctactatgt actgacgaca tggttatata ctggaatcgt attgaaaaag agattgtgaa
    33901 aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa aatagctcct catatttttc
    33961 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga
    34021 ggttgtgaca atatttttgt cacaaccttt ttttcacggt cttttattag aatatagtaa
    34081 attttttctc gttactcacg gaataagctg ttaagaaaac ggtttccatg tatgctggaa
    34141 tcagtgcaaa tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa caaaattttg
    34201 gcgaaaacta tcacttgatt acgatcacga atcaaaatgg gacatctttg tctgtcagtg
    34261 atcttggggc acgggttgtt cattttcaaa caataatcga taatgaaaaa cgagagttga
    34321 tcttaggttt tgatgaagca aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa
    34381 tcggacgtat ggctggacgg attacgaatg gcacattcaa attagatgat gaaacgttcg
    34441 aagtaaaaac agacccggac actggtcatt ggtttacatg gtggtgcacc tagctttgaa
    34501 gccaagcagt ggcattatac agtcatcaat ggtgaaaacg aagcatcggt cattttctat
    34561 cagactagcc cagatatgga aaacggattt cctggaactt tagatgtaga agtacggtat
    34621 acgctgacga acgatgatac atggcgagtg acggtccaag ggatcagtga ccgcaaaaca
    34681 gtattcaatc ctacaaatca tgtctatttc aatttaaccg gtgatccagc acagtcaatc
    34741 gacgaacata tactttggtt gaatagccga ttatttgcta cgctcaataa agatacaacg
    34801 cctacaggag aagtagtttc agtaagtggc acagcatttg acttccaacg tcctaaaaaa
    34861 ttaggtgagg tctttgccag tgattttcgc caatcaatga tagtgggcgg catcgatcat
    34921 ccatttttgt tgaaaaaaca atctggttta ctaaatgctg cggaattgac aagcccagat
    34981 gaaaaagtat cgatccaagt aaaaacagat gcttcaggaa tcgtgatttt tacagcaaat
    35041 tttggagaag atggaccgga aatgcgcggg aataaattag taaatcatgg cggtatcaca
    35101 tttgagactc aagaactgcc aggagcggag cgacttgaaa gttttggaga tgtcactctg
    35161 gaacctaacc aagtacgcga gtttgtaacg gaatacaaaa taaatataaa gaaatagaat
    35221 aaattaaaaa gggagtgtga cagtgtcaca tccccttttt aatttattct atttctttaa
    35281 aggcagatca agccagaatg atttgtctaa acttaacaag gttagtacag gaacagttgc
    35341 agctttaact gtacgagtag ttgtttctgg tatctgtctt gccgtaaaat tgttccacaa
    35401 atcccagatt gaagcgacac ggaagatgga agaagcgttg ttgacgcgtt ttatcgttga
    35461 aaattctaat cctgtgattg gcacgccatc tgttgtccat ttgattggca catcgaatcg
    35521 cttagacata ttgaggattt cagcgtttgc ttcttcagtt gttgctgctt cttttttcgg
    35581 ttcatcatat tttgtcaggt cgtacgcttc gatgatcgcg ttcaattttt gtgaatattg
    35641 gcgatctgta gcataacgtc cttccaaata tttggttgct tctttgtagt cttttgtttc
    35701 cgacttccaa gttggtttgt agaaatcagg attgccagtc agtccatttt tgatcaagtc
    35761 ggcatagtct tccaaacttt ccttgtaaga aggatattta cggaattgtg cgcggatttg
    35821 gtagctttgt ccggaactat cttgttcaag tgtattgaag gtcacgctgc ttcctttata
    35881 tgatcctttg ataccaaaaa ggttatgatt tggttcgctt gataataagc tgtttccaga
    35941 tccactttca aggatagctt gagccatcat gactgatgcg tagattcctt ctttttgccc
    36001 gatttcctgt gcatcatctg caatcttttt caagaactga tcagttgttt ggttttgact
    36061 gaaatggaac gagccatcta atccatctaa aatagatgag ccgcctgtca gcgaacctga
    36121 agcagcaggt aaacctgcta agtttactcg gacagcaaaa tcaggcgtga aatctgaaat
    36181 agaagttact cgaactttat ctcctggttg aggcgcatgg ataaattgtc cgttcccaat
    36241 atagatacct acatggtgag taacaccgga agaaggtccc cagaagacga ggtcgccagg
    36301 ttgggcctga cttactggaa tctgcgtacc tgcatattgc tgttcgcctg tccagccacc
    36361 gatataatga ccggttgctt tcatatatac gtaataagtc aaaccagaac agtcaaaagt
    36421 atttggacct tttgctcccc atacatatgg ttttccaaga tgcttgaatg cttctcgaac
    36481 gattgcttgt tggacattcg ttgtatttgt aggcaataca gcatcaattg gtgattttac
    36541 aacaaaatca gaagaagcag aagatggatt ctctataccg ctttgatttg ctgtatttgg
    36601 acgagatgaa gcattgttcg ttccgccatt cgatgaacca ttgtttggat ggtttggttc
    36661 tctctgagta tttccattgt tattcgatcc agatgggttt gtttgattcg aagaattccc
    36721 gctgttcgaa ttattgttat tattgtttga attatttgaa ccattattat tgttgttatt
    36781 acccacatct ggtttgtttg gcgtttccgg atctggtgta ctagggactt cttcactagg
    36841 atcagttggt ttctctgttg aagtatccgg tccagcttta aattctgcag taatagataa
    36901 attgttcgta cctactgtaa aggtctttgc atcataattt acttctttgg caccattaac
    36961 agcgactgaa gcaatttgtt caccttcagc tggcgtaata gtgtaagtta ccttcgtttc
    37021 cgctaataaa ccttctaccg ttccactttc cgtaatatcc aaaggttgct gttcagggga
    37081 atccgctgtc gaagcagtca gttggatctt accaatcaaa gaaggagcaa ttgtaaggga
    37141 atatgtctcg gctggctttt cattttctga tggatcagcc ggatcaattt cagttggctc
    37201 ggtcgatcct gttgttgagt tagttgttcc agttgggtct tctgtaaaag aatccgttgt
    37261 ttcactagta gctgagctgg atgtagtcgt atctgttgtg actgtttgat cagctatttg
    37321 attatctacg gcaaatacgg tattggtcga caaaacagac ggagcaatca gccaagtagc
    37381 agctagcaaa cgtatcgttt ttttattttt cataataaac tagtcctttt cctttcattg
    37441 attttctgtc ttattcagca ttcattagac attttttgga acagataatc gtgtcgaaaa
    37501 gctttttctt ttcataaatc tgtctgtgta ttctaaataa aacaatttgt aaataagcaa
    37561 cgagcattca ttcgtttttc tttgacgttc attttattgc atagaccacc tttgtttggg
    37621 gataaacaag tggggataaa aatggttatg catatgtaaa ttataccaaa gtttggaaag
    37681 tatcacaaaa atttataaaa ataatgatcc taaaaagaaa aagactgaaa tatgtgataa
    37741 acatctcata cttcagtctt tttcattaac tattcataag acattaggtc ttcatcaaac
    37801 ccgctaacga gagtagtgat cattgctacg actagactaa cagccagtcc tgctaagtac
    37861 cagaaaaaag taccggaaat gaatgcaggc aatgtggtca gactaccaaa tacaaaagcg
    37921 atcgctttca cttgggcaaa tcccattaca gctccaccag cagctgcacc aatgcaagca
    37981 gcaacaaatg gttttttcaa tgggatcgca attccgtaaa cagttggttc ggtcactccc
    38041 gcaagtaaaa tcgaagcaaa agcagaaccg gagatcgaac gggtttttga acgtttactt
    38101 cgcagaaata cacctaaagc tgcaccagcc attccaaaat tggctgctcc tgtcaacgga
    38161 acgattcggt cgaacccata agcagagata ttactgatca tgacagggtt tacagcccat
    38221 tggataccaa atattaccaa aatattccaa ccaccagcga tgattgcgcc actgatcggt
    38281 ccgttaatat ccatcatcca agtaattgcg gcagctaaac cttcgccaag gtaaacaccc
    38341 gcaggcccga ataccattaa cgtcaaagga accgtgataa ctaatgaaag taaaggtaca
    38401 aatactaatt gcatattttc gcgaatatat tttttgagga atttttcaac aaaagaataa
    38461 aagaaaatac ctaaaatcgc cggcatgaca gtagacgtat actgcatcag tactacagga
    38521 atccccagaa aatcagtagc tgctttgcca ctgtctgaaa gtaaagaggt cagattcggt
    38581 tcaatcagtg ctgcagcaat gacagccgca atatatccat tcactttaaa cgttttggca
    38641 gcagaaaaag caagaaagat tggcagaaaa taaaagacgg cattcgacgc agcagacaaa
    38701 atatgataag ttccgcttgt ttcactaagc cagccaatcg tagtcgctaa aacaaccaga
    38761 ccttttaata taccagaacc ggcaaacagc ggaatgatcg gcgtaaagat ttccgatatc
    38821 aaaccaaata gtttttcgat cggatttagc ttttctttcg gagctgaatg atcgatttga
    38881 cgcgtcaatt ccttgtaagc aggagccaat ccctgatcca tgattatttc tgcatatcta
    38941 tcgagtaaaa tgaaatactt aatggaaggg atttgctgtt ttaatttttc tgcattggct
    39001 actggctgtt ttttcaattt gatacgaagc cgagtcgcac atggaaggac agaatcaata
    39061 ttgtcttttc ctcccaactg ttcaatcagc atatgaatct gctcggacag taccatctgt
    39121 tcaggattca ttgtcgtttc cattagaaga ccactccact cactaatata atattggcgt
    39181 aaggtgtcat ttcgcctgta cgaataaatg catgacagtt attcaactct tttttcattt
    39241 cttcatgtgg gataaagcta atcggtgtat ctggtaacaa taatttgatt ttttccaata
    39301 tttccgggtt cattgtcttt atttcttccg ccaattcaat cgattgtacc tcaagttctt
    39361 ctaaaacgtt ttctaaaaca gataaaaaac ttggaagacc gtttgagaca gccaaatcta
    39421 ttttttcagt accaaagggg acaggcatgc ctgcatcccc gatacttaat ttatcaaagt
    39481 gccccatttg tgcgattact cgtgaaatat ctgaattgat tacttttcct tttttcatgt
    39541 ttgtttcttc ctctcattgt taattttaga ctgctgattg ttctaaagct tcgcggtaag
    39601 ggatagaagg ttgcgcacca taagactgca cagcaacaga agaggctaga tttccataaa
    39661 ggatcgcttc tttcaagtta ctaaggtctg gctgaagtgt gctgcttaat gcgccaataa
    39721 atgtatcacc tgcggctgtt gtgtcaacgg catccacttt aaaagcaggg acgataccgt
    39781 gttctttttc tgtatgatag aaagcgccgg cactgcctag tgtaatgatt actgttccaa
    39841 tacctaattc atggagtttt tcggctgctg cgaccaaact gttatgatct gtcactctga
    39901 ttcctgtaat gatttctgtt tctgtttcat ttggtataat gatatctgtc ttttcaagta
    39961 gttcagtagg aatcgtttca cgagctggtg caggattcag gatcgttgtt tttcctgcgt
    40021 cttttgcaat agaaaaagct aggatcgtcg catctaagct actttcaaac tgagcaataa
    40081 taaagtcact gtttgtaatc agttgcttgt tttttaggac ttcttgttca tggaaagcat
    40141 tatttgcccc tgcatggatc aagatactat tttctcctgc tgcgtctacc ataatcattg
    40201 cttgtccggt tgtggcgcct tggatttcag ctatcccaga tgtatcgatg ttttcttttg
    40261 tcagtagatc taataactgt tgtccttcgc tgtcagcccc aacgcctcca ataaaagatg
    40321 tccgtgcgcc tgaacgtttg gctgccactg cttggttggc acctttacct ccgccagcgt
    40381 gaaaaatctc atgcgcatgc atcgtttcgc ctggcttggg catgttggtt aaacgaaggg
    40441 tagtgtccat attaatactt ccaattactg taaccgtgtt cattttacaa acctccaact
    40501 atctcattta ttttgatggg aaaattattt tttaaaacgt tttagtaaaa cgttttaata
    40561 gttcgataaa aatatagcac accttgaaag ctatttcaag ggtgcagttg aaaatctttt
    40621 ttctaatcgt acaggtaatc ttttttcttc ccattctttt tctgggaatt ggatccgatc
    40681 taacaataac ttggcagatg tttgtcccaa ttcgaagatt ggttgtgcga tcgttgtcag
    40741 tttcggcttg atatactcgc acatatctat attatcatag ccaataatgc tgtagtcttc
    40801 agggatgctt tttccagctt cctctaatcc gcgatataat ccaaaagcta attcatcatt
    40861 taaagcaaac acaccagttg cagcagaatc aagaagttct gctgtgatct ggtagccacc
    40921 ttgttttgag aactgtgtag gtaagaggat cagttggtcg tgagggattt ggtaaacatc
    40981 aagagcagat ttaaacccct caatccgagc gtggacattt tccggtggat tttcaggata
    41041 gacaagggcg atcgtttgat gtcctagact aagcaagtgc atgcccgcta ggtaaccacc
    41101 gcgaaaatcg tctgttcgta cggcatcact aaatccttca gattttttct gatccaaaac
    41161 aatgaagggc cggccttgtt ttttcaaatt ttcgttgatt gcatctgtag agacagcaga
    41221 agtcgcgata atgaatccat ccacacctcg acgtgtgagc tcggctagat attcgatttc
    41281 tttttgatga tcagaatcag cattacataa tatcgtgacg aaattctgct tatacaagat
    41341 atcctcaatt cctcgcatca gtgtactgaa gaatggattc gtgatatcag gaacgagtac
    41401 accaattgtt ttcgtttcct tggtaatcat ttgtctagca aaatagtcag gctgatagcc
    41461 taattcttct ttggcagcta aaactttctg aacagttttc ggactgaatt tctccgtatt
    41521 tccatttaat atcaaggaga cagttgtgat tgaaacgccg gaaagtttag cgacttcttt
    41581 gattgtgatc ttttttttca ccatgttcat actcctatct aaatcctgtg gttattttcc
    41641 catgaaatca aaaagaagta aatgaaaatc acatgtgcca gtacttattt gctttgttgg
    41701 atagcttcga ttatttgttt cgttgagcgt actcgtgcag ttttcggaaa tatctttttt
    41761 atgaacaatt gatgggattc tttgtcacga tcactgcaag catcttcagc aagcacgata
    41821 tggtacccgt gttgaaaggc atctaatgca gtagcatata caccgtttgt tgtcgctaca
    41881 ccgcttaaaa tgatagtttc gattccacgc cggcgaagtt gaagatcaag agaagtacca
    41941 aaaaaagcac ttggattgta ttttgtgata gtcaacatat tatctgtatc tttcaaagaa
    42001 tccttcaaaa gtaaatcagt gaattctgga ggtacagaag cagtcattcc acccatatca
    42061 gtttgttgac tcaaataagg gaaagtggag atgtctacgt tcactaaaac tttcaaggca
    42121 gcggtacgtt tcaatgttct tactaacaaa tcattgtttg tcagaataga tgctgcagtg
    42181 tgtggagaga gtgtaccgtt atttacgatc tctttttgca tgtcgatcgc gacaaaagca
    42241 gtttttgata aatcaaaaat catattttcc tttaaatcat tcatgatgtt ccttctttca
    42301 aataacaatg ttttttatgt aagtaaatca taaatcgttt atttgtcaac taaattaaag
    42361 gattcctttc tttagtctag agaaattcag tggtaaaata aataagtgtg agaaaacgca
    42421 ttcgatgcat agatatagcg agaaatatcg tgaaaatgga ggaatcagag tgaagtatgt
    42481 cgaagtaaaa aatgaataca aacgatacaa aatgggagaa acgaccatta cagcaaatga
    42541 cgatatttct ttttcaattg aaaaaggcga attagtgatt atacttggtc caagtggagc
    42601 aggaaagtca acgattctta atattttagg tgggatggat acacctgatg aaggtcagat
    42661 cattattgat gacacagata ttgcgcaatt ttcagataaa cagcttactg cttaccgacg
    42721 aacagatgta gggttcgttt tccaattcta caatctcgtt cctaatctaa cagcaaaaga
    42781 aaatgtggaa ttggcaacgg aagtttctcc tgatgcgctt gatcctgtag aggtattgag
    42841 gcaagtgggc ctagctcatc gattgaataa ctttccgtcc caattatcag gaggcgaaca
    42901 gcagagggtt tcaattgctc gtgcactagc taaaaatccc aaacttcttt tatgtgatga
    42961 accgacaggc gcgctggatt ttgaaacggg caagcaagta ttgaaattat tgcaaaacgc
    43021 tagcagacaa cacggcaata ccgttttgat cattacacat aattctgctt tagcgcccat
    43081 cgctgataga gtcattcaca ttaatgatgc taaagtaagg tctgttgaat taaatgatca
    43141 tccttcatcc attgatgaga ttgtatggta gggggatttt atgaaaacaa gaacatatct
    43201 taaagcaggt tttcgcgaaa ttcggcaatc taaaggacga ttcattgcaa tcatccttat
    43261 tgtcatgcta ggaacacttt tatttgtagg tgtcaaaact gctggacctg tcatgcaaaa
    43321 aacgatggat cactatgttg gtacagcagg actttctgat ttgcaaatcg tttctactgg
    43381 tggcttgaca caaaaagata ttgctcaagc agaaaagatc ccagatgcaa aagtagaaac
    43441 aggaaaacag atctattatt ccaatactgg caaaaacgaa gtgattcaaa tattttctta
    43501 taacaaaaat aacaagcaga acaagctaca attaactgat ggaaaactcc cggaaaaacc
    43561 aaaccagctt gtattggatg aaaaagcgga agatgaaggg tataaaatcg gtgatattta
    43621 tcagattgat tcagatgaat tgaaagaaaa agagtatacc atcacgggct ttgtccgttc
    43681 tccattgttc atcaacaatt tggagagagg atacgcgaac gtcggaaatg gtagcgtcga
    43741 ttattttgtc tatttacctg aatccgtttt taaaacagat atttattctg tcttatatct
    43801 tgattttaca aacgtaaaga atttgaatac gtacagcaaa gcttacaaag ataaaatgga
    43861 gcaaaatcaa gaaaaggcag aaaagtattt acaagatcga cccgaagaac gcttagaaga
    43921 gctgaaaaaa tcggccaacg aagaattaga accggcacag caaaaaatag cggatggcaa
    43981 agcacaaaca acgcaagcaa gaacacggtt agaagatgct aaaaaacagt tagcgcaaca
    44041 gtcagcaact atccagcagc ttcctgaagc gcaacgaaca atcgctgttc aaacattatc
    44101 tcaacaagaa gctcaacttg ctgaacaaga aaggcaatta gttgagaaag aacgtgaact
    44161 ggcctctgca gaggaagaat tgaatcgaaa tgcagaaaaa attgaaaatt taactaaacc
    44221 atcataccta tacaatgaac gttcagaaaa tgtcggtttt caagaatttg gtgatctgtc
    44281 tgatcgtatc gctgcgattg ccaatgtttt tcctgttttc tttttcttta ttgccgcatt
    44341 gatcaccttt acgacaatga cacgtatggt agaagaaaac cgcagggaaa tcgggacatt
    44401 aaaggcgtta ggttatacga aagttgaaat cgcaaaaaaa tatgcaatct atgcaagtct
    44461 agcctcaggg ataggtattg ttttaggaac gatattagga acgaatcttt tgccaaggat
    44521 catttttgag ctgtctaacg agcggtatga tattgggagt gcgcttattt tttatgactg
    44581 gccgccgatt ttacaagcag ctgccgcatt cttcattgcc gcatttggcg ctgcaatgat
    44641 cgttttattc aaagatttaa gagaacggcc tgctgcattg cttcaaccga aagcaccgaa
    44701 gccagggaaa cgcatttttc ttgaatacat cactccgctg tggtcacgcc ttagctttaa
    44761 tcaaaaaatc agttaccgaa atctcttccg atataagtca agaatgctta tggcgattat
    44821 cggaattgct ggatgtgccg ggctgatggt tgcaggcgtg ggattgaaag attctctaag
    44881 ttctgtttca gccaaacaat ttggaacgat cattgactat caagcaatcg ttacattgga
    44941 ttcatccacg gaagatagtc gtcaaaaagc agcggatatg ctgcagaagc aagaaaaagt
    45001 aaatgagatc ctagctattc atactcaaac cgtagaattg agaaaaaaag gacatgcttc
    45061 gcaaagtctg acagtgatcg taccaagtaa aacagctgat ttggatcgtt ttatccattt
    45121 aactgatctt gaagagaacc cacgctcctt gcctaaaaca ggaatcgcta ttacgcaaaa
    45181 agtagcagaa ctatttgact tatctacagg agatcatatt tcgctttatg atgaagatca
    45241 aaaagaatgg aaagtcgaag taaaagaaat cgtgaaaaac tatctaggta gctttattta
    45301 tatgtcccct gaatattatg cttcagttac tgatcaatcg atgatggata atgcattttt
    45361 gctgcgtaca gataagatga caacaaaaga agaagaaatg ctttccgaga aactgcttgc
    45421 cacagacgct gtgacaaata cgtctttcat gtccaaacaa atcgaaacgc aagaagaatc
    45481 aatggcgaac ttagactcgg ttgtcttaat ctttgtcgta ttatcaggac tgctggcttt
    45541 tatcgtcttg tataatctaa ctaatatcaa tatttccgag cgtgtacgcg aattatctac
    45601 aataaaagtc cttggatttt ttgataaaga agtcacgatg tacattgttc gagaaaatat
    45661 catctttact cttttaggaa tcatcggagg atttggtgta ggatatatat tgactgactt
    45721 catcttgcaa caagcgtcta tggagaatgt catctttcca ttagtgatca catggtgggc
    45781 ttatgtctta tcagcaggat taactatcgt atttacggtt attgtgatga ttgttaccca
    45841 tttcaaatta aagcatatcg atatgattga tgcgttgaaa tcgaatgaat aaaaacagag
    45901 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggcaaaa tagtttttca
    45961 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacca tgaatcagag
    46021 gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat tagctcctca
    46081 tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac catttataga
    46141 agaaacaaga atattttgct attttaaaca agaggataga caaatacatg tctgatctct
    46201 tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta caaacgcttt
    46261 cggttcagtt ataataataa atgataactt gtatatataa gttgtggtag gtaataaatc
    46321 agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc aaatctatcc
    46381 caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa tcaaagaaaa
    46441 actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta tttatccaag
    46501 ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc ctatctttgg
    46561 tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga tagaaatcat
    46621 gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa aagcactagc
    46681 aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg attgggtatc
    46741 aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt atttgcactt
    46801 atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag aggaactatt
    46861 caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg atgttatcaa
    46921 tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca aacctgaata
    46981 tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa cgtttgggac
    47041 ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa attgtatctt
    47101 gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc atttgaaagt
    47161 cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag aattaaagcg
    47221 actatttcat acatggggag aaaagatgag tgagggtaac ggctggaatg ctctattttt
    47281 taataatcat gatcagccac gtgcgctcaa tcgatttgta gctatcgatc agtatcgaaa
    47341 acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc cctatattta
    47401 tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg actatattga
    47461 cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag aaaaagaagc
    47521 gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc aatggtcaga
    47581 cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag gcaagctaga
    47641 agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct attacaagaa
    47701 attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc atgcttatga
    47761 agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc agctgcttgt
    47821 attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat tcacagaagc
    47881 caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg agctaaaacc
    47941 ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc gttcctacga
    48001 gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt cagaaaatga
    48061 agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt tgaatttacg
    48121 gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa aaataacgcc
    48181 cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa tcggatggat
    48241 ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa gttgtacggc
    48301 aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg caatgatcaa
    48361 acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca acccagcaga
    48421 aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc agtagactgc
    48481 tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt gttgattagc
    48541 aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg ctaaaactga
    48601 ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac cagtaaataa
    48661 agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg cgccactttc
    48721 ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag aagcgaaaaa
    48781 ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac tctcttgaaa
    48841 atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt caagaaaagg
    48901 aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa cgataatgac
    48961 tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca aaagataata
    49021 caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc cgacaggctt
    49081 gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga taactattcc
    49141 taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg ttgtatctgt
    49201 ttcttttttc actctttttc ctcctttatc tataacttca ttatagacaa aagcgggtat
    49261 aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata taaactttct
    49321 catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa attaaatcct
    49381 gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc aaatgaaaga
    49441 agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc atgctggttt
    49501 tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt aaagaaaaat
    49561 tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc gtcggggatg
    49621 aaggagaagt agagttattg attccagcag ggactgattc ccacgattat gaaccttctg
    49681 ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat gaaaacatgg
    49741 aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat acgataaaag
    49801 cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat gaccatgaac
    49861 acggggaaga aggccataca catgagctag acccacatgt atggctagct ccaagtttag
    49921 caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca gaaaaacagg
    49981 aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc catcagcttt
    50041 atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat gctgcattca
    50101 actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt tcttctagtg
    50161 aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa catggaatca
    50221 actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta gcaaacgaag
    50281 caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa caaatagaaa
    50341 atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa aaaacgacag
    50401 agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc gtctacaacg
    50461 gttattttga agacgaggat gtacaagatc gctctctttc agattgggca ggaacttggc
    50521 aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa tataaagaaa
    50581 aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag ggctatcaaa
    50641 cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt aagaatggaa
    50701 caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac gaggcaggaa
    50761 accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct tttagatata
    50821 ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat ttatatgcag
    50881 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
    50941 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
    51001 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
    51061 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
    51121 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
    51181 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
    51241 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
    51301 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
    51361 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
    51421 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
    51481 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
    51541 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
    51601 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
    51661 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
    51721 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
    51781 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
    51841 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
    51901 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
    51961 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
    52021 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
    52081 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
    52141 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
    52201 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
    52261 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
    52321 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
    52381 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
    52441 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
    52501 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
    52561 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
    52621 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
    52681 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
    52741 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
    52801 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aagaaagcgc gtaaaccaat
    52861 tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat agcaattaga
    52921 gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga aatcagaatg
    52981 actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat acaaattgtg
    53041 taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc ccattgattt
    53101 ttaccaaatt atagcacaat tggcgtatta tttttttaat ttttcaggga ttttgaattc
    53161 agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag ttaattgttt
    53221 aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt gtaataggaa
    53281 gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag taagaagctg
    53341 agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct gatcgccttc tttaataatg
    53401 ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca tagcgaagca
    53461 gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata gtcaattgca
    53521 gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc agacaaacga
    53581 attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa gaaagaaagg
    53641 tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg accgctagtt
    53701 ttccaagatt attcaataaa acttctaaga caaattttac ttggacatac accatatctt
    53761 cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact ttatctttca
    53821 tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg tcttttgttt
    53881 cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc ttcgttttcc
    53941 aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta tgacaagttt
    54001 gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt gatggaggga
    54061 ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt aaatctgatt
    54121 tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt gtgatgatac
    54181 gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac tggcagttag
    54241 ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga gagcataaag
    54301 aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa aacagctttt
    54361 catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact gtttatttga
    54421 aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag gagttacttt
    54481 tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt ttatttctat
    54541 aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga taattgtgat
    54601 cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc ttgcccaaga
    54661 tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc cgctatttta
    54721 ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca aacgcttttt
    54781 ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca tctacgaaga
    54841 tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag aagtaaagca
    54901 gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc cacaaaagaa
    54961 accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga gaatcaaaaa
    55021 aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct actataaaga
    55081 actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca aaatagtgat
    55141 cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa tcgtacaaga
    55201 aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga tccgctacac
    55261 tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa actaagcaaa
    55321 ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt tgattcattg
    55381 agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg tctgagtgag
    55441 ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa acagatgatg
    55501 acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta gatagtggaa
    55561 aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt tcgttgctac
    55621 cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga ttttatcaaa
    55681 agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg cgggatgctc
    55741 atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata gaacaaacaa
    55801 gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg ttagaagaat
    55861 ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc attctccgtc
    55921 aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa aagcaccata
    55981 ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa gtcctattgt
    56041 acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat atttttgtct
    56101 tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac taaaatcata
    56161 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
    56221 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
    56281 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
    56341 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
    56401 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
    56461 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
    56521 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
    56581 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
    56641 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
    56701 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
    56761 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
    56821 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
    56881 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
    56941 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
    57001 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
    57061 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
    57121 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
    57181 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
    57241 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
    57301 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
    57361 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
    57421 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
    57481 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
    57541 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
    57601 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
    57661 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
    57721 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
    57781 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
    57841 cttttctcct aaatatcaaa atatatccgt tgcagattat attttgtcga taagccccaa
    57901 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
    57961 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
    58021 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
    58081 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
    58141 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
    58201 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
    58261 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
    58321 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
    58381 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
    58441 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
    58501 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
    58561 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
    58621 gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat caaaatagag
    58681 aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg atttttttta
    58741 tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg gattataatt
    58801 aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa
    58861 agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga
    58921 accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga
    58981 tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac
    59041 tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt
    59101 gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc
    59161 tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa
    59221 ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa
    59281 gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag
    59341 aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag
    59401 cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct
    59461 gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc
    59521 ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc
    59581 ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt
    59641 gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc
    59701 tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag
    59761 ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta
    59821 gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat
    59881 gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga
    59941 aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc
    60001 tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac aattacttgt
    60061 ctctctgata tgatataaaa gttgctacgg cagccaaaaa c
//
LOCUS       DACEMK010000005        51396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEMK010000005 DACEMK010000000
VERSION     DACEMK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.005"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            290..1135
                     /locus_tag="IW715_000255"
     CDS             290..1135
                     /locus_tag="IW715_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1020884.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IW715_000256"
     CDS             1416..2636
                     /locus_tag="IW715_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1020885.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IW715_000257"
     CDS             2633..5353
                     /locus_tag="IW715_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1020886.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IW715_000258"
     CDS             5337..6281
                     /locus_tag="IW715_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1020887.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IW715_000259"
     CDS             complement(6350..7360)
                     /locus_tag="IW715_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1020888.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IW715_000260"
     CDS             complement(7540..7884)
                     /locus_tag="IW715_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1020889.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IW715_000261"
     CDS             complement(7877..8311)
                     /locus_tag="IW715_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1020890.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IW715_000262"
     CDS             8449..9186
                     /locus_tag="IW715_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1020891.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IW715_000263"
     CDS             9179..10390
                     /locus_tag="IW715_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1020892.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IW715_000264"
     CDS             10597..11376
                     /locus_tag="IW715_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1020893.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IW715_000265"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IW715_000265"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1020894.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IW715_000266"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IW715_000266"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1020895.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IW715_000267"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IW715_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1020896.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IW715_000268"
     CDS             14978..16027
                     /locus_tag="IW715_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1020897.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IW715_000269"
     CDS             16024..16302
                     /locus_tag="IW715_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1020898.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IW715_000270"
     CDS             16277..16747
                     /locus_tag="IW715_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1020899.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IW715_000271"
     CDS             16764..17066
                     /locus_tag="IW715_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020900.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IW715_000272"
     CDS             17083..17454
                     /locus_tag="IW715_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1020901.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IW715_000273"
     CDS             17426..17785
                     /locus_tag="IW715_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020902.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IW715_000274"
     CDS             17957..18964
                     /locus_tag="IW715_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1020903.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IW715_000275"
     CDS             18989..20173
                     /locus_tag="IW715_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1020904.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IW715_000276"
     CDS             complement(20610..22193)
                     /locus_tag="IW715_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1020905.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IW715_000277"
     CDS             complement(22771..22914)
                     /locus_tag="IW715_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1020906.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="IW715_000278"
     CDS             complement(23137..23616)
                     /locus_tag="IW715_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1020907.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="IW715_000279"
     CDS             complement(23689..23973)
                     /locus_tag="IW715_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020908.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="IW715_000280"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW715_000280"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="IW715_000281"
     CDS             complement(24317..25603)
                     /locus_tag="IW715_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1020909.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="IW715_000282"
     CDS             25741..26412
                     /locus_tag="IW715_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1020910.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="IW715_000283"
     CDS             26378..26866
                     /locus_tag="IW715_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1020911.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="IW715_000284"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="IW715_000284"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1020912.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="IW715_000285"
     CDS             27827..28591
                     /locus_tag="IW715_000285"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1020913.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="IW715_000286"
     CDS             28824..31037
                     /locus_tag="IW715_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1020914.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="IW715_000287"
     CDS             31048..31494
                     /locus_tag="IW715_000287"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1020915.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="IW715_000288"
     CDS             31665..32924
                     /locus_tag="IW715_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1020916.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="IW715_000289"
     CDS             complement(33013..33960)
                     /locus_tag="IW715_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1020917.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="IW715_000290"
     CDS             34354..35646
                     /locus_tag="IW715_000290"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1020918.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="IW715_000291"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="IW715_000291"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1020919.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="IW715_000292"
     CDS             37539..38414
                     /locus_tag="IW715_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1020920.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="IW715_000293"
     CDS             38680..40563
                     /locus_tag="IW715_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1020921.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="IW715_000294"
     CDS             complement(40638..40922)
                     /locus_tag="IW715_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1020922.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="IW715_000295"
     CDS             complement(40912..41646)
                     /locus_tag="IW715_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1020923.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="IW715_000296"
     CDS             41775..42398
                     /locus_tag="IW715_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1020924.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="IW715_000297"
     CDS             42590..43711
                     /locus_tag="IW715_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1020925.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="IW715_000298"
     CDS             43718..46846
                     /locus_tag="IW715_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1020926.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="IW715_000299"
     CDS             46865..50059
                     /locus_tag="IW715_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1020927.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="IW715_000300"
     CDS             complement(50205..50912)
                     /locus_tag="IW715_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1020928.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16844 a         8966 c        10651 g        14935 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttca
//
LOCUS       DACEMK010000006        47107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEMK010000006 DACEMK010000000
VERSION     DACEMK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.006"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(137..1528)
                     /locus_tag="IW715_000301"
     CDS             complement(137..1528)
                     /locus_tag="IW715_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1020929.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IW715_000302"
     CDS             complement(1741..2127)
                     /locus_tag="IW715_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1020930.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="IW715_000303"
     CDS             complement(2183..2806)
                     /locus_tag="IW715_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1020931.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="IW715_000304"
     CDS             complement(2870..3538)
                     /locus_tag="IW715_000304"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1020932.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="IW715_000305"
     CDS             complement(3550..3990)
                     /locus_tag="IW715_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020933.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="IW715_000306"
     CDS             complement(4049..4720)
                     /locus_tag="IW715_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1020934.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="IW715_000307"
     CDS             complement(4905..5303)
                     /locus_tag="IW715_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1020935.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="IW715_000308"
     CDS             complement(5300..6073)
                     /locus_tag="IW715_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1020936.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="IW715_000309"
     CDS             complement(6070..6753)
                     /locus_tag="IW715_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1020937.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7807)
                     /locus_tag="IW715_000310"
     CDS             complement(6920..7807)
                     /locus_tag="IW715_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1020938.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7804..8673)
                     /locus_tag="IW715_000311"
     CDS             complement(7804..8673)
                     /locus_tag="IW715_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1020939.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8810..10096)
                     /locus_tag="IW715_000312"
     CDS             complement(8810..10096)
                     /locus_tag="IW715_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1020940.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10369..10947
                     /locus_tag="IW715_000313"
     CDS             10369..10947
                     /locus_tag="IW715_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1020941.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10947..11876
                     /locus_tag="IW715_000314"
     CDS             10947..11876
                     /locus_tag="IW715_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1020942.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11873..13609
                     /locus_tag="IW715_000315"
     CDS             11873..13609
                     /locus_tag="IW715_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1020943.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="IW715_000316"
     CDS             complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="IW715_000316"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1020944.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14505..15014)
                     /locus_tag="IW715_000317"
     CDS             complement(14505..15014)
                     /locus_tag="IW715_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1020945.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="IW715_000318"
     CDS             complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="IW715_000318"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1020946.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16315..17406)
                     /locus_tag="IW715_000319"
     CDS             complement(16315..17406)
                     /locus_tag="IW715_000319"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1020947.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="IW715_000320"
     CDS             complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="IW715_000320"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1020948.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="IW715_000321"
     CDS             complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="IW715_000321"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1020949.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="IW715_000322"
     CDS             complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="IW715_000322"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1020950.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21369..22343)
                     /locus_tag="IW715_000323"
     CDS             complement(21369..22343)
                     /locus_tag="IW715_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1020951.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22324..24207)
                     /locus_tag="IW715_000324"
     CDS             complement(22324..24207)
                     /locus_tag="IW715_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1020952.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24225..26450)
                     /locus_tag="IW715_000325"
     CDS             complement(24225..26450)
                     /locus_tag="IW715_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1020953.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26565..27599
                     /locus_tag="IW715_000326"
     CDS             26565..27599
                     /locus_tag="IW715_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1020954.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27666..28679)
                     /locus_tag="IW715_000327"
     CDS             complement(27666..28679)
                     /locus_tag="IW715_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1020955.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28757..28945)
                     /locus_tag="IW715_000328"
     CDS             complement(28757..28945)
                     /locus_tag="IW715_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1020956.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29385..29513)
                     /locus_tag="IW715_000329"
     CDS             complement(29385..29513)
                     /locus_tag="IW715_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1020957.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="IW715_000330"
     CDS             complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="IW715_000330"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1020958.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31000..32055)
                     /locus_tag="IW715_000331"
     CDS             complement(31000..32055)
                     /locus_tag="IW715_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1020959.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="IW715_000332"
     CDS             complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="IW715_000332"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1020960.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="IW715_000333"
     CDS             complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="IW715_000333"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1020961.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="IW715_000334"
     CDS             complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="IW715_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1020962.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="IW715_000335"
     CDS             complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="IW715_000335"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1020963.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="IW715_000336"
     CDS             complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="IW715_000336"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1020964.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39817..41376
                     /locus_tag="IW715_000337"
     CDS             39817..41376
                     /locus_tag="IW715_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1020965.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41487..43418)
                     /locus_tag="IW715_000338"
     CDS             complement(41487..43418)
                     /locus_tag="IW715_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1020966.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="IW715_000339"
     CDS             complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="IW715_000339"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1020967.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44347..45099)
                     /locus_tag="IW715_000340"
     CDS             complement(44347..45099)
                     /locus_tag="IW715_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1020968.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45259..45468)
                     /locus_tag="IW715_000341"
     CDS             complement(45259..45468)
                     /locus_tag="IW715_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020969.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45479..46807)
                     /locus_tag="IW715_000342"
     CDS             complement(45479..46807)
                     /locus_tag="IW715_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020970.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        13139 a        10182 c         8398 g        15388 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
     7801 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
     7861 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
     7921 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
     7981 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
     8041 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
     8101 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
     8161 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
     8221 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
     8281 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
     8341 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
     8401 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
     8461 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
     8521 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
     8581 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
     8641 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
     8701 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
     8761 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
     8821 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
     8881 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
     8941 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
     9001 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
     9061 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta
     9121 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
     9181 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
     9241 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
     9301 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
     9361 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
     9421 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
     9481 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
     9541 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
     9601 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
     9661 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
     9721 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
     9781 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
     9841 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
     9901 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
     9961 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    10021 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    10081 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    10141 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    10201 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    10261 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    10321 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    10381 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    10441 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    10501 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    10561 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    10621 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    10681 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    10741 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    10801 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    10861 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    10921 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    10981 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    11041 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    11101 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    11161 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    11221 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    11281 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    11341 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    11401 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    11461 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    11521 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    11581 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    11641 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    11701 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    11761 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    11821 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    11881 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    11941 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    12001 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    12061 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    12121 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    12181 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    12241 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    12301 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    12361 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    12421 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    12481 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    12541 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    12601 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    12661 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    12721 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    12781 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    12841 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga
    12901 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    12961 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    13021 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    13081 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    13141 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    13201 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    13261 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    13321 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    13381 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    13441 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    13501 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    13561 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    13621 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    13681 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    13741 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    13801 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    13861 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    13921 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    13981 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    14041 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    14101 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    14161 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    14221 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    14281 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    14341 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    14401 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    14461 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    14521 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    14581 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    14641 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    14701 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    14761 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    14821 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    14881 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    14941 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    15001 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    15061 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    15121 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    15181 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    15241 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    15301 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    15361 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    15421 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    15481 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    15541 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    15601 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    15661 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    15721 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    15781 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    15841 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    15901 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    15961 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    16021 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    16081 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    16141 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    16201 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    16261 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    16321 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    16381 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    16441 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    16501 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    16561 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    16621 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    16681 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    16741 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    16801 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    16861 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    16921 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    16981 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    17041 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    17101 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    17161 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    17221 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    17281 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    17341 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    17401 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    17461 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    17521 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    17581 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    17641 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    17701 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    17761 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    17821 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    17881 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    17941 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    18001 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    18061 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    18121 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    18181 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    18241 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    18301 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    18361 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    18421 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    18481 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    18541 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    18601 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    18661 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    18721 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    18781 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    18841 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    18901 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    18961 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    19021 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    19081 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    19141 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    19201 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    19261 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    19321 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    19381 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    19441 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    19501 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    19561 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    19621 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    19681 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    19741 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    19801 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    19861 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    19921 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    19981 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    20041 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    20101 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    20161 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    20221 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    20281 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    20341 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    20401 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    20461 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    20521 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    20581 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    20641 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    20701 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    20761 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    20821 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    20881 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    20941 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    21001 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    21061 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    21121 tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt
    21181 tttatgaaac tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt
    21241 ggttttattg aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt
    21301 tcatgacata tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct
    21361 aaagattgct actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa
    21421 ggtgactcaa catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa
    21481 atcgtaagaa ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt
    21541 tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc
    21601 aacgaaaaat cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct
    21661 acccatttcg tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg
    21721 agtcctgcca ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc
    21781 cctgataggc cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc
    21841 aatgcgggta attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta
    21901 ccctctccag cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc
    21961 agtgtaaaaa tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact
    22021 ggagcaatcg gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt
    22081 ggatacaatc cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca
    22141 ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg
    22201 tttttattga gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc
    22261 gatttgacac ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga
    22321 cttttattta ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg
    22381 tccatctgca aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa
    22441 gtcaccgcca tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg
    22501 atctttaaaa tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat
    22561 gtaagaatcc attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa
    22621 cggtttttcc gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca
    22681 gctttttaca tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg
    22741 gacaagtcga tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc
    22801 cgcataagat tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc
    22861 gaaattcgtt tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac
    22921 gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt
    22981 ttccgccatc acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg
    23041 gtaatgagat gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc
    23101 catatcttct atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc
    23161 gttgataatc atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg
    23221 aaaaccgaag gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa
    23281 ttcaagctta tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc
    23341 gatgcgaatc gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc
    23401 aggatctagt atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc
    23461 tacttcaagt ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc
    23521 taaatggttt tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa
    23581 aaagtcctga tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat
    23641 attttcttct tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc
    23701 atacccgata aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg
    23761 aatcgagatt tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc
    23821 aaccggattg tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt
    23881 gaaaggggga cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat
    23941 cttgtcataa tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc
    24001 atcaaaatct tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa
    24061 acgccatgat ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt
    24121 tgcataacaa acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc
    24181 atctaaccat tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa
    24241 tgaagcagtt ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc
    24301 aattcggccc ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct
    24361 aaccccatca gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa
    24421 aacacaatag cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca
    24481 aaaggactcg ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag
    24541 gatataattt ggtcttttac ttgctttttt tcttcaggag acagctttgt caaatccagt
    24601 tcataaccaa aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg
    24661 tcggtctgat gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca
    24721 aggcttgttc catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat
    24781 gactgcggca tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct
    24841 tcccataaga tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc
    24901 atataacgat gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct
    24961 gaaagcgaac ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt
    25021 ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt
    25081 gatctggaag cagaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga
    25141 tacagttcag aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct
    25201 ttgctatgag cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg
    25261 aaccagtctc ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat
    25321 acgaacattt cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta
    25381 ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga
    25441 tgacgacctc ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc
    25501 aatccctgac ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc
    25561 aattcccacg aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga
    25621 tctttttcaa gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct
    25681 ccagtatttt ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg
    25741 ctcgttcctc gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc
    25801 tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg
    25861 gcaaagtcta gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt
    25921 cttatcttta cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg
    25981 aaataaatcg ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg
    26041 tccatcacat aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg
    26101 ctgtcatatc tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc
    26161 gttcgaaagt ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga
    26221 gaccggtcag gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt
    26281 ttattgtctt tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt
    26341 tcttctatct ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt
    26401 tcatcaaata agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc
    26461 ccaacaaaac ggaaaccgtt tacttttttt tataattcct gtatactgtt tactaaacaa
    26521 ttttgtcaat aaaaattttt tatttttttg aggagtgtgt aaaaatggtt ggaattagag
    26581 atgtcgcacg aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg aataacgatc
    26641 agacgatcca tgtggctcag actactcggc aacggatagt agaggcagct gctgcgttgg
    26701 attatgacat cagtactcgt aaatatatca aaaagagaat gccttcgatt ggtgtgatct
    26761 ctacgatcgg caaagcgagc gaagaacagg atatctatta caaacatttg cgagtaggga
    26821 tggaagaaga agctcgacgt ctgcatttag ggatgaatcg aatctataat ctatctgata
    26881 atcctaaaca atgggaagat ctagatcagt taggtgcggt gatcgttgtt gggactgtta
    26941 cgaaagaatc gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc gtggataacc
    27001 ctgacatcca gcaagaggtc gatatggtgt atgcagatct tgaacggatg actaaaaccg
    27061 tactggaatt gttcttagaa aatggtcata aaagaatagc ctatatcggg ggatataaag
    27121 tcgatatgga tgaattcggt gggaagcaga tttctataaa tgaaaagagg atgcgtgcct
    27181 atcagcattt tatgatcgat catcacttag aaaaatatat gtcttatcat ctaggtggct
    27241 ggacagaaaa acaaggtgag gtacttgcga ctaatctatt agaaaatgaa tcccctcttc
    27301 ctactgccat tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg gcattgaaac
    27361 aaaaaaatat ccaaattgga gaagagattg ttgtagcaag ctttgataat atcgagacaa
    27421 tcgcaaacct ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag
    27481 cagctgttcg aatggcattg gagcgaattg acggtatacg ggacgaacca atcattttga
    27541 cttatcctgc acgattgatt gttagggaaa gttttatccc aagaacaaaa aggtattgaa
    27601 agagaggttg tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg ctttttcaca
    27661 acctcttact tatacttatt cacaaaatcc aatgtctgct ggtaacaatt ttgcgattct
    27721 tttagcgtgt aatcaaattg gtattcatgg gtaatctctt tttttgtatc tgtataaaac
    27781 aaggattgca cgggtacctt taattctgtc aaccgatttt ctaatgcttg accttgctcc
    27841 tggaatgagt atgcatttcc gtcagttata tacgttggcg gaaattcctt agaaacatgg
    27901 tcagccacac tagcttcttt caattgctca ctcgttttcc aatcttttgt tccaatcaac
    27961 gaccacgcaa cggttttgac gaaaaacttc ataaatcgat catccgaatg ctgctgtgct
    28021 gttagcttca aatcaacagg tccgcaataa gaaatggttc ctttcagact atcttttgga
    28081 agtagctgtt tgattttcat ttgtttagca tacgcatcat tcgtttgtat agctgcatat
    28141 tgcaatgcga tttgtgaacc tgcactatct ccaccaaaaa ataattgttc catgtctagc
    28201 aatttgtcgt ctttttcttc agctaataaa tctttgatca gttcattcac ttgaagaact
    28261 tgattagggt attctgaatc aggcgctaat tcataattca tcgcaaccac tgcgatgttg
    28321 gcgtctgaaa ctaattttgt cgcaaattct ttgacacccg atttatctcc gccgacgaat
    28381 cctccaccat gtacccaaaa tagaacagga acaggcccct tgctgttttt tggatagtag
    28441 atgtcataag tattctcttt atacttggac tcatacgtca aatctgtttt tttcgccacg
    28501 tttttttctg cttgatcgaa cgttttttta tcagtgattt gaacagtatt tgaaaacata
    28561 tggttgatca agaatgctcc aggtctgggg gaaagctgga aggcaagaaa gattgcaaac
    28621 gcgatcccaa gaatgattcc gaagaaccat ccgataaatt taagaatttt ctttttcata
    28681 aggctccctc agtagttagt attaagcatt ctgattatac catagtgtta tttcctatca
    28741 agctctccta gatgatttaa tgattttccc caaacttctt ctgcaatgct tttgacaaaa
    28801 gaaatttttt ccattgttct tcaaacaaaa cattttcttc ttctgttgaa gcaaatccac
    28861 attgaggact taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg tagatccggt
    28921 caatcacttg ctggtgctct tccatttcgc catttttcgt tacagtcaaa atctaagaca
    28981 actaatttat cttcagagac tttctctaaa agaacaaaac tcccagcacg ttcactatcg
    29041 tattctaaat aataggcacg gacattttct tgagtaaaat aatgggtctg ctacgtcgtc
    29101 atcccccctg atgccgccaa aatcgaataa taatttccgc gtcaaacatg tatatttagt
    29161 gttgaatcat ttgagaatat gaaaataaaa atctttttga tcaatcagtt aatcaaattg
    29221 aaataacgaa aaatctgaga cagaatgacg ttcattctga cccagatttg attttgcaca
    29281 actcaacagt ccttattggc aaatcgccaa aataatgtag actaactaaa aagactaaga
    29341 aacaataaca aaagttaaca cttcttagtc tttctttagc acgtctattt attaaagatt
    29401 ttagggcctt ttctcatgtt tttacgatca ttcttaggtg tttccatttg tttggatcgg
    29461 cctccacctt gtttcttttt gatcaattct ttcatttttt ctttttggtc catttttatt
    29521 ctcctttaac gaatgaagcg actaattcga tatgatgggt ttgcgggaaa agatctacag
    29581 gctgaacttt cttcacggca taacctgctt ctgcaaataa cttagcatct cttgcaaaag
    29641 ttgccggatt acatgagata tagacgattc gttctggtga catttcgatt gctgattcaa
    29701 taaaatgatg atctaatcct tttcgtggag ggtcaactac gatcacagat ggtttgatcc
    29761 ctttttcttg ccattttttc ataacttttt ctgcttttcc gacttcataa tgaacatttt
    29821 cgattttatt atttaaagca ttgaactgtg catcttcaat tgcttctgga ataacttcca
    29881 tcccatacac cttatccact ctgtctgcta aagacaagcc aatcgtacct attcctgaat
    29941 aagcgtcgat cacaatgtct tttttatcta actcggcaaa ctcgatggct gtttggtaca
    30001 gatactctgc ttgttgagta tttacttgat aaaatgactt tgctgagatt cgataggttt
    30061 ttcctaatag ctgatcttcg atatagtctc tgccatataa aactttttct ttgtctccta
    30121 aaatcacatt tgttttttct tcattgatat tttggatgac cgaaaccact tctggcaatt
    30181 cttcatgaat agcttcagca atttccttgg atttaaagaa tttttcttta cgagtaacta
    30241 aaacgaccat catttcatgg gaataatgac cacgacggat aatgatgtga cgaagctgtc
    30301 cttcattttt ttcttcatta tatcctcgta cttggaaccg ttgcaagata tctctgacta
    30361 ctttgattgc ttgatcgatg acaggatctt ggatataaaa atcttcgata gggactagat
    30421 catggctatt ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg cgaacaggga
    30481 tctgtgcttt attacggtag ccgaatggtt ctgccattcc gaccgttgga agtacaggaa
    30541 cttctggcat cttagcaatc ttcgccatta catgctctac ttgtttttgt ttgaagacaa
    30601 gctgttgttc atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt aatgcattgt
    30661 tttctacagg ctgacgatca ggtgattgtg taataaatgt ttcgactttt ccatatccaa
    30721 acttattgcc tgcttttact actagtattt ctacttcttc tccagggagt gcattttcga
    30781 taaataatgg ataaccatcg atttttgcta cccccattcc ttcataactc aagtcgacga
    30841 tttcgactgt atatcgttcg tttttcttta ctatttgctg ttctgtcata ttttgctcct
    30901 ttaagcctat ttttcaatcc tactgaactc tactataaat cagctaatta cacaactaaa
    30961 accggcactg accttactac gttcagcacc ggcttttgat taattctttt gattaggtgt
    31021 ttcgtctgct atttttccgt caccgtcgat atcttcatcc gttaaacgtt cgacttcttt
    31081 tacgaattct ttgctgacaa cctctaattc ttcttcatct gtacctgtga ttgcttcatc
    31141 aggaatctga tcggtattgg caaagaactc aatgtgttga tgaagatttt ggaaatgcat
    31201 cggtgcttcc ccgccgtatt caccatctaa gttgatcatt actggtcgat tgatatcatc
    31261 ttctgtttca acgaacaaac tgcttgtttt ggtatagatt attcttggat catcgacatg
    31321 tcgaccgcca ttcagcatga gtgctgcaat atgcaagatc tcaaagatat ttgctgtctt
    31381 cacaatgatt aaagaaaact tcccatcatc caatttggca tctggcgcga tctgttcgaa
    31441 tcctccaaca gagttcgtca gccctaggaa aaacatcgaa gcatttccga tatattcccc
    31501 ttcatcatac ttcaagcgca ttttgattgg ctttactcga gggagcatct ctgctccttt
    31561 cgcaagataa gcaagataac caaaaatact ctttagttct gatggaactt cataagtcaa
    31621 ttctgtcaaa tagccacctg cagcgatatt gatgaagtaa ctgttatcag aaaggccgat
    31681 gtccattttt acggtttggt gtttttttat cacttctgct gctgctttaa tattatctct
    31741 aggaattttt aatgctcgag catagtcgtt cgttgtacca gctgggatga tggccatttt
    31801 aggacggtgt tcaagcggag cgattccatt aaccacttcg ttgattgtgc catcgccacc
    31861 agctgccact actagatcaa aacctagttc tgcgactcta catgcttcat tcttggctga
    31921 atctggttct ggtgtagttg cataggcgct ggcttcaaat cctgcttctt ctaatgcctg
    31981 taggatatcc gccaaatttt ttttcaataa ttcttttccc gaggtggggt tataaattac
    32041 gcgtgctttt ttcattttca gcctctatcg tttcgctaat tcatctttaa gtagcttatt
    32101 cacgacacca ggatttgctt gtcctttagt tgctttcatg atttgaccga ctaggaagcc
    32161 aaccgcacga tcttttccat ttttgaaatc atcgacagat tgttggttgt tatccaaaat
    32221 ttcattaatg attggcagta gtttgttcgg atcagataat tgcacccaac catttttttc
    32281 aactacttct tgggcttctc cgccatttgt gatcaattca cggaagactt ttttggcgat
    32341 ttttgagctg atcgttccat cggcgatcaa acgaatcatt cctgctaagt tctcaggtgt
    32401 caattttgtc tcagctaatt ccagtttctc actgttcaag taagcagaaa cttcacccat
    32461 caaccagtta gatgcttgct tggcatctgc accattttcc aaaacagctt caaaaaaatc
    32521 agacatttct tttgtaagtg tcaataccat tgcgtcatac tctggaagtc caaactcacg
    32581 gatatagcgt tcgcgacgag aagctggcat ctcaggcaaa ctagctttta ctttttgaat
    32641 ccattcgtca tcgatcacga atttcgggat atcaggctct gggaagtaac gataatcact
    32701 agacccttct ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa aacggcgtgt
    32761 ttcttgttgg atttctcctc ctgaaagaag aacttttgct tgacgttttt cttcaaaagc
    32821 taaacctttt ttcacgaaag ataaagagtt caagtttttc aattctgctt tcgtaccgaa
    32881 ctcttcttgt ccataagggc gaagagaaat attggcatca cagcgcatag agccttcttc
    32941 cattttcaca tcgctgactt ctgtaaattg gataattgat cgcaatgctt ctagataagc
    33001 gtatgcttct tctggtgaac gcatatcagc ttcagaaacg atttcgatca aaggcgtgcc
    33061 ttgtcggttc aaatcaacgt aagaataacc gccaatacca tgcatgtttt ttccagcatc
    33121 ttcttctaaa tggacacgtt cgatccggat ttttttcttt ttaccttcta cttcgatttc
    33181 gatccatccg tcatgaccga ttggttgatc aaattgggaa atttgatatg cttttggatt
    33241 atctgggtaa aagtaatttt tccggtcaaa atgagtatct ttagagattt cacagtttaa
    33301 tgccaaagct gctttcatcc cgaattccag cgcagcttta ttcattactg gcagcacgcc
    33361 tggataaccc cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa aatgagctgg
    33421 cgctggagag aaaatttttg agttggtttt taactcgacg tggacttcta gtccaatgac
    33481 tgtttcaaaa ttcattcgtc tttccctcct aaaatagctg gttgttgttt atggaattct
    33541 gttgcttgtt caaacgcata tgccgcttga tacatcgttt tttcatcaaa ataattaccg
    33601 atgatctgca atccaactgg taatccttct gataaacctg ccggtacaga catacctgga
    33661 agtccagcca agttgacagg gatcgttaag atgtcactca tgtacattgt gattggatcg
    33721 ttaatgttct caccgattcc gaatgcaact gttggtgaag atggcccgat gatcaagtca
    33781 taatctgcaa agactttttc aaaatctcgt ttgatcaatg tacggacttg tcctgctttt
    33841 ttgaagtaag cgtcataata acccgcgctt aaggagaatg tacctaacat gatacggcgt
    33901 tttacttctt caccgaatcc ttctgaacgt gagttgacat atacatcatc taatgatttc
    33961 acattttcag agcgataacc gtagcggata ccatcgaatc gttgcaaatt ggaacttgct
    34021 tctgatgagg caatgatata ataaacagcc acaccgtatt ttgaatgagg caagctgact
    34081 tcttccacaa cagcacctaa tgaacggaat gtttctgctg ctttcaatac agcttctttt
    34141 acgccagggg ccacaccttc gcctaagtat tctttaggta aagcaatctt cattcctttg
    34201 atgtcgcctg tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga agtaccgtct
    34261 ttttcgtcat aaccgctgat agctgtcaat gctaatgcat tatctttcac cgtacgggtc
    34321 attggtccga tttggtcaag agatgaagca aaggcaatca aaccaaaacg tgagacacgg
    34381 ccataagtag gtttcatccc tacgatgccg ttaaaagctg ctggttggcg gatacttccc
    34441 ccagtatcac tacctaaaga aactggaatt tgtccagctg caactgctgc tgcagaacca
    34501 ccagaagagc caccaggtac ttttgttggg tcccaagcat tttttgtttt cttgaaataa
    34561 gaagtttctg tacttccacc catagcaaat tcatccatgt ttagcttacc aacagggatc
    34621 atatctgctt gatagatttt atccatgacc gttgcatcat aaattggttc aaaattgtaa
    34681 aggatcttag atgcagcggt tgtcaagata tcttttgtta cgatattatc cttgatacca
    34741 attggaatac cagcaaggac gttgctttca tcaatccctt tttcatctaa agcttttgcc
    34801 tgttccaatg ctttttcttc gttcaatgta atgaatgcct cgacctcagg ctctgtttct
    34861 ttgattcgtt caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt cgatacaagc
    34921 atatgatgca gttcttctag agaacgattg tgtaattctg tcattatgcg ccagcctccc
    34981 cattatcgat gattgcgggt actttgataa agccgtcttc tgtttccggc acatttttca
    35041 ataattcatc gcggttccat ccagcttgcg cccgatcttg tcgcatcacg tttactgttt
    35101 caaccacatt cgacgtaaat ggaacacctt ccgtatctac ttcttccagc atttcaacca
    35161 tatcgatgat tttacctaat tggtcggtaa aacttgcaag ttcttcatct gcgaacgcaa
    35221 gttttgctaa tttggcaacg tgtttcactt gttcttcact aatcgccata ctaccccttc
    35281 tttctaaacg agttaaaaac tttgctctct attttaacac aagagtgccg tgtctgaaat
    35341 tgtttattga aatttgtctg aaaaaactca tttttcattc ttctactgag cgacttttag
    35401 ccaatgcatc tgccatttga tcttctgtcc aaacttcgat tcctaattct tgtgctttgg
    35461 tcagcttgct gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac
    35521 cagtcacttt cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg
    35581 ttagtttacc agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt
    35641 gggcatttcg aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca
    35701 attcatggac ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga
    35761 tcgtatcaag cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga
    35821 aatgttctgc taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa
    35881 tcaaccgctc gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg
    35941 atttttcttt tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag
    36001 caacatctgc tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat
    36061 cgatattcat cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc
    36121 atttaggatt gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac
    36181 aaactggaca atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca
    36241 cgacttgcga aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca
    36301 agcggatatc cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg
    36361 ttcctgctac tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga
    36421 ctgtccattc gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg
    36481 ctgcccatct cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca
    36541 ctttgatgac gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt
    36601 caatgtatga ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga
    36661 atccaatttt ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga
    36721 aatcagctac tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta
    36781 actgacgcaa agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg
    36841 cttctctgtc ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta
    36901 cttcgatgtt cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt
    36961 cagtgatgtt ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat
    37021 cttcataacg caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta
    37081 cacgtcccac tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa
    37141 cgtcgttcaa actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt
    37201 ttccgcctac tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga
    37261 ttgctactaa ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt
    37321 ctacgtagta ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag
    37381 cagcttcgtc aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca
    37441 attggcgcaa aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt
    37501 tctagatcct tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt
    37561 ttgactttat ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga
    37621 acaaccgctg tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt
    37681 tttttaggcg taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca
    37741 aaacgagaag gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt
    37801 aaatacaacg cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc
    37861 agttcgcttt cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc
    37921 aggaatacaa ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg
    37981 gaagcatctt cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc
    38041 gttagttttt cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg
    38101 aattcttgtg tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat
    38161 gtattttgga tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat
    38221 tctgtcactg tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca
    38281 cctaattgct gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca
    38341 agcagtggcc attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg
    38401 atgcctcgtt tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc
    38461 agcacattca aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct
    38521 ccaaccattt tataaggaat atttgctttc aacagagttt cttccattac acgagattgt
    38581 gcgttcgtcc ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct
    38641 tcttgcatcc ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa
    38701 gtgatcttgt ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg
    38761 ttttcaatga cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct
    38821 agcaaaatta ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct
    38881 gcaccgcgcc agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat
    38941 ctacctgcca ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct
    39001 acatgaatat aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat
    39061 aatcgaatcg tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct
    39121 ttttggtaag cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt
    39181 gcaaaatcct gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt
    39241 cgaggatcat attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc
    39301 agctgttcag aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct
    39361 cttcttaaaa tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca
    39421 gatgcgagaa tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta
    39481 atcgctagga tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt
    39541 gtcagcactc tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg
    39601 tgaagaacag cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac
    39661 attaacaaca tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt
    39721 aaactagcta gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg
    39781 atttcgcgct aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag
    39841 aaattagata agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca
    39901 ggaatcgtcg ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt
    39961 atctatgaaa tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc
    40021 cttgcagcat ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg
    40081 attccacaag tagagggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta
    40141 tggaaaaagt tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa
    40201 ggaccttcta tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt
    40261 ccatcttcag aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca
    40321 ttcaatgctc ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct
    40381 ccacttgttt ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac
    40441 tttttcggct tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat
    40501 tatggttcct tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat
    40561 gtcttgctgg ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg
    40621 ttcgttcctt tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga
    40681 ggcaatcaaa tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt
    40741 ttgtttgttc ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg
    40801 attttccttc cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg
    40861 gttgatttcg ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt
    40921 tatttcacag cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt
    40981 ggctctctta atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat
    41041 gaccttctcg gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact
    41101 cagaccgcct cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt
    41161 tctttagacg gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct
    41221 atacgcagag gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat
    41281 attttggtta tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct
    41341 aaatcttcta tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca
    41401 acaactattc atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg
    41461 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
    41521 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
    41581 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
    41641 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
    41701 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
    41761 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
    41821 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
    41881 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
    41941 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
    42001 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
    42061 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
    42121 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
    42181 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
    42241 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
    42301 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
    42361 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
    42421 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
    42481 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
    42541 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
    42601 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
    42661 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
    42721 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
    42781 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
    42841 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
    42901 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
    42961 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
    43021 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
    43081 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
    43141 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
    43201 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
    43261 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
    43321 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
    43381 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
    43441 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
    43501 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
    43561 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
    43621 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
    43681 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
    43741 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
    43801 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
    43861 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
    43921 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
    43981 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
    44041 agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt
    44101 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
    44161 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
    44221 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
    44281 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
    44341 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
    44401 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
    44461 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
    44521 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
    44581 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
    44641 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
    44701 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
    44761 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
    44821 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
    44881 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
    44941 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
    45001 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
    45061 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
    45121 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
    45181 tataaatcag tcaaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt
    45241 gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac
    45301 agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat
    45361 gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga
    45421 taataatgat gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc
    45481 atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca
    45541 gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt
    45601 tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt
    45661 ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca tctgttacct
    45721 ctacttctag atattcccta atataataac aataagcttc gattccatta tccaaagcaa
    45781 cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa
    45841 aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga
    45901 tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacgggt tgcatttgtg
    45961 cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg
    46021 gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa
    46081 aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct
    46141 ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa
    46201 aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc caacacgaag
    46261 tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta
    46321 cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca
    46381 atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat
    46441 aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct
    46501 tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc
    46561 gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg
    46621 ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag
    46681 agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct
    46741 gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
    46801 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
    46861 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
    46921 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
    46981 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
    47041 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
    47101 taagctc
//
LOCUS       DACEMK010000007        45192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEMK010000007 DACEMK010000000
VERSION     DACEMK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.007"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..2095)
                     /locus_tag="IW715_000343"
     CDS             complement(23..2095)
                     /locus_tag="IW715_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1020971.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IW715_000344"
     CDS             complement(2028..2405)
                     /locus_tag="IW715_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1020972.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IW715_000345"
     CDS             complement(2534..3094)
                     /locus_tag="IW715_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1020973.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IW715_000346"
     CDS             3244..4536
                     /locus_tag="IW715_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1020974.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IW715_000347"
     CDS             complement(4596..5714)
                     /locus_tag="IW715_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1020975.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW715_000348"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IW715_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1020976.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW715_000349"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IW715_000349"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1020977.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IW715_000350"
     CDS             8920..9519
                     /locus_tag="IW715_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1020978.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW715_000351"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IW715_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1020979.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IW715_000352"
     CDS             complement(11214..11558)
                     /locus_tag="IW715_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1020980.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IW715_000353"
     CDS             complement(11695..12831)
                     /locus_tag="IW715_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1020981.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IW715_000354"
     CDS             complement(13020..13994)
                     /locus_tag="IW715_000354"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1020982.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IW715_000355"
     CDS             complement(14074..14949)
                     /locus_tag="IW715_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1020983.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IW715_000356"
     CDS             15079..16986
                     /locus_tag="IW715_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1020984.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IW715_000357"
     CDS             complement(17527..20805)
                     /locus_tag="IW715_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1020985.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IW715_000358"
     CDS             complement(21076..21621)
                     /locus_tag="IW715_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1020986.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IW715_000359"
     CDS             complement(21633..22325)
                     /locus_tag="IW715_000359"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1020987.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IW715_000360"
     CDS             complement(22344..22628)
                     /locus_tag="IW715_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1020988.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IW715_000361"
     CDS             complement(22641..23204)
                     /locus_tag="IW715_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1020989.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IW715_000362"
     CDS             23432..24094
                     /locus_tag="IW715_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1020990.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IW715_000363"
     CDS             24218..25414
                     /locus_tag="IW715_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1020991.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIAFTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IW715_000364"
     CDS             complement(25515..26891)
                     /locus_tag="IW715_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1020992.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IW715_000365"
     CDS             complement(26928..27794)
                     /locus_tag="IW715_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1020993.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IW715_000366"
     CDS             complement(27883..28908)
                     /locus_tag="IW715_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1020994.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IW715_000367"
     CDS             complement(28926..29600)
                     /locus_tag="IW715_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1020995.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IW715_000368"
     CDS             complement(29784..30677)
                     /locus_tag="IW715_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1020996.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IW715_000369"
     CDS             complement(30751..32448)
                     /locus_tag="IW715_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1020997.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IW715_000370"
     CDS             complement(32441..33754)
                     /locus_tag="IW715_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1020998.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37272)
                     /locus_tag="IW715_000371"
     CDS             complement(33754..37272)
                     /locus_tag="IW715_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1020999.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAISDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37565..38023)
                     /locus_tag="IW715_000372"
     CDS             complement(37565..38023)
                     /locus_tag="IW715_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1021000.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38321..38839)
                     /locus_tag="IW715_000373"
     CDS             complement(38321..38839)
                     /locus_tag="IW715_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1021001.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(38841..39458)
                     /locus_tag="IW715_000374"
     CDS             complement(38841..39458)
                     /locus_tag="IW715_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1021002.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39455..40309)
                     /locus_tag="IW715_000375"
     CDS             complement(39455..40309)
                     /locus_tag="IW715_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1021003.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40306..41217)
                     /locus_tag="IW715_000376"
     CDS             complement(40306..41217)
                     /locus_tag="IW715_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1021004.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41258..42592)
                     /locus_tag="IW715_000377"
     CDS             complement(41258..42592)
                     /locus_tag="IW715_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021005.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            42800..43630
                     /locus_tag="IW715_000378"
     CDS             42800..43630
                     /locus_tag="IW715_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1021006.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(43874..44123)
                     /locus_tag="IW715_000379"
                     /pseudo
     CDS             complement(43874..44123)
                     /locus_tag="IW715_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44314..44508)
                     /locus_tag="IW715_000380"
     CDS             complement(44314..44508)
                     /locus_tag="IW715_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021007.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        13071 a         9721 c         7969 g        14431 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgtggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg ttaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta tcattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttccttttct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc atttaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ttgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    37561 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    37621 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    37681 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    37741 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    37801 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    37861 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    37921 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    37981 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    38041 aaaaatattt gtcttctttc acaacaatca acaaaacaat gcccatctaa ttataattta
    38101 atttaatttt aattttaaat caaatgatat cctttttagt taaaaaacac caattataca
    38161 acatttatta ttaaaattaa attataaaat tttataacgt tctcatttaa caaaacttaa
    38221 gttattaaga caaatgaata aacaactacg ctttcctcga aacaaaaaaa taaagcacaa
    38281 atgcgcattt acgcactcat gctttattcg tacaattaat tcagataacg gcagcgcata
    38341 ggatgccaat aacacttcct aaaatagctc ctcctaaaat gtcagtagga taatgtaaaa
    38401 gtaaaaaaat cctcgaataa gcgatcaaac aagcaaataa aaaggctaat attcctgcaa
    38461 ctggacttaa ttcgaacaac gcagctactg ataaaaaggc tagcgctgta tgtcctgaag
    38521 gaaaagaaga agtcttcggt ttacttgtgt aaaggacaag ccgacgattt ttttcataag
    38581 gtcttttccg tcttgtcgtt tttttgataa aaagcaaacc aacaaaaaaa tagatcaaac
    38641 tgccgataag aatggctaga ccatattctc tagtatcagg tatcaagact aaaacagcag
    38701 caaggagcaa ccaaataaaa ccaccattag agatattact gataaatttc aaaaaattga
    38761 tttttcgact atccgttttc cgttgcttgg cgaacttgtt gataatacta tcatcgattt
    38821 tagcaatcaa acgcaacatg ttattcctcc tttttaagaa ccttttcttg cgtacgatgg
    38881 ataaaaagca ggacttgaaa ataaaacaag aaactgaaac ctaataaagc aaaatagatt
    38941 ggtcctgcaa accagaatgc gacaagaagg atcaacgtgc ctgccaaata gaaaagcgcg
    39001 cttttgaaat cattaaaaaa caagaaaaat gcagtaatcc aagaagcttc tttttgttcc
    39061 agttgaagca agattttgta actaaagaga agcgcgctta atagaagcaa aaacaacgta
    39121 gtatacatca atgtatagcc aattccccca ccccaatagc ggaaaatctg atagtcgatc
    39181 aatagaagaa gaaaataaac acttgctaaa ctatcttgct ttccataaag agagaagttc
    39241 tcacgaaact ttttccagaa tagtgaaccg agctttttct cttcattttt tttgtaatga
    39301 taaatcatat acagaactgt tcgacttgca gttcctaatg tgaaaacagg caagacgcac
    39361 aggaaccata gtagctgtaa atggataaga cgtggaatcc attcaatgat ttctaagaaa
    39421 taatcattgg cttttttacc aatcgatcga ttcatcatcc ttttgaacct cctgtcaaat
    39481 ccatcccttc aatgaaataa cgttgtgcaa aaaagaacag caaaatcgtc gggatcaaga
    39541 caagtgttgc tcctgccatc agataattcc attgtgtcgt tgcttggttc tgaaaaactt
    39601 gcaagccaat ctgtaacgtg tatttttctt gatcttggat atataacaga ggacctaaga
    39661 aatcattcca agctgcgttg aaggaattga tcgcaatggt aatcaaggca ggtttcgtca
    39721 atggaagcat caaatgactc caaatataca aatgattcgc tccgtcgatt tcagcagctt
    39781 cgatcaattc attattgatc gataagtaaa attgacgcat cagaaatata ttgaacgcat
    39841 tgccgaagaa cgatggaacg atcaggggca aataagtacc tacccagccg atcttggaga
    39901 ataatacata ttgagggatc atcgttacaa accccgggat catcatcgta gaaagaacca
    39961 aagcaaacat cagctttttc ccaggaaaat caagcttcgc aaatccataa gcaataaaag
    40021 aatttgaaag gacattaccg atcactacta gcacagtgat gaataatgta tttcttgcat
    40081 agcgccaaaa aggaaaggcg gcaatcgtat ccagatagtt ttcccatacg attttttctg
    40141 ggaaaaaggt cggaggataa cgaacgattt cttccattgg tttcaatgaa gtaaacacca
    40201 tccaaagcaa tagaatgatc aaaatgattc ctattactgt catgaggaca taacttagta
    40261 ttttttttgt ttgccgattc acactggtct ttttgaaata atccatcatt tgccgtcacc
    40321 tccagaataa tagacccact taggtgcaag cttcaaattg acaaacgtca gaaccaacac
    40381 gatcagaaac aggatcattg acatagccat cgcataaccc atatcaaaag ctacgaacgc
    40441 cttgttccac atatgcagat tataaaaaag caaagaattt gcaggtccgc ctgttccatt
    40501 tttcgtcatg acataagctt cttgaaaaat ttggaaagaa ccgatcgtag aggtgatcac
    40561 atcgtaaaaa ataataggag tgattaatgg caaagtaata cttttgaact ttccccaaac
    40621 gcctgctcca tcgatttctg ctgattcata taaagatcta ggaacatttt gtaatgtagc
    40681 taagtaaagt agcattgctc ctccagaact ccatattttc atcaaaatca acgcaggttt
    40741 cgtccattgt ggatcaaaca gccaagctgg tccagggata tggaaccagc ccagaaatgt
    40801 gttgatcaat cctgcagatg gagaaagcag ctgcatccat aagatgtaga ctgctactcc
    40861 tgataagact gccggtaaat agaaagctgt acggaataca ctgattcctt tgattttttg
    40921 attgagtact gtggctaaaa acacactgaa aatcgtcgtc agcggtacat tgaagatgac
    40981 gaaataaacg gtgttgtata acgatttcca aaataaatca tcgtgaacga acattctttt
    41041 gaaatttgct agaccaatga aattcatctt ggatgtcaaa ttataatcgg tgaaactacc
    41101 aataactgaa aaaagcatag gccctagcat aaataccaag aacccgataa taaaaggcga
    41161 gataaataaa taggcccatc ttgcttcttt tttctttcgg atagataaac gcttcaaatt
    41221 ttcttgcctc ctctgtacct agacagtcaa aaatggatta ttgattttgc ttgactaagt
    41281 tttctactgc tttttgtgca tctgccaatc cttcttttgg tgattttgtt cctaaaagaa
    41341 tctggtcaat ctgttcattc actaatgtcg tgtaatccgg agcatctaaa ggaactggtg
    41401 tgatcaccgt atttttaaag ttttcgtccg ccatttgata gatcattttt cctttatccg
    41461 ttaggttgtc attattcact aaattctcat tggctgtctt gctcgccatg atatcaaaac
    41521 ttttttcgcc gaatttttct tgtacttctg gagaagaaag atatttgatg aattcataag
    41581 aggcctcagg atcttttgct ccgtatggta cttccaagac gaatccgcca ccccacgacc
    41641 aatggcctga accttcttct ttttctggga gctgcatcac gccaaaatca aaatcatccg
    41701 gtgcattttc catcaaacta gagtaataat tgatattttg ggcacgcatg gctactaatc
    41761 cggaaataaa tggatcagat acaccagacc caaattcggc ttctaattta ttgatcgtat
    41821 ttttcccata ataatcttgc cagtcttgga tccactccaa ggcttccact ttatttttag
    41881 tatcaatctt gactttttca tctttgtcaa accaactagt cccgttatct gcgtttagcg
    41941 cccatacatc agcccctaaa ttccataatg gataaaaacc gatgcgtgta aattcttttc
    42001 cttctttttt atctagttta cgtgctgttt cttctaattc atcccaagtt gtcggtagat
    42061 cttgttcact gattccagct tctttaaata actttttatt ataaaaaatg acttgtgtat
    42121 ctgtattgaa tgggatcgca taaggatcac cttcatattc tactgtatcc caaagctgcg
    42181 gataaaaatc cttagaagtg tctttttcta catatttgga gagattagtt gcttgctgtg
    42241 cttctgctct ttgggcaacg gaatttatgt cctgtacaat aacatcgggt gcatttccag
    42301 ctgttattgc agataaagat ttagtccaga tgtcacccca tggctgatac ttataattta
    42361 catggatctc gttttgtgat tgattgaaat cctcaatgat ttcttcgata gcttcttttc
    42421 gtgcaccgga tccccaaaag gaccagaaat caatattttt tcttccatca cttgtttttt
    42481 cagaagaatt tgctgcactt gaactggcat cgcttgcatt acttgatccg catcctccta
    42541 ataaaaacaa tgctgaactt gccagcaacc ctgtaaacaa taattttttc atattttacc
    42601 tccaaatttt aatgtaaccg atgccggttt taatataacg atatcatttt gttctgaaaa
    42661 taacaattta taaggtatga ttctaaaaaa attattaatg ataccaatac tttatataat
    42721 tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca gaatgagaat gtattaaaaa
    42781 tagattagga ggaattttga tgtttagaca ccagaaagaa ttgcagtttg aagcaaaacc
    42841 agatcgtccg aatccattaa tcgcgaaata tctgcaggag ctgatcggcg gccaatatgg
    42901 cgagatgagt gtagctatgc agtacctgtt ccaaggctgg agctgccgag gagaagaaaa
    42961 atataaagat atgctgatgg acattgcaac agaagaaatc ggccacgtgg aaatgttggc
    43021 gacaatggtt gctcgtttat tagagggtgc gcctgttgaa gatcaagaag cagcgatgca
    43081 aaaagatcca acaatcggtg ccattatctc tggaatgaat ccacagcacg cgatcgtagc
    43141 tggcttagga cctcgtcctg ctgacagcgt gggcaatcca tggagcggtg cctatatcat
    43201 tgcgagtggc aacttgttag cagactttag agctaattta aatgccgaat cacaaggaag
    43261 acttcaagta gcacgtattt acgaaatgat cgatgatcgt ggcgtaaaag atatgctgtc
    43321 tgttctcctt gcaagagata gctatcatca aaatctatgg gaatgtgcag tgaaagaatt
    43381 agaagaaaaa gaaggttcaa tcttagtccc aagtactttc ccgcgtgaca aagaacgaaa
    43441 agacattgct tatgacttct ataacttctc agagggagac caaagtagtg aaggttcttg
    43501 ggctagtggc aaagcactgg atggcgaagg cgaatatcaa tggttgaaag aacctaagat
    43561 cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa atgtatggga cacctccatt
    43621 caagaaataa atatctaccc gtagaaacac ccgttttttg ttccacacgc tttgtgagca
    43681 aaactgacta aagtcattaa tcataaccac ccttcaaaag ggtggttttc tctagagcta
    43741 taagctcaaa tcaccggcca gcgcctaaag acgctggctt tcatatttta tgttcaagcc
    43801 atattgcctt tgccagtttt gcccacctct taaaaggttt atgtactact ccttagccct
    43861 taaaaggatc gtcttactct cgtacactca gcttatctaa cgcactatca tgtttttctt
    43921 gttcttgaat gtactttttg atggtcgctt cattttgtcc tacttttgtc acatagtatc
    43981 cttccaccca aaagtgtcga tacccaaatt tatattttag atttgcacaa tctcttgatg
    44041 atctcctgaa tattggctcg atattgatta taaattattt ttcttctata ctttggtgtg
    44101 aatacaatgt ggtatttaca catctacttt gtacgggcta aactatttcc ctttattgct
    44161 ccacccgcat agcaagtggt tttgtatttc aatcgctcgg cgcgctcaat taggtctaaa
    44221 gacaataaat ctaaaaaccc gctgcttcac tgacagaaac aacgggtttt cagaaaaaaa
    44281 tgaaatgaaa aagatctctt acggcaatct attttatttt tttgattttt tataaatgga
    44341 aaatgcttca agaagtccaa gaaataaagc cgtataaaaa caagaaccta tgaaatcttt
    44401 atttattaga tattcaacag aaatcccgat aacactagcc aacaatatat ttatcagaag
    44461 tgtaaaataa ggatgctttt tatagaatag aactagtgtt gttttcatag actctccttt
    44521 cttcatattt ccaattaatc acaaattcac ggcattacta gaaaaattaa aggggcgcag
    44581 aagtggaaaa atactcttcg actttcccta caaaccaggc catcttgtgt ttaataacta
    44641 taccaaatct aaggtacctc taaatctaaa ttccatttgt ggcggtgacc tccaattttt
    44701 aactcttcta tctcccatcc agtatacata accttgatac ttcgaccact tatatatcga
    44761 acacacaaat acctatccat tgcaggagta ctacctaact tagttgctac ttgcatctaa
    44821 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
    44881 ctatcatttt tatcctcaac tacatgcaca tctgttgcaa cttcactttc tgtctcatcc
    44941 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
    45001 ctaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
    45061 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
    45121 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
    45181 tttagaattt tt
//
LOCUS       DACEMK010000008        44615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEMK010000008 DACEMK010000000
VERSION     DACEMK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.008"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(218..1015)
                     /locus_tag="IW715_000381"
     CDS             complement(218..1015)
                     /locus_tag="IW715_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1021008.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IW715_000382"
     CDS             complement(1012..2196)
                     /locus_tag="IW715_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1021009.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IW715_000383"
     CDS             complement(2221..3468)
                     /locus_tag="IW715_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1021010.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IW715_000384"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IW715_000384"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1021011.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IW715_000385"
     CDS             5448..5762
                     /locus_tag="IW715_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1021012.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IW715_000386"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IW715_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1021013.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IW715_000387"
     CDS             6447..7091
                     /locus_tag="IW715_000387"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1021014.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IW715_000388"
     CDS             7106..7435
                     /locus_tag="IW715_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021015.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IW715_000389"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IW715_000389"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1021016.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IW715_000390"
     CDS             8423..9247
                     /locus_tag="IW715_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1021017.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IW715_000391"
     CDS             9240..9587
                     /locus_tag="IW715_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1021018.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IW715_000392"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IW715_000392"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1021019.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IW715_000393"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IW715_000393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1021020.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IW715_000394"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IW715_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1021021.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IW715_000395"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IW715_000395"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1021022.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IW715_000396"
     CDS             15244..16011
                     /locus_tag="IW715_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021023.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IW715_000397"
     CDS             16001..18043
                     /locus_tag="IW715_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021024.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IW715_000398"
     CDS             18324..18473
                     /locus_tag="IW715_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1021025.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IW715_000399"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IW715_000399"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1021026.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IW715_000400"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IW715_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1021027.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IW715_000401"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IW715_000401"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1021028.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IW715_000402"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IW715_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1021029.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IW715_000403"
     CDS             22890..23972
                     /locus_tag="IW715_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1021030.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IW715_000404"
     CDS             23972..24775
                     /locus_tag="IW715_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1021031.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGVNLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IW715_000405"
     CDS             24845..25585
                     /locus_tag="IW715_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1021032.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IW715_000406"
     CDS             25596..26600
                     /locus_tag="IW715_000406"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1021033.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IW715_000407"
     CDS             complement(26645..28489)
                     /locus_tag="IW715_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021034.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28701..29219
                     /locus_tag="IW715_000408"
     CDS             28701..29219
                     /locus_tag="IW715_000408"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1021035.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29347..30030
                     /locus_tag="IW715_000409"
     CDS             29347..30030
                     /locus_tag="IW715_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1021036.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30339..30412
                     /locus_tag="IW715_000410"
     tRNA            30339..30412
                     /locus_tag="IW715_000410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30373..30375,aa:Arg,seq:tct)
     gene            30571..30930
                     /locus_tag="IW715_000411"
     CDS             30571..30930
                     /locus_tag="IW715_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1021037.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31053..32684
                     /locus_tag="IW715_000412"
     CDS             31053..32684
                     /locus_tag="IW715_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1021038.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32746..33387)
                     /locus_tag="IW715_000413"
     CDS             complement(32746..33387)
                     /locus_tag="IW715_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1021039.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33669..33977
                     /gene="rplU"
                     /locus_tag="IW715_000414"
     CDS             33669..33977
                     /gene="rplU"
                     /locus_tag="IW715_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1021040.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34004..34348
                     /locus_tag="IW715_000415"
     CDS             34004..34348
                     /locus_tag="IW715_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1021041.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34361..34654
                     /gene="rpmA"
                     /locus_tag="IW715_000416"
     CDS             34361..34654
                     /gene="rpmA"
                     /locus_tag="IW715_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1021042.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35061..37895
                     /locus_tag="IW715_000417"
     CDS             35061..37895
                     /locus_tag="IW715_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1021043.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38086..38577
                     /locus_tag="IW715_000418"
     CDS             38086..38577
                     /locus_tag="IW715_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1021044.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38680..39669
                     /gene="manX"
                     /locus_tag="IW715_000419"
     CDS             38680..39669
                     /gene="manX"
                     /locus_tag="IW715_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1021045.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39702..40505
                     /locus_tag="IW715_000420"
     CDS             39702..40505
                     /locus_tag="IW715_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1021046.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40526..41440
                     /locus_tag="IW715_000421"
     CDS             40526..41440
                     /locus_tag="IW715_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1021047.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41523..41897
                     /locus_tag="IW715_000422"
     CDS             41523..41897
                     /locus_tag="IW715_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1021048.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42036..42281
                     /locus_tag="IW715_000423"
     CDS             42036..42281
                     /locus_tag="IW715_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1021049.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42453..43517
                     /locus_tag="IW715_000424"
     CDS             42453..43517
                     /locus_tag="IW715_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1021050.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43763..44188
                     /locus_tag="IW715_000425"
     CDS             43763..44188
                     /locus_tag="IW715_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1021051.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44181..>44615
                     /gene="nusB"
                     /locus_tag="IW715_000426"
     CDS             44181..>44615
                     /gene="nusB"
                     /locus_tag="IW715_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1021052.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT        14170 a         7688 c         9366 g        13391 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcgtcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    28681 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    28741 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    28801 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    28861 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    28921 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    28981 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    29041 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    29101 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    29161 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    29221 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    29281 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    29341 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    29401 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    29461 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    29521 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    29581 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    29641 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    29701 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    29761 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    29821 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    29881 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    29941 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    30001 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    30061 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    30121 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    30181 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    30241 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    30301 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    30361 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    30421 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    30481 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    30541 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    30601 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    30661 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    30721 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    30781 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    30841 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    30901 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    30961 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    31021 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    31081 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    31141 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    31201 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    31261 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    31321 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    31381 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    31441 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    31501 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    31561 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    31621 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    31681 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    31741 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    31801 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    31861 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    31921 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    31981 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    32041 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    32101 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    32161 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    32221 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    32281 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    32341 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    32401 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    32461 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    32521 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    32581 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    32641 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    32701 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    32761 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    32821 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    32881 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    32941 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    33001 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    33061 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    33121 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    33181 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    33241 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    33301 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    33361 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    33421 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    33481 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    33541 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    33601 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    33661 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    33721 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    33781 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    33841 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    33901 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    33961 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    34021 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    34081 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    34141 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    34201 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    34261 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    34321 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    34381 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    34441 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    34501 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    34561 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    34621 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    34681 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    34741 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    34801 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    34861 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    34921 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    34981 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    35041 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    35101 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    35161 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    35221 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    35281 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    35341 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    35401 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    35461 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    35521 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    35581 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    35641 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    35701 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    35761 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    35821 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    35881 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    35941 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    36001 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    36061 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    36121 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    36181 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    36241 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    36301 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    36361 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    36421 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    36481 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    36541 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    36601 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    36661 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    36721 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    36781 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    36841 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    36901 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    36961 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    37021 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    37081 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    37141 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    37201 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    37261 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    37321 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    37381 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    37441 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    37501 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    37561 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    37621 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    37681 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    37741 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    37801 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    37861 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    37921 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    37981 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    38041 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    38101 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    38161 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    38221 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    38281 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    38341 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    38401 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    38461 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    38521 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    38581 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    38641 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    38701 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    38761 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    38821 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    38881 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    38941 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    39001 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    39061 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    39121 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    39181 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    39241 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    39301 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    39361 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    39421 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    39481 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    39541 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    39601 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    39661 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    39721 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    39781 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    39841 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    39901 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    39961 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    40021 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    40081 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    40141 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    40201 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    40261 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    40321 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    40381 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    40441 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    40501 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    40561 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    40621 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    40681 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    40741 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    40801 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    40861 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    40921 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    40981 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    41041 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    41101 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    41161 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    41221 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    41281 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    41341 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    41401 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    41461 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    41521 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    41581 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    41641 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    41701 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    41761 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    41821 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    41881 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    41941 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    42001 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    42061 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    42121 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    42181 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    42241 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    42301 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    42361 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    42421 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    42481 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    42541 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    42601 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    42661 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    42721 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    42781 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    42841 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    42901 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    42961 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    43021 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    43081 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    43141 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    43201 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    43261 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    43321 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    43381 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    43441 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    43501 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    43561 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    43621 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    43681 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    43741 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    43801 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    43861 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    43921 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    43981 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    44041 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    44101 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    44161 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    44221 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    44281 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    44341 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    44401 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    44461 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    44521 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    44581 tcaacggtgt tttatccaat gtattgaaag aatta
//
LOCUS       DACEMK010000009        43154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEMK010000009 DACEMK010000000
VERSION     DACEMK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.009"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            117..971
                     /locus_tag="IW715_000427"
     CDS             117..971
                     /locus_tag="IW715_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1021053.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="IW715_000428"
     CDS             972..2309
                     /locus_tag="IW715_000428"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1021054.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="IW715_000429"
     CDS             2314..2538
                     /locus_tag="IW715_000429"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1021055.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="IW715_000430"
     CDS             2540..3430
                     /locus_tag="IW715_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1021056.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="IW715_000431"
     CDS             3439..4257
                     /locus_tag="IW715_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1021057.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="IW715_000432"
     CDS             4364..4813
                     /locus_tag="IW715_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1021058.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="IW715_000433"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="IW715_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1021059.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="IW715_000434"
     CDS             complement(6568..6684)
                     /locus_tag="IW715_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1021060.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="IW715_000435"
     CDS             complement(6757..7713)
                     /locus_tag="IW715_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1021061.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="IW715_000436"
     CDS             7884..8252
                     /locus_tag="IW715_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1021062.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="IW715_000437"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="IW715_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1021063.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="IW715_000438"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="IW715_000438"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1021064.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="IW715_000439"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="IW715_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1021065.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="IW715_000440"
     CDS             10240..12432
                     /locus_tag="IW715_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1021066.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="IW715_000441"
     CDS             12458..13420
                     /locus_tag="IW715_000441"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1021067.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="IW715_000442"
     CDS             13434..14795
                     /locus_tag="IW715_000442"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1021068.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="IW715_000443"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="IW715_000443"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1021069.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="IW715_000444"
     CDS             15924..17144
                     /locus_tag="IW715_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1021070.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="IW715_000445"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="IW715_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1021071.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW715_000446"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="IW715_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1021072.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="IW715_000447"
     CDS             19906..20583
                     /locus_tag="IW715_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1021073.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="IW715_000448"
     CDS             20597..21196
                     /locus_tag="IW715_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1021074.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="IW715_000449"
     CDS             21211..21498
                     /locus_tag="IW715_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1021075.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="IW715_000450"
     CDS             21547..22329
                     /locus_tag="IW715_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1021076.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="IW715_000451"
     CDS             22350..23057
                     /locus_tag="IW715_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1021077.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="IW715_000452"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="IW715_000452"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1021078.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="IW715_000453"
     CDS             complement(26166..27692)
                     /locus_tag="IW715_000453"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1021079.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="IW715_000454"
     CDS             complement(27707..28354)
                     /locus_tag="IW715_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1021080.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="IW715_000455"
     CDS             complement(28538..28867)
                     /locus_tag="IW715_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1021081.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="IW715_000456"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="IW715_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1021082.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="IW715_000457"
     CDS             29788..30801
                     /locus_tag="IW715_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1021083.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="IW715_000458"
     CDS             30801..32078
                     /locus_tag="IW715_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1021084.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="IW715_000459"
     CDS             32141..34843
                     /locus_tag="IW715_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1021085.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="IW715_000460"
     CDS             34995..35312
                     /locus_tag="IW715_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1021086.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="IW715_000461"
     CDS             35342..35662
                     /locus_tag="IW715_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1021087.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37208
                     /locus_tag="IW715_000462"
     CDS             35748..37208
                     /locus_tag="IW715_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1021088.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37276..37497
                     /locus_tag="IW715_000463"
     CDS             37276..37497
                     /locus_tag="IW715_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021089.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37528..37710
                     /locus_tag="IW715_000464"
     CDS             37528..37710
                     /locus_tag="IW715_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1021090.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37710..38123
                     /locus_tag="IW715_000465"
     CDS             37710..38123
                     /locus_tag="IW715_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1021091.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38246..39427
                     /locus_tag="IW715_000466"
     CDS             38246..39427
                     /locus_tag="IW715_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1021092.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39816..39889
                     /locus_tag="IW715_000467"
     tRNA            39816..39889
                     /locus_tag="IW715_000467"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39850..39852,aa:Arg,seq:cct)
     gene            complement(39958..41097)
                     /locus_tag="IW715_000468"
     CDS             complement(39958..41097)
                     /locus_tag="IW715_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1021093.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41396..42031)
                     /locus_tag="IW715_000469"
     CDS             complement(41396..42031)
                     /locus_tag="IW715_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021094.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42144..42779)
                     /locus_tag="IW715_000470"
     CDS             complement(42144..42779)
                     /locus_tag="IW715_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1021095.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42813..>43154)
                     /locus_tag="IW715_000471"
     CDS             complement(42813..>43154)
                     /locus_tag="IW715_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021096.1"
                     /translation="KTIPFKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDE
                     NTVMFTKKYWNEMENLKKYIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEE
                     FDFKKKELLGL"
BASE COUNT        14278 a         7349 c         9047 g        12480 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    36001 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    36061 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    36121 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    36181 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    36241 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    36301 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    36361 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    36421 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    36481 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    36541 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    36601 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    36661 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    36721 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    36781 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    36841 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    36901 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    36961 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    37021 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    37081 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    37141 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    37201 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    37261 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    37321 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    37381 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    37441 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    37501 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    37561 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    37621 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    37681 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    37741 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    37801 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    37861 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    37921 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    37981 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    38041 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    38101 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    38161 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    38221 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    38281 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    38341 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    38401 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    38461 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    38521 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    38581 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    38641 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    38701 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    38761 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    38821 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    38881 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    38941 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    39001 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    39061 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    39121 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    39181 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    39241 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    39301 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    39361 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    39421 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    39481 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    39541 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    39601 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    39661 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    39721 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    39781 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    39841 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    39901 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    39961 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    40021 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    40081 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    40141 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    40201 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    40261 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    40321 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    40381 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    40441 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    40501 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    40561 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    40621 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    40681 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    40741 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    40801 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    40861 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    40921 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    40981 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    41041 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    41101 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    41161 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    41221 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    41281 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    41341 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    41401 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    41461 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    41521 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    41581 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    41641 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa
    41701 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    41761 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    41821 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    41881 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    41941 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    42001 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    42061 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    42121 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    42181 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    42241 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    42301 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    42361 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    42421 ctaatatatt tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta
    42481 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    42541 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    42601 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    42661 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    42721 aacatgatta aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt
    42781 aatagatcct ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta
    42841 aaatcgaatt cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt
    42901 tcatctgcac ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc
    42961 tctatatatt tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg
    43021 ttttcatctt tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta
    43081 aattgtatat aaccattact catacctggt ttttttaatt gtacggctga tatattttta
    43141 aaggggatag tttt
//
LOCUS       DACEMK010000010        41449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEMK010000010 DACEMK010000000
VERSION     DACEMK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.010"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..828)
                     /locus_tag="IW715_000472"
     CDS             complement(<1..828)
                     /locus_tag="IW715_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1021097.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFES
                     "
     gene            complement(1149..1913)
                     /locus_tag="IW715_000473"
     CDS             complement(1149..1913)
                     /locus_tag="IW715_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1021098.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1941..2639)
                     /locus_tag="IW715_000474"
     CDS             complement(1941..2639)
                     /locus_tag="IW715_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1021099.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2651..3430)
                     /locus_tag="IW715_000475"
     CDS             complement(2651..3430)
                     /locus_tag="IW715_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1021100.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(3446..4390)
                     /locus_tag="IW715_000476"
     CDS             complement(3446..4390)
                     /locus_tag="IW715_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1021101.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4436..5215)
                     /locus_tag="IW715_000477"
     CDS             complement(4436..5215)
                     /locus_tag="IW715_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021102.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(5595..7670)
                     /locus_tag="IW715_000478"
     CDS             complement(5595..7670)
                     /locus_tag="IW715_000478"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1021103.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(7672..8589)
                     /gene="glyQ"
                     /locus_tag="IW715_000479"
     CDS             complement(7672..8589)
                     /gene="glyQ"
                     /locus_tag="IW715_000479"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1021104.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(8975..9793)
                     /gene="recO"
                     /locus_tag="IW715_000480"
     CDS             complement(8975..9793)
                     /gene="recO"
                     /locus_tag="IW715_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1021105.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(9936..10835)
                     /locus_tag="IW715_000481"
     CDS             complement(9936..10835)
                     /locus_tag="IW715_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1021106.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(10850..11251)
                     /locus_tag="IW715_000482"
     CDS             complement(10850..11251)
                     /locus_tag="IW715_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1021107.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(11229..11705)
                     /gene="ybeY"
                     /locus_tag="IW715_000483"
     CDS             complement(11229..11705)
                     /gene="ybeY"
                     /locus_tag="IW715_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1021108.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(11734..13926)
                     /locus_tag="IW715_000484"
     CDS             complement(11734..13926)
                     /locus_tag="IW715_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1021109.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(13950..14921)
                     /locus_tag="IW715_000485"
     CDS             complement(13950..14921)
                     /locus_tag="IW715_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1021110.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            15626..17005
                     /gene="brnQ"
                     /locus_tag="IW715_000486"
     CDS             15626..17005
                     /gene="brnQ"
                     /locus_tag="IW715_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1021111.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(17161..17607)
                     /locus_tag="IW715_000487"
     CDS             complement(17161..17607)
                     /locus_tag="IW715_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1021112.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(17634..17810)
                     /locus_tag="IW715_000488"
     CDS             complement(17634..17810)
                     /locus_tag="IW715_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1021113.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(17972..18400)
                     /locus_tag="IW715_000489"
     CDS             complement(17972..18400)
                     /locus_tag="IW715_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1021114.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            18492..19352
                     /locus_tag="IW715_000490"
     CDS             18492..19352
                     /locus_tag="IW715_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1021115.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(19446..19739)
                     /locus_tag="IW715_000491"
     CDS             complement(19446..19739)
                     /locus_tag="IW715_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1021116.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(19812..21539)
                     /locus_tag="IW715_000492"
     CDS             complement(19812..21539)
                     /locus_tag="IW715_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1021117.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(21752..22678)
                     /gene="trxB"
                     /locus_tag="IW715_000493"
     CDS             complement(21752..22678)
                     /gene="trxB"
                     /locus_tag="IW715_000493"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1021118.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(22823..24256)
                     /gene="ascB"
                     /locus_tag="IW715_000494"
     CDS             complement(22823..24256)
                     /gene="ascB"
                     /locus_tag="IW715_000494"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1021119.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(24256..25659)
                     /locus_tag="IW715_000495"
     CDS             complement(24256..25659)
                     /locus_tag="IW715_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1021120.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            25889..26791
                     /locus_tag="IW715_000496"
     CDS             25889..26791
                     /locus_tag="IW715_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1021121.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(26832..28718)
                     /locus_tag="IW715_000497"
     CDS             complement(26832..28718)
                     /locus_tag="IW715_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1021122.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(28815..29667)
                     /locus_tag="IW715_000498"
                     /pseudo
     CDS             complement(28815..29667)
                     /locus_tag="IW715_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(30002..31786)
                     /locus_tag="IW715_000499"
     CDS             complement(30002..31786)
                     /locus_tag="IW715_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021123.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(31786..33537)
                     /locus_tag="IW715_000500"
     CDS             complement(31786..33537)
                     /locus_tag="IW715_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1021124.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(33679..33903)
                     /locus_tag="IW715_000501"
     CDS             complement(33679..33903)
                     /locus_tag="IW715_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1021125.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34154..35740
                     /locus_tag="IW715_000502"
     CDS             34154..35740
                     /locus_tag="IW715_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1021126.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(35806..37581)
                     /locus_tag="IW715_000503"
     CDS             complement(35806..37581)
                     /locus_tag="IW715_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1021127.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(37792..38694)
                     /locus_tag="IW715_000504"
     CDS             complement(37792..38694)
                     /locus_tag="IW715_000504"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1021128.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            38973..41318
                     /locus_tag="IW715_000505"
     CDS             38973..41318
                     /locus_tag="IW715_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1021129.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        11735 a         8807 c         7422 g        13485 t
ORIGIN      
        1 actctcaaat gcctgttgag ttgtcataac attgatactt gttgtaagta ctgtatattc
       61 tcctatacga ccaatagatt gttttcccaa agcactatct gatacttttg caagattcaa
      121 atgtacagta aggcccattt cagagcatcg ttcaataaat tctttcatac acgaagtact
      181 tttattcaaa ataataaaga cttcatctac ccatccctga cataagtaat ccgctgcatt
      241 tttggaatca gaaacaactg tcactccctc aacatcttca ccaatcatgc ttttatctat
      301 gataatgaca ccatttattt tatacatttt atagttatgc tctttcacat tacgaataac
      361 tacttcagca atactactag atgtaactat aataagtgat ttgttcccct catttttcat
      421 tttatgtttc aagtaatgtt tccataagag gcgagttata tatgtaatta caacgtataa
      481 acctcccatc aagaaaagaa cgactctaga ataatcattt ccaccctgaa cactaatgag
      541 atacaaactt cctaacagca ccaacataat cgcttgcttt acagaagaag caaattctat
      601 atagtaccct ctttttaata cacctctata tgcttcaaac atgaaaataa ctaaaagatc
      661 tgccagttca atgaagactg ccatgttttg atacattgga atcacataag gattccaatc
      721 tacctggcgt aagacatacg atagataaaa agcaagctgg atacacaata aatctaaaat
      781 aataaaatca atatgtttaa cccatcccct tgaacttttc ttgtacattc ctttttactc
      841 ccctcttttt aataaaataa attgtattac ttgttttctc tcctaaatat ctttctgaag
      901 gattattgac tcgacaaatt ggcttgtgga tattcttata tatagaacat ccgtaagcca
      961 acttaaaact ccttaacaaa atttttttac tcatattgta gatcaaaaca agtatccaag
     1021 aaatcatatt ttatttcttt tttctgctta ctcaacaaca gctaaaagaa aaatcatcat
     1081 aaacaaattc aactaagcca acgatcccct tgtgaaatca agttttttct tgatttacga
     1141 ataaagagct aaaataaacc aaacttcttc ttttttacct cttcatagac tggataactg
     1201 atttcatccc cgttgatcag atcctttgcg acttgtttca tctgtactac tttttcctta
     1261 ccaaagtcct ttgcaatctg ctcatagcac tcttttaagt aaaaggtgcg cttagagacc
     1321 ccatgggcat cagaagcagc catctgcacg agattatgtt tgaccatcgt tttggctgtt
     1381 ttctggatgt ctttgccaaa taccccaaca taacttggcg cagtgagttg ggccaaacat
     1441 cccatatcta ggaacggaag caaatgattt gggtcttcgc gaaaatgcac gttgcgttct
     1501 ggatggacga ttactggtgt ctttcctagt tctaacaact caaaaaaaag ccgttctgca
     1561 tataacggca cttccagtgt cggaaattcg atcagaagat atgtatcatc taaatctgta
     1621 aataatattt catctcggtg gatgtcttcg atgagcgttc cggtaatacg tacttcttgt
     1681 ccttccaata acgtcaaagg caactgtctt ttctctaatt cggcttgtaa cgaagcgact
     1741 agagaaatga cttgtgattt ttcattgctg tattttccat tattgtggtg tggtgtgcat
     1801 aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca ttgcaatact tgcttctaag
     1861 ttttctgccc cgtcatcgat tcctggtagg atatggcaat gtagatcgat catttctctt
     1921 gtctcctctc ttcttattcc ttaatttccg tagtaataac cagcatcttt gctatgttct
     1981 gctccgttgt acacaacacc taagactcgt gcttgtacca tctccagcag ttcttttgct
     2041 ttagtcaagg attctttacg tgacatattt tcacgtacga ccaataaagt gccatccgtt
     2101 tttaaagaca tgatctgtgc atctgttacg gctactactg gtggcatatc gaatatcacc
     2161 acgtcaaata actgtcttgc ttcggatagg atctgatcca ttcttggcga gcttaacaat
     2221 tctgatgggt tgggtggctt aggtccacta ggcaaaatcg acaagttctc gactggcgtt
     2281 cgttggatca catctgctac acttcctgat gaactaagtg ctgtacttaa tccactggca
     2341 ttgttcagct ggaatgtctt atagacgacg ggtttacgta aatctgcatc gactagtaga
     2401 actcgttgac ctgatttagc aaaaacgact gcaatgtttg ctgcagtggt ggatttccct
     2461 tctccaggac ctgatgaagt cacgacaatc gttttgattt gctgaccagc tgcggaggca
     2521 aactggatat tcgtccggat cgtacgatat tgttcggcga ttggtgaaga tgggtttgtt
     2581 aatgtgatca aactgactgc atgagtttgt gctgcttttt gtttctgtgt tcgtgccatt
     2641 tttcttcctc ctagactctt gagcggcttc tgcgtgatgg ggctgtttta tcgtttgtag
     2701 cttttggtcg tgcaggagct gttccttttt tacggatacc agcgttcaat tctttcgctg
     2761 acatttctgg aactgttcct aagattggga atcctaatgt atctgtcaca tatttttcat
     2821 ctttcactgt ccgatcgagt aattccaata agaatgccag acctacacct accatcattc
     2881 ctaacattaa gccagtcatt aaattcaatt tattatttgg cgaaacaggt gtcgtattgg
     2941 caactgcacc tgaaatgatc gagattttat caacattcat cacatctttt gcgttctctt
     3001 ggaaaacttg tgaagttgtg ttagcgatct gttgagcagc cactgcattc gtacttattg
     3061 cctgaatcga gaacatttgt gaattttctg cctgattaac tgagatggca tctttgattt
     3121 gttcagcagt cattttcaca ttgtcttctg tttctaaacg atctttgact tcattgagca
     3181 ctaaatcact gacgatcata tctttatacg tattgatcat ttgcaggttc gtattgacat
     3241 cattgacatt tgcagtgtct gattgtggca gtgtcacgat caattgtgct tgtgaactgt
     3301 attttggtgt caagatgaaa aatgttacga ttcctgagat ggccaagcca gcaaacattg
     3361 agatgatgat tattaccaag tgtttgcgta atgtttcaaa aatttctttc aagctgattg
     3421 tctcttccat tttttctcca ctttcctatt tattctctag attcatttga tcttttaatt
     3481 cttttttcac acgatcaagt tcttgatcac tgactttttg ataggagata ttatcttgca
     3541 tgaagccttc tccttgcagc tgatcttgct taatcgtacc aaatgcctcc cgataatcta
     3601 aagcaatttt tttcatttca tcaaagttca tgtctgtttt catattcgac tctagagcat
     3661 tcaaaacgga ttgatagttg ctgacaccgt ttagactcaa tacttttttc gctacgcctt
     3721 ctacgatctt tctttggcgc ccttgtctac cgtaatcacc atttgggtct tcataacgca
     3781 tacgagaata agataatgcc tgatccccgt tcaatgtaat tttaccgatt ggaaagtcat
     3841 agccatcttg tgagaaagtg aggtcattat tgacttcgat accaccaact gcatcaacga
     3901 gttcctttag tcctttcata ttgatagaga catagtgatc gattgggatg tctacatatt
     3961 tttgtacggt atccatcgcc atggcagctc cgccgaatgc gtaagcgtgg ttgatcttgt
     4021 cttttgtgtc acgacctacg atgtcgacat acgtatctcg aggaatgctg acgatcgttg
     4081 tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt atctgatctt ccttgatcga
     4141 cccgacctaa atctccagta tcaatcccca tcaatagaac agagaaagat tcttgtttgt
     4201 ccagatcaac tggtttcgtt cgttctcctt gggttcgttc cactgattcg taagttttct
     4261 caatagaatg tgagagatcc agatataatt ttgctcctac tcctacaatt gctaatacta
     4321 atacgataag aactgctaaa ataatcaaaa atattttttt ccctcttgat tttcttcttc
     4381 gtcttgccat agtttcctcc gtttgctaac cttgcttgaa tcaacgtttt aactgtcaat
     4441 agcttatatt gctatagccc atttgctgag caacagtttg tgcttttgtt gcgtatgcgg
     4501 ctcttatttt ttcattccgg atctgctcta tttgactgag cgccgtcaaa tattgatcgt
     4561 aagtagcggt agctgttact tgaccgtctt tcagcatcgt atcgaatagc tgatctagtt
     4621 gcgtgacttg tgcaatttgg gtatcagcga atcctagata ttgcataacg actgttttcc
     4681 aagcactatc cttaaaaaag ctcatatttt cacgtacatg ggcgacatct gcacttgcca
     4741 acttttcttt gatgatgaca tcatccacag cttgctgcca gtttgatacg tttttagtaa
     4801 ataatttatt gacagccgtc tggatcttca gttttgattc tgcatcctct aactgcgtta
     4861 agactgcttt cttttcttct tggatcgcag cagcttcttt aggtaattct gattccttga
     4921 tgtcataatc atctgccgaa agtttgatag aagatagttt atgtgacaaa tttgaaactg
     4981 tttcttctgt tatttcagga tttaggaaaa ttttttcttt tgtactgtac agcttatcta
     5041 cttcttttga taaagaggtc acggtgtctt tttgattggt gatggcagat tctgcgtaat
     5101 tgatccgttg atgatattgc cgctgattat tgagatggta tccaaacaag caaatcacaa
     5161 tagcgcctgt gcctaccgcc acagtagcca ataacttgac gaacggcttt tccatagatt
     5221 cactcctaaa tattgatgat agataagtaa agatgtgatt cattcacatc agaatgagcg
     5281 ctttttattt taaaagcata attaaactaa tatattcata gaaatattta taaacaggtt
     5341 ttccctatat aatcatacca catacagttt ttttgactag ggaaattcct aatttaaaag
     5401 taaataaaac agtaacaaga ttataattaa tcgttttcat ttttatttcc tcacttttca
     5461 tgagcgtttt gaaaattcga acaaagaata aaataacaaa aattattaaa atagaattaa
     5521 taacagctat ttataacaaa aaaaacagag acacagcacg tttcgtgcat gctctctgtt
     5581 tttcaaacaa aagactattt tgtgatgatt tgatccaagc ttgctaatga caaagccatg
     5641 gcagctattt gacttagttg tgttagtcgg ttcttacgta ctgctggttc atcagccatg
     5701 accatcgttt gatcaaaata attttcgatc agtggacgaa gagcgacaag ctggtcgtag
     5761 ttttcgtgga tcgtttggtc agcaaaactt gctttcattg cttctactgc tttgtgtaat
     5821 tctttctctg cgtcgttttc gaacagctca gggtctactt ctgattgttt ttcaagctgc
     5881 gcttttttcg ctaagttcac gacacgtgtc aaagcttcca ttgaaggttt gaagtcttca
     5941 tctgcaagat gttgtttgaa caattgtgct gctgcaaata cttttgtcag atctttttgt
     6001 tccgcattca aaacagcatc gatgacatca tgacgaatat tcttcgttgt caataattgg
     6061 cgcagacggc ctttgataaa gtcgatcact tgttgttgcc cttctgataa accaatgccg
     6121 aaacgatccg tatctttact gataacatcg ctaatcgttt cttgcagaac agacaaagga
     6181 aaggcccatt ttttcgattc gatgatccga acgacaccgt atgcttggcg acgtaaggca
     6241 taaggatcgt ttgacccgct tggaatcatc ccaactgcaa agaaagaaaa gatgctatct
     6301 aatttatctg caatcgctaa aacagaacct actgtagact gaggtagctc tccttcactg
     6361 gatataggca tgtaatgctc acgaatcgct gtcgctacag caggtttctc gcctttcaga
     6421 agagcatatt tttcgcccat gatcccttgt agttcaggaa attcccctac catgttggtt
     6481 actagatcaa atttatagat ttcagcagct cgttgcaaat ccatcaattc ttcctcagat
     6541 agtcctactt gttcgccaat gatacgggca atgacttgta cccgttgcat tttctcatag
     6601 atactaccga ttttttcatg gaaagtaaca gtttttagtt tttcaacgta ttggtcgatt
     6661 gaagctttct tgtcttcatt ataaaagaat tccgcatctt ctaaacgagc cgtcaatact
     6721 ttttgattgc ctcgaaccac atcttccaat tttacccgat caccattacg agcagaaata
     6781 aaatgaggaa gcagcatacc ttgatcattc cgtacctcga aataacgttg atgttctttc
     6841 atagaagtga ctagtacttc ttcaggaaca gaaagatatt tttcttcgaa gtcgccagaa
     6901 aagacagttg gatattcgac taaattgttg acttcttcta gtaaatcttc gtctagatca
     6961 ataacccagt gatttttttc tgctaattcg tttgcttgtg taacgatcat ttcttgtctt
     7021 ttttgcggag acgcaatgac gaattgttca ttcaattttt cttcatattc ttttgcatgc
     7081 gcaaatgtca catcttcgcc taagaaacga tgacctcgag aggctctgcc tgttttgaca
     7141 tccaagattt cgaatgggat tacttcgtca tctaacaatg ccacgatcca gtgaataggg
     7201 cggatatatt caaaatcgta ttctgcccaa tgcatcgtta cagggaaagt caaacttttg
     7261 atcacccctg ctaagcctgt caatacttct tttgcttcct taccatgagt gtatttcgtt
     7321 acatagacat attccacacc ttttaattct ctaaaagtga tagcgtctgt cgtcagacct
     7381 tgtccgcgga caaatccttc cgctgctttc gtccagtttc catctgcgtc caaagcaatt
     7441 tttttggctg gtcctttgac ttcttcttca ctgtccgctt gtcgttctgg aatatttgtt
     7501 actttgattg ctaaacggcg cggtgtggaa aatgtttcga tcgtatcata agtcaattgg
     7561 ttttcatcta aaaattttgc tgttttttct tctaattgtt tcatgcttgg tgtgacaaca
     7621 tgggcaggca tttcttccag cccgatttca agtaataagt cttttgccat cttattcagc
     7681 ctccttcacg gtttctggat ttgctgtgtt tttattcaac aaagggaatc ctaatttttc
     7741 gcgttctgcc acaaagattt tggctacagc acgagccatg ttgcggatgc gagaaagata
     7801 accggcacgc tctgttacgg atactgcacc gcgggcgtct aataagttga atgtatggct
     7861 acatttcaag atataatcat acgccggatg aacaagattt tcatcgatac agcgtttagc
     7921 ttctttttca aaacgatcaa agttgcttag tagcaagtct tggtcgctta cttcaaaaga
     7981 atatttggaa tgttcatatt ctggttgttt gaagatctcg ccatatttca cgccatcagc
     8041 ccattctaaa tcatagacac tttcaacttc ttggatataa gaagcaagac gttctaatcc
     8101 ataagtgatc tcagaagtta ctggtttaca aggcagtcca ccaacttgct ggaagtacgt
     8161 gaattgtgtg atttccattc cgtcaagcca tacttcccaa cccacaccgg cacagcccat
     8221 ggatgggttc tcccagttgt cttcaacaaa acggatgtcg tgttctaatg gatcgattcc
     8281 taaaaggcgc aagctttcta aataaagctc ttgaatattc tcaggtgaag gtttcatcac
     8341 cacttggaat tgatgatgct gatacaaacg gtttgggttt tcaccataac gtccatcagc
     8401 tggtcggcga gatggctcta cgtaagcagc attccatggt tctggtccga ttgcccgtaa
     8461 aaaggtatat gggctcatcg tacctgctcc tttttctgtg tcataagctt gcatcagcat
     8521 gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc atatcttgga cggtcaattt
     8581 tttactcatc tgttttgccc ctttcaattt taaagcgttg ctgtcaaata agaatacagg
     8641 aaagaaaacg acaaaaaatc cctatgtctg attacagaca tagggacgaa taattcgcgg
     8701 ttccacccta cttccgaata aagattcggc agcttcattt atcgtactcg caagtgcctc
     8761 ttcaaaaatc gtttgggtcc ggctcccact ctcccgaact cgctaaacca cgtgtttttt
     8821 actgatcttg gtcaatgtac gtattcattt tgcagttttc ttgtttctat aatagatcct
     8881 atcactgaac gatctattta atctatggtt aataatagac ggaactccct gtgtttgtca
     8941 aatggttttg attttttgtt gtatgtttta tttactattc ttctttttca ggtgtctctt
     9001 tttctttttc agacatgtct ttctctgacg tctttaatac gtgtgcccaa gacttcattt
     9061 gattgatgaa ctttttgctt ttcaaatgga taccgacata ttcatcatac agctggtcga
     9121 tgagttcacg gattgcttgt ttggtttctt ctttcagatg gattccacta attttatcat
     9181 aagaaatagc tgaaaacatc cgaacgaaat aaacagctcg aggatctgca tgataacggt
     9241 gatcgtccat cgaccaatgt ctttcgcata tcacaccacc atatttggaa gaatagtcaa
     9301 attttccttt tgtttccccg catacgacgc agtgtgtcca gattggtgca attccgaagc
     9361 gttgcagtat ttggacttca aaaatattcg tgatgatttc tgcatcgtct ccttggtcca
     9421 tccgttgcag tgcttgttgc gtaaactgga acaaatgcgg gtcgtactga tgatcttcga
     9481 ttgccgcatc tactagattc aataaatatg tgccatagcc gctcaaaaag atgtcttctt
     9541 ggatgtgtcg aaacgcatgg acatctttga cactgcttaa aaatgacaaa ccttcacttc
     9601 gaaaattccc gatataaact gcttcagtaa aagggagcaa agccggagct aagggctgat
     9661 tttgccgatg gatctctttg atataaaaca tcattttgcc agaagattct gtaaagattt
     9721 tgactaattt atctttttct ttgtaattac ggctagataa aatcaagcct tttgattctg
     9781 caagctggct catattctcc ctcctaaaaa ataaatctgg aaaagcagat tctgccgatc
     9841 cagatcattt atgttgcgat cttccgcagc ttttctcaac acgcggattt ttattcagtt
     9901 tatttctata cctaatttac aagaagctca gaatatcagt attcgtcttt tcggtagccg
     9961 aagtcttgca gatagacttt tttgtctcgc cagtcttttt gaactttgac ccaaagttct
    10021 agaaagactt tgtctcctaa aagattttcg atatcttttc ttgctttcgt tcctacttgc
    10081 ttcagcattt ttccgccttt gccgataatg atcccttttt ggctatctct ttcgacaata
    10141 atcgtagcct ggatatggat tttatcattc tcgtttcgct tcatcgaatc aacaacgact
    10201 gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga ctttctcacg gatcaattca
    10261 gaaacgatga agtattcagg atgatcagtc acttgatcat ctggaaaata ttgtgggccc
    10321 tctggcattt cactgactaa tacatccatc aaacgttcga cgttgtttcc ttctgtagca
    10381 gagattggaa tcacttgtgc aaaaggcatc tgcgttgaat aatcttcgat gattcccaac
    10441 aactcgtctg gatggatttt gtcgatttta ttgataatca gataaacagg agaatgcacg
    10501 ttttttagac gttcaataat aaaatcgtct ccccgaccgc gtttttgatc agcactaatc
    10561 ataaataaga ccgcgtccac ttctttcaac gcactgtaag ctgcttctac cataaaatct
    10621 cctaaacgat gctttggttt atggattcct ggtgtatcaa taaaaatcaa ctgagcttct
    10681 ggtgtagtgt atactccttg gattttattt ctagttgtct gtgccttgtc actcatgatg
    10741 gcaatttttt gacccacgat tcgattcaat aatgtggatt tccctacatt tgggcggccg
    10801 acgattgcga caaaccctga tttatgttct ggcatatatg ttctcctttt taattaaaca
    10861 acaaatgata tattttaggg acaaataaaa tcaacccaac gatgacggta aaacaagcgg
    10921 tcactaaaac tgctccagct gccatatctt tgattttttt cccaatcgga tgaaaatgaa
    10981 aatccgtaaa catgtccacg acattttcaa aaacagtatt gatgatctct actatccata
    11041 cgagaaaaac agctaaaagc agccataacc attctatccg atcaagttgg aaaatcacgc
    11101 ccataaacaa agcgaggatt ccgaacgcaa catgtttcct catgttgcgt tcttcctcaa
    11161 aaacagtttt gactccttgg acggcaaatt ccacggacat caggaaatgt ttgtttttct
    11221 ctacgttttt atcttttaag tccataggca tctaagatct ctttctgtaa accaaacatt
    11281 tctttctcat cctctggtgt catatggtca tacccgttga tatgcaagaa tccatgaacc
    11341 gctaaaaagc caagttcccg atcatacgaa tggccgtaag cttctgcttg ctctgcagca
    11401 cgttcagtag agatcatgat gtctcccagt acacgaggca atggattctc tccatcttca
    11461 aaaatcactg ggatttcgtc ttcgccctct tcttccaagg caaaactgat gacgtctgtc
    11521 ggtgcatctt ttccacggta atcacgattg attacttgga ttgctgcatt gtccatgaaa
    11581 gtaacagaca tttctgtttc ttctggcaac tctaaatatg atgctgcaaa ttgcagcaag
    11641 tcgtcgattt ctttcatttt ctcttctgag acttggtttg tttcatccat gaaggtaata
    11701 tccattatac atcgcttctt tctattcttc ttgctattct tcttgttctt cttttatctt
    11761 ctcaacttcc gatttcattt taggatattt gatccgagaa tggaaggtac tgcttaaacc
    11821 gctgatcaat gatttttcta ctttacggat ttcttttaac gtcaatccac tgtcgtccaa
    11881 ttgtccatcg gaaatacgct cttcaataag attatggaca aaactttcga ttttttcgct
    11941 cgttggatgg tccattgccc gtactgccgc ttcacaactg tcagctatat tgacgattcc
    12001 tgcttcacgg ctttgaggtt ttggtccagg ataacggaat tgctcttccg taacttccgg
    12061 gttgcgttcc tttgccttaa cgtagaaata acgcatcagc gtggttccat gatgctgtct
    12121 acaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg caccatctgt
    12181 cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt gcggattttc
    12241 cgctcctgaa ggcaagttct caacgaaaaa gctggcgtgt ttgatcttgc cgatatcatg
    12301 ataatagcaa gctactcgag ttagtaaaga acgtccaccg atttctgcta cagcattagc
    12361 acttagattg gctaccatca tgctatggtg atacgtacct ggtgcttctt ccagcaactg
    12421 tttcaataaa ggatgattgg gattgctgag ttcatttaat acgatcacac tatcatcatt
    12481 gaccatcaat tcaatatagg ggtgcagacc tatcgtcagc aagaatgaaa acagtgcacc
    12541 tgtgaatccg cagactagcg ttaaccaagt cgttgcatcc ccaaagctca ttccttggta
    12601 gataatcaaa atgacatcca tcatcacagg aaaaataatg acccacatag cagcggataa
    12661 cccttgttct gagatccgtt tgcgtctgac gatcgttgcc atgagaccag aaaatacata
    12721 ggacatcaaa ataactgtca gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc
    12781 ggatactgct tgaaaaatac cagccatgat ccctgctctt cgattcaaga aaaagcttaa
    12841 aacaagtgga gcaaaagccg cggggtaaaa cagcggaata taagcagctt gttctgtttg
    12901 gaagagttgg aaaaatttca ttagtacgac gcttactgac attgccgtca cgtagaaaag
    12961 tacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa tcagtacacc
    13021 gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatagaag tcgtttgatt
    13081 cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat ttccttcgcg
    13141 aacaataatt tctccttgga agatcatcac tggttgtaca gcttctcgtg cagattgttt
    13201 taattcttct gtctttttct cattcaatac atcattaact acgatgcctt gatccactaa
    13261 gtagcgaatc atttgttgct gctctgttgt gacattcaaa tattgaatct gatcttctgc
    13321 ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct gttcgtcaat
    13381 gagcttcaaa ctttcatccc taactgtgtg aatttccgtt tcagtcattt caaatatcgt
    13441 cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat tcacgttttc
    13501 aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga tattttcatc
    13561 ttccttcgcc ttatctacct tgccattata agatttgttg atggtatcat tcgtcttatc
    13621 gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat ctttttgata
    13681 agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg tttcttccgt
    13741 gttctcaatc gttttattcg cacggatact ctcttctgct acttggcctt ctctgaaatc
    13801 aatggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa aaaacaagaa
    13861 cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca gtgttttatt
    13921 caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc cttttcataa
    13981 gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc aaaattaaca
    14041 aaatcgattt ttttgatttg ctttaatgtt cgttctgcat gaacaagacc acttgtcgtc
    14101 ccacgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga ttgataaccg
    14161 agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc gtccaagata
    14221 acgaatgcat catccaatgt ccgcccccgc atgtaagcta aaggtgcgat ttcgatcacg
    14281 cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa agcatcataa
    14341 acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa gcctaggttt
    14401 tcccctgcct ccacagcggg ccgcgtcaaa atgatttttt gcacttcgcc tttttttagg
    14461 gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg ccctacacca
    14521 aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct gttttttacc
    14581 cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc aacaaaataa
    14641 tctaatgtac ctttttgccc cattttcaat gccgtcacaa catctggcgt acttacgtgg
    14701 attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa ggaaatctgg
    14761 gattgctcac caatgatctg aatgatttct ccacgtgtat ggatggttgt ttccgttgtt
    14821 tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca
    14881 gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca tccccttttt
    14941 ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca aataagaaaa
    15001 ggttgtaaaa gagtcgctta accatacgta ccaagaaacg ttaaaaaata gccgtttcca
    15061 ttcgctaaag gtctcgtttt gataaaaaga agctgagaat gtaagttata tgaaagtgga
    15121 gaacaaaata tttcgtttca ttcaacaagt attctctagg tatttttcct gtttcaaaaa
    15181 aagaaaacaa aatagttaaa atattcttgc atttcaaaaa aaacaggtgt attattacgt
    15241 caacaaataa acatacagca aagcgtcgag gaaaacagta ggctattcaa cgatttattc
    15301 ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat agacgaacat catttccgag
    15361 tcaatgtcga aaacgtagat cattgtggct gcctccatta tcaggctagc ggataacttt
    15421 atcaaaaagc gtccgtgaat gaagaagctt ttttaagcga aaaaaggtgg taccgttcta
    15481 gaatctagag cccttttccc aagtggaaaa gggctttttt ttattttaag gtggtggttt
    15541 tcatggaatc tgatattagt ggcattacta attgctgcaa cgtttatatc gtcaatcaaa
    15601 atgataatta ttggaggaaa aatttatgga agaaacaaaa acttattcat ggaaacaact
    15661 attgatggtc agttcactta tcttcgggat gttcttcggg tcaggtaatc taatctttcc
    15721 tgtccactta ggtcaaatgg ctggcgcaaa ctgggtcagt gctggcgtag gatttgctat
    15781 ctcaggtgct cttttcccat tactagctat cttagctgta gtagtgacaa aaagtgatgg
    15841 tctgtatgac ttagctaaac ctgtcggaaa atggtatgct gccttgttct taatccttgt
    15901 tcatttgacg atcggaccat ttttcggtac accacggaca gctgctactg cttttgaaat
    15961 ggctgctaaa ccatttttgc ctgaaaaata taattcatta ggtatgtttt tattctctgc
    16021 tcttttcttt ttacttgctt atttccttac tgtccatcca acacggttgg tcaaatacgt
    16081 cggaaagtat ctaaactcct tgtttcttgc attattagca atcatttttg tcgtttcttt
    16141 attccatcca atgggaaatc taaatcaatc tgctgcagat acttatcaaa caaacgcttt
    16201 aacaaatggt gtcctagaag gttataacac ggttgatgca gtagctttgc ttgccttaag
    16261 tgtcactttt gtccatgcag tccgtgacct tggtttcaaa gataaaaaag tcacagaatt
    16321 aacggcaaaa gctggtagcc tgagcgttgc gctagaagtc atgatctatt ttggacttgt
    16381 tcttctcggt gcgatgagtt tgaataagat gtctttatca gaaaatggcg gaactgcact
    16441 ttctcagatc actaattttt atttaggtaa cttcggcgct agcttcttag gagtgatggt
    16501 cacattaggt gtcttcacta ccgctatggg actcgttgtt tcttttgcgc aagatttcca
    16561 taaactgttc cctaaagtca gttatatgac atggctgcgg ttaacgactt tcgtctcttt
    16621 tgtcgttgca aatgctggtt tagacaatat catccaatgg tctttaccag tattgatgct
    16681 tttgtatcca ttgtcgcttg cgttgatctt gctttcctta acagcaaaat ttttccaaaa
    16741 gacaccattt gtctatcaag taacgatgtt gtttgctgct gttcctgccg tattggatat
    16801 gctggcaaat tcgcctgctt tagtgagtca acaaagagtt gtagcaagca tgctcgaatt
    16861 ttatcatcat cacgttcctt ttgctgcatt aggacttggc tggatggtcc caacgttact
    16921 tggttacgct ggcagcctac tgttttatta cgcctatcgc ctatcagggt ataaacaaga
    16981 agcaaatgaa ctaccagaag aataaccaaa tcaaacacaa tttaacataa gaaatattaa
    17041 acaggaaaaa accagctaga tgaaagaatc ctattctttc gtctagctat tttttagttc
    17101 tcttttttac gcgcacaaaa aaactgcgga caatgattgt ccgcagttta aatgataaaa
    17161 ttaagattgt aatagttctt tcacaatggc atttacttgg tttccatctg cttttccttt
    17221 gactttaggc atgatcgcac ccatgacttt gccaaattct ttcattgatg aagcaccagt
    17281 ttgtgtgatt gcttcttgaa caagttgtcg aatttcttcg tcggaaagtt gttccggcat
    17341 gtatttttcg actatcacaa tttcactttt tactttttca gctaaatcgt cacgtcctgc
    17401 ttcttcaaac tcatgcaaag aatcacgtcg ctgtttcatt tcgcgagaca acaccgttaa
    17461 ttcttcttct tctgtcaagt cgtgaccttc tttaatctgc tcgttttgta tagatgactt
    17521 gatcattcgg atgacttgta aagattcctt atctttcgct ttcatcgctg ttttcatgtc
    17581 atcgtttaat ctacttagta gtgacacact accaactcca attcatcaca gaactagaat
    17641 tttttgcgtt ttctagctgc ttcagatttt ttcttgcgtc ttacacttgg tttttcgtag
    17701 aattcacgtt tacgagattc ttgtaaagta cccgcttttg acacggaacg tttgaagcga
    17761 cgaagagcat cgtcaagtga ttcgtttttg cgaacgactg tttttgacat gttaattccc
    17821 tccctccgag cttaaaaagt aaccgtaatt gttattgaaa tatatctcaa aaaacaagac
    17881 tgtcctgtaa atattatatc taatgctgtt ttgaccgtca aggaaatctt gctttttttt
    17941 aaaattatca aatgttattt tatttaaatc cttattgaca attttcacat cttccgaaaa
    18001 tctcgaatct atgcccttcg atcgtacatc caccgagctg ttcttcgaaa taattcattg
    18061 gacacatatg gatttctttc gttgttccgc acaccgtaca gataaagtga tggtgatggc
    18121 cgatttcccc ttgacagcaa tgaaaacgaa acttcatctc tccatttaaa tcggtttctt
    18181 ccagtaaatc gatttctgca aaatcatgga gatttcgata aatcgtatcg taacttactc
    18241 ctttgaattc ctgattcata tagtcaaaaa cttctttagc ggatacgtag cgattgcgct
    18301 tcactaaata agttaacaat gcttctcttt ttttagtata tttaaaaccg ttatttttca
    18361 atgtttctag tgattgttca atcaatgtct gttttttcat gacttattcc tccaaatcaa
    18421 aatgattacg aagtacacat tctagagtat ggtataataa acataaaaaa aatgcaagtg
    18481 aggttttatc catgtcttcc gattctacag aaatcattac tttttttgtc atctctgact
    18541 ctgctggaga aactgcgaca aaattagcgc aagccactat ggcccaatat cctaccgttg
    18601 agttcaattt attccgcaga acatttgtga cagatgagaa aacattgatg caggcactgc
    18661 aagatgcact aaaagaaaaa gcccttgtcc tacacacatt gatcaatcaa gaattgatcg
    18721 agatgacgcg ttctttttgt gaagaacatc aattatttag ttttgatgtg atgactccgc
    18781 cgatatcaga aatcgagcgc ttaactggta tcaagcccat tcggcaacct ggcgcccttc
    18841 atttattgaa tgagaactat ttcaaacgga tcaaagcaat ggaatttgct gtaaaatacg
    18901 atgacgggaa agatcctcgt gggtttctag aagcagatgt cgttttgcta ggcgtatcca
    18961 gaacttccaa aacgcctttg agcttgtttt tagctaacaa aaatttaaag gttgccaatc
    19021 tacctttgat cccacaagca catattccca aacagctttg ggagatcgat cctaaaaaaa
    19081 tcgtgggact gacgaataac ccagatattt taaacaatat ccgtaaagaa cggatgcgct
    19141 cttacggatt aaatcctgat actgcttatt ccgatatcga aaaaatacga gctgaactcg
    19201 attttgcgaa tgatctttat gaaaaattgg gctgcgtagt aatcaatgtc gcttctttat
    19261 ctatcgaaga aacagcttca cttattttga atgctttaga cctagaagat catagttatt
    19321 atggaactga aaccaccgaa gaaacagact aacagttttt cttgtctttt tcttaaaaaa
    19381 actggactgt gtcagcgaag aagattcgtt gatacagtcc agtttgtctc tatttgcacc
    19441 tatcgttatt gatgttccac atgtttgagc gtctggttca aaatatccag tacgtgagaa
    19501 tcatcaagac tatacaaaat cgcttttccc tcacgatggg acttgacgac atggttttct
    19561 cttaataaac gcagttgatg cgatacagca gattgttcca tttctactgc ttgggtgatt
    19621 gctgtcacat tttgttcccc gtctttcaga tacagcagaa ttttgagacg agtggggtca
    19681 ctcaacattt tgaatagttg gctgactttc tctacttttg ttttttgcgt gtctggcaat
    19741 ccattcacct ccaacttgaa aaaagactga cttctaaagt ttaccacttt ttccatcagt
    19801 ctcaagtttt tttattcttc tgtcattttt ttgataatcg cttcataagc ggctactttt
    19861 tcagctgcgt cttctgaaga gtttcctttt acaccgatat aaaatttgat tttcggctca
    19921 gttcctgaag gacggatagc gatccagcca ttttcttcca gcgtatactt caatacgtca
    19981 gatggcggtg tcgttagttt ttctacgttt ccttcacggt ctgttctcgt caactctttg
    20041 aagtcttctg tttggatgac ttttgctccg gcaaattctg tcggtgcttc ttcacggaat
    20101 ttttccatca aagcagagat ttttgcagag ccttcttgtc cgctcattgt cacagaaatc
    20161 gttttttctg cgtagtagcc atatgtttca aaaatcgttt gcaagccgtc atacaatgtt
    20221 ttgccttgtt ttttatagta agctgctact tctgctaata agagcaatgc ttgaatcgcg
    20281 tctttgtcgc gaacgaaagg tttaactaaa taaccgtaac tttcttcgaa tccgaacata
    20341 aaagtatggg aatgatcttc ttcaaattgt tgaatttttt cagcaatgaa tttgaatcct
    20401 gtcaatacat tgaacatggt agccccatag tgttccgcaa ttgctgtagg caattcgcta
    20461 gaaacgattg atttcaatac cgctgcattt tcaggtaaag tgcctcgcga ttggtgtgct
    20521 tccaaaatat attgagcaaa cagggctccg atttggtttc ctgtcaatac ttcataggag
    20581 ccatttggca aacgaacggc tgcgcctaaa cggtctgcat caggatccgt tgcaatcagt
    20641 aagtcagcgc cttctttctc gccaagtcgc atcgcatact cgaaagcaga gtgttcttct
    20701 ggattcggtg atttgactgt agtgaaatcc gggtctgcga cagcttgttc tggaactagt
    20761 acgaattgtt cgaatccagc ttgtttcaac gcacgttcgc ccaacatttt tcctgttccg
    20821 tgaagtggtg tgtacacaag cttcaaatct ttacccattg tttccaccaa ttcatgatca
    20881 atcgttactg ctttgatttc ttctagataa gcggcatcca cttcttctcc gatgatcgtg
    20941 atcaatccgc tatgttcagc ttcgtcgtca gataatactt caacttttaa cggattgctg
    21001 acttcacgga catatttcgt caatgcatct gcgtctgcag gaggcatttg tcccccatct
    21061 tctccgtaga ctttatatcc gttgtaagct gctggattgt gggatgcagt taccatgatt
    21121 cccgcaaacg tcttcaaatg acggactgca aaagaaagct ctggtgttgg acgtaaactt
    21181 tcgaatacat aagaagggat attgtgatgt gctaatgttt tggctgcttc catggcaaat
    21241 tctggtgatt gatggcgtga gtcataagcg atagcgacac ctcggcgttt tgtttcttca
    21301 ccttgtgtat ccatgaaacg tgcaagtcct tctgttgctt gtcttaccgt atagatgttc
    21361 attcgattga tcccaggtcc taagattccc cgcatgcctg cagtaccgaa ttccaacggt
    21421 gcataaaaag catcttcaag tttttcagga tcttcaccca gatcatttaa ttgttttttt
    21481 aagttttctt ctaaattttc ttcatgtacc cattgttcat acacttgttc ccaagacatg
    21541 aaaataacac ctctttctga aatatggttc ttaataagta tagcattcac ttattagata
    21601 tgaaagtatg aggaccaaaa aacgcttaaa tcaagaaaag attcttttat caagcacggt
    21661 tacaagtatt cgcataaatc ggattcagta gatttcacgc aaaaaaaaga gtaaaagaac
    21721 aattgaaacc gttctttcac tctttttaaa cttatgcagt tgtttcttct aaagattcca
    21781 agtatttata cacttgttgt ccagcaatcc caccatcacc aacagcagta gtgatttgtc
    21841 gcaaatcttt ttcacgaaca tctccgatgg caaaaacgcc tggtatattc gtcttcattt
    21901 cgttgtctgt tggaatccag ccggcttcgt tcgtcaaccc agcttctcta aatggtgcag
    21961 ttaatggatc caatcctaca tagatgaata ctccgtctgc tgccaaatca aactcattgc
    22021 cagttttgac attacgtgct ttcacacctg tcactaccat ttcatttcca acgatttcgt
    22081 caacgactgt atcccacata aaggaaattt tttcattcgc aaatgcacgg tcttggatga
    22141 tcttttgtgc acgtaattca tctctacggt ggacgatgac tacttcagaa gcaaattgtg
    22201 tcaaatagat ggcttcttct actgctgagt ctcctcctcc gatcactact agacgtttgt
    22261 tacggaaaaa tgcaccatca caaactgcac agtaagaaac gccacgtcca gcaaattctt
    22321 cctcgccagg tacgcctaat ttacggtgga tacagcctgt tgcgataatg actgtttttc
    22381 cttcgtaagt tttatcttca caaataactt ctttgtggct gccgtgatcc ttgatatctt
    22441 gcacgattcc ataagcattt tctgtcccaa atcttgaaac accttcatac atcttataag
    22501 ctagatcagg acccatgatt gaatcgaaac caggatagtt ttcgatttct gccgtattgt
    22561 tcatttggcc gccaggagcg ccttgttcga tcatcaatac ggataagttt gaacgtgaag
    22621 catataaagc agcagtcatc ccagcaggcc ctgcgccaat aacgattaca tcatacatat
    22681 gtagattctc ctttacttaa tcaagtaagc atactttaca gccaatgcga aaaaaagtct
    22741 attgttctgc ttgcgtttct cgaatagctt tattatactc gaaaaatccg ctgaaaaaca
    22801 atagactttt ataaatcatt gatcaatcgt tcgaaagatt ttctccattt gaatcaatta
    22861 cttttttata ccagttgaat gatttcttct tcgaacgggc taatgtgcca tttccttcgt
    22921 tgtcacgatc gacataaatg aatccgtacc ttttcttcat ctcacctgta ccagctgata
    22981 ctaaatcaat acatccccaa gtcgtatagc ctaatagatc gactccatct aattctactg
    23041 catctttcat tgcctggata tgtgctgcca gatagtcgat tcgataatca tcttccacat
    23101 aaccattctc atcgggcgta tcgactgcac ctaagccgtt ttctacaata aatagtggtt
    23161 tttgataacg atcataaata tcattcatcg ttattctcaa gcctaatgga tcaatttgcc
    23221 aaccccattc acttgcttcc agatatggat ttttgacaga agcaaagata tttcctgcag
    23281 tctgttccaa gagttcgttg tcagtagtcg aaactcgaga agaataataa gaaaaggaga
    23341 taaaatcaac agtatgttct ttcagcaatg cggcatcccc aggttccatt ttgatctgaa
    23401 tgccatttcg ttccatttct ttcaatgcat aagcgggata ttctcctcgt gactggacat
    23461 caatgaagaa ataattttct cgatctgact ttctagaagc ccaaacatct tcaggattgc
    23521 aagtgtatgg ataatatgct cctgctgcca gcatacagcc tactttgttc tccggatcta
    23581 cttcatgagc gatcttcgtt gcgatggcac ttgcaaccaa ctcatgatgt gccgcttgat
    23641 atttgatttg ttcttcattt tctccttctt caaaatacag ccctgcgccc atgaatggtg
    23701 catgaaggat catattgatc tcattgaatg tcaaccaata tttgacaagt cctttatagc
    23761 gattgaagat gactcggcac agattttcat aaaaaccaac gagttctctg gaacgccacg
    23821 caccatattc tttgatcaaa tgcatcggac aatcaaaatg tgtaatcgtc acaagcggct
    23881 cgatcccgta cttttgacat tctttaaaga catcctcata aaatttcaac ccagattcat
    23941 ttggctcttt ttcatcgccc attggaaaaa tacgactcca agcaatggac atccgataag
    24001 tcttgaaccc catttctgca aataaagcaa tatcttcctt ataatgatga tacatatcga
    24061 tcgcaatttt tgctggataa aaatgatttt catcaaattc aaacattttc ttttttccag
    24121 taatgacagg aaaacggtct tctccgattg gcacaacatc tacgttcgct aacccacgtc
    24181 cgccttcatc ataaccgccc tcgcattgat tggctgctgt agctccgccc cataaaaaat
    24241 cttttctgaa tcccatcata cttcctccat tacatgtgta ttgcttgata attctcttgc
    24301 cagttcatct tggacttcga ttccttctgc ttttaaccgg ctgattgttt ccttgaattg
    24361 cggtaaatac tgctgatgtg caataaacaa ctcatccatg atccgttttg ctgtctttcc
    24421 tgatcgtata agcggattga tcgtaaacgc ttgcaaagct gttccataat caccagtcac
    24481 ggctgcttcg atcgtcagca attccatatt cttcattact tgcaaccagc ctctttcagc
    24541 agcaggaagg gaaccaaacg ctacatttcg tgcgccttgt gcgccaacat aggcagtgac
    24601 ttctactaca caatctgccg gcagatcagg aacagctcca tcattctttg tagataccac
    24661 gatctgtgtt tctttatttc catagatcga agcaatcgtt tcacaggcag catctgaata
    24721 ataagcacca cctcgttttt gcaattgttc aggtttatga tccaattctg gatctttgta
    24781 taattcaaac agctctgctt ctgtttcttt cacttgctgc gctcttgtac caatcgtttg
    24841 atattcttct aaagcatgtt ccagcatttc ttcttcacga tagtaatagc ggtggtagcc
    24901 acaagggatc atgttcattt gttttaattg ttctttgaaa aacgggatgt caaagatatt
    24961 tttcggcaat ccattgtctt catcgtataa acgatcaatc aattctgccg tgcggtcatt
    25021 tcctttatta tctgctactt tgtgccaatg gaagtgattt agaccggcaa acttgtagat
    25081 cagctcttct tttttcttgc caagcattgt tggttctgtc atcatcgcca ttactggcac
    25141 attgcataat ccgatcactt tatcccattt gccatatctg ataactgctt ctgtcaccat
    25201 cccgcttggg ttcgcgaaat taatcagcca agcatttgga cataaacgct tcatgtcttc
    25261 aacgattgcc aaaaccactg gaacggtacg aaaagctttg aatatcccgc cagcaccatt
    25321 tgtttcttgc ccaatcatcc cataataact agggatccgc tcgtctttga ttcgtgcatc
    25381 taattgaccg acacgaaact gagtcgtaac aaaatccgca tctttcaatg cttcttcccg
    25441 atccagagtt gcatagattt tgacttcata cggagaagca tcccacatac gctgagacat
    25501 ctgactgacg atctcaagct tttcttttcc ttcttctaca tctacgagcc agatttctcg
    25561 tatcggtaat tcctcatatc ttttgataaa tccttccatc aactcggggg tgtaactgct
    25621 gcctccacca atcgtagcga ttttgattcc ttttgccatg ttctcactcc tttacttttt
    25681 acacttttat tatctttttt gtaaacaaaa caaacaatag aaattcgttt ctttccagta
    25741 acatttacaa aagttagtgt tttcttctgg atttttgtaa atatcttgtc aaaaaatgtc
    25801 tttattaagc tatataacat aaatccgttt acaaatcatt ttcaaaaaag ttataattta
    25861 cgtaaaaaaa caagaagaag gtatctacat gttattacgt gaaaaaatca aagaaatctc
    25921 cttttctcct tcagaaaatg aagtgattca gtttctctta agcgattctc cagcacttgg
    25981 cgaaatgacc atccaagaga ttgctgagcg atgttatgtc cacccgtcca cactcatccg
    26041 tgtagcaaaa aaaatgggtt ttgatggctg ggtcagtttt cgtaatgctt ttttagctga
    26101 aacagcctat cttgaaggga attttcaaga cgtagatgcg aatcttcctt tttccaccac
    26161 agacggtatt atgacgatcg caaataaaat ggcttcttta gaacagatga cgattcaaga
    26221 tacactttct ctcattcatc atgaccaact gcaaaaagcc aaacaactaa tgcttcaagc
    26281 aaaaaaaatc gtgattttcg ccagtaactc gaatactttg atttctcaag atttcatgct
    26341 gaaaatgaag cgcatcaaaa tggatgtcac gattgtcacc acttttggtg aatcgttcta
    26401 tgaagcgtac aattgcacaa gtgatatgtg tgggattctg atttcttata cgggtgaaaa
    26461 caatgtgatc cttcgtaccg cagaaatatt gaaacaaaat catgtgccaa ttttagcttt
    26521 gaccagtatc ggtgattcaa cgctatcttc aatgagtaca gccgctttgc gacttacgac
    26581 acgcgaacga ctgtattcga aaattggtaa ttttacgatc aatacatcga tttgttatct
    26641 tctagacgtg ttatatagtt gtgtttttgc agaaaattat caacagaatt tagtccatct
    26701 aatttctgta ggacaaaaat ttgataaacg ccctatcagc tcagccatca tgaaagagcc
    26761 tttcaatttt gaaaataaag aagtggacta aaaagaatag ttaagcctag tccattcttt
    26821 cacctaaccg ttcaggtcag tgcggtgata agtgcatcgt ctggcccgac cgtttcccct
    26881 tgattttcca aaatatccaa ataatccatg taattgatga caatcacagg tgtttctaaa
    26941 ctgtatcctt cctcttcgat tttctttttg tcgaagcgaa gcaacagatc gccttgtttc
    27001 actctttctc cttgctgcac caagctttca aaatatttcc cttccaactg aacagtatcc
    27061 aatccgacat gaatcagcaa ttccagacct gcttcagaaa ccatcccgat tgcatgtttc
    27121 gtcggaaaca gggacatgac tgttccgtca aaaggggcac gtacttctcc tttttcaggg
    27181 tagatcaaga ttcctcgacc tagtgttcct tgtgcaaaag ctgcatcctt tgctgcattc
    27241 agtggagcga tttgtccttg tatcggtgtt tttacaattt ctttacctga tcttttctgt
    27301 ctatcatctg tttcatctgc ttcctccttc caaaagaaaa aagtcagcgc aaaggatacc
    27361 accatcgcaa tggcgatccc aatcaataca ttaatcacat ctgttaaatc accatctggt
    27421 ctaatatagt tgggaattgt aaagatgcct agtccacctt gattccaaga agttaaattc
    27481 attaacccac tatacgcgcc ccctaacgca ccaccgacac aggtaaaaat aaatattttt
    27541 ttacgtggca atgtaaaccc ataaatagct ggctctgtaa tccccacaat accagaaaca
    27601 atggctggta ttgcaattgc tttgtctttt ggatttctaa gcttaaagta gagtgcggta
    27661 agtgctgcag tttgtccaaa agacactact gcaacaggtg tcataatatt ttgccaacca
    27721 ttcacagaaa attgcatcaa tcccattggt acgattgccc aatggagacc aaacatcacc
    27781 agaatttgcc agaagaagcc tacgattgct ccataaataa ctggcgaaag tgctaaaagt
    27841 gtttcaaatc cagccattaa aatatcagtt gcgaatgtga atactggtcc aacgagtaaa
    27901 aaacctaaag gcattgtaat aagaacggtg aagaatggaa cgaagaaatt cgcgatcatc
    27961 tcaggtacta actttttaat tggtttctga atttggctag ctaacaacac aataaagata
    28021 atgggaatga cagaagaagc gtagttacgc gcaatccagg gaatgccgaa tactgtttga
    28081 taaataggtg cttcaaaaat agtgccagaa aatatggtcg ttagtggtgc ttctgctgca
    28141 ccactcaaag cttccagcga caagctagga ttcattaatg ctgcgccaag caacatccct
    28201 acgaattcat ttactttaaa tttacgggca gctgttaaac cgataataac aggcataaat
    28261 tggaatacag aatcaccgat attatcaaac ataacaaatg tgccagaacc gcggtcaaac
    28321 gcgcctaaga agacaaggaa tgctgtcagt ccacgaatca tcccgtcagc agctaggata
    28381 gcaaggaatg gttggaaaca gcccgatatt ccatcaataa gcatatcaaa cggtctcatt
    28441 ttttctgttg aaacagcctc atcaaaagta aggtttcctg cttttataac ctcttcaaac
    28501 acgactggca catgattccc gatgacaact tgatactgcc ctcccgctcg cattaccgtt
    28561 acaataccgt ccatattttt caagatctca tcatttgctt tttcttcgtt ttttaatttg
    28621 aaacgaagcc ttgtgataca atgtgtcaaa ccattgatat tttccttccc gccgacattt
    28681 tctacgattt ttctcgctaa ttcttggtat tttcccattt ctattccctc caatttatta
    28741 gtggaaggtt tagccaactg aatgtcacta cccttatttc atacgttctt gttaatttat
    28801 cctccttttt ttatttattt acaacgattc tcatgcacta aacgcgcagt atggatcgtt
    28861 aaatacattt tttcgtccgt ggacatctgg tagttgaacg tctcggctaa aaagtctgct
    28921 attttctcaa cgcaaccaaa cgtatcctta tatttttttt gaataacctg aaacaattcc
    28981 tcagaaactt ctcccacttc tgatttttct ccatctagga cgcgtgtcag aaaaaaacgt
    29041 aagtgtgtga cgaatcgaaa aaaatggact gaatcttcat caaatgtaat cttatagtaa
    29101 tagcggacga tatttagaat atcctgtatt gttatagtga acaggtaggt attgtcttgt
    29161 tggctttgaa tgccttgtgc attgactaaa tgcaatgcga taaatccggc ttcatcttct
    29221 gataacttga tgtggtattt atccgagatt ttcttcactg ttttttttcc cgatggcata
    29281 ctctttagga aatagttgtt tgatatcata cagcaataca tttggtacaa atattcccat
    29341 tttgtgacgt tcgatagccg tatacaaatg atccgtcaat gcaatataca cagaatcatt
    29401 caactggact tgtagagcct cttttgtata ttcaatgatt tcacaagaaa tttctaagta
    29461 ctcaagagga atcgcttgtg ccaattcttg gaactgttgt gaaagcccgt catttgtcaa
    29521 ctgaaatttt ttgtcgatgc atgagtcaaa aatcgggtca cccactttct ttttgaatgc
    29581 caaacctttt cccatcacga tcatttcctg atttctttca tccctggtta ttacgacgtt
    29641 gttattcagt actttctcaa tcagcatgtt ttgctccttt ccatgatata tagggctgta
    29701 tccaaaacaa aaagaaaaac ccaaaaagcc gtccatggaa ccatgggaga ctcatcaggt
    29761 ttagcttgca tcaggcaatc actatccaag ttgtatcgtt ttctttatat tatcgaaagc
    29821 aaacgctttt gtcaacgctt tcttagtggt tattagccgc ttttgactag ctgtcgtcgt
    29881 cttttttaaa tcgggtgact acgccactaa cgctcacaga tgaagaatct gcgaagctct
    29941 gttttatttt cactctagcg cttagggcaa aatgctacta tgctactgtc acaacctctt
    30001 tttacacacc actgctttgc agtttgtaca tatctgcata caaaccattt tgcgctagaa
    30061 gtgtttcatg attcccacgc tctactatat gacctttgtc taaaactaaa atcaattctg
    30121 catcccgaat cgtggacagc cgatgggcaa tcgctatcgt tgtccgtcct tgtcgcattt
    30181 ttgccaatcc ttcttggatc aatccttcgg tttcagtatc gatatttgct gttgcttcat
    30241 ccaatactag tattttagga tctgtcacga ttgttcgcgc aaaagtgatc agctgtcgtt
    30301 gtccactaga atagctggca ccgtgttcga tcacttttga gtgatatcct tttggtaatt
    30361 catcgatgaa cttgtttgct tgaacaaact cagctgcatg gcgaatctgt tcgtctgtga
    30421 tagctggatt catcatccgg atatttcctg caatatctcc ataaaacaag aaggcatctt
    30481 gcaagaccaa tcccattttc ttacgtaact cttccattgg atattctcga atgtcatgat
    30541 catcaatcaa gatttggcct tcataaaatt cgtaaaaccg catcaatacg ttgatgatcg
    30601 agctttttcc gcttccggtg tgccccacta atgcaacggt ctcacccgga ttagcgacaa
    30661 aagagatatt gttcaataca ttgtgctctc catcataaga aaagcttaca ttgcgaaact
    30721 cgatcttacc tgaagtaatc gcttgatctg ctccaggatt ttgttgcggt gcatattctt
    30781 ctgtatccat gattttcaaa atacgactac cggcaaccat cccatctgta aaggtgctca
    30841 aaaagtccat catttgagac attggattaa aaaattcctg gacataggtc gtaaatgcat
    30901 agatcagtcc aacttctacg ggagaatgca acgcatcgat cccaaacaaa gtcaacgcca
    30961 acgcaatcgc caatgcatag agaaaattga tgattggact taacagaatc gaattcgttt
    31021 tgatcatcgc atagcgcgtg tctaaatact cgttgttcac ttgttcaaat tctttagcta
    31081 aacgagcttc ttggcgaaac tgctggataa tacgcatgcc ggaaatcgat tcattcaatt
    31141 tcgtgttcag ctgacttaat ttttcccgca tatttcggta aatacgagaa ctgaatttct
    31201 ggtaatacca gatgacgatt cctaaaatcg gcaagaacac caaagtccac aaggcaattt
    31261 ctccattgat ttgaaacatg gcaacgaaag aagaaacgac tgcaaagatc ccagtcaata
    31321 ccatcaaaaa gacataccaa aattcaaata gcgtttctgt gtcattcgtc actcgagaaa
    31381 cggtcgatcc tgccggtgtc tggtcaaaat aacgcatacc tagtgtatgc agcttttcaa
    31441 ataatcttac ccgaacatac tgataggtct tcaatgaagc catggaatac aaataccatt
    31501 ggaaaaacca aatgatactt ttgactatga ctccaaacaa gtacacacca gcaaaaaaca
    31561 aaataacttg tgtcgtagct gactgtttag ccagatgatc atccataaaa gtttggatga
    31621 tccttggcaa taggacattg atcacagaca gggcaaacgc aaataagata gcaacgatga
    31681 acgttcttct aaaaggtttg gcgaacttca tcagtcgttt gatgatcttc ttctgttctt
    31741 taagtggaat cgattttgtc cattccgatt gataattttc atccatcagg cctcgcctcc
    31801 ttctatcttc gcttctaatt gctgtttttc ccacattcgt tcataccagc cacctaatgc
    31861 aagcagctcg tggtgggttc ctcgttcaat gattttccct tgatcgacaa caatgatttc
    31921 ttttgcatgc atcacgctgc ttaaacggtg agctgcaatg atggttgttt tctcttttct
    31981 tatttctttt aaattatgca agatagcttc ttctgttttg gcatctactg cagaaagtgc
    32041 atcatctaag atcagcaatt caggattttt gatcaatgct cgagcaatcg aaatcctctg
    32101 tttctgtccg ccagataaag agactccacg ttctccaact aaggtatcat aaccttttgg
    32161 caattccttg atttcattgt tgataaatgc taaagaagcc gcatgctcta cttcttcttc
    32221 tgaaagtttt ggattagaaa aacggatatt atctcgaatg gtcatcgaaa acaggaagtg
    32281 atcctgaggc acataaccga ttgatcctaa taaagcattc aatgaatatt ctttgatcga
    32341 acgtccgcca aattggattt tcccttgata atcatcgtat tcacgcatca gtaatttgat
    32401 gattgtcgtt tttcccgcac cagttttccc aacaatacct aatgtttctc cttcttggat
    32461 cgcaaatttg atttgttcta atgctggtga ttcttctttc ggatagttaa aagaggaaat
    32521 agctacattc agctcgcctt ttgccggggt ctgcactgca cccagttgtt ccacgatatg
    32581 cgtcttttca tgcaaaagtt cattgacgcg atcataagaa gcatttcctc tttccaatac
    32641 gttgaacaat cgaccaatcg caaacatcgg ccaaacaagc ataccgatat agctaataaa
    32701 tgagaccatc tgcccgattg agatcgtgcc ttccaagaca tactttccac ctaaaataat
    32761 tgttgcaaca taagacagac cgataatcaa ggtaatgaat ggatcaaata aggagtctaa
    32821 aaaattgact cgtttgtttt taataattgc atcttcaatc ttttcttcaa aatccgcgag
    32881 atcttctttt tcttgtccaa atgttttaat gactttgatc ccagtgatac tttcttgtgt
    32941 cttatcattg atactagaaa aagcttcttg cgaatcacga aaagcatcgt ggagttttga
    33001 ccccagcact cgtgaagtaa cggctagcaa aggcagagga atcagcgcaa tcagtgttaa
    33061 gcgccaatca ataaacaaaa tcatcgcaat gatcgttgtt ccgcccgtaa tcactgaatc
    33121 tgcaaaggtc aagattccag ctcctgctac attctggatt gcattcaaat cattcgttgc
    33181 atgagccatc aaatctccag tccgatattt ttggtaaaag agattgtcca tttctgtaaa
    33241 atgtttgaat agctgctgcc gaagttctct ctccaaccga gctgcacttc cccaaatatt
    33301 cgtccgccag atataacgaa aaagatattg taagaaagct gcaaggatca ataataagat
    33361 ccaccaggaa atgatcttta ttgataaatt cttatcggcg atctcatcaa tgatgatccc
    33421 aatcactttt ggaggaacta actgtaccaa ggccactgcg aatagggaga aaatcccaat
    33481 taaataatgt ttcttttcct gacgaaaaaa ccaacctaat tttctaaaaa tcgacatttt
    33541 actcctcctt tgtctttatc aagagcatag gattgtctgt gcttgtcttt tttgctgaat
    33601 agtctgaata gtctaacaaa ttacgaaata aaaaacggac tgaaaaaatc agcccgcaaa
    33661 aagtctttgg gtcagaacct attttttccc ttggttcttc atttgctgca tcatctggcg
    33721 aattttcttc tcagatggtt tttgccccat tgacatcatc attgatcgca acatgtcctc
    33781 attaacggga ggattttttt ctaaatagtc tttcatatat ttacgcgcta ggaaaaagcc
    33841 acctactgcg ccgattagca aagcaataat agcaataagt acgacaatac ctgttgatac
    33901 catataaatc tctcctttct atttccgaat tgctctcaat gatttatttt actaaagttt
    33961 attgaaaaaa gaaaggcgta ttctttagtt tcatgcaaaa aatagtggtt tttttagaga
    34021 agaacaaaat ttctttcatt aaccagcaag acacaccacg aaaaaaaatt aggcataccg
    34081 aatctttttc tttaattttt tctttctgag gtgtagaatg taatcgtaga taaataggaa
    34141 aggtggtttt ttcttgaata atcaatccaa caaacaattt cataagcatc gacttattga
    34201 acatacaaat ggtttatctt tagctgaagt aaatggaaca gtggatgtac caaagggaaa
    34261 aagttttttt aaaacacttc ttgcttactc tggaccaggt gcattagtag cagttggtta
    34321 tatggatcca ggtaactggt ccacttcgat cactggtgga caaaatttcc aatatttatt
    34381 gatgtcagtt attttgatgt ctagtctaat tgctatgctt ttacagtata tggcagcaaa
    34441 attaggaatc gtgacccaaa tggatttggc ccaagcaatc cgagcacgga caagtaagac
    34501 attgggtgtc attttatgga tcttgactga attagccatt atggcaacag acatcgcaga
    34561 agtcattggt gcagcaatcg ccctctactt actttttgat attccattgg tgattgccgt
    34621 ctttattact gtattagatg ttttcgttct cttactactg acaaaagtcg ggtttcgtaa
    34681 gatcgaagca ctggttgtct gcttaatttt cgtcatttta ttcgtttttg tctatcaagt
    34741 tggcttgtct aacccagatt ggcacagttt atttgctggc tttatcccta acagtcatac
    34801 attctcagac tcaccggtca tcggcggcga aactccgctg actggagctt tagggattat
    34861 tggtgcaaca gtcatgccgc ataacttata tcttcattca gcagtttcac aaactcggaa
    34921 gatcgatcac caaaacgaag aagatgtagc acaagctgta cgtttttcta cttgggattc
    34981 taatatccag ttgtcaatgg cttttctagt caatgcactg ctattgatca tgggtgtcgc
    35041 tgtcttcaaa accggttctg tccaagatcc ttcctttttt ggtctgtatg aagctttatc
    35101 tgatccatca gctatgagca atggtatttt gattgctgta gcaaaatctg gtatcttgtc
    35161 cactttattt gctgtagcac ttcttgcttc tggccagaac tctactatta ctggtacgtt
    35221 gaccggtcaa gtcatcatgg aaggattcat ccatatgcgt atcccgattt ggctaagaag
    35281 actggtcaca cgattgcttt ccgtgattcc tgtattgatt tgtgttttgt ttacaagtac
    35341 aaaaggaacg atcgaagaac atgccgcttt gaataactta atgaatgaat cccaggtgtt
    35401 cttggctttt gcgttgccat tttcgatgat tcctttactg atgatgacca acagccaagc
    35461 ggagatgggt agccgattta aaaattcatt catcgtaaaa cttcttggct ggttctctgt
    35521 gattagtcta acttacttga acttacgagg attaccagga cagatcgaaa gtttcttcgg
    35581 cgagcaagcc acttccgctc aactagcatt ggctgatcac atcgcttatg taatcattgt
    35641 tcttgtcctt ctcctgttgg catggacgat tggtgattta tataaaggaa acaaaaaata
    35701 tgaacagctt ttgcaggaaa cggtagaaga aatcgaataa aaactgattt ttccacacaa
    35761 agacccttga tagttaggtt aaaaatctag ctaccaaggg tctttttatt tagaagataa
    35821 agcttttttc agatttaatt tactgaaaac tagcatgctt gttactacga tgaattgcgt
    35881 taatggcacg cctgcaccag tgtagacact taaaaaatag tccttttttt ctggcgcata
    35941 agaacaaaac catgagacac cgaaaagaaa aaccaattgt gtcagtatta atccggtgac
    36001 cacaatcaaa gaggaactat aataggcacg atcagctttt gcaaatacgg ttcctagtat
    36061 cataagtaat atccctgcta ttgaagcaat cagcacggga atagccagaa cttggtaaat
    36121 ttttatgatg acattcgcga cttttgctga ttttgttaat tggaaattag gtgttctcgg
    36181 ttcattacta tcgatccaat tctccattag cggaaggttt aataattctt gagcctgttt
    36241 gacttgttct tgcgttccga aactataatt tcctccttga tggaaatctt tatatcgcca
    36301 tgcataagcg tatcctgatt gaaggaaatc gaatacgatt ggcagatcac tcatcgtctt
    36361 tccatctcct gatcccatca gatagaactc ttttttcttt ttgatggttc ccttgtgata
    36421 ggcttcttct aattcttggt tgactttttt ccaaaaatca ttggtttctt ttgcattgtt
    36481 ttggtaataa ccagattcgc ttaccacatc tcgcaatgcc caaaagatga tatctccggg
    36541 taattctttt acttttccac cttgagtcca cgcgtgagtc gtatataatt cgtcaatacg
    36601 ggtagaaaca gtatggaatg taggagatgc ggctaatgct ttatttagtg cagcatgtga
    36661 gacccatatc tcactgtttt ctttattcaa gttcgtttca tcatctattc ggatcagttg
    36721 tttcatcaaa cgtccaaatt ctccactgga acggtcattg atggttgcaa taccgtatgt
    36781 tgtctcattt ttatattcga tccactgata gacgatggaa aagccaacta taggaagtaa
    36841 aaacaatgaa agtttcagcc attcccgttt cgagagaaga aagagtcgtt tttttaactt
    36901 ctttacttct ttgaaagaaa agtcttgccc tagcaagatc tggacgaaac tgatacccaa
    36961 agctactaca gtaaaaggaa gaagccagat agaatcttcg cgaatgaacc aaaagaaagg
    37021 aaaagcgaca gaaagcccta aaaaccatgg cagcaatttt ctgctagaac cgtatttgcg
    37081 gtaataaata gctaataaac aagcgataat gatctcaaca gccggaatca cgagggcatt
    37141 acgatatgtt cgagtggaat attcatgtgt aaaactaatc ggagaaaaca aaaaataaaa
    37201 atagatactt aaaagaagat acttgttctt cacaatcggt ttaaacgcga gtgcaaccaa
    37261 tatactggca aaaatactga acatgccgaa caaaactgca taagaaagat gcagtttatg
    37321 tgcaagtact aaaaataagc tgtaggagat tcctttggaa agtgttgtag tcgtatactc
    37381 ccctagccat ttaccatcca aaatattttg agcatagtga atcagcagct gatcatcata
    37441 ggtcgccgac atatctgttc catagattgc tttactatat aaatacaacc gaataattgt
    37501 aaataatacg gctatgccaa gaataaagta tgtatgtttc ttttttagtt ggtcgatcaa
    37561 ggggtttaaa atgttcagca tcttttcgtc ctttcaatca cgttttgcta cttgtttgtg
    37621 aaacttttcg ttttttactt ttctatcagt ataccctgtt tgtctgaatt taaaaagagg
    37681 gggcaaaagg agcatggcga aaacgaatgc aaaaagcact cctttgtttc ttaaacaaaa
    37741 aaggacaagg cagactgccc aatccttttt tgttgctgtc aggtagaaaa cttaaagtgt
    37801 gacttcttct gctaataact cagggaacac ttcgggatcg aatgcttgcg ctttgaacat
    37861 ggaaatttct tttccatatg gtgcgtaagc tttcttttta tctttaccga catatggggt
    37921 ttctaatatt ttcgaaatgt tgtcaaacat tggatcatgg acgattttgt tcaatgtgtc
    37981 aaatccaatt gttccaaaac cgatattggc atgccgatct ttatgagaac cttgtggatt
    38041 tttagaatca ttgatatgca gtactttaag ccgatctaat ccgatgacct tatcaaactc
    38101 ttccattaca cctgaaaaat cttcttttat attatatccg gcatcattcg tatgacatgt
    38161 atcaaaagta acggataatt tttcattcaa ttttactcca tcgatgattt gcgctaattc
    38221 ctcaaatgtt cgaccgattt ccgtaccttt ccctgccatc gtctccaaag cgatctgtgc
    38281 cgtctgttct tttgtcaaaa cttcattcaa cccctcaata attctagcaa tcccagcttc
    38341 tgcccctgct cctacatgag cgcctgggtg aagggtgatt tgagtagcac ctaaagcttc
    38401 cgcacggaca atctcttctc tcagaaattg tacagcaaat ggaaacattt ctggtttgat
    38461 cgtatttccc aaattaatga tataaggcgc atgaacgacg atatttgtta gtccgttttc
    38521 tgccataaat gcttttccgg catcaatatt catctcttcg atagcttttc ttctagtatt
    38581 ttgaggagcg cctgtataga tcataaaggt cgaagatccg taactggctg cttcttctgc
    38641 cgcgcctaac agcattttct ttccgctcat gctgacatgt gagcctaata acatggatgg
    38701 attcctcctt gaaaggcaat tgtttcgaac aattcctgta tattgataag atgatgtaca
    38761 aagaaaatgg tttgtttttc tttgccctgt tctccatagt aactgaaact ttccctaaag
    38821 aaaagcttaa gaactctttc ataaaaaaga tcatttcctc acgttttatc tcattcgaaa
    38881 aagaaattcc tgtcaaaaac agccttgtct gtttttttgt cctattattt gctataatta
    38941 gcgaaataaa accattagtt gaggtgaccc ttttgccacg caaaaatact cgaaaaaaac
    39001 agaatagaag aaaaaaagaa aaatggtttg ttcctaagat tatttttaga gtgtttcaat
    39061 cattgactgt ttttattact gttcttcttt tgttgttcgc agctctaggt atcggaatag
    39121 gtgctggcta ctttgcctat cttgtcgaag acacacaatt gccgactaaa aaagaactgc
    39181 agacagaact aggaaacatt acggaaacgt caaagattgt ttatgcagat aatacagaga
    39241 tatcaaaaat ccagacagat ctgatgcgga cgactatttc atctgataag atttcgcctt
    39301 tattgaaaac cgcgattatc agtacagaag atgaatactt cgataaacat caaggttatg
    39361 tccctaaagc tgttttacga gcactagttt cagaagcgac tggcatcggc tcgtctggag
    39421 gatccacttt gacacagcaa ttagtcaaac aacagatcct aaccgatgaa acaacattca
    39481 aacgtaaagc aaacgagatc gttctttctg cacaagtaga aaaacacttt tcaaaaaatg
    39541 agatcattac tacgtatttg aatgtctcac catttggtcg gaataataag gggcaaaata
    39601 ttgctggagt ccaggaagct gctagaggaa tctttggtgt agatgcaaag gatgtgactc
    39661 tgccacaagc tgcttatatc gctggtttgc ctcaaagccc tatcacgtat agtccataca
    39721 cgaatacagg cgcgctaaaa gaagatcttt ctgccggtct tgccagaaaa gattttgttt
    39781 tattcagtat gtaccgtgaa ggacaaatta cgaaagaaca atacgaagaa gcgaaagcct
    39841 atgatcttac aaaagacttt ctcccacaac aaatcgcgga acagactgac agagaattct
    39901 tgtattatac cgtgatgaac gaagcaacaa ggattattgc acaacaattg gcagaaaaag
    39961 ataatgctga tatgtcagat tcatcggtca gtgaagctta ttatcaaaaa gcacagcaaa
    40021 cgatccaaaa caaaggatat acgatccatt cgacgatcga taaagatatc tatgctgcat
    40081 tacaaaccgg cgtagaaaat tatggctaca tgttagatga tggtgctggt agccaagtgg
    40141 agactggaaa tgtattgatg gataacagaa ccggacgtat ctatggcttc gtgggtggtc
    40201 ggaattacag ccagaatcaa aataaccacg cctttgatac agaacgtcaa gctggttcat
    40261 ccatcaaacc ggttcttgtt tacggtcctg caatcgatat gggacttgtc ggatcagaat
    40321 cccgtgtatc agattatgca acgacttggc aagaaggaaa aaatgctggt gagaaaatcg
    40381 tcaatgcgac caatgaaggg tcgaacacct tccaaaccgt ccgtgagtca ctggaatggt
    40441 ccaacaatat cccagcttat cacttatatc aagatgtgtt gaattccggt ggatcgaaac
    40501 aatacgctta cgagcactac ttggcgaaga tgaactaccc agcaaatgat aattggggcg
    40561 ttgaatcggc tcctttaggg acagtagatg taacgacgct tcaacagaca aatggattcc
    40621 aggccttagc aaatggtgga gtctacgaag aaggctatat cattgattcc atcacagata
    40681 atgcaggtaa tgtgatttat aaacatgaaa gtaattctgt ccgtatttat tccgaagcaa
    40741 cggcatcgat catgaatgac atgatgcgta gtgtgatcaa tgaaaaaatc actacaccat
    40801 tcaaagatgt catctcaagc ttaaacggaa atctaggaaa tgcagattgg gtcggaaaaa
    40861 ctggttctac caatgaatac cgagattcat ggctgatcgt ctcaactcca tctatcacta
    40921 tcagtagttg ggcaggacac gatgacaata ctggaatgga cagccaagca agaatacgtt
    40981 cggctaatta tcttgccaat ctaatcaatc aagcttacca agcaaaccct gagattttcg
    41041 gcactgatga gaaatttgac ttatcatccg atgtgatgaa gaaaaaagta tctgggttca
    41101 caggacaaac agctggaaaa gttactgtca ataaaaaatc catccaaacg ccaggaaaaa
    41161 cagtggagtc actttgggca aaaaatggtc cgaagaaaag cgcctttaaa ttcggcgtag
    41221 gcggtactga tgaaaactat acagattact ggaacaccgc ttcagcttat gctagagcga
    41281 atccagagac caaagaatca gacgataaag atgattgatc taatagatta atcagaatta
    41341 aagggagctg cgacaaaaat cttgtcacag ctcccttttt ccgaataaat ggtgttcaaa
    41401 agcttacatc tacgatatta agacaaattt atgtaaaaat gtgaaaaac
//
LOCUS       DACEMK010000011        41205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEMK010000011 DACEMK010000000
VERSION     DACEMK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.011"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..328)
                     /locus_tag="IW715_000506"
     CDS             complement(26..328)
                     /locus_tag="IW715_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1021130.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IW715_000507"
     CDS             complement(353..2446)
                     /locus_tag="IW715_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1021131.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IW715_000508"
     CDS             complement(2935..3699)
                     /locus_tag="IW715_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1021132.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW715_000509"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW715_000509"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1021133.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IW715_000510"
     CDS             complement(4704..5780)
                     /locus_tag="IW715_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1021134.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IW715_000511"
     CDS             complement(5804..6397)
                     /locus_tag="IW715_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1021135.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW715_000512"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW715_000512"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1021136.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IW715_000513"
     CDS             complement(8067..8366)
                     /locus_tag="IW715_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1021137.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW715_000514"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW715_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1021138.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IW715_000515"
     CDS             complement(10121..11032)
                     /locus_tag="IW715_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1021139.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IW715_000516"
     CDS             11152..12009
                     /locus_tag="IW715_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1021140.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IW715_000517"
     CDS             complement(12196..12558)
                     /locus_tag="IW715_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021141.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IW715_000518"
     CDS             12904..14343
                     /locus_tag="IW715_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1021142.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IW715_000519"
     CDS             complement(14350..16902)
                     /locus_tag="IW715_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1021143.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IW715_000520"
     CDS             complement(17016..18347)
                     /locus_tag="IW715_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1021144.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IW715_000521"
     CDS             complement(18313..19791)
                     /locus_tag="IW715_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1021145.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW715_000522"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW715_000522"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1021146.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IW715_000523"
     CDS             22401..23828
                     /locus_tag="IW715_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1021147.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IW715_000524"
     CDS             complement(23920..24285)
                     /locus_tag="IW715_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1021148.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IW715_000525"
     CDS             complement(24282..24647)
                     /locus_tag="IW715_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1021149.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IW715_000526"
     CDS             complement(24839..25087)
                     /locus_tag="IW715_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021150.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IW715_000527"
     CDS             25086..25214
                     /locus_tag="IW715_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1021151.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IW715_000528"
     CDS             25271..26965
                     /locus_tag="IW715_000528"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1021152.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="IW715_000529"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="IW715_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="IW715_000530"
     CDS             27170..27361
                     /locus_tag="IW715_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021153.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="IW715_000531"
     CDS             27739..28872
                     /locus_tag="IW715_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1021154.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="IW715_000532"
     CDS             28903..29223
                     /locus_tag="IW715_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021155.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="IW715_000533"
     CDS             complement(29252..29716)
                     /locus_tag="IW715_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1021156.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31100)
                     /locus_tag="IW715_000534"
     CDS             complement(29847..31100)
                     /locus_tag="IW715_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1021157.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31129..31446)
                     /locus_tag="IW715_000535"
     CDS             complement(31129..31446)
                     /locus_tag="IW715_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1021158.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31472..31792)
                     /locus_tag="IW715_000536"
     CDS             complement(31472..31792)
                     /locus_tag="IW715_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1021159.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32256..33671)
                     /locus_tag="IW715_000537"
     CDS             complement(32256..33671)
                     /locus_tag="IW715_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1021160.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33688..35004)
                     /locus_tag="IW715_000538"
     CDS             complement(33688..35004)
                     /locus_tag="IW715_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1021161.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35154..35852)
                     /locus_tag="IW715_000539"
     CDS             complement(35154..35852)
                     /locus_tag="IW715_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1021162.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36141..37130)
                     /locus_tag="IW715_000540"
     CDS             complement(36141..37130)
                     /locus_tag="IW715_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1021163.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37210..38043)
                     /gene="msrA"
                     /locus_tag="IW715_000541"
     CDS             complement(37210..38043)
                     /gene="msrA"
                     /locus_tag="IW715_000541"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1021164.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38218..39852)
                     /locus_tag="IW715_000542"
     CDS             complement(38218..39852)
                     /locus_tag="IW715_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021165.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39839..41173)
                     /locus_tag="IW715_000543"
     CDS             complement(39839..41173)
                     /locus_tag="IW715_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021166.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12502 a         8474 c         6958 g        13271 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
    30841 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
    30901 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
    30961 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
    31021 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
    31081 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
    31141 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
    31201 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
    31261 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
    31321 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
    31381 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
    31441 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
    31501 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
    31561 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
    31621 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
    31681 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
    31741 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
    31801 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
    31861 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
    31921 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
    31981 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
    32041 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
    32101 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
    32161 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
    32221 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
    32281 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
    32341 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    32401 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    32461 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    32521 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    32581 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    32641 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    32701 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    32761 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    32821 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    32881 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    32941 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    33001 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    33061 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    33121 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    33181 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    33241 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    33301 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    33361 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    33421 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    33481 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    33541 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    33601 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    33661 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    33721 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    33781 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    33841 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    33901 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    33961 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    34021 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    34081 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    34141 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    34201 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    34261 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    34321 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    34381 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    34441 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    34501 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    34561 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    34621 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    34681 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    34741 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    34801 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    34861 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    34921 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    34981 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    35041 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    35101 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    35161 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    35221 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    35281 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    35341 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    35401 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    35461 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    35521 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    35581 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    35641 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    35701 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    35761 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    35821 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    35881 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    35941 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
    36001 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
    36061 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
    36121 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
    36181 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
    36241 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
    36301 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
    36361 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
    36421 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
    36481 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
    36541 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
    36601 attttttttg tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt
    36661 cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga
    36721 tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg gtcacaactt
    36781 gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct
    36841 gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc
    36901 ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg
    36961 gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa
    37021 tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc tgaatgaccg
    37081 gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa tttttctcct
    37141 cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact acattttaaa
    37201 gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt tttttactcg
    37261 ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt tcggttgttt
    37321 tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg gttcgatttt
    37381 tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg attgttccgc
    37441 taattctttt tgtcgttcgc cttgataaaa aatcactgga cgatattgct tccctctatc
    37501 ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg tcagcaactc
    37561 gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat gacctgtgta
    37621 accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa
    37681 aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga aacatccccc
    37741 tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg aaaagtcata
    37801 tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta catcaggcgt
    37861 cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt
    37921 aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc gttcccaatc
    37981 tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt gttcctcatc
    38041 catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata
    38101 aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa aaaagagaga
    38161 atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa agttaattta
    38221 ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg taacaagata
    38281 agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa ctcctttgac
    38341 tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt gatgatttat
    38401 ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc tcttctcatt
    38461 agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta cccattgact
    38521 gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag cgataggaga
    38581 ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg tttctttgtt
    38641 tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt tactagaaac
    38701 agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc ttgtcttatt
    38761 gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata ttatttgcaa
    38821 gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca gaagatacca
    38881 tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa tctttttttc
    38941 tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca gaccaactag
    39001 tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat cgaacacatt
    39061 agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt agagcatttg
    39121 ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg ccgcactaaa
    39181 taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa ccaaacgaat
    39241 aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat ggctgtacac
    39301 tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt ttccagagat
    39361 gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag cttcggcaat
    39421 tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga gacaactaaa
    39481 taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag ctacgagtgt
    39541 tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat tttttgtcat
    39601 ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat ttacaccata
    39661 tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg ataacccctt
    39721 gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg tatttttgaa
    39781 aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta ctactttttt
    39841 atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa tctttcaact
    39901 tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc agaatactga
    39961 tccataaagc catgagagca gttggagtat attttgttgt aatttctatc ttattttctc
    40021 cagcatttac atccacattc actaagccat gttttttata agagtcattt acgactttac
    40081 cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact ttttgctttt
    40141 tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc cctttttccc
    40201 acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt atcgcttctg
    40261 ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg agtgtttctt
    40321 cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt tcttttgctg
    40381 ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat gaatattttt
    40441 tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga
    40501 tacctaagat acgccaagga aactggagca agcttatcgg tgttttttga aacaaaaacc
    40561 atggaaataa attagttacg catacccatg tagaaactac gccaacatag atgatttttt
    40621 cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc aatcctaata
    40681 accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta tctgaaatag
    40741 aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga taattcatct
    40801 gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa acagccttta
    40861 ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa aacaaacgaa
    40921 ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca tgagtataca
    40981 caagtagact tatactgaca gataaagcga tgactttttt ttctctattg aatatcaatc
    41041 cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct gcaattgcgg
    41101 atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa ctaaataaaa
    41161 aagcagcatc tatcttttta aaaaactttt ctccataata aaaac
//
LOCUS       DACEMK010000012        40677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEMK010000012 DACEMK010000000
VERSION     DACEMK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.012"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(7..963)
                     /locus_tag="IW715_000544"
     CDS             complement(7..963)
                     /locus_tag="IW715_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1021167.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1137..1697
                     /locus_tag="IW715_000545"
     CDS             1137..1697
                     /locus_tag="IW715_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021168.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1690..2514
                     /locus_tag="IW715_000546"
     CDS             1690..2514
                     /locus_tag="IW715_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1021169.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2524..3141
                     /locus_tag="IW715_000547"
     CDS             2524..3141
                     /locus_tag="IW715_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1021170.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3159..3842
                     /locus_tag="IW715_000548"
     CDS             3159..3842
                     /locus_tag="IW715_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1021171.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4525..5808
                     /locus_tag="IW715_000549"
     CDS             4525..5808
                     /locus_tag="IW715_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1021172.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            5824..6711
                     /locus_tag="IW715_000550"
     CDS             5824..6711
                     /locus_tag="IW715_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1021173.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6728..7564
                     /locus_tag="IW715_000551"
     CDS             6728..7564
                     /locus_tag="IW715_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1021174.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7567..8190
                     /locus_tag="IW715_000552"
     CDS             7567..8190
                     /locus_tag="IW715_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021175.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8187..9479
                     /locus_tag="IW715_000553"
     CDS             8187..9479
                     /locus_tag="IW715_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1021176.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9838..9935
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10051..10632
                     /locus_tag="IW715_000554"
     CDS             10051..10632
                     /locus_tag="IW715_000554"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1021177.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10635..11942
                     /locus_tag="IW715_000555"
     CDS             10635..11942
                     /locus_tag="IW715_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1021178.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12358..12846
                     /gene="purE"
                     /locus_tag="IW715_000556"
     CDS             12358..12846
                     /gene="purE"
                     /locus_tag="IW715_000556"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1021179.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12830..13963
                     /gene="purK"
                     /locus_tag="IW715_000557"
     CDS             12830..13963
                     /gene="purK"
                     /locus_tag="IW715_000557"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1021180.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13976..15271
                     /gene="purB"
                     /locus_tag="IW715_000558"
     CDS             13976..15271
                     /gene="purB"
                     /locus_tag="IW715_000558"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1021181.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15451..15828)
                     /locus_tag="IW715_000559"
     CDS             complement(15451..15828)
                     /locus_tag="IW715_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021182.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            16084..16812
                     /locus_tag="IW715_000560"
     CDS             16084..16812
                     /locus_tag="IW715_000560"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1021183.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16812..17066
                     /gene="purS"
                     /locus_tag="IW715_000561"
     CDS             16812..17066
                     /gene="purS"
                     /locus_tag="IW715_000561"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1021184.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            17068..17739
                     /gene="purQ"
                     /locus_tag="IW715_000562"
     CDS             17068..17739
                     /gene="purQ"
                     /locus_tag="IW715_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1021185.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17740..19962
                     /gene="purL"
                     /locus_tag="IW715_000563"
     CDS             17740..19962
                     /gene="purL"
                     /locus_tag="IW715_000563"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1021186.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19947..21386
                     /locus_tag="IW715_000564"
     CDS             19947..21386
                     /locus_tag="IW715_000564"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1021187.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21418..22461
                     /locus_tag="IW715_000565"
     CDS             21418..22461
                     /locus_tag="IW715_000565"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1021188.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22458..23036
                     /gene="purN"
                     /locus_tag="IW715_000566"
     CDS             22458..23036
                     /gene="purN"
                     /locus_tag="IW715_000566"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1021189.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            23033..24568
                     /gene="purH"
                     /locus_tag="IW715_000567"
     CDS             23033..24568
                     /gene="purH"
                     /locus_tag="IW715_000567"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1021190.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24584..25858
                     /gene="purD"
                     /locus_tag="IW715_000568"
     CDS             24584..25858
                     /gene="purD"
                     /locus_tag="IW715_000568"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1021191.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25980..26894
                     /locus_tag="IW715_000569"
     CDS             25980..26894
                     /locus_tag="IW715_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1021192.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDGLERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(27027..28592)
                     /locus_tag="IW715_000570"
     CDS             complement(27027..28592)
                     /locus_tag="IW715_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1021193.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28585..30036)
                     /locus_tag="IW715_000571"
     CDS             complement(28585..30036)
                     /locus_tag="IW715_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1021194.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(30050..31066)
                     /locus_tag="IW715_000572"
     CDS             complement(30050..31066)
                     /locus_tag="IW715_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021195.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31286..32374
                     /locus_tag="IW715_000573"
     CDS             31286..32374
                     /locus_tag="IW715_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021196.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32428..33954
                     /locus_tag="IW715_000574"
     CDS             32428..33954
                     /locus_tag="IW715_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021197.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            33956..35161
                     /locus_tag="IW715_000575"
     CDS             33956..35161
                     /locus_tag="IW715_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1021198.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            35418..36395
                     /gene="guaC"
                     /locus_tag="IW715_000576"
     CDS             35418..36395
                     /gene="guaC"
                     /locus_tag="IW715_000576"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1021199.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            36659..38083
                     /locus_tag="IW715_000577"
     CDS             36659..38083
                     /locus_tag="IW715_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1021200.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            38544..39653
                     /gene="pdhA"
                     /locus_tag="IW715_000578"
     CDS             38544..39653
                     /gene="pdhA"
                     /locus_tag="IW715_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1021201.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            39656..40633
                     /locus_tag="IW715_000579"
     CDS             39656..40633
                     /locus_tag="IW715_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1021202.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        12986 a         6937 c         9113 g        11641 t
ORIGIN      
        1 ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt
       61 tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat
      121 ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt
      181 aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc
      241 aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag
      301 ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg
      361 aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac
      421 agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt
      481 tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag
      541 ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat
      601 cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt
      661 tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa
      721 gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc
      781 gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt
      841 ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa
      901 gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt
      961 catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa
     1021 aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt
     1081 taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc
     1141 atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa
     1201 attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg
     1261 agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat
     1321 ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg
     1381 ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag
     1441 cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact
     1501 tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc
     1561 cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg
     1621 aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg
     1681 atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg
     1741 ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa
     1801 cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag
     1861 gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac
     1921 gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct
     1981 actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat
     2041 gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac
     2101 cggtctgtat catcccaaca acaagttttc gaacaaaatg tccaagatgt gatgagtcgt
     2161 caaggtgtat cagaagagga ggcgacaaag attactaaag aaacgattac ggagccagga
     2221 tacagcgaac accacacagg tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt
     2281 tatccttcta ctgtattgga tgcaagttac ggagatcagc ctggagctaa atggatcgca
     2341 gataatgctg cgcagtatgg atttatcgtt cgttatccaa aaggacacga agatataact
     2401 aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc tgagtatatc
     2461 accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa gtaggaattg
     2521 atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg
     2581 gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa tctgtcttct
     2641 ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg
     2701 cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag tatcatttta
     2761 ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa atacaataat
     2821 cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac aaaggaattt
     2881 gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc atgggaacag
     2941 tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc tgcgctgtat
     3001 gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca agatgcaggt
     3061 tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg
     3121 aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa tttacgccag
     3181 aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt ttagaagcaa
     3241 gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg gatattgcga
     3301 tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca cctcatccag
     3361 cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt gtaggtggtg
     3421 gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt
     3481 taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt tgtcgagtag
     3541 ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat gaaaagttag
     3601 cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa gcagtacgcc
     3661 attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca acggaagaaa
     3721 gtattgctga gaccattcaa gcaggagcaa atgccatcac atatacgcca ccaagtagcg
     3781 gagagctttt tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag aatcatgaat
     3841 aacaggtgag aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg tcttttgtac
     3901 tccagtacta tttgttcaga attcaaaaaa aagagaaatt tttttgtttt tttacctgta
     3961 atgtacataa gaagagttaa aaccttttgt tattattagc attctaacaa aaaaatgtct
     4021 tgatttattg aagcgctttc tatagaatga aaaacgaata aaaactaaaa aaggcacccg
     4081 acaatttagt agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact gaccaatcaa
     4141 gttgccatag tcaatgcttg tgcatcgacg tgtatattgt acctaatttc atacagaatt
     4201 actagttatt tttctgtttt ttagggaaga gcgcacacga agaagcatgc ttatttagct
     4261 atgtcaagga tattgggcag ttgtctgata tccttttttc tttgcgtaat tatcctccca
     4321 tcattgaagc aagaagtgtt tttagcgatg aaagaagaat cagcactatc taatttttct
     4381 tgaaagtatt gagttgttca tcctaggcag aacgataagg caagtcttgg tttttattcc
     4441 ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa atacagtata ttgaaaaata
     4501 acaatggaag tagaggtgca gagaatgaag aaaagaatga gaatcatctt agctttaagt
     4561 gttatgttat tcttcttgac ggcttgtggg caagaagatg acaaaaaaat cacgctgaac
     4621 atggcttggt ggggttcgca agtccgacac gatgcaacag taaaagtaat cgagctttat
     4681 gaagaaaaaa atccccatgt gacgatcgat tatgaatttt atgattatga aggatattac
     4741 acaaaattag gtacactagc agcatcagat gatatctggg acattttcca actgaacaat
     4801 caatttcctc aatacatcaa tcagattgaa ccattagatg aatatgtgga gcaagggata
     4861 attgatattt ctaatgcaga tgaatcctat ttagcaacga cgacttacga cgggcaatta
     4921 gttggactgt ctaacggggt gaattcgttt gcgattgctt acaataaaaa tatatttgaa
     4981 caattaaata tccctgaacc agcttcaaat tggacatggg atgaatttga acaaatcagc
     5041 tatcaaatca ctgaagaatt aggaatctat gctatttcaa gattcgaaga ttttatagct
     5101 ggctgtatta ttcaaatacc acaagttgag aaaggattga atttttataa tcgggaagat
     5161 ctatctaaca gcttgggatt tgaaaatcca gactatctaa cagatttttt ccagctgcgt
     5221 aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg gtggagctgc aagtacgacg
     5281 gatctgtctt ttcaggcggt tagggataga gaagcggcaa tggtgtttct atcaagtaac
     5341 cagttgacag aaatattgtc aggggctcct gaagatatgg agattcagtt gattaatcca
     5401 cctcgcagaa aagcagatgg agaatctggt atccctttac gttcttcgca aatgctgagt
     5461 atggcaaaaa gctcagccca taaagaagaa gctgctaagt ttattgattt ctttcaaaac
     5521 agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg taccaatcat gtcgaatatt
     5581 cgagaacagc tcgctcgtgc agatgatgca actttgcaag cgacatttga ttatttagaa
     5641 atgatttccg atatggacaa cggcgaagtg aatgtctttg atagtgcttt gaatccggaa
     5701 attcaagacc aatacaatct tttagttgaa aaagtcattt atgaagaagt aacagcagaa
     5761 gaagcagcag agagtcttta tgagtttgcg tcaaacattc taggatagca gcaggaggaa
     5821 ggcatgaaaa aagaaaaact aaaacggttc ttggatcaag acaatgtggt cggttacgta
     5881 tttgcttctc cattcatcct aggatttcta ttgataacag tgataccgat gatcttgtca
     5941 ctttattatt cgttcaccga ttatcgtttg ggaaatccag tctcatggat cggcttggat
     6001 aattatataa agatgctgag tgatgcccgt tttttgaact ccatccgagt cacttttctc
     6061 tatgtatttg catctgtacc tgtgaaactg atctttggtc tattgattgc ttatctttta
     6121 acgaggaata tcaaaggaac gactttcttt cgttctttgt attatgtgcc tagtttgatc
     6181 ggtggcagta tttctatcgc tctagtttgg cgggagttgt tttctaaaaa cgggttagtg
     6241 aatattttcc tggaacgatt aggcgcacct gcgctttcct ggtttagcga tcaaaaactg
     6301 gtcatgatcc ctcttgtttt gatgtctgct tggcaattcg gttcggcaat gatcatcttt
     6361 gctgcaggta tcaaagagat cccaggctcg tattatgaag cggcagagat cgatggagcg
     6421 aacaaatggc agacgtttta caagatcacg ttacctcttc tttcgccgat cattttgtat
     6481 aacttggtta tgcagacaat cgcggcgttt atgactttta cgcaggcatt tatcattacc
     6541 caaggtggtc cgaatgatgc cacgaacttt tatgctcttt atgtgtataa ccaagctttt
     6601 tcttttaatt ctatggggta tgctagtgct atttcgtgga tcatgctgtt gattatggga
     6661 gctgtcacct ttctgatttt caaatcatca aaaaaatggg tcgttgtgtg aatgagagga
     6721 ggaagaaatg aataatacag taacgaaatg gtttcaatac ggttttattt tgctattagg
     6781 tctagtcatg atttatccgg tcttatggat gatcgcaggt tcttttaaaa cggatcaaga
     6841 gattatgagt ggttcgctga atttgattcc agagactttt caatgggaaa attacgcaaa
     6901 aggatgggcg gggtttgctg gaatttcatt ttttactttc tttaggaatt cgtttgtcat
     6961 ctctgcagtt tcgacgatag ggacagttct gagctcgact tgtattgctt acgcattatc
     7021 gagaatcgat ttcaaaggaa aaagattctg gttcgtcgtc atgttagtaa cgatgatgat
     7081 tcctgcacaa gtgattttga ttccgcagtt tattatttac aatcggctga atttggttgg
     7141 gacgtatgtg ccgctgattt taccacattt ttttggacaa gcatttttca tttaccagat
     7201 catgcaattc atggtcagca tccccaaaga gttagatgaa tcagcgatta ttgacggatg
     7261 cagcaagtat agtgtcttta ccaaaattat ttttcctttg ctgaaacctt cgatcatcac
     7321 gacgatcatc atccagtttt attggaaatg ggacgacttc atgggaccat tgatttattt
     7381 aaacaagccg agatcttaca cggtatcgat tgccatcaaa ttatttgcag acgctggatc
     7441 tagtacgact tacgcgagta tgtttgcgat gtctacgttg tcgctgctac cggtttttct
     7501 gattttctta ttcttcaata aatatcttgt ccaggggatc agtacaagcg gtctgaaggg
     7561 ctgaatatga aaaagccaat agaagagatg tttctttaca agctcaacta ctatgtctgt
     7621 cggatttttt taaccagtct tatcagtaat gctcttttcg ctttagttct aggatgtttg
     7681 ttcactttag gattagtgct gtctgctcgt gttgtctatc tgatttattt tttgatgatg
     7741 tatccttctt attatctatc tgtccattac ttggcagaaa aaaaggtatg ttggaggaaa
     7801 ggaaaaatca aacggatgtg tttcatcatt acgttgtttc agctgtttgt cattattttt
     7861 gcagttgatt ggtatttatt agtgggaaag attggagctt ggtatctgat tccttttcta
     7921 ttgtgtctgt tgctttttgt atcccatagt ttttttagta cgctaattga tatctgcaaa
     7981 gaagataaag cagatagtct tgtttatttg atcaaaagga atatctggct gttgtcaaaa
     8041 aacgtcaagg ggtttctgtg gtatttatgc cttttgtatg gactgttctg gtgtatacag
     8101 catgattttt tactcactgc attactgtat ccagggctgc catttgtcac agtattttgc
     8161 ttaaaagaaa aggagaatga ctttaaatga agaaaaaata tgtacaagtc ggacttggtg
     8221 ggagagcgcg ttttttttat gaggcaattg cctctacgta tcatcaaact tgtgaactag
     8281 tcggtttttg tgatgtatct caaacaagga tggattatgc aaatcggata cttgtgaaca
     8341 agtatggaca gaaaaaagta aagacatgta tgccatcaca atttgagcaa ctactggatg
     8401 acgtacggcc ggctgaagtc attattacgt cagtagatcg aacgcatcat gattatatca
     8461 ttcgtgcaat gaaaaaagga tatgatgtta tttgcgaaaa acctctgact atcgatgagg
     8521 aaaaagctca agaaataata acggtccaaa aacagacggg aagaagcctg cgagtcactt
     8581 ttaattatcg ttatgcaccg tatcacacga agataaagga actgatagaa agtgatgtga
     8641 ttggtgatgt gttttctatc cacttcgaat ggctgttaaa tacgcaacac ggggcagatt
     8701 atttcagaag atggcatcga aataagcaca atagcggcgg gcttcttgtt cataaatcta
     8761 cccatcattt tgatctagtg aatttttggt tgaacagccg tccccaaaag gtatttgcat
     8821 tcggtggatt aaacttttat gggatgcata atgcggagaa acgcggagta gaaatatttt
     8881 acagtcgatg ctatggaagc gaactagcta aagctgatcc atttgctata gacctgtcag
     8941 ccaatgagga attgcgagaa atgtatttag aagcagaaaa agacagtgga tatattcgtg
     9001 accgcagtgt attttccgat gatatctcta ttgaagatac actaggcgta acggtcaaat
     9061 atgaaactgg tgcgattttg acgtattcgc taaacgcgta tatgccgtgg gaaggattca
     9121 atgtttcttt caatggcagc aaaggacgga tcgaaatgaa agtgatcgaa aagtcttaca
     9181 tcaatgcagg tggagataaa aaggatgaag gagcagccgt tcttcagtcc atccatgtct
     9241 atccgatgtt cggtgagcca tatgaggtaa tcgtcgaaca agaagcgggc ggacatggcg
     9301 gaggagatcc gcgtttatta gatgatctat ttggtgaaga aaaagaagaa gatccgtgga
     9361 atcgggcagc aactcacgta gatggtattt tgtcgatttt gacaggaatc gcagcaaatc
     9421 attctattgc ttcaggaaaa gcagtagtga ttgacgagct tgtcagctta aaagaataag
     9481 aatagattta cggaaattac ttcccgtagt ttataaaatc ttgccttatt gccgataggc
     9541 tgtcttttga acactattta ttcggaaaaa ggggctatga catgcttttt gtcatgcctt
     9601 ctttttatgt gggctgcttt tttagatagg agaaaataaa aataactatt taaacccgaa
     9661 caaaaaggaa taatgcatta aaaatcatta gattttacaa aaaaataagt aaaccacgtt
     9721 gtcagtttca gaaagtagtg ttagactgtt ttcaatattt gaaacaacat tttttattca
     9781 aatatggcaa actgccttca cgtgcaaaac agttgcaaga aaatgaattt acactattat
     9841 tagttttaca tacctactta tatatcgtca taatatggat gacagtttct agccagtacc
     9901 gtaaatgctg gactataagt aaaagattgg ctatttcacc cttcttttct tatgataaag
     9961 tggagggtgt ttttctttaa cagaaacata gtaggttttt gcacaaaagc ggttatttcg
    10021 gtattaataa atgataaagg agcaaataaa gtgaaagaat tagttgaacg tattcaaaaa
    10081 gacggacgta tattaggcga gggcgtatta aaagtcgaca gcttcgtcac tcaccaagta
    10141 gaccctgtat tgatggaaca aatgggaaaa cggtttgcag aagtatttaa agaacaagga
    10201 atcacgaaag ttgtgaccat tgaagcttct gggatcgcac cagctttatt tgctgcaaga
    10261 gaattagatg ttccaatgat cttcgctaga aaagcaaaaa gtttgacgat ggatgaagaa
    10321 ttattgacag cttctgttta ttcttttacg aaacaagtga caagtacgat ttctatttct
    10381 cgtaaatttt tatcttcaga agacaaagta ttgattatcg acgatttctt ggcaaacgga
    10441 caagcggcaa aaggattgat cgaattatgc cagcaagctg gagcgcaagt agaaggaatc
    10501 gggatcgtca tcgaaaagtc tttccaagat ggacgtcagt tacttgaaga aatgggattg
    10561 agagtcgttt ctcttgcgcg tattgcttcc ttgacgaatg gacaagttga gtttcttgag
    10621 gaggacgcat aatcgtggaa aaagaaacac aaaatggaaa agctgcggtt ttagggctgc
    10681 agcatctgct agcgatgtat gcaggagcag tagcggtgcc tcttttgatc ggtacaggac
    10741 taggctttaa tcaagaacag atgacttatt tgatttcaat tgatatcttt atgtgtggat
    10801 tagcgacatt attgcaattg acagttaacc gatttttcgg aattggcttg cctgtcgttt
    10861 taggatgtgc gatccaagca gtagctccac tgatcttgat cggaaccaat gaaggagtag
    10921 gagctatcta cggatctatt atcgcttctg ggatctttgt tgtgttagtt tctggtgtct
    10981 tttctaaaat taaaaaacta ttccctccta tagtaacagg aacggttatc accgttattg
    11041 gtttgacttt gattcctgta gcaatcgaga aaatgggtgg aggtaatgca acagcagctg
    11101 attttggtga caaaacaaat ctgctacttg catttgtgac gattctccta attgtaggcg
    11161 tgcaaatgct ggcaaaaggg ttcattcgtt caatcgcggt attgattggt ttagtcggag
    11221 gaagcatctt agctgctttc atgggaatga ttgatctgag cgcgattgga gatgcgccgt
    11281 tgttccatgt tccacaaccc ttttattttg gtaaaccaac tttcgacgta tggtcgattt
    11341 tactaatgat catcatttcc atggtcagca tggtagaatc aacaggtgta tattttgcac
    11401 ttggtgacat tactgggaaa aatgttggag aagaagaatt gaaaaaggga tatcgtgcag
    11461 aaggacttgc cgttatctta ggcggtattt tcaatacatt cccttatact ggcttttctc
    11521 aaaatgtcgg attggttcaa ctttcgggaa tcaaaacaag aaaacccatc tatttctctg
    11581 cattcttctt gattgtttta ggattattcc ctaaaatcgg tgcgttagca caaatcatcc
    11641 ctgagccggt acttggtgga ggaatgttag tcatgttcgg gatggtggca gtccaaggaa
    11701 tccgtatgct gttggaagtt gatttcagca atgataaaaa tctattgatt gttgctgttt
    11761 ctgttggatt tgggctgggt ttcaatgtta cgccgacatt attcagtcaa atgccgcaaa
    11821 cattgcaaat gttcactgga aatggcatcg taatgagtag cattacggct atcatattga
    11881 atttgatctt taatggattg aaaaaagatg aagaaatatt ggaagaagcc ttcaagaagt
    11941 aatagagcaa aagaaaggtt gtgaaatcag cgttttgacc tgattgttag agaaacaaac
    12001 ggaaaaatag ttcctcgtat tttgccgttt gtttgagcta ataccgaggg tcagctgatt
    12061 gaacaccgtt gaaggaagag gtcgtgagcc tgccgttcat ctccgagtca caaggaacaa
    12121 tttcaaaaaa aagcttctca tgtttgttga atattgttcc ttgtgaccga ggagttggca
    12181 ggagaacacc gtttatccat aaaaaggaat gtgacctaac ttttgtcaca ttcctttttt
    12241 ttatctttaa taaacacgaa caataaaatg tttttctttg ttaacattcg aaatttcttt
    12301 gacatcaaaa aaagtgattg ttaaaatatg aatgtagaaa aactgggagg aaaaacaatg
    12361 acagcaaaaa tctctgtgat tatggggagt acttcagatt gggaaacaat gaaagaagca
    12421 tgtcaaatat tggatgaatt cggtgtgtct tatgaaaaaa aggtcgtttc tgctcatcga
    12481 accccagatt tgatgtttga atatgcacaa aacgctcgaa aaaaaggaat caaaatcatt
    12541 attgcaggag cagggggagc cgcgcatctg ccgggtatgg tcgcatcaaa aacaactttg
    12601 ccggttatcg gtgtaccggt ccaatcacga acattaaatg gtttggattc gcttctttcg
    12661 attgtccaaa tgcctggagg agtacctgta gctactacag caatcggaaa agcgggagca
    12721 attaatgctg gactacttgc gatacagatg ctttccatgt atgatttaga attggaggct
    12781 aaattggctg aaagacgttc aatgttggct aaaactgtta tagaaagtag tgatcaactt
    12841 ggttaaacct ttgttgcctg gttcaacgat cgggatcgtc ggtggcggtc aattaggtcg
    12901 tatgatggcg attagtgcaa aagaaatggg ctttaaagta ggtgtgctgg atcctgtgag
    12961 cgattgtcca gccgcacaag ttgcagattg gcacattgaa agtgtctatg atgacacctt
    13021 tgcgctgaaa gagcttgcca gaagaacaga tgtcattacc tatgaattcg aaaacgtcag
    13081 tatagaggcg ttaaatgcga ttttacccat gtcatttatt cctcaaggga cagacttgct
    13141 tgcgattacg caagaccgcc tgttagaaaa atcttttttg gaaacgaata acatcgtgat
    13201 tgctccttat gcaacgatcg tcagtccgac ggatatccaa gatgcgattg acgggatcgg
    13261 ctatccttgt gttttgaaaa ccactcgagg cggctatgat ggcaaggggc agtatgtttt
    13321 gaaaagtcgg gcggatctag ctcctgccat ggacctttta agagaaggaa cctgtgtttt
    13381 agaagcatgg atcccgtttg aaaaagagat ttcgatcatg gtggcaggaa atggtcaagg
    13441 agatttcacg acttttccgg tcgtggaaaa catccaccac aacaatattc ttcatgaaac
    13501 gattgctcca gccgcgattg atcaggatgt gatcgaggag gctgagcgta ttgcccgtgt
    13561 gattgcagaa gcagtgagcc tttcaggtgt tttaggggta gaaatgtttt taacaaaaac
    13621 aggcgggcta tatgtgaatg aattagctcc tcgtcctcac aattctggac attattctat
    13681 tgaagcttgt tctatgagtc agttcgatgc ccatattcga ggtatttgtg gttggccatt
    13741 aggagaaaaa gtacgtttga taagcaatgc agtaatggtc aatgttttgg gagaacaact
    13801 gctggaaagc tatcgcttga ttgagaataa gccagactgg caattccatt tctatggaaa
    13861 aaaacaagca aaaaaaggtc gcaagatggg acatatcact atcttgacag aagacatcca
    13921 gcaaacgctg gaagaaatca aacaaacgaa tatttgggat tagaggagaa aaaacatgtt
    13981 agaacgttat acacgccctg aaatggggaa tatctggacg gaagagaaca aataccgtgc
    14041 gtggctcgaa gtcgagattt tagcagatga agcttgggca gaacttggtg aaatcccgca
    14101 agaagatgtg aaaaaaattc gtgagcatgc atcatttgat atcgatcgga tcttggaaat
    14161 cgaaaaatta acacgccatg atgtcgttgc gtttacacga gcggtttctg aaacattggg
    14221 agaagaaaga aaatgggtcc attatggtct aacaagtacg gatgtcgtag atactgctta
    14281 tggctatctc atcaagcaag caaatgatat tttacgggaa gacttgaaac gttttacgga
    14341 aatcattggc gaaaaagcaa aagaacacaa atatacagtg atgatgggtc ggacacacgg
    14401 tgtccatgct gaaccaacta cttttggatt gaaacttgcc acatggtatt cagaaatgaa
    14461 acggaacatc gaacgatttg aacatgcggc aaaaggagta gaagcaggaa aaatctcagg
    14521 agcagtggga acgttcgcaa atatcccgcc atttattgaa gagtatgtat gtaaacaatt
    14581 ggggatccgt ccacaagaaa tttctactca agttctccct cgggatctac atgctgaata
    14641 tttctcagcg atggcgctta ttgctacaag tattgaacgt tttgcaacag agatacgcgg
    14701 attgcaaaaa tccgaaacac gtgaagtaga agaatttttt gcgaaaggtc aaaaaggttc
    14761 ttctgctatg ccgcacaaaa ggaatccgat cggttcagaa aatatggctg gtctagccag
    14821 agtggtgcgc ggacacatgg tcactgccta tgaaaatgtc acattatggc atgaaagaga
    14881 tatttctcat tcttcagcag aacgaatcat cttgcctgat accacgattt tattgaatta
    14941 catgttaaat cgttttggca atatcgttaa aaatctaacg gtctttcctg aaaacatgaa
    15001 gcggaatatg tctgcaacat ttggtttgat ttatagccag cgcgttatgc tgaaattgat
    15061 cgataaggga atgacaagag aagaagctta tgatcttgtt cagccaaaaa ctgcgtacgc
    15121 ttgggacaac caagtgcagt tccgcccgct gttagaagaa gatccaacca tcacatcggt
    15181 attatctaaa gaagaaatag acgatgcctt tgattatcat catcatttga aaaacgttga
    15241 cgtgatattc aaacgagtag gtttggaata aagaagagtg agttgcaaaa ttcaattaaa
    15301 atagaatagc aaaaataacc aaaaaagaag cagatccgtt agcggttctg cttcttttag
    15361 tttattatat tttcttatca ataataggaa cctgatttta aaagcggcta cctctttacc
    15421 gtgcctcttt acatacaaaa aatagaggaa ttaagcgtaa atggcttggc tattataact
    15481 aaaatgctgg ttatggagat gttgcagcaa gttttcgaga aaagcgtatt ccatctcttg
    15541 tccattgaat aagatctgga tacgataatg atttttgaaa cgcttctgtt catgaatctg
    15601 actgattgaa agttcgcctt tgtgttctgt ctgataaaga aaagtccttt tacggcattc
    15661 agcacagcgc ttggttgctc ggcggatttt tcggaatgta taaaaactag tatctttatt
    15721 gcataaataa acgtctttga tcgacagcaa atcattatat agacaggaac gttcttcagc
    15781 tgttaattct gataaaaaaa gctgaccttg ttttattgca tcttccatcg gcaagtgcct
    15841 cctcgaaccc attatccaaa tttttagtta attctaatat accatcatta agtataaatg
    15901 taaacgcttg ttgatggtat aagattttcg tttctgataa aacacgaaca ttaaaagaat
    15961 ctaatgcctt aaatgtacat atttttgttg atttataaaa agatgattgc taaaatttaa
    16021 agtgaaaaaa cgaacaaaaa acgatgtatt tgcttttaat gtaaataaaa aggagagacg
    16081 tacatggaga agaaagaact tttgtatgaa ggaaaagcca aaaaaatata tcttacttct
    16141 gatgagaact tgatttggat agaatattta gatcaagcaa ctgcgttgaa tggcgcgagg
    16201 aaagaccaga taagtggaaa aggagaattg aataatcaaa tcacaagtgc aattttccgt
    16261 tatctgactc aaagaggaat aaaaaatcat tttattgaac aattgtctaa aaccgaacaa
    16321 ttagcaaaaa aagtacaaat tatccctctg gaagtggtta tccgaaatat tgcagcagga
    16381 agtttctcaa aacgtctagg tgttcccgaa ggtaaagaat tcatcaaacc aatcattgaa
    16441 ttctactata aggatgacca gttggatgat ccatttatca atgaagagca tatctatttc
    16501 ttacaacttg caaagccgga agaaatcagt gagctgaaag cccaagcgtt ggctattaat
    16561 gaaattttga ccgaattatt tgctaaggtc ggtttggttt tagtagattt caagctagaa
    16621 tttggccggt cgaatggaca aattttgctt gcggatgaag taacaccgga tacttgccga
    16681 ttatgggatg aacgtacgaa tgaacatatg gacaaagacg tttaccgcag agatctaggt
    16741 gatttgatcc cagtctatca agaagtatat aaccgtctat cggaactaac tgaagaaaag
    16801 gagcgagtat gatgtatttc gtaaaagtat atgtaaccta taaagattca gtgttagatc
    16861 ctcaagggga ggcagtaaaa aaagcagtcc atcgtatggg atatgaaaca atcgaagata
    16921 tccgaatagg aaaatacttt gagatacaac ttgctgatgc agaagaacca atcgatacag
    16981 tgattgaaac gatttgtgac aagctgcttg caaatgtaaa tatggaaaca taccgatatg
    17041 aaatcaaaaa aatggaggaa gtataacatg aaatttgcgg tcatcgtttt tccaggttca
    17101 aattgtgacg cagatatgtt atgggcagtt cgcgagatca tgggcgctga tgccgaattt
    17161 gtacggcatg atgagtcctc tttagaagga tttgatgggg ttttactgcc gggaggattc
    17221 tcctatggtg actatttgag atgcggtgcg attgctcggt tttctacaat catggaagaa
    17281 gtgattcgtt ttgctgaaga agggaaacca gtattcggta catgcaatgg attccagata
    17341 ttgacagaag caggactttt gccaggtgta ctgagacgaa atgagtcttt gcattttatt
    17401 tgcaaaactg taccattgaa agtcaacccc aagacggcct ttacttcaga gtatgaagcc
    17461 gacgaaataa tctatcttcc agttgctcat ggggaaggaa attatttctg tgacgaagaa
    17521 acacttcaag aactaaaaaa aaataatcag attgccttta cttataatga caacatcaac
    17581 ggcagcgtgg aaaacattgc agggattacg aacaaagcag ggaatgtgtt aggaatgatg
    17641 cctcatccag aacgtgcagt agaagacttg ctaggatcag aagatgggaa acggttcttt
    17701 gcttccattt taaaaaactt cggaaaggtg accgtataga tgaaaacatt gaaagaaccg
    17761 acagctgaag agatcaagaa aaaaagaatc tatgccgaat ggggattgac tgatgaagaa
    17821 tatcgaatga tcgaagaaga tatcttagga agaatgccta attatacgga aactggactg
    17881 ttttctgtta tgtggagcga acattgttcc tataaaaatt ctaaaccggt tctacgcaaa
    17941 tttcctacta gtggacctca tgtgttgcaa ggccctggag aaggcgcagg catcgtcgac
    18001 atcggtgatg gactagcagt tgtcttcaaa gcagaaagtc acaatcatcc ttcagccgtg
    18061 gagccttatg aaggagctgc gacaggagtt ggcggaatca tcagagatat tttcagcatg
    18121 ggggctcgtc caattgcgtt gctagattca ttgaggttcg gcgaattaga taatccaaga
    18181 acgaaatatt tactagaaga agtagttgca ggtatcggcg gatacggcaa ttgtatcggc
    18241 ataccgactg taggcggcga aattgcattt gatccttgtt atgaaggaaa cccattagtc
    18301 aatgcaatgt gcgtaggttt gattgaacac aaacatatcc agaaagggca agccgctggc
    18361 gtcggtaatt cgatcatgta tgtaggtgca aaaacaggtc gcgacggtat ccacggagca
    18421 acttttgcct cagaagaatt taatcaagaa gaagaacagc aacgttctgc tgtacaagta
    18481 ggcgatcctt ttatggaaaa gctgctgctt gaagcgtgtt tggaattaat cttgcatcat
    18541 tcggattctc taataggtat acaggatatg ggagcagcag gacttgtttc atctagtgcg
    18601 gaaatggctt caaaagctgg ctcaggactt atattgacat tagatgaagt cccacaaaga
    18661 gaaactggga tgacacctta tgaaatgatg ttatcggaat cacaagaacg gatgttgatt
    18721 tgtgttaaat caggagagga agaaaaaatc caagaactat ttcagaagta tgatctagat
    18781 gcagtcacta tcggtaaagt aacagacgac gggcaatatc ggctatatca tcaaggagaa
    18841 gaagtagcaa atctgccagt cgatgcatta gctgaagatg caccagttta tcataaagaa
    18901 atgaaagaac cagctcgaat cgtagaattt cagcagttag ctccttatca gccagttatc
    18961 gaagaacctg gacaagtatt gcttgatctg ctgcagcaac cgacgattgc ttcaaaacgt
    19021 tcaatctacg aaacttatga ttcacaagta caaaccaata cagttgtccg tccaggaagc
    19081 gatgctgctg tgatgagggt tagagggacg aataaagcgt tagctatgac cacagactgc
    19141 aacgcgcgct atctgtattt ggatccaaaa atcggaggac aaatagcagt ggcagaagct
    19201 gctagaaata ttattgctag cggtggtaag cccttggcga tcaccgactg cctgaactat
    19261 ggttctcctg ataaaccaga agttttttgg gaattatcga cttcagctga cggaattgct
    19321 gctgcgtgtg aaacgttagg aactcctgtt atttcaggaa atgtctcttt atataatgaa
    19381 acggatggtc aagcaatcta tccaacacca atgattggta tggtcggatt gattgaggat
    19441 cataaacata tcactactca ggaattcaaa aaatcagggg atctaatcta tatattaggt
    19501 aagacgtttg cagattttga tggcagtgaa ttacaaaaga tgcagcttgg aagaatcgaa
    19561 ggtgtgatcc gaaactttga tctatccatt gaaaagagaa accaagagtt agtattgaca
    19621 gccatccaaa acggattgat tgaaagtgct catgactgtt cagaaggtgg cttggcaatt
    19681 gcactggcag aatcagcttt caaacatcag ctagggatat ctgtacagtt tgaattgtcc
    19741 tctgctcaat tatttgcaga aactcaatca agatttgtgt tgacagtcgc accggaaaat
    19801 aagacacgat tcgaagaaat gatgggagat gctgctgttt tagcaggtaa ggtgacggat
    19861 gaagcgataa tcgaaatttc agcaactgac ggtcagatca aaatagaaac tgcagtagca
    19921 agaaagtgct gggaggatgc aatcgtatgt cttatgaagt gaagagttta aatgaagaat
    19981 gcgggatctt cggtgtttgg ggtcatccag acgccgctag tgtggcatac ttcgggctcc
    20041 atagtcttca gcatcgagga caagaaggtg caggtatcgt gtccaatgat cgaggaaagc
    20101 ttaaggggca tcgagatttg gggctgcttt ctgaggtatt caaagatcaa agaaaattgc
    20161 agcagttaac aggagaagct gcaatcggac atgtacgtta tgcaacagca ggcaatggaa
    20221 gtgtggacaa tattcagcct tttctattca aatttcatga tcagtcgatc ggcttagcgc
    20281 ataatggtaa cttgatcaat gctcgttccc ttagaaaaga gttagaggaa aatggggcga
    20341 tcttccattc taactccgat acggagatat tgatgcattt gatccgacga agtgaagaaa
    20401 cagatttttt aaataaatta aaaagtgctt tgaatgaagt caaaggtggc tttgcctatt
    20461 tattaatgac agaaacggca atgattgccg ctttggaccc aaatggtttt cgtccattag
    20521 caatcggaca aatgaaaaac ggagcatacg tgattgcctc ggaaacttgt gcattagaag
    20581 ttattggggc acgttttata agagatgtcc agcctggtga gatagtgatc atcaatgacg
    20641 aaggaatcca gattgattgc tttacgaagg acacgcagct ttctatttgt tcgatggaat
    20701 atatctattt tgccagacca gattcaaaca tcgcagggat caatgtgcat actgcacgga
    20761 aaaatatggg acggaattta gcgaaagaat cgccagtaaa agcagatatg gtcgttggcg
    20821 tacctaattc ttctttgtca gccgcaagcg gttatgcgga agctagtgga atcccttatg
    20881 aaatcggatt agtaaaaaat caatacattg cccgaacatt tatccagcca acgcctgagc
    20941 taagagagca aggtgttcga atgaaattat ctgctgtacg aggagtagtg gaaggtaaaa
    21001 aggtgattat ggtcgatgat tcaattgttc ggggtacgac aagcagacgg atcgtccatc
    21061 ttttgaaaga agcaggggca aaagaagtcc atgtgaggat tgcttctcct cctttgaaat
    21121 atccatgttt ttacgggatt gatattcaaa cgcgtaaaga actcattgcg gcaaatcaca
    21181 cgattgatga aatcaaagaa tgtatcggtg cagattcttt aggtttttta agtgaagagg
    21241 gtctgatcga atcgatcggt ctggaaaaag atgctccgta ttcaggattg tgtatggctt
    21301 atttcaacgg agattatccg acgccgttat atgactatga agaaaaatat caggaatcac
    21361 taaaagaaaa agtatcgttc ttttaaaagc agaccaagaa gggagtatat gaattcaatg
    21421 gcaaatgcat acgcaaaagc tggcgtagat gtcgaagctg gatatgaagt agtagaacgg
    21481 atcaagaagc atgtcaaaaa aacggaacgt ttaggtatga tgggcgctat aggaggattc
    21541 ggcggttgtt ttgatttatc ggtctttcaa ttaaaagagc cagtccttgt ctcggggacc
    21601 gatggcgtgg ggacgaaatt agcagtggca atcgaagcag acaaacacga tacaatcggg
    21661 atcgactgcg tagcgatgtg tgtcaatgat atcttagcac aaggcgcaga gccgttatat
    21721 ttcttagact acttagcaac ggggaaaaat gatccgttac gtttggaaaa tgtagtaaaa
    21781 ggtgtagcag atggatgtgt tcaaggaaat atggcgctga ttggcggaga aacagctgaa
    21841 atgcctggta tgtactcagg taatgattat gatctagccg gatttgctgt agggatagct
    21901 gaaaaatctc aattattgac aggagaaacg atcaacccag gagatgtgtt gcttggaata
    21961 gcatctagcg ggattcattc aaatgggtac tctcttgttc gcaaaatttt ctttgagcag
    22021 cagcagtttt ctttatcatc aaaactgcca gaattgaaaa aaacacttgg cgaagaattg
    22081 cttgagccaa cgaaaatcta tgtttcttcc ttgcttccgc tgatcaaaga aaagaaaata
    22141 aaaggagctg cacatatcac tggtggaggg tttgtagaaa atatcccgcg aatgttacca
    22201 gaatctatgg ctgcacaaat caaacttggc tcatggccga ttcttccaat attcggctgt
    22261 ttacaaaaat atggtgaaat tcctgagatg gagatgtatg aaatcttcaa tatgggcatc
    22321 ggtatggtcc tggcagtcga agaatcgaaa gtaaaagaaa tagtacacga aattgagcaa
    22381 gctggcgaaa aagtttatga aatcgggaaa gtgatcgcca aagaaaaaca tcgtgtggtg
    22441 tttataggag aagaacgatg agaatcgctg tttttgcttc tggaaacgga agcaactttc
    22501 aagcgctagc cgattatcta tcgaaaaaag gattggaatc ttcgattgac tggttgtttt
    22561 gtgatcagcc tgaagcatat gttttgaaac gtgcaactgc gttgagtgta ccggcagatt
    22621 gtttttcacc taaagaattt gattcaaaaa aagagtatga agaagcaatt ttgcataaat
    22681 tgaaagaaaa aaagatcgat ttgatcgtat tagctggata tatgcgaatc attggaccgg
    22741 tattgctgaa aaattatgat aaaagaatca tcaatatcca tccgtctctt ttacctgcct
    22801 tccctggttt gcatggcata cgtgatgcct ttgaagctgg agtaaaagaa acgggtgtca
    22861 caattcatta tatcgaccaa ggtgtagata ctggaccaat catccggcag gaaaaagtga
    22921 gaattgaaca ggaggacacg ttcgattctt tagaagaaaa aatccataga gtagagcatc
    22981 gaatctatcc agaagtgatt agtgaaatta ttgaaaatgg aggctatctg aaatgacaag
    23041 agcattgatc agtgtatcag ataaaactgg gattgtagca ttcgcccaag gtttgattga
    23101 acaaggagta gaaatcattt caacaggagg aacaaagcat gcgttggata aagctgggat
    23161 caaaacgctt tcgatcgaag aagtgacagg gtttcctgaa atgatggatg gccgtgtcaa
    23221 aacacttcat cctaaaatcc atggtggtct tttaggaaga cgcgatctcc ctagccatat
    23281 ggaggcaatg gaaacggaaa atatccaacc aatcgacttt gtttgtgtca atttatatcc
    23341 tttcaaagaa acgattatga aaccagatgt cgaaatagca gaagctattg aaaacattga
    23401 tatcggtggt cctagtatgc tccgtagtgc agcaaaaaac catgaatttg tcacctctgt
    23461 tgttgatcca tctgactatg aagaagtact ttcagaatta cgtcagtatg ggcatacttc
    23521 actttctttc agacaaaaac tagcggcaaa agtctttcgt catactgcgg catatgatgc
    23581 actgatcgct caatacttaa cggattgggt ggaagaaaaa gaaccggaaa aattgacact
    23641 tacgtatgaa cgaaaacaaa cgcttcgtta cggtgaaaat agccatcaag ccgctgcctt
    23701 ttatcagtcc gctcttctgg aaaaatattc gatcgcagca gcaaaacaac tccatgggaa
    23761 agaactttct tataataata tccgagatgc agatgctgcg ctacggatca tgaaggaata
    23821 tgacgagcca acggtagtcg ccttgaaaca catgaatcct tgtggtattg ggacagcatc
    23881 tacgatttta gaagcgtggg aagcagcgta tgaagcagat cctgtttcaa tctttggtgg
    23941 gatcatcgtt ttgaatcgag aagtagatct gccaacagca gaacagatgc ataagctgtt
    24001 tctagaaatc atcattgccc cttcttatac acaagaagct ttggaattac tgcaaaaaaa
    24061 gaaaaacatc cgattactga cagtagactt tgcgcaggaa gaaaatcaaa atcgagaaga
    24121 aacggtctca gtcttaggtg gacttcttgt acaagatcaa gatctggcaa tagaaaatga
    24181 agaagagtgg aaagtcgtga caaaacgtaa gccgactcca gcagaagcaa aagcattagt
    24241 gtttgcttgg agagctgtca aacatgtgaa atctaatgcg atcgtactgg caaacgaacg
    24301 tcagacagta ggaatcggag ccgggcagat gaatcgagtc ggttcagtaa aaattgcgat
    24361 tgaacaagcg caagagaaaa tggaaggtgc agttttagct agcgatgctt attttccaat
    24421 ggatgattcg gttgaatatg cagccaaaca tggtataaaa gcaatcattc agccaggagg
    24481 aagcatcaaa gaccaagatt ctattgatat ggcgaataaa tacgatctag caatggtctt
    24541 tactgatgtt cggcatttta gacattagag aggagtcgga taaatgaaca ttttggtgat
    24601 cggtgcaggt ggtcgtgaac atgcaataag taaaaaactt ttagccagtc cgaaggtgga
    24661 tacggtgtat tgtgcgccag gaaatccggg gatgacaaaa gatggtatca attgtgtcgc
    24721 tatcagtgag cgagatcatt cagcgttaat ccattttgtt aagaaagagg ctattgattg
    24781 gagtatcgtt ggtcctgaag ttcctttgtt aaatgggatc gtcgatgatt ttatggcgga
    24841 aggattgaaa atcttcggtc cgacaaaagc agcagctctg atcgaaggtt cgaaaagttt
    24901 tgctaaacaa ttaatggata cttatcaaat cccgacagct aaatccagaa gttttgaatg
    24961 ttacgaaaaa gcagcagcgt atatcaaaga aaatggtgcg ccaatcgtag tgaaagcgga
    25021 cggcttagca gctgggaaag gtgtagtcgt ggctaccaca gtgaaggaag cgctggatgc
    25081 ggcagaacaa atgttgaacc agcatcgatt tggggatagt agcaaaaaaa tcgtcgtaga
    25141 agagtttttg gcaggagaag aattctccct tttggcgttt gtccgtaata ccaaggtgta
    25201 tccgatggtc atttcgcaag atcataagcg ggcatttgat aacgataaag ggccaaatac
    25261 aggcggaatg ggtgcttatt caccagtacc gcaaatttca aaaaagatga ttgaaaaagc
    25321 tgtggaagaa gttttaaaac cagcggcttt gggaatgaaa gaactaggac gctctttcac
    25381 gggtgtttta tatgcgggtt tgattgctac gaaggaagga ccaaaagtga tcgaattcaa
    25441 tgcgcgtttc ggtgatcctg aaacgcaagt cgttctttct cggttgaaga gtgactttgc
    25501 gcaagtaatc gatgatttat tagagaatcg tacagtagag ttaaagtggc agcaagaagg
    25561 atacaatgta ggcgttgtgg ttgcagctgc cggttacccc gaagaatatg agacaggaat
    25621 ggttctacca gattttctgg aagaagagct ttctgtttac tatgctggag tttcctcaaa
    25681 taacggcaaa ctagttggag caggaggacg tctttattta gtagaagcat acggtgagac
    25741 gatcaaagaa gcacagaaaa aaatttacac cgcgttaatg gataaaacga cagatggaac
    25801 attttatcgt acagatattg gagaaaaagc tgccaatagc gaactgatcg tgaagtaaat
    25861 cagctgcttc catttataaa aaatacaaca aacataatcg gaagcgccga ttttagtgaa
    25921 gccttttcat aaagagtgtg gtacactttt gttgtgtata taaataagga ggagttcaga
    25981 tgaaaaaaat tcaattaggt acttctgatc agaaagcagc tgcagtgatc ttaggctgta
    26041 tgcgtatcaa caatgcggaa gatccagtaa aagtgattga gacagcttat gaaaacggca
    26101 ttgatttttt tgaccatgca gatatttatg gaaacggtga atgtgaagaa atctttgcag
    26161 atgctctagc aaaaacatca atcaaacgag aagatctttt tattcaaaca aaatgtggaa
    26221 tcgttccagg tgtgatgttt gatttttcta aaaaacatat cattgaatct gtagaaaaaa
    26281 gtttgacccg tctgaaaatg gattatgtgg atgctttact tcttcatcgt ccagatacat
    26341 tggtagaacc agaggaagta gctgaagcgt ttgatggatt ggaacggtca ggaaaagttc
    26401 gttattttgg cgtcagcaat caaaaaccaa tgcaaatcga attgctgaaa aaatatgttc
    26461 gtcaaccatt agtagctaat cagctgcaat ttggcttgaa acatactgga atgatcgatc
    26521 aagggattca tgtcaatatg acagatgaag caagtatcga tcatgacggt agtgtacttg
    26581 attattcccg attgcatgat atgacgatcc aagcatggtc accataccag tatggatttt
    26641 ttgaaggtgt atttatcggg aacgaaaaat tccctgaatt aaaccaaaca ttagaagaac
    26701 tagcagctaa atatcaaacg acaccaactg gcttggcttc tgcttggatt ttacgccatc
    26761 cagccaatat gcaagtgatc gccggtacga tgaacgtagg gcgaatcaag gaaatcgctc
    26821 aagcttcaga aattaatatg tctcgggaag attggtacga attgtaccgt gctgctggaa
    26881 acattttacc ttaaagattg tcataagcag gtcaaaaggg ctataaatta aaaagacaga
    26941 ataacttgaa agctgttttc agcttcgaga tattctgtct ttttttattg caaatggaaa
    27001 gagtatcatt gtacggattt cgtttattag ttatttttgt ttttaaggaa aagctgtgtc
    27061 cgatattctt ttggagaaat cccttgtttt ttcttaaata aataactgaa atagttaggg
    27121 tcattataac caatttcaaa agcaatatct gaaatacgat agtctgattg ctgcaataat
    27181 ttttttgcta attcgattct ttgttctgtc agataatcga tgaatgtttg gttaaacgat
    27241 tgagaaaaaa tcgtactgaa atgagaggga cttaatgcaa cttgttgagc gacccaagtt
    27301 aaggacatat ctggatcggt aaaattttct ttgataaaat ccaatgcatg gttcaaaatt
    27361 ggctgatatt tgttccttga aggctgtgag ggcagagcag ttaagaaaga aatgaacata
    27421 taaactgtcg aacggtaaag cgagtcgtcg gcgctgattg ctaagcgttg actggtggat
    27481 gtgcaatgaa ccatttcttg cgggattttc ttttctttca ggcagacatg ctggagtagc
    27541 ttagtcaaga caaataaacg atataatcgt gttcgagttg gtgtgccttg tggtttcgtc
    27601 aagtgttcga tgtacttaga tacttcgttt ttcgataatg aagacaaatc aagtgagagg
    27661 tcaaagtaaa agacagaatc aactggaagt ttcgcttctt gaatcaaatc ctcatagctg
    27721 ataatttgtt ctttaaggga actggcggtt tcaagtatca tctgcgtgat ctcaaaagat
    27781 gcaggtaatt cgctgattct ttctgcgaca ggaccaatgg cgacagaaaa attatttttt
    27841 tgtccctttt ctaattcatg gaccaacgtt tgggctacct gataactggt ctcgatcaca
    27901 ttttcttcaa gagaatcaac tattaaaaat ttgatgaatc gagaggaaac agcagagaag
    27961 atcacttgtt gattctccct aaataaaaac ttcaaataat cactgagatg agcgtaatct
    28021 tcaaagtctg tactgacttg gtttgctgct aataatacgg tcattttttt ccctgctaaa
    28081 gagcgattga aagattcagc aagtttcaat gtttcactga tcgtaagtga gcctttgaac
    28141 aaaccgttca agaaatgatt ttttttcaat tcgaaaacaa gatcagaaga agtgtgcgtt
    28201 tctttttgct gcaaatctaa agcagttact gctttttcta ttgcttgttt caattcgcta
    28261 cttttaatcg gtttaagcag ataatcatct gcctgtacct ggattgctcc tttcatataa
    28321 tcgaaatcgt cgaatccact aataaatatg agacgagtcc aaggaagtag tttccttgct
    28381 tcttttgaaa aagtcaaccc gtccatgaaa ggcatttgga tatcagtcag aatgatgtcg
    28441 ggctgtaagt cgataattga agacagtgcc atctcaccat ctccagcttc gccgacataa
    28501 gtgattggat aataagaacc aaggacagtt atttgttccc gcaagttatt gcgaatgagg
    28561 tattcgtctt cgatgataaa aactttatac atgtttgttt cctccaattg ttttgggaga
    28621 tagagggatc atcagtgtga cagttgttcc ttctcggtat ttgctgtcaa tcgtgatagg
    28681 tttttgatca tttccgtaat aaagaaggag acgcttgttt acgttgtaaa gtccgtagcc
    28741 gatactgaaa tctgaatcga tccctttggc taactcttta cgtatttctg ccagtttttc
    28801 tgcagggatg ccaatcccgt tgtctataac tttgaattgg atcatctcat caattttgca
    28861 tgtggtaata cgaataaatc ctttgtgacg gacgtacttc gttccatggt atacagcatt
    28921 ttcaatcaat ggctgcaaga tcatttttaa aatagaaaca ggaaaaagtg tttctggtat
    28981 atcgatctta tagtcaagca tatggccgta tcttattttt aagatgacta gataatcatt
    29041 gatatggcta atttcttttt caagaggaat ccagtcttgt cctttgctta aagacgtacg
    29101 aaaaaagccg gaaagtgcat gggtcatagt cagagcttct tcaaagcagt cttgttcgat
    29161 caaagaaata atggcatcca aactgttata aataaaatgg ggagtaatct gagcttgcaa
    29221 tacacgaact tcactttgtg ctaaatggta ttgtttcaaa gcgttctccc gtaaagactg
    29281 gtttagatct tccgccattt tattcatgct agcggtaagt gtttgtaatt ccagtgtttc
    29341 cggcagggtt aaacgaaagc caaggttccc tgaagaaagt tgttctgaca tctcgatcag
    29401 ctgattgatc ggttcttgga cctttctctc taaaaagcga cgattctttt tcgcatagaa
    29461 aaaaatggta ctaagaactc caagttccac tattgttaat agaatgagcg agcggacaag
    29521 tgtcgtattt tttttgcttg ctaagtcgat ctccacacga acaaaatctt gtaagatatc
    29581 tgacagcagt tgagtgacag aatctacctg attgatgatc tcttcaatgg tatccataaa
    29641 atcttttgcc aagatattat tgatcagctc gttttgataa tcttccagtg tatcgatcgt
    29701 acgtagcgat acatctaacg tactaatttc tttaagtgta tttgtattat ttttgatctc
    29761 tcgaatatca ttcctaagtt cttctaggat attttcctca ttgaactcat ctacggttac
    29821 ttggccagaa atcaagtccc acatactttc caaaactttt tgatcaacct tcaaagcaat
    29881 tgcatctgct ttttggatat ttgcgatcgt ctgttgataa atcaaaagct gttgcgaata
    29941 taacagtatg ctcagtagca aagggataac agcaaataga agcatggctt tgtttgcttg
    30001 ggcaaaaatc tgttttatgc ttaaattttt tgtcatgata cttctccagt caataatttc
    30061 tggttgggat gttagctata ttctgctggg aaaagacagt ctcggaaata aaaatatctt
    30121 taggtaatgt ttttcctgtt tgaatatttc caaaataacg agtaacggta ttggctacat
    30181 accagccgac atcaggttta cactcgacta cacaattgac gattcctgag cgtagcttct
    30241 cgatcatatc ttcttgtcca tcaatagtta caatgatcaa atcttctcca ggtttgattg
    30301 atgtttcttt tatcgcagct aaagcaccgt gagtcatttc atcactatgg ctgtataata
    30361 catcgatttc ggataaatca tttgtttcta aatatcgttt catctggtct tttcctttca
    30421 tccgaataaa atcaccgaca attcggtgaa caatagtcat cggaggttta tggtcgatgg
    30481 ttccatcttt ttttagggtt ggtttacgat tgattgattc caaaaatcct tcggagcgta
    30541 gcttggtcgg tgtggagttc tctgaaccag caagttctag gattctgatt tcctgtttct
    30601 ttgaatttgt gaagtgattg ctgacaaata aacccgcccg atttccttct gctttgaaac
    30661 tggggccaat gtgggtgacg aaaagctcgg gatcattcgt ttgaatatgt ctgtcgacaa
    30721 gtatgacagg gatccctgct cgctttgctt cttttaatac agagtcccac ccggattctt
    30781 gcaaaggagt aaaaatgatc atatccactt tataggcaat gaacgtccgt atatcttgga
    30841 tttgccgttc ctgattcatg tagccatttc ggtagagaac ttggtaatct tctttttcaa
    30901 gcgcagtgat aattgattca gtatgctttt tccgccagct actctccgtt cctgattgcg
    30961 aaaaaccaac gactagtggg tcaggtgctt cctttgtatt aggtgtacac cccatcattt
    31021 ggtaagcgat tacaacaagg gctaaaaaaa ataaaacacg tttcacttta aatcactcca
    31081 tctggatttg ttagtactat cttatcagat aaaagggaaa atcaaagtca aaaggaatca
    31141 atcgaaaaaa atccaagaaa cttaaaaaaa tccaagacaa aaaaagtaca cctaaaaaat
    31201 ttaaagtaaa tcccataaaa aaatcaatgt taacgctttc tttattagct atactcggtg
    31261 tatcaattga aatgggggat aaaaaatgaa aaacaaattt agaaagattg ttgggactat
    31321 cgctattttg accgcaagca ttctattaag tgcgtgtggc aattcaggat ctgcagatga
    31381 tagtacgggg tatgtaggaa tcgctatgcc gaccaaatca gcagaaagat ggatcgctga
    31441 tgggaacaac atggtctcgg aattagaaaa actgggttat aaaacagatc ttcaatatgg
    31501 agaagataaa gtagaaaatc aagtagcaca aatagaaaat atgattacaa aaggggtaga
    31561 tacacttgtg attgcttcta tcgatggttc ggctttaact gatgtgttag ctaaagcaaa
    31621 agaagcagat atcaaggtga tcgcctatga tcgtttactg atgaattcag aaaatgtcga
    31681 ttattacgcg acttttgata attttggagt aggtgtatcg caagcagctt atattgaaga
    31741 gcacttagga ttgaaagaag gaaaaggacc attcacgatt gaattatttg gtggctcgcc
    31801 tgacgacaac aacgcactaa tcaattacaa cggagtgatg tctgttttac aaccatatat
    31861 ggacaacggt cagttggtcg tgccatctgg acagacgagt ttcagtcaaa tcgctacgct
    31921 tcgctgggat ggttctacgg cgcaagcaag gatggataat ctgttaagcg caaattatac
    31981 tgaccaaaca ttggatgctg ttttgtcgcc atatgatcca ataagtttag gaatcatttc
    32041 gtcattaaaa ggcgtggggt atgggagtga aagtaaacct ttacctgtga ttactggaca
    32101 agatgcgaca gttgctggcg ttaaatcgat cattgccggg gaacaaacac aaacgatctt
    32161 taaagacaca cgtatcttag caaaaaatac gattgaaatg atcaaagcaa tcagtgatgg
    32221 agaagaggtt ccggtcaatg atactgaaac ttatgataat ggggtgaaaa ctgtccctac
    32281 gtatttagca aatacagtat ctgtcgataa agataattat caagcagagc tgatcgacac
    32341 cgattattat aaagaatccg atctgaaaaa ttaagaggaa ccaaacaagg aactaaagag
    32401 atctaagtca gagagaggat aagaattatg gcggattgta ttttaaaaat ggcagatatc
    32461 accaaaaaat tttctggaat ccgagcgtta gattgcgtga atctgatgat tgaacgtggg
    32521 gaaatccatg cattgtgcgg agaaaacgga gcgggaaaat caacattgat gaatgtcttg
    32581 agcggcttgt atccatatgg ctcctatgac ggggaaatct tttatgatgg aaagctgtgt
    32641 caatttaaag atttgaaaga tagtgaagaa aaaggcatcg tcatcattca tcaagaatta
    32701 gcactaagtc cttatctttc aataaaagag aatatttttc tcggaaatga acaagcagaa
    32761 agaggcgtga ttgattggga actgacagaa aagaaaacat atgaactgct gaataaagtc
    32821 ggtttgtcta tcgacccgaa tcttctagta tcccagattg gcgtagggca acagcaacta
    32881 gttgaaatag ctaaagcatt atcaaaatcc gtccgtctgt tgattttgga tgagcctacc
    32941 gctgcattga atgaagaaga aagcgcgaat ttgctgagat tgatccgaga gttgaaagag
    33001 caaggtgtaa cctccatcat tatttcacat aagctgaatg aaatcgtgga tatcgcagat
    33061 cgaatcacca ttttacggga tggacagacg attgagacac tagaaaatga aatgatcagc
    33121 gaagagcata ttattcgcgg aatggtgggc agagatttag ttgatcgtta tcctgaaaga
    33181 aaaccggata ttggagaagt ttattttaaa gtagaagact ggacggtcta tcaccctctt
    33241 gatcacgagc gaatcataaa cgaccacatc aacttgcatt tgaaaaaagg agagattgta
    33301 ggaatagctg gactgatggg agcgggcaga actgaattcg ctatgagtct atttggacat
    33361 tcttatggaa gcaagatatc cggacgtata ttaaaggaag gggcagagat atcagttaag
    33421 accgttcctt cagccattgc aaacggactc gcttatgtct cggaggaccg aaaatcttta
    33481 ggactgaatc tgctgatgga tattcgggaa aacgtgacgc tgagcagttt ggataaaata
    33541 agtcaaaaag gagtattaga taaagaaaaa gaagtaactg aggcagaaaa tttccggaaa
    33601 aagatgcgta taaaaacaaa ctccatccat caaattgtca gtagtcttag tggtgggaat
    33661 caacaaaaag tcgttttatc caagtggctg atgacacaac ccgatattct ttttttggat
    33721 gagccaaccc gtgggataga tgttggggca aaatatgaga tctacacgat tatcgaagaa
    33781 atggcagcaa atggcaaagc tgtctgtctt atctcttctg aacttcctga aatattaggg
    33841 atgtgtgatc gaatctatac gatgaacgaa ggacgtatta caggagaagt ctcgcgagaa
    33901 gatgcagatc aggaagtctt gatgaaatta atgacgaaag aagaggtggg ttaagttgga
    33961 acaggcaaaa aatcttacgg ataaaaaaga gaaaaaacta aatttgaaaa gtagagcagc
    34021 tgatcttttt ggtcgctaca gcatggtgat cattttagcg ggactactta ttgcgtttca
    34081 attgatgacg gatggtattt tctggcgtcc attgaatata actaaccttg ttctgcaaaa
    34141 tagccatata ttagtactag ctgcagggat gttattagtt gtgctcttag gtcatgtgga
    34201 tttgtcggta gggtcagtga tggcgtttgt tggtgcaatt gcgggcatga tgatggtcaa
    34261 tcgccaaatg aacccgtgga tgacagttat cgcatgtctt gcagtaggcg ggctgatcgg
    34321 cgcatggcaa ggtttttggg tagcttatgc agggataccg gcgtttatcg tgactttagc
    34381 aggattgctg atgtttcgtg gactgacaca agttgttcta ggaggccaat ctctagcacc
    34441 ttttccagct gttttccaaa aaatgtctac gggttattta ccagacttaa gcgaaggaag
    34501 cagccttcac ttcttgacat tgattttagg aatgatcatc acgatcgtgt tagttgttgg
    34561 tcaatggcga acaagaaatc gtcggaaaaa tcatttattt gaagtggaat ctccaacagc
    34621 tttcttggta aaggcggtat ttacagcagt cgttgtacta ggaataactt atatctttgc
    34681 ctcttatcaa gggtatccga ttattttgat cattcttggt ttgatcgttg cagcatatgg
    34741 gtttttgaca aataaaacag tagcaggtcg acagatttat gcgacgggcg gaaatcgaaa
    34801 agcggctgaa ctatcaggga tcaaaacaaa gcgaatcacg ttctgggtat tcgtcaacat
    34861 gggagtcatg gcagctttag ctggattagt tttagctgct cgattgaatg cggcaacacc
    34921 tcaagcaggt acatcgatgg aactggatgc aatggcggca gtatattttg gtggtgcttc
    34981 gacttccggc ggcattggaa caatcatggg tgcgattgtc ggtggcttgg tcatgggtgt
    35041 attgaacaat ggcatgtcga ttttaggcgt cggggtagac tggcagcagg caatcaaagg
    35101 attgatcttg cttttagccg ttgtgctaga cttgtataat aagaaaaaac gaacaacgta
    35161 ggagaacgcc ggttattcag aaaaaagact gtgacaagac atttgtcaca gtcttttttt
    35221 cgctaaaagc gaacattcat tgtttcgttt gtatagacta aaaataatat atgttttttt
    35281 accattcgta taaattctct ttttgctctt gatttttttg cttaattgag ttaaattaca
    35341 tagggcgcta aaaaaagcct tgattaaatt aataatgttc gtgttttgct ggatgtgaac
    35401 atcaggagga taatgtaatg aaagtatttg attacgaaga tattcaatta attcctaata
    35461 aatgtatcgt aaatagccgt tctgaatgtg atacaacggt cacattaggt aaacatacat
    35521 tcaaaatgcc agtagtgcct gccaacatgc agacgattat tgatgaaaca atcgcagaat
    35581 tcttagcgga aaatggttat ttctatatca tgcatcggtt tgatgaagca gctcgaattc
    35641 cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc gatcagtgtt ggcgtcaaga
    35701 aagaggaata ttcctttata gagaagttgg cagaagaatc gctaaatcct gattatatca
    35761 ctatcgacat cgcgcatgga catgctaact cagttattga tatgattcag catattaaaa
    35821 aatatttgcc tgaaactttt gtgattgccg gtaatgttgg tacaccggaa gccgtccgtg
    35881 aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat tggtccagga aaagtatgta
    35941 ttacaaaaat caagacaggc tttgggactg gaggctggca actggcagct cttagatggt
    36001 gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg gatcagaaca catggcgata
    36061 ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat tggttcattg tttgccggtc
    36121 acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat cgtgtataaa gaatactttg
    36181 gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt tgaaggaaag aaaatctgga
    36241 tccaacacaa aggatctttg aaagatacac tagtggaaat gcaacaagac cttcaatcat
    36301 ctatttctta tgctggagga agagatctgg aagccattcg aaaagttgac tatgtcatcg
    36361 tgaaaaattc gatttttaat ggagacacaa tctaaaaaat aaataaaata ttaggatgtt
    36421 gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa acaaaaatag ttctttatat
    36481 gttttgtttg gctggaatta ttatcgcaga tacaagcttt tgaacatcat tattcagaat
    36541 aagggactgt gataagattt ttgtcacagt cccttatgtt ttgcaaaaaa agtttttctt
    36601 ttttctcgta aattcggtaa aatcaaaaga aaaaatagac aatagaaaag agaagcttat
    36661 gtgggaaaaa gtagagaaaa aacaaggacc gatttatcgt caaattatgg aacagattat
    36721 gagagaaatc gaaaacggaa ggatcaatcc tggaagtcaa cttgaatcag aacgaaaact
    36781 agggcaactt tttggtgtga atcgttccac cgttgtccat gcattggaag agcttaggga
    36841 gcttggggta ctaacgagca aacgtggcag cggatggttt gttaatcaga ctgaatgggg
    36901 gaaattcgct gttccaagaa tcgattggcg gcaaatgatc tctcctcgtt atgagcagac
    36961 ggactggtat gagcaaaaaa taagagaagc aaagcagatc caaaaaacat cgtttcttga
    37021 tttatacgct agcgagatgc cagatgagct tttgccgaat tttgagtttc cttcttattc
    37081 tcttgaagaa atcttagcgg aagaaaaaga aatggatctt ttaggctata ctccgttacg
    37141 taacaaaatc aaacggtatt tggaaagtaa atttgaattg gcattttccc ccaaccagct
    37201 tcttgtgact ggtggcggtc agcaagcaat ctttttgatt ttacaaacga ttctttcagc
    37261 tggagagggg atagctgtag agtcaccttc ttttttttac cgattacctc ttttcaaagc
    37321 ttcaggaacg cgtttatttg ggataccgat ggatgaagaa gggatcgact tagaaaaact
    37381 gaaagaatcg atccataaaa ataaattgaa agcagtgctt atcaacccga attttcaaaa
    37441 ccctacagga aaagtgatga gtcaaaaacg acgagaaaag ctagtagaat tgtgtcgaca
    37501 gtatcggctt ccaattattg aagacgatgt atttacggat ctttcatttc taaaccaaga
    37561 agaagtcctt cctatacgaa cgattgatcc ggataatgtg ttatacgtcg gttcgttatc
    37621 tagggtttta ggaaaaacaa cgaaaatcgg ttgggtgata ggacctgaga cttttatctc
    37681 gcaactagct caagcgcaag aaatgatgga gttttcgatg aatatcctta cacaaatcac
    37741 agtggctaat gtatttaatg aagcaattga tttgaaaatg caagatgtaa gaaaaaagtt
    37801 gaaagaaaaa agccgactga taagcgcttg gggagattca caatcgtttt tcaagcttca
    37861 ttcaatttta ggtggttact atgcctggat tacttgggat gggaaaaaac tagaccccga
    37921 actagcagaa aaagtagtag atatgggtct tggaattgca ccaagtctac tttttgggca
    37981 agatataaat ggaatcagaa tcaattacag taggctggat gaaaaccaaa taccgttgtt
    38041 ttattcaaag atgaaaattc tagaaaagtg gttaacagaa taatttcaca aagtaaatag
    38101 attaaagaat ataacagctt ggatttaaat ttcaagctgt ttcttttttt tcggaaaaag
    38161 aatacgatat aaagaaacta agaaaaaacc attatttgta cgtccgttca cgatgtgtaa
    38221 ataaaataaa tgtttgcaac aaaactgtat aacaccaacg tttttaaata gaaacagatg
    38281 gttgtaagtt tgattagaaa atagaaaaaa tgcagatgaa atataaaacg ctttccttat
    38341 tgtgatttta ttcactaatg ctgttaatat gcggattggc ataatttcct ttcatcgtga
    38401 aaatttgtgc ttttagcttg tatttctcat aaatccatga taatttatta atgaagacgg
    38461 ttaaatagtg atacagtttt attcactgtt tcaatcgcag aatgaagaga ttttcttata
    38521 gatgagagag gaatgtgtga caaatggcaa acaaaaaagc accgatcgac ttccaagcac
    38581 tgctagatga agtcaatgca gatttcccag tatatcaagc acttgatcaa gatggaaacg
    38641 tagttaacaa agatttagta cctgatttat ccgatgatga actcgttgaa ttaatgactc
    38701 atatggtatg gtctcgtgta ttagaccaac gttcaacagc gctgaaccgc caaggacgcc
    38761 taggcttttt tgcaccaact gctggacaag aggcaagtca gctggctagt gcttacgcat
    38821 ttgataaaga agatgttctt cttccaggtt atcgggatgt acctcaacta gtaaaacatg
    38881 gtttaccttt atctcaagct tttttatggt ctcgaggaca tgctgcaggt aattcttatc
    38941 cggaagaatt gaaagctcta ccaccacaaa ttatcatcgg tgcacaatat gtacaagcag
    39001 ccggtgttgc attaggtttg aagaaacgaa acaagaaaaa tgtcgtcttc acttatactg
    39061 gtgatggcgg ttcatcacaa ggtgattttt acgaagcaat caatttcgct ggcgcttacc
    39121 atgcaaatgc cgtattttat atccaaaaca acgggtatgc gatctctaca cctcgtgaaa
    39181 aacaaactgc agcaaaaaca cttgcacaaa aagctgtcgc agctgggatt cctggtattc
    39241 aagtcgatgg aatggatccg cttgcagtat atactgtatc taaaatggca cgggaatggg
    39301 cgatcagtgg aaatggacca gtcctaatcg aaacactgac ttatcgttat ggaccacata
    39361 cactatctgg tgacgatcca acccgctacc gtacaaaaga cacagatgac gattggcaga
    39421 aaaaagatcc attgatccgc ttccgtaaat atttgacgga aaaaggactt tggtctgaag
    39481 aaaaagaaga acaagtcatc gaagcaacta aagaagaaat caaagcagct attgctgaag
    39541 ccgacaaagt accaaaacaa aaggtttcag atttcttgaa aaacatgttc gaagtatctc
    39601 cacaatcaat caaagaacaa attgcaattt atgaagcgaa ggagtcgaaa taatcatggc
    39661 acaaaaaaca atgatccaag caatcacaga tgccttagca cttgaactag aaaatgacga
    39721 gaatgtcgtt gtttttggtg aagatgttgg gaaaaacgga ggagttttcc gggcaactga
    39781 aggattgcag gaaaagttcg gagaagaccg agtattcgat actcctttag ctgaatctgg
    39841 aatcgcagga ttgagtttcg gtcttgcgtt agaaggattt cgtccagttc ctgaaatcca
    39901 attctttggt tttattttcg aagcaatgga cgaagtagta gctcaaatgg ctcgtacaag
    39961 atatcgaatg agcggcacta gaaatctacc catcacaatt cgttcgccat ttggtggcgg
    40021 tgttcataca ccagaattgc attcggataa tttagaaggt ttgatcgctc agtctcctgg
    40081 tattcgagta gtgattcctt ccaaccctta tgatgcaaaa ggactattga tttcttctat
    40141 tagaagtaac gatccggttg ttttcctaga acacatgaaa ctatatcgct cattccgaga
    40201 agaagtgccg gatgaagctt acgaagttcc tttggataaa gcagcagtta ctcgtgaggg
    40261 aacagatgta tctattatca catacggagc aatggtgcgt gaagcaatca aagcagcaga
    40321 caatcttgca aaagaaaata tatctgttga aatcattgat cttcgtacag ttgcgccatt
    40381 agatgtagaa acgatcattc aatccgtgga aaaaactggt cgcgtagttg ttgtccaaga
    40441 agcacaacga caagcgggcg ttgccgcgca agtagtttct gagatctcag aacgcgctat
    40501 cctttcatta gaagccccaa tcggacgagt ttctgcacct gatacagtct tcccatttgg
    40561 acaagcagaa aatgtctggt tgccaaatgc aaaagatatc gaagacaaag taaaagaaat
    40621 cgcggaattt taagaaaaga agggacgaaa caaagtcatg gcttatcaat ttaaatt
//
LOCUS       DACEMK010000013        37818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEMK010000013 DACEMK010000000
VERSION     DACEMK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.013"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..1469)
                     /locus_tag="IW715_000580"
     CDS             complement(159..1469)
                     /locus_tag="IW715_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1021203.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IW715_000581"
     CDS             complement(1481..2899)
                     /locus_tag="IW715_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1021204.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IW715_000582"
     CDS             complement(3139..4707)
                     /locus_tag="IW715_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1021205.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IW715_000583"
     CDS             complement(4711..5121)
                     /locus_tag="IW715_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1021206.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IW715_000584"
     CDS             complement(5118..6041)
                     /locus_tag="IW715_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1021207.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IW715_000585"
     CDS             complement(6038..6916)
                     /locus_tag="IW715_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1021208.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IW715_000586"
     CDS             complement(6903..8240)
                     /locus_tag="IW715_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1021209.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IW715_000587"
     CDS             complement(8243..9346)
                     /locus_tag="IW715_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1021210.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IW715_000588"
     CDS             complement(9420..10928)
                     /locus_tag="IW715_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1021211.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IW715_000589"
     CDS             complement(10894..11586)
                     /locus_tag="IW715_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1021212.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IW715_000590"
     CDS             complement(11608..12582)
                     /locus_tag="IW715_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1021213.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IW715_000591"
     CDS             12882..13754
                     /locus_tag="IW715_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1021214.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IW715_000592"
     CDS             complement(14212..16410)
                     /locus_tag="IW715_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021215.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IW715_000593"
     CDS             16642..17634
                     /locus_tag="IW715_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1021216.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IW715_000594"
     CDS             17694..19115
                     /locus_tag="IW715_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1021217.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IW715_000595"
     CDS             19108..20607
                     /locus_tag="IW715_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1021218.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IW715_000596"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IW715_000596"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1021219.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IW715_000597"
     CDS             22199..22882
                     /locus_tag="IW715_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1021220.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IW715_000598"
     CDS             22884..23720
                     /locus_tag="IW715_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1021221.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IW715_000599"
     CDS             23731..25485
                     /locus_tag="IW715_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021222.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="IW715_000600"
     CDS             complement(25761..26102)
                     /locus_tag="IW715_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1021223.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="IW715_000601"
     CDS             complement(26170..28341)
                     /locus_tag="IW715_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1021224.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="IW715_000602"
     CDS             28552..29409
                     /locus_tag="IW715_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1021225.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="IW715_000603"
     CDS             complement(29480..30337)
                     /locus_tag="IW715_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1021226.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="IW715_000604"
     CDS             complement(30322..33024)
                     /locus_tag="IW715_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1021227.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="IW715_000605"
     CDS             complement(33037..34326)
                     /locus_tag="IW715_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1021228.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="IW715_000606"
     CDS             34476..35522
                     /locus_tag="IW715_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1021229.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="IW715_000607"
     CDS             35524..37677
                     /locus_tag="IW715_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1021230.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11174 a         7789 c         6725 g        12130 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatca
//
LOCUS       DACEMK010000014        37288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEMK010000014 DACEMK010000000
VERSION     DACEMK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.014"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW715_000608"
     CDS             107..970
                     /locus_tag="IW715_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1021231.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW715_000609"
     CDS             complement(1099..2721)
                     /locus_tag="IW715_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1021232.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW715_000610"
     CDS             2970..3491
                     /locus_tag="IW715_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021233.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW715_000611"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW715_000611"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1021234.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW715_000612"
     CDS             5572..6390
                     /locus_tag="IW715_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1021235.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW715_000613"
     CDS             complement(6493..6690)
                     /locus_tag="IW715_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021236.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW715_000614"
     CDS             6859..7437
                     /locus_tag="IW715_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1021237.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IW715_000615"
     CDS             complement(8482..8985)
                     /locus_tag="IW715_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1021238.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9343..10368
                     /locus_tag="IW715_000616"
     CDS             9343..10368
                     /locus_tag="IW715_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1021239.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10691..11569
                     /locus_tag="IW715_000617"
     CDS             10691..11569
                     /locus_tag="IW715_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021240.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12045..12161
                     /locus_tag="IW715_000618"
     CDS             12045..12161
                     /locus_tag="IW715_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1021241.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13089..13427
                     /locus_tag="IW715_000619"
     CDS             13089..13427
                     /locus_tag="IW715_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021242.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13521..13751
                     /locus_tag="IW715_000620"
     CDS             13521..13751
                     /locus_tag="IW715_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021243.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14151..14522
                     /locus_tag="IW715_000621"
     CDS             14151..14522
                     /locus_tag="IW715_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1021244.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14796..15488)
                     /locus_tag="IW715_000622"
     CDS             complement(14796..15488)
                     /locus_tag="IW715_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1021245.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15491..16276)
                     /locus_tag="IW715_000623"
     CDS             complement(15491..16276)
                     /locus_tag="IW715_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1021246.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16513..17913
                     /gene="lacG"
                     /locus_tag="IW715_000624"
     CDS             16513..17913
                     /gene="lacG"
                     /locus_tag="IW715_000624"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1021247.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17938..18255
                     /locus_tag="IW715_000625"
     CDS             17938..18255
                     /locus_tag="IW715_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1021248.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18268..19941
                     /locus_tag="IW715_000626"
     CDS             18268..19941
                     /locus_tag="IW715_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1021249.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19955..21586
                     /locus_tag="IW715_000627"
     CDS             19955..21586
                     /locus_tag="IW715_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1021250.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22077..23642)
                     /gene="guaA"
                     /locus_tag="IW715_000628"
     CDS             complement(22077..23642)
                     /gene="guaA"
                     /locus_tag="IW715_000628"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1021251.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23701..24624)
                     /locus_tag="IW715_000629"
     CDS             complement(23701..24624)
                     /locus_tag="IW715_000629"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1021252.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24820..25425
                     /locus_tag="IW715_000630"
     CDS             24820..25425
                     /locus_tag="IW715_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1021253.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25733..26749
                     /locus_tag="IW715_000631"
     CDS             25733..26749
                     /locus_tag="IW715_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1021254.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26890..28191
                     /locus_tag="IW715_000632"
     CDS             26890..28191
                     /locus_tag="IW715_000632"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1021255.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28237..28899
                     /gene="deoC"
                     /locus_tag="IW715_000633"
     CDS             28237..28899
                     /gene="deoC"
                     /locus_tag="IW715_000633"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1021256.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28920..29318
                     /locus_tag="IW715_000634"
     CDS             28920..29318
                     /locus_tag="IW715_000634"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1021257.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29384..30466
                     /locus_tag="IW715_000635"
     CDS             29384..30466
                     /locus_tag="IW715_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1021258.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30678..32240
                     /locus_tag="IW715_000636"
     CDS             30678..32240
                     /locus_tag="IW715_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021259.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32233..33366
                     /locus_tag="IW715_000637"
     CDS             32233..33366
                     /locus_tag="IW715_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021260.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33366..34322
                     /locus_tag="IW715_000638"
     CDS             33366..34322
                     /locus_tag="IW715_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021261.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34421..35587
                     /gene="deoB"
                     /locus_tag="IW715_000639"
     CDS             34421..35587
                     /gene="deoB"
                     /locus_tag="IW715_000639"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1021262.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35611..36429
                     /locus_tag="IW715_000640"
     CDS             35611..36429
                     /locus_tag="IW715_000640"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1021263.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36446..37153
                     /gene="deoD"
                     /locus_tag="IW715_000641"
     CDS             36446..37153
                     /gene="deoD"
                     /locus_tag="IW715_000641"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1021264.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11628 a         6509 c         7892 g        11259 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacact
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc tttttttgtt gggaatccgg tttctttaac
     9121 aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg
     9181 gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg
     9241 aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc
     9301 ttggttcagc tttttttcca attactagga aggagggata gattgttcaa gacttatttt
     9361 cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt
     9421 attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta
     9481 gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg
     9541 tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt
     9601 ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga
     9661 ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg
     9721 agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc
     9781 gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca
     9841 attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct
     9901 gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat
     9961 aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg
    10021 atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca
    10081 ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat
    10141 ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg
    10201 atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat
    10261 cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg
    10321 gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat
    10381 atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa
    10441 agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa
    10501 aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct
    10561 gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa
    10621 gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt
    10681 catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa
    10741 tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat
    10801 tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga
    10861 tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt
    10921 tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc
    10981 attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca
    11041 tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta
    11101 cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa
    11161 agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga
    11221 tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga
    11281 actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc
    11341 caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg
    11401 attattatca gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga
    11461 tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt
    11521 gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag tttagtactt
    11581 cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa
    11641 cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    11701 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    11761 gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac tagctataac
    11821 aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa cgtaataatg
    11881 aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt ttaacaagtt
    11941 gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt tcgatttgtg
    12001 ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa aaaacagata
    12061 tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga
    12121 agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga aacttaatta
    12181 aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg tgtaaggttt
    12241 ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta gaatctttct
    12301 tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa ccacaaagca
    12361 cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa cctcttattt
    12421 tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg aaacattttt
    12481 agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt catgaaatat
    12541 ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata agcagttacg
    12601 aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa aaaaatcagt
    12661 gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat
    12721 atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa taataagttt
    12781 tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag taataaagta
    12841 tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa ttaatggtta
    12901 aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg tgatactaga
    12961 ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa tgaaaaagaa
    13021 gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt agccaagtaa
    13081 cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt cgtgctacag
    13141 aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct agtgagccaa
    13201 ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt atggtggtaa
    13261 ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt gaaaaggtga
    13321 gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc attttaacgg
    13381 attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca ctagtgaaat
    13441 actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa tcgatcatca
    13501 aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt tattacgtga
    13561 atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt cgctactcaa
    13621 ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa actgtttttt
    13681 ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat tatgttagta
    13741 tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt tattgttttt
    13801 tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta tccagagtat
    13861 tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa attcagaagt
    13921 tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata atatttttga
    13981 tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt tggtttttaa
    14041 tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa gttggttacc
    14101 ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata atgagagatt
    14161 acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat gaattatctg
    14221 ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac tatattgata
    14281 tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata aaagctttta
    14341 tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta gtaggaaaat
    14401 taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct ttgtctatgt
    14461 tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg gaaatctttt
    14521 aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat gatttttaag
    14581 attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga tagagatgaa
    14641 cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt tcactaagga
    14701 tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat
    14761 ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga tgtggcagac
    14821 aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt tagctgtgcc
    14881 tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt tccgatcccc
    14941 gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca atcagccgga
    15001 gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt cccagggaaa
    15061 gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc gaaaatatca
    15121 aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg aatctgttgg
    15181 tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc gttttcattt
    15241 tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc gatctcagag
    15301 gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg accatgaata
    15361 ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat catatgcagc
    15421 caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc aaatataatc
    15481 cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt tgcagatcga
    15541 catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta atgagcagat
    15601 cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt ttattatcaa
    15661 cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct ccttcttcgg
    15721 tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat gcttttgtaa
    15781 actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg gtatttttcc
    15841 ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga atatcaaaaa
    15901 ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc gccaatagtt
    15961 cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag gaggctgctg
    16021 tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg tgagaaagtt
    16081 cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc agcttgcccg
    16141 atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct aattgattga
    16201 tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata gtaagcaatc
    16261 tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat ataaacggac
    16321 aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg tttgttacaa
    16381 tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt gttgacaaat
    16441 gtttcggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt atagatttga
    16501 ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc cacagcagcc
    16561 tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt
    16621 ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca tcaatatgca
    16681 aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc cattgcttgg
    16741 agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc tttttatcat
    16801 cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt
    16861 gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac aatagaagca
    16921 tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt gtggagtacc
    16981 tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt cccaccagga
    17041 atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc
    17101 cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt agttcactca
    17161 ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc ctttttagac
    17221 gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta ttcaactgaa
    17281 accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa
    17341 gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg gatcaatcac
    17401 taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca taacggtacg
    17461 ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat ttataaagaa
    17521 ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata tgacttgttg
    17581 ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga gaacgggatg
    17641 gggtataaag atcagtttga agatggcatt attatggatc agccgagaat cgactatttg
    17701 agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt gaaggggtat
    17761 tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa aaggtatggc
    17821 ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc ttactggtac
    17881 aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga agaaaagatg
    17941 aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg cgacgctcgc
    18001 agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca tgttgattcg
    18061 gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac aaaaatttta
    18121 gcggaagaag catctgggaa agagatggag atgggattca tttttatcca tgggcaggat
    18181 catttaatga ccactttatt gctgagagat ctgattcaag actttattgt actgtacaga
    18241 cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg
    18301 aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg tgacggattt
    18361 ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc tgcggttcct
    18421 gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa agtctataat
    18481 tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg cttggctgat
    18541 tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc
    18601 tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt ttcaacggaa
    18661 tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac agttaattta
    18721 tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt tcctggaacg
    18781 atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc ggccgtttta
    18841 attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat cattacggtg
    18901 ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat ctggggagcg
    18961 atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg
    19021 gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct
    19081 tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg aactggggca
    19141 acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt gaaggcagtt
    19201 gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct atttgcggca
    19261 cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt tgtaaacgtt
    19321 tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta cgttttgcct
    19381 tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat cttagctttt
    19441 gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt ttgcaaagca
    19501 tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa
    19561 ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt tatcagcgat
    19621 gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag tgcgatgtta
    19681 gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt
    19741 gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt agcaccgcaa
    19801 gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat caagctggtt
    19861 gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc tgttcagttt
    19921 attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt ggttgcgtaa
    19981 agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc
    20041 atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata tggaaaaatc
    20101 tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt taaaaaataa
    20161 tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta atggagaaag
    20221 ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac gagcaaaaaa
    20281 tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg atcctgggaa
    20341 acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata cggcattgta
    20401 cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc ctgatatggt
    20461 tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt cttggggaga
    20521 aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc aagctgttcg
    20581 agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg gcgatacagg
    20641 agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg atttaattgg
    20701 agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca atatgaatgc
    20761 gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg ggcatacaac
    20821 agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg cggggggata
    20881 tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc ggattcaggc
    20941 agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt acaacggaaa
    21001 tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca ctatgggaaa
    21061 cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc
    21121 gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg aaagcgatgg
    21181 accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg ctaagggtac
    21241 gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt ggcaggatca
    21301 agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa cttacacatt
    21361 gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata tcaaaaatta
    21421 tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa aaattacaat
    21481 caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca aggcttcagg
    21541 aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg cggatcgttt
    21601 cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca ggaatcgaac
    21661 ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt taccaaaacg
    21721 agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc aacgatttcg
    21781 taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa
    21841 gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat agaaaaaagt
    21901 tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga aacaaattaa
    21961 cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa tcaagcattt
    22021 tagctattga agaatgttga tttataggga ttccccctcc atattatgtt ggctctttat
    22081 tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg
    22141 gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca
    22201 aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa
    22261 gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc
    22321 cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg
    22381 cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga
    22441 ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca
    22501 cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct
    22561 ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg
    22621 ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt
    22681 tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt
    22741 tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt gactttgatg
    22801 atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt
    22861 agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga
    22921 accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca
    22981 acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct
    23041 tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga
    23101 cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca
    23161 atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag
    23221 tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga
    23281 tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa
    23341 ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct
    23401 aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg
    23461 ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg
    23521 ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac
    23581 tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc
    23641 aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg
    23701 ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat
    23761 atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt
    23821 ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata
    23881 ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca
    23941 tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac
    24001 gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc
    24061 aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac
    24121 aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc
    24181 catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg
    24241 atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg
    24301 agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc
    24361 gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa
    24421 gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat
    24481 atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc
    24541 ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg
    24601 gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt
    24661 ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa
    24721 tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga
    24781 tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct
    24841 gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc
    24901 tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt
    24961 gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg
    25021 ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa
    25081 atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa
    25141 atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat
    25201 gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg
    25261 acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa
    25321 caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc
    25381 acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag
    25441 tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt
    25501 gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag
    25561 agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag
    25621 tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc
    25681 ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct
    25741 gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt
    25801 gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa
    25861 tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac
    25921 catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt
    25981 agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca
    26041 agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct
    26101 ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat
    26161 gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa
    26221 ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat
    26281 cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg
    26341 tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt
    26401 gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc
    26461 tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg
    26521 caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata
    26581 catgattccg caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc
    26641 acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga
    26701 aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat
    26761 attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag
    26821 tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag
    26881 gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg
    26941 aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa
    27001 gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac
    27061 ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata
    27121 aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc
    27181 ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca
    27241 ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa
    27301 gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg
    27361 gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt
    27421 ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt
    27481 ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg
    27541 gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca
    27601 gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc
    27661 gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc
    27721 caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa
    27781 gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg
    27841 ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag
    27901 cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct
    27961 gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga
    28021 ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc
    28081 tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt
    28141 ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa
    28201 gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac
    28261 catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca
    28321 aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa
    28381 caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac
    28441 acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc
    28501 gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat
    28561 atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tgttgaaaca
    28621 tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct
    28681 gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt
    28741 ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac
    28801 gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga
    28861 gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca
    28921 tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg
    28981 ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc
    29041 aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt
    29101 ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca
    29161 cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc
    29221 ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg
    29281 cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg
    29341 actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt
    29401 atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa
    29461 cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat
    29521 cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga
    29581 aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc
    29641 ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac
    29701 gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac
    29761 aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc
    29821 aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga
    29881 aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc
    29941 tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt
    30001 gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat
    30061 gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt
    30121 ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga
    30181 ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt
    30241 gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga
    30301 cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct
    30361 aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat
    30421 catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag
    30481 ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta
    30541 tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc
    30601 aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt
    30661 taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca
    30721 aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa
    30781 ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg
    30841 gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa
    30901 gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg
    30961 atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc
    31021 ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag
    31081 tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt
    31141 taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga
    31201 cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt
    31261 cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag
    31321 ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat
    31381 tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc
    31441 ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag
    31501 ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg
    31561 atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg
    31621 gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac
    31681 atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat
    31741 tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc
    31801 tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa
    31861 caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta
    31921 tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac
    31981 tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca
    32041 aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg
    32101 ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg
    32161 aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg
    32221 gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt
    32281 tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc
    32341 atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa
    32401 acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca
    32461 gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc
    32521 tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc
    32581 ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt
    32641 ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca
    32701 tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg
    32761 atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc
    32821 gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag
    32881 aaaacgactt taggatttga aatccgttct gttggattga atccttttgc ttctgagtat
    32941 gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca
    33001 gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact
    33061 tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc
    33121 ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag
    33181 acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt
    33241 gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa
    33301 gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa
    33361 ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa
    33421 caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg
    33481 tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga
    33541 tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag
    33601 gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta
    33661 tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat
    33721 ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag
    33781 gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt
    33841 attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct
    33901 tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat
    33961 atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg
    34021 tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca
    34081 tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat
    34141 tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt
    34201 cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg
    34261 ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat
    34321 aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg
    34381 ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct
    34441 gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt
    34501 tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga
    34561 gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca
    34621 aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg
    34681 ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga
    34741 tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta
    34801 tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat
    34861 tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt
    34921 agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat
    34981 gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag
    35041 caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga
    35101 agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat
    35161 cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat
    35221 gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg
    35281 tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc
    35341 agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa
    35401 cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag
    35461 taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac
    35521 gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt
    35581 aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa
    35641 acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt
    35701 tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag
    35761 atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct
    35821 ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc
    35881 atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt
    35941 gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt
    36001 accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg
    36061 ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa
    36121 gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga
    36181 ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta
    36241 gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga
    36301 atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt
    36361 gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca
    36421 ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat
    36481 cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt
    36541 tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata
    36601 taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta
    36661 tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg
    36721 tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag
    36781 ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa
    36841 tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa
    36901 tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga
    36961 acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata
    37021 ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac
    37081 aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc
    37141 gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa
    37201 gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt
    37261 tgaacaccgt ttaccaagag gttgtgaa
//
LOCUS       DACEMK010000015        37162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEMK010000015 DACEMK010000000
VERSION     DACEMK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.015"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            308..1969
                     /locus_tag="IW715_000642"
     CDS             308..1969
                     /locus_tag="IW715_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1021265.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2039..2386)
                     /locus_tag="IW715_000643"
     CDS             complement(2039..2386)
                     /locus_tag="IW715_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1021266.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2470..3411
                     /locus_tag="IW715_000644"
     CDS             2470..3411
                     /locus_tag="IW715_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021267.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3412..4455
                     /locus_tag="IW715_000645"
     CDS             3412..4455
                     /locus_tag="IW715_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021268.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4461..5531
                     /locus_tag="IW715_000646"
     CDS             4461..5531
                     /locus_tag="IW715_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021269.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5521..6474
                     /locus_tag="IW715_000647"
     CDS             5521..6474
                     /locus_tag="IW715_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1021270.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6486..7496
                     /locus_tag="IW715_000648"
     CDS             6486..7496
                     /locus_tag="IW715_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1021271.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7781..8602)
                     /locus_tag="IW715_000649"
     CDS             complement(7781..8602)
                     /locus_tag="IW715_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1021272.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8699..8977)
                     /locus_tag="IW715_000650"
     CDS             complement(8699..8977)
                     /locus_tag="IW715_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021273.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9245..11254
                     /gene="metG"
                     /locus_tag="IW715_000651"
     CDS             9245..11254
                     /gene="metG"
                     /locus_tag="IW715_000651"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1021274.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11733..12320)
                     /locus_tag="IW715_000652"
     CDS             complement(11733..12320)
                     /locus_tag="IW715_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021275.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12495..13817
                     /locus_tag="IW715_000653"
     CDS             12495..13817
                     /locus_tag="IW715_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1021276.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13972..14748
                     /locus_tag="IW715_000654"
     CDS             13972..14748
                     /locus_tag="IW715_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1021277.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14741..15310
                     /gene="rnmV"
                     /locus_tag="IW715_000655"
     CDS             14741..15310
                     /gene="rnmV"
                     /locus_tag="IW715_000655"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1021278.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15303..16187
                     /gene="rsmA"
                     /locus_tag="IW715_000656"
     CDS             15303..16187
                     /gene="rsmA"
                     /locus_tag="IW715_000656"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1021279.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16638..17267
                     /locus_tag="IW715_000657"
     CDS             16638..17267
                     /locus_tag="IW715_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021280.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17391..18500)
                     /gene="ugpC"
                     /locus_tag="IW715_000658"
     CDS             complement(17391..18500)
                     /gene="ugpC"
                     /locus_tag="IW715_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1021281.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18709..19131
                     /gene="mgsA"
                     /locus_tag="IW715_000659"
     CDS             18709..19131
                     /gene="mgsA"
                     /locus_tag="IW715_000659"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1021282.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19454..19990
                     /locus_tag="IW715_000660"
     CDS             19454..19990
                     /locus_tag="IW715_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1021283.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20030..21793)
                     /locus_tag="IW715_000661"
     CDS             complement(20030..21793)
                     /locus_tag="IW715_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021284.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21796..23511)
                     /locus_tag="IW715_000662"
     CDS             complement(21796..23511)
                     /locus_tag="IW715_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021285.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24290..24514)
                     /locus_tag="IW715_000663"
     CDS             complement(24290..24514)
                     /locus_tag="IW715_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021286.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24714..27482
                     /locus_tag="IW715_000664"
     CDS             24714..27482
                     /locus_tag="IW715_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1021287.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQSSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27563..27982
                     /locus_tag="IW715_000665"
     CDS             27563..27982
                     /locus_tag="IW715_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1021288.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27994..28464
                     /locus_tag="IW715_000666"
     CDS             27994..28464
                     /locus_tag="IW715_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1021289.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28481..29260
                     /locus_tag="IW715_000667"
     CDS             28481..29260
                     /locus_tag="IW715_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1021290.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29247..30068
                     /locus_tag="IW715_000668"
     CDS             29247..30068
                     /locus_tag="IW715_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1021291.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30085..31134
                     /locus_tag="IW715_000669"
     CDS             30085..31134
                     /locus_tag="IW715_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1021292.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31147..32151
                     /locus_tag="IW715_000670"
     CDS             31147..32151
                     /locus_tag="IW715_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1021293.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32784..33803
                     /locus_tag="IW715_000671"
     CDS             32784..33803
                     /locus_tag="IW715_000671"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1021294.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33846..34727
                     /gene="galU"
                     /locus_tag="IW715_000672"
     CDS             33846..34727
                     /gene="galU"
                     /locus_tag="IW715_000672"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1021295.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35027..35404
                     /locus_tag="IW715_000673"
     CDS             35027..35404
                     /locus_tag="IW715_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021296.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36010..36624
                     /locus_tag="IW715_000674"
     CDS             36010..36624
                     /locus_tag="IW715_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1021297.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        12174 a         6500 c         7591 g        10897 t
ORIGIN      
        1 accgtttatt cagataaggg actgtgacaa gacttttgtc acagtttctt tttgtttatt
       61 cttttttatc tttgtatggc aataatagac aatataacga aaattattta atggcttttg
      121 gaagaccatg gaaaaccttg tacaaagcta ttttttctga aaaattcctt gaaacctcac
      181 gaaaaaatca ttattttatg aaaaagacaa aaaaaagtgt tgatttttta ataattattc
      241 attaaactct taaacaactt cagaaaatat taattattct gaaaattcca ataaaggggt
      301 acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc
      361 tggatgttat ggcggcagtt cagccacaag cagtagttca gcaaaaagcg gttcagcaga
      421 tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg cctacagctg acttatcagt
      481 tgcaacagat acaatcagtt ttacagcatt gaataacgtt tatgaaggaa tttaccgtct
      541 tgatgaagac agcaaaccac agccggcagg agctagcgag ttggcagaag tcagtgaaga
      601 cggattaact tacaaaatca aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt
      661 tacagcagcg gattacgttt atggctggca acgaacagta gacccagcta cagcttctga
      721 atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac attacagctg gaaagaaaga
      781 taaatcagaa ctagggatca aagcagttgg tgactatgaa ttagaaatca aattgacgaa
      841 acaaacacca tatttccaat atctgcttgc tttcccatca tttttcccac aaaaacaatc
      901 cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt gataaagctg tttataatgg
      961 accattcaca ttagaagatt ttgatggacc aggaacagat acagaatgga cttataagaa
     1021 aaatgatcat tactgggaca aagatacagt aaaactttct gaaatcaaag tcagtgtagt
     1081 aaaagaatct tctactgcat tgaacttatt caaagatggc caagcagatg acgtgatctt
     1141 gtctggtgag ttagcacagc aaaacgcaaa cgatccagct tatacttctg ttaaagaagc
     1201 gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa
     1261 cctaagaaaa gctatttctt attctatcga tcgtgaagct ttagtaaaac aagtactagg
     1321 tgacggatca gttgtctcta caggattgat cccatcagat atggtaaaaa gtccaacaac
     1381 gaacgaagac tttgcaaaag aagctggaaa attagtttct tatgacaaag ataaagcaaa
     1441 agaatattgg gaaaaagcga aaaaagaatt gggcgttgat tctttagaat ttgatttgat
     1501 ggcttctgat gatgattcat cgaaaaaagt aatcgaatat gttcaaaact caatccaaga
     1561 aaacttggat ggcgtaacag tcaaaccaac acctgttcca ttctctgtac gtcttgaccg
     1621 ctcaacttcg ggtgattttg acgcagtact aggcggatgg gctgctgact atgcagaccc
     1681 aagcagcttt actgatttgt ttgtcacagg taattcatac aaccgtggtc gctggagcaa
     1741 tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc
     1801 ccgttgggat gatttccaaa aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc
     1861 ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca
     1921 tgcggcaggc gcttcatggg attacaaatg gacatacgtt gaagaataat cgttaatacc
     1981 gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc atattcactt
     2041 atcctaacaa agaaaaaagc atgaaaatag cagaaagagc aattagtatt ccggcaatga
     2101 tcagccaaaa gctgtacata ccgtagctgg cagacgagag cgaggaaaat tctggttttt
     2161 tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg
     2221 aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg caatgaccat aagaatagat
     2281 cattagataa ttgaatgagt gtaaaggaat ggttgatgag attttttatt aaaataacca
     2341 gaatcagtag aaaagctact gcgtagggga tagatttttt tttcatttga ttgctccttt
     2401 atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg
     2461 gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc
     2521 acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca
     2581 tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt
     2641 ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat
     2701 tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc
     2761 ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt
     2821 ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta
     2881 gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt
     2941 gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta
     3001 ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa
     3061 atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt
     3121 tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta
     3181 ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt
     3241 cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg
     3301 gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg
     3361 tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg
     3421 ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa
     3481 aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat
     3541 tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc
     3601 atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac
     3661 gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga
     3721 aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac
     3781 gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg
     3841 ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt
     3901 gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt
     3961 tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt
     4021 tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg
     4081 gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga
     4141 ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct
     4201 agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt
     4261 ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc
     4321 gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc agtaacttta
     4381 tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct
     4441 aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt
     4501 ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag
     4561 gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca
     4621 tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta
     4681 acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga
     4741 tgattttcca agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg
     4801 ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag
     4861 aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc
     4921 agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg
     4981 aaatcctgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt
     5041 tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg
     5101 accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg
     5161 ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat acttggggac
     5221 tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt
     5281 ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg
     5341 cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg
     5401 cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac
     5461 gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg
     5521 atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag
     5581 aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt
     5641 ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat
     5701 gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa
     5761 aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg
     5821 ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta
     5881 gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg
     5941 aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt
     6001 ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct
     6061 gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa
     6121 catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac
     6181 cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat
     6241 aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca
     6301 gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta
     6361 cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca
     6421 tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga
     6481 catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat
     6541 tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga
     6601 aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag
     6661 aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg
     6721 atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa
     6781 aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg
     6841 gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg
     6901 aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac
     6961 tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct
     7021 atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc
     7081 accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg
     7141 ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag
     7201 taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg
     7261 ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac
     7321 atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc
     7381 aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg
     7441 taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa
     7501 tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt
     7561 tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca
     7621 atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt
     7681 tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt
     7741 ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct
     7801 tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga
     7861 aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta
     7921 agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta
     7981 gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt
     8041 gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg
     8101 agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc
     8161 agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt
     8221 agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt
     8281 gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc
     8341 tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat
     8401 aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc
     8461 acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt
     8521 gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt
     8581 ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct
     8641 tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt
     8701 aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca
     8761 atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa
     8821 tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa
     8881 aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag
     8941 caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag
     9001 atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga
     9061 tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt
     9121 gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg
     9181 ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag
     9241 ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa
     9301 acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg
     9361 gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga
     9421 aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga
     9481 tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga
     9541 tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat
     9601 ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac
     9661 acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag
     9721 tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc
     9781 agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa
     9841 aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac
     9901 aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat
     9961 cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt
    10021 cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac
    10081 gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg
    10141 gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta
    10201 tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc
    10261 tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta
    10321 tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata
    10381 ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc
    10441 tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa
    10501 tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac
    10561 agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat
    10621 ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac
    10681 gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt
    10741 acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc
    10801 acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca
    10861 aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga
    10921 aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa
    10981 ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag
    11041 ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat
    11101 cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag
    11161 tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc
    11221 aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt
    11281 tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta
    11341 cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag
    11401 aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac
    11461 agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt
    11521 ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat ctatatttat
    11581 atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt
    11641 gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg
    11701 atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt
    11761 tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca
    11821 taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt
    11881 aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg
    11941 acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc
    12001 atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa
    12061 ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc
    12121 acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt
    12181 gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac
    12241 tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga
    12301 taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac
    12361 gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa
    12421 aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa
    12481 gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag
    12541 ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg
    12601 ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga
    12661 tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg
    12721 taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa
    12781 gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga
    12841 cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt
    12901 ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt
    12961 tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg accccaggac
    13021 aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat
    13081 tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa
    13141 tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga
    13201 gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg
    13261 atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa
    13321 tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt
    13381 atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca
    13441 gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc
    13501 agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt
    13561 tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa
    13621 aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta
    13681 ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag
    13741 cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa
    13801 aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct
    13861 agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact
    13921 tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt
    13981 gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag
    14041 cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt
    14101 gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat
    14161 ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact
    14221 ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg
    14281 aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta
    14341 ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa
    14401 gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa
    14461 cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca
    14521 ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat
    14581 gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt
    14641 tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa
    14701 acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca
    14761 agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc
    14821 agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca
    14881 tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat
    14941 aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca
    15001 agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt
    15061 agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac
    15121 tagacaagac cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga
    15181 aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt
    15241 gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca
    15301 acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac
    15361 atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa
    15421 ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag
    15481 gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga
    15541 tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta
    15601 ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg
    15661 aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga
    15721 tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag
    15781 tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg
    15841 ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc
    15901 agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag
    15961 tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa
    16021 cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag
    16081 caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac
    16141 aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt
    16201 gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    16261 ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16321 ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa
    16381 acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact
    16441 attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg
    16501 atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat
    16561 gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat
    16621 attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat
    16681 tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt
    16741 cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg
    16801 cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg
    16861 cattgcgagc attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc
    16921 atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt
    16981 tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt
    17041 actttattgg taatatgcct gaaacatatt attctcaagg atatttttat acagtattca
    17101 tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta
    17161 tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa
    17221 atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca
    17281 aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc
    17341 taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt
    17401 ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg
    17461 tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta
    17521 gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct
    17581 gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct
    17641 ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt
    17701 tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat
    17761 gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg
    17821 ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata
    17881 acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt
    17941 gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca
    18001 tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt
    18061 cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat
    18121 tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca
    18181 tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat
    18241 gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact
    18301 tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa
    18361 gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt
    18421 gatagattaa aatcagttac agaataattt tctgcattgt catatttttt atatacgttt
    18481 ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt
    18541 taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt
    18601 ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat
    18661 acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct
    18721 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
    18781 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
    18841 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
    18901 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
    18961 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
    19021 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
    19081 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
    19141 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
    19201 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
    19261 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
    19321 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
    19381 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
    19441 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
    19501 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
    19561 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
    19621 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
    19681 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
    19741 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
    19801 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
    19861 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
    19921 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
    19981 cgctaaataa aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg
    20041 tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg
    20101 tgcccatttc gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg
    20161 tggacaggcg atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt
    20221 gttggatttt tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca
    20281 tagggggatt actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata
    20341 tctttaaccc ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt
    20401 catacatata agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc
    20461 caaaacgaag attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc
    20521 caaaactttt acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa
    20581 ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg
    20641 cccctgtttt tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt
    20701 cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt
    20761 tgatattttt gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat
    20821 cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc
    20881 cagaaagttc gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca
    20941 caccaacagt gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg
    21001 ataaataagc aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt
    21061 gtgcccgttg tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag
    21121 cttcttcttg ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc
    21181 ccgagatatc cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa
    21241 cgatccaact cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg
    21301 gacttagacg catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt
    21361 gattgaaggc cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga
    21421 cgatatttcc gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa
    21481 atgcatcttt tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga
    21541 tgagccattg actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga
    21601 acagtcgatt gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt
    21661 gtaacgtcat cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa
    21721 tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt
    21781 tttttacttt cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga
    21841 ttgagcaagt aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa
    21901 gatatgatgt gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc
    21961 aagataagtc tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa
    22021 agagtcatct aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg
    22081 ctgtttttgt ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt
    22141 tggaagctga cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt
    22201 ttctgtggcg tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac
    22261 agaagtttgg ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac
    22321 atccattccg tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg
    22381 tgtcaaagtc gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt
    22441 cagctgaaat gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac
    22501 atggtcaaaa gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc
    22561 aagaatcgaa ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg
    22621 tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata
    22681 attgatcaaa gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc
    22741 aactgtcagc aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag
    22801 ggcagagata ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa
    22861 cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt
    22921 ctgactaacg tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac
    22981 ggtttttttg atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa
    23041 cagacctact tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg
    23101 tgctctgatc aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt
    23161 tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt
    23221 attgattttt ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga
    23281 agcatagtat tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca
    23341 taagcccatt tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga
    23401 agccatcatt aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc
    23461 caaaatgatt tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct
    23521 cctattattt atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa
    23581 tttagaatga aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt
    23641 agaaattgaa tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt
    23701 cattcatagg aacgtctatt ttacttaatt tttctataaa tgaattctgc ataaaaaata
    23761 tatcaagtaa atgtttatag atgaaactct atttacttct tttttaattt gttcttgcaa
    23821 tcttttttgc tacgtaattt ttgtaaaaaa gtgggttgag agaaatctaa ttgattttca
    23881 aagaaggagt atgaaaatga gaaaaatatt tattcgttta ttgtgggtac tatcttagag
    23941 gaataaataa tagtggagaa aactatggtt tggcgcagat tctatcaggt ttgatggaac
    24001 gaagacaacc tcccgacatc aagtcaaaag ctacttgaga ttttgatctg aaaataaaaa
    24061 aagaaaacaa ggtaggaaca tcaatgtttc taccttgttt tcgtaatcaa tgggaaaaaa
    24121 ctaagagtgg tgtacgtaat caataaaaag gatggtgaca acagtacctc tttactctct
    24181 gaaagcatca taactcgttt ttcctcatta tgccaataaa ttgttgttat accacatata
    24241 aggatttgta atagctaaaa acagacagga aagttgaaat ctgtgaatct tattttgtat
    24301 ggaaggattt atagaacgca aaaatcaaga taaagatgaa taaaaatcct gtttcggcta
    24361 tatattgcgt atctacgcca ataaaacgag tttgttctgt catagccata taaagcaaag
    24421 gcaaaaatac aactacagcg agataagatc cgatgatttc tcgaaaagtt ttccaggaag
    24481 tatgtttcct gtttttatat ttatccgatt tcattgaata gtcatagcct ccttattctt
    24541 tcatatgttg aaattccagt ataaaaactg gaaggaactg tacgtttttt agaataaaaa
    24601 ttataaggga taagtaggct gctgtctata ataagatata tttttcaatt ctttattaaa
    24661 gattttattg acaaaatgaa tccgttttct atgatggagg aggggtgaat aagttgttga
    24721 agaaggaaat tttatgttac ttagagaacc agacagcctt tttcgattta gaacatatga
    24781 acgaggtctt tacagcacaa aggttagctg atcattttgg ggtaaaaaga aacacagtca
    24841 gtcattattt gaaccagtta aatgatgagc aactactagt caaaatcaat tctcgaccag
    24901 tgatttattt ccacaaagaa gcttttgaaa aacaattttt taagttacgc acgaattttt
    24961 atttttctat cgatgatctc aaagaggaac agcctttttt cgcgcaacca aaagacttat
    25021 tctcttttat gatcggatac gacgccagtt tgaaagaaag tatcgaacaa atcaaaacag
    25081 ctctttacta tccagatggc ggattaccac ttttgattac aggcgaaagt ggcacaggta
    25141 aaagttattt agttaatttg gtctatcagt attgtctgct tcatgatctg ctagaagatt
    25201 ctgcaccatt catcactttt aattgtgcac aatatgcaga taatcctgaa cttttgacta
    25261 gcaatttgtt tggacatgtt aagggagcgt acactggtgc agaagaaaat aaaaaagggg
    25321 cttttgaagc agcagatggt gggatcttgt ttttagacga ggtacatcgc ttaacaccag
    25381 aaggtcagga aaaactgttc acctatcttg atcaaggtgt gatttatcgt atgggagaac
    25441 ctaatttgtc acgccggatc aaaacacgtc tcttttttgc tacgactgaa gagattacta
    25501 gtcatttctt aacaactttc attcgaagaa tacctatccg aatcgaacta cctgcactta
    25561 atcagcgtag tcgtaatgaa cggctagaac tgatttactc tttttttctc gaagaacaaa
    25621 gaaaaatggg gagccctttg tcagtcagtg gaccagcttt gtcactttta acaagtcaga
    25681 aattatccgg aaacatagga gagctgaaaa atatcgtcaa agtttcagca gcaaaagcat
    25741 atgctgaaca gcgtgaacag cctgaaatcc atgtcacgat ccatcatttg caaaaagaac
    25801 tacttgcgca gcctattact aaaaatacag cacagcaagg aatctatatt acgcaagacc
    25861 agaccttaga gcaattaatg gagaagcaac agcccgaaca gcagcgaatc gttaaaagtt
    25921 ttgagcaaat cctaattgat ttcaagaaaa ataactgtct gctatattct tgcgaaggaa
    25981 aattaaagca agaagtgatt caattatttg attttttgct gtttgagacc agtagacaag
    26041 aaaaacacga gctgctggtt tattggacac aatctatccg agagaccttt cgacaaatgg
    26101 aaactgccta tcaaatcaaa ttcgatggaa acagtgtcta tgccttgagt tactatctct
    26161 atcagcgaag cactatcaaa tggttaccgg aagaacatga tattatccaa ctaatcaaag
    26221 aattagaaca acaagtttct cagtcttatc catctagtta tcactatgtc caacgaattc
    26281 tggaattaag taagatcaaa ttagatattg aagacacaag tatggatcgg attttactga
    26341 cgatctatct aaaaaaagcg aaatggtcaa aagaaaaacg actgccaaaa gcgatgattg
    26401 ctgctcacgg ttatgcaact gctagcagta tggcaaatgt agtaaatcgt ttattacaag
    26461 aggatctatt tgaatcattt gatatgccac tagatgtcac accacagcag attgcttcag
    26521 aaatccttga ttattgcgaa aacagcgatg tttcaaatgg attgatcata ttagtagaca
    26581 tgggatctct gaaagaaatc catcagtttt ttaaaaaaca gtcatccgta ccgcttttga
    26641 ttttgaataa tgtgacgaca cctttagcaa tcacagtagg ggaatgcctg caaaaaaatg
    26701 tatcattaga agagatagca gaagagactg ttcgacagat ccagccagaa tggcgactgc
    26761 tctatccaga ggaaaacaaa cccaaagcac tcattactac ttgcttcact ggaatcggta
    26821 cggcggtcca cttaagtgaa ctgctagaaa aaagcttacc tacggcttgt cagttgaaaa
    26881 tcatcccata tgaatatcag caattaaaag ataagaaaaa cagtgaccca ttattttcca
    26941 tatacgaagt cgtaggaatg attggcacca cagatcctga tatcaccaat atcgactatc
    27001 tttcattaga agacctcatc tcgggggaaa agatgtctgt actagctgaa tggttggaca
    27061 ctactatgaa tgcttcagag aaggaaatat tcaatcagcg catcattcgt aatttttcat
    27121 tggaaaaagt actggattcc gtgactattc tagatacaga gaaggtgatg ggagaaatcg
    27181 atcattttat gagagaatta gaggtgcagt tgaatcgaag catcagcaat cccaaaaaat
    27241 tagcgttgta tgtccatgtg agctgtctca ttgaacgatt gatccgacaa atgccaatcg
    27301 aaacctatca aggattagag aaatggaagc agtgtcaaaa agaagggtta tctgctatta
    27361 aatctgcgtt tagtgtcatt gaagagcatt atagtgtcat gatccccaat tctgagattg
    27421 cctatatcta tgatattttg agtgaagata cagagtttct ctcgatagaa gaagaatttt
    27481 gaacataagc aagttgttgc cactaatttt ggcatgcaac ttgctttttt agtatgtgta
    27541 agtgaaaaga ggagatgaaa aagtggatag agaaattatc ttagcttccc atggaaaatt
    27601 tgcttcaggg atattagatt cattagagct gatctatggg aaaaatcatt ctattactgt
    27661 attcgattgc tatactgatg agaattttga tttagcggaa acagtaaggc agctttttat
    27721 gaggtacaaa gataaagaaa tcattgttct gacggatctt tttggcggaa gtgtcaacaa
    27781 cgaattctta caatatatca atcgggatca tgtctatctc gttgccggat tgaatttgcc
    27841 actattgatt gagctagtat ctcggttgga cgctgaagta gagacaactg ttttgatcca
    27901 gcaggtgcta actgattcaa aaaaaatgat ccagttttgt aacgagcggc tcaatcagaa
    27961 aatagaagaa gacgaatttt aggaggaaga aaaatgattg tattgacaag agtcgaccat
    28021 cggttattac acggacaagt agctttttca tggacacaaa caataggcgc agactgtatt
    28081 ttgattgcta atgatgatgt acctacgaat gagatacgaa agacaacgat caaattagcc
    28141 aagccacaag gagtcaaact agtgatcaaa tcaattgatg actcgattgc cgcgttgaaa
    28201 agcggggtga cagataaata taagttattc atcgtagtgg aatcgatcga ggatgcatat
    28261 aagctggcag aagcgtatcc agaaatcaaa accatcaact taggagggat caaggcaaaa
    28321 cctggtactc gcagcatcgg aaaagcggta aatattttac cagaagagga ggaacgcttg
    28381 aaacgattaa ttgacaaaca aatagaagta gaaatccgtc aagtaccagc agataaaaaa
    28441 gtggatgtaa gagaagtttt gtaagaaagg aagaacctca atgattgtac aagcagtctt
    28501 attaggaatt gttgcatttg tcgcacaatc agaatatgca ctagggacat cgttgctttc
    28561 tcgtccgatt gtgacggggc tgtttacagg aatcgtgtta ggtgatgtaa aaactgggat
    28621 cattatgggc gcaactttag agttagcatt tatcggatcg ttctcagtag gtgcttcgat
    28681 tccacctgat gttgtgaccg gtgggatttt agggacggct tttgctatta cagctggagc
    28741 cggtacagaa accgcgcttt tactaggttt gccgattgct acgttgacat tgatattgaa
    28801 aaatatttac ttagggcttt tgattccaat tatgaaccat aaagcagaca attatgcaga
    28861 agaaggaaat tataaaggag tcgagcatat gcacttgctt gccggtatcg gtctttcctt
    28921 gatgcttggt gttgtggtaa tggtttctta tatggtggga agcaatacga tcggaaatct
    28981 attaaatatg attcctgact tcgttcaaaa ggggttgtca gtagctacag ggttgattcc
    29041 agctcttggt tttgcgatgc ttgctcgttt gctgatcaac aaacaagttg caccatattt
    29101 tttcttaggt tttgctatag cttcttactt agagattcct gtgaccggag tagctatttt
    29161 tggtgcgatt ttagcagttg tggtagtcaa tattatgaat gtccgtcaac taccacttca
    29221 aacaactgca ggggaggtag aagaagatga agaattctga gatcaaaaac agcaaaataa
    29281 cgaaaaaaga gctaaatcac gtattttggc gttcttttca aatggagttt tcttggaatt
    29341 atgaacgtca gatgaatatg gcttatgttt atgcaatgat tccgattttg aaaaagttat
    29401 atcagtcgaa agaagaaatg gcagcagctt tgaaacggca tctagagttt tttaacacga
    29461 ccccacatat cgttacactg attttaggaa tcacggctgc aatggaggaa gaaaataagg
    29521 atgatcctga atttgatgtg actgcgatcg atagtatcaa aacatctttg atgggacctc
    29581 ttgcagggtt aggagattct ttcttttggg gaactttgcg attgattgcc acaggtgtgg
    29641 gtacctcttt agccatgcaa ggaaatattt taggccctat cttgttcatt ttgattttca
    29701 atattccgca cattctgttt cgctatctag caacaggttg gggatatcgc ttaggaacgg
    29761 gttttttgaa aaagatccag gcaaacggta tgatggaaag tttgacatta ggcgcgtcta
    29821 ttattggact gatggtcgtt ggagcaatga ctgccacgat gatcaccatc aatattcctt
    29881 taaaaatcgg ttcaggagaa aatgctgtga ctgttcaaag tatttttgat gatatcgttc
    29941 caaatatctt atgtcttgga gcatttggca tagtgtttta tctattgaag aaagaagtga
    30001 aaccattaac gatcttgatc ggactagctt tctttggaat ttttggttcg tggataggga
    30061 ttttttaaaa aaggagcaga gaaaatgttt acgatgcaag attatatcta tgaagaaaag
    30121 gaagtactaa gtacgatctt gaaaaagaat gatttttcga caagagaaca tatgaaaaaa
    30181 acagtgaatt tactgatctt agcaacgggt tcatcttata acgcttgttt agcagcgaag
    30241 ccagcattgg aatcttatgg tgatcttact gtcgatattc aagaaccttt ttattttttc
    30301 aattatggaa aactttctcc ttcaatcgat actgtgattg ctgtgtcaca aagcgggaaa
    30361 agtgcttcta ctgttgaagc agtaaaaatg atacaaaaac aagggttacc tgttgttgct
    30421 atcacaaatg acgtgcaaag tccacttgct ttagaagctg cacaaattat tgatttaggt
    30481 gccggggtag aatcagtagg cttcgttaca aaaggctatt ctgcgactgt tttacagtta
    30541 ctactcttag ggataggaat tggaataagt aaaaataaaa tcagcaaaaa aatagaacaa
    30601 gactacatgc aacagttgcg caaaataatc aaccatttac cagcgattat tcaaaaaacg
    30661 gaagaatttt ttgatggata tcaaagttta tttcgtttag cacagcgttt cgtcaccatt
    30721 ggatatggtc caaactgggg aacagctaaa gaagcagaga cgaaattgac tgaaacaatc
    30781 cgcgttccgt cacaaggatt tgagttagaa gcttatatgc acggcccata cctggaggca
    30841 gatgcttcgc atttgctatt ctttattgaa ggggaaagtg taaataagga acgttcgcaa
    30901 aagttgcaaa gatatatgag tcggtacgtt ggagagactc tcaccattac cacaaaaaaa
    30961 gcaagaaacg agaaaacact tggattagca atagaatgtg atgaatatct gtctgtatta
    31021 gcgcttgtcg ttccgtttca actattcgct tataaaaccg cagtagctaa aggtattgac
    31081 ttgaacaaaa agatttttga agactttgat acagtcttga aaagtaaatt ataagaggtg
    31141 gaaatcatgc tgaaattcaa tgaagaagaa caaatcaaag caataaaagg cgcactagct
    31201 ttacgtccgc aagtagaaga aatcatcgat aaactttatg cggaaaaatt tgacgctgtc
    31261 tattatctag gaatcggtgg aacgtatgct tcctcgatgc aagctgtcac ttatatgaat
    31321 gggaaaagca acttgcctgt ctttgtacag catgctgcag aatattacac tactggaaac
    31381 aaaagattaa caaaagattc tatcgttatt ttgtcctctg ttaccggtac aactcaagaa
    31441 gtagtgaaag cagtagaaca aatcaagaaa gtaggagcga cactgattgg atttatcgat
    31501 aaagcgaaca gtaagttgtc tcagctatgc gattttgtgg tcacttatcc agctcctgga
    31561 acagaacaaa tcaaattttt tatggcagca gatcgattga tgtatttgaa tggagaattc
    31621 gaagcttatt cagaatacta tgaacagctc gaacgttatt taccaacagg gttagtggaa
    31681 gcagagaaaa aagcagatgc atttggctta tcatttgcag aaaaacatcg acgtgatagt
    31741 atgcattatt ttattggagc agggaatcaa tggggagctg tttattcata tgccatgtgt
    31801 tactgggaag aacaaagctg gcttccatcg aaatctatcc atgctgccga gtttcttcac
    31861 gggacgttag agatcgtaga agaaacaacc ccagtcacat tatttttagg cgaagatgaa
    31921 cagagagtgt tatctgaacg tgtggctaaa cttttgccaa aaatttgcag caattataca
    31981 ttgatagaca caaaagacta tccagtagaa ggtatcagtg aaaaatatcg cggacgtgtt
    32041 ttgtctttct tattgatgca tgtagtgaca caacgcatcg atgctcatgt ggagcagttg
    32101 aactgccatc cattagagat acgccgctac tatcgtcagt ttgattatta ggatagactg
    32161 gataaaaaga atataaaaaa taaagagagc agcgataact gctcccttta tttttttaaa
    32221 atattggcat caattacgtt ataaaacaca ttttttgcat tatctacagt tcaacttata
    32281 atctgtttaa tgggacatgt gggtgccaac cattttttgt tatgcaatag tactatttag
    32341 ttgttgcatg gatccgtagt aaaagtccaa gtacaacgag ttgcgccagt ttacatagcg
    32401 ttttgtatcc agaatgttga ctaaaacaaa agttcctatg tcaaccgtca acagagacaa
    32461 tctgtccgtt gcaggacctg acagatttag aaatgttcaa tctgtctctt atgtatttta
    32521 aatcaatact cactaggaga agtttcctaa cctcctaata gtaatgctgg tagtaacatt
    32581 gtcaaaactg cttcctctta ggaacaagca aagcataacg agtgtaaaaa taaaatgtag
    32641 actattgtaa catatcattg attatttcaa gtaaaaacga taaaagaagt aaacaaacac
    32701 ttaaaaacaa tggaaattag tcccaacttt aagaaatgta tactataatg gaaattatca
    32761 attatttatt tggaggaata tccatgaaac aaaaaatcgc agttcttggt cctggatctt
    32821 ggggcactgc tttagcacaa acattggcag aaaacgggca tgatgtccgt atttggggaa
    32881 atgtacctga acagattgat gaaatcaata agtatcatac aaatcagcat tttttacctg
    32941 atttgacgat cccagaatcg attatcggat ataaagaatt ggcagaagca gtggataacg
    33001 cagatgctat tttatttgtt gttccaacaa aagcgattcg ttcagtcgca caggaactta
    33061 ttggtaaaat gaatactaag ccaatcatta tacatgcaag taaaggacta gaacaagata
    33121 cgcataaacg aatctcagaa gttttggctg aagaaattcc agaagaaaaa agacaggcaa
    33181 ttgttgtatt atctggtcct agccatgctg aggaagtggc agtacatgat atcacaacaa
    33241 ttacagctgc aagtattgat caaaaagcag cagcctatgt acaaaaactg ttcatgaatg
    33301 attatttcag gatttataca aataatgatg tgattggcgt agaaactggt gcagcactga
    33361 aaaatatcat tgcaattggt gccggcgcta tccatggatt aggattcgga gatgatgcaa
    33421 aagcagcaat catgactcga ggattggcag aaatcagccg tcttggtgtc gcgatgggtg
    33481 ccaatcctct gacatttatc ggtttgagtg gtgtggggga cttgatcgtt acttgtacaa
    33541 gtgttcattc tcgcaattgg cgtgccggta acttgttagg acagggacat aagctggaag
    33601 aagttctaga aaacatggga atggtcgttg agggtgtggc aacgacaaaa gcagcagtag
    33661 agctagctca acagctaggt gttgagatgc caattactca aacaatctat aatgttttgt
    33721 ataatggaga agatatcaaa aaagcagcaa aagaaatcat gttacgtgac ggaaaaatgg
    33781 agaatgaatt ttctcttcgt taaatagatg atcaatagtt aatcgtcgtt taggaggaac
    33841 agcaagtgaa ggtcaaaaaa gcagtcattc ctgccgctgg gctaggaaca cgttttttgc
    33901 cggcaacaaa agccatggca aaagaaatgc ttccaatcgt ggataaacca acgatccagt
    33961 ttattgtgga agaagcattg gattcgggaa tcgaagacat cttgatcgta acgggaaaag
    34021 aaaaaagacc cattgaagat cactttgatg caaatatcga attggaaaac aatttgcggg
    34081 aaaaggaaaa aacagaatta ctcgctttag ttgaagaaac gactcaagtc aatcttcact
    34141 tcatccgtca atctcatcca agaggactgg gggatgccgt gcttcaagca aaagcattta
    34201 taggaaatga accgtttgtc gtcatgctgg gggatgattt gatgaaagat gatatcccat
    34261 tgactcaaca attgatgatg gattatgaag aaacacaagc ttccgctgtt gccgtcatgc
    34321 gtgtaccaga aaatgaaaca tctaaatacg gaattattga tcctaaaaca gatagcgaaa
    34381 agggtcgttg cagagtaaaa gggtttgtag aaaaacctga aatgggaaaa gcaccaagta
    34441 atttagccat tatcggacgt tatctactga ctcctaaaat atttgagata ttagaaactc
    34501 aagaaccagg agcaggaaac gagatacagc taacggatgc acttcagact cttaaccaga
    34561 cagaggcggt ctatgcgaga gagtttaaag gaaaaagata cgacgtaggc gataagttag
    34621 gatatatgaa gacaaatatt gaatatggac ttcaacattc ggaaattggg acttcattaa
    34681 gtgcatatat tatccagttg agtaaaaagc taagaacaga aagttaactg gaaataagtg
    34741 gcctgctgag aatgaaaggc tatttgttat taaagcggca gcttgtgcgt catttgcgca
    34801 actgtcgttt tagttttttg actctagatc agttgagtcg tggaaaacga aagatagaaa
    34861 aagactttcg tgaatagatt cagaaaatat tatgctaatg gtttaatttt tttttaagtt
    34921 gtataaaaga aaatgattac ataagagaaa tgaaagccaa gccagaagat tcatagtaga
    34981 ataaaaaggc gaatttttta attataaaaa aaggtgtgtg ttcactatgg gaaaattagt
    35041 cgctgttgca cagtcgctgc ttgatatact caagtggctg atggaaggaa gcaattggat
    35101 atttgtgttg ccactattgc ttattagtct atttatcatg tttcgcatgg tcagaaaaaa
    35161 agaaacgttt ctgattttta tctggtatgc agcatccgtt ggggttgcta ccttgatggt
    35221 tgaattggcg ttaggtagtt gggaagcttg cgtatttgct ttattttttg ctgtactgta
    35281 tctaatcatt tatatcccat atatgaaaag aaagtctcgg ataggcagaa aaggtagaca
    35341 tacaactact ttttctagta cagattctct cgaacaaagt agaagaacga gaagaagacg
    35401 gtaatattta gtaggtatac gcaaaaaaag ataaaataat ggataaacta ttgagatgag
    35461 agatttattt aattggtgga aaaaagcttt tttcacttat ataaaagaat cttttttaaa
    35521 ggaatgcttg ttgtatcggg ataagccatt ccttttttta tgttatttat atatatataa
    35581 atcataccaa tgtttttttg tattttcttg cttgcacttg gataagagat tacttacaat
    35641 aaattcagat agaaacagct gaataaaaaa aggctccctg acccttagtt ggcgctaaga
    35701 atcagggaat gaccagtcac acacatacac cctgaccgat cattttgcca tagtcaatgc
    35761 agtgcatcga cctaatttat tgtacctaat ctagggtgaa aattctaccc ctatgtagat
    35821 attttccatg gtctgcacgt tgttttattt gctatgtcaa ggtattgggc gtattcgttc
    35881 aatgcctttt ttgtgtttga aacagcaaag accacatgtg ttactgtcta actactgttt
    35941 ttttatgtac ttccttagat taggcagctg tttctattca tgtcaagtat agctaggcgg
    36001 gagagataaa tgagtttatg ttattttgac tattatcacc aattggaaga gaaattaagg
    36061 atagaagagc cgctgatgct tcaagctggc acttggatcg cgaaacaaat taatcaaggt
    36121 ggtagattgc agatatatgc tagccgcaca ctaagtggtg tagcatttga attttgggat
    36181 caaaccccgg atcttgtacc aagtatattg attgaaaatc cagccggagg tgtctatgaa
    36241 acgctagaag gtgcaggaca ggcaattatt gatcagcttt ctgtcaaatc agaagacatt
    36301 tttcttcttc tttcgaatga aggaagaaac ccggcaatca tcgaactagc tcagtggatc
    36361 aaagaaaatg gtcatccatt aatcattgtt actggatttg atttgtcacg aagtatcaaa
    36421 tcgcaacata gcagtggttt gcgattattt gaatttgctg atttggtttt ggacaaccac
    36481 gccaatttac atgatgcagt cttgactttg ccaaatctag atccagcgat ctgcggaaca
    36541 gcctctcttg caacgatctt tttgctgcag caagttttgt attttacagc aaaacagcta
    36601 gtccaggaca caagtggaaa atagttatcc acatgttgac aaagttcctt aaaaaaataa
    36661 tgttccacat aaaaatggag cgtttgaaaa aaagaaaacg attattaatg aggattaaca
    36721 acgactactt tttttgtttt agttatttta agatggatca tctttgattt taagcttgtt
    36781 ttgtttaaag tttgttattt ttataagttg tttcacagtt atgagaaatg agttataaga
    36841 atatgtagtg tcagaaaaat aaaaagtagt ggaaaaagag acaagaaaga atccatatgg
    36901 attctttctt gtctcttaat acacgttgat gaaagagtct tccccaaaac tctttacaaa
    36961 gtaattatag tacatttgtt atcattattg caatgaagtt tacctgatat aacaaaattg
    37021 tccaacagga tacctttttc taatagaaat aaaatacaca aaaattgaag gatagataat
    37081 ggatatttat aagtgtttat tatttttttt gcaatgaaat tcaatttttc aatgattgta
    37141 aaatgaaaaa aacaggacga ga
//
LOCUS       DACEMK010000016        36940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEMK010000016 DACEMK010000000
VERSION     DACEMK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.016"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(96..524)
                     /locus_tag="IW715_000675"
     CDS             complement(96..524)
                     /locus_tag="IW715_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021298.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(552..1190)
                     /locus_tag="IW715_000676"
     CDS             complement(552..1190)
                     /locus_tag="IW715_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021299.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1194..3230)
                     /locus_tag="IW715_000677"
     CDS             complement(1194..3230)
                     /locus_tag="IW715_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1021300.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3356..4021
                     /locus_tag="IW715_000678"
     CDS             3356..4021
                     /locus_tag="IW715_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021301.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5956..9105)
                     /locus_tag="IW715_000679"
     CDS             complement(5956..9105)
                     /locus_tag="IW715_000679"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ1021302.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9118..10317)
                     /locus_tag="IW715_000680"
     CDS             complement(9118..10317)
                     /locus_tag="IW715_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1021303.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10307..11902)
                     /locus_tag="IW715_000681"
     CDS             complement(10307..11902)
                     /locus_tag="IW715_000681"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1021304.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12486..13193)
                     /locus_tag="IW715_000682"
     CDS             complement(12486..13193)
                     /locus_tag="IW715_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021305.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13258..14094)
                     /locus_tag="IW715_000683"
     CDS             complement(13258..14094)
                     /locus_tag="IW715_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1021306.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14112..15521)
                     /locus_tag="IW715_000684"
     CDS             complement(14112..15521)
                     /locus_tag="IW715_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1021307.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15535..17358)
                     /locus_tag="IW715_000685"
     CDS             complement(15535..17358)
                     /locus_tag="IW715_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1021308.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18201..18527)
                     /locus_tag="IW715_000686"
     CDS             complement(18201..18527)
                     /locus_tag="IW715_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021309.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18573..18935)
                     /locus_tag="IW715_000687"
     CDS             complement(18573..18935)
                     /locus_tag="IW715_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1021310.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18972..19187)
                     /locus_tag="IW715_000688"
     CDS             complement(18972..19187)
                     /locus_tag="IW715_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1021311.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19314..19649
                     /locus_tag="IW715_000689"
     CDS             19314..19649
                     /locus_tag="IW715_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1021312.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALPRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19725..21722)
                     /locus_tag="IW715_000690"
     CDS             complement(19725..21722)
                     /locus_tag="IW715_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021313.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21820..22095)
                     /locus_tag="IW715_000691"
     CDS             complement(21820..22095)
                     /locus_tag="IW715_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021314.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22327..23061
                     /locus_tag="IW715_000692"
     CDS             22327..23061
                     /locus_tag="IW715_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1021315.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23100..24242
                     /locus_tag="IW715_000693"
     CDS             23100..24242
                     /locus_tag="IW715_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1021316.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKVSTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24337..25728)
                     /gene="sufB"
                     /locus_tag="IW715_000694"
     CDS             complement(24337..25728)
                     /gene="sufB"
                     /locus_tag="IW715_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1021317.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25733..26203)
                     /locus_tag="IW715_000695"
     CDS             complement(25733..26203)
                     /locus_tag="IW715_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1021318.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26190..27425)
                     /locus_tag="IW715_000696"
     CDS             complement(26190..27425)
                     /locus_tag="IW715_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1021319.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27422..28708)
                     /gene="sufD"
                     /locus_tag="IW715_000697"
     CDS             complement(27422..28708)
                     /gene="sufD"
                     /locus_tag="IW715_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1021320.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28721..29494)
                     /gene="sufC"
                     /locus_tag="IW715_000698"
     CDS             complement(28721..29494)
                     /gene="sufC"
                     /locus_tag="IW715_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1021321.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29691..30539)
                     /locus_tag="IW715_000699"
     CDS             complement(29691..30539)
                     /locus_tag="IW715_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021322.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30563..31249)
                     /locus_tag="IW715_000700"
     CDS             complement(30563..31249)
                     /locus_tag="IW715_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021323.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31242..32276)
                     /locus_tag="IW715_000701"
     CDS             complement(31242..32276)
                     /locus_tag="IW715_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1021324.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(32671..33009)
                     /locus_tag="IW715_000702"
     CDS             complement(32671..33009)
                     /locus_tag="IW715_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1021325.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33011..33367)
                     /locus_tag="IW715_000703"
     CDS             complement(33011..33367)
                     /locus_tag="IW715_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1021326.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33373..34560)
                     /locus_tag="IW715_000704"
     CDS             complement(33373..34560)
                     /locus_tag="IW715_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1021327.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34751..35596)
                     /locus_tag="IW715_000705"
     CDS             complement(34751..35596)
                     /locus_tag="IW715_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1021328.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35830..36795
                     /locus_tag="IW715_000706"
     CDS             35830..36795
                     /locus_tag="IW715_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1021329.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10955 a         7582 c         6122 g        12281 t
ORIGIN      
        1 atacaactaa aaatgaatat attaaaacta ccaaaaataa cttttaacgc tgtttcatta
       61 tatgtttttt ctattttata taaacttttg gatacttaat accaaccggt tggattgtac
      121 caacatgaga atgtgtcagt tctatttcct gtcgcacttg cataggccca tactcctttt
      181 gttgcatttg ctgatcgaat agagtttcca actttcgctt ttgcaccgtg acgattatag
      241 acacgactat agtggttaga atgacctgtt tttctcgtac aagttgtatc actataataa
      301 acccatcttc cagagctatg attaattgct gttctagaaa tttcccggtc tactggtaaa
      361 tttgacttaa taatattacc tttagcatca aaatgaatct ctcctggctt taaagatttg
      421 taatagattt caccgtgaac tgaatttgct tccacttcag atgacatagc gtttcctcca
      481 acaaaaccta tccctagtgc acaaataata actgcttttt tcataaaacg acatcctttc
      541 ctttttgatt tttaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc
      601 gtgactacga taactaacgt accttgggaa gcttgttgcg ataagatttc aactacttta
      661 tccttatttc ttttatccaa tgacccagtc ggttcatcgg ctaaaatgat tttgctattc
      721 ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata
      781 ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc
      841 ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt
      901 tcatattcaa tcaacccaaa atcttgaaaa agaaaagata tggtatttct ttttatcaag
      961 cgcttctctc tcccagttaa ttgtttcgtc tcttgatcga acaaatagaa ttgtcctgaa
     1021 ctgaactgat cattcaatcc taatatattc aataacgtac ttttacctgc tccactttct
     1081 ccaacaacag tcacaagatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt
     1141 ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct
     1201 ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga
     1261 ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata
     1321 agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct
     1381 ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg
     1441 tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt
     1501 aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca
     1561 gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc
     1621 tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct
     1681 ccgctgagaa tatttctttc caaatacata ctgtttttca atgtcagtat ttcaacaacc
     1741 ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt
     1801 ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc
     1861 aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt
     1921 tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga
     1981 taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct
     2041 gatacattga ctaatatact tccgttttta ttcaaagttt catataattc ctgatctgct
     2101 gctgatgcaa aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga
     2161 taaaatacat gatagtcctc accagcgatt tcttttggtg gttcatggga aacagctaac
     2221 tgtgaagaag aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa
     2281 aacaacaatc caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa
     2341 gcgatttttt caactaataa aagagaaatc aaagagatac catatattcc gctgaacaag
     2401 aaaacgtaca tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca
     2461 ccaatgattc ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac
     2521 aatacttgat gtaatgaaaa gtcattattc aacaataaac tgatttcttt attcatcaat
     2581 aagaaaaagg atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga
     2641 taattatata cgtctgtatt tgaaataacc gaatacgaat agctattttc aaagtcttca
     2701 aaaatattag tggcataatc agtgtgaaat gcttgattga atcgatcagt gaatagtgaa
     2761 atgacccgtt cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct
     2821 tttattttga tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc
     2881 ttctcgtgag aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt
     2941 atcttgaagt tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa
     3001 gaaagctcag tttctgatga gacattttca aataccatga ttagctgctc atattcttct
     3061 tgcccaataa aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat
     3121 tcatttgtcc gatagagtac ctgtttattt atactaacca aggcaaaacc actcatgata
     3181 ccggtcacta tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct
     3241 tacttaaatg attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa
     3301 gtgtataggt catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa
     3361 caccggagaa aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg
     3421 cgaaaaaatt ggtgtttccg ataaaacgat ttctagttgg gaaaattctc gtacaatgcc
     3481 agactttgaa atgctgtcac ttttacatca tactttaggt ctgccgattg attttccttc
     3541 tattgaatca gctaccgaat caatagaacc aacattgcca gatgaacctc ttgttaacaa
     3601 gaaatcttat tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct
     3661 cgtcttttgt ttggtctatc tttttcttat caattatcaa tacaaaaatg aaacgatcga
     3721 tcgattcaat ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt
     3781 ggacaattcc atcgaacgtt gggtatgtaa tgatatcttt ggccatggta catggctgtc
     3841 ttttcacgct ggagaggtag aaaaagagaa aaaccatgct gtaatcaaac acaaaggaaa
     3901 acacgtcatg aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat
     3961 cctcagccaa gactatgacg acgccttaaa aagaagccct caggagccag ataggcatta
     4021 agtaggagat gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc caaaaaacag
     4081 gggtacaaat taattgaaaa agccttgaga tgaaagttcc taaataaaaa agttcagaat
     4141 gataggtatt tctggcttat agtataatat ttacgccaca gcctcccaat gattcttttt
     4201 ggaatcataa tcaaggatgg acattcacaa aaatattttg ctcattcgat ccgaatagat
     4261 taacgaccaa tctagtaagt aatatagatt ggtacgtagt caagctgcac ataatttaga
     4321 tcaattttag ttctttttta tatttctatg atctgctcat ttacttttat atgatggctt
     4381 acagtaacat aaataaatat gataatcgct ggtagtttat ccaagagtac tcaataaaat
     4441 acaaaatgga ttgttcaagc gcctatcata atgataaaat gaaacaaata aacgatacaa
     4501 ggaggaagag actttgagta atggcagaac aattttttca ttgtaaaaat tgttcagttg
     4561 ttaatctatg ggatttcggt agcatgggca tttttttata gaggaatcac accaacctgg
     4621 ccgttttatc tcttgttagt gggaatatta gtaggaaatt ttgcccctct tcactatcgt
     4681 acgaactttt ttccaactga ttctggcctt tttcttaaaa caaagacgcg aaaattagaa
     4741 aacattattg aaatgacaat tacctgtata atcattttct tagttatatt cctaaacgtc
     4801 ttctaataag aatctcttaa aattagcttg ttacagttag ttacctagtt tatataacac
     4861 agttatctta tcttccttat caacatcttt tgccaactct ttgtcttaaa ttcgaaacat
     4921 ataagcagag ctacgaacac caagactgtt tcggctctgt tttttgcatc aaattcactc
     4981 ttatatagct gataatctcg gctctttaaa agaaattatt ttcgcttcat tttattataa
     5041 aaaggctagg aaccttgatg ttatcaggtt cctagccttt atttaagtat taccccacag
     5101 acccttccat atagattttt aaattacaca ctagtttaaa ctaaaacaaa caaggaaaaa
     5161 cttaccattt aatcaaaaaa ttagctcata tgaatatatt agaatgtaaa ttagactggt
     5221 caaaatctgg tcaaaaaaga ggacgaatac aggtagtcct cttttttgtt attttgatat
     5281 tctaaatcat tttttattgg attattccct tgttcgatat agacccaaat tgatctatca
     5341 ataattatca aactgagtca tatagagaca aaaatttcaa aaatgacctg ttaaatggtc
     5401 atcgaatatc atttaaaatc aatacgacgg agtcagacac ggagtcaaaa caagcccgct
     5461 gagagtaaaa aatatacagt cccataggta aagtgccaca ggactgtata tttttttatt
     5521 tgagattacc tgaaatgatt ccatttaatt tttatctcat tttttcctac tagttagtat
     5581 caaatactct cctattttct caataaacta ttttgggtta catggtatct gaaacagcta
     5641 aaaccttgtc attatagcaa aaaatcactt tatctccgtt tcatttcaga tacatttcac
     5701 ttaagcggat tccaaaaaaa caaaagaaaa aggaacccct agttagccta tgctttttat
     5761 tggttcctct caaaacacgg tcatataaca acggggtggg gtaaatgaat gaaaaaggtg
     5821 gaggaaacat tattcaatag ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa
     5881 gacatctatc cctattttct acacttccac ttgactacca tctttgaatc gtacaatttt
     5941 cttaactctt ctcgtttacc tcaattcatt ctcaagtgga atcaactcat catctaacat
     6001 tttacgccat gctcgcttca tttcttgtgg gtatttaaat ggcttcgctt ctggatgttt
     6061 ggctttatat ccttcgtaat ccttactgtt gataattgct ttcatattag gaattggatc
     6121 ttcacctggt agatattgca gattagaagc atgtaattct ttctcagaaa caaaccactc
     6181 attggaaaag acatgaatca aatcatcact cgcttgtgta aaatagcgac gtttaatgac
     6241 aaagatatct tcattactat ctaaagtccc cacatcaaat gcatttaaaa tttgtcggta
     6301 aacttctttg actccttctg ttttattcaa tttagccaaa gctttttcaa tttcttctcg
     6361 tgcatctgaa ctattcgcag cataagttcc tagtaattga tcaatataag ttgaatcaat
     6421 atcatacaac ttagtggtat tttcactata aaattcgatc gttgaaaaat cttctccttc
     6481 tttttctggt tcttcctcta ttaatgaacc cttcacggtc tcgtacacgc caatttgttc
     6541 ttccaaaagt tgaatttgat tgttcaaagt tggagatttc accatgtcat cattgtaatc
     6601 gtcataagtc actaacgatt cataagcacg attcaattct tgaaatgctt tgacgtactc
     6661 gatacggatt tcaagtggcg tatgttcatc aatggattcc atggatgaaa cctcttttaa
     6721 cttaccatat gcttgtttga atcttttttt ggattcctga taacttggat aaatcaaacc
     6781 tgattcttct tttgcttttg aatacagttt tgtcgcatcc tcgacattct tctccattgt
     6841 cgctggctta cgaaacgtca caatcaatcc ttttgtctta tcaggatacg tacgattcgt
     6901 acgtgaaaaa gcttggatca atcctgcata ctctagatta cgatccacaa atagggtttg
     6961 aatcgtaggg gcgtcaaaac ctgttaatag acgatccact acaatcgcta aatcgatttg
     7021 tttcccaaat tgtttgaatt ccgctctttt tcgtgccaaa cgattattga tgtctccgtt
     7081 ataacgctca atgtctgcta atgaccagga agtatcataa tactgattat attcctcaat
     7141 aattttttgc atttcttctt gttgggcact tgaatctcgg ctattttctt ctaatgaata
     7201 ggtaatagcg attcgtggaa aatcaggatc ctccatcgtt cgtccttcac gaatgagctg
     7261 attgggaaac tcttttgtta accaatcagg ctctttcgtc atctctttga tggcccgata
     7321 atactttttg gccatgtcaa tggaactcgt ggttaagatt gcagatttcg ttggcctgcc
     7381 atttcgaaaa tcaaattttg tataggcatt atctggacgg aacattttat gaatgacttt
     7441 ttggatatgt tcatctgctt caaaaacagc tgggtccaaa taactttctt ttttgacagg
     7501 ttccatttga tcaatcatac gattgatctg ttctgatgaa tactctgcat atgtttctac
     7561 ttcttttaat ttccgataga ttttattctc tagtgaagtc ggctccacgg tgttttcatg
     7621 ctctacttga aatcctaaga cagagccatc ttctagcgca ttcttaatgg tataggtatg
     7681 caagacctct ccatactgat catgggttgt acgtgccaat tgccctttgg cttgtttccg
     7741 attctcagga aaaatcggtg tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt
     7801 tttgatttct tccatatttt cagcacttaa cgctcgatga cactcatcta ctacaaacac
     7861 gatatgttgg cccattaatt tttggaagcg attggttcct tttttctctt cttgtttctt
     7921 tgcatatttc aacgccgcat ctagtttttg acgggttgta atgattacaa catttgcatt
     7981 ggcatctgcc aacagggttt cacttaattc tttggcactt cccgttccaa cgatcaagct
     8041 attcgctttg gcgttcccgg aagaaatccc agtattaaat tccgaagcaa attttgtaaa
     8101 ctctgtggtg gtttgattat ctaaatcttt gcggtccact aacatgatcg ttcgatcgat
     8161 ccccgatttt cgtgctaaaa gcttagtaga tacaaaacta gtcaacgtct ttcctgaacc
     8221 agtcgcatgc cacacatacc ctgattgatg tttatttgcg gcaataaaca aggcttgaat
     8281 cgcatgtact tgataaggat gtaacaccat gagggtttta ttatcttgat cttcactgac
     8341 gattgtataa tccgcaatca aacgatgagc atccgggata tttaacactt gtttacaaaa
     8401 ctcatataag ttttctactt tttcattgtc tttcgttcgc caactaaata aaaacttagg
     8461 gtgcaagtct ttcggtaagg catttgcaaa ataccgagtg gtttgttcat tggaaaccac
     8521 aaacaactgt aaggtcgaaa agatattgtt ccgaaacata ccttcttctg catatttttt
     8581 gatttggtta aatgcctgat aaacaccatc ttttgcagtt acttgtttca gctcgatttg
     8641 gacaatcggt aatccattga ttaataaggt gacgtcaaaa cgtcgatccc gtgcttctat
     8701 gttacttcca cgttttgcaa tttgatggac cacttcataa gtagagattc ctccaccaat
     8761 gtcttgattg gaataaagga taagtgagac agaacccaat tgcgaatctt cccgttcaat
     8821 cgtgatccga gccatcccat tttcaccttt taaccatttc gcagcatcaa atggggtttt
     8881 cgttcgcagc agtaattccg ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc
     8941 attcagttct gcttgattat ttgagataat tttttggcgt aaattgaccc aaaggtcttc
     9001 ttctgatttt agatccggac gatacgtcca ctgattatgc ccttctccaa gtacacgaat
     9061 caactgttct tcgacttctg cttcctcacg gtgactaatt ttcattcttc ttccctccta
     9121 gacaaacatc ttttgtaaga agcttttttt cgtttctttt agtaaatcta acttacgttg
     9181 atgaagagcg atcgtatcat ctagctgttt gaagaaagta ccgatttttt gttgttcatc
     9241 ctttacagga accttaacac tgatattctc aatatcagaa ctgttataag aaggcaatgc
     9301 ccctacttgg gagtatcttg gcaaatttat agtttcaaac aatattttac caaaatagat
     9361 atcccaagta ttattaaata aataagccat ggtattgtta tctattaaga aagggcttgt
     9421 gaccaaacgt tttctattaa gcattattgc tgcgccaact ttagcaaata taatcgctgg
     9481 cacatctttt attggcttcc attttttacg ctcaatttgc tcattagata cataatgatt
     9541 tgcatttctc atttcatact cattaccaat gttattcatg tcggaaactt taaaaaatgg
     9601 agtaccttca gaaccacctt gctcggcatc aggaaatcca attcccgatt gagttttacc
     9661 taattctccc aacttacgct cttcccaatc ttccgtaaaa ccaggaaacc ggatttctgg
     9721 cacttttgct ccgttttcag ggaacatttt ttgtaagaat ccttttttcg tttccttcag
     9781 caaatctaac ttacgttgat gaagagcgat agtgtcgtcg agttttttga agaatatgcc
     9841 gattttttgt tgttctttta aatcattggg aatatagaat actgtatctg aaactttttt
     9901 ccagtctgaa cgaggcattt ttgttccaga tgtatcattt gcagcctttt gatatctatc
     9961 agcttgaata agagaataaa taaagtccga gtcgctcttc ttagctctaa aaacccagaa
    10021 atccccaatc gctacgcctg taaaagttgc cttgagccaa ttttttaaat atggacgtaa
    10081 ctttccatat agaatatcat ttggtaaaaa ttcaatccct tttctattat caaatttctg
    10141 gctcacatct ttattcagtc taccttctcc tgccacgata tcttcaaatt caacttttgg
    10201 aagtctactg gagtttgtac ttttattaag tctaacaact aaatcaatta acttacgctc
    10261 ttcccaatct tccgtaaatc ctgggaaacg gatttctggt cgtgtatcat tgctcatgtt
    10321 tgaacacctc cagcgcacct tgtaaccaca tttcgttttc aggtgttgtt tgcagggaag
    10381 agatagcttc taaaagttct ttttctagca cttgctgttc ttgtcgtatc ttcttgatgt
    10441 catttccgac atgaaccata tcaattggtt cttcttcttc gaaagtatct acatagcgtg
    10501 ggatatttaa gttatagtca ttttctttga tttcatcaaa ggttgccaaa tgagcgtatt
    10561 tctcgacatc ttttctttca gcgtaggttt cgaggatttt ttgaatgttt tcttccgata
    10621 gtttattttg atttttccct ttaacaaatt cacgactagc atcaatgaat aatacatctc
    10681 gagtttgacg attcttcttc aacacaatca ccgtcgttgg aatcgatgtc ccaaagaaca
    10741 agttcgcagg cataccgatc acagcataaa tactgccatc ttctagtaat ttttgacgga
    10801 tgaccccttc tgcagcgcca cggaataaaa ctccgtgtgg taagacgatg gccatggtac
    10861 ctgtttcctt caaatgatag aacccatgaa gaagaaacgc aaaatctgct ttggattttg
    10921 gcgctaattt cccataacga ttaaaccgag aatcatctaa gaaagtcgta tctgctgacc
    10981 aattagccga ataaggtggg ttcattacta ctgcatcaaa tgtgtaaggt tcatctgttg
    11041 gccagtcctt attcaacgta tccccattgc ggagattcat ttcttcggcg tcgacaccat
    11101 gtaaaatcaa gttcattttt gctagattgt atgtcgttgt atttagttct tgcccatgat
    11161 atttaacgtt atctgggtgt gttaagtagt tacgtacgtt taacatcaat gaacctgaac
    11221 ccatcgttgg atcaaaaaca ctaaagaatc gtcgttcctt ttgatcgagc gttacgattt
    11281 gcgccatcat atcagaaacc atatgaggtg tgtaaaattc tccggctttc ttccctgctt
    11341 ctgaagcaaa ttgactgatc agaaactcat acgcatcccc gatcacgtcc ccatcgtgtt
    11401 ctaaaacatc tacatcattc aatttcttga tgacttcggt aatcgtcaca tttcgttgtt
    11461 gctcatctgt tcctagtttt tttgattgga gatccacatc atcaaataac ccattaaatt
    11521 gattgtaggt agatgccaat tgcacaaagg ctttgtttag atcgttcaat tggaacgttg
    11581 cttgttttgc ttgatccgct aagacgttga aaagatactc tggtgcaatt gcatatccca
    11641 aaatatcaac gattgtggca atcagatctt cttttgattc ttcatctgaa agtaattcac
    11701 gatacaacat tgtttgtttg cttactgtgt catattcctc taaggattca tctgctaaaa
    11761 gaaccacttg ttccaacaat cgatcggata aatatttata gaaaatcagt cccaataaat
    11821 aatttttata ttctgacgca tccattttac tacggagatt atctgccgca ctaaataatt
    11881 tttgatttaa ttctgcaccc aaaaagatac actcctatct cttatttaaa gttgctatat
    11941 accctcttat tttagcatac ccccttttat aaaaagcaaa aaaaaaagaa agaaacttat
    12001 tcttgctaat aaactacgcg aaaggagtct aacgttgttt gatataactt agactgatcg
    12061 ttaacttatt ttttatattt ctacatagtt atctttagaa ccatttcaga tacatatgaa
    12121 acaattacat atgatttttt cataaaaaaa cagtactgtc tagtagtata aaatatacaa
    12181 atttcttaaa aattttttca ctaaaatcac tacctgaaac agtctaaaac acttatatat
    12241 caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc
    12301 aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg
    12361 gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata
    12421 acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa
    12481 tctttttacc cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact
    12541 gcattactga tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa
    12601 atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt
    12661 ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta
    12721 tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta
    12781 actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt
    12841 ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc actataacca
    12901 gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca
    12961 tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt
    13021 cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt attattaatc
    13081 tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact
    13141 tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca
    13201 ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca
    13261 gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc
    13321 catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg
    13381 ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat
    13441 tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc
    13501 aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac
    13561 cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc
    13621 aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat
    13681 agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg
    13741 attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa
    13801 ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat
    13861 tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt
    13921 ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac
    13981 caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc
    14041 ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt
    14101 caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt
    14161 tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa
    14221 ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa
    14281 taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct
    14341 agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc
    14401 ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat
    14461 gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga
    14521 ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta
    14581 gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta
    14641 atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta
    14701 ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact
    14761 gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct
    14821 tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct
    14881 ttaagattca actgatgata caagtgaatt actctagctt gagctaataa aacatggtga
    14941 ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct
    15001 ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca
    15061 ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt
    15121 aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc
    15181 ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt
    15241 tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa
    15301 gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc
    15361 gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt
    15421 tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat
    15481 aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact
    15541 aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta
    15601 attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc
    15661 agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa
    15721 tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc
    15781 aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc
    15841 tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa
    15901 tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac
    15961 cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc
    16021 gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat
    16081 aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc
    16141 aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc
    16201 accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga
    16261 aggctcagta attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc
    16321 tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc
    16381 cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat
    16441 tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa
    16501 aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc
    16561 gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa
    16621 taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg
    16681 ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc
    16741 tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga
    16801 aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa
    16861 ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat
    16921 agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac
    16981 ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt
    17041 ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc
    17101 ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt
    17161 gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt
    17221 ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc
    17281 acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc
    17341 tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc
    17401 acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca
    17461 tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca
    17521 gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac
    17581 atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta
    17641 taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa
    17701 aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat
    17761 ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact
    17821 tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg
    17881 tatcaataga atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga
    17941 tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc
    18001 aaacatatcc ataataattt tttctttttc atttctttta tattgttata acagtgaata
    18061 acataaaaat tcccgacaat cattgtaata tttttttgtc acctgtatta gtatttaatt
    18121 tataggaaac tataaaaact ccctagtgaa tttacacctt tgggagttcc tgtttggacg
    18181 gtatgcataa tacatttaaa tcaatttaac attaacacct cagcatatgg tatctccaca
    18241 tatatacggt ctttctttgg atagtactca atatttatgt tgatagcact tccattgtct
    18301 aatatagctt tagcccggta tgtgttatct ccattttcat gagtaatcgt cacatcatca
    18361 catgatcgat ttaagttgta ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac
    18421 ttctctaatc cagatgtacc tgtcattttt gaaaaaataa tacctataat agccaagaca
    18481 acaataccta taatgacaaa cttcttgttc atatttactt tattcaactt tttatctcct
    18541 atctccaaaa taatttgttc ttatccctct tatcagtaca ttgacatccc taaaagtaca
    18601 ccaaagattg ctccagctac ccctgctatc atcaaaatcg tacccatttt ctcatctttt
    18661 tctcgatata agtagcccat aataacagca acagcaccaa atacaacagg aataaatgct
    18721 aacgatacta ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca
    18781 tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct
    18841 gttccggttg tctcatctgt ttcttgtagt gctccacaaa actggcaata cttactttca
    18901 tctgttattt ctttgccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa
    18961 ttgcttatta gttaactgtt tactttatta aatacatcag aaaacagctc attcattggt
    19021 acacttaata tctcagaaag catcttcatt tgatagggtt taggaatcgt tctccctgtt
    19081 tgccacgaac ttacacattg ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa
    19141 agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc
    19201 tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatataaa aaacttcaaa
    19261 taccattaca aatacaacaa cttgttgtac tatactgaga tgggggtgta ttaatgaaac
    19321 agaaacaata tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg acacaaaaag
    19381 aattagctga aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa
    19441 cagagcctaa cctcgatagc ttaacagctc ttcctagagc cctaggaacc cctgtaagcc
    19501 attttctaga caatgttgtc gtagattatg aggaagagtt tttggctctc tatcgttctc
    19561 ttagcactga agatgcagtt cgaacaattg actatatgaa gctattaaaa aggcaagaaa
    19621 atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg
    19681 ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt taatacaatt
    19741 gggtcttttc tcttccttac tctccgtcca tcatctcttg ttacacttgt aaattgatac
    19801 agaggaacta tcccagctcc tgataatatc aattctttta ttgtttcatc tatccctgcc
    19861 cctttcgcta aatatattaa aaaactacta cttgctttca cattgtatag tttcttaata
    19921 tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttttatt
    19981 aaagcatttt tcacgctgtt atccccaaca ttacgtaggc gttcacaaac agtgttaata
    20041 agttctttct ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt
    20101 gcttcatcta aaatcaattg tttttccttg ctgagttctg atcgggaatt ttgaggaaat
    20161 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
    20221 tatgtaaaat gaattttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca
    20281 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct
    20341 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
    20401 tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta
    20461 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac
    20521 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca
    20581 tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc
    20641 attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
    20701 ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt
    20761 aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa
    20821 taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg
    20881 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
    20941 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
    21001 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg
    21061 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
    21121 tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct
    21181 gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg
    21241 atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct
    21301 tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt
    21361 atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctat gcttaagtat
    21421 ctactatcat ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg
    21481 acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt
    21541 gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta
    21601 acttctctat agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact
    21661 ggcttctcta ataatttttc ttgatctaca taaattggaa tattaatcat tgatgtttcc
    21721 atttgtgttt cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca
    21781 aactctttat tactatatgt tttagtctta tttgtttact caacctttat accgttccat
    21841 gaaactatca atatcagatt ttttaaatct aatgaccgta tcaattttta taacaggtaa
    21901 cccgtattga gaaacatatt ttctaatcgt tcctactgat acacctagat attcacttgc
    21961 ctgctgatag tccatatact ctagagtgac tccagaaata atgtcttcta gcataggctt
    22021 tagcactgta attatctctt tcacttgttg ttcaacttgt atagaaacaa tgctatcaat
    22081 gatttgtaga gacatatttc cccctccttt cgtgattgct tgaacaaatt ttctaagaat
    22141 tatcttcgta ttttttgctc aaaataaaag tattttgtta ttaaccgatt aatcaaacaa
    22201 aaaatgatta ccagttcatc gacatacaca taatacctcg ataaaaaagg ggtgtcaaca
    22261 gctttgtaaa atttatttac aacacccctt ttttgaggta tactaatttt aggaggtgtg
    22321 gtcaatatga taaaacgtcc accaataaat tatttagaac gaaaaaagat actcggaaca
    22381 aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt
    22441 aacaacggtc aattaattga caaaaaaact atctacgaat gggagaaggg aacctattta
    22501 ccaataccag agcgattatc ccgaattgct gatttgggaa atatgagtat agaggagtta
    22561 gtttgtggca atgtagaaga atatattttg ggaattattc tttatagaga tagcattgta
    22621 ctagatggta taacatttcc agataaaaac ctgttccagc atcttcgtca acagttccca
    22681 ccagtacact ctaatttaga tacatggctg gatagatatt ccaaattaga accagagatg
    22741 caagaattta ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc
    22801 aatatcttaa aaatagaaga attattcatc aatgctattg tagaagaatt tgacaataac
    22861 attcttttcc tcactagcag tatagaagaa ctactagaaa gaatggtaga tgaatggcta
    22921 cctattcaat tgaaagacat gtcttatccc gaagaagctg tgagagaaat tactgataat
    22981 ataaacaagc tagaacaaac gatttcatca attgggaaaa aatacactaa gaaaaaaatg
    23041 aagggaggtg ataccatcta gtggtaagta ctccttgtaa actaagagga gatgatttaa
    23101 tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac
    23161 acattgccac cgatccacga actggtgaaa aaataaatac tacgagaaga ggttttagta
    23221 gcaaacaaga agctcaaaaa gattatgacg agtacagaca tcaaatatta tacggtgtta
    23281 aaaagaaagc acctgatatg acatttgaag acctgtataa tgaatggata actcatcagc
    23341 gtaccagtgt taaagtttca acaatcgcaa tctcagtaag atacgcaaat aatcaaatac
    23401 ttccagcatt tggtaagctt aaactatcga atatctctgt cccatattgt caaaaagtgg
    23461 tagatgaatg gcacagtaaa tatgaaagtt acgattatat gagaaaacag actgcacaaa
    23521 ttttacgcta cggggttgca atgcaatata ttgacaataa tcctatggaa aaaacacttc
    23581 ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag gagcttaata
    23641 atttactgga tgcttttaaa gattttggta acatgaaaca atacgccttc ttcagattat
    23701 tggcatacac aggtatgaga aagagtgaag tactcgcttt acagtggaaa gacatagaca
    23761 cgtttaataa agaactacat gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta
    23821 taattcaatc tccgaaaaca agagcttcta gacgagttat ctctttagat actgagactc
    23881 tttctatcct caacaactgg aaattacagc aaaaagaaga gtatttaaaa cttggataca
    23941 atactagctc taaagagcaa catgttttta caacagttaa aaacactcta tacattccaa
    24001 acactgttaa tgattggtta agatatattt tgaaaaaata taacttgcct cgtattacac
    24061 ctcatgggtt tagacataca catgctagtt tattattaga agcaggagaa tctgtcaagg
    24121 tagttcaaca acgattaggg catgaaaata gtaaagtgac attagacatc tatgcacata
    24181 ttacaaataa tgcacctaag aaaacaggtc aagactttgc tgatatgatg gctcatcagt
    24241 aaaagagctg gtcaaaatct ggtcatttct agtatacagg caaaacaaaa aagctagaac
    24301 ccttatatat caagggttct agctcctata acaggtttac ccaacactgc cttccatctc
    24361 ataactgatc aatcgattga gttcgactgc atattccatt ggaagttctt tggtgaatgg
    24421 ttcaacaaag cccatgacga tcatttcagt tgcttcagat tcggtcaggc ctcggctcat
    24481 cagatagtag agttgttctt ctgagatttt tgagactttg gcttcgtgtt ctaaagagac
    24541 ttggctgttg tggatctcat tgaatgggat cgtatcggat ttcgaccatt cgtccatgat
    24601 gatcgtgtca cattcgatat gagagatcga acccgcactg tttttggcaa aggttacttg
    24661 tcctcgatag tttacttctc cgccgtcttt tgaaattgat ttagagacga tagagctaga
    24721 tgtatttggt gcattgtgga tcatttttgc acccgtatct tggatttggt ttgctcctgc
    24781 aaaggcgatg gagagcatgg tgcctcgtgc accttttcca tctaagtaaa cacttgggta
    24841 tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat tcaacggttg cgccttcgta
    24901 tgcttttgct cgtttggtta ctaagttgta cacattgtct gaccagtttt ggatcgttgt
    24961 gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg atggcggcat gcaggctgtt
    25021 acttgaataa gtaggtgccg tacaaccttc gacatagtgg atgcttgctc cttcgtcaac
    25081 aatgatcagc gtacgttcga attgccccgt gttttccgca ttgatccgga agtacgtttg
    25141 taatggaaca tcgactttga cgccttttgg cacgtaaata aaggtcccac ctgaccatac
    25201 ggctgaattc agcgctgcta atttgttgtc ggttggcggt acaagttttg aaaagtactc
    25261 tttgaataat tcagggtatt ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa
    25321 cttttcgaat tcatctttca tattatggta cacgacttct gattcatatt gtgcggaagc
    25381 accggctaga taagctcgtt cagcttctgg tataccaatc ttttcaaatg tatctttgat
    25441 tttttctggt acgtcttccc agtcacgagc gggtttgtca cttggttttt ggtaatattt
    25501 gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat gtttgcattg gcattttgtt
    25561 aaatgtttct agagatttca gacggaattc aagcatccat tctggttcgc ctttgattct
    25621 tgacatctca cggatcactt cttctgtcaa gccttctcct gtactgaaaa ccggttcgac
    25681 atcatcgtga aacccgaatt tatattcttc taattctggt acgcccatgt tctcactcct
    25741 cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt ttccattgct tttcctagtg
    25801 ctttccaggc aagtgtcgca catttgatcc gagcgggaaa tttagctaca ccaccaagca
    25861 tagcagcatc gcctaattgg tcgggatctt tgacttcttt tccttggaca agctgtagaa
    25921 agtctacagt taaatgttct gcttcttcta ttgattttcc gatgacggcg tctgtcatca
    25981 tcgaggcact tgccgtactg atggagcagc cactgccgtc aaaggcgata tttttgatga
    26041 ttccgtcttt tacatcgact tgcaattcga tcacatctcc acatgtgggg ttgttcaatt
    26101 cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg cggatgggaa gaatgatcca
    26161 agatcacttg acggtataaa ttgtctaatt tagatagtcc catgttggaa aaactccttt
    26221 gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag
    26281 ctcgcgcgag cagtcgctga tacttcaagg tacttcaata gcggttgcgc acaatgatga
    26341 cctgctctga cggctacccc ttccatgtct aaagctgtcg cgacatcatg gggatgtaac
    26401 ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg ggtcttgtgg accataaaca
    26461 gtcaatccat caattgcttg taattttggc aacacataag cggctagttc ttcttcatat
    26521 cgatggatat tgtccatacc gagtttttgc aaataatcga ttgcatggcc taatgcgata
    26581 gcgccggcga tatttggtgt ccctgcttcg aatttccaag gtagttcttt ccatgtactg
    26641 tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt caacaggttc catttcttct
    26701 aaccattttc gtttaccata taaaacacca atacctgttg gtccgcacat tttgtgtccg
    26761 ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa tggccatgtg cggggcagat
    26821 tgagcaccat ctactacgat gaccccgccg ttttcatgga caagttcagc taattcttga
    26881 acaggattga agacacctaa gacattggat acatgagcaa tggagacgat tttggttttg
    26941 tccgtaatct ttttcgctgc atcctccata tccaggaagc cttcttctgt tagttcgata
    27001 tatttcaaga cagcacctgt tcgttttgcc aactgctgcc acgggataac attagaatga
    27061 tgttccatgt aagagagcac tatctcgtct cctgctttga tatagcggtc gccgaaggtt
    27121 ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct
    27181 tttgcatgga tgaacgcccg tactttttct cttgcttgtt cataatcatg ggtcgcgcgt
    27241 tctgctaacg tatggacacc gcgatgaaca ttggcgttat cgtgttcgta atagtacgct
    27301 aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg cattatccag ataaactaaa
    27361 ggttcatcat tgacgtcttg aaataagata ggaaaatcat tgcgtagctt tttagcatcg
    27421 atcatccatt caacttccct tcgattactt cgactaattc tttccgaact tgttctacag
    27481 ggattgctgt taagacagaa ccgaggaatc ctcgaatcac taagcgctct gcttcttctt
    27541 tatgcaatcc tctactcatc agatagtaca tttcttctgg atcaactcgt cctacactgg
    27601 cagcatgtcc agcagttact tcgttttcat cgatcaaaag aataggattc gcatcaccac
    27661 gtgctttatc agaaagcatc aagacacgac tttcttgttg ggcatctgca ccttttgccc
    27721 ctttcaagat gtggccgata ccattaaagg tcaatgttcc tttttctcgg atcactccgt
    27781 gttgcaaaat atgcccgatc gtatgagggg ctttattggt tactcgtgta tcgattcctt
    27841 ggatttgtct gcccgagctg attgccacga ttttgacttc tgcgtgtgca ccttctcctg
    27901 ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt catgacacca attgcccagt
    27961 cgacagaagc atctcgtaaa atgtgcccac ggcgattcat ataagaagta atattttctc
    28021 ctaattgatc tacggctgaa tatttgattt ttgaaccagc tttagcgatg acttccacga
    28081 tgatattacc tgatgatttt gctacttgtt ctttggttgt ctggaaacgt tccagataag
    28141 aaaattcact atgttcatct gccacgatca acacatgctt gaagaagtgc tcatcggatg
    28201 cgccatcttg aatgaataag gactcgagtg gttcatcgat cacgacattt ttaggcacat
    28261 acaggaaaat accgctgttc ataaaagcta catggagtgc agtcaattgg tcttcgttca
    28321 tgtttactgc ttttttcata taatattctt ggactagttc tggatattcg atcattgcag
    28381 tgaacaagtc ggtaaaaatg actccttttt ctgctaattc cacaggcagt tgttcaaaaa
    28441 tcgtccatga accttgttgg acaagcacag gattgtcttt cattgcatca aatgccggaa
    28501 tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc aaataacgac cagcgatgga
    28561 atttcacccg atcgatatgc ggcaatggca gttcatctgc tttcgctagt gcgtcttgac
    28621 ggaaggttct catccaatca ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa
    28681 gataatcgag ccagtttttt tctttcattc ttgctccccc ttacgcttct tcttctttat
    28741 agtcgattcc taattcttta ctgataccag cgtagccttc tgcttccaga cgtttagcta
    28801 ggtcagcatt ccctgtcatg actactcggc cttccatcat gatgtgaacg acatcaggtg
    28861 tgatatagtt caataatcgt tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc
    28921 gcatttcatt tactccttta gcaacgactt tcaaagcatc gatatccaga cctgaatcga
    28981 tttcgtctaa aattgcaaat gtaggttcta acatcaacaa ttgcaggatc tcattccgtt
    29041 tcttttctcc gccagaaaaa ccttcgttca gataacgttc agccatttct tctggcatat
    29101 ttagtaaagc cattttttca tctaactttt tcaaaaatgc cataacagaa attttgtcgt
    29161 tttcatcacg tttcgcattg atcgctgcac gcatgaattc cgcattcgtg atacctggta
    29221 tctcacttgg atattgcata gctaaaaata agccaagtcg cgcacgttca tctacttcca
    29281 agtctaaaac atttttcccg tcaaacaaaa tttcgccttc tgttacttca tagtttggat
    29341 tgcccatgat agcggcagat aaggtagatt ttcctgtgcc gttaggtccc atgatggcat
    29401 ggatttctcc ggttttcatt gttaggttta cgcctttaag gatttctttt tcctcgatcg
    29461 acacatgtag attttttatt tctaagacag acatgcatta tacccccaaa ttctctttcg
    29521 ttctcactca ttttagacta attgagaatg aagcgcaatg accttcctta ctaaaatcaa
    29581 atatctttta tttattatta aacaattttg atttctaaat tagaataggt ataaaaaaga
    29641 tgagaaataa gccattacag acttatttct catctttttt gttccatacc ttattttgca
    29701 tcagccttta ctggtttaac agagccgccc catttgtctg tgatccattc ttggacttct
    29761 ttgcggtgaa gcacttcaat cagttttttg attttgtcac tgtcttcatc acctttacga
    29821 gtcgcaataa tattcacgta aggagagttg tccttttcaa gcaatacggc atcttctaat
    29881 ggattcaacc ctgcgccgta agcgaagttt gcgttgattg caactaggtc accttcatca
    29941 ttttggtacg tagaagctaa aagtgatggg tcgatcgtat ggttgaactt caagttctta
    30001 gggttttctt caatatcatc gaatgttgct gtttccagat ctactccatc tttgactttg
    30061 actaagccag catcttgtag aattgtgatg atgcgtcccc agtctgattc agaatttgaa
    30121 gtaatgattg ttgcaccatc ttttaattcg gaaacatctt tgactttttt agagtacaag
    30181 cccattggtt cgatgtggac agcaccggca ttgacaaagt catatccttt ttctttgatt
    30241 tctttgttca aataaggaat atgctggaag taattggcat caatgtcgcc ttcttccaaa
    30301 gattggttcg gtaatacata gtcatcgaat ttgacgattt caagatcgat tccttcatct
    30361 ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact
    30421 ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc tgtcgctgtt gttaccgcat
    30481 gctgctaatc ctacagtcag caaaagcgct gctgcaaatc caaaaatttt ctttttcata
    30541 tcctacatcc ccctatatcc tattatcttt tgtctgtttt cttagttaag aaatcaccga
    30601 ttccttgaac gataaatacg attagtaaaa taattaaagt cgcaacgaat gtcactgtta
    30661 gattaccacg ctggtagcct tcctgccaag caagacctcc aagtccacct gcgccgatcg
    30721 cgccggccat cgcagtaaat ccgatcatcg agatcgttgt aactgttaat ccagaaatca
    30781 atgcgggaat actttcaggt atcaagacct tccagatgat ttcccagtaa cttgctccca
    30841 ttgcatctgc tgcttccagt acaccagggc tgacttcacg aaaagcaatc tctactagac
    30901 gggcataaaa tggtgctgcg gataaaacaa gagctggaat ggctgcttgc gcacctagca
    30961 tagtgcctac gattgcttta gtaaatggaa tgatcaaaac catcaggatc ataaatggcg
    31021 ttgaccggaa aatattactg atgatcgata cgattccata aatgattcga gcatacggtt
    31081 tgtttttgcg tccaatagag taaaggatca atcccaaaac taatccgatg atcgtcacta
    31141 agaccattga gattaatgtc atatatagtg tatcaaggat agcctgattc atcgtttccg
    31201 ggttgatttg tttaaaatct agatatgttt ctgaaaagct tttatccacg atggatcacc
    31261 tcaattccta ctccttgttc tttgatgaac acttctgctt gatctaattc tttggtttca
    31321 ccagtcatca agacagtcaa tgaaccaaag gaaccttctt tcacttgttt gatctttcca
    31381 tacacaacgt tgacatcgat gttatagcgt cgaatcgctt gggaaatgac tggctcgttg
    31441 gcatttcttt cgctaaaggt caaagttgcc aaacggccgc ttggattttc tttgatgagt
    31501 tcagctaata gttctgtcga atcagcttct ggttcgatgt cttgttgtac aaagcgtttg
    31561 gttacttctt ttttcggttg tctgaagaca gtcaatacgt ctccttcttc tacgactttc
    31621 cccaactcca tcacagcgac tttgttgcag attttgcgga tcacattcat ttcatgtgtg
    31681 atcaagacga tggtcaaatt caatttctta ttgatcgcta acaataagtc taatacttct
    31741 tctgttgttt gcgggtccaa tgcgctagta gcctcatcac aaagcaggat ctcaggatca
    31801 tttgccaatg ctcgtgcgat accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga
    31861 tatgcttgtc cgcgtccttc taacccgact aaacgcaaca gttcttctgc ccgttcttta
    31921 cgtttgctct ttggtacgcc agctaattcc agcggcagct ggatattttc aagtacagtt
    31981 cttgaccata acaggttgaa atgctggaag atcataccga ttttctttcg gaatgtacgt
    32041 aattctttgt tttttaattt tgtaatatct tgatcgttta cgatcacttc gccgctcgtg
    32101 ggaagctcaa gtccattcag gagacggact aatgtacttt ttcctgcgcc agaatagccg
    32161 acgattccat aaatatcccc tttttcaacg tttagtgaaa cgtcattgac ggcttggatg
    32221 tttccgtttt tcccattgaa tgtctttttg acattcgtta gttggattaa tgacatgcca
    32281 ttcacatctt tctcttatga tttttcaaac aaaaaagcct tcgccctctt gccttaagca
    32341 aaaggacgaa agcttgaact tccgtgttac caccttgatt taccgttact tcgcaataac
    32401 agcctcttcc agtacttgga cttagcttct ttccgtatac tgtcgcgctg taacgggcac
    32461 tcccgtagta agtttgctta cgctcgctta ctctgctcta agaccatctt ccatctcatt
    32521 ttcgttaccc attttcagca aacagggctc tcttcaaacg tccgtgacat gtactcttct
    32581 tttcaaagct ttctcgtttc atataggatt gcttatgatt atagcaactg gaagaatcag
    32641 tgtcaacttt tttcaagtga ttttctgatt ttataattcg tcaaattctt ttggatcaac
    32701 atctttgaaa cttaagatcc acgcattcat ttcatctgaa tcattcaaga tattgatatc
    32761 ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt agcgggctgg caaattcatt
    32821 gaccgctttt tccgcttcaa cttcgatcag aggttcatct ttttttagcg attgtcctgc
    32881 ttttggcaga ttggcaaacg tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc
    32941 aacacagtat tcttttccat taaataagat ccaaaggttg tcctttttct tcaaatgctt
    33001 gcctgccatt ttatatcagt tctccttcat aaattttttc attgaatcca attgcaacaa
    33061 gctggtcctc tcgtaagatc agtggtcgct tgatcagcat accatctgtc gataagactt
    33121 ggctcgcttt tgaagccgta aactcgccta cttgatcttt cagtccaagt tcccgatatt
    33181 tcatcccgct tgtgttgaac aggcgacgga ttggtacttg actcgtttct agccaagtct
    33241 caatcgttga ggccttcggt acttctttga tcatatcaac ggtttgataa ctaacgccat
    33301 gctggtctaa ccacgctttg gcttttttgc atgttgaaca tttcgggtac cagtaaaagg
    33361 tgtacaattt tcttacctcc ttgaagatct tacaggtgct tcattttggt atctcatcga
    33421 taatatcaac ccgataccga tcatgttccc taaaatcgag gatcctccct gactaatgaa
    33481 aggcaatggg atccctgtta aaggaagtaa tccgatattt gctccaatat tctcaaagac
    33541 atggaacagc agcatcatga tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt
    33601 atcgaaacac acccgaatca tacgatagat taaaataaaa tacaagaaaa tgacgaatgc
    33661 accaccgata aagccgaagt tttcaccgat aacggtaaaa atcatatcgg actcacgaac
    33721 aggcacatag atattgctta cgttgaatcc tttaccaaac aagccaccgg taccaattgc
    33781 caaaagtcct tgggccggct gataactcat tcctgacgta tcatggaaag ggtccagcca
    33841 tgaatcaata cgtgcaaact gataagattt gaatccaact ttataaagca agtctcttcc
    33901 gccttcagtc gtaaccaaga agatcgttcc tgctccaacg actactgcca agattactac
    33961 aggtgcgatg atgcgccaag agattccaga catcaggaaa atcccgccaa agatcgcaag
    34021 aaagacaagc atcgttccaa agtcatcttg ggccatgatc aatgctaaaa ccggtattgt
    34081 cacgatcaag agcttcccaa tcagccaaaa gtccgatttc atcgtccgtt cttttacttg
    34141 cgtattatgt ttagttacga tcagtgccat catcaagatg tacgcaatct tcatcaactc
    34201 tgctggctgg aaagtaaacg caccgaatcg aaaccagttt ttagaccctg ttgaagcagc
    34261 taaattctga tcatagaatt tgagcaagag tcccattacc actaacccca aaccataaag
    34321 gtaaggagtc agtttccaga tccatttgga attcaaatgc atgatgacaa cgattgctgc
    34381 cgctccaact aaataccaga caacttgcat ccctacgcct ttcaagactg ctggattgtt
    34441 cggatcatgg ctaagtgcta catataatga gagcagacca atcaaacata acagaaagac
    34501 tggtaaaatc acgccataat caatacgatt atcgttcgaa gcttttaatt ttttttccat
    34561 gtttcatccc ttttcttaaa aaccatactg ctgattatta tagcggtcca gttagataat
    34621 ggaaagtctt tccatagaca tttgggaaaa attaactttc cgtttgtgca gttgcttgag
    34681 caaatgcagt acaaaagcct actgcttatc tttagtgtat catagaactt tttttgatac
    34741 atgcaattat ctaccagctg agattctcct cgctgtgaga agtttctgtg ctttcattgt
    34801 atcgaaggat ctcttcggca atctctagca gctgatcaga aaccagattg gttcgttgga
    34861 tgaaataaaa atatcgatgg aaattcaacg gagtcattgg cacattttct ggaacagagc
    34921 gcttagacaa aatcgagcga ccgattcctt ccttcagcaa cgcaacaatt acatcgttac
    34981 ttttcacgat catcttctta tcttggatat tcatctcttc aaaataacgc tgcgtgtaat
    35041 gaaaaacacc agagtttgct tctcttacaa gccacaattt gctttttggg tccccagcga
    35101 tgactagctg gtcttccatc aaagagtatc tttgtacgcc ggaagtctct aatggctttt
    35161 cgataaaacc aaaatcggat tcatgtttcg ctacttgttc taatgattca tgagaattcg
    35221 tgatttcgat ctcaaaatgc actttaggaa accttttgat cagatgtttc atcaactgag
    35281 gcaaaagata gacagcaaag gtgtgggaag caacgatccg acatacttct tcttcatttt
    35341 gttcattgag caatttttgc tgagtttctt cccattcttc taacaaaacc agtgtccgtt
    35401 catataataa gtcggcttgc ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa
    35461 gatcgatatt caattcccgc tccaactgtt tgatttgatt cgagactgct ggttgagata
    35521 aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg
    35581 ttaataactt aaacatggca ccacttccat aactttttat gataattact ataaaaacta
    35641 tttgatttaa atatacaaaa aaccatctat aatagaaatg tacaacaaaa cagttgctga
    35701 tccaactagt aacttagatg aagacaatca gctgtctgct tgctcgtaat catacattcc
    35761 tattcatttt acaatgtatt cgttcatctt ggtactaaaa tctattaaat aaaaaggaga
    35821 atattatata tgaagaactt tttgaaaatt attccgatcc cgatctgcgg gttgatcctt
    35881 gggttagctt cccttggaaa tctgtttaaa gactatcatc atgttgcttt aggcaatcta
    35941 actggcggta tctctatgat tcttatgata ttcatttttg ttaaattgtt tatcttattc
    36001 gaacacacga aacaaacgtt gcatgatcca atcatcgcct ccgtttcacc aacttttacg
    36061 atgtcactca tggttatttg tacttatttt gtttcatatg aaagtattgc accaattgtc
    36121 aaattcattt ggctgtgcgc tgttatcttc caagtgatcc ttgtctttta tttcaattat
    36181 caccatgtta tcaaagctga tctttcaatt gaagcaattt atccaagctg gtttatcatt
    36241 tacgttggat ttggggtcat cactgtaact gccgggaact tcttcccact tattggaaaa
    36301 attttctttt ggatttcatt gatctgctat gcgttcttat tgccgatcat catcaaacgt
    36361 gtctttttcg taagaaatat ggctcatccg acacgtccgc tgattacgat cgttgcagca
    36421 cctggttctc tttgcctaac aggttatctt aaaaactttg ctgaacctaa tttttatctt
    36481 gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca
    36541 cgtttgttca agcttcgttt ttatccaagc tatgcagcct ttacattccc gcttgttatc
    36601 tctgcaacag ctatatttac tactgttcat tacttcaaca gtttaggtat ctatagtagc
    36661 gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt
    36721 cttgctcatt acgtacgttt cttaattaaa gaacatagca aacatggaaa atttgtttct
    36781 aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa agctgttctg catcaagtaa
    36841 taagaacggc caaacaaaaa cagctgttca tgtttttgta ctggttgggc ttattaccgc
    36901 agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa
//
LOCUS       DACEMK010000017        34578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEMK010000017 DACEMK010000000
VERSION     DACEMK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.017"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..875)
                     /locus_tag="IW715_000707"
     CDS             complement(27..875)
                     /locus_tag="IW715_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1021330.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1099..3039)
                     /gene="thrS"
                     /locus_tag="IW715_000708"
     CDS             complement(1099..3039)
                     /gene="thrS"
                     /locus_tag="IW715_000708"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1021331.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3376..3555)
                     /locus_tag="IW715_000709"
     CDS             complement(3376..3555)
                     /locus_tag="IW715_000709"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1021332.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3602..5425)
                     /gene="cadA"
                     /locus_tag="IW715_000710"
     CDS             complement(3602..5425)
                     /gene="cadA"
                     /locus_tag="IW715_000710"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1021333.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5703..7982
                     /locus_tag="IW715_000711"
     CDS             5703..7982
                     /locus_tag="IW715_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1021334.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8075..8722)
                     /locus_tag="IW715_000712"
     CDS             complement(8075..8722)
                     /locus_tag="IW715_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021335.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8901..9620)
                     /locus_tag="IW715_000713"
     CDS             complement(8901..9620)
                     /locus_tag="IW715_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1021336.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9751..10902)
                     /locus_tag="IW715_000714"
     CDS             complement(9751..10902)
                     /locus_tag="IW715_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1021337.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11119..11859)
                     /locus_tag="IW715_000715"
     CDS             complement(11119..11859)
                     /locus_tag="IW715_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1021338.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11863..12186)
                     /gene="rsfS"
                     /locus_tag="IW715_000716"
     CDS             complement(11863..12186)
                     /gene="rsfS"
                     /locus_tag="IW715_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1021339.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12243..12836)
                     /locus_tag="IW715_000717"
     CDS             complement(12243..12836)
                     /locus_tag="IW715_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1021340.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12820..13467)
                     /locus_tag="IW715_000718"
     CDS             complement(12820..13467)
                     /locus_tag="IW715_000718"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1021341.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13480..13791)
                     /gene="yhbY"
                     /locus_tag="IW715_000719"
     CDS             complement(13480..13791)
                     /gene="yhbY"
                     /locus_tag="IW715_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1021342.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13807..14916)
                     /gene="yqeH"
                     /locus_tag="IW715_000720"
     CDS             complement(13807..14916)
                     /gene="yqeH"
                     /locus_tag="IW715_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1021343.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14913..15440)
                     /locus_tag="IW715_000721"
     CDS             complement(14913..15440)
                     /locus_tag="IW715_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1021344.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15620..16405)
                     /locus_tag="IW715_000722"
     CDS             complement(15620..16405)
                     /locus_tag="IW715_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1021345.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16398..17267)
                     /locus_tag="IW715_000723"
     CDS             complement(16398..17267)
                     /locus_tag="IW715_000723"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1021346.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17268..18647)
                     /locus_tag="IW715_000724"
     CDS             complement(17268..18647)
                     /locus_tag="IW715_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1021347.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18655..19074)
                     /gene="fabZ"
                     /locus_tag="IW715_000725"
     CDS             complement(18655..19074)
                     /gene="fabZ"
                     /locus_tag="IW715_000725"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1021348.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19099..19575)
                     /gene="accB"
                     /locus_tag="IW715_000726"
     CDS             complement(19099..19575)
                     /gene="accB"
                     /locus_tag="IW715_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1021349.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19578..20813)
                     /gene="fabF"
                     /locus_tag="IW715_000727"
     CDS             complement(19578..20813)
                     /gene="fabF"
                     /locus_tag="IW715_000727"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1021350.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20832..21569)
                     /gene="fabG"
                     /locus_tag="IW715_000728"
     CDS             complement(20832..21569)
                     /gene="fabG"
                     /locus_tag="IW715_000728"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1021351.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21581..22498)
                     /gene="fabD"
                     /locus_tag="IW715_000729"
     CDS             complement(21581..22498)
                     /gene="fabD"
                     /locus_tag="IW715_000729"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1021352.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22605..22829)
                     /locus_tag="IW715_000730"
     CDS             complement(22605..22829)
                     /locus_tag="IW715_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1021353.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22882..23844)
                     /locus_tag="IW715_000731"
     CDS             complement(22882..23844)
                     /locus_tag="IW715_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1021354.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23854..24294)
                     /locus_tag="IW715_000732"
     CDS             complement(23854..24294)
                     /locus_tag="IW715_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1021355.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24600..25565
                     /locus_tag="IW715_000733"
     CDS             24600..25565
                     /locus_tag="IW715_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1021356.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25622..25852)
                     /locus_tag="IW715_000734"
     CDS             complement(25622..25852)
                     /locus_tag="IW715_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1021357.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25938..26825)
                     /locus_tag="IW715_000735"
     CDS             complement(25938..26825)
                     /locus_tag="IW715_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1021358.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26914..28137)
                     /locus_tag="IW715_000736"
     CDS             complement(26914..28137)
                     /locus_tag="IW715_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1021359.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28154..29233)
                     /locus_tag="IW715_000737"
     CDS             complement(28154..29233)
                     /locus_tag="IW715_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1021360.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29364..31091)
                     /gene="ptsP"
                     /locus_tag="IW715_000738"
     CDS             complement(29364..31091)
                     /gene="ptsP"
                     /locus_tag="IW715_000738"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1021361.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31091..31357)
                     /locus_tag="IW715_000739"
     CDS             complement(31091..31357)
                     /locus_tag="IW715_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1021362.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31836..34070
                     /locus_tag="IW715_000740"
     CDS             31836..34070
                     /locus_tag="IW715_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1021363.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34218..34538
                     /locus_tag="IW715_000741"
     CDS             34218..34538
                     /locus_tag="IW715_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1021364.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9766 a         7482 c         6035 g        11295 t
ORIGIN      
        1 aacttatata aaaaatagta aggtttttat ttcgaatcca taaaatattc ttggattgct
       61 tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt tagccatata gaaataaaaa
      121 atgatatcga tcgtagcaaa ttgggccaaa agtgaattag ttgccgcact tcgattcgtg
      181 atttcttttg gtctagatgt ttgtacgagt acatcggcta tcttgctaag cgggttattt
      241 ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt tcgccagctc ggctaatgcg
      301 actacatctg cacttctacc tgagtttgaa acaagccaaa gcactttctt tttctcattg
      361 ctgacaagtt gcgggagcaa gacgtggata tctttttcaa aaatgattgg cttaccgaca
      421 cgagaccatt tttgcagaat atcttctact actaaagaag aggcgccaac cccatataaa
      481 aaaacagtct ctgccgattc caaacactct acgacttttt ggatcgtttc ttcttctaga
      541 atatctactg tttcttttat cgctacttgt gcattgttac caagtttgtt tttgatagag
      601 taaaaggact catttgcttc tatatcacta aaaccttcat cgattccagt ctgtagttct
      661 gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac cgattttttt ggaaaaacgt
      721 acaactgtcg gcgcactcaa cccggaagct tctgctaatt cattagctgt cattgtaggg
      781 accttctcaa tattaccgat aatgtagttg gccaactttt tttctgctga agaataatct
      841 tccatcatac tttcaatacg actaattacg ctcataagtt cctccaccct tttaatgata
      901 gtctatgtaa gtataccata tcagaccctt attcacggtc gtatactgga taaaaaacaa
      961 aaaaacaatc cgcccaacag aatatcttat cccatcaatc ccacagattc tattgagcat
     1021 ctgtgtcttt gaggaatacc tgttatctat tgttcggatt gctttctatc tactactttt
     1081 ttacaggaaa tttacctatt agttttctct gctgtaattt ttgacttctg cttccatagc
     1141 atcaataaag atattcaaat cttctacagc tgtttcttta cttccataac gacgtacgtt
     1201 cactgttgca tcttccaatt ccttgtctcc aacaactaat tggtaaggga tcttttgtgt
     1261 ttgagaggca cgtatcttat agcccatttt ttcattacga tcatcaactt ctacacgcaa
     1321 ccccttcatt tgcaaacgtt ctttgatttc ataagcataa tcgccatgtg catcaacaga
     1381 aacagggatg attgttgctt gtataggtgc caaccatgtt ggaaaagcgc ctttgtatac
     1441 ttctgttaaa taagcgacaa aacgttccat tgtagaaaca attccgcggt ggataactac
     1501 tggacgatgc gtattttccc cgtcttctcc aacatatgtc aaatcaaagc gctctggcaa
     1561 taagaagtct aattgtatag ttgataatgt ttcttctgta ccaagagctg ttttgacttg
     1621 aacatctagt tttggtccgt aaaaggctgc ctcgccttct gcttcaaagt aatctagttc
     1681 taattcatcc atagcggctt tcagcattgc ttgggctttt tcccacattg cgtcatcatc
     1741 aaaatattta tctgtattgt ttggatcgcg atagctcaag cggaaacgat aatctgtaat
     1801 attgaagtca gcatatactg ccaccatcaa ttcaagcgta cgtttaaatt catctttgat
     1861 ttgatcagga cgaacaaatg tatgaccatc attcaacgtc atttctcgta cacgttgtaa
     1921 acctgacaaa gcacctgatt tttcatagcg atgcatcatt cctaattcag cgatacgaat
     1981 tggtaattca cgataagaat ggatcgtgtt tttgtagacc atcatgtgat gagggcagtt
     2041 cattgggcga agaacaagca tttcgccatc tcccatatcc attggcggga acatgtcttc
     2101 ttgataatga tcccagtgtc cagatgtttt gtacaactca acatctccca ttattggtgt
     2161 atatacatgt tggtaaccta gactaacttc tttgtctacg atataacgtt cgatcgtgcg
     2221 tcggattgtt gccccttttg gaagccagaa tggcaaacca gaacctactt ctggggaaac
     2281 catgaacaag tcaagttctt ttcctagttt acgatggtct ctttcttttg cttcttcacg
     2341 gagacggata tattctttca aagctttctt gtcaaaaaag gctgtcccgt atacacgttg
     2401 catcatttga ttgtttgagt ttccacgcca ataagcacct gccactgata ataatttaaa
     2461 tacttgtata cggccagtgg atggaacatg tggtccacga cacaaatcga tgaattcgcc
     2521 ttgttgataa gcagtaatca cttcttcttc tggcaattca ctaatcagtt ctactttgta
     2581 cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa agaacacggc gttcgatcgg
     2641 cagattttct ttgacgattt tcatcatttc tgcttcgatt gccggcaaat cttctgcagt
     2701 tacttggttt tgtccattat ctgtatcgta gtaaaaccct gaatcgatag ctggtccaac
     2761 gccaaagtgg atatttggat agaggcgacg catggcttgg gccatcaaat gagctgctga
     2821 atgacggact aagccaagtg catcttcatg gtctggtgtg atgatctcaa tacttgcatc
     2881 ttcctcgatt ggacgagtca aatcaacgag ttttccgttc actttaccag ctaaagcttt
     2941 ttttgctaag ctttttgaaa tgctttcagc aattgccaat gttgttgttc cagactcaaa
     3001 ttctttgact gcaccatctg gaaaagtaat ttttatcata atagtttcct ccttgaaatg
     3061 ttttgattga tcagatcatc aagctgaaaa acaaaaaaat cctagcatct cttgaaaaga
     3121 gatactagga cgaaaagttc gtggttccac ctaatgttca aacgcaaatg cgttcctcaa
     3181 gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt tcgaaagtgg tcatcctttg
     3241 gttttctcct ggaaaagttt cagcctagct tttcctctct tatcgaagac tctcaaattc
     3301 agttggcttt ctcatcaatc aattatgtgt ttatttaacc actaataaat agaaaaatca
     3361 agtgggaaac taaacttatt ttttccattc cccatttaca ccgtatgttc cttttttcat
     3421 ctcattgatg atgacatgga tgtgttcttt tggtgcctgc gtgtttttat gaacagcttc
     3481 cgtgatatct ttcatcatat tttctaattg ttccggactg cgaccttcta ctaattcgac
     3541 atgtacgaat ggcataatgc tctctcctct ttctgatcgt tcttttttca actcgattct
     3601 atcatactat gtttgtcttg ggtactgaat caggaaaaat ggctggatct ttttttgctc
     3661 tcaaggcact caaaatagag accgtatcca cgacttcttg caaaacagct ccgaataatg
     3721 cggggattag tcccgtactt gcaattaaca tcaaaaaaat acaaataaag atgccaatca
     3781 gtacagactg tttcgctatt ctcatcgtat ctcttgaaat cactactgct tttgctacac
     3841 gcaagagatc atctttcaag atgaccgcat ctgcactttc actagcagct gtagaaccat
     3901 gagcccccat tgcaataccg atatctgcag cagcaagtgc tggtgcgtca ttcacgccgt
     3961 ctcctaccat cgcaacaggt ctttgatctt tagggatact ttctaaaatg gtcagtttat
     4021 cttgaggcaa gctacgggca tgtacagaat ctaattggac tttttctgca atcgtttgag
     4081 caatggcaga tccatccccg gtgatcatca tcaaattttt cacatcaagt tttcttagct
     4141 ccttgattgt ctttgctgct tctgggcgaa tctgatccga aaataggatc gttcctgcaa
     4201 aagatcgatc aattgttaca tagattgctg tattggacgt ttctatttct tcagctgaaa
     4261 caaaagaggc acgtccaatc tttacttcat gattattgac tactgcttgg attccttgac
     4321 cggtcacttc tttcagttga ctaacaggat acagttggct gtttttcacg ttgttcacta
     4381 acgaacgagc taagatatgg ctagattcct gttctgcact agctgcatac agaagtagct
     4441 cttctttttt tattccattg actggctgaa tcgcctctac ttccaatatc cctttggtaa
     4501 gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc gagcttttca atcgttgttc
     4561 cagtttttac aacgatcccg tttttacttg aacggctcat tcctgcaact aaagcaatgg
     4621 gcgcagctag aatcagtgga caaggtgatg ccactaccag tacctcagca aatcgtaaag
     4681 ggtcttttga gaaccaccaa gaaataccac cgatcaaata agcaatcaac gtgaaaggga
     4741 ctgcataacg atcagctaaa cggacaaatc gggcaggctt ttcttttgat tcttcgacta
     4801 gtttaataat catttgatac tggctgtctg cggcacgttt ttcagcgatc atttggatcg
     4861 atccgtctcc atttaaagac cctgacataa tctgatctcc aggtttcttg gtttccgggc
     4921 gggactcacc tgtcaaagaa gattcatcga ccgaagattc tcctttggtc actcgaccat
     4981 ctgcgggtac aagttcgcca ggcttgacta gtaactggtc atcaatattt atttcatcga
     5041 ttactatatc ctgcaatttt ccttctttga atcgatgggc aattcttggg gtgttatcta
     5101 acaaagactg cagttcttta cccgctcgtt tattcgcgta atcctctaga ctatctccgc
     5161 ccgtaagcat gatcaatatc atcaaactag cccagtattc tttgacggct aatgttgcaa
     5221 ggattgcggt aatcgctaat acatctacgc catatcttcc tgaacgtaac gtcttgatca
     5281 tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa tgcgacaatc gcaaatgcaa
     5341 gggccggctg gtgaaaaaca aattcactta ataaagctat cccaccaata agcaaagtaa
     5401 gtaaaaattt tttgaaatgt gtcattgttt tttctcctct tttatgtgac ttagagaggt
     5461 tagtaataaa tttccagctg cctctttcat cttaagcata gtccttgact agcacttttt
     5521 aaaacaatag gccttaattg aaaacaacta tcactatcag cgtttccaaa attcatcagt
     5581 tctacaaaca aaaatattca gcaaggatta ctttttcgat tcatcagtta aaaagtacct
     5641 tttttcgaaa aactatgcta tacttttaat tcgttattta cggcggaagg agcaaacatg
     5701 cgatgaatga gaaacaacta gaacaaacgc acgtggatga aacgatcgaa ctaatccaga
     5761 tcgaacaaaa tatgctggat acaaaacaac aagaattgac tcaggaaatg aatcattcga
     5821 caaaagacac agctaatcac aagattcgag ctggttccaa tgaagctttt tatgaatctg
     5881 ctgttgaata tcgccaacat gagcaggaat tgcttttgaa ataccatact cttgaagcac
     5941 agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag tccttatttt gcccgtatcg
     6001 actttaaaga agaccgtgaa acagaaacat tatatctagg tatcgcttct ttaagagatc
     6061 tggcagaaga aacaatcgtt attgactggc gcgcaccaat tgccaatctt tattatgaag
     6121 gtgagattgg tcccgcattt tatgaaactg aaacagatag aatagatgtc gatctgcagt
     6181 tgaaacgaca atttaaaatc caagaaggac gtattttatc tatggtagat acgtcggaag
     6241 taatcaatga tgactttttg ctagagatat tggatgaatc atcaagcaac catatgaaaa
     6301 acatcgtctc tacgatacaa aaagcacaaa atgcaatcat tcgggacaca acaagtaaag
     6361 taatgctgat tgaaggaatc gccggaagtg gaaagacatc cgctttgctt caacgtattg
     6421 cttttctgtt gtatcataat cgcaagtggc tcgaggctga aaacgttctt ctattttcac
     6481 ccaaccattt gttttctgat tacatctcga ctgtattgcc ttcattaggt gaaagcggcg
     6541 tgcctacaca gacattcaaa aattatttgg tgcagctgct gccagagttt tctttactag
     6601 atgaacagca acaagaaagt ggttttttgc taggggaaaa agatccgata caagtgatga
     6661 aaagcggatt gaccttagtc gatcagattg atcgctatat ccaaggcatc acaaactacg
     6721 gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat cctatcaaag gaatcgattc
     6781 gccaatggta taaagaaaca aatgaacaat tacctttaca tcatagactt tctctattac
     6841 aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt cagcaatggg
     6901 tcaaagatct ggcggaagaa cagctccaag aactatacgc aacagatcca aatcttgaat
     6961 atacagaaca aaaggagcgt tcattacgta aaaaactggc caaccaaatc gtacgtaaaa
     7021 gattccgtcg aatccatcga gggatcatga attatcaatt tgtcaatata acgaaacaat
     7081 atctccactt tctacaaact gtgccacttt ctcttctaac acgacataaa atcagcgaga
     7141 ccaggtggca ggaacatttg acaactgttc gtacacactt gcgtcaaaga gaattgcgtc
     7201 aagaggatgc tgtactattc tttttactga tgcgccgaac atatccagtg acggttacac
     7261 acaaagctcg ctatatcttt attgatgaaa tgcaggattt cgcaccatca caagtggcat
     7321 tgctgcaagc cttgttccca aaagcaaatt tgactttctg tggagatcta aatcaaaatg
     7381 tctttggaaa tgaaacgatc gttggatcat tggaagacct tttcgtggac cgtgaagtca
     7441 ctcgtttcca attaacgaca agctatcgct ccacaaaaga aatcactgat tttgctaatc
     7501 attttattgt gaaagaacat caagtagaaa caacagcaag aaaaggagac cttcctttgc
     7561 tcatccaagg aacaacagat gatatcaaat tgaattggtt agcagatacc attcgaacga
     7621 ctcagggaaa agcccggtat tggcgtacag caatcatagc taaaacacaa gaagaatgcg
     7681 aagctcttta tgctcaactg ccagacgagt tgaaacaaca agtccagctg attttaaatg
     7741 agagtgactt tatgaagcgt tccattattt tacttccagc ctttttagca aaaggtctgg
     7801 agtttgaccg agttttcttg tgggatgtag atgcagaaca tttttcttct cagcaagaca
     7861 aattgatcct ttacaccatg tgtacacgtg cgatgcatga attgaccctt tttacctcca
     7921 ataatgaaag tcctttgtta aacaatatgg atatttcatc ttatgagaaa aaagtactgt
     7981 aagtaaaaag acaactgcta tcattaatgt tagcagttgt ctttttattt acaataataa
     8041 ttgcagaaaa agtaattcaa tcatttaagg tcatttattc tatatataaa tccaaatctg
     8101 tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg
     8161 gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt
     8221 aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc
     8281 cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt
     8341 ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc
     8401 tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg
     8461 ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg
     8521 gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc
     8581 ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag
     8641 ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt
     8701 ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt
     8761 tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac
     8821 acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac
     8881 ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca
     8941 tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat
     9001 agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct
     9061 ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt
     9121 tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg
     9181 atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa
     9241 accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct
     9301 gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca
     9361 aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt
     9421 gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt
     9481 agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc
     9541 ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt tgctacgata
     9601 tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca
     9661 aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt
     9721 ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt
     9781 ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc
     9841 ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa
     9901 gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac
     9961 aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt
    10021 ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc
    10081 agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc
    10141 cagcaaagta gaagataaca accgataacg caaatacggc caataagatt cccaagtctg
    10201 cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt
    10261 tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat
    10321 tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata
    10381 cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag
    10441 cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt
    10501 ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg
    10561 tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca
    10621 gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc
    10681 ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg
    10741 atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc
    10801 aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt
    10861 gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag
    10921 ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg
    10981 aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga
    11041 gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa
    11101 ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc
    11161 tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc
    11221 cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg
    11281 aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct
    11341 gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg
    11401 atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa
    11461 tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct
    11521 atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc
    11581 tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg
    11641 ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc
    11701 ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt
    11761 gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat
    11821 cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact
    11881 aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg
    11941 cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc
    12001 ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc
    12061 tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa
    12121 caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc
    12181 ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct aatatgagca
    12241 gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt
    12301 tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga
    12361 tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata
    12421 tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc
    12481 acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa
    12541 gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg
    12601 tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc
    12661 aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct
    12721 gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt
    12781 tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat attcatacaa
    12841 cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc
    12901 ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca
    12961 aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac
    13021 catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc
    13081 gataataaag taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata
    13141 ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc
    13201 ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc
    13261 aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc
    13321 agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat
    13381 ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg
    13441 aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt
    13501 tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat
    13561 gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat
    13621 ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc
    13681 aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag
    13741 atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct
    13801 tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc
    13861 aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt
    13921 ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg
    13981 aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt
    14041 cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac gattgccgct
    14101 gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata
    14161 aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta
    14221 gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc
    14281 taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc
    14341 ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt
    14401 tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc
    14461 cgtcatctca tgagatttct tagcactagt taataataca tctactggac gaattcctgc
    14521 ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa
    14581 tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc
    14641 tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt
    14701 ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttggat
    14761 ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc
    14821 tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac
    14881 ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata
    14941 tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt
    15001 gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga
    15061 ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct
    15121 aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca
    15181 acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa
    15241 acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct
    15301 gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg
    15361 atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt
    15421 ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag
    15481 tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc
    15541 gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa
    15601 atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt
    15661 tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt
    15721 gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa
    15781 cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg
    15841 cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa
    15901 tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc
    15961 caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat
    16021 ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg
    16081 ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta
    16141 aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca
    16201 gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc
    16261 caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat
    16321 cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa
    16381 cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga
    16441 agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga
    16501 gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg
    16561 atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct
    16621 tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa
    16681 aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga
    16741 gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga
    16801 ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata
    16861 cttcccatga taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt
    16921 gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt
    16981 tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca
    17041 aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat
    17101 ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa
    17161 tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct
    17221 ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct
    17281 atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa
    17341 tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg
    17401 attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc
    17461 gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat
    17521 cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt
    17581 tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat
    17641 tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct
    17701 gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt
    17761 tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga
    17821 aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct
    17881 cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt
    17941 tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga
    18001 tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata
    18061 tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc
    18121 tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc
    18181 atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca
    18241 ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc
    18301 ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct
    18361 tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct
    18421 tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg
    18481 gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca
    18541 tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata
    18601 cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc
    18661 aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt
    18721 gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc
    18781 cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc
    18841 cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat
    18901 caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat
    18961 cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat
    19021 caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt
    19081 ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac
    19141 aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc
    19201 attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc
    19261 tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg
    19321 cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc
    19381 agttgttgct tgttcagata tgacattgac tggctccgaa actggcaacg tttcgtttgt
    19441 ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc
    19501 ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat
    19561 ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg
    19621 ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg
    19681 acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat
    19741 tgatgttcta atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa
    19801 tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg
    19861 atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg
    19921 atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta
    19981 gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg
    20041 tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca
    20101 agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga
    20161 atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca
    20221 atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca
    20281 tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg
    20341 atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg
    20401 gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga
    20461 ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac
    20521 cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg
    20581 tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta
    20641 tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat
    20701 ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg
    20761 tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg
    20821 ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg
    20881 atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa
    20941 cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc
    21001 atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct
    21061 tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc ataatttgct
    21121 tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc
    21181 tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata
    21241 tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt
    21301 ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt
    21361 tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat
    21421 gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt
    21481 gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg
    21541 ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt
    21601 catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca
    21661 taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt
    21721 ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc
    21781 ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag
    21841 gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa
    21901 tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt
    21961 ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag
    22021 gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct
    22081 cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggtag
    22141 cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg
    22201 cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga
    22261 tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct
    22321 gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt
    22381 agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat
    22441 cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt
    22501 ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat
    22561 tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt
    22621 tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg
    22681 tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag
    22741 tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg
    22801 atgatttctt gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt
    22861 ttatttttat aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc
    22921 gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac
    22981 tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt
    23041 ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag
    23101 gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt
    23161 gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata
    23221 cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa
    23281 tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt
    23341 cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga
    23401 ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca
    23461 gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat
    23521 atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg
    23581 tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag
    23641 atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt
    23701 tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc
    23761 catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc
    23821 agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg
    23881 caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat
    23941 gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt
    24001 cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc
    24061 tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt
    24121 ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc
    24181 tgtgattgac agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat
    24241 atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca
    24301 ccgtccacta attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt
    24361 ttcaaaatgc aaccttaaaa gatcattttt ttactatatg agcttttttt aagattttac
    24421 tcatttaaaa caactgataa atcagcattt ataatttctt ttattagaaa aatcatctat
    24481 ctcacatatt ttgattatca aagtattttc tgtgaacaga actatagaat tccctaaaac
    24541 ttgtccatta tttcaaaatt tgttatagtc tttttattca aagcgtaaaa ggagatccta
    24601 tgttaaaaga aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc
    24661 gagatggtaa ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct
    24721 attttcatca gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag
    24781 attttgattt cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga
    24841 tccaacatta ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg
    24901 tacatattac caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc
    24961 cttttcgtct ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa
    25021 tgccttggtt gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg
    25081 aaatgacttt tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa
    25141 agctactgac ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa
    25201 aagccggtat gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg
    25261 aacaaaagca gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg
    25321 agagtcagaa aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct
    25381 ctggtgacgt ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac
    25441 gtcaattgtc aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat
    25501 tagaaaaaga acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa
    25561 attagttgct tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa
    25621 tttattggat taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt
    25681 gctgatgagt atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg
    25741 tacacttctc tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact
    25801 cgaccatagc ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc
    25861 ctccttttct tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct
    25921 ctaaaaaaac tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt
    25981 ttgatcaaag cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt
    26041 ttcaatgctt gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca
    26101 atctttaaat ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa
    26161 atacgaggag aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca
    26221 atcactttct ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct
    26281 gtcatcgtac ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca
    26341 gaaccatgca atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa
    26401 acggcttctt ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca
    26461 ccggataccg gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt
    26521 tctgccaggc ttggtgtact tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca
    26581 gaaaaaatcc ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt
    26641 gtaaagagaa tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct
    26701 tctgttacta aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct
    26761 gcttctagta gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt
    26821 ttcatttttt cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga
    26881 aacaaggatt ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat
    26941 tttgattcgt tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac
    27001 aaccatgtct tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata
    27061 aagtttagct tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag
    27121 tgctggtgca aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa
    27181 tcggttcaaa taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt
    27241 ataagtaagc ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata
    27301 ataaagtgct acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg
    27361 cagttctgag atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa
    27421 tctggcgtta ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt
    27481 tttctcataa gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag
    27541 tgactccctt aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat
    27601 gtcgatacca gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc
    27661 aataacacgt tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa
    27721 gtattttaca tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc
    27781 atacatcgta tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc
    27841 aagtatccct gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc
    27901 aaccaaataa gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa
    27961 tggtacactt ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt
    28021 tgtaaaaata tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga
    28081 tgtagcaact ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt
    28141 tttcgcctcc aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt
    28201 cattcagtac actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct
    28261 cttttgctgt ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt
    28321 ggatcagtcg gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta
    28381 accagccatt atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt
    28441 ctaaaacaac gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg
    28501 aatacgcacc ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat
    28561 gatcttcttt tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa
    28621 accaaataaa tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt
    28681 tacgttcgaa aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat
    28741 attctctgaa cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat
    28801 tggaaatagc ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag
    28861 gtgttggcgt gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga
    28921 taggagaaag ctggttcgat ccgataaagg aatttcggaa atcttcctct gtagaatgcg
    28981 catgatagac aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga
    29041 ttccgtatgt attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga
    29101 taccgacttc cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg
    29161 caagcatctt ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta
    29221 tatagtataa catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct
    29281 tactgacaaa aacgttttct taaaaaagaa caaaaaaact ggcgtagctc ttttacaagc
    29341 cacaccagct tttgatacgt tgtttatttt acatattcat gaacaagttc aacgacttct
    29401 tccatcatat cgcattcttt caatgcacga tcagcaagtt cagccatttt cgctgtgtcc
    29461 aagcgtttca ttaaactacg cgttttaaga atagatgtcg cactcattga gaactcatct
    29521 agacccatac ctactagcaa tggaacagct gtttgatctc cagccatttc tccacacata
    29581 cctgcccatt ttccttcagc gtgtgctgca tcaattacat ttttgatcaa acgtaaaatt
    29641 gatgggttat atggttggta caagtaagaa acgcgttcgt tcatacggtc tgctgccatt
    29701 gtgtattgaa tcaaatcgtt cgtacctaca gagaagaaat ctacttcttt tgcaaacttg
    29761 tctgctaaga ctgctgctgc tggaatctcg atcatgatgc cgacttggat cgtatcagat
    29821 acttccttgc cttcacttat caatttttgt ttttcttctt caaagatcgc ttttgctgcg
    29881 cggaattctt tgagtgtagc taccataggg aacatgatac gtaaattacc atgaacagat
    29941 gcacgaagca atgcacgcat ttgtgtacgg aacatttcgt cgccttgttc agataaactg
    30001 atacgcaatg cacgatatcc taagaatgga ttcatttcat gaggtaattg cagatatggt
    30061 agttctttat ctccaccgat gtccattgta cgaacaacta caggttttcc ttccatacct
    30121 tctaatacag ctttgtaagc ttcatattgg tcatcttctg ttgggaagtc tggagaatcc
    30181 atatacagga attcagtacg gtacagacca actgcttctc caccattatt atggacacca
    30241 actagatctt ttggtgttcc gatgtttgca gctaattcaa aatgcttgcc gtcagctgtg
    30301 acagtttctg cattttttaa tttttcccat tctgcttttt gtgctgcgta atctgcacct
    30361 gctttttcaa attcagcttt ttgttcatct gtaggatcga tgattacatc gccttcgatc
    30421 ccgttgacag ccaaaatatc gccttctttg actttagcag taatttcttt tgtcccaacg
    30481 attgctggaa tttctaaaga acgtgccatg atagcagaat gtgaagtacg tccaccaatg
    30541 tctgttacaa acgcttttac aaaattgcga tctaattgtg ctgtatcact tggagtcaag
    30601 tcatgtgcaa caacgaccac ttcttcgttg atcattgatg gatttggtaa agtaacgcct
    30661 aataagtgcg ctaaaatacg tttagcaacg tcacgaatat ctgcagcacg ctcttgcata
    30721 taagcattat cttccattgc ttcgaacata ccgatataca tatccgttac ttcttttagc
    30781 gctgattcag catttacgct gttgtcttta atattttgct tgatttgacc taccatttct
    30841 ggatctgaaa gaaccatcaa atgggcatca aatacttgcg cttctgcttc acctaagctt
    30901 tgcgctgctt tttcacgaat ttgctgcaat tcttcagtag atttagctaa tgcagcatcc
    30961 aaacgagctt cttcagccgc agtatcttct actgttgcct tactgaatgt taaatccggt
    31021 tgaactagca ggtaagcttt tgcaacggct acaccgtcac tagcggcaat cccttttagc
    31081 atttcaacca ttattcagat aagccttctt tcttcattgt ttcaacgatt gctgccattg
    31141 catcagcttc gtcagcacct tctgcagtaa tagtaacatc agaaccttga cctacaccta
    31201 aagacataac gcccatgatt gatttcaagt ttactgattt acctttatac tctaagttta
    31261 catcagagtt aaatttgctt gctgtttgta ctaatagtgt agctggacga gcgtggatcc
    31321 cagtttctgc tactacgtga aattctttct tttccatatt aatcggtctc ctttaagtga
    31381 gtgttgtttt tatgtgaggg ttttcttttt tagtaattca gagaatccta acaaatgcca
    31441 ccctttcatc tgtaaagatt aacatttttt ttcgttagat acaacagttt tcattcaatt
    31501 tcagataaaa gtattcaaat tattccggat tctttttcat ccttttcttc acattttata
    31561 acaaacgtgt ttatttcctt tttcttgtcg ctttctcttc tcctgacgtt tatcattctt
    31621 tcctttgacc ctctttgttt tcatatgaaa cgaattactt gcttcccttt ttatttttag
    31681 tataataaga gtcaaaggtc aggaatggtc aatagagatt tcaccttttc tacctcataa
    31741 atctcttaaa aaactagggg ttattctttt acctcatgaa aaacgagatg taaaatatgg
    31801 atgtacccat ttttgtgaaa ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga
    31861 atgaggcaac gattcacttg tatgccactg tcaatggtca gcggacacaa ctcgattact
    31921 gtcagagctg ttatcaaaaa atcaaaaatc aacaaaatgg aggtctgact aatatggccc
    31981 aaaatgatcc atttggattt ggcagtttgg acgatctttt ccgttccatg tctcgccaaa
    32041 tgcaacagca aggaaattat gagcaaacac caccaacaca atttggcgga aacaacaact
    32101 tcaatggtgg tcagccacct caaggcaata cagaaggttt attgggagaa tacggaatca
    32161 atatcacaga acaagcacga caaggtgata tcgatcctgt gatcggacgc gatgacgaaa
    32221 tcaaacgagt cattgaaatt ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag
    32281 aaccaggtgt tggtaaaaca gcagttgttg aaggtttagc acaaaaaatc gtagatggtg
    32341 atgtaccgca aaaattaatg gacaaagaag tcattcgttt agatgtcgta tctttagtcc
    32401 aaggtactgg tatacgagga caatttgaag aacgtatgca aaaattgatt gaagagatcc
    32461 gtcaagcaga aaatgtgatc ctattcatag atgaagttca cgaaatcgtc ggtgctggtt
    32521 ctgctggtga tggaaatatg gatgctggta atattttaaa accagcttta gctcgaggag
    32581 aattgcaaat ggttggtgct actaccttaa atgagtaccg catcatcgaa aaagacgctg
    32641 ctttagaacg tcgtatgcaa cctgtacggg tagatgaacc aactgtcgaa gaaacgatca
    32701 gtattttgaa aggtttgcaa aaacgttatg aagactatca tcatgtaaaa tatactgatg
    32761 aggccatcga agcagccgca actttatcca atcgttacgt ccaagatcgc ttcttgccgg
    32821 ataaagccat cgatttgcta gatgaaacag gttctaagaa aaacttgacg atccaaatcg
    32881 ttgatcctaa aacaatcgag aaaaaactgc aagaagcaga agaacaaaaa gttttagctt
    32941 ctcgagaaga agatttcgaa aaagcagctt attatcgcga tcagatcaat aaacttcaaa
    33001 aaatgaaaga acgtcagctc actgaagagg aaacacctgt gattaccgaa aaagatatgg
    33061 aaaaaatcgt tgaacaacgg acaggtattc cagtgggtga attaaaagaa aaagaacaga
    33121 cacaactgaa aaacttagca gatgacttga aagctcatgt catcggacaa gataacgctg
    33181 tagatcgagt agcgaaagcg attcgccgaa accgtgttgg attaaacaaa cagaatcgtc
    33241 caatcggctc cttcttattt gttggaccaa ctggtgtagg taaaacagag cttgcgaaac
    33301 aacttgccta cgaactattc ggttcacaag attcaatgat tcgtttcgat atgtctgaat
    33361 atatggaaaa acatagtgtc tctaaattga ttggttctcc tccaggttat gtcggatacg
    33421 aagaagctgg tcaattaaca gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag
    33481 atgaagtaga aaaagctcat ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg
    33541 gtcgattgac agatgcacaa ggaagaacgg tcagctttaa agatacgatc atcattatga
    33601 caagtaatgc cggaactggt aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag
    33661 gtgttactcg ttctgtattg aaccaattaa acaactactt tacaccagaa tttctgaacc
    33721 gttttgacgg gatcatcgaa ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca
    33781 cactaatgct agacgatgtc aaccaaatgc tggcagcaca gcaacttcac atcgaagttc
    33841 ctacgaatgt caaagaaaaa ttagttgact taggctatga tccatctatg ggtgctcgtc
    33901 ctcttcgacg cacgatccaa gaacaaatcg aagatggtat cgctgaattt tatcttgatc
    33961 acccttctat ccatgagttg aaagcaaaac tggataaaga tggaaaaatc atcgttactt
    34021 ctaaaccaga acgtttggct aaagaatcag ccgaagaaac ggctgaataa atccagtttt
    34081 atagcgtgag acggtatttc tttgagaaat gccgtctcat tcttttttca gaaaagtcaa
    34141 aatactgttc attttataaa aaagaaatta taataagaga aacaagcaat taaaaataca
    34201 taaaagaggt gagcgccatg aaattggtaa atgtaacaaa cagccattcc cgtcttgtat
    34261 taaaccagct tgaaaacact gatgcccatc tagtcaaggt ctacacagcc ggaaatacca
    34321 ctattgtcta cactgaagcc cctgaacata atgaaattct gctgatcaat gacaaacgta
    34381 aaatccagcc gaaagaaatc gaagatgcta aaaattattt cttagataaa atcaaagacg
    34441 ccgtttacca cgaggatgaa atcaaagtaa tcgaattggc aggccttgtt gaaatctcga
    34501 ttccaaaaca tgaattaggc gctacattag ccgtgtaaac aaattttgaa cataataatt
    34561 aaagagcgtg ggacaaaa
//
LOCUS       DACEMK010000018        33754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEMK010000018 DACEMK010000000
VERSION     DACEMK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.018"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            556..1263
                     /locus_tag="IW715_000742"
     CDS             556..1263
                     /locus_tag="IW715_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1021365.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1323..1967)
                     /locus_tag="IW715_000743"
     CDS             complement(1323..1967)
                     /locus_tag="IW715_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1021366.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2094..2381
                     /locus_tag="IW715_000744"
     CDS             2094..2381
                     /locus_tag="IW715_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021367.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2489..2809
                     /locus_tag="IW715_000745"
     CDS             2489..2809
                     /locus_tag="IW715_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1021368.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2890..4203
                     /locus_tag="IW715_000746"
     CDS             2890..4203
                     /locus_tag="IW715_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1021369.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4200..4919
                     /locus_tag="IW715_000747"
     CDS             4200..4919
                     /locus_tag="IW715_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1021370.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            5047..5598
                     /gene="raiA"
                     /locus_tag="IW715_000748"
     CDS             5047..5598
                     /gene="raiA"
                     /locus_tag="IW715_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1021371.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5820..8354
                     /gene="secA"
                     /locus_tag="IW715_000749"
     CDS             5820..8354
                     /gene="secA"
                     /locus_tag="IW715_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1021372.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8480..9581
                     /gene="prfB"
                     /locus_tag="IW715_000750"
     CDS             join(8480..8542,8544..9581)
                     /gene="prfB"
                     /locus_tag="IW715_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1021373.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9686..10372
                     /gene="ftsE"
                     /locus_tag="IW715_000751"
     CDS             9686..10372
                     /gene="ftsE"
                     /locus_tag="IW715_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1021374.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10356..11249
                     /locus_tag="IW715_000752"
     CDS             10356..11249
                     /locus_tag="IW715_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021375.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11401..12849
                     /gene="cls"
                     /locus_tag="IW715_000753"
     CDS             11401..12849
                     /gene="cls"
                     /locus_tag="IW715_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1021376.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13186..14043
                     /locus_tag="IW715_000754"
     CDS             13186..14043
                     /locus_tag="IW715_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1021377.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14158..15078
                     /gene="pstC"
                     /locus_tag="IW715_000755"
     CDS             14158..15078
                     /gene="pstC"
                     /locus_tag="IW715_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1021378.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15078..15962
                     /gene="pstA"
                     /locus_tag="IW715_000756"
     CDS             15078..15962
                     /gene="pstA"
                     /locus_tag="IW715_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1021379.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15977..16783
                     /gene="pstB"
                     /locus_tag="IW715_000757"
     CDS             15977..16783
                     /gene="pstB"
                     /locus_tag="IW715_000757"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021380.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16799..17557
                     /locus_tag="IW715_000758"
     CDS             16799..17557
                     /locus_tag="IW715_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021381.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17570..18247
                     /gene="phoU"
                     /locus_tag="IW715_000759"
     CDS             17570..18247
                     /gene="phoU"
                     /locus_tag="IW715_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1021382.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18485..19516
                     /locus_tag="IW715_000760"
     CDS             18485..19516
                     /locus_tag="IW715_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1021383.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19513..20280
                     /locus_tag="IW715_000761"
     CDS             19513..20280
                     /locus_tag="IW715_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1021384.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20380..21948
                     /gene="liaX"
                     /locus_tag="IW715_000762"
     CDS             20380..21948
                     /gene="liaX"
                     /locus_tag="IW715_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1021385.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22248..22565
                     /locus_tag="IW715_000763"
     CDS             22248..22565
                     /locus_tag="IW715_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1021386.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22565..22915
                     /locus_tag="IW715_000764"
     CDS             22565..22915
                     /locus_tag="IW715_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1021387.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23082..24020
                     /locus_tag="IW715_000765"
     CDS             23082..24020
                     /locus_tag="IW715_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1021388.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            24035..24865
                     /locus_tag="IW715_000766"
     CDS             24035..24865
                     /locus_tag="IW715_000766"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1021389.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24909..25934
                     /locus_tag="IW715_000767"
     CDS             24909..25934
                     /locus_tag="IW715_000767"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1021390.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25949..26887
                     /gene="galU"
                     /locus_tag="IW715_000768"
     CDS             25949..26887
                     /gene="galU"
                     /locus_tag="IW715_000768"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1021391.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            27012..27452
                     /locus_tag="IW715_000769"
     CDS             27012..27452
                     /locus_tag="IW715_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1021392.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27455..28057
                     /locus_tag="IW715_000770"
     CDS             27455..28057
                     /locus_tag="IW715_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1021393.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28120..29223)
                     /locus_tag="IW715_000771"
     CDS             complement(28120..29223)
                     /locus_tag="IW715_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1021394.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29433..30434
                     /gene="ccpA"
                     /locus_tag="IW715_000772"
     CDS             29433..30434
                     /gene="ccpA"
                     /locus_tag="IW715_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1021395.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            30789..31184
                     /locus_tag="IW715_000773"
     CDS             30789..31184
                     /locus_tag="IW715_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021396.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            31184..31435
                     /locus_tag="IW715_000774"
     CDS             31184..31435
                     /locus_tag="IW715_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021397.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            31478..31873
                     /locus_tag="IW715_000775"
     CDS             31478..31873
                     /locus_tag="IW715_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021398.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            31870..33126
                     /locus_tag="IW715_000776"
     CDS             31870..33126
                     /locus_tag="IW715_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1021399.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            33138..33461
                     /locus_tag="IW715_000777"
     CDS             33138..33461
                     /locus_tag="IW715_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021400.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            33454..33720
                     /locus_tag="IW715_000778"
     CDS             33454..33720
                     /locus_tag="IW715_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021401.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        11306 a         5809 c         7257 g         9382 t
ORIGIN      
        1 tttgactccc tcatgcgttg gttcgaatcc agctacccca gtataccaca atcgtaagct
       61 ctcgttagtt cgagagctct tttttttgct ttgctacctt gttccttgaa gattctgctg
      121 atttaagata gagttaagtt aactacaaga attggaggga tccacatgaa atggaaagaa
      181 aaagtagacg aagcagctga aaagttatat gatttggtca aatcagaaaa atacaatatt
      241 gaagtgaata ttccgaaaaa aggcggaaaa gcggtccagg taaaatcaaa acgcccaacg
      301 aatcatacaa aaaaatggat ggctaaaaac cgctaataat aacaaaagat ccataagtcg
      361 gcattttcat cgattttagg gtcttttgtt attttctagg ttgaacatat ctaaaaaatt
      421 gtttctgaca ataggagtag gaagaacgat gtgtgtctaa atcctctgtt gatggataga
      481 aacctttatc tttttcagtt gtgaatcagg tatataccag ttataataga agaaacgaaa
      541 ggacgggtgt acgttatgtc gaatcaatta cctgtatata tacagattca tgaccaatta
      601 aaaaatgaaa tcgaacaagg tatatggaaa gtcggagacc gtcttccttc agaaagagaa
      661 ttagcagtga aattcaatgt cagccgtatg acgttgagac aagcaattca aaacttggca
      721 gatgaaggaa tcttagaaag aaagatcggt tcgggtacat atgttgcgcg agaaaaagta
      781 caagaaaaaa tgtctggtgc aacaagtttt acagaaatta tggaatcgca aaatcgagta
      841 ccgtctagtc ggacaatctc ttatttcttg acttcgccaa gctcaagtga aatggaaaaa
      901 ctaaacttga gtaaagatga cacgatcttg cgaatggaac gtattcgttt cgcagatgat
      961 gtccctattt gttttgaagt tgccagtgtg ccacaaaagc tgatcgatgg ctacagcaaa
     1021 gcagagatta ctgaatcgtt gtatcgcacg ctagaagaaa aaggcgggaa aaagatcggt
     1081 ccagcgaatc aaacgattac tgctgtgttg gcttctgaga aaattgctga ctatttagag
     1141 gtaaaacgag gagatgcagt tcttcgtttg cgccagattt cttattttga agacggtacg
     1201 ccgtttgagt atgtgcgtac gcaatacgta ggaaatcgtt ttgagtttta tttggaaaaa
     1261 taaaaaacag gtgatccctg attccttact tagaaataaa agagaatcag aatcacctgt
     1321 ttttatatat gatcagttac cagttgctcg tgataagaaa caggaccctc ttcaaattgg
     1381 acttgtccgt tcagcagctc aatgatggat tctttaaagt ggtcagcact tttttcatct
     1441 acagcacaga tgacgctaac ttgatcagta tattggattt cttttataaa aaaatgttgg
     1501 agttcaagat aattctgtaa ttttcctagt tgcggatacg tgatcatcac agagatctcc
     1561 tgttgcaagg taccttctac gattccaatg tcagacaatg cttgagagac agcatgagtg
     1621 tatgctcgta ttaatccgcc tgtgccgagt ttcgttcctc caaaatatcg agtgactact
     1681 gcagctacat tgatcagttg attttttttg aggacttcca acatcggtac gccagccgtt
     1741 ccacttggtt ctccatcatc gctgcttcgc tggatttcat ttttttctcc aatgatgaag
     1801 gcgctgcagt tatggttcgc tttccaatgt tcttttttta tttcttgaat gaaacttttt
     1861 gcttggtcct ctgattctat tcgtctaaga gaacagataa agcgtgattt tttgatctca
     1921 atctcacttt gtccattttc ttttattgta cgatagcgct cgatcatctt gtcacctctt
     1981 tttttcattt tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt
     2041 tgcgcaatcg ctgtagtatg cgataataaa gacaaaggta ggtggatctg gggatgcagg
     2101 aacgaatgaa aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag
     2161 taacgttgac ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa
     2221 cagcaaggga aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct
     2281 atggctggat aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg
     2341 tgttcaataa attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa
     2401 cgttgacaaa tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg
     2461 tagtttgttt taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc
     2521 ggctggtatg agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg
     2581 aatcgactcg gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa
     2641 agatgtagat gtactgttat taggacctca agttcgcttt atgaaagctg attttgaaaa
     2701 acgtctggaa ccaaaaggca ttcctttaga cgtcatcaat atggctgatt atggtatgat
     2761 gaatggcgaa aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta
     2821 taactatcga acaagcgatc caaaaaatag atcgcttgtt ttttcgtata tttttttagg
     2881 gcgaataata tgaatcattt ttggggtaga aggcttctga cgagagatat taaagcaata
     2941 gactgtgagg aggtaaattt accaaattat gagaagatcg cagcagtgga acgtttgggc
     3001 aactggattc tttgtcatcg ctgtggaatg aggacgtccg tcattgatgg acagcttgca
     3061 gattacggtt atttttgtac acattgtcta tatttagggc gatgcgacac tcaacaagat
     3121 ttatatttgt ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt ttggacagga
     3181 cagcttactg aaaaacaaac ggaaatcgca aagagaatac tttgccattc agaaaaaaga
     3241 catcatctta tttgggctgt aacaggtgca gggaaaacag agatgcttta tcctatttta
     3301 gtggaaactt taaaagctgg aggaagagta gcgatttgta caccgcgagt cgatgtttgt
     3361 aatgaattgt ttttacgcta tcgttatgtt tttccagaag aaaaaatcac gttgctccat
     3421 gggaatacag caacggctta tacctttact tcttttgtta tctgtacgat tcatcagcta
     3481 taccgttttt atcggtcctt tgatttaata gtgatcgatg agatcgatgc gtttccttat
     3541 aacggggatg caggattagc tcatgctgtg caaacagcga ttaaaccaga cggacgattc
     3601 atctacttaa gtgccacacc tgataaaaag ttactaaagg aaatcaagca gacgtttgac
     3661 atccacaaac ttgctttgcg ctttcatcgg cgtttacttc ctgaacccgt cttggttttc
     3721 atgaataatt ggcgacaaaa atgcaagaac aagaagaaga taaaatcttt ggtgcggatc
     3781 attcatcaat taattaaaga aaaccatatc cttttatttt gtccaagtat cacgatgatg
     3841 catcgtttga aagccacact tcaagaggct cttccgagat atgagatcac agatgtttct
     3901 tcaagagaca atcagcgggc ggaaaaagtc cagaaaatgc gagaagcaaa atatgacata
     3961 ctgttaacca cgatgatcct tgaacgtgga gtcacatttg aaagaatatc cgttatcgtt
     4021 ttaggagcgg atcatccggt ttttactaaa tcttctttgg tacaaatcgc aggaagagcc
     4081 gatcgtaaag ggccttatac gaacagtcaa gtatactttt tctatgaaga aaaaactgca
     4141 gctattcgaa aagcttgtca ggaaatcaaa gaaatgaatg aagaagggaa gaagatgctg
     4201 tgaggtgcag ttgctgcagt aaagatatcg tccaaaattt aacattcagc gagattattg
     4261 gtgtttcgaa atacagatta cctcagactt gttcagtttg tcgcgcacaa tttcatttat
     4321 tggaaacaag aggctgttct ggctgtcaaa aaccaatcca tttcaatcta gagagtacga
     4381 aaaagaaagt aaagtctatt cccgtttact gcagtgactg tcaagaatgg aagaagaaat
     4441 atccgaccta tgattttcag caccgtgcct tttttgtgta cgatgaaaat atgaaggcct
     4501 ggctcaatca atacaagttt atgggagata tccgactagc aggcaccttt gcatataaat
     4561 ggacgcagat aaaaaaagaa tacccgacat acatctattg tccaatacct ttatctgaaa
     4621 aaaggcgaac agaaagagga ttcaatcaag taagcgagat gttgaaagca gcaagaattc
     4681 cttttcagat gctactcaaa agagagaagc atctgctggc tcaagcaaaa aacacgcgaa
     4741 aggaaagatt ggagatgcct caaccttttg aacttgcagt caccttttct aagatacaaa
     4801 ataaaaaaat attgattatt gatgatgtct atacaacagg acagactctt ttccacgcag
     4861 cagattgttt gcaggtcgca cagccgaaaa tcatccgaac catttcttta gcaagatgaa
     4921 ttagatgtgg aaatttgata gcgatttaat ttgcaaataa cctagagttc gatataatag
     4981 aattaagaag agggaaagag tggtccttct ccttcttgat tgtggtagac gaaaggggta
     5041 atttttatgt ttagatataa tgtacgcggc gaaaatatcg aagtaactga agcgattcgc
     5101 gactacgttg agaaaaaagt aggcaaacta gaacgctatt tcagtgacgc acccgatgca
     5161 acggcgcatg tgaacttaaa agtttataca gaaaaaacag caaaagtgga agtaacgatc
     5221 ccattacctt atcttgtatt acgtgccgaa gaaacttctc cagatttata cgcaagtatc
     5281 gatttggttg ttgacaaatt ggaacgccaa atccgcaaat tcaaaacaaa aattaatcgt
     5341 aaatcccgtg aaacgggcat gaatactgca gatgctactg ctttattcaa tgaagaggac
     5401 gaaaacgaaa atgattctga attagatatc gtacgtacga aacgcctttc attgaaacca
     5461 atggatagtg aagaagcagt cttacaaatg aacatgctgg gacataactt ctttattttt
     5521 gaagatgcag aaacaaatgg tacaagtatc gtttaccgcc gtaaagatgg aaaatacgga
     5581 ctgattgaaa cagactgatc agccttactt aaataacaac gaaaacttgc agcagctact
     5641 gacagctgcc gcaagttttt ttgagtttcc tcctctttta gatgaaatat gaataaatta
     5701 tgaagttctt actttaatac gcttaagata tttcaattat ctataactaa tgataaaatg
     5761 aaaagtaaag tctacatagt ctggataata cacagaagga aaaggagatt acacaacgca
     5821 tggccaattt gattcgttca cttatcgaaa acgataagaa agaactaaaa cgtttagata
     5881 gcattgcaaa aaaagttgaa gcatacgctg accaaatggc agcattaact gatgaacaat
     5941 tacaggcaaa aactgctgaa ttcaagggca gataccaaaa aggagaaaca ttagatcaat
     6001 tacttccgga agcttttgca gtcgtccgag aagcagcaaa acgtgtattg gggttatatc
     6061 cacaccacgt acaattgatg ggtggaatcg tgcttcacga tggaaatatc cctgaaatgc
     6121 gtactggtga aggtaaaacc ttgaccgcaa cgatgccagt atatctaaat gctctgactg
     6181 gcgaaggggt acacgtagtt accgtcaatg aatatttagc aacccgtgac tcgacggaaa
     6241 tgggcgaact ttataatttc ttaggattaa cagtggggtt gaatatcaac tcaaaatctt
     6301 cggatgaaaa acgcgaggct tataattgtg atatcactta tagtacgaat aatgaattag
     6361 gatttgacta cttgagagac aatatggtcg tttaccgcca tcaaatggta caacgtccgc
     6421 tgaattatgc cattgtcgat gaagttgact caatcttgat tgatgaagcg agaacgccgc
     6481 tgatcatttc agggcaagca gaaaaatcca cagctttata tacaagagca gacaacttcg
     6541 tgaaacgttt gaaagaagaa gaagattata aaatcgatgt acaatcaaaa acaatcagtt
     6601 taaccgaagc agggatcgaa aaagcagaag aaacattcgg tttggataac ttatacgaca
     6661 ttgaaaatac tgcattgacc catcacttgg atcaagcatt gagagccaac tatatcatgc
     6721 ttttagatat cgattatgtt gtccaagacg gaaaagtact gattgttgac cagttcactg
     6781 gtcggatcat ggatggacgc cgttattcag atggacttca ccaagcaatc gaagctaaag
     6841 aaggcgtaga gatcgaagac gaaacgaaaa ccatggcgac gatcactttc cagaactatt
     6901 tccgtatgta caagaaactt gcgggaatga ctggtacagc taaaacagaa gaagaagaat
     6961 tcagagaaat ttataacatc caagtatacc agattccgac aaatcgtccg gttatccgtg
     7021 atgatcgtcc agatttgctt tacccaacat tacaaagcaa gttccacgct gtcgtgcaag
     7081 atatcaaaga tcgctaccgc aaaggtcaac ctgtgttagt cggaactgtt gcggttgaaa
     7141 catctgagtt attatcagat atgctgaatc aagaacgtat tcctcatgaa gtgctgaatg
     7201 cgaaaaatca ctttaaagaa gcagagatca tcatgaatgc tggtcaaaaa ggtgctgtga
     7261 cgattgctac caacatggct ggtcggggaa cagatatcaa gttaggactt ggggtaagag
     7321 aacttggtgg tctagcagtt atcgggacag aacgacatga atctcgtcgt atcgataacc
     7381 agcttcgagg acgtgcagga cgtcaaggag accctggtat gtcacagttc tacttgtcat
     7441 tggaagatga tttgatgaaa cgattcggtt cagaacgaat caaatctttc ttagatcgga
     7501 tgaagatcgg tgaggaagat gctgttatcc aaagcaagat gctgacgaaa caagtagaat
     7561 ctgcacaaaa acgtgtagaa ggaaataact atgacacacg taaaaacgtc ttacaatacg
     7621 atgatgtgat gcgtgaacaa cgtgaagtaa tctataaaca gcgacaagaa gtaatcatgg
     7681 aagaaaaaag cttatccaag ccactaatga acatggtcaa acggacaatc agtcgtgtag
     7741 tagatgcgca tacacaatta gagaaagaaa attggaattt ggaaagtatc atcgattttg
     7801 ccggcagtac tcttgtccat gaagatagta ttactcttgc agatatcgaa ggaaaaacag
     7861 ctcaggagat caaagagtat ctaaacgaac gagcacaaga agtatttaat accaaagcgg
     7921 ctcaactaaa tggtccagag caactgttgg aatttgaaaa agtcgtgatc ttgcgtgttg
     7981 tggactctaa atggacagac catattgatg caatggatca attacgtcag tcaatcggct
     8041 tacgtgctta tggacagaac aatccattag tcgaatatca aacagaaggc tataagatgt
     8101 ttgaagatat ggtaggcgcc atcgagtatg aagtcactcg aatctttatg aaagctgaga
     8161 tccgtcaaaa cgtacagcgt gaacaggttt ctcaaggaac agcttctcac tcagaagacg
     8221 gagatgccaa agaaaatagt aatactagcg cgaaaaaaca gccaatccat gtgaacaagg
     8281 tcggaagaaa tgatccttgt ccttgtggaa gcgggaaaaa atataagaac tgtcatggaa
     8341 aaaatgcaaa ctaatttctg acagcaggat tggtgagaca aattcttttt ttaagaattc
     8401 gtctcacctt ctttctgtta tttgctgatt ctgttagact agatataatg aaaaaagtat
     8461 agtgaaagaa ggaaaaaaca tggaaatatc cgaaattcga aatctcctga cagaaatgca
     8521 ggaaaaggtc acaagcttca ggaggtctct ttgacttaga ccagctggaa gaagacattg
     8581 cggaagcaga agcaaaaatg gctgaacctg gtttttggga tgatagcgaa gctgctcaaa
     8641 aagtgatcag tgaaaacaat gcaaataaag aaacttatga tcaattccat tctcttgctg
     8701 aagaactgga agaaatcgaa ttgatggtgg agatgttgca ggaagaacct gatgcagcca
     8761 tgcaagaaga agcagaaaca cgtatcaatg cgctagatga aaaaatgaag acatatgaat
     8821 tatctatgtt gttagatggt ccttatgatc gaaacaacgc catcgttgaa ttgcatccag
     8881 gggctggcgg aacggagtcc caagattggg gcagtatgct tttacgtatg tatacacgct
     8941 gggcagagca gcatgggtat caagtagaga cactagatta tcaagcaggt gatgaagcag
     9001 gaatcaagag tgtgacccta ctgatcaaag gacacaacgc atttggttat ctgaaatcag
     9061 aaaaaggtgt tcatcgcctt gtacgtatct cgccttttga ctcagcgaaa cgacgtcata
     9121 cttctttttg ttcggtagat gtgatgccag agcttgatga aacgatcgat atagaaatca
     9181 atccagatga tttgaaaatc gatacgtacc gagcaagtgg tgcaggtggt cagcacatca
     9241 ataaaacaga atctgctgta cggatcactc acttgcctac aggaacagta gtagctagtc
     9301 aggcacaacg ttcacaattg aaaaaccgtg agcaagcgat ggggatgtta aaagctaagc
     9361 tttatcaatt agaagtggaa aagaaagaac aagaagcagc tgcacttcga ggagaacaaa
     9421 aagaaatcgg ctggggttca cagatccgtt cttatgtttt ccatccatat tctatggtaa
     9481 aagaccatcg gacaaacgta gagacaggaa atgtccaagc agttatggac ggtgaccttg
     9541 atttatttat tgacggctat ttaaaaatgc atctacaata agaacccatt gttaaattga
     9601 aataaaattg taagattttg caagcatgtt gaaacattgc catgctataa tagctaaaga
     9661 ctgaaaaaga tatggagaga acgccatgat tgaaatgaag gatgtaatga agaagtactc
     9721 aaatggtacg accgctatcc gcaatctctc aattgtcatc gaacaaggag agtttgtcta
     9781 tgtggttggt ccgtctggag ccggtaaatc tacattcatc aaattgatgt atcgagaaga
     9841 aaaagcttca aaaggaactt tgaacgtcgc aggtcatgat ctaatagcaa tcaaaaaccg
     9901 cgaagttcct tatctacgca gagaaatcgg cgtggtgttc caagactata aattattacc
     9961 aagaaaaaca gtatatgaaa atgttgcgta cgctatgcaa gttatcggca gaaaacctcg
    10021 ggatatcaaa aaacgagtaa tggaagtgct ggaccttgtt ggtttgaaac acaaagttcg
    10081 agtctttcct agcgaattat ccggtgggga acagcaacgt gtctctattg ctcgggcaat
    10141 agtgaatacg cctaaagtac ttatcgcgga tgaaccgaca ggaaatctgg atccagaaaa
    10201 ctcatgggaa atcatgaaat tattagatcg gatcaatgcc caaggaacta ctgtcgtgat
    10261 ggctacacat aacagtacga tcgtaaatac gattcggcac cgagtaattg ctatcgaaaa
    10321 cggccgcatt attcgagatc aagcggaagg ggaatatgga tacgatgatt agaacctttt
    10381 tccgccattt attagaaagt atcaagagtt tgaaacgtaa cggctggatg acagttgctt
    10441 cagtcagtgc ggtaacgatc acattgactt tagtcggtgt attcatggca gttatcatga
    10501 acgcaactaa attagctcaa gatatagaag gaaacgttga tgtctctgta ttcgtagata
    10561 tcggtacaaa agacgaagag atgaaaaagc tagaaaaaga attgaatgac ttagatcatg
    10621 ttaaaaaagt tgaattttca agcaaagaac aggaattgaa aaaaatccaa aacgaaatgg
    10681 gtgattcctg gaaattgttt gaaggtgaca ataacccgtt atatgatgtg tatgtagtaa
    10741 gtgctactga tccttcttat acaaaacaag tagcagaaga agcagcagat ttgaagaatg
    10801 tttctcgttc tgattacggt ggagcttcct cagatcggat tttccaaatc gctggtgcag
    10861 ttcggacatg gggattagtt gcagcggcat tgctgctgtt cgtagcgatg tttctcattt
    10921 cgaacacgat tcggatcacg attttgtcta gacagcgtga aattcaaatc atgcgtttag
    10981 tcggtgcaaa aaatggatat attcgctggc cgtttttcct tgaaggtggc tggatcggtc
    11041 tgatcggtgc gattcttccg attttgatca tcacatttgg ttatgcatgg tttttccaat
    11101 tgatcaatcc atcattatta cgttcccatt attcattgat tcatccacaa aatatcgttt
    11161 ggaaaatcaa tcttttaatg gtcggcattg gtgtgatcat tggttcactt ggctcgatca
    11221 tttctatgag acgtttcttg aaattctaat accacaatat aaataaaatg agaaagttgg
    11281 acagtttcgt tccagctttc tttttttatt ggctaggaga atagaaggaa gcggcttttt
    11341 tccctttact ttaaaaaaca acttgggtat tcttaagttg gtagaagaag aggtgaagcc
    11401 gtgaaaatta tcgttgacaa tttttttacg atccttttga ttatgaatat tttgctttcc
    11461 tttatcatcg tttttcgaga aagaaaggaa actgctcaaa catgggcatg ggtgctcgtt
    11521 ttgatgttca ttccagttgt aggatttatt ttatatattt ttttaggaag agggatttcc
    11581 aaagagaaaa tctttgattt gaagatccaa gggaaaatcg gtaagaacct ggaaattgaa
    11641 gaagacaaac aggcattgat gcgagggctt tatcctcatc cgcctactgg acaagtagat
    11701 gtgaaacaat tgatctatat gctcacggta tttgaaagta cgctctatac gactaaaaat
    11761 gaaatcactt tatttacaga tggtcgtgaa aaattcgatg cgctgattga agatatcaag
    11821 caggcagaag accatatcca cttccagtat tatatttatc gcagtgatgc attaggtgaa
    11881 gaagttcgag atgcattgac agaggcggca agacgagggg gaaaagtcag agtccttctt
    11941 gatgcatggg gttccacaca agtttcttcc agcttttttc agaatttgaa aaaagcaggc
    12001 ggagaagtcg catttttctt tcctctattt gttccgtata ttaatcctcg gatcaattat
    12061 cgcaatcatc gtaaaatcgt agttattgat gggaaaatcg gatatacagg tgggttcaat
    12121 gttggaaatg aatatctagg attggtaaag aaatttggtt attggcgaga caatcatttg
    12181 cgtatttacg gagaagcggt gtataccttg caaaaccgtt ttttgatgga ctggaattct
    12241 cagcatacaa aagaagtagg ctattctccc aaattttttc cgtccattca ctctactggt
    12301 gatatagctg ttcaaatcgt gacaagcgga ccagataccg aacatgaaca aatcaaaatg
    12361 acttatttga aaatgatttc gttagccaaa cgggagatat tgatccaaac gccttattac
    12421 ataccggatg gatcgattca tgaagcactg aagttggctc ttctttcagg cgtaaaagtt
    12481 catatccaaa tacctaacaa accagatcat cttctcgttt actgggcaac gtattctttc
    12541 gctgcagagc taatcgagta tggagcaagg atcgaaacat atgagaatgg atttattcat
    12601 gcaaagacga tgattatcga tggtggcatt gtttcagttg gctcagcaaa tatcgatgtt
    12661 cgttcatttc gcttggactt tgaggtcaat acactggttt atgatgaacg aatggcttca
    12721 cgtgtaagaa aagctttttt tgaagactcg aaaatttcta cgcatctcac aaaagaaatg
    12781 tatgaaaacc gaggaataat catcaagatg aaagaaggat tagcaagatt gatttcgccg
    12841 ctcctttaaa acataaaaag gacatgagaa ccatcaacag atggagatct cgtgtccttt
    12901 tctaacgggg ccatttctga gagtacgtca gaaatggaga aagcgtctcg catgagcgaa
    12961 attaactttg gggtatctct tgttttttgg ggaaagcaag aaataagcta atatatgcta
    13021 ttaaaagaga cacatttcaa agccagtata gcatagaaac ttttaatgag atagtttttt
    13081 ttacaattat gagaatttat tttgttattt aatgattttg attcgttttc atcaagtaat
    13141 gaaaatgata aaataaacat actttaggat taaaggagat ttctaatgaa aaaacttaaa
    13201 ttattcttac caataatagg attagctatt cttttatcag gctgttcaaa gtggattgat
    13261 cgaggagaat ccattacggc tgttggctct tctgctttac agcctttagt agaaacagtt
    13321 gctgaaactt accagacaga aaatcctgga aaattcatca atgtacaagg gggcggaagc
    13381 ggaactggtt tgtctcaagt acagtcagga gcagtcgaca tcggaaactc tgatttgttt
    13441 gcggaagaaa aatcggggat cgatgcctca gcattagtcg atcatcgagt agcagttgtt
    13501 gggatcactc ctattgtgaa caaggatatt ggcgtaaaag acatcagtat ggaaaacctg
    13561 aaaaaactat ttacaggcga gatcaaaaac tggagtgaag tcggtggaaa agatcagcca
    13621 gtagttatat tgaaccgcgc ttcaggaagc ggaacccgta gcacgtttga aaaatgggta
    13681 ttggacggag aaacagctgt tcaggcacaa gaacaagatt caagcggaat ggtgcgccag
    13741 atcgttgccg acacacccgg tgcaatcagc tatgttgctt tttcttatgt ttccgatgat
    13801 gtagatacgt tgaatgttaa cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa
    13861 tggacgattt gggcatatga gcatatgtac acaagagatc atccaaaaaa actgacaaaa
    13921 gaatttttag actatatctt atctgatgaa gtacaagata acatcgttgg agaactaggt
    13981 tatatccctg tttctcatat gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag
    14041 taatctttac acaaaattta caaacttagc taaactttta catgttatta aaaccaaatt
    14101 gatgttcaat ttgtacactg agtgagtatg ttaaacctga ttaaggggga gtcatctgtg
    14161 gaagatgtga aaaaagtatt attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga
    14221 aaattcatca gttttttatg tatcgcgctg atcgtgttgg ttgtattatc tattttttat
    14281 tttgtagcta gtaaaggatt ggccaccttt tttactgata agatcaatgt atttgatttt
    14341 ctgtttggta cagattggaa tccaagccaa atcggagcaa atgggaaacc aatggtaggg
    14401 gcattgccta tgatcacagg ttcgtttatt gtgacctttt tatctgctat cgttgccacg
    14461 ccatttgcca ttggtgcagc agtattcatg acggagatct ctcctaaaaa cggaacgaag
    14521 atcttacagc cggtcatcga gctgcttgta ggtattcctt ctgtcgttta tggttttatc
    14581 ggtttatcag ttatcgtgcc ttttgtcaga agtatttttg gcgggactgg gtttggtatc
    14641 ttagctggta catttgtgtt gttcgttatg atcttgccga ctgtcacaac gatgactgtt
    14701 gatgcattaa aggcagtgcc acggcactat cgggaagctt cattagcgct tggtgccaca
    14761 agatggcaaa caatctacaa agtcgtgcta cgagcagccg tgcccgggat tttaacagca
    14821 gtcgtttttg gtatggctcg tgcgtttggt gaagccttag ctatccaaat ggtcatcggt
    14881 aatgcagcat tgatgccaac aggtcttact actcctgctt ctacattgac gtccatctta
    14941 acaatgggaa tcggaaatac aatcatggga acagttgaaa ataatgtgtt atggtcatta
    15001 gcgctgatct tgctattgat gtcattattc tttaatatcg tgatccgtat gattgggaag
    15061 aaaggagagc tgaaataatg aacgcaaaac gctcagataa aattgctaca ggtgttttat
    15121 atatagtttc ggggatcatc gtattgattt tagcagcttt actactttat atcttagttc
    15181 ggggaatacc gcacatttca tggtccttct tgaccgaacc ttccaaagcc taccaagaag
    15241 gcggtggaat cgggatccag ctatttaact cgatttattt gcttttgatc acgatgatca
    15301 tcagttttcc tatttcttta ggtgcgggta tctatctttc agaatatgct ggaaaaaact
    15361 ggctaacaga tgtgattcgc acgtcgattg aaatcttgag ctcgttgcct tctgtcgttg
    15421 tcggtttatt cggtttcttg gttttcgtta ttcaattcca gtatggtttc tcgattattt
    15481 ctggtgcatt ggctttgact gtattcaact tgcctctttt gactagaaca gttgaggagt
    15541 ctttacaagc ggtccattac acacaacgag aagcaggatt ggccttaggt ttgtcacgct
    15601 gggaaacagt gataaaagtg gtggttccag aagcgacacc agggattttg actggtgtga
    15661 tcctaagttc tggaagaatt tttggtgaag cagctgcatt gatctatacg gctggacaaa
    15721 gtgcacctgc gttagatttt acgaactgga atccattaag tgtttcaagc ccgatcagca
    15781 ttttccgtca agctgaaaca ttagctgtcc atatttggaa gatcaataca gaaggcacga
    15841 tgcctgatgg tgtttctgtt tcggctggcg cttctgccgt gttgattatt gctgttttac
    15901 tgttcaactt cggcgctcgt gcgattggca aacgattata ccgaaaaatg acttcagcct
    15961 aaggagaaga agcaaaatga aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc
    16021 aaaagagaaa gaagtagcac tatatacgga tgatctacat gtctggtatg gcgacaatga
    16081 agcaatcaaa ggagtggatc tggaatttga aaaaaataag atcactgcac tgattggtcc
    16141 ttccggctgc ggaaaatcta cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc
    16201 taatacgaaa atcactggga aaatcatgta tcaaggcgta gatattaatg cgcctcaagt
    16261 ggatgtttat gagatgcgca aacggattgg catggttttt caacgtccta atccgttcag
    16321 caagtcgatt tatgacaata ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa
    16381 gaaattagat gaaatcgttg aaacaagctt gaaacaagct gccttgtggg accaagtaaa
    16441 agacagcttg gacaaaagtg ccttggcttt atctggcggg caacagcaac gtttatgtat
    16501 tgctcgtgcg atcgcgatga aacctgatat cttgctgcta gacgagccgg caagtgcttt
    16561 ggaccctatt tcgacaggaa cggttgaaga aacacttgta aacttgaaag aagactatac
    16621 gatcatcatc gtgacgcata acatgcaaca agcagctcgt atcagcgact atactgcctt
    16681 tttctattta ggaaaagtaa tggaatatga caaaactaac aagatattta ccagaccaaa
    16741 aattcaggca accgaagatt atgtgtccgg tcattttggt taagacggag gcctcaacat
    16801 gacaaaagag attatttcat ctaaagatct tcatctgtat tatggaaaga aagaagcact
    16861 aaaagggatc gatctaagct tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg
    16921 ttgcggaaaa tccacttatc tgcgttcttt gaaccggatg aatgacctga ttccgaatgt
    16981 aacgatcacc ggaagtgttt tatataaaga caaagacatc tatggtccaa aaacagatgt
    17041 tgttgaacta agaaaagaaa tcggcatggt attccagcag ccaaacccat ttcctttttc
    17101 tatctatgaa aatgtcatct atggattgaa attaaaaggg gaaaaagata aaaaagtcct
    17161 tgatcaagta gttgaagaaa gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa
    17221 attgcataaa agtgctttat ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg
    17281 tgttttggca gttaatccgg aaatcatctt attggatgaa ccaacaagtg cgcttgatcc
    17341 tgtttctact gggaaaatcg aaagtatgct gttagaatta agagagcggt atacgatgat
    17401 catcgtgacc cataatatgt ctcaagcttc acgtatttca gataagacgg cattcttttt
    17461 agatggtcac ttgatcgaat acaatgatac gaagaagata ttcatgaatc cagaaaaaca
    17521 agagacagaa gattatattt caggtcgatt tggataagga ggaaacggaa tgctacgtac
    17581 acaatttgaa gaagaattgc tgaatctcca taatcagttc tacgaaatgg ggatgatggt
    17641 gagcagcgcc gtgcacaagt cagttcgtgc atacatcaaa cacgataaaa tcttagccca
    17701 agaagttatc gaaaacgata taaatatcaa taacatggaa acacgcttgg agaagaaaag
    17761 ttttgaaatg atcgcattgc aacagccagt tacaactgat ttgcgtatga tcattacggt
    17821 catgaaagct agctcggatt tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc
    17881 cacgattcgc gtaaaagggc agacgagaat accggaaatc gaaaaagaga tctccgatat
    17941 gtccgattat gtgaaaaaaa tggtggacaa cgtcctagtt gcttatgtca aaacagacga
    18001 aaaagacgca cgtacgatcg ccaatatgga tcaaagagtc aatgactatt tcaatcgaat
    18061 ctataatgca acaatcaaaa atatgcagga aaatcctgag acagtcatca gtggaacaga
    18121 ctacttgaat gttgccagtt atctagaacg gatcggagac tatgtgacaa acatctgtga
    18181 atggatcgtc tacttggcta cagggaaaat cactgagtta aatacgaacc acaatgagat
    18241 tttctaaact gaagctgtga ttatgccgtt tgtttaggaa aagaggttgt gaaatcagcg
    18301 gttgtgaaaa agctgttctg catcaagtaa taagaacagt caaacaaaaa atagctcctc
    18361 atatttttcg tttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    18421 gggaaagggt ttgtgacaaa acatgtcaca aacccttttc atcatttcat agaaaggata
    18481 aagaatgcaa aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg
    18541 ggttattcag catcaagccg ctttatatga agtacctgtc atcgagatag aaaaagtacg
    18601 aataaaagag gatcatcaat tgattacagg aaaactgatg aacacggatc aaaaaggaga
    18661 atcgatttat ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc
    18721 gagacaaatg tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa
    18781 tgatggctgg cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggtgg
    18841 aaaacagggt ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt
    18901 gttgctcatc tttactaaaa cagaagggat ttctctatta aatatatcta tcctgttcgt
    18961 cgttataggg atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat
    19021 caaaatttca gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat
    19081 gaatcattgg aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta
    19141 tcgttctgtt tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt
    19201 tatcacagtc attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa
    19261 acagttatgg ctttcaggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat
    19321 ccttttgttc gtctatatca gtgggtcagt tccatcgttt cttttatatt taggaaatca
    19381 atggtcgttc aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc
    19441 tggcagctta ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag
    19501 gaggctgaaa cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta
    19561 cttattggcg gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc
    19621 tttttcctgt tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata
    19681 ggatttttat tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg
    19741 cgtgcagctt ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc
    19801 actgattttt tagcaatcca aggttttaca tcggtagaac tagaagaatt atctggctta
    19861 gataagactt tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt
    19921 agtggtgctg ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag
    19981 gcaaatccgc aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa
    20041 atattggcgt caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg
    20101 atcctctggt ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt
    20161 gttctagaat atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta
    20221 acctgcgtga ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa
    20281 aagttacaac ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg
    20341 aaaagcttgc aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt
    20401 ttagatttag tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc
    20461 atggcaactg aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt
    20521 cataaagaaa aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa
    20581 atttctgagc cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag
    20641 atcttagatg aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat
    20701 gctcaattcg ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg
    20761 aatacaaaag aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta
    20821 gaagcagaaa tcaaagaatt agaagctagt ggcgatacac tgttagaaga acaattgaaa
    20881 ttagaagcag aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt
    20941 actgataagt ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa
    21001 gtaggcgaaa aaatgtcaga agctggatca cagttaggca aattcttgaa gaaaacattc
    21061 caaactgttt cagaaacagt gaatgacaac atggaatgga aagatgtcag cttacgtgtc
    21121 cctgggattg cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt
    21181 atccttgata tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat
    21241 gtgaaagttg aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca
    21301 ttctctgaac ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat
    21361 aaacgtgtgc gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct
    21421 attaaacttc taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca
    21481 aaatctacga atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa
    21541 ggtgtaaacg gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca
    21601 gtaaatggta cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat
    21661 ggtgatgtac gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg
    21721 aacggaaatg tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca
    21781 agtcttggaa gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa
    21841 cggacaaacc aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa
    21901 ctttcaacga caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa
    21961 agagatgttc agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac
    22021 aaaattgtgc cttattgccg atagtctatc tcttgaacac cgtttaccaa gagattatga
    22081 acctgccaat tatcgccgag tcacaaggaa taatttcaga aaaagctcgt cacgttttat
    22141 gaatatggta atttgtgacc gagaattttg cagaaagaca ccgattaaga agaaggttgt
    22201 cacaacgatt ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt
    22261 tgacaaaatc taataaaaat gttgttttga cagggacttt agcaggaata ggagaataca
    22321 tcgggattga tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg
    22381 gaagtccaat tattttatac attttattag ctttgattat cccatctgcg cgttctgcac
    22441 gaacaaacta tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc
    22501 gtgaacaaac aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact
    22561 tttaatgtcg tactttcaac gtattttagt taatacgtta acattcatat cattggctgt
    22621 tctttttcct gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt
    22681 attgtctgtg ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac
    22741 attagtcaca ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc
    22801 cttttttgtg ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc
    22861 tatcttgatg tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa
    22921 agctgtgccg aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta
    22981 cattggtaat taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat
    23041 aaaatgatag agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag
    23101 ttagccaatt agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag
    23161 accgtgtcat cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta
    23221 attattattc ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac
    23281 ggatgatccc agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg
    23341 catttattat ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa
    23401 atgggatagc tgtactgcgt tctcccatct caacttctcg tttattagga gaattatcca
    23461 gttatttaga tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt
    23521 acggattagg tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg
    23581 agttgatcaa acgcggccat cgattgatcg cagacgatcg agtagatgtt tatcagcaag
    23641 atgaattaac ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag
    23701 gaatcgggat cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc
    23761 aagtccagtt ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg
    23821 gctctgaaga cacgaccgtt gaaatcgcgg aagtgggcat tccacaaata aaaatccctg
    23881 tcaaaacagg acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac
    23941 gtacaatggg ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag
    24001 aaaactcagg caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc
    24061 ttttgagctt gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat
    24121 tatcgctatg tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt
    24181 gacagatttt atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt
    24241 tttatttgat cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg
    24301 aggcttagct atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa
    24361 atatcatttt atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc
    24421 acaggcaatc ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac
    24481 gcgaagcttc ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga
    24541 agggatctat cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat
    24601 tttgcttttg tttttacgaa agaaaccaca ccttttgaaa aaaggcgaag tccttttatg
    24661 ttatctaatg tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct
    24721 ttacttttta ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac
    24781 attgattcta tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt
    24841 aggaaaaaat caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga
    24901 gtggtaaaat gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg
    24961 cacaagtcct cgccgaaaac ggacatgacg ttcggatttg gggacatcat gcttcacaag
    25021 tagaagaaat caacacccaa cacacgaatc aacgttattt accagatcat tatctacccg
    25081 attccatcca agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat
    25141 tcgtcattcc aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata
    25201 caaagccagt gatcatccat gcaagtaaag gattagaaca gggaacacat aaaaggatct
    25261 ctgacatctt gttagaggaa atcccagcag aaaaaagaaa agctgttgtt gttctttctg
    25321 gaccaagcca tgcagaagaa gtagcaaaac atgacatcac tacgatcaca gctgcaagta
    25381 cagatgcaaa agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct
    25441 ataccaatcc tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta
    25501 ttggtgctgg cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga
    25561 cgcgcggatt agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct
    25621 ttatcggatt aagcggaata ggcgatctag tcgttacttg cacaagtgtt cattctagga
    25681 actggcgcgc aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata
    25741 tgggaatggt cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaatga
    25801 tggaaatcga aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa
    25861 tcaaaacagc cgcaaaagaa atcatgttac gtgatggaaa aaaagaaaat gaattctcat
    25921 taacaatttt ttgaaggagc gaatgtatat gaaagttaaa aaagcagtta ttccagcagc
    25981 aggattagga acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat
    26041 tgttgataaa ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga
    26101 catcttgatc gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat
    26161 tgaattagaa acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga
    26221 aacgacagat gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc
    26281 cgttcttcaa gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga
    26341 tataatggaa gacgaagtac cattgacaca gcaattgatc gatgactatg agcgtacaca
    26401 cgcttctacg attgccgtta tgcaagttcc tcacgatgaa acttcaaaat acggaatcat
    26461 caacccaggg gaagtactgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc
    26521 agatccaagc aaagcaccaa gtgatttagc tattattggg cgttacttat tgactccaga
    26581 aatctttgat gtattatcag aacaacaacc aggtgctgga aatgagatcc aactaactga
    26641 cgcaatcgat acgttgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg
    26701 ttatgacgtt ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctgacaca
    26761 tccagaagta ggggcacctt tgcgtgaata catcatgtct ttaggtaaaa cattagaagc
    26821 agaaggaagc aagatcaatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa
    26881 aaaataatct ttttttctaa cgagcttcag cggctaattg aataaaaagt agttgaagct
    26941 cgtttttatt tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag
    27001 ggagtttgat catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga
    27061 tacttgtcgt tttcatcgtt cgcgtattgt tgcaagtttc caaaacttta gaaaaagtag
    27121 accagactgt cgcagaagca aatacgacaa ttgagatcgt cacacaagac gtagatcttt
    27181 tatctagaca agtagaaggc ttgctagtca aaagtaatga gctgctcttg gatatcaatg
    27241 gaaaagtcgc aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat
    27301 cggaacttaa tcattctagt aaaaacatcg tgacgaaagt cggcggaatc ggaaaaacga
    27361 cagctaaagc gactgttgcc ggaaaagtcg gtggaacagc gatgaaattt tttaaaaaca
    27421 aaaaacatac tgaaacgaca ggaggaaaat aagaatggct aaaaaaggtg gatttttatt
    27481 aggtgcagta gttggaggta cagccgcagc aatcgccgca ttattgttag cacccaaatc
    27541 tggtaaagaa cttcgggatg aattggctgc tcaagcagat gatttgatgg acatggcttc
    27601 agattacaca gatatggcaa aagaaaaatc atctgagtta acaggtattg caaaagaaaa
    27661 agcgcaaggt ctttctcaac aagcagggga tctagcaggc aatgttaagc agaaggtaag
    27721 tgaatcagca gaggaagcag gaagtgaatc agatgatatc ttgttaaacc tcaaagaaca
    27781 atcagctgat ttatctaatc gtttcaaaaa aacagcagaa gatctgaaaa agccagcaga
    27841 cgaattaggt agtattgcta aagatacttc agaagatatc atgatcgatg taaaagatgc
    27901 tgcagataaa gcaggagaga cggtcaatga aggaatgtca gaagcaaaac cagttgcaga
    27961 tgacacaaaa gaaatgttga aaatggcaaa agaagatgca aaagaagcta aaaaagaaat
    28021 gaaagataca atgacagatt cgactaccaa tcactaagat tactcatttc tagaataaaa
    28081 aaggtgagag cggaccagct ctcacctttt ttattttcat tactctaaaa caagtaattc
    28141 ttttggtgta gtcgtgaatg gcacacaacc atcacttgtc acatacacac aatcttcgat
    28201 acgtacccct actttttcag gaatatagat tcctggttca agagagaagc acatcccttc
    28261 ttcaatcact aagtcgtttc cttctacaag agaagggaat tcgtggacgg ttgttccgat
    28321 tccgtgacct aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg
    28381 tgcgatttga tcaagttcac ctgctgtcac acctgggcga actgcctctt gtgcggcaag
    28441 ctgtgcttca agagtgatgt tatagatttt ttcttggaac tcagttggtt tttgataagc
    28501 aactgtacga gtagcatcac tgcaatagcc attccaaata acgccaagat cgaataacac
    28561 tagttcattt ggtgaaacct tcgtgcttcc tggtgtacca tgagggcttg cagcatttgc
    28621 tcctgcaagg actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt ttaattggta
    28681 ttcgatttct gcgataattg cttgttcagt tacgccttct tttactgttt tgaagccaat
    28741 ttcaaaggca acatcggccc atttaccagc ttcgatcagt ttgtcttttt cttctaccgt
    28801 tttgatcagt tgcagacgct ggataataga agttagatcg gtgtaagaat ctgtacctgt
    28861 caagatatca gagatagcat cgaagcgatc gacagtcaga gcattctttt caatacctac
    28921 attaccaggt tgcccatagc gtgcgcgaag ctcttttccg atgatctccc aaggattttc
    28981 gctgtctaag tagccacgta catcgtacgc ccagctgctg ttttgagcat cttcaacttc
    29041 cagagcagga gtgaaaagga aaggatcttg ttccagaggg ataaaaagag ctagtacacg
    29101 ctcgtgagga tcactttcat atccggaaaa gtaagcgatg tggccagggt cgctgatata
    29161 agccaattct acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt
    29221 catttggcaa cctactttct aaattaattt tgtattcaat aattgtacca ctgattgatt
    29281 aaaagaccaa cgttcttaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa
    29341 cggttgcaat tgttgagatt atttgatatc ctattttaga aatgtaagac gtattttcag
    29401 aaaacatata aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg
    29461 atgtagcgag agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc
    29521 caaacgtcaa accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc
    29581 gtccaaatgc agtcgcacgt ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca
    29641 ttcctgatgt cagcaatatg ttctttgcct ctttagcacg tggtatcgac gatgtggcaa
    29701 caatgtacaa atacaatatc atcttagcaa actcagatgg caacgatcaa aaagaagtca
    29761 atgtattgaa taatttattg gctaagcaag tggacggcgt gatctttatg ggtcaccata
    29821 tcacagacga aatccgtgga gaattttcac gttcaaaaac gcctgttgta ctagctggtt
    29881 ccattgatcc agatgaacaa gtaggtagcg taaacattga ttatgtgtca gcaacaaaag
    29941 atgcagtaaa taaattagca aaaagcggca ataaaaaaat cgcttttgtc agcggtgcgc
    30001 tgattgatcc tatcaatggt caaaaccgtc taaaaggcta taaagaagca ttaagcgaaa
    30061 acaatctacc atatagcgaa ggattgattt ttgaggcaca atacaacttt aaagatggtc
    30121 tgtctctagt tgaccggatc cataatagcg gtgcaacagc tgtctatgtt tcagacgatg
    30181 aattagccat tggtatcttg gatggtttat tagaccgcgg cgtaaaagta ccagaagaat
    30241 tcgaaatcat cacaagcaac aattctttac tgacagaagt agctcgtcct cgtttgacaa
    30301 gtatcacaca accattatac gatttaggag cggtatccat gcgcctattg acgaaaatga
    30361 tgaataaaga agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag
    30421 gatcaactaa ataataaaag atcaatcatt taaaggttgt tccaaaagtg gtcaacttct
    30481 ggaacaacgt ttatacgaga aagggggtta tgacaaaatt ttgtcataac cccttttttt
    30541 ttgtgacttt aaaaaaagag aaaatggcgg aaacaaacct tacgtccatc gtatacgaca
    30601 caatctgtgc gtagatgcta cattttgttc actactatca aaattacttt aaaatgaaaa
    30661 cgtgataaaa aagaaagtag aaaagtagat aagtaactct aaaaaatcca aaaaaatcca
    30721 ggctcgattg cgtacttatc ttaaaggaga aacaatccat catatataac ccgaaaggag
    30781 acttctattt gcctaagaaa agacgaaaga ccatagtcat cattttgacc attcttacta
    30841 taggaatcgg agggaaattt tacatggaca aaagagaaat gcaaagacag aaagatttac
    30901 tcgcagtaga aaaacagtcc gtcaaagttc tgaaaaatac ctttgcggat atcaaggaag
    30961 tgaagattga aaaatctgct cgaaatgaaa tgacgggttc ataccggata gttattctaa
    31021 tgactaataa acaagatcaa tcgatttatt tttcttatag tttttggaaa gaaagaaatg
    31081 agataggaag ttacggaata gttgatgaaa aaaagcaaaa agaaggaaat acgctcaata
    31141 aagttaaagt aacttactct aatggaaatg aggaaagtat ttaatgatta ctgacaaaca
    31201 atatagtgaa ttaagtgatg ctgtttactg gctagatcct aaacatagag actatgtacc
    31261 tgaaatgcaa gaaaatttaa gttttaaaat taacggcact atgtataaaa tcctaaaaat
    31321 caaaaactcc ttcgacggca tgcaggcgat ggctgtagcg ccaatcgtcc atagcaaatt
    31381 ggagaaaaat ttcaaaaata aaaaaattcc tgcaaacttt cgcgtactta aataatgaag
    31441 gtgtcaatac ttacataaaa agaaaggagg cacgttcttg tcaaggaaaa taagaaaact
    31501 cagcttagcc attttattaa tcgtcatatt agggatcgga gggaaagtct atatggacaa
    31561 aagagaagca caaaggcaga aagatttact cgcagtagaa aaacagtcag tcaaagttct
    31621 gaaaaatacc tttgcggata tcagggaagt gaaggtagaa gaatttaaaa aaaaccctgt
    31681 taccggaagt tatggtgcac ttgtgacaat gacaaataat gaagggaaat ctgtttattt
    31741 tgattatagt ttttggaagg agagaaatga acttggtggt tttggcattg agaacagagc
    31801 ggtacaaaag acaggagtca ctataaaaag agtaaaagta attttttcta acggacagga
    31861 ggagttagta tgacaataaa cgataagcat tacaatgata ttagtgagaa ggtttattgg
    31921 ctagatccca aatatccccg ctacaatgag ggatacaaaa agaactctgt taaagaattt
    31981 gctggtatgg aatttcaaat cctacaaatc aaagactcat tggacggcat gcaggcgatg
    32041 tctgtagcgc caatcaagaa cggtaaagtg gatctttctc aagtagtgat tgcttttgca
    32101 ggaacgaact taagtgattg gaaagatttg gaaacggatg caagaagtat catgaatctc
    32161 tacgggaata gaccaggagc aagtttttct gaaaaagcaa caacaaacaa gattttatcc
    32221 gttagtggat tgaaaacagc ggggcaaatc aattcagcga ttgcatttgc taatgaagta
    32281 aaaaaggaat atggagaagc tgctatttct actaccggcc attcattagg tggattctta
    32341 gcattatatg tagcagcaga gaaacaatgg aaaaatgtcg gcttcaacgg gccggacccg
    32401 tatgatctct tatcaccaga agcaaaaaaa tgggtggaag aaaatcccgg tatgctcacc
    32461 aattaccgaa atcacgccga ttttatcggt aattttggtg gtaatggcac aggtgctgaa
    32521 gttcggatca gtatggaaac agcattcttg aatctgcttg cttcccacag tttaacctct
    32581 tggaagtttg atgaaaatgg aaagctgatt attcctgaaa atgaaaacaa tgttaaagct
    32641 cgaaaacaac agaaagaaaa ccaagcgacc cagttgtttt cctatcagtt gcaagaactg
    32701 aacaacatac gtaaaaaact acgattaagt ggcggccagc tgacagttaa tgaacaaatt
    32761 tacttagatg acacacaagc taggttagta gtggaaaagt tatcggcaaa aatgaaaagc
    32821 gctatggaag aagttatccg catcaatcaa agaggaattt ttgagctaga gaaaaaatgg
    32881 caagaaggct tgcggcaagc acgtgttgta gccccttcgt tgactgaaca ggaaatcata
    32941 gaagcattag ctagtgtggg cgccacaaaa gaaaacttag tcgattctgc gaaagaagtc
    33001 tatctgctac gaatcaataa ggcaagacgt atgggggagg cctttgatac attggcgaag
    33061 gagatccaag acaaaatcaa tgaaatcgtg gcacgagatc gagaattagc acagcaattc
    33121 aattaaagga gaagtagatg aaactagaag aattggaaag agattatcga agtgaacgaa
    33181 gacaattaga agctcaagaa gatgaattga tccactttaa gaatcaagga ttacgattga
    33241 tggagcaaga ggagcagcga ctgcatcatg tattaaagaa cttagcttta gataatgagc
    33301 ctctgtttca agcaagacga agttattcga attatcagga gcattatcaa caagccctac
    33361 aagtagaacg acgaaaactc tatcagcagc aagatgaact agaacgttcc tatcgtcaaa
    33421 aaatcagaca aataactatg gagaaatcat cttatgaatg aatttttaat gcaattacta
    33481 aaagaattag agaaaaatct acatcgatca aatgtacgtt cagttggtat ggggatatct
    33541 cagcaaattt gggcagaaaa acgattgaca gatcaaaact accaaatgac tgcagaagaa
    33601 gctgctcaag aattttctaa ccgacatgta cacttgaaag gatcagtaaa agggcaattt
    33661 gcagaaaaac taactgaatt attccaaaga caagaaaaga aattaaaaca gtttcgttag
    33721 taaagttata tagaaaaatt ttgtgaaaat ggtt
//
LOCUS       DACEMK010000019        33706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEMK010000019 DACEMK010000000
VERSION     DACEMK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.019"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW715_000779"
     CDS             4..816
                     /locus_tag="IW715_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1021402.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW715_000780"
     CDS             813..1613
                     /locus_tag="IW715_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1021403.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW715_000781"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW715_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1021404.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW715_000782"
     CDS             3110..4819
                     /locus_tag="IW715_000782"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1021405.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IW715_000783"
     CDS             5031..9383
                     /locus_tag="IW715_000783"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1021406.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IW715_000784"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IW715_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1021407.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IW715_000785"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IW715_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1021408.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IW715_000786"
     CDS             11221..11514
                     /locus_tag="IW715_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1021409.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IW715_000787"
     CDS             11511..11822
                     /locus_tag="IW715_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1021410.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IW715_000788"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IW715_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1021411.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IW715_000789"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IW715_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1021412.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IW715_000790"
     CDS             complement(14623..14844)
                     /locus_tag="IW715_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1021413.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IW715_000791"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IW715_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IW715_000792"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IW715_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1021414.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IW715_000793"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IW715_000793"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1021415.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20242..21027
                     /locus_tag="IW715_000794"
     CDS             20242..21027
                     /locus_tag="IW715_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1021416.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21040..21834
                     /locus_tag="IW715_000795"
     CDS             21040..21834
                     /locus_tag="IW715_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1021417.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(22014..22466)
                     /locus_tag="IW715_000796"
     CDS             complement(22014..22466)
                     /locus_tag="IW715_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021418.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22931..23299)
                     /locus_tag="IW715_000797"
     CDS             complement(22931..23299)
                     /locus_tag="IW715_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1021419.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23449..23931
                     /locus_tag="IW715_000798"
     CDS             23449..23931
                     /locus_tag="IW715_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1021420.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23968..24978
                     /locus_tag="IW715_000799"
     CDS             23968..24978
                     /locus_tag="IW715_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1021421.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25093..25806)
                     /locus_tag="IW715_000800"
     CDS             complement(25093..25806)
                     /locus_tag="IW715_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021422.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25996..26586)
                     /locus_tag="IW715_000801"
     CDS             complement(25996..26586)
                     /locus_tag="IW715_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1021423.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27070..27297
                     /locus_tag="IW715_000802"
     CDS             27070..27297
                     /locus_tag="IW715_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1021424.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27313..28479
                     /locus_tag="IW715_000803"
     CDS             27313..28479
                     /locus_tag="IW715_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1021425.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28713..29756
                     /gene="hrcA"
                     /locus_tag="IW715_000804"
     CDS             28713..29756
                     /gene="hrcA"
                     /locus_tag="IW715_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1021426.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29850..30413
                     /gene="grpE"
                     /locus_tag="IW715_000805"
     CDS             29850..30413
                     /gene="grpE"
                     /locus_tag="IW715_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1021427.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30464..32293
                     /gene="dnaK"
                     /locus_tag="IW715_000806"
     CDS             30464..32293
                     /gene="dnaK"
                     /locus_tag="IW715_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1021428.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW715_000807"
     CDS             32444..33610
                     /gene="dnaJ"
                     /locus_tag="IW715_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1021429.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT        11454 a         5812 c         7275 g         9165 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc aggaaagggg ctgtgacaaa
    19981 agtcttgtca cagccttttt ttccaaataa atggtttttc agaagtagct tcttcggaaa
    20041 taagccgaaa tttcttgaag ctgaccactt ctgtcacaac ctctttcttt atagaaataa
    20101 aaggacacta gcaattcatg aaaacgattg ctagagcgta tcatttgctt tagactatat
    20161 atcttggtgt acactcactt taaggcggaa ataaaagtta tccgtaataa caggtttagg
    20221 tatgaaaaag gagtgggtac tatgtacaag gaattagaaa acaaagtcgc agtagtaact
    20281 ggtggctcaa aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat
    20341 gtagttgtca actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa
    20401 gaggcaggcg gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag
    20461 cgtttacttg atacggcttt agaaaatttt gggacactgg acgtttgggt taataatgct
    20521 gggatggaaa atcagcatcc aacccatgaa cttagccttg aagattggga aagagtgtta
    20581 aaagtcaatt tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag
    20641 aataataaaa aaggaaatat catcaatatg tcttctgttc atgaacagat cccatggcca
    20701 acgtttgctc attacgctgc ttcaaaaggc ggggtaaaat tatttaccga aacggtagca
    20761 atggagtatg caccgaaggg aatcagaatc aatagtatag gccctggtgc tatccgtaca
    20821 cctattaatg cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc
    20881 ccactaggac gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca
    20941 gacgaatcca gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat
    21001 ccttctttcc aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg
    21061 atcccggcag ttggctgggg gatccagcca ctgattttga aaaagattgg tggcagacca
    21121 acaaatgaga tattaggaac tggtattgga gcggtgcttg tcggcttact cgttcaatta
    21181 ttcgcttctc cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca
    21241 ttttgggtta ttggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa
    21301 acgatgccaa tttcgactgc attacagtta gtcggaacct cgttgattgg tgtcttcgct
    21361 tttggagaat ggtctggaag tatggggaaa atcattggag cagcagcaat cattcttctt
    21421 gtatttggtt ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca
    21481 aaaggaatta gtctattagc atcaacaagt attggttatt gggtatacag tgcattgcct
    21541 aaactagttg atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta
    21601 ggggcagtga tttatgtgat tttccgtaaa ccaaccgcat tttctgaaga caagagttgg
    21661 aaagcaacga tcgttggagc catctttagt gtgtcagcat tggcttatat tttttcagca
    21721 aatgaaaatg gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca
    21781 ttaggcggta tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa ttaacgatta
    21841 tcggattatt gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat
    21901 ggtaaaattt catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa
    21961 aaatagcgct agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat
    22021 acaagggaaa tatctgtctg gatcaataat ttttcattga tccattgact aaagtttacg
    22081 acataattga agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat
    22141 tgtaaaagac ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct
    22201 cggaattgat tgattgctgt aaaatcacga tattgaaggt tgaaacgcat cgtgattggt
    22261 gcatttagca tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg
    22321 aactcaaaaa gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt
    22381 tttccttcat gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat
    22441 tcatttactg agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact
    22501 tagttgatat ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta
    22561 aaaatcaagt cagaaaattc taacttttat ttctctaaag aaattcgttc tctcctaaag
    22621 tgatattata tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat
    22681 tttctcgtat tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt
    22741 actaagtaga aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc
    22801 ttataaaaat agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata
    22861 ggattgaaca aaacctgtat aaggtgatga atagtttcct cgccagtaaa aggtttattc
    22921 gtataaagct ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa
    22981 tcaattgttc aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat
    23041 ccgtatatac gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt
    23101 ccggtgcgat agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt
    23161 cagtaaacat aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta
    23221 tcttagcaaa aaggtctttg atcttttgtt gatgttcttt ttctttgatt tgattgatga
    23281 tcggctgaaa tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag
    23341 aaaaacaata gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat
    23401 ttatatgata agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg
    23461 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
    23521 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
    23581 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
    23641 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
    23701 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
    23761 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
    23821 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
    23881 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
    23941 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
    24001 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
    24061 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
    24121 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
    24181 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
    24241 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
    24301 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
    24361 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
    24421 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
    24481 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
    24541 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
    24601 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
    24661 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
    24721 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
    24781 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
    24841 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
    24901 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
    24961 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
    25021 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
    25081 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
    25141 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
    25201 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
    25261 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
    25321 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
    25381 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
    25441 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
    25501 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
    25561 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
    25621 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
    25681 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
    25741 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
    25801 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
    25861 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
    25921 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
    25981 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
    26041 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
    26101 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
    26161 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
    26221 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
    26281 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
    26341 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
    26401 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
    26461 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
    26521 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
    26581 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
    26641 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
    26701 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
    26761 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
    26821 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
    26881 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
    26941 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
    27001 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
    27061 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
    27121 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
    27181 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
    27241 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
    27301 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
    27361 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
    27421 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
    27481 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
    27541 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
    27601 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
    27661 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
    27721 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
    27781 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
    27841 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
    27901 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
    27961 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
    28021 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
    28081 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
    28141 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
    28201 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
    28261 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
    28321 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
    28381 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttttg
    28441 gggaacaatg tgtttgagcg ctttttgttt gaaaaatagt ttaaagaata tttgtttttt
    28501 atctgaattt aaataaaaaa agttcaaaaa tacaataaag gactattgaa acgttgatat
    28561 aactggattt acatcaatta tttagaatta gcactcttgt gtttagagtg ctaaaaaatg
    28621 gagtctttct cttgacagga gaagggcttc ttgctatatt tataagtgga ttagcactta
    28681 gaatatatga gtgctaatga gaggtgagga agatgttaac aaagcgacaa aacgacattt
    28741 tacgtctgat catccaacac tatacgagta gtggcgtgcc cgttggttcc aaaacattga
    28801 tgtctgaggg agtcaaggcc agctcagcaa cgattcgcaa tgatatgaaa gcattagaag
    28861 atgaaggatt gctgttgaag actcactctt cttctgggcg gattccatct gctttaggct
    28921 atcgttatta cgtggatcat ttgttaaaac ctgctcgcgt agcagaagat gatttgctgc
    28981 tgattcgtca gtctctaggc agagaatttc atgaaatcaa tgagattatt aagcaatctg
    29041 ctgagatatt atctgagctg acaagctata cgactttttc attgggacct gaaataaaag
    29101 accgccgact gactggcttt cgtatcgtac ccttgaataa tcgacagatt attgctatcg
    29161 tcgtaacaga tatggggaat gtagaaagcc aagtgttcac tcttcccgaa tatatgggaa
    29221 gtcaggaact agaaaaaatg gtacagattg taaacgaccg tctagtcgga gatcctctga
    29281 tgacggtgta ccataaactt cgtacggaga ttccgatgat tcttcataaa tatttccaaa
    29341 cgacagaagg gatgtctcag ttatttgaca ccgttcttgg tcatgttttt gaagataagg
    29401 tttatgtcag tggacaaatg aatctcttgg attatgagcc ccgtcaagat cttgatcagt
    29461 tcaaatcgat gttttctttt atgaaagact cagacggact tacacaaatg atcactccga
    29521 tggatagcaa tattcatatc aagattggtg ctgaattagg caatgattta ttgcagaata
    29581 tgagtatgat tcaagcaagt tacgagatag atggtcatgg aagaggtaca atagccttgt
    29641 tagggcctac tagtatgcct tattctcgga tgttcggtct agtcgatgtt tatcgaagag
    29701 aactagcagc taaacttgct gattactacc gttcgcttga cttgtcaaac tcgtaatgtg
    29761 aataacgaag ctatttgcag ctgatcgttc tctctttctt agtaagagca atcaaacagc
    29821 tgactgaata atgaaaggaa gactgtatcg tgaaagaaaa tcaagaagaa ctagataaag
    29881 aaatgacaga tgctcagcca gaaccggaaa tcgatgtcga agctgcagaa gattcgggta
    29941 tttcagaagc tgaagcggaa gaattcgaaa cagcgaaatt aaaagctgag ttagaagaaa
    30001 tggaagataa atacttgcgg gctcgtgctg aaatcgcaaa tatggcaaat cgaggaaaaa
    30061 atgagcgtga gcaattacaa aaataccgtt cacaagattt agccaaaaaa ttacttccat
    30121 caattgacaa cctggaacgt gcattagcca cagaagtgtc agatgatcaa ggggcaggtt
    30181 tgaaaaaagg cgtggaaatg gtcttggaaa gtttgcgtaa cgcactggaa gaagaaggaa
    30241 ttgaaaaaat ccctgctaaa ggagaagcct ttgatcctaa tcttcatcaa gcagtacaaa
    30301 ctgttccagc gactgaagat acaccagccg atacgattgt agaagtacta caagaaggct
    30361 acaaattaca tgatcgcgtg ttaagaccaa ccatggtcat tgttgcacaa taattgtgcg
    30421 gatataaaac ttacactttc aattaaagaa ggagacgtta attatgagta aaattatcgg
    30481 tattgactta ggtacgacta actctgcagt agcagtattg gaaggaaacg aagcaaaaat
    30541 catcgctaac ccagaaggaa accgtacaac accttctgtt gtatcattca aaaatggaga
    30601 aatccaagta ggggaagttg caaaacgtca agctgtgaca aacccaaata cgatttcatc
    30661 aatcaaacgc catatcggtg aagcaggtta caaagtagat gtagaaggta aatcatatac
    30721 accacaagaa atctcagcaa tgatccttca atatatcaaa ggattcgcag aagactattt
    30781 aggtgaaaaa gtcgaaaaag cagttatcac tgtgccagct tactttaatg atgcacaacg
    30841 tcaagcaaca aaagatgctg gtaagatcgc tggtctagaa gtagaacgta tcgtcaatga
    30901 accaactgca gctgctcttg cctatggttt agataaaaca gatcgcgacg aaaaaatctt
    30961 agtattcgac cttggtggcg gtacttttga cgtatctatc cttgaactag gagacggtgt
    31021 ctttgatgta ttgtcaacag ctggcgacaa ccatctaggt ggggacgact ttgataacaa
    31081 aatcatcgac tacatggtag cagaattcaa aaaagaaaac agcattgact tgtctcaaga
    31141 caaaatggct ttacaacgct tgaaagacgc tgctgaaaaa gcgaaaaaag atttatctgg
    31201 cgtatcaagc acacaaatca gcttgccatt tatcacagca ggagaatctg gaccattgca
    31261 tttggaaatg acattgacac gtgcaaaatt tgatgaatta acagctgact tagtagaacg
    31321 tacaaaagta ccagtacgtc aagcgctaaa agatgcaggt ttatcacaat cagaaatcga
    31381 cgaagttatt ttagtcggtg gatctactcg tatccctgca gttgtagaag cagtacgtaa
    31441 agaaacagga aaagaaccaa acaaatcagt taaccctgat gaagtagttg ctatgggtgc
    31501 tgctatccaa ggtggcgtga tcactggtga tgtgaaagat gttgttttac ttgacgttac
    31561 accattgtca ttaggtatcg aaacaatggg tggagtgttc actaaattga ttgaccgcaa
    31621 tacaacgatt ccaacaagta aatcacaagt attctcaact gcggcagata atcaaccagc
    31681 agtagacatc catgtattac aaggtgaacg cccaatggct gctgacaaca aaactctagg
    31741 aagattccaa ttgacagata ttccagcagc tcctcgtggt attccacaaa tcgaagtaac
    31801 attcgatatt gataaaaacg ggattgtaaa tgtatctgcg aaagatctag gtacacaaaa
    31861 agaacaaaaa atcacgatca aatcttcttc aggtctaaca gacgaagaaa tcgaacgtat
    31921 ggtaaaagat gcagaagcaa atgcagaagc agataaagct cgtaaagagg aagttgacct
    31981 acgtaatgat gtagatgcat tgctattctc tgtggacaaa acattgaaag aattagaagg
    32041 caaagtggac gaagaagaag tgaaaaaagc agaaacagct cgcgatgaat tgaaagccgc
    32101 tgttgaagca aacaacatcg aagaaatgaa aactaaacgc gatgcattga acgaaatcgt
    32161 tcaaaacttg actgttaaac tatacgaaca agcggcacaa caacaagctc aagaaaaccc
    32221 agaagcagct caaggcggag cagatgatgt cgttgatgct gattttgaag aagtcgatgg
    32281 cgacgataaa taatcagttc actgaatttc gagagaaggc caaagttgct actttggctt
    32341 tttcttttga aaaatagttt gaagtatcgg tttttgtttc aaacaaaata tgatatgata
    32401 acaagcgacg tgaaaatgat gagaaccaat aggaggaaag cctatggcaa caaaacgtga
    32461 ttattatgaa gtcttagggc tctcaaaagg agcatcagaa gatgaaataa agaaagctta
    32521 tcgtaagctt tcgaaaaaat accatcctga catcaataaa gaacctgatg cggaagaaaa
    32581 attcaaagaa gtatcagaag catatgagat tttaagtgac ccgcaaaaac gcgctgcata
    32641 tgaccaatac gggcatgcag gcacagatcc aaattacggt gctggtggag ctggtggcgg
    32701 atttggtgga ttcggcggct tctctggtgg cggcttcggt ggttttgaag atatcttcga
    32761 ctctttcttt ggaggaggcg gtcggtcagt cgatccaaat gcgcctcgtc aaggtgctga
    32821 tttgcaatat acggtcgact tgtcttttga agaagcgatt tttggtgtag aaaaagaaat
    32881 caagtataat cgggaagaaa tctgtcatac ttgtggtggg aacggtgcaa aaccaggcac
    32941 acaaccaact acttgtcata aatgtcatgg tgcaggtacg attaatgtag aacgccaaac
    33001 accacttgga cgagtgatga gccgtcaaac ttgtgatgtt tgtcatggga cagggaaaga
    33061 aatcaaagaa ccttgtccaa cctgtcatgg ctcaggacat gagaagaaag cacattcagt
    33121 taaagtgaat gtaccagctg gtgtagaaga aggacaacaa atgcgtttag ctggtcaagg
    33181 agaagctgga gaaaacggcg gaccatttgg tgatttgtat gtggtcttcc gtgtagaaga
    33241 aagtgatatc tttgatcgtg aaggttctga aatttactat gaattgcctt taagttttgt
    33301 acaagcagct ttgggggatg aagtccaagt accaactgtc catggagatg tgaaattgaa
    33361 gatccctgct ggaacacaga caggtacgaa cttccgttta cgtggaaaag gcgctccgaa
    33421 gttacgtggt ggtgcaacag gcgaccaaca tgtcaaagta aaactgatca caccgaaaaa
    33481 tctaaatgaa caacaaagag aagcattacg tgcattcgct gaagctggcg gcatcaaagt
    33541 tgaagaacag caagaagacg gtttcttcga taagtttaaa gatgcttttg gcgggaaaaa
    33601 gcggaagtag tttttctagt aaaaacgcta agtcattaat aattaaaaac cagacgaaca
    33661 atcatatgtt cgcctggttt ttttgtagtt gtcatgtttg taccaa
//
LOCUS       DACEMK010000020        31749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEMK010000020 DACEMK010000000
VERSION     DACEMK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.020"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(322..2214)
                     /locus_tag="IW715_000808"
     CDS             complement(322..2214)
                     /locus_tag="IW715_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021430.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2211..3938)
                     /locus_tag="IW715_000809"
     CDS             complement(2211..3938)
                     /locus_tag="IW715_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021431.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3954..4604)
                     /locus_tag="IW715_000810"
     CDS             complement(3954..4604)
                     /locus_tag="IW715_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1021432.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4808..5104
                     /locus_tag="IW715_000811"
     CDS             4808..5104
                     /locus_tag="IW715_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1021433.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5296..5916
                     /locus_tag="IW715_000812"
     CDS             5296..5916
                     /locus_tag="IW715_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1021434.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6008..7174)
                     /locus_tag="IW715_000813"
     CDS             complement(6008..7174)
                     /locus_tag="IW715_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1021435.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7186..7539)
                     /locus_tag="IW715_000814"
     CDS             complement(7186..7539)
                     /locus_tag="IW715_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1021436.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7667..8689
                     /locus_tag="IW715_000815"
     CDS             7667..8689
                     /locus_tag="IW715_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1021437.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9128..9562)
                     /locus_tag="IW715_000816"
     CDS             complement(9128..9562)
                     /locus_tag="IW715_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021438.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9672..11531)
                     /locus_tag="IW715_000817"
     CDS             complement(9672..11531)
                     /locus_tag="IW715_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1021439.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11649..12808)
                     /locus_tag="IW715_000818"
                     /pseudo
     CDS             complement(11649..12808)
                     /locus_tag="IW715_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13115..14539)
                     /gene="araA"
                     /locus_tag="IW715_000819"
     CDS             complement(13115..14539)
                     /gene="araA"
                     /locus_tag="IW715_000819"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1021440.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14555..15253)
                     /locus_tag="IW715_000820"
     CDS             complement(14555..15253)
                     /locus_tag="IW715_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1021441.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15210..16871)
                     /locus_tag="IW715_000821"
     CDS             complement(15210..16871)
                     /locus_tag="IW715_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1021442.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17089..18489
                     /locus_tag="IW715_000822"
     CDS             17089..18489
                     /locus_tag="IW715_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1021443.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18741..19820)
                     /locus_tag="IW715_000823"
     CDS             complement(18741..19820)
                     /locus_tag="IW715_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1021444.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19906..20865)
                     /locus_tag="IW715_000824"
     CDS             complement(19906..20865)
                     /locus_tag="IW715_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1021445.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20877..22400)
                     /locus_tag="IW715_000825"
     CDS             complement(20877..22400)
                     /locus_tag="IW715_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1021446.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22410..22973)
                     /locus_tag="IW715_000826"
     CDS             complement(22410..22973)
                     /locus_tag="IW715_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1021447.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22979..23842)
                     /locus_tag="IW715_000827"
     CDS             complement(22979..23842)
                     /locus_tag="IW715_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1021448.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23856..24752)
                     /locus_tag="IW715_000828"
     CDS             complement(23856..24752)
                     /locus_tag="IW715_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1021449.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24927..26198)
                     /locus_tag="IW715_000829"
     CDS             complement(24927..26198)
                     /locus_tag="IW715_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021450.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26188..27705)
                     /locus_tag="IW715_000830"
     CDS             complement(26188..27705)
                     /locus_tag="IW715_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1021451.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27903..28793
                     /locus_tag="IW715_000831"
     CDS             27903..28793
                     /locus_tag="IW715_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1021452.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28866..29690)
                     /gene="nadE"
                     /locus_tag="IW715_000832"
     CDS             complement(28866..29690)
                     /gene="nadE"
                     /locus_tag="IW715_000832"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1021453.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29702..31174)
                     /locus_tag="IW715_000833"
     CDS             complement(29702..31174)
                     /locus_tag="IW715_000833"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1021454.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9292 a         6658 c         5503 g        10296 t
ORIGIN      
        1 accgtttacc aagaggttgt gagcctgccg tttaactccg agtcacaagg agcaatttca
       61 aaaaacagct tctcaggttt gttgactatt gtgacttgtg accgaggagt tggcaggaga
      121 acaccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagatacaaa
      181 acggaaaaat agcttctcat attttgccgt tttttgagct aataccgaag gacagctgat
      241 tgaacaccgt ttattcggaa aaagggactg tgacacgact tttgtcacag cccctaaaaa
      301 aaataataaa tttgtactat tctatgaagc ttcttcttca gaaaactgac tgttataaag
      361 atctgcatag aatccgtctt tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga
      421 accatgattc atcacaataa tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac
      481 aacgaaactt gtccggtttt ccaataggcg gttcatcgct ttttgaatca gtatttctgt
      541 tcgtgtatct acactagatg ttgcttcatc aaggatcagg acatctggat ttgctaagaa
      601 tgcccgcgca attgtgatta gctgacgctg cccttgcgaa atattgcttg catcctcgtt
      661 taatacagtg ttatatcctt ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt
      721 agcagcctca atgatttgtt cgtctgttgc atcgtcgttc ccatatttga tattgtcata
      781 gatacttcct gtaaacagcc aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc
      841 atcacgtgaa agatcacggg tatctacacc atcgtaacga atactacctc cagaaacatc
      901 gtagaaccgt tcaagaaggt tgatcagtgt cgtcttccct gcaccagtcg gacctacaat
      961 agcgaccatc tcaccaggtt tgacatccaa gttaaaatca gtcattagca agttatctgg
     1021 tgtgtagccg aattgaacat tttcaaaacg aactttgtat ggtgtatctt tttcttctgg
     1081 aaggccagat ttttcattgg acatctcttc ttcatccaac acttcaaata cccgttcggc
     1141 tgaagcaact gttgattgga tagtgttcat caaacttgca agttgagtga ttggttgaga
     1201 gaactgtgtt gtatattgca agaacgcttg aacatcccct aatgtcatgt tcccatttgc
     1261 aacttttact ccgcctagta cagcgacgaa cacatagcct agatttttaa cgaaattcat
     1321 caacggcatg atgattgccg aaataaattg agctttccaa ccagcggcat acagattctg
     1381 attttctttt tcaaatactt cctggtcttt ttcagtatga ttgaatgttt taacaatggt
     1441 atgaccacca tatgtttctt ctacttggtt attcaataaa cctaaacttt tttgctgatc
     1501 tgcgaaaaac acttgtgatt tcggcgctac gatcatcacg atgatcaagc ttaaagggat
     1561 catcaataac gcgactaacg tcaattgcca gctgattgaa agcatcatca cgatgacccc
     1621 aatcaatgtg actgcactag ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat
     1681 gttatccata tcattgattg ctcgagacat gatatcccca tttgtatgtg tatcataata
     1741 ggaaatcggt acacgattca ttttggcttc caaatcatga cgtaattcat aaactgttcg
     1801 ttgggaaaca cgtgtcataa taaattgctg taaaaagttg aatacagcag aaatcaaata
     1861 catcacgatt actgtcgcaa tgatctgcgc gattttatca aagtctatcg gaaattttgt
     1921 tacttgttgg ccttgttgca tctgttgagc gccagccatt acacctttga atatttctgt
     1981 ggttgctttc cccaagacct ttggtgtttg gatttggaag atcacggctg ctattgctag
     2041 tacaaataca gcgatgatcg caaccatacg tttagacata tagccaaaca atcggcgtac
     2101 cgttccccag aaatttttag gcttttcacc ttttacaccg agatttctgc caggtccacc
     2161 atgtcctgga ccgcctgctg gtccatgaga gggacgtgtg tttgttttac tcatgctaag
     2221 tcctcctctc ttagttgtga atgaacgatt tcttgataag tttcgtttgt tgctaatagt
     2281 tcttcatgtg ttcctttgcc aaccagtttt ccttcatcta gcacaaggat catatctgca
     2341 tccattactg tacttactcg ttgagcaact aaaacggtga tactttcttt catattcgtt
     2401 ttcaatgctt ggcgaagatt ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca
     2461 aacacataga tatctgctgt tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt
     2521 ccaccagaga agtttcctcc tccttgttcc acatggctat ctaagccatc ttccatctct
     2581 gaaacaaaat ctttagcttg tgcgatttct aatgctttcc aaatcatttc atctgttgca
     2641 tccggcttac catactgcat attgtctcgg atcgttccag agaacagaac tgctttttga
     2701 ggaacaaagc ccattaattc tcgcaaatta tattgagtca tgtcactaat gtccgttcca
     2761 tttatttgga tcgaaccaga ttcgatgtca tataatcgag ggatcaaatt gactaaggtc
     2821 gttttacctg aaccagtccc ccctatgatt gcgacgatct cgcccgactt ggcttggaaa
     2881 tcaatatctt ccaatgcaag tttttctgct ccatgatatc gataattgac atgattgaag
     2941 gccaatgttg cgttttttcc agcaaaagaa actgttttag gattttcagg gtcatggata
     3001 ccgatttttt catccaacac ttcattaata cggtcagctg aagcttgtgc acgaggcacc
     3061 atgataaaaa tagcggaaag catcataaag ctgatcaaga tctgcatcgc ataagtcata
     3121 aatgcaatca gattaccgac ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa
     3181 gtgattgcta cgtttgtacc gctcatgatc aatgtcatca ttggcatcat caatgcgacg
     3241 atcgtattaa ctttgattgc cgtatttgta taatctttat tcgctaaatc aaaacgattt
     3301 tcttcaaagc gtgtcttatc gaatgctcga atcactctga cccctgtcaa tccttctcga
     3361 aataccaggt ttaaacggtc agttttcttt tgcatacttt tgaataatgg aacagcaaag
     3421 tacatgattc cgccgatcaa aataatcatt actgggatga caactaagaa gatttttgtc
     3481 aactgatgat ctttttgata agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg
     3541 atcatcatac gtaaaaacat ttgtgtaacc atttggattt gagtcacatc attggtcgtt
     3601 cttgtgatca aagaggccgt tccatattta tcaaacgcat cttttgtcag actctctgat
     3661 tttttataga catccgtacg caattgctta cccagtttct gtgattcacg agtagcaaaa
     3721 aatgtatttg cgatagcagc taagatactg atcaaagaaa agccgatcat cacaaaacct
     3781 gtatgccaaa tataatcgac atctccttgc gctacccctt tatcaataat attggaggta
     3841 agtgtcggca aatataaatc tgccagtact tggatcacca tgaaaactgc agctgcaatt
     3901 gctgaagtca aggagatccg ttttacgatt tttagcatct agttcccttc tttctattta
     3961 tatactccgt ttttcagcca agaaagtttc aaattgaatt cactgattgc ttgctctact
     4021 gagtaatctt cagatgttga aaataagcga tacgaatgcg caattgttgt aaatacagta
     4081 ttcatcacta attggatcag taattccaat tcatgatcat tttttacttt cagcttcgtc
     4141 cgatcgatca attcaagcaa ctcttttgca tcttgtttgt gttctttatg tggatcgtga
     4201 ggcgtttttt ggcgactcgt atgaggagcg acttttcttg aggcacggta atccatgttc
     4261 ataaacaagt tacgataaaa agaagcattc tcgccttcca aaatctcata aatcatttta
     4321 gaaaaatagg attcgaatcc tttgaataga tcaccatcgg attctttcat tagttgtaac
     4381 atatctttag agctgtcacg tctcattgtc tcaaagtaat aaaaataaag atcctcttta
     4441 tcttcaaaat actgatagaa actgcctcta gggatatccg ctaatttgac gatattagca
     4501 atcgaagctt cttttaaggg tactctagaa aactcgattc tcgcagcctc gattagtctt
     4561 ttttgttttt ctttaggcaa atggaaaaat gtctgtgtcg gcatatggat tctcctttct
     4621 ttcaagatga cacattgtca tatgacgcta tgtcattata acacctccta cctgttttgc
     4681 aagacatttg catagttttt ttcattatca tccgatacat taaaaaagtg ataagaggaa
     4741 tccttccatt tgaattaatg agagttgccc attaaaatag ccacatacta aacaaaggat
     4801 gagataaatg gcccaatcaa ttagtgtcca agacttcaat aaattgcctc tcgatgctga
     4861 cagcatcgtt ttagatacaa gaaatacctt agattacaca aaaaaccact taagccactc
     4921 gctttctatc cccgctgcta tcttgccaag acaattaaaa aatctagatc caaagaaact
     4981 ttattatatt ctaagctata caggcaggcg atcagaagca ttagccgatc aacttactgc
     5041 taatggtttt cgtgctgttt atatcattgg tgggatggaa gctcttcagt cgagtgttgc
     5101 ttgaatattg cttgttagat tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt
     5161 ttataccctg tcttttttta gttatttatt cttcaacctt atcaatatga cttgatagcc
     5221 ttttcttttt cgagtaaact gattttgtta gtcatagtta taacaatcat agcaataaaa
     5281 ggagaaggta agcatatggc atctgtttta gtagtcaaag gtcatcccct aactgcagaa
     5341 gaatcgcgca cagttaaggc attgacgtct tttttaacaa gttataaaga aaatcatcca
     5401 gatgatgaag tgactgtttt ggaactttat cgcgatgata ttccggaaat cgatgaagaa
     5461 ttattatcag gttgggaagc attacgtgct ggtgcagaat tcacttcctt atcagaaagt
     5521 caacaacaaa aaattgctcg tttcaatgaa ttgactgatc agttccttgc tgctgataaa
     5581 atcgttatcg caaacgcctt atggaatcta aatatcccaa caaaattaaa agcttggttc
     5641 gataccgtca atgtagctgg aaaaacgttc cgctatacag aaaacgggcc agaaggatta
     5701 gtgacaggta aaaaagcact gcacatccaa tctaatggcg gtgtatacaa cggacaagac
     5761 tttgcctctc aatatgtaaa aggtatttta aactttgtag gcatcgatca agtagatcag
     5821 ctattcatcg aaggaatcga ttatgaccct gaccgtgcag atgagttgat gcaaaatgca
     5881 ttagataaag ctgctgcttt aggtaaatca ttttaaaaat tgagataaat gcatacaaaa
     5941 gagacagagg aaaaaatctt caaagcagca tttgtgcctt ggatattttc ccatctgtct
     6001 ctttttgtta ttctttctgc tttttcaacg gattttcttc ataaagagaa gagattgttg
     6061 tgtaccattc aaaaacatga gtaatcacta ctttagctgc tgcataaaca ggaatcccca
     6121 agatcacacc gatgattccg aacaatttcc cagaagtcag cagaactaat aaaatcgtta
     6181 ctggatggat tgctaactga ttccctagta ctaatggaga gatcaagcgt ccttctattg
     6241 tctgctctac tgcaaaaacg atcagtactt tgatgatcat gaatggacct gctacaatgg
     6301 ctaaaaagat agccggaatc atcgctaaaa atgatcctaa gtaagggatc aggtttaaaa
     6361 aacccgcaat aatccccaac gtgacagcat actctaaacc aatgaccgca aaaccaatca
     6421 taaacatgat tgccacagca aaagccacag ttagttgtcc tcttatataa gaagaaactt
     6481 gactattcat ttcactcagc acattcaacg tcggtttttg catgcgtgtc ggtaaaaatt
     6541 tgacaaaata aggtgctaat tgtttaccgt ctttcaatag ataaaataag ataaacggca
     6601 tcgtcagcag agccactaaa atagtcgcta ccgcgccgac aaaacttcca agactctgaa
     6661 ctgtactctt agatatatct tggatcatat cgcctgccga attcatgaac ttatcacctg
     6721 ctgtttccaa ctgttcgcga aattggttga acagcgggtc ccgcaaaatc tcggttaatt
     6781 tattatcaat cgtatctaca tattgaggga agttactgat gaagctcact gtttgttcct
     6841 gaatcttagg gatgatcact acgctccccc atgcgattag tgcaacaacc aaaacaaaca
     6901 gcccaatgat actgtaaagc cttggtactt ttcgcttttc aaagtaatca actaccggat
     6961 tcatcaagta atacaagatt cctgctaaaa tgataggcag acctacgatt gctaaaaatt
     7021 gccagattgg tgcaaacaaa tatgaaactt ttgtaaataa aaagacgatc agcaatatta
     7081 acaatacaac aagtaaggct gtgactactt gattatttaa aaaccagcgc caaaaccacg
     7141 ataccggtct gttcttctta ttttttcctt ccataattcc tcctgttagt tataaaaaat
     7201 ttggcttccc aaaccaatag caggcagtct atgcggatga agatagattc catttgcaat
     7261 ttgtccttct tctggacaag catcaaaaat caatgatacg tgagctactg tctttaaatt
     7321 cccattggcc atcggaccga cataatcgat cgtatatacc tgatcatcaa aagcaattgt
     7381 tccgcctgct ttcaaagaaa aaggttcgtc ttttgatact tcttgaatca ccgaatattt
     7441 tcgtaaggca gctgttgctg ttttatcgaa caaaatcagc atgggttctt ccttgtttat
     7501 tgcttctgtc ccaatttctg taatctttgc ctgaatcatt gcactctctc ctttaattgc
     7561 tttcaaaaac agtataacac attctcattc tttagccgtg tggaataatt atgtgataga
     7621 ataaaaacga aaactatttc attacaatta ggaggaatca gctcatatga tcgaaaagat
     7681 aattttagga acctatacgc gtcgcgaaag taaaggaatc tatacgatcg accttgatac
     7741 agaaaaagaa gaattagcca acctttcatt agctgttgaa gaaaatagcc ctacctatgt
     7801 agcaaaaagt aaagctggca atctttacac agttactagt gtggatggat taggtggtgc
     7861 cggtgcgtac gataaggatt ttcatttttt gaatgcggta acagagtctg gcgctccttt
     7921 gtgttatgta gcggttgatg aagcacgtca attactatac ggggcgaact atcataaagg
     7981 tgaagtaaat gtctaccata tattaggaga tggcgaactg aaagcagcag attcaatatt
     8041 ccatcaagaa gcgacaggtc ctcataaaaa ccaagatcat gcgcatgtcc actatacgga
     8101 tctgactcct gaccaacgat tagtcgtatg tgatcttggt acagatcgtg tgtatactta
     8161 tgatgtgtcc gaaaacggca aattgaatct tgtaactacc ttcactgcag agccaggaac
     8221 aggccctcgc catcttgtct ttcatccaaa tggacaattc gcttatttat ttggtgaatt
     8281 agacagcaca gtccgcgtgc tttcttataa taaagaaaca ggtgcttttg aagaattaca
     8341 gaaaatatcg accttgccag aagaattcga cggtgaaaat ggaggggcag ctatccgcat
     8401 ttcaaatgat ggcaagttcc tttatgcttc taaccgagga cataactcga ttgctgtatt
     8461 cgcagttttt gaagatggtt cccacattca aaacaagcaa atcatctcaa cagaagggga
     8521 ttttccccgc gatttcgcac tagatccaag caataatttt gtcgtgtgtg ccaatcagaa
     8581 caccgataat ctgacattat atcgccgtca tcctgaaaca ggattactgg aattactgaa
     8641 aaaagatgtc tatgtaccag aatgtgtctg tgtttatttt gatcgttagt aagaaaagga
     8701 gaatgaaatg aatcagcaag acgtattaga cagactacgg gaagaactaa atattccttt
     8761 ctttgatggg aaaatcgaag ataaagagta tagcgaagag gaatatcaga aactcaaagc
     8821 cgatttacag aactactttg aacaatatgt acagaatgta gaaaactaat cagctttcca
     8881 tactatctga aaagaacatc aggacaagac ttttccgtct atcctgatgt tcttttctac
     8941 aaataaaaaa ccgccgagat ggttagactc gacggaaaag gagtatttac tcgattttga
     9001 gtaagtacaa ggttgttgtt ggttataggt atgtcattat cataaaggat tttgatcact
     9061 ctatcataaa ggtaatatta catttatatt agataactct agtttttatt gttatcaaag
     9121 atttctttta ttttttcaac aacaaattag tcattttttg gatggcagct gtcaattggt
     9181 ctgtcgcttg gtcttgtttt gcttcagatg tattgaatag atcttcatct acccctgatt
     9241 caagatcgat actttggatc atttcttcac agcctgctac atatgaaaga taagcttttt
     9301 caaaattttt atgaatgccc attgcttttg ctggtggacg caaagtactg actttcataa
     9361 gcattgcccg atattttttt gttccttctt caaataacag gatgatctct tcaagagtcg
     9421 atttgtccag ttcacttgtt tttccttcat caattgcgtt tctgatcact tcatatttcg
     9481 gatgcattcc ttctccgatt gcttctgttt ccttgacgat ttgatcgatg gactgcacat
     9541 aatagccaat attcggtaac atgttcgttt tcctactttc tccattgttg ttattattta
     9601 ttctacttgt ttttatcctc aaatgacaag tacttgaaaa aatttaatgg tttctataca
     9661 gctcgccatt cttattcgtg ctcaaagaaa ccatctgcta aactatattg tttccctgcg
     9721 acaatgtcat attggatcaa ttgatattcg tcgtaaatct cttgttgcga gcgattgaat
     9781 tgcgcttctt tataccactg tccgttttga tagtacagtt ctcgttcaaa cgccggaagt
     9841 tcctgttcta atgataaaag cagctgataa aagccatttg ttgtctgatt cgtctgttcc
     9901 attagattcg ctgcaaaata aaacggactg gtgattgcat cgtgagtcgt ttgcttttct
     9961 aattctcctt tactgtcgaa cataagaaat tgtgtttcat gcaatgtttg tttgtcattt
    10021 tttgcttgga tcgtatctga atagatccct ggaagatgat ccccccaaaa aacaactaaa
    10081 gtccttctag aaagattctt caattcttcc gtgaactgct tcagtgccgt gctgctatag
    10141 gaaatatctt gcagataatt ctctaagtca agtgtcccac ttccatctga agtttttgca
    10201 ggatagtcta gattatcata ctttccatcg tagggcatat gtgtctgcat cgtgactaaa
    10261 tgaatgaact gaggtgtttt ttcttccttg agaagtgtca ttacttcttt gtaagctgac
    10321 tcatcagaaa tatatggatt attctctatc gtatccgtat aggtcatcgt acgctcgctg
    10381 atgaactggt caaatcctaa tgtctgataa acatcctctc ttttatacat cgaagtattg
    10441 tatggatgga tcgctgtcgt ctggtatgat tgtgcgttta acgctgaaac gatagaagga
    10501 agctgatcca tttttggtac aagcatcgtg taaggggtgg tgagttgcgc attaaataac
    10561 gccatagaaa aaccagtcaa tgcttcaaat tcgatatttg ctgttccacc gccatagttt
    10621 tgagaaagca tattgccact atatgtttga tcagctactt cataataatc tgccaatggc
    10681 tcaccagata cttctacgcc gtttaatcga ctaggatctg agaaactttc actcatcacg
    10741 aacacgatgt tcggcgactc ttcctctaca gttttatttt tttcatcggc tagcttctgg
    10801 tatttttcag tgatttcttt gatcttttct ttcgaatatc cttcaggttc atccattggt
    10861 tctaccttca gattgtacaa aaacccaccg ataaaaccag tgttatagta gttcattttc
    10921 tggctgtaag gtatccatag tgccgtttta ttatatgctt ttcgcaacaa attatcggga
    10981 ttgttgaaat tactgatata ccctagcagt ccaattgcca caagcagtgt cgttaatcgg
    11041 atcaattgga atcgccttcc ttttttcagg cttcgaaaca tgtaccagca aaacaatcct
    11101 atcacggttc ctgcggcagc taagatcact gtaaacagca tagtgccggt catatctttc
    11161 agcaatccga tttcagtgat catcttcaaa tcgtctggat aaatcggttc ttgccgataa
    11221 ctcattttca tataatttgc aaaacctaat acaccgatgg ctacgacata cgtaagggaa
    11281 cctaaaggga cagatcctgc tagtgcaatc agaaaaataa gaataatcag caagacgaga
    11341 caggctaaga agaatttttc cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg
    11401 gatagatcat tttgacacca ctgcaaaaat aaattactaa aaacaacaag aaaaaagata
    11461 ctgatgattg ctgctgttcc ttttaaaatc tttttctgtt tggtcgaaag tctgcttata
    11521 tatcttttca aatgcttgct ccttcatttc tatcaataaa agaatgttgc ttctcttatt
    11581 ttagttgtac gaaattcgaa agtaaagaac ggaaagaaga attaatgaaa tccaaatatt
    11641 ctctttcttt atggcctttc tggtttcttt aataagcgga cctttcgtac tccagaaatt
    11701 ttttctagct gttgtttcac ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc
    11761 aatatgtaga catgctcatt cttatggtta cgcgatatat ctgcgatcgc tagctcgttc
    11821 tcattcagca gagaaaatat ctctgctaat atattggttt tcttttgata aaatatagtg
    11881 aatcgaaacg gcgcttggaa aaacattcgt gcggatggat aattgaccgc ttctctaacc
    11941 gtaccgaaca aaagataatc ttttaaggct tcaactgcca aacgtccgct gtctgccaaa
    12001 gcttcctgag tcgatcccct agatgaggca acataagtat acggtcattt tctaagaact
    12061 cttcttctgg aaaatcagtc aagtatttag ccagatgttg tttttccaat gcttgcataa
    12121 cagcatattt atcaacaatt tcacttcttc ctacattgat aagtacagca tcttttttca
    12181 ttttttctat attcgcctga tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga
    12241 ctacgatgta atccgaggta gaaagaactg tttctaaatc tgcttgccga acataatcca
    12301 actgggcatc gcggatagaa taacccaaga catccatccc taagctatag caacttagcg
    12361 ctacttttgt gcctatcgct ccaagaccaa gaagaccgat ggttttgcct tgcaattctc
    12421 gtcccacata agcacttctt ttgttttctg cctgttctaa aatattagga cctgttagtg
    12481 tttgtaccat acgacttgct tcgattatcg gacgggaaga taagagcaaa cagcacaaaa
    12541 caagctcttt tactgcatta gcatttacac ctggtgtatt catgacgatc gtcccgtttt
    12601 cagaagcttt ttcaacgttg atcgtgttca ccccaacacc agctcttgaa attgctagca
    12661 aatctggtgt aagccattca tctttcactt tcgtactacg aataactatt ccttcggctg
    12721 gcaaatcatc tattattttg aaatattccc cttgtaactt ttctatctct tgttctggaa
    12781 tagcatcaat caaatgaacg tgatacataa aaccccctcc ttactaacaa gtatctcgaa
    12841 atttctgcaa ttagacaact gtttaccatt tgacttccta gaagcaaaaa gagcagttag
    12901 agcagagtag gctgagtgat aaaaataagg ctatgacaag ttactgccat aagccttatt
    12961 ttcgagagca aaatattgag ggtgagatat cttgataaat ggtgttccgt tagctgacat
    13021 tgataataag actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt
    13081 tcttaaaact aagtggcttt ttcacaacct ctttttatct aataaccact gtttccaact
    13141 caactaattt tgcccacgtt tcaatttgat ctgttgttag atttagcgag acaacggtat
    13201 gatgaccgcc acctgcctga atccatgaat ggacaccttc atggaaattc ggtttagttt
    13261 tccataacac acgtgcaaca ggtaaattag gtgcttcagt cgttggttca aaggcttcta
    13321 cttcattgat cagcagttta taatgcgtac cgaaatctgc catcgatacg actacccctt
    13381 ctcctgcttt cccatcaaat acaagacgcg ccggatcttc tctatctccc attgataaag
    13441 gagaaacaac gattcttggt ttcgtcgcag caagcgtcgg atctacttcc atcatatggg
    13501 attccaagat tgcttcttgg ccactagcta gctcgtaagt ataatcttcc atgaatcctg
    13561 tatctttccc tcgagccatg attttcagca accgatcaat cgctgcagtt ttccagtcgc
    13621 cttccccagc aaaaccgtat ccttctgcca tcaaacgttg tacagctagt ccaggtagtt
    13681 gtttcattcc atacaggtct tcaaaattag tcgtaaaagc gttatagcct ccttcttcta
    13741 agaaacggcg aataccgatt tcctgctgag cttgtacttt tacatgttcc tcccatgtct
    13801 tttcttcata atcaccatag tcgaaggtat acaattcctt gtactcttca aataattgat
    13861 cgacttcttc gtctgatacg cgatcgatta cttgtacaag atctccgata ccaaaataat
    13921 caacagtcca accaaattga atctgtgctt ccactttgtc tccttctgtg acacccacgt
    13981 ttctcatgtt gtcaccaaaa cgagccactt ttattccaaa actttcatta taggctactg
    14041 caacatccat ccaatcagca atctcttttt ggatctcagg tcgtttccag tagccaacga
    14101 cgattttatt ttgtttattt aatcgagcat tgatgaaccc atattcgcgg tcaccatgag
    14161 cggattgatt taaattcatg aaatccatat cgatcgtttt ccaaggaatg ctttcgttat
    14221 attgcgtagc taaatgaagc aatggttttt gtagaagctt tgtcccgcgg atccacattt
    14281 ttgctggtga gaaagtatgc atccaagtga tcacacctgc tacttcttct cgataattga
    14341 cctctttcat gatttttgtg atcgtatcag cagtaactgc taattcttga aggcggattg
    14401 gataaggcaa ttggccattt tcattcagtt catcgaccat ttcttgtgca tgtttcttta
    14461 cttctcgcag tgcttcttcc ccatataaat gctgtgatcc tacaacaaac caaaattctt
    14521 tttctccaat attcaacata attagccact ccttttattt gttttgtccg taatacgcat
    14581 tttttccgtg ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa
    14641 atgaattgag ctgtctggta aataaattca tcttgcatac ttcatctagt acgactgcgt
    14701 tcattaccgc actttgcgca tctttccccc aagtaaatgg accgtgtccg tgtaaaagta
    14761 cccctggaat tgctaaggga tcaattttgc gttctttgaa tgtttctatg ataacttttc
    14821 ctgtttcata ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta
    14881 ccgatccata aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt
    14941 gtgcccagat cgtcgcccat gtcgagtgag tatggacgat tcctccgatt tgaggaaatg
    15001 aacgatataa aacagcatga gtaggcgtat ccgaagaggg attgagttta ccttcaatga
    15061 tatgattgtc caagtccaca acgaccatgt cgtctgctgt catctcttca taactgactc
    15121 cgctaggttt gataacgaaa tatcgtgttt caggatcaaa agcgctcaca tttccccatg
    15181 tatatttgac gagtccgtgt ttaggcaagt caagatttgc ttggtatact tcttctttca
    15241 gttgttccag cattttctat cactcctttt tcttgcaatt gatgaatcgc tgttttttca
    15301 atgtcgatcc cctcacggta acgtctcata aatatctgat agcctgctat ttcttctgct
    15361 gaaggtgtga tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa gaagttttct
    15421 aaagacttcc catgtcgatc catcatatag gctgctaata acgcgattcc ccacgctcca
    15481 ccttctccgg ctgtgtccat cactgtaact ggtgcctcca tagcagatgc tagaatccgc
    15541 tgtccaactt ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc aatttggaca
    15601 ttctcagatt tcaatatatc cattccgatt ctcattgcac caaaagcact ggctaaatgg
    15661 atccgcatga aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa taatggcctt
    15721 ccttcattta ctcctgtaat gttttcaccg gaaaaataac cataagaaag taatcccccg
    15781 caatccgtat cgccttctag cgctttgttg aataataccg tgaataattt atctctgctt
    15841 atctccatac ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc attgatatcc
    15901 gaagagcagt tattcgtatg aaccatgcct actagttctc ctgaaggtgt agtgaccatg
    15961 tcgatttccg gatggacatt ttctagttct ttttctaata cgatcatagc gaaggcggaa
    16021 gtaccagccg aaatgttccc tgtccctttg gctacactgt tcgtagcgac cataccggtt
    16081 cctgcatccc cttctggcgg acaaaccggg attcctggtc tcaaatttcc cgtagggtcg
    16141 atcagataag cccctgattc gcttaacttg ccagcttctt ctcctgctac tagaacttta
    16201 ggcaaaatgt gatccaagtc aatcgctacg tgcgcttttt cgattaactg accaaaaata
    16261 tttttcattt cttgatggta ggttttcgtt gaaggatcga taggaaacat gcctgatgca
    16321 tctcctacgc ctagtacttt ctcaccagta agctgccaat ggacataacc tgcaagcgtt
    16381 gtcaaaaaat taatttcttt cacatgttct tctttattca aaatagattg atacaaatga
    16441 gcgatactcc atctctgcgg aatattgtat tgaaattttt ccgttaatat tcgttctgct
    16501 tcttcagtga ttgtatttcg ccaagtacga aatggtacta gtaactgttc cttgctatca
    16561 aatgctagat aaccgtgcat cattgcactg aacccaatcg acccgattgt cgtaagattc
    16621 tcgccataga tttcgaatac ttctgctgcc atttttcggt aactgacgcg cagtcctttc
    16681 caaatatctt ctaatgaata ggtccagata ccattttcta gtttgttttc ccactcataa
    16741 ctaccagcgg caataggagt aaaagatgaa tcaatcaata cagctttgat cctagttgat
    16801 ccaaactcaa tccctaaaga tgtacgacca tctatgacat ctaacatact ttgcgcattt
    16861 acttccttca taatgattta actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt
    16921 agtagcgctt acaatgatta ttgtatactt ttgtacgtac atgtcaagca gttatttaca
    16981 agaatacaat aaaaaacact tgttttgtac gtacataagt tataaaatga aattgcttac
    17041 aaaatctgat caaccgcctt ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa
    17101 aaaaaagttt cctcatcgtt tatttatttt tttggcgcat ttggcggcat tctatttgga
    17161 tatgatattg gtgttatgac cggcgcctta ccttttttac agcatgattg gggtttggca
    17221 ggtaaagctt ctttgatagg atggatcacg tcttcggtca tgttaggtgc gatccttggc
    17281 ggctctttat ctggtcttct ttctgataag cttggacgac gaaaaatgat tcttctttct
    17341 gcacttattt ttatggctgg ttctgtcctt tctgcatctg cccctcacaa tggttcttat
    17401 tttctgattg cagcaaggat tcttctaggt ttagctgttg gcgctgcatc tgccctagtt
    17461 cctgcttata tgtctgaaat ggctcctgct agactaaggg gaagattgtc agggatcaat
    17521 caagtgatga ttgcttctgg catgctcctt tcctatgttg cagattattt gctaaaaggg
    17581 ttgcctgaaa cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt
    17641 ttatttttcg gtgttttagc tctaccagag tcaccgcgtt ttcttatgca atctggccgc
    17701 ttggaagagg caaaaagagt attgaactat attcggacac caaaagaagc tgaacaggaa
    17761 ttcgaacaaa tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact
    17821 ttgtttttag aaaaatatcg ttccttagtc attgccggta ttggtgtagc agtattccag
    17881 caatttcaag gagctaatgc tattttttac tatattccgc tgatcgtaga aaaagcgact
    17941 gggcatgcag cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat tttagttgct
    18001 ggttctttgc tctttttggt tattgcagac aagttcaatc ggcgaacttt attgaaaata
    18061 ggcggtagcg tgatgggact ttctttcatc ctaccagctg ttttaggaac tgtactggat
    18121 gctcatacaa attcgttgtt gattctcttg tttttatgta tttatgtcgc attttattct
    18181 tgcacatggg ctccactgac atgggtaatc gtaggcgaaa tattcccact agctgtccgc
    18241 gggcgagcat ctggcctagc ctcttctttt aattggattg gttctttcct agttggcttg
    18301 ctttttcctg tgatgactgc ttctatgtct caagaaatcg ttttcggcat ttttgggatc
    18361 atctgctttt taggggtttt atttattcaa gagatcgtac cagaaactcg tgggaaatct
    18421 ttagaggaaa tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat ttctatccat
    18481 cattcttaaa gaggttgtga cagaagtggt cagcttcaag aaataagaag gaaccgctta
    18541 gtttccagtc acaaggaaca atttcaaaaa acagcttctc atgtttgatg aatattgtga
    18601 cttgtgacca gttggtagga gaacaccgtt tagtggagag gtggtgaaag aaacgttcag
    18661 ccttgagcga tgacaaaagc tctcacaaac attgcgacga gtaaccgtag gagcaataag
    18721 aggcggtaaa aacccgtctc ttatttttcg ttttctttca ctgtttcccg ttcaactagt
    18781 acaggttcat agagaatatt tggctgtgcc tgtccttttt caatcgttgt gacgatccac
    18841 cgtgcggcat cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa ttttttattc
    18901 cctaaatggc ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc tggaatatga
    18961 tagccatttt ctgtcaactt atctagcatt aaactagcta tttgatcgtt atagcaaatg
    19021 atgccatcta aagcttgttc ttctttcaac cgttgtactg ttttttcagc gattgtttct
    19081 cgtgtttccg tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt atactgttca
    19141 cacgcgctga taaaaccttt catcctgtat tttccttgaa gatcgtcgat ttttgtgatg
    19201 aacaacagac gctgatgtcc tttttttatc aaatattctg ctgccataaa accgactttc
    19261 acatcatcta aacaaatata agaggcattt aactcttcat aagcagcatt gatcatcacc
    19321 atcggaatgc cttgttctct caaagcaaca taaagtgcta agtttgggtt atactgatta
    19381 cttttcgtag gctcaacgat caaaccatct accccataag caatcatttt cttcaaacac
    19441 tctttctcca attcgtgatc gttattcgta ctggctaaaa gcaatgaata cccagattct
    19501 cgtaattcat tttcgattcc tcggataatc gacggaaaaa tataatcaga gagatatgtg
    19561 gtgatgacac cgatcgtttt tgttctgttc aatggtgttt tttctttctt atagctgtca
    19621 tctacatatg tgcccgaccc tttttcttta cgcagatagc cctcatttac caacacagcg
    19681 attgcttgcc gaatcgtatg tctgctgacg tcatatattt cttgcagctt gaattctgag
    19741 ggaatggttg tatttacttc gtattcctca cgaaggatct tttgttttat ctcttctgca
    19801 atatcttgat attttgccat ttgccttgcc cctttttatt tgtacgtaca tatatcataa
    19861 cgagtctgca aggtttattc aagcaaaaat caaggtttct tgtgctcata aggcatacgg
    19921 aacaggttca ccaaattctg gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc
    19981 gtgccgatta ggattgtcta acggatcccc tttttggttc ttctctggtc gagcatgata
    20041 gatcaaaata tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac caggaccaaa
    20101 ttgcttattt ttttcagaag aaacgaatac tggctccgat aatttatgcc atgattcttt
    20161 taataaaaga ttcttgtgct catcagccca cagcaggccc atggcatagt tttcatctgt
    20221 tgcactacca gaatacgtaa tgaatatttt cccattgcga gtaaggactg ctggaccttc
    20281 atttactgaa aaaccccgtt tttcccattc atactcaggt atagaaagga ggatctgctt
    20341 cccctttaac gtccaaggat tatccatttc ggatagataa agattagaat tcccagtaat
    20401 ttcaggatct tgttgggccc acacataata tagctttcct tgagaagaga aagttgttcc
    20461 gtctaaactg aaagtttccc ttttcgtttt tatttgtccc ttttctatcc aatctgtatt
    20521 cattggatct atatgctgat tttcaagtac gaacatgcgg tgatgatgag attcatcacg
    20581 tatagtttta tgattgcttg cagcaaagta aatataccat tttccattga tataatggac
    20641 ttcaggtgcc cagatcaatt cactcatcgg tccttcttca tgagcctgcc atacaatcag
    20701 gcttgcagca tgttctaatt cattcagtga ctttgccctt cttaattcaa tcacttggta
    20761 gcctggaacc gatcctgtaa aataataata accatctgta tgtttatata cccaaggatc
    20821 tgcccgttgc cgaacgatag gattattata aagtgtcttc atcataacaa atttcctcac
    20881 attttcttta ttctaattac attccaagag tatttttcta tccctacatt taattcgtca
    20941 tgtttttcta ttgaaaaata gtttatttca ggttttattt tttctggttc ttcacgtgta
    21001 tttttgtcca acagatcgct actggttaat gtgtagtgct ctccttgatc tagcaaattg
    21061 aaatcatgtt caatcgttat cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc
    21121 agctgattgc cgttatctac gatcacactg tccacataag gaacattttt tatcttttca
    21181 caatcataaa cagggcaaat gatttgttgt ttcaatactt gacctttagc atatttcgat
    21241 aagtgttgca tcacataata agttggattc ctccatgctt gtcctttagc atctgtcagt
    21301 attaaaggta ttgtgtttac taacaatgat tgacaactaa tctttacgcg atctgcgtgt
    21361 cgtaaaatag ccagccctaa tgaaccaaat aataatgtat cttccatagt aaagtaacac
    21421 caatcgatgg gactaccgac ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca
    21481 ttccattcat caaacgctag atacattgtt ttctcacttc tcaaatatcc ttttacataa
    21541 tcacaagtag caacgatttc ttcaatttga cgatcgaatc gataagcttt tgcaaggtat
    21601 gtcgctgtat catcacgctc atttttagga ggtttagtca agtcttcttc atcatactta
    21661 tcaatatagt tatgcaaagc taaataatct acattctcat aagcttcttc taatgcgata
    21721 cgatcccatt cagggtacga tggtaaacgt ggcgtagagc ttccaactaa gattgtttca
    21781 attgaatcat ccactaactt catgactttt gaggcttcat ttgctaacct accatattca
    21841 taagcactct tagcccccac ttgccatgga ccgtctagct cattccctaa acaccataat
    21901 ttaacgccaa aaggcttttt ttgcccattt tttattctta aatcactcca gaaggttcct
    21961 tttggaaaat tacaatattc tactaaattt tttgcttcat caattccttt tgttcctaaa
    22021 ttcaccgtga atatcggttt cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt
    22081 ccaaaagaat ttggttctat acctcgccat gctaaatcaa ttttttttgg tcgttgttct
    22141 ttcctaccta cagtatccac ccaatcataa ccagatgtaa agtttcctcc aggatagcga
    22201 atgacaccta attctaattc ctttacataa gagaggacat cttgtctaaa cccgtcacca
    22261 tcagctgttt catggcccgg ttcataaatc cctgtataga cgacacttcc catatgctca
    22321 acaaatgagc caaacagccg agaactgatc tctcctaccg taaaatcctc tctgattttt
    22381 actttcactt cttcgtccaa cagctttccc tacctttcat ttcctatagt caagataata
    22441 gatacaagtt tccatacgcc accagcaaag ataaaaataa aaggagggaa aagaaatatg
    22501 agcagtacaa atcctaataa caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat
    22561 actttccatg cactttgttc aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc
    22621 acatatatcc aggataaaag aaacatggaa taaataagta aaactacata tccactaatt
    22681 gaaagtacga ttgtctgttc ttctaataat tgattaacaa gaaaataaat aatgatcgtt
    22741 aacagactaa ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa
    22801 atgatatttc ctgatccctc acctttccat tgcgccatta cataaaaaac agcacatgta
    22861 gcaggcacga tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg
    22921 taatcagaaa ccacaatcaa ctgtccataa agtttagtct caactatttt catcttcctc
    22981 actctttcat tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa
    23041 ccactagtaa tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa
    23101 tgttctgccc tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg
    23161 tcaatatcaa tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg
    23221 ataaaagagt aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta
    23281 atccatcaat gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca
    23341 tcatgaagat accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa
    23401 gcatgccaag caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca
    23461 tcattgtcag caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa
    23521 atgcataccc cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa
    23581 tcactgtatt tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat
    23641 ctagggtata gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt
    23701 taaaagaaga aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca
    23761 tactaatcgt tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg
    23821 taatcgagct ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt
    23881 gacatgtaca cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac
    23941 agtgctgatg catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga
    24001 acagctgttt ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac
    24061 acttgcatcg aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga
    24121 ggtagcgtaa tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca
    24181 tacaaagagg gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa
    24241 ttcttccaaa cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat
    24301 tgagggatcg gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca
    24361 agcaacatag aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat
    24421 gtcgaacgac aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa
    24481 ccaacgataa tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa
    24541 ttaactgctc gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt
    24601 gatgtaaaga aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg
    24661 acagtaaata ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca
    24721 ggacgattcc aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata
    24781 aaatatacaa tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt
    24841 ttgcaataac acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa
    24901 cagtctatcc tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct
    24961 gcatcgttca tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg
    25021 atgatatcgt tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag
    25081 tccatttgtt tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct
    25141 actgaatcca agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt
    25201 gtattccaat atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc
    25261 ggaatggcat atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt
    25321 ggtaaggtag tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc
    25381 ccattgattt caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa
    25441 atcgattctg gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta
    25501 aggttctcag gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca
    25561 tttgctaaca tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat
    25621 acatttttac tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta
    25681 ggtggttgtt cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac
    25741 atgccttgta cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata
    25801 gccgtttctt taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt
    25861 tgtaccgctc cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca
    25921 ggtgcttttt tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg
    25981 atatccattt caattttgat gtttttatca tttgttttat taaattcatt aacgatttct
    26041 tgcaatattt caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta
    26101 tcagaatcag attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct
    26161 accaacaaag agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga
    26221 taaacgacat ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc
    26281 ttcagctttt acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt
    26341 tgcttttgta tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc
    26401 agacaagtga acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag
    26461 ttctttgtca tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt
    26521 cgcatagttt gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc
    26581 ttgcataaat ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat
    26641 tggtgcaata acatttacca attgagcaag acaagcaatc tttactctat ctgcatgttt
    26701 caataaagtg attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc
    26761 tagtaaagga ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata
    26821 ccaaatattc cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt
    26881 gactgtatcg cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc
    26941 aagataatga gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc
    27001 tatttgatcg tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg
    27061 ccgattcgaa cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt
    27121 agccgtttcc tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg
    27181 accgtccatt tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt
    27241 cgaacgcctt aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt
    27301 acgagctgca tctactccac gagtacccag attgacagcc atattgattt cggcccccac
    27361 atgctgacac cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca
    27421 agctaaatcc aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa
    27481 tcctgacaca aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa
    27541 gtgaatgacg tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat
    27601 cccttcatat actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac
    27661 tttgcctaca cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta
    27721 atttatacat gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt
    27781 acttattttt tatcgatttt aattaaccaa aatattaata atgattaaaa caaataattt
    27841 aatataactt ttattaacaa atatgttaat atataagaaa aaccttttaa gaggtgatat
    27901 atatgcaact acaaataaac gaagaatcct taccggtata tgaagcatta gctagtaaaa
    27961 caaggatcaa gatcatccag ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa
    28021 aagaacttgg agtgagcagt gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa
    28081 acatcatcaa aacagaaaaa gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa
    28141 tcgatatcag ttttccagaa aaaatattca atgcttttga tacgaaagaa acatcgatac
    28201 ctattggtca ctatacaaat tatgcaattg aaccaacatg cggtttagct acgatccatg
    28261 attttatcgg gaaagtggat gaaccacgct attttatgga tcctcgaagg atggatgccc
    28321 gcattctctg gtttaccagc ggatttgtcg agtatcaggc acctaatttt ttaaatacag
    28381 aggatacatt ggagatgttg gaagtttctg tggaaatttc ttctgaattt cctttttcaa
    28441 atgacaattg gccttcagat atcactttca gcctaaatgg tgtggagcta ggaacatgga
    28501 cgagccctgg agattttgct gatatccgcg gtaaatatac gcctgattgg tatccagata
    28561 atcttaatca atacggcttg ctgaagacga tccgaattac caaacacttg acgaatatga
    28621 atggagaacc cttgtcaaac atcacgatca acgacatccc taaagagcaa gacacttggc
    28681 atttgcgaat cgaagtgaaa gacgatgcca aacatgtggg gggctgtact ttatttggta
    28741 aaggattcgg taattacgat caggatatca aattaaaagt ttactacagc tgatattttt
    28801 cttttctatt ttataaaaca aaaacagcca atccctatga agggacggct gtttttgctt
    28861 tattgttatt tccagaaatc atccaaaacg gaaattggca aatgtcgttt atgttgtgtc
    28921 ttgttccacc atgcttcgat tttttcttgt gcttgagcgg gaacttcttt tccttctaga
    28981 taatcatcaa tttcattata tgtgacacct agtgcgactt catctgcaat cattggtttc
    29041 ccatcttcaa gatctgcagt cggaattttt gtgtataatt tttccggtgc attcaatgct
    29101 tgaagcaatt gtttcccttg tcgtttgtcc aaacggaata atggcaaaat gtcggctcca
    29161 ccatcaccaa atttcgtgaa gaatccagta atgttttctg cagcatgatc tgtccctaat
    29221 acggctcctg ctctttctcc agctacggca tattgcgtaa tcatacgttg acgtgctttg
    29281 atatttcctt tgttaaaatc gctgattccc acacctgctt cgcttaatac ttgtacttgt
    29341 gcatcaacag ctggtttgat atttactcgc agactaacat cgggctggat aaagtcaaga
    29401 gcagcttttg catcttcttc atctgcttgt tcaccataag gaagacgaac agcgataaat
    29461 tgatagtttt catcttttgt ttcttcacgc atttcttcca tcgttagttg cgccaaacgg
    29521 cctgctaaag tagaatcttg gccgccgctg atacctagta caaaagtttt aagaaacgga
    29581 tattttttca aatatgcttt cataaagtca atacttttac gtatttcttc ttttggatca
    29641 attgttggtt tgacacctaa ttcattgata atttctgtct gcaggctcat ttattctcct
    29701 cctatgcttc tgtatgaagt tctgctacgc tttttttcat tcgttccatg atagccattt
    29761 tatggttcca gcattcagta gacagatcga ctgggtattt ttgcggattc aagtctcgtt
    29821 tatactcttc ccatagtgaa tctagatttt cttttgtata ttctttgatt tcgtttagtg
    29881 caggtaattc gtaaactctt tttccttcga taaagatatc ttgtaaaact gggcgagctg
    29941 taaagtctcg aacagtttta ttgataaacg tatgcactgg atggaacatg aagatagctt
    30001 cttcttctcg agggtcttca tcccataatg tcacgtagtc tccttcagat ttcccatcaa
    30061 aatttcgagt gatgcgccac acttgttttt tccctggagt agttactttt tctgcattgc
    30121 ttgataactt gatcgtatcc atcatttttc cttcatcatc ttcgatcgat actagcttga
    30181 atactgcacc aagggctggc tgatcatagg ctgtgatcaa ttttgtccca acaccccata
    30241 catcaatttt agctttttgc atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg
    30301 aagcatagat ttttgcttcc gtaaatccgg ctgcatctaa ttgttcccga acacgcttag
    30361 atatataggc catatctcca ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta
    30421 attctttcgc cacgcgaatc gcacttggca cacctgattt caacgtatca taagtgtcga
    30481 ctaagaacac gcagtcttta tgggttttag cgtaggccat aaatgcttca taatcatttc
    30541 cataagattg gactaacgaa tgagcatggg tcccgctagc tggtatccca aatatcttcc
    30601 cagcacgtac attgcttgtt gcatctgctc cgccgatata tgccgctcta gttccccaga
    30661 ctgctgcatc cagttcttgt gcccgtctag ttccaaattc caatagtgga tcttctccga
    30721 tgacagattt gatacgagct gcttttgtcg cgatcaacgt ttggaaattg accatattca
    30781 acagtgctgt ttctactaat tggcagtgcg ctaaaggccc ttccacttgg attaatggtt
    30841 catttgcaaa aactagttct ccttcacgag cagaacgaac tgttgcttta aattcaaaat
    30901 tttgtaaata ctccaaaaaa ccttcaggat agacttctaa tccacgcaag taagcaatat
    30961 cggaatcatt gaacgtcaga ttctctaaat aattgactaa ccgttccaat cctgcaaaaa
    31021 ccgcgtatcc gtggttaaaa ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat
    31081 cagctcgacc taattcccag tatgtctgca tcatattgat ttgatataga tccgtatgca
    31141 gcgtcaaact gtcgtctggg taagtattat tcatcttgta ttactcctct tggcaagttt
    31201 tcatttccta cccattataa caaaaaaaat taataggaaa agaaatcttc cattttttct
    31261 gaaaaagcac atgttttacg gatatttctt gctaaaaatg aactccatct gctaaaacta
    31321 tcctttctta ttggtattta acctaaacga gaaagaatac ttattttatt caatgtatcc
    31381 gagacacgaa gaaattagcg ttttctattt ttcttacttg ataaaataag tagattttcc
    31441 tactacaatc gtagccgtta agataccgat gattgaacaa atataaccaa atgtctctac
    31501 aacataatca atggcaaaag tttttgaagc aataatacgt ctttcctttt ttgcttctta
    31561 tccccaaaga aacggatata acttattatg tattgaattt ttttggaaag gcgaattatg
    31621 acaagatata ccttgcgttg aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga
    31681 tacaaagtaa gaaagcagct gtcaatagaa tggaaacaaa aatggcgaga catcaatcag
    31741 tctcgcctt
//
LOCUS       DACEMK010000021        31747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEMK010000021 DACEMK010000000
VERSION     DACEMK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.021"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            360..728
                     /locus_tag="IW715_000834"
     CDS             360..728
                     /locus_tag="IW715_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1021455.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            908..1849
                     /locus_tag="IW715_000835"
     CDS             908..1849
                     /locus_tag="IW715_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1021456.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1918..2649
                     /locus_tag="IW715_000836"
     CDS             1918..2649
                     /locus_tag="IW715_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1021457.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2793..4973
                     /locus_tag="IW715_000837"
     CDS             2793..4973
                     /locus_tag="IW715_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1021458.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4993..5475
                     /locus_tag="IW715_000838"
     CDS             4993..5475
                     /locus_tag="IW715_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1021459.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5491..6252
                     /locus_tag="IW715_000839"
     CDS             5491..6252
                     /locus_tag="IW715_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1021460.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6383..7717
                     /locus_tag="IW715_000840"
     CDS             6383..7717
                     /locus_tag="IW715_000840"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1021461.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8024..8509
                     /locus_tag="IW715_000841"
     CDS             8024..8509
                     /locus_tag="IW715_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ1021462.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8502..9185
                     /locus_tag="IW715_000842"
     CDS             8502..9185
                     /locus_tag="IW715_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1021463.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9311..11956
                     /gene="polA"
                     /locus_tag="IW715_000843"
     CDS             9311..11956
                     /gene="polA"
                     /locus_tag="IW715_000843"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1021464.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12001..12837
                     /gene="mutM"
                     /locus_tag="IW715_000844"
     CDS             12001..12837
                     /gene="mutM"
                     /locus_tag="IW715_000844"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1021465.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12801..13430
                     /locus_tag="IW715_000845"
     CDS             12801..13430
                     /locus_tag="IW715_000845"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1021466.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13482..13814
                     /locus_tag="IW715_000846"
     CDS             13482..13814
                     /locus_tag="IW715_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1021467.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14126..14617
                     /gene="nrdR"
                     /locus_tag="IW715_000847"
     CDS             14126..14617
                     /gene="nrdR"
                     /locus_tag="IW715_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1021468.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14640..16013
                     /locus_tag="IW715_000848"
     CDS             14640..16013
                     /locus_tag="IW715_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1021469.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16013..16939
                     /gene="dnaI"
                     /locus_tag="IW715_000849"
     CDS             16013..16939
                     /gene="dnaI"
                     /locus_tag="IW715_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1021470.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17068..17508
                     /locus_tag="IW715_000850"
     CDS             17068..17508
                     /locus_tag="IW715_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1021471.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17652..18353
                     /locus_tag="IW715_000851"
     CDS             17652..18353
                     /locus_tag="IW715_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1021472.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18350..19471
                     /locus_tag="IW715_000852"
     CDS             18350..19471
                     /locus_tag="IW715_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1021473.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19486..20718
                     /gene="pepT"
                     /locus_tag="IW715_000853"
     CDS             19486..20718
                     /gene="pepT"
                     /locus_tag="IW715_000853"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1021474.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20850..21758
                     /locus_tag="IW715_000854"
     CDS             20850..21758
                     /locus_tag="IW715_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1021475.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21793..22065
                     /locus_tag="IW715_000855"
     CDS             21793..22065
                     /locus_tag="IW715_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1021476.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22076..23122
                     /locus_tag="IW715_000856"
     CDS             22076..23122
                     /locus_tag="IW715_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1021477.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23373..25982
                     /gene="clpB"
                     /locus_tag="IW715_000857"
     CDS             23373..25982
                     /gene="clpB"
                     /locus_tag="IW715_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1021478.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26133..26804)
                     /locus_tag="IW715_000858"
     CDS             complement(26133..26804)
                     /locus_tag="IW715_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021479.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26797..27291)
                     /locus_tag="IW715_000859"
     CDS             complement(26797..27291)
                     /locus_tag="IW715_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1021480.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27311..28531)
                     /locus_tag="IW715_000860"
     CDS             complement(27311..28531)
                     /locus_tag="IW715_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1021481.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28678..30513
                     /gene="lepA"
                     /locus_tag="IW715_000861"
     CDS             28678..30513
                     /gene="lepA"
                     /locus_tag="IW715_000861"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1021482.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30607..31734)
                     /locus_tag="IW715_000862"
     CDS             complement(30607..31734)
                     /locus_tag="IW715_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1021483.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10566 a         5670 c         6793 g         8718 t
ORIGIN      
        1 aagaggtcgt gagcctgccg tttcactctg agtcacaaag agcaatttca aaaaatagct
       61 tctcatgttt gttgaatatt gtgtcttgtg accgaggagt tgtcaggaga acaccgttta
      121 ccagaggttg tgacttgttt ttagtcacga cctctttttt tatttattcc atataaatac
      181 acattattta ttccgaaaag tgaaagcgca aataacaaaa acaaccaact tatttattat
      241 taaagaaaag cgtatttaag taggacgatt ctgttgagag ctttcgtaga ttttcccatt
      301 ttcctatggt aaactgacaa agttgagaat gttagaacag atcgtttagg aggaaacaga
      361 tgtttacgaa tgaaataaaa atcatgcttt atgtggacga tgtagaaaaa aatgcaacgt
      421 tttggagagc agttggcttt gcggaaaaag atcgtcaaga aatggatggg acgcttataa
      481 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      541 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      601 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      661 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      721 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      781 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      841 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      901 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      961 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
     1021 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
     1081 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
     1141 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
     1201 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
     1261 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
     1321 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1381 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1441 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1501 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1561 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1621 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1681 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1741 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1801 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1861 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1921 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1981 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     2041 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     2101 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     2161 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     2221 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     2281 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     2341 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2401 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2461 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2521 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2581 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2641 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2701 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2761 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2821 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2881 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2941 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     3001 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     3061 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     3121 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     3181 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     3241 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     3301 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3361 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3421 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3481 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3541 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3601 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3661 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3721 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3781 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3841 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3901 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3961 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     4021 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     4081 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     4141 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     4201 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     4261 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     4321 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4381 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4441 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4501 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4561 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4621 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4681 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4741 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4801 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4861 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4921 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4981 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     5041 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     5101 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     5161 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     5221 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     5281 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     5341 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5401 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5461 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5521 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5581 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5641 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5701 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5761 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5821 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5881 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5941 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     6001 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     6061 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     6121 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     6181 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     6241 tctcgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     6301 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6361 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6421 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6481 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6541 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6601 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6661 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6721 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6781 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6841 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6901 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6961 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     7021 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     7081 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     7141 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     7201 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     7261 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     7321 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7381 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7441 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7501 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7561 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7621 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7681 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7741 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7801 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7861 gaagctgttt tttcttggct gttcttatta tttgatgcag gacagctttt tcacgacctc
     7921 tgtaaactat aggaatctgt taaatcaagg gagcagaact tgccttttac gctacttttg
     7981 gtacaataat gtatgattaa aaataaactg gagggtttct atcttgaaca taggtaagcc
     8041 gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc gactcgttat ccttaacaga
     8101 atctattgaa gataaccagc ttttgctgta tttgggattg acgaatgatg caaatccttt
     8161 gtatatccaa catgactttg cacaaaaaac agaatatcaa cagccaatcg ttccatcagt
     8221 gatgctgatg gggatcatca caagtgcaat ttcaaagcat cttcctggac caggatcaca
     8281 tgttgtcaat ttttctgtca attttattga accggttttc cattatgaaa cactgacttt
     8341 tcagtttgag gtcataaaag tagataagat gaaggatgtg gtgacgattt ctgtagaagc
     8401 ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt atggtccaac cgccacaagt
     8461 tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg aatgaataat ccaagtacat
     8521 caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg atttttaggt atgggacttg
     8581 cccttagtgc agtcacttct tacttggctc tttgggtatt tcctttgcaa ttagcaactt
     8641 ttatcaataa ttttccacta ggctttgtgg gtctgtggat cgttgaactg attttagtaa
     8701 tggttctagg cgtaaaagca caaaagaatc cgagcttagc aatcggtgga tttattgcct
     8761 actctctttt gaatggcata acattagcgg ttactcttgc atactacgat attggaacag
     8821 taacgcaagc tttcttgaca gctactttga tgttcatggg gatgtctgtt gtcggagtaa
     8881 caacaaaaag agatcttagc ggggttggaa gagcaggaca ggcagcttta atcggagtga
     8941 tcattgcact agtcttgaac atcttcattt tgagaagtag cgtagttgag ctgttcatct
     9001 ccctactgtt agtcgtcatc tttgcaggga ttacagctta tgataaccaa agcatccgta
     9061 atatctatgt ccagacaaac ggaacagcac atagtggaat tgctgtattc atggcattga
     9121 agctgtattt ggactttatc aacttgttct tggcgtttct gagaatcttt ggacggaaca
     9181 actaaaaaag aaaaaaattc atcagttggg gctataccga attatttggt atggtcccgc
     9241 tttttctttt cgcattccgc aggattcttt gttagaatga ataagaactt tacagtaagg
     9301 agttaagaag atgacaaaaa ataaattatt actcgttgat gggaacagtg tggcttttcg
     9361 cgcattcttt gcgcttcaca attctcttga acgtttcaaa aatagaaacg gtcttcatac
     9421 aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt atgcaaaaag agcaaccgac
     9481 tcatgttctg gttgcgttcg atgcaggaaa aacgactttt cggacagaaa tgtatgcgga
     9541 gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa gaacaaatgc catatatacg
     9601 tgatttgttg acaggactag gggtacagta ttacgaattg cccaattatg aagcagatga
     9661 tattatcggt actttggcag aaaaagtaga caaagatcaa tttgatgtcg tcgtgctttc
     9721 aggtgatcgg gatttgactc aattagctag taaagaagta aaagtggata tcactgtaaa
     9781 aggtgtcagc gatatcgaaa gctatacacc agaacatgta gcggaaaaat atgatgggtt
     9841 gacgccaaaa cagattattg acatgaaggg actagccgga gatgcctctg acaatatccc
     9901 cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta ttgaaacaat atggctcagt
     9961 cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc aaaatgaaag aaaatctgat
    10021 aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg accatcaata cttcagcgcc
    10081 tgtggatgtc tctatcgact cattgaagta tgaagggaaa aatctcgata aactcgttcc
    10141 gttttataaa gaaatggatt tcaatcagtt cctttctaaa ttgaatatcg tagaagaaga
    10201 agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa ttcaaagagg aaatgtttac
    10261 tacggatatg gcgttgtacg tggaaatgat gggcgacaat tatcatacag aggaaatcgt
    10321 cggagtagcg tgggggacag aaaagaaaat ttatgtcacg aacgatctat ctgtattcga
    10381 aaaccgagcg ttccattctt ggatcactga tccgactcgg ctgaaaaaag tatatgatgc
    10441 gaaacgaact tatgttgctt taaaccgcta tactggaaaa acgaaaggaa tcgattttga
    10501 tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa agcacagata tcgaaggaat
    10561 agctgctcat tacggataca acgatatcca atcagatgaa gctgtatacg gcaaaggggc
    10621 gaaaaaaggt ttaccagaag aagaggaact cttttttgct catttagcgc gaaaagtagc
    10681 tgcaatcaat gcgttgacac caaaattgag ccaagaactg gcagaaaaaa accaagaaga
    10741 tcttttccgg aaaatggaac tgccattgtc tatcattttg gcagagatgg aaataagcgg
    10801 gatcaaagta gatgcgactc gcttacaaga aatgaagggc gagttctctg ctcgcttaag
    10861 agagatcgaa caaaaaatct acgaagaagc cggcgaagaa ttcaatttga attcaccaaa
    10921 gcaattagga gtgatcttat ttgaaaaaat gggattaccc gttatcaaaa aaacaaaaac
    10981 tggatattcc acagccgtag atgtactgga acagcttcgt gaacaagcac caatcgtaga
    11041 ggatatcctc acctatcgtc aaattgcaaa aattcaatct acctatgtcg agggactgct
    11101 gaaagtgatc caatcagatg gaaaagtgca tacccgttat gtccagacat tgacccaaac
    11161 aggacgatta agctctgttg atcctaattt gcagaacatc ccgattcgat tagatgaagg
    11221 gagaaagata cgtcaggcat ttgttcctag agaaaaagac tggctgattt attcttctga
    11281 ctattcacaa atcgagttac gcgtacttgc gcatatctca aatgatgaac atttgaaagc
    11341 tgccttccta gaaggtcaag atatccatgc cagcacagcg atgcgtgtat ttggaatcga
    11401 aaaggcagag gatgtcacac caaatatgcg taggcaggca aaagcagtga attttggtat
    11461 cgtgtatggc atctctgatt acggactttc acaaaatcta ggaatcagcc gaaaagcagc
    11521 acagcaatac atcgatacgt actttgaaaa atacccaggt gtcaaagaat atatggaaag
    11581 aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg ctgtatcacc gccgtcgata
    11641 tctgccagac atcaattcaa gaaattacaa tatccgttct tttgcggaac gaacagctat
    11701 caatacacca atccaaggaa gtgctgctga tattttgaaa atcgcgatga tcgaattgga
    11761 taaacgatta aaagaaacag gtcttcaagc tacgatgctg ttacaagttc atgatgaact
    11821 tgtcttcgag gtaccggaaa aagaacttga atcattagat aaattggtga aggaagttat
    11881 ggaacaagct gtttctcttc atgtaccact gatcactgac agcagttggg gaaaaacgtg
    11941 gtacgaagca aaataaacat tgtttaagac agagcaatat aatgaaggtg aaaagaaaaa
    12001 atgccagaat taccagaagt agaaaccgtt cgtaaaggat tggaacggtt agtagtagga
    12061 aaaacgattc aaaaagttca ggtcttgtgg cccaagatca tcgagcaacc agagacaccc
    12121 atctttgagg cttctcttgt tggcgaaacc atccaatcca ttggacgtag agggaagttt
    12181 ttaatttttc atctcgatca ttgtgaactg atctcacact tgcgaatgga aggaaaatat
    12241 caatttacga aagaaaatac accaatcgat aaacatacgc atgtactttt cttttttgag
    12301 gatggaagcc aacttcgcta caacgatgta cgaaaatttg gtcgtatgac aatcgtcgaa
    12361 aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg gacctgagcc tttgccagat
    12421 agcttcttat tagcagattt cgcaaatgga ctgaaaaagt cccacaaagc aatcaagcca
    12481 ttgctgctag accaacgtct agtgactggt ctaggtaata tttatgtaga tgaagcatta
    12541 tgggaagcga agatccatcc agaacaaccg gcgaacacac tgcgccccaa agaggtagaa
    12601 caattacgtc tatcgatcat tgatgttttg gatcgtgcaa tagaagccgg aggtaccacg
    12661 attcgcagtt atttgaatgc attaggagaa tcaggcggat ttcaagtagc actgcatgtc
    12721 tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc cgatcgtgaa aacaaaagtg
    12781 gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc taaaaatcgc aaagtaggat
    12841 ttgttttagg tttgacgggt ggaatagcta ccggtaaaag tacggcagcc aaagtctttc
    12901 agtctcatgg atttcctttg atcgatggcg atgtgattgc cagagaaact gttgagcctg
    12961 gaacgcttgc tctgaggaaa attgtatcag tgtttggcca agaggtattg cagcctgatg
    13021 ggcaactaga tcgagggaaa cttgggatga ttgtttttcc atcaaaggaa aaacggcaaa
    13081 aacttgatca gctattagat ccgtttatcc gaaaagcgat aaaagatcaa atcgcctctc
    13141 taagttccaa tcatccttta gtaattgtag atatcccatt gctctacgaa ggtcattatg
    13201 accactatat ggatgcagta gctgtcgttt atactacgcc agaaactcag cttcagcgtt
    13261 tgatgaaacg taatcaactg acgctagaac aagcgcagca gagaattagt agtcaacttc
    13321 caatcgaaga aaaaaagcag cgtgcggata ttttgttcga caacaatgga acaaaagaaa
    13381 aacttgtggg acaaatcgaa aattggcttt ttgctaatcg ctttctttga gtatctgtgt
    13441 caattttgct atcctgagta tatagaaagt gagggatagc gatgggatca ttaaaaaatc
    13501 aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg cgagatgctc aataaagaaa
    13561 atctaatcga tgaaggtcgt acgcaatcag aaacagcaga agaaaaagaa aagcagtacc
    13621 aagcaagaat cgatgaaata gaagaccagc aggcagaaga agaagcgaag cggcaagatt
    13681 tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa aacaccgctc ccagaaagtg
    13741 accacattcc cgatgagcaa aaaatcgagg aacagcgaat gaaacactat acaggaatcg
    13801 aagagaagtt ataaaatagt aaaaaatcga gatgtgactt gaagcagatg agaaggtgtt
    13861 catctgcttc aagttttttg tgctagatga ctaaatcagc ttttgtgtaa gttatttctc
    13921 agtaatcaag gtgatcagta ttggaaaatt gaaatttagt aaggacaggg gacacaatta
    13981 cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt ttgggtagga cagtcgcgtt
    14041 tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga aagacacatc ctttatataa
    14101 aatgcgatgc aagaggtgag acaaaatgag atgtcccaga tgtcaacata ataattcacg
    14161 agtaatcgac agccgtcaag cagatgatgg acgtgcgatc cgtcgccgtc gcgaatgtga
    14221 gaattgttcg ttccgattca ctacatttga acgaattgaa gctgctccgt tattagtaat
    14281 caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa attttaagag gactcatccg
    14341 ttcagcagaa aaaagacctg tagctatgga acaaatggaa caaatcgtca accatgtcga
    14401 aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg aatctaattg gtgaatatgt
    14461 gatggaagat ttagttgatt tagacgaaat cgcttatatt cgttttgcaa gtgtttatcg
    14521 ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa gatatcgtgg ataaagcaaa
    14581 agaaaataat aaagagaata ataaagaaaa caattaagaa acaaaaagga ggcatttcct
    14641 tggaaaatgc ttggaaagag cttcaaccaa aaaatattta ccaaggaaga aaagcggctc
    14701 cgctgagaga agacagcaag aacgccttgc tgtatcttta tcagccacta atcggcggag
    14761 aagccttgag tatctatttc accctgttat cagaaatctc tcaagaatct ggcaaaggtc
    14821 ctgaagggat gcatgcagat ctgcttagta gtctaggctg cggtatcccg caattttatg
    14881 atgcaagaaa aaagctagaa ggaatcggct tactggatgt ttattttaaa gaagatagtg
    14941 atctgggacc tcgttttatt tatgagctgt tagaaccact cattcctaaa gcatttttca
    15001 aagactctgt tctggctttt cttcttctgg aaaaagttgg tgaacgacga ttccagaagc
    15061 ttacccaatt ctttgaacct aaaaggcata cagcagaggg atatcaaaaa atcacaaaaa
    15121 aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc tgctaatcaa gaaaacatcg
    15181 aatcgctgaa taaatctttt gaagaatctg aatcagtagc attacttgaa gcagattcga
    15241 cattcgattg gcagtttttg aatgattggc tggcaaaaaa acatattgca agtcttgatg
    15301 atccaatcat caaacaagtc gacatgtatc atcagcttta cggtttcgat gaattgacac
    15361 ttggcgaact gatcagtcaa tcttatgact ttagtgaaca aaaagtatct gaaaaagaac
    15421 tgcaaaaagc cattttggca atgcagagac gtccggtgaa gcaaacaaaa gaaacagttg
    15481 cggtttctga aaacagtaat gaaccctcgt caaacgaaca tttgacaaac gcagcgcttc
    15541 agctgataaa agaagcacag agcattccac cgatgaaata tctggaagca atcaagtcag
    15601 aaaaaggcgg atatgtttct aaacaagaaa actggttact gcaagatctt gtttctcaat
    15661 caggtctttc cagtagcgtg atcaatgttt tgctgaatta tgtactagtg atcaagaata
    15721 atgctagctt aaatgccagt tatgtaaata cagtggcaaa tgaatgggca caaaagaaaa
    15781 tcgccacagc agaagaagcg atccagcaca ttcggcaaat aagtaatcaa gcaaaacaat
    15841 caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg aaatgttcga cgggaaaagc
    15901 ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat cagcagcgag aaaaaagcag
    15961 agattgaccg ccgttttaaa gaatacctgg caaaaaagga gggtgatatc tagtggaaga
    16021 cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat tatcaagaaa agtatgctga
    16081 aatgatcgct gaagtcttaa atgatgcgga tgttcaagct tttctaagcg cacaccaaga
    16141 agaattgaca caagcggata ttgaaaaaag ttacgctaaa ttgtatgaat tcgtgcaaga
    16201 aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct cccggctatg agccgaagct
    16261 gacgttgaat tttcattatg tggatgtcac ttatgtacca acggaagcat tgttagcacg
    16321 gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg gatatgccaa aagatattca
    16381 atcggcttct tttgaaaact ttgaacgtac agatggacga ggatatgcga gcttagaagc
    16441 cctagatttt gtagagaaat atactgctga tccaaaaggg tatcataaag gactgtatct
    16501 agtaggaagt tttggtattg gaaaaacata tttattagga gcaacagcga gagaattagc
    16561 gattgctgga ttcaccacga cgctagtcca ttttccgaca tttgccgttg agatgaaaca
    16621 ggcaatcgga aaagatcaag tggcagaaaa gttagatgca gtgaaacgtt cacctatttt
    16681 gatgatcgat gacattggtg cagatgcgat gagtagttgg atacgagacg aagtattcgg
    16741 tgttatcctt caatatcgga tgcaggaaca gttaccaacc ttcttctcat cgaattttac
    16801 aatggatgaa ttggaacagc atttgtccgt cactcaacgt ggagatgaag agccgctaaa
    16861 agcgaaacgg ataatggaac gcatacgtta tttgacaaaa gaaatcgaaa tgactggtcg
    16921 caaccgacgc aatggttgaa cagttgacaa gtccaactga catttgacct agtagtaaaa
    16981 gcaggtcagc gaatcattat atggacagaa atgaccggag acaatcaccg caacaagtaa
    17041 tgcagaaact taaacggagg attttttatg agggctattt tttctagtat atctgtagtt
    17101 ggtctatttt ttcttttagc aggctgcggt agcgcagata gcaacacagg ttctgttgat
    17161 tcgattgatg cgggtcaaac agtaaaaaca agcagtacga aaacggagag acaaagcata
    17221 tttgtcggaa cgattgaaag tattactcaa gtagaagcag aagaagaaac gcttcagttg
    17281 aaactcgtcg atgtcatggc caaagaagat ccagcgaata tgggaacgtc attcagcaat
    17341 gacggagtga tcttaaatgc tactgctgaa aaaataagcg tggacataaa tgaattgaaa
    17401 atcggtgaca aagtccaatt tacattagtt gagatgccga tcatgacgat gtctatccca
    17461 ccgcaagttc ctggagattc gattattgat gtttcattga acaaataacc aataacattt
    17521 tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg tatatttttt aaggagatat
    17581 gggaattttt ttattcctag tctccttatt ttatttggaa aatgctataa ttatctagaa
    17641 ggagtgaagc tatgaaccat ttagatttat ctcagcgctt agccgcagtg ggtgactttg
    17701 tccctgaatc cgctcgttta gcagacattg gatcagatca cgcttactta cccgttgcat
    17761 tgatattaag aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa ggtccttatg
    17821 aatcagcacg taagcaagtc aaaaaagatg gattagaaga ccgtatcacc gtacgccttg
    17881 caaatggtct agatgctatc cagccacaag atggtattac agctgtcacg attgcgggta
    17941 tgggaggttc actgatccga gatatcctag aagctggcaa gttgaatcaa cgcttaagcg
    18001 gacaagaacg tttgatcctt cagccaaaca tcggagagcg tacattacga tcttggctgc
    18061 aagaaaatca gtataaaatc caatcagaaa aaatacttga agaaaataaa aaaatatatg
    18121 aaatcattgt agccgaaaaa gaacaaaaac cagttactta caatgaaatg gagctgatgt
    18181 tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg acaagagagc
    18241 ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa gaggcacgtc
    18301 aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt tttaaagcca tgacaatcag
    18361 cggacgcact ttcatccaaa agtttgaaag ctattgtcct cagtggctag ctgaagaagg
    18421 agacccagtc ggactgcata tcggaacatt ggataagcca atccaacgag taatgatgac
    18481 attagacgtt cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg atttgctgat
    18541 tgctaaacat cctccaatct ttcgtcctgt aaaacggctg gttactgatc agccacaaga
    18601 aaaaatgtat gcagatttgt tgaaacatga tattgccgtc tatgcagcac acacgaacat
    18661 ggatattatt tgggatggac tgaatgactg gttttgtgaa cttctcggta ttgaagtgga
    18721 gagttattta gtaaagactc atgagattca ttataaaaaa ctggccgttt atgtacctgt
    18781 ggatcatgca cagaaaatgc gtgaagtatt agctgcagct ggtgctggta cacaaggtga
    18841 ttataccggt acaagtttta cctcgattgg acacggtcga tttactccgg aagctggtgc
    18901 acaaccagcg attgggaaag tagggaagac agaacaagtt caagaggcaa aagttgaggt
    18961 tattttgccg gaaactatcg aaaaacaagt gattcaagcc atgcggtcag ctcatcctta
    19021 tgaggaacct gcttatgatc ttttcgctat agatgaacca gtcgaaatgt ttgggttagg
    19081 acgagtaggt gaattgccgc aagaaatttc tatagaggca tttgtagaac aagtaaaaga
    19141 agcgttccaa ctagatggac tgagaatagt ccagccaaaa aatgcaaaat cctctgtgaa
    19201 gcgcattgcg atttgcggtg gaagcggtga aaaattttat ccgcaagcaa tcgcccaaag
    19261 agcagatgtc tatatcacgg gagatatcta ttatcatacg gctcatgata tgcaaagcgc
    19321 tgggttgatt gcaattgatc ccggtcatta tattgaatcg ttatgcaagc aacgatttat
    19381 cgagaaattt gaatcttgga aacaagaaga aaattgggat attaactttt tcgtttctga
    19441 aacaaataca aacccatttc aatttaatta aaaggagaga atactatgta cgaaaatctt
    19501 ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac aagcaaaacg
    19561 actccatcga ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga attagaagag
    19621 ttaggcttag ctgatgtttt ctataatgaa gataacggat tcgttatggg aacattacca
    19681 agcaatgttg atcatgaagt gcgttccatt ggatttattt cccacatgga tacagcagat
    19741 tttaatgcag taggagtcaa tccacaaatc attgaaaact atgatggcga gtctacgatc
    19801 gtactagatg cggaagaacg ctatacattg aatacaaaag attttccaaa tttgaaaaat
    19861 tatgaaggcc acacattgat cacaacagat ggcagtacat tactgggagc agatgacaaa
    19921 tcagggattg cggaaatcat gactgcaatg gagatcttga tcaataatcc ttctatccct
    19981 cacggtacca tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg agcggataaa
    20041 ttcgatgttg agcaattcga tgtagatttt gcttatacag tcgatggtgg tcctttaggt
    20101 gagttgcagt atgaaacgtt tagtgctgct caagcaaata ttacgatcca agggaaaaat
    20161 gtacatccag ggacggcaaa agatacaatg atcaatgctc tacagttagc aatcgatttt
    20221 cataatcagc ttccagccga tgaagttcca gaaaaaacgg aaggatacga aggtttcttc
    20281 catttgatgg cattggatgg gaatcctgaa gaagcttcta tgagttatat cattcgcgat
    20341 catgatcgtg aaaaattcga agcacgcaaa gcacaaatca cacatatcca agaagagttg
    20401 aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt atgatcaata ttacaacatg
    20461 aaagaaatca ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc aatgatcgag
    20521 cttggtatct ctccagtgat cgaaccagtt cgtggaggaa cagatggttc aaaaatctct
    20581 tacatgggta ttccgacgcc aaatctattt gccggaggag aaaatatgca cggacgattc
    20641 gaatttgttt ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa aattgcagaa
    20701 ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt cgtcccaatg
    20761 atggaatgtc atctgtctaa ggctaaaggg caggacaatg gaattggttg tcccgccctc
    20821 ttttttgaag agtaaatagg aggtaaagaa tggaaaagct gctagttttt tataacaaaa
    20881 gttcagggaa agatgagggt gagaaattag cacgctggtt tcaagattat gtaaaagaaa
    20941 agcagccaga attatatgta gaactagctg aaactggccc atcgattgat gatgcggaat
    21001 tagtcaagaa agcagaagcc atagaagctg atactttagt aattataggt ggagacggca
    21061 cgattcatca tatcgtccaa gcgttccaaa aaacattatc tcaataccat gtcggattac
    21121 ttccaggtgg aactgtcaac aaccttgcac gtgttcttga gattcccctc aaaaaagaag
    21181 aagctgctga cgtgatcctg gaaggcaatg tacggaaaat agactatggt caagtcaatg
    21241 atgaagtcat tatctctaca ctgacaatcg gaatcttagc agatacagca gtttggatca
    21301 gtcaagaaga aaaacagcgt tacggctcgt ggatctttat caagaagttt tttaagttat
    21361 tgctgaagaa gaaaaaatat catttggatt tagaaacaga cgaggagcat tggaaaggaa
    21421 aatcccaact gttgacagtc acgatgtcta actccgtggg cggttttaca aattttgacg
    21481 actcagcgac cctagatgat ggaaaatttc atgtaacgat cgtaccttcc ttagatattt
    21541 tccgatttat tttttatttg cctagaatca tgagaggcaa attctatacg atccccggca
    21601 ttacttattt cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct gtgggcactc
    21661 gaacggatgg agacacgaca gatgatcttc cagttgaatt atctgttgta tcaaaaggat
    21721 taacagttta ttcaccaaag aaagaagaaa aagcttagaa atagtagtta acagaaagtt
    21781 ggaagaagag taatggatgt attgttacac aatcttttgt ggttttacct tttcattgtg
    21841 aatttttact tattttgtct gatggggtat gataaacatc aggctagaac tagcggctgg
    21901 cgtgttcctg agaataattt gatatttgtc agcctgttag gcggtggttt gggaggattg
    21961 atctctcgaa aagttttcca tcataaaact cggaaaaaga agtttactat aggattcgct
    22021 gcaggtgtac ttgttgattt gattctcatt tattttttcc attaaggagc aggagatgtc
    22081 gtctaaagga acgaaaacta aaatcattct gaatctgctt ttgctgattg tgatttttgc
    22141 cattatttat tatttaatca atcaatcatt tgcagatatc tttgccgaat tgctgtctac
    22201 cagtgttgtt gttttggcag cggctgttat attagggaca gtttaccaaa tcgctgaagg
    22261 gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat tttacaacga aagatgggtt
    22321 cttgacctct agctatattg cgttttaccg aatcgtttcg tttggtacag gaacgctgat
    22381 ctcggagatt tatttttata acaaaaaagg aatgaagcta tcccagggag caggggtgac
    22441 agcactccat atgattatgt acaaactggc agtgatcact tatgcattag ctggcttggt
    22501 catccaattc tctctttttt attctaatgg tccatcaatg atctggttta tcatcgctgg
    22561 tatccttttg acgatcgtga ttgttgcctt tttattggcg atgtccgtca gcttgaatgt
    22621 tcaaatattt tttgtcagct tatcgaataa gttatttaaa agtgagcgtt tgcggaaatg
    22681 ggtggataca tgcaatcagc agatctactc tttaagggaa acggtccaaa ccattatcca
    22741 agacaggact gcgctattgc ggatttatct atggaatatg ttaaaacttg tattttggta
    22801 tgtacttcct tatttgattc tgattgaaaa ccatcctaaa atagattttc tactgaccat
    22861 ttcgtttatc agctttgcag tagtgctttc aggggttatt cctacgcctg ctggtattgg
    22921 ttcctttgaa tttgtctatt tgttattatt cagaccagtt gttggaacag ttgacgcagt
    22981 ttcttcgctt ttactgtatc gtttcagttc ctttatcttg ccatttctct atggatttgt
    23041 gtatgtgatt gctgaacggc gaagagtgat caaagaagaa ataatagaag taaaaaaaga
    23101 gaataaagaa aatcaagggt aggtctttta taagatctac cctttttatc aaatcaaaga
    23161 aaaacgttat ggataagata attttacgtt tgtttggctc tttttttata tacatacaat
    23221 caattttgtt tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa ccatgtgtat
    23281 attttgacca aaattgacca aagcgatata atagtttttg taaagatcaa agaaataagt
    23341 gatcttacct ttaattagga gggtatacct gtatgaatat tgagaaaatg actacgacgc
    23401 ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat caagatatcg
    23461 acattgccca tgtatggaaa atctttttac aacctaatca ttttggtcga aatttctata
    23521 cggatgccgg attggatgtc gaatcttttg aacatgaaat tgatcgtcta ctggatgagt
    23581 atccagttgt ttctggtgga aacgtccaat atggacaaaa cctcagccag aatttctttc
    23641 gattattgaa tgaagcagac caaatacgtg aaagcttcgg cgacgagttt ctttcaacgg
    23701 aagtcgttat tttagcgttg atgaaattaa aaaattatcc gttaactgtt tatttgaata
    23761 aaaacggatt atctgaaaaa gaattgcgta aaaacatcga agagatgaga ggaggagaaa
    23821 gagtgacctc acaaaaccaa gaagaacaat acaaagcatt agaaaaatac ggcgttgatt
    23881 tagtacagca agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat gaagaaatcc
    23941 gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg ataggagaac
    24001 ccggtgtagg gaaaacggca atcgttgaag ggcttgctca acgtatcgta cgcaaagatg
    24061 tgcctgaaaa tttaaaagat aaaacgattt tttcattaga tatgggtgca ttgatcgctg
    24121 gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa gaagtcaaaa
    24181 aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca caatatcgta ggggcaggaa
    24241 aaacagaagg aagtatggat gccggaaatc tgttgaaacc aatgcttgct cgcggtgaat
    24301 tacatttgat cggtgccaca actttagatg agtaccgtca atatatggaa aaagacaaag
    24361 cgttggaacg tcgctttcaa aaggtattag tcaaagaacc aacagtggaa gatacgatca
    24421 gtattctgcg tggattaaaa gaacgttttg agatccacca tggtgttaac atccatgaca
    24481 acgcattagt agcagcagct actttatctg accgttatat tactgatcgg ttcttaccgg
    24541 acaaagcaat cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa atgaactcta
    24601 tgccaacgga attagatcaa gttactagac gtttgatgca gttggaaatc gaagaagctg
    24661 cactgaaaaa agaatcagat gatgcaagta aaaaacgctt gaaaaatttg caagaagaat
    24721 tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca gaaaaagaag
    24781 aagtcaattc tgtttcagca aaacgtgctg aaatcgataa agcaaaacac gaattagaag
    24841 atgcagaaaa taactatgat ttagaacgcg ctgctgtatt gcgtcatggt acgatacctc
    24901 aattagaaaa agaattgaag gaattagaag ctaaagcaaa agacagcgag attaagatgg
    24961 ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt tggcagatta acgggtatcc
    25021 ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat taaattgaat gaaacacttc
    25081 ataaacgagt gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg gtaattcgtt
    25141 cacgggctgg attgcaagac ccaaatcgtc ctctaggttc tttccttttc ctaggaccaa
    25201 ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt gactcagaag
    25261 atcatatggt acggatcgat atgagtgaat atatggaaaa acatgctgtg tctcgtctag
    25321 tcggagctcc tccaggctat gtaggttacg aagaaggtgg tcaattaacg gaagctgtca
    25381 gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat cccgatgtct
    25441 tcaatatctt gttacaagtc ctagatgacg gacgcttgac tgattcaaaa gggcgagtag
    25501 tagatttcaa aaatacggtt ctgatcatga ccagcaatat cggttcacag ttactgctgg
    25561 aaggcgtgac tgctgatgga acgattccag aagcagtggc tgaacaagtc aacacgctat
    25621 tacgtggaaa cttcaaacca gaattcttga accgaatcga cgacacgatt ttatttacac
    25681 cattgagcct agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa ttggcacaac
    25741 gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg tggattgctg
    25801 aaaatgctta tgaaccagca tatggtgcaa gaccattgaa acgatttatc actaaggaag
    25861 tagaaacacc actagctaaa gaaatcgttg caggacatgt tatgcctaaa tcaaaagtaa
    25921 cgattacttt acttgatgga caacttcatt tcaaaacaga agagctagaa gaaatcgtct
    25981 aatcttccat tgagactata aaagaaaaaa ggggtctgac acaactagtg tcagacctcc
    26041 ttttttcaaa taaacagtgt tccagaagta gtttcttcga aaaataagcc gaaatttctt
    26101 gaagctgccc gcttctgtca caacctcttt tattaggtat ttttcacttt ttctaattta
    26161 taactttcaa agtaacgctc atctttttta ctttgagacc atgcaaacaa tcccattaag
    26221 gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc tgctcgtccg
    26281 acaaacagtg tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa gaactggtcg
    26341 cataagaatt cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc aaagccgaat
    26401 aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa gtaaccaatc
    26461 aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat tttgtcccaa
    26521 aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat gaacaagaaa
    26581 aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt caaaatgact
    26641 tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt gattccttga
    26701 gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac gaatacacca
    26761 aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag tccctccatc
    26821 taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt ttccgttcaa
    26881 tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag ctgtttcttg
    26941 tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag tatataagcc
    27001 gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc cggcatcgat
    27061 cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt ctgtgtcagc
    27121 aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt cattttcttg
    27181 aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta actgttggtt
    27241 atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca tctaaacatc
    27301 atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg atacgctcta
    27361 gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa taagaagcac
    27421 ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg gattttccta
    27481 taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg aagacaccat
    27541 tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct gttccaacaa
    27601 acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa tatggttcga
    27661 tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt ttataaatat
    27721 ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat aagccatttc
    27781 gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca gctacccagt
    27841 agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct gtgcgctcat
    27901 cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat aaagcaccat
    27961 ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca actggtaatc
    28021 gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg attttaaagg
    28081 aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata ctgtaaggat
    28141 acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg ataattagat
    28201 cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc acaccaccca
    28261 ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt tgagagatca
    28321 gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt tttttggaac
    28381 ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca gtcacttgga
    28441 acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc ggttttaatt
    28501 caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt agcttgataa
    28561 cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa actctttaaa
    28621 ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg agacgctatg
    28681 gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat catcgctcat
    28741 atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca cactgtgacg
    28801 tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga acgtggaatc
    28861 acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga aacatatatc
    28921 tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc tcgaagcctt
    28981 gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga agcacaaaca
    29041 ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt catcaataaa
    29101 atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga cgtaatcgga
    29161 atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat tgaagatatt
    29221 ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc accgctaaaa
    29281 gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa tgtccggatc
    29341 acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg caaaacattt
    29401 gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt tttgatggtc
    29461 ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg agtaggggat
    29521 acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg aaaaatgaat
    29581 ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga tttgcgggaa
    29641 gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga aacctctcaa
    29701 gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga tgtcgtccaa
    29761 gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc cgttatttac
    29821 catgttaata aaacggatgg tacgactgct acagtagata acccggcaga ttttcctgaa
    29881 cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat ggtcccaaat
    29941 gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt cattacgatg
    30001 gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc tgaaattgtc
    30061 tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt ggattatgaa
    30121 atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa tggagaaaaa
    30181 gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg aaaagcaatc
    30241 gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat ccaagcagct
    30301 attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa cgtcttggct
    30361 aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca aaaagaaggg
    30421 aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt catggccgtt
    30481 ctgaaaatgg acgaggacga accgaagaaa tagcaagggt ttgtttgaaa acagtgtaaa
    30541 aaataaacca tgtttacatg gatttgccac cggtttgcca ccggtggttt ttttcggtgg
    30601 caaaaatcaa gaattctttc taagattttc catgatatcg accgtttcat ttctcatctt
    30661 atctgttaca tgcgagtaag tatccatcgt aattgaaatt ctgctgtggc ccaatcgttc
    30721 agagatttct ttcatttttg cgccattttc gagaagaagt gtagcatgag tatgtctgag
    30781 agaatggaag ttaaaagaga gggagagtgc attcgatatc ctcctagtat tccatttcac
    30841 tacacttggc gtaactagct caccgtcttc cttcgtacat actgcatttg aatcaatgta
    30901 gagctttcca tacttcattc gattttctaa ttgttgtttc ttatgttttt tcagaattgc
    30961 tagcaaggtt tgtccaataa aaatcgttcg attggaactg cttgtctttg gcgtaccata
    31021 tacccatgcg ccgtcattct ttaccatttg tttctctaca gtaattgttc catttgaaaa
    31081 atcgacatca tcccacgtca gaccacaaac ttcgccaacg cgcattcccg tataaaatcc
    31141 aatattcaaa ggaatataga aaggatgtcc ttcaggagta atttctagca tatgatcaaa
    31201 gtcctcaaga gaaatgattt ttagatcttt tttagtcgtt ggtcgtgctt catattttgg
    31261 tatttttaca tacagcatag gattttgctt gattaatccc caaggataaa ccgccatatt
    31321 cagcgcattc ttaaggacag agtgagtaat agtcattgtt ttcttcgagt aacccttttt
    31381 aaattcagca ttgatgaaat tttgtaaaag agcaggggag agatccgtaa gttttttctt
    31441 tcctaaataa ccgtttatat gatttttgat ggtaaatcgg tagttttcat aggtattgta
    31501 ttttagattt agtttaacgt attcctccat ccaaaaatca aggtattgtt ttactcgagt
    31561 atccgtacct aaaaagtatt gtcctgtttc gtcaatatct gataaaactt ttcgtaaagc
    31621 agcttcggcc tctggtcggg tgtctccgcc aactttctcc actttttttc ttgagccatc
    31681 atcattgata tcttcgccgt ttgtataatt aagcgagaca aataaaaaag ccatttatgt
    31741 tacactc
//
LOCUS       DACEMK010000022        30988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEMK010000022 DACEMK010000000
VERSION     DACEMK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.022"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            32..271
                     /locus_tag="IW715_000863"
     CDS             32..271
                     /locus_tag="IW715_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021484.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            398..1087
                     /locus_tag="IW715_000864"
     CDS             398..1087
                     /locus_tag="IW715_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1021485.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            1093..2259
                     /locus_tag="IW715_000865"
     CDS             1093..2259
                     /locus_tag="IW715_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1021486.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            2341..3012
                     /locus_tag="IW715_000866"
     CDS             2341..3012
                     /locus_tag="IW715_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1021487.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3065..5041
                     /locus_tag="IW715_000867"
     CDS             3065..5041
                     /locus_tag="IW715_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1021488.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            5054..5806
                     /locus_tag="IW715_000868"
     CDS             5054..5806
                     /locus_tag="IW715_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1021489.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            5822..6583
                     /locus_tag="IW715_000869"
     CDS             5822..6583
                     /locus_tag="IW715_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021490.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            6596..6856
                     /locus_tag="IW715_000870"
     CDS             6596..6856
                     /locus_tag="IW715_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021491.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            6853..8943
                     /locus_tag="IW715_000871"
     CDS             6853..8943
                     /locus_tag="IW715_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1021492.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(8979..11036)
                     /locus_tag="IW715_000872"
     CDS             complement(8979..11036)
                     /locus_tag="IW715_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1021493.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(11181..11372)
                     /locus_tag="IW715_000873"
     CDS             complement(11181..11372)
                     /locus_tag="IW715_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021494.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            11560..12015
                     /locus_tag="IW715_000874"
     CDS             11560..12015
                     /locus_tag="IW715_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021495.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            12012..12254
                     /locus_tag="IW715_000875"
     CDS             12012..12254
                     /locus_tag="IW715_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021496.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            12272..12574
                     /locus_tag="IW715_000876"
     CDS             12272..12574
                     /locus_tag="IW715_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021497.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            12643..14697
                     /locus_tag="IW715_000877"
     CDS             12643..14697
                     /locus_tag="IW715_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021498.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            14697..17309
                     /locus_tag="IW715_000878"
     CDS             14697..17309
                     /locus_tag="IW715_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1021499.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            17306..19684
                     /locus_tag="IW715_000879"
     CDS             17306..19684
                     /locus_tag="IW715_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021500.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            19745..20287
                     /locus_tag="IW715_000880"
     CDS             19745..20287
                     /locus_tag="IW715_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021501.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            20365..20697
                     /locus_tag="IW715_000881"
     CDS             20365..20697
                     /locus_tag="IW715_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021502.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            20698..21291
                     /locus_tag="IW715_000882"
     CDS             20698..21291
                     /locus_tag="IW715_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021503.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            21313..23337
                     /locus_tag="IW715_000883"
     CDS             21313..23337
                     /locus_tag="IW715_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1021504.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            23337..24536
                     /locus_tag="IW715_000884"
     CDS             23337..24536
                     /locus_tag="IW715_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1021505.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            24552..25196
                     /locus_tag="IW715_000885"
     CDS             24552..25196
                     /locus_tag="IW715_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021506.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            25207..26013
                     /locus_tag="IW715_000886"
     CDS             25207..26013
                     /locus_tag="IW715_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021507.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            26036..26265
                     /locus_tag="IW715_000887"
                     /pseudo
     CDS             26036..26265
                     /locus_tag="IW715_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26424..27359
                     /locus_tag="IW715_000888"
     CDS             26424..27359
                     /locus_tag="IW715_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1021508.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            27403..27672
                     /locus_tag="IW715_000889"
     CDS             27403..27672
                     /locus_tag="IW715_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021509.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            27948..28223
                     /locus_tag="IW715_000890"
     CDS             27948..28223
                     /locus_tag="IW715_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021510.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            28265..28687
                     /locus_tag="IW715_000891"
     CDS             28265..28687
                     /locus_tag="IW715_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021511.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            28707..30299
                     /locus_tag="IW715_000892"
     CDS             28707..30299
                     /locus_tag="IW715_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021512.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30320..30655
                     /locus_tag="IW715_000893"
     CDS             30320..30655
                     /locus_tag="IW715_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021513.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            30801..>30988
                     /locus_tag="IW715_000894"
     CDS             30801..>30988
                     /locus_tag="IW715_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021514.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11145 a         4822 c         6297 g         8724 t
ORIGIN      
        1 tttgcaaaaa acaagaaagg taggaaaact catgaattat gaagaaatcg aaaacagaaa
       61 aaaagtgtcg aaagaaatgg aagaaaagtt gctaaaaatg atgaaacaaa aacaccttaa
      121 aagattatct gtcatgcaat atatcaatga tatgaagata acaggaaaag agaaagcttg
      181 tttgttaggt tcaatgaaaa actttgaaca gttgaggcga acttatgtaa aaacaggttc
      241 caattgccaa ctattattag aagtttcttg acgattatga tatgaaaatg atcaattatg
      301 atgtggcatt gttattgaat aaaaagaata gtaaaattta acatggttta tcttggttag
      361 atgtaggtaa attaattcat atatagaagg tagatagatg aataaaaaca ataaaacttt
      421 atttaatcct tacgtgggcg taatcattgt ttttcttctt tttagttatt ttgcttatca
      481 aggttatcaa aaggacaaaa tcgctgaaaa agtagatggt gtaacaaaaa caatccatat
      541 tcaggaacag gctaaaacct atcaagataa tttaaccagt ttaacggata tttctaagag
      601 acaactgatt actaatcaaa aacaggcatt agaaaaggat ctcaaaggtg aaattgctgg
      661 aaaacttgtt ttttcttcta caggatttat tctccctgtt tttcaaggag cgaatagcga
      721 cacgcttagt ttaggagtag cttctactta ttatctagat actgaaatgg gaaatggaaa
      781 ttatattctt gctggttata acgtagatac accaagcgtt ttattatccg atatgactga
      841 tttatctgaa ggagaagaaa ttgatctaat cgatgctcat tacaagtatc agtatgaagt
      901 ctccaaaaga ggaaaaattt ctgattacag ccaggtagtt caatctaagg aaaacaattc
      961 ctttttatcc ttgccaaaag caggtaaaaa accactactc acactgttag cctttcaagc
     1021 aaataatatg aaaaagtgcg atgtgatcca gtgtgaatta gtcaatgtag aaaaaatcaa
     1081 taaataaggg aagtgagtca cattcaagaa cttgtagaaa aattgatcaa taaaatgagt
     1141 gccatttttc tgttactgtt ggtatccatt ctatatttaa tatggacggt ggggttaaat
     1201 cctcctacga tattatttat ttatgataat gctgcacagt tagtgacgtt tttcgtaggc
     1261 tgtttgtttc ttttgaatct gtacaaggta aagagaaacg atctattctt cattgcagca
     1321 ggcattcttt tttacctttt cttttctttt tttagaagct accgaggaga tactttctat
     1381 ggtgatctga tcgttccgac aattattgct attattttga ttgtaaaatg gacgaaattt
     1441 actgtaattg atcgtagctt gtatcttgta gtattctatg tctttttact cgtaacggtt
     1501 tatcgagtat ttactgagat accagtacca gaaggacaaa gtatttggaa tccatcgaac
     1561 aaattatcag atatttggat caatacaaat acgataggta gttctttaat gacattagcc
     1621 cttcttatca caggatttgc tagttcattt ggaaaatggt acatgcgatt gctatcatta
     1681 ccagcgttga ttcttgctgg ggcaacgatt tgggtttgcc aatcgaaagc agcattatat
     1741 gcgcttgtta tcttcattat cttagaaatt ttacctaaga aggtttttcg aaatacacac
     1801 ttgtcctttt ggatgtatgc agcagtttcg gttctagctt taccgatttc ttattttgct
     1861 gcaatgtcag acaaagttca cttatttaca ggacgagaag aaatttggcg caaattctat
     1921 gaaacattag gcgaaaaaac aggacaaata tttgtaggga tgaaaccttt ctttttccat
     1981 agaggaaatc agattctagg taatcataat acgtatcacg cactcgtcaa tcactatgga
     2041 ttgattggtt ttgttctatc gattattctt ttgcttagtt tcttatggtt gctcgtaaga
     2101 aaacgtgagc tgacaaatgg acaactaaca ttcttgtggg cttttttagc gatcatgtgt
     2161 caatcgttca tggaagaaac attaacgaca atcagttggg tgccaattgt attcttaatg
     2221 ttagcaatgg ctacacacca atataaaacc accagataaa tgaaatagaa tagtaataag
     2281 agaaaacact ttttcgttgt gaaaaagtgt tttttgtttt gtctgaaagg aggcagatgg
     2341 atgaaaaaat ggttatttgg ttttttagga gtcgctttaa tcgtcgtttg cagtgtgttt
     2401 ggctatgtta gctaccaaaa acatgaagga gaggtgttta agcaaaatat agaaaaaaag
     2461 atgcccgtag atcaaatcaa tgcacacgca aagagttata aggaggatgc aacgaacgtc
     2521 aataatgata tgtctttggg gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc
     2581 gtcaataaac aagtatttgc acaaattcag attcctgcac ttggtttagc attgccaatt
     2641 tttaaagggg caaatcaata cactttgagt cttggagcgg ctacttattt ttatgaagat
     2701 gccgaaatgg gtaaaggaaa ctatgtccta gcaggtcata acatggaaat gccaggtgtt
     2761 ttattttctg atattcaaaa gttgtcatta ggggaggtga tggacttagt tagtaacgat
     2821 ggcgtctatc gttacaaggt aacgcgtaaa tttatcgtac ctgagtactt taaattaatc
     2881 gatggcgttc ctgaagagaa ctctttcttg tctttaccaa agaaaggaga gaaaccctta
     2941 ttaacgttgt ttacttgtgt ttatacgtca caaggaaaag aaagatacgt ggtacaggga
     3001 gagctacaat aacaagaatt taatcaacgc gagtcgttga aatatgaaat gaggagagta
     3061 aaaaatggca ataaatcggt atgtaaaaaa agctggaatg attgttggaa tgttaggaat
     3121 cattgcaggc tgctttcagg ggtatcgagc ttatgcagaa gatgtcacgc aaaaaacgcc
     3181 accagaaagg gtcaatatca cggttcataa attaatgtac gatcaaagta cgcaattgaa
     3241 cgttgatatt gacggaatca aaaatgatgg ttatacgcac gatcaatatc ctacaggtgt
     3301 aacgaaatac aacaaggcag attatggtga tgttgagttt acgttaggaa atattacgga
     3361 tcaagtatta ccgactgaag attctgatct aaccaacgcg aaagtggacg aaatcgttaa
     3421 agatgtagaa gacaagggat ctaacagtga gtacgttaag aatgcaacca ataaaattac
     3481 ttcagctgta gatgaaaatg gtgagatcac ttttgctgat caaccagcct atgtcaattc
     3541 caaagggaat gtatatgtgg tttatgaaag taaatccgct gctggattag tcacacaaaa
     3601 agcaaaacca atggttgtga ttgcgccaat gacagacaac acaggatcag gatttttaaa
     3661 agatattcat ttgtatccaa agaacattgt ttctaagttg agttttgaat taacgaaatt
     3721 tggtgacgat ggcacagctc aaagcaaaca aacaccttta aaaggggcaa aatttgagtt
     3781 atacaagggc gaacctggaa aaggaacaaa attaggagag cttgtatcgg atgatcaagg
     3841 aaagctgact gcaactgatt tgacgttagg aaaatactac tttgtggaag ttccttcaga
     3901 agttgtagtt ggatcagatc aagaaccaac agcagaccaa tatttgttag gcgcagacgc
     3961 tcgcaacgat gcgcacaata agttaacctt tgaaattacg aatgacggcg tgacaagtga
     4021 tttaaaagct tcctacgtga actataaagc accagtgatc gataaaacag tgacaaatgg
     4081 aacaggagaa gaacattctt tccaaattgg tgacgcagtg aattatcaag gtacaattca
     4141 tatccctaca gatatcgcag gtggaacaga tgggattaca gtaaatggtg taaaaagtga
     4201 aacttctcct tacagtgtat ttaaatgggg cgacacagca ggtaaaggac tttcttatgt
     4261 tgctgaaaaa gcagatatta aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa
     4321 tactgactat aaaatcgaaa attctgatag tggttttgtg atcgatttta tcgtaaataa
     4381 tggacaagta agtgatacag ttgcgaattt acatggtcaa gatctaaaaa tgacttataa
     4441 catgtttgtg aatgatagtg ctgaaatcgc aaatccatta acaaatagcg tggatttcgt
     4501 ttataacaat aatccattta atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt
     4561 gacttatggc gcaaaattct taaaagtcga tagcggccta tttggaacag gtatcaaagc
     4621 aacaccgctt gaaggcgcag aatttgcagc taagaatgca gaaggtaagt actacggtgg
     4681 acttactgat acagataaag atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa
     4741 tgcagcaatc ctaaaatcag ataaagaggg tcattttgaa atcactggtt taactgaagg
     4801 agattactca ctggaagaaa caaaagctcc tgaaaactat caaaaattaa caaaagagat
     4861 ctctttcaaa gtagacaaag attcatacaa agaagaaaac cgaatcacga tcaaaaataa
     4921 tcaaaaagct tccgtaccaa tgactggtag caacggtttc caatcttacg tattgatctc
     4981 ttgcttgttg cttggggcag gcgcaatttc tgcagttgtt tactttaaga agaaagccta
     5041 gaaggatcag ctaatgatta tcagacaccc taagaaaaag cgaatcatgg gtaaatggat
     5101 cattgctttc tggttacttt cagctgttgg tgtcttactt ttgatgcctg ctgaagcgag
     5161 cgtggcgaaa taccaacaaa atcagcagat tgccgcaatc gatcgaacag gaacggcagc
     5221 agaaacggat tcttctttgg atgttgctaa gattgagtta ggcgatcctg ttggtattct
     5281 tacaattcca agtatttctt taaaactacc aatttatgac ggaacttccg acaaaatttt
     5341 agagaatggt gtagggatta cggaaggaac tggcgatatt acaggaggaa acggtaaaaa
     5401 tcctttaatc gcagggcaca gtggacttta taaagataat cttttcgatg atttgccttc
     5461 agttaaaaag ggagaaaagt tttatatcaa agttgatggt gagcagcacg cttatcaaat
     5521 cgatcggatt gaggaagtgc aaaaagacga actacaaaga aactttgtca cttacttaga
     5581 accaaatccc aatgaagatc gagtaacgct aatgacatgt acgcctaagg ggatcaacac
     5641 acaccgtttc ctagtgtatg ggaagcgagt aacctttaca aaatcagaat taaaagatga
     5701 agaaaataag aaacaaaaat tatcttggaa atggctttta ggaagtactg tttttctttc
     5761 agtcatgatc attggttcgc tatttgttta taaaaagaaa aaataaagga gggtaaccaa
     5821 catgagaaat agaaaatggc tatttgtttg tgcagccgct cttttttctt tcactgccta
     5881 cacacagttg acgtttgcag acagacaaga aagcacggat agcacagaac aaacaatcca
     5941 aacgtcagat agcagcacga ttcaaagcag cgatgaaacc gtcgattcgt caacagttga
     6001 ttcttcagaa gaacctaaaa ttgctgaaga cgatcaaata gtcaaacaga ccattcacgt
     6061 tcaaaaaatt atttcttccg aacaattaga caaccagggg caaaagcttg aaaaacaaga
     6121 aggagtaaat ggcgcgaagt tttctgttta tgatgtatcg gacattttac aaaagatgga
     6181 tgttaaggat ctcactactg accaaattga atcccaatta aaagatcgag tgaaaaaact
     6241 atccagtgat caactgaagc taatttctaa tggagaaaca aagacaattg atcaacaaac
     6301 aggtgtcttt gaattctcgg tagaagtaca ggcaaatcag aaacaagctt attatattgt
     6361 caatgaatca agtcctgaaa acatttctaa tagcgaggat attttgctat taacacctgt
     6421 ttctgataaa aatggtgaat tcttaaagga tgtgtggatt tatcctaaat cagaagcttc
     6481 gcaacctaaa gaagaagtaa aaaaaatcgt atctactggc gtaaagaaaa acttctttga
     6541 gaattgttgg gattttgtta ctcacttgtt ttctagagat taaggaagaa ataaaatgaa
     6601 actgatcaaa ttattattat tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg
     6661 tttattattt tctaaactat ctattttgcc aagctaccaa ctatacctaa tgaggatttt
     6721 aaacatacca gctgcttcca tgccgctaga tattatggaa aagctcatta ggaatccatt
     6781 atttactacg atcaatttga tcgttattgt cggaatttca ctagtcattt ataaaaaaga
     6841 ggggatgaag ggatgaattt taaacactcg tggacaagac ggttaaaata tgtttttccc
     6901 ataatgatga tgttaggtac attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa
     6961 gttattgtcg atccgtcaaa tccaatccag aacgtaaaga ataattttat tattccaaaa
     7021 gatgttccta atgcaaaaac aaatattact gtaaacggag cgtcaaaagt ttacaaatat
     7081 catgttgatg ccaacaaagg tggttttaca gacgactacg taggattagt ggagggaaac
     7141 aaaaatatac gctatccttc tttccaaaaa gaatacggtg aaacaaacct tgttttagaa
     7201 ggcgtaacaa ccaacaccaa agtcaatatt gactatggaa aaatcggtac gtataacggg
     7261 aaaaaagtca atatcaagct agttctatct aatattcact tatactcgga tacgttgccg
     7321 tggaatctac tagataacaa ctatacaaaa actcattttc gagacgatgg ctataaaaat
     7381 acgaatggtg caatgagtaa atctaaaaaa agaacagtac tttggatctc agacaatctt
     7441 ttctcaggaa ttgtttacca ttcaacacaa atgaatgtgc agctagtagc cacatatgag
     7501 gacggtagcc ccgttcaatt ttcaggagat acatttatca gttttaactc tttgaaccct
     7561 gcaggaggaa aaagcacgga tcttaaagga gaatatgccc actatgacaa aatgaatacg
     7621 acagattggt atgtcagaaa agatacggtt ctttcagaat ttaaatcttt ctacaacaat
     7681 ctgaacgtgg taggtggcca cccaggagga agctctaagc tgacacaagc ggataatgat
     7741 ttcaataatt tgcatgataa attaggagat ccgaagtttg gtcaaggtac tgtgtccttt
     7801 aaaatatctg aagcaaatcc gacgtttgtg attggttctt ctaatgttca aacctggttt
     7861 actttatctt ctgcgacaat ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt
     7921 gataaaaatg gaaataacgt gaacgataaa atgttgcaag taggagacac gattcaatat
     7981 cgaatcaaac agaaggtgaa tcgattagga gtggatttac tagcggttta cgatcgattt
     8041 gagttaatcg ataatttacc aaaagaagta aattacgtcg atgcacacgt tgaaagtggt
     8101 acaaataaaa aattcgatgt atctggtgaa gtgacctatg acaaaacaaa acaccaggta
     8161 aaatatgcag cgaaagctga tacattaaaa aatcgaatga agtataacgg cgaaacttac
     8221 gaattagtga tcaatgtaaa agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat
     8281 cagggaacgt cgatcattaa taaagtagaa aaggatacaa atatcattac cgtttatttc
     8341 cctaaaattc cagtgaaaga agtacaacaa aatggaaaag atgtaaacgg acgaaatgac
     8401 ggtaagaagg gtacgccgac agctccttta aacgcaggat ctgaagtaca atatctagtc
     8461 acacaaaaat ggcacacaaa aggcgtagac gctgcgtcag atcactacaa acaatttagt
     8521 attcaagatc caattgaagc tcgtttgaca tataaagaag gttctgcgca agtaatagat
     8581 aagagtaaag gaaaagatat tacttctgaa ggcacactaa cctatgatag caattcaaga
     8641 acgctgaagt gggaagcttc cgctgacttc ttaaataaca atcttttaga tggacgagaa
     8701 attcagctga tatttacagc taaaactcca ttacaatcag aaaaaaatat tgataaccaa
     8761 gccgtagtag cagtagataa tgtttctaat aaaacaaatg ttgtgacaat tggggttgat
     8821 cctaacttgc cacaagttat tgttcctaaa acaggttcta cacatttagt aacgatttta
     8881 gcggtcagct tactattact tgtgttagca acttttggct acgcagttct gaagttcaat
     8941 taaacaaaaa aacaaggctg aaaatcagcc ttgtttttct atttgaaaat gggattggca
     9001 ctaataaata cgttgtctga agaaagtgat ccgtctgcag aaacttcata agcatgggat
     9061 aaaccaacga tactttgatt gttgtaaagc aagtttgcgt tatgagcagc gctatctttc
     9121 cacataacca tagcatttac tgctcctgta tctgaaagga tctcgtcaac gtaaaggtag
     9181 aaactattga tattttctcc tttagcaccg tagcagccta agctttctaa gagatcttgg
     9241 aaagtaccgc cgccaggtcg gtaatgactg aagcttactt gtaattcttt tgcacgaata
     9301 tctgcagctt gttgaacgat atcattgaat tgcattgggg caacgccatt ttgttgacgg
     9361 tagctattta accaagcaag ttctttttga cgcacatagt tgtttaaatc acttagtgag
     9421 atatttttgt atttgaaagt gatcgttcga ttttggtcaa cattattgat tacacgttgt
     9481 ccatttttat caacggtaac agatgaatcg tcatttctac acaagaagta gttttgatcg
     9541 gtcatatttt tccaagaagt agagaaacta tctccctcgt aaaccgtttg tgtttcagta
     9601 gataaaacag cgtttgtgtc aacgtttata tggttcacag tgacgttgta ttgatgagta
     9661 taggcaaaat tgattccttg attacttgta acgttgttta aagtcacagt gttgctagag
     9721 ctttctctag gatcaagaac aaaattagtg aacataggtg cttcaattgt gatgctttgt
     9781 ccataaacga cgggataact cttactgtaa ttatagagtt cttggatatt tggatcagta
     9841 tccgagactt tatttggacg agctttcact tcaactagaa agacttcatc atagtaaaaa
     9901 gtaaatgtgg tatttgtgtt aatcgtttgt gtttgtgttt cattcccatt tacttgatac
     9961 tgtggtgctt caaagttagt caagtcggtt ctagcttttg cagtgtaact tgtaccagct
    10021 tttactgaga catgttcttc ttttagcagc gttccagttt gtcgattcat atgacggaca
    10081 gttaccgcat attctgaagg tttaggtttg tgttgtaacc ctttaaatgc agaatcaaga
    10141 ctatttttag cattatttac ttgggtttga gaagctttag aatcgtctag tactttttta
    10201 gcattggata aagcagtctg aaaattattc caagatcctt ctgtaaaatc tcctttagca
    10261 gttgatttta cttggttgta caaggcttgt aattccgttt tgttcacagt aggagctggt
    10321 tttttcttta atcccttgta agcagaatct aatgtgtttt tggcactatc cacttgtttt
    10381 tgagtcgctt ttgagtcatt taacactttt ttagcattgt ttaaagcggt ttggaatgcg
    10441 ttccaggtat tgtcggtata gtcacctttt ttagttccct taacgctcgt ataaagagct
    10501 tctaaagctg atttattcac tttgggttta gaggtgtcag caccatacca accaatacct
    10561 tcgtatttcc agccatagtc tttggttaac cattgtactt ctttccaact tgcagtgtag
    10621 tgatgcgaac cagaggtaac acctggaagg aaaaggcggt agatcggttt attatttttt
    10681 tcggcagaac gccaggcagg cccttcataa gtccaaccgt aatttttggt caaccatttc
    10741 acttcatccc aactagctgt gtaatggtga tcacgaaggc ctttattgta taaacgatag
    10801 acaaggtggc caccgctagt cggtgcttcc caagcggggc cttcataatt accccactta
    10861 attttcacta ggtgatcacg ttcttttgtg ctagaggtgt aaaaatgttc atggttattt
    10921 ggattataaa ggcgatacat gacgttagtt gaagcagcgt cagcagatcc agcaagagca
    10981 actgtggcag caaatgttcc aatagctata acagtttttg ggtttaataa tttcatgttt
    11041 tcaactccct attaaatgat taaatatttt gtttacattc ataatatacc acggtatacc
    11101 gaggtagtca acggtatacc gtggaaattt tgaaataaaa aagccgccct atgaagagtg
    11161 taaggatggc tgtttatatg ctatgaagaa atggattcgg cgtatttggt tagtttggca
    11221 gcattatcaa atcgcatttt ttcaatcgca ctttttttct ttattaaatc cgcaattgtt
    11281 gattctgcga ctgaagaatg tttgctaatt tcatatgctg tcttgccagt ttcaaatagt
    11341 aaccattcga ttttctcttt atcagcgtac attgcaatgc tccctcttga ttttttcttt
    11401 attatagctc ttttttgtaa taaatcacaa tatcaagagg aaaagtttct atagaaaggt
    11461 gggaaattat gagtatccgt tccccgtgta tcaaaatgag gtttgttgct actttcgtta
    11521 tttaatatac taaaataaaa caacgaaaga aggaatagaa tgaaaaagtt aataatggta
    11581 tttacttcgt tgattgccat gattgttatt ttaggtggat gtggtagtga aaaaaataag
    11641 caagatagtc aagataatgg cgtgttaaaa ccaggaacag tgtggaaaga agaagtaggc
    11701 ggacttgttc ataatttaaa aattatagat gatacaacat gggaatattc cgaaagtgtt
    11761 tggcatcctg aacaagtaca aataacagtt gagaagctaa aagactataa aggattagaa
    11821 agatataagg taatcgattc aggtggagta gatttattca taggtaaaac agccttaata
    11881 gttccctata acaaagacgg tttagaaacg tttaaattta ttccaacaaa cgaaaaagaa
    11941 catcgagata aaagtgaaat attaaaagaa tacagtacta aatctggata caagttacaa
    12001 aagactagcg agtgattgct agtcttttta tttatcctga aggaggaatc gcagtggcaa
    12061 aagctaaaac gccatttgaa aaagccgcgt ctaaaatctt agaacgtcgc ggtgtatctg
    12121 aaacacagtg gaaacaagaa gtccttgaaa aagcacaatt tgacctatta cggggaaaag
    12181 acaaggactt agaaggatat atctatcaac gtgaacgtga aaagcttgtt ttcgatgaaa
    12241 ttattaaaca ctaaaaggag cgaacaattt tatgtttgaa tcaattacaa tgaatttttt
    12301 aacacaaccg aaagtagtag ctgcggttga tatttttaga gcattacaag gtgcagcaac
    12361 tagtttaaaa aacaatctga tgatttttgc ggctgtagct gccgttgcct ccattgttct
    12421 tggggggatt ttcttcttct ttggccgaag aggagctgaa aacgggaaga tgcacgtagg
    12481 ttatgtattg atgggggtag gagttatatt agctgccgcc tctattgtgg tttcgttatt
    12541 tggaatgttt ggacaaaccc cacagggact ataaaaaata aatagaagga gtaaaaagta
    12601 cgtggctact ttttactcct tttttatgaa aggagaatac tcatgtactt tacgatttca
    12661 ggcaatacca aaaaagataa agagttaata aaaaaagcaa cgaagaaagt gggaaagcgt
    12721 actgcctttt taccgaaaac aattaaatat tctgtcattg aaacggaaga acagctacat
    12781 gtgttaggac aagtgttaga acaagagaat caatcttctg ttaaattgac tctcacgtta
    12841 catgataaag aaggaaaaat agaaaaggct ggcacagaat acgtttttgg cccactggat
    12901 tatcaaattt cagaaggttt ttatcctaca ttgatggatt tgatccgcct tgatgtgttt
    12961 aaggaaacgg atgaaaccat gagtgtggaa gagtcttatc gaaacgaaaa actgctacaa
    13021 gttttagaag aagagttaga gagcgaccaa agaaaagtga gtcaagtgag tgaagtcgaa
    13081 gtaaatgatg aagtaccatg ggaacgacca atctttccaa agaaggagtc aaaagaaaaa
    13141 gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat atttgaggaa
    13201 gagaacgatg taatgggtca attggaggta gatccctttg aagaaacaat ggaggatgaa
    13261 gagacggccg aagtagaagt tgatctagtt gaagctaatg aagagccaac atggactgaa
    13321 ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagagaa aaacgaaaca
    13381 gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc agagcatgaa
    13441 tggatgaaag aaaaaaataa attattttta aatgagcttt attcgaaata tcaattaccc
    13501 caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg taaaggcaca
    13561 cttttagaac agttagagtc tgttcaatca acaaattggc atgagcttgc acaagaagat
    13621 ttattagcca cttttgaaga acgtcaacaa gaaacagatc aagaaataaa tgagtatgcg
    13681 acggaacagt catctaattg ggagagaaca aaagaggaac taaataaaga agaacaacga
    13741 gtgattgaag aagaagtaca aaaaattaaa gagcgatacg aaaagaaacg cgacaatgcg
    13801 tattccttgt gtatagagaa aattaataac tttactaaaa ctaatcaaga agctttatcg
    13861 aaagaaaaag aaaacttgtt aaaagaacgt ataaaagaac gaaaagaaga gttctaccga
    13921 ggtttacgta cagataagct aaagattagc aaagaattaa atgaacaact gaataaactt
    13981 tatcaaacaa cgactgaagt tcttatggaa agacacgggc agatccagca agaattacaa
    14041 gcacaaattc caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa
    14101 gaagaaaaac aacgacaaga agaaaaagag cgcgaacaat tagaactaaa aaaacgtgag
    14161 cagcatcttg cagaacaaaa agtggcgata gaaaaacaac ggttagagca agaaaaggaa
    14221 aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg acaagcacag
    14281 ttgaattatc tagcacaacc tcaaccagta gctgctgttc aaacgccagt gaacaaccaa
    14341 gcaaatttaa ctccttcacc acagcgttcc tttaacggat ggatggtggg atgtattgca
    14401 agtttaagct tgcttctagg tggaggaaca gtatttgctt ttaatcatat caatcaatct
    14461 tcacaggcag attctgtagc cacaacggcg tcaatcaagg aagaagccca ggaacaagca
    14521 agaaaagagt atgaacaaca attggaagaa ttattgaatc gtaccaatca gtcaacagca
    14581 gagtcaagta tggaaaatag caaagagagt gatcctagta aggctaaaga aggatcaagc
    14641 gattcaactg tacactcaaa cacttccgaa agttcagaat caaatgaggt gaaataatgc
    14701 gtattagaga aacaacgaga cgaaaagaca aacgattcaa tttagaaaag aataggtatc
    14761 tttctcattt ctatcgctat actccttcag gagaagagct accagaaaaa catgacaaac
    14821 gtatttctca aaagattttt tatagtggct tagcttatct tcctttaaac tatgttgtta
    14881 caagaatacc agaagcattt catcgatttg agattattcc aggaagttta caaggagtga
    14941 atatttttca gttacaagaa atgccgcagt tattccattt tgattcaact ggtttttgga
    15001 atcctttgac cccaacaaca agtttaggca cacgtttagt tgtttctacg atttgtgttg
    15061 ttccagctgt acttatggga ttgaaaatgg acatgtggtt tcgtccgcta gcagatgggc
    15121 aaaaaggaga taaccgatta aaaacgttaa aggaaatcca acaaatttat ccgcaaatac
    15181 cagaatcaaa gggaagtttt cctggttatg gcggaatacc aattactcat tataaaaaat
    15241 actggtatat tcagacggat acagtaaata cttgtattgt tggtacttcc cgctcaggga
    15301 aaggccagat agaagtttta gcaaccattg acaatttgtc gcgtgcagaa agacaaagtt
    15361 caatggttgt taatgatcca aaaacagaat tatatgttgc tagtaaagat gaactagaag
    15421 ctagagggta tgatgtgtat gcctttaata tcaccgatcc actgcaatca atgagtgata
    15481 atccattggc actcattgtg aaatactgga agcgaggaga catagacacc gcaacacagt
    15541 taaccaatac ttttacgaat acgatttatt atgatgcaaa tgcttcagac aataaatact
    15601 ttaacgaaaa tgcgcagaaa gctgtcaatg cgcttatttt tgcgttattg gaacaagcgg
    15661 atcaaacagg ggattactcc aaattaacac caaataatct cgttgaacta ctttcagaaa
    15721 ttggcggttt taattattat ccagatcctg agaatgaatt caagcagctc aatgccttag
    15781 atgagttcat gaatcagtta ccccctggaa atatagcaaa aaaacaatat ggggcaacaa
    15841 aaattggatc tgataaagcg aaagggaata tcttgtctac ggcaattaca ggattgaatc
    15901 catttaccct aacgaaaatt gcgaagatga cttctcaatc gaccattgat tacaagtcca
    15961 ttggttttcc gaagtatttg gatctcaaat tacatgaaag cctattaaat caacgcattg
    16021 tcgtggaatt ttatcataag ggaaagaaat ttcacgagga acgagtcaaa gttggctatc
    16081 gtggtttttg tgaaatcaac tttgattgtc atttagagca gggagatcat gtttgcttaa
    16141 aatacaatca tcaaggaaaa caatatgttg cttggtatga gttttctcaa aaagtattga
    16201 aagacgaaga gggaaaaatc gtttatcgaa aaaaacgagg agaaactcat ttgccagaac
    16261 tagaaaaaga agccgttcta acattagatg agaagaaaag taacctatta gtagagtcta
    16321 ttcggatgca ctataccgat aaaccaatgg ctatttttat gttaacgcct gattatgatc
    16381 catctaacca tatcattgtt agcatctttc ttagtcagtt gtataaagaa ttatctacgc
    16441 aatgtgttaa gactaaagga gataaatgtc accgtaggat tcattttatt ttagatgaat
    16501 ttggaaatat gccaccgatg gacaacatgg aaggaatcat gacggttacg gcaggtcgta
    16561 atatgttatg ggatctgttt attcaatctt atcagcaact ttatgcgaag tatggagaac
    16621 aaaacgcagc gattatcaaa gaaaattgtc agacacatat ttatattatg tcgacagatg
    16681 ataataccat cgaagaattt tctaaaaaag tggggaataa gacggttgaa caagagaaca
    16741 gtaccatcaa ttcattaggt atgaatactc atatcaatcg caatgttgat tcagatcgta
    16801 tcttaactcc tgaaagaatt tctacttttt tagaaggtga aacgattgta ctcgctcctt
    16861 taaaacgaca agatttgaaa ggtcataaaa tacggccgta tcctattttt aatcatgggg
    16921 aaacgaatct tccttatgct tatcaatata ttgaagattt caatccatcg tgggatatca
    16981 atgatataga catacattgt acacatgcgg acttggatct taaggcttta caaattgatt
    17041 atgattattt cctaccagat gaatatgcta gaaatctcta tcatggactg catgatcaat
    17101 tagaagaaaa aaacgaagaa aatatgtttt ataaaatcgt tcaatctttt gagaatgaga
    17161 cggctaaaaa attgttaatt caattttacg aagaacaagc aacggatcgt cttcaagaat
    17221 atgttgaaaa gttgatcaaa aatgaagaaa tttctgaaat gattaggaaa cggctgttat
    17281 tggaagctga aaaggcggtg aaagaatgat cattggagga attttctctg gcatagccga
    17341 ctggtggtca ggtttattcg gagaagggga tgcagatccc aaaaccgtaa cgaaagtctt
    17401 acaagattat ggtaattatc tgcagtatca agatgtattc agttggattt gtaatacttt
    17461 tttatgggga ttgatcaaag ggttatataa tctcaatact ttattagaaa aaacaattta
    17521 tcaaagcttt gatttaaaag acttattgaa cgccgctggt gttaatgaac tatttcaaag
    17581 tttgattagt aaggtattgg ctcttttttg tgtcgtcaca ttaatttatt taggcttgaa
    17641 attttttatg agtaaacacc caccgaaagt caaaaatatc ttggttaatt tatttatggc
    17701 catgatcttg atttttggtg gtagttcttt aatcgatgaa gggttaaata tcagtaaaaa
    17761 cttttatgga gacgtcactg cagcaaataa agcagataaa acgggttcac ctgcttttca
    17821 aattattcaa aataatgtga ttgatgtcag tacattacta gcgactgacc cgaataaagt
    17881 agaatcctta ccaacagata aaagaaatgc tttaacagcg aaaaatttca aaacagcgaa
    17941 tatcaatggg attattacac ctgatcaagc taagaaaatg tctgaatcaa gtgatattag
    18001 tacagtacaa aaagatcggt taaaggcatt acagtatcga ctagagttag acgataatgg
    18061 gaaagaaatc cctgttcaaa ttagtgatga aggtttagca aaatatgtgt atacgtcagg
    18121 ctatcgtcga tacattagta gtccagggat tattttggct ggggaatctt ctttgtcggt
    18181 agcctattta tttattttgt ttacaatcgt tacttgcatt attgagctag tatttaaaaa
    18241 attctattta gtaattgccg cgtcaacgga ctttgagaca ggacaacgga tgaaaagtgc
    18301 gatagaagat atttctcaat ccttcttgtt attggcgttt acgattttag aattacgaat
    18361 ctatacatta atgctttcag gtatcggtga tttgcatgcg gtaggaaaga tcaatggatt
    18421 cttgtatgtt gttgctttaa ttgtgctaac tattgcgcta ttcaaaggct ctcaatcggt
    18481 tacgaagatt ttcggtgttg atacgagtct gaaaaacggc agcaattctc ttcttagcat
    18541 gtttgcctta ggaaatattg cgaaaaatgt gggtagcgga gctaaaaacc ttgcaagtgc
    18601 tggtaagagt ggtttaagcc atttaaatga tattcgtaag aatggatttg gccatcgaaa
    18661 agatattacc catgataacg gggaagaaca atcagacgat aaagctacaa ctgtacctaa
    18721 tagtaagaag acagtttccg aaatgttcgg gaaagcgaaa aatggtgttt ctaaagccgc
    18781 agaaggtctt ggttacgtca atgaacgtgg gttaaaagga acagttgaag atcttacaga
    18841 aaaagccaaa gacaccttag aagagaaatt agacggcacg agagctaaag aatttgctga
    18901 agctgtgaag aatccatccg aagcactaga aaaacaaaaa gaaaaagctt cagcagtcaa
    18961 agaaggacta aaagatgcgt atggtgaagg acaagtcaat gcagcagtga aggcaaatac
    19021 aacaagtgaa aaagaaaatc aagaaggtgc agcgattcct tcagccaaag atcaccaaga
    19081 tgagttacat tctaacgaaa attcgatcgg agaaaaagat tcagaagaag gacaacatgg
    19141 tcagttacca aaaaccgatc cagaacaata tcgttataaa tctaacatta attctgaaaa
    19201 cgaaaaacgc atttcagatt taccaaaacg aaatcaagaa gtacaaacgg atgaggattc
    19261 catcgatctg ccaaatgata atggcatcga agttggaact gaaaaatcaa cgaaaattgg
    19321 tttgcctaag acacaaaatt taccaatgaa agatagtcgt ttgaatcaat tcaaagagaa
    19381 tgatcaaagc tttgttcatc aatcacctgc aggtactgtt caaatgaaat caagtcgttt
    19441 gcctgaaaat ggaaatctga caactgtttc ttacaattta gctgaaagtg gtgaaacaga
    19501 gaatgcgtcc gttcaaaata ttgtgacaaa agaagctgca cctaagccaa tcaataaagt
    19561 tcataatgga tacagaccag caaacgtagc atttactgca ccgacaaaaa taaatgtgac
    19621 aagagaaact aaaaacagga acaatgaatt gaaagaacaa ctattgaagg atatcaaacc
    19681 ataaaaggcc tatttaaggg cctttttctt tataaaacaa gtttaaaatg gaaaggagaa
    19741 aattgtggag gatattttaa tagctacgtt tataactgca ctgggagcat ttttaacgat
    19801 atttgtggtg actattaaag gatttaaaga tacggatagt ttacaaaaag atcatcagtc
    19861 tattttagat ggtcaaaaaa cacataccga aagcattatt gtacaccttt cagaaggatt
    19921 cacttatttg aaagatgcta tgcccacatt cacagtaaaa ttaacggaaa tatttcattc
    19981 tgttgagcaa atatcgtcag aattgaaaga gcaaagacaa caaaaagatc gtcaacttgg
    20041 ctcacttaat tttaggcaag aaacgataca agaatcattg gagaatgtaa atcgattaaa
    20101 cgatgaaatg caacgagttc atttggaaaa tgaagaatta cgcgcacaaa attatgattt
    20161 aattcaaaag aaaagaaatc tggaacaaaa aattgttgaa ttaaaagaag aaaatcgtca
    20221 attgaattat caattacgac gaggaaaaaa tcgttctcgt gatttaggaa atgaattaga
    20281 gttgtaatat ttagaaaaac gctgtgagaa atcacagtgt tttttatttc taaaaataca
    20341 aaatataagg ggtatggtta tattatggat aaatacattc atatgaaaaa tcctccaaga
    20401 tttagtgggg aatggaagtt tttcgccttt ttctttttag atctattaac gcttggtggc
    20461 tgcttttttt gggcgaaaat agtcaataat cttgtccact taccactctt ttttgcactc
    20521 ttgaattatg gattatggct tgcgatagcg ttagtgattg tttggcgacc ctataataac
    20581 cctggtcaac gccaaatttt tgcgattatc gaatctattt tctttacgga taagaaccac
    20641 tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga tgaataaatg
    20701 cgagtcaaag gacaaaattc agttgataag aaatccattt tgcagtttgg aaaaccagaa
    20761 gagcgctcta tcttggactt atttccatat aaatcctgta cagaagaggg agtcttaatt
    20821 actagagaag atcattttca gcgttattat cgtgtcgttt ccactgacgt ggagggatta
    20881 aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg tacgtatgtg
    20941 ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca aatcgtgcaa
    21001 aagcggcagt tattagataa aaatcgtatg gaacagtcac taggaagaaa tctacagcga
    21061 ttaagaaaat atgaaaagaa attagtagag gagatccagg aattaaaacg ggcagaacaa
    21121 gaacgtcctg acttaagctt ctttttcgtt attgaaggaa agacaatcaa ggatttacaa
    21181 actagaaatc gtcagttaat gcgatctagt ggcattttag ggttaaaacc actctacaaa
    21241 aaagaattga ttaagattct ttatcgaatg aacaacatga atgacgaata aaaaagatgg
    21301 aggaaatgat tcatgctaga tgtgataaaa acagcttttt caacgcaaag taatcaaaag
    21361 gtgattgatc cattaaaaca acttggtaat cgtgaaatag agattttaaa atcaaaaggt
    21421 tatgatttag atttcttaaa acaagtgatg ccacaaggtg ggttgaattt tgatgaagaa
    21481 tatgtgacct atggaaatgg ttattcacga tgtgtgacag tttatcatta cacaaaaaat
    21541 cctactcttt tttggttggc caatctcatg aacaatcctc atacagtggc cacactagat
    21601 gttagcacag atgaaaagca aaaagtgatt gatcaaatca accgcgcatt agatgaatta
    21661 aatgatcgat cagataatga acgaaaagcg actgaccgaa ataaaagtgc ggaagaatac
    21721 ctggaactag cggaatatgc ccgaaaattg acttctaatg gggaaatttc taaacaagtc
    21781 aaagtgagag tacatgttta cggaaatacg atccaagaag tggatgaacg ggcgcaggaa
    21841 ctgatcgaaa atctaaaagg gatggattat cgtgcggtga cttatctgca taaaaccaaa
    21901 cgagattggc aatcgatgtt taccatgttc gatgaacaag ctgaatggtt tggaaatatc
    21961 aaagggcaat cacttccatc gttaaacatt ggcggaggat tcccgttcca ccaccaaacg
    22021 ctaaaagatc caggaggcac acaatatggg gaaaccatga caggcggccc atttgtctgg
    22081 aatgccacgt ataaaaatag tgtacgttta tctaccaata ccttgcttct aggaatgatg
    22141 ggagctggta aatcgaccgt tcttaaaatg atcgcggaag cccatttagc tgcaggagac
    22201 tttatttggg gctttgaaaa gggaaaagac tttattcctt tcttaaagga atataacggt
    22261 attatggtgc gtttagacgg atctgacggg atgatcaatc ctttagagat ctttgccaca
    22321 cggacgtatg atgaagcatc cagtttatat gacgatggtt cagtcaaaga tttgaagatc
    22381 aatgaagcag cgtcctatca aacgcactta gacaaagtag tctatcaggt acagttagtg
    22441 agtcctcagc taaaaggaac gatgaaagca gaattcaaga cctacttgaa tcgtttttat
    22501 gaagagtatg gaacagtacc acgcggtttt acttcctcaa attcgcgttc aaatactgat
    22561 acgcaagtca cagggaaaga tccagaagct tatccgacgt tcaaagaatt tttagccttt
    22621 ttaggacaac tggaactacc aggggcttcc caagagaaaa agaatcggaa agaagaaatg
    22681 gagtctatcg tggaatcctt atgtgagact tatggcatga tttttgatgg tcatagtacg
    22741 attcgacacc ttgaccaaca acagttggtc ttttttgaca ttgactctat tacaggccta
    22801 gatcgagggg tacaacaatg tcagatgtac atggccatga cgttgatttg gaatcaggca
    22861 ttgattcaag gacgaagaca acgacgctta attaaagaaa aacaaatgga agaacaagac
    22921 ttcaaacgct tcttggcgat gattgatgag tgccataatg cgattaatcc agatttcatt
    22981 gaaacggtaa attatgtcac aaacttccaa cgagaaatgc gtaaagtaga agcaatgact
    23041 gttttagcta cacaatcccc acaagaaatg gtgccagaaa atatgtcttc tgataacttc
    23101 tcgaaactga agaaagtctt tgaattttct tctacgaaga tctttatgcg tatggatgaa
    23161 tctattttac gtcatataga aggattagtt ggtaaatcaa tgacgaactc agaattagaa
    23221 tccattcctc aacaaaatca aggagacgct gtgatcaatt ttggctcaaa agaaaccatt
    23281 cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg
    23341 aaaaccaaaa tgaaagttta atcaaacagt atgttaagcg tcgtgcaaag cggcgcttat
    23401 ttttgtgggt gtttggtact tctgcaggtt tgattactat attgatcact gtgtttgtga
    23461 ccctttttct aatacttgcg gcaggttcta ttgataattc tgattcaagt agtggtggcg
    23521 aagcttttac aggagaatat tcggaaggct tacccattta taaagaaatc aaaggaagag
    23581 gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaag tataaattat
    23641 taccaagtgt gatcctttcg caatacggtt atgaatcagc gtttggtacg tcggcgtccg
    23701 caagaaatga tctcaattat tttgggatta cgtggtttga tgggtgtctg tttcctaaag
    23761 gaactgcgcg tggaattggt ggcattgaag gtggctggta catgaagttt cccaactcaa
    23821 aagctgcctt ctcctattac ggctttatgg tagccacaca atcaaatttt aatgcttgtg
    23881 tcggaaataa aagccctggc gcaagcttat tgatcttagg acgaggaggg tatgccgccg
    23941 caggaattac tgaagacagt gcctattata aaaattgtat gtctattatt aatacaaata
    24001 aattgatgga gtacgatgag tttgcgatta aacactggag agaaggcggc ggaactggtg
    24061 gtacgattac aggaagctgg ggcaatccat tcccaggaag tagcttagat aaaaattcgt
    24121 tcagtggagg acaacttttt gggaaaaatc caggaggaga attccgacct aacgggttcc
    24181 atgatggact agactttggt tcagtcgatc atccaggaag tgagattcat gcggttcatg
    24241 gcggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg tttgggtgcg tgtgtgatcg
    24301 tgattaatta cgatggcttg aatatggtgt atcaagaatt tgcaaatagt acaggaaatt
    24361 ctcgagtgaa agtaggcgat caagtgaaag ttggtcaagt aattgggata cgagatacag
    24421 cacacttgca tttaggattt actcgaatgg attggcgtca agcacaagga catgcattca
    24481 ttgatgatgg tacatggatc gatccgttac catttttgaa tagcagcaaa aaatagatca
    24541 ggagaaaaaa tatggaaaat aaaaccttaa aaatcgtctt aacagctgca attagcgtta
    24601 gtttaatcgg caatattgcg ttgttttggc agcttaaaga agaacaagag acaaatacct
    24661 ataaaattga agaagtggct aaaaacgcgt ctgataagct caaaaaaaat tcttctgaag
    24721 cacaggcgaa gattgacaag ttaaatcaaa ccatcaatga gaaaaaccaa gaaatcgatc
    24781 agctgaagca acaagatggc acaaaagcaa aagaggaagt aactgaagca caaagaaaga
    24841 cggcagaaga gttcggtcaa ttggccattg ataaatcatt aagcagaaat gaattaacgg
    24901 aaaaactaaa agatgtcgca acccaagaag tgatcgacaa attaagtcct gtagacgatg
    24961 atcaccaaca tgatgattac ggaagttact cttttacatt aggtgacgtg caaacttacg
    25021 ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt atttgttgat tatacaatcg
    25081 gcaatcctca attcaaagat gtcaaaccac aaaaaataaa aggtggatta actgtcactg
    25141 aaaaacaagt agatggtcaa tggaaagtca cagactttag ttatttcact cgttaggagg
    25201 aagaaaatga gtaagaaaaa aggagcaaga caacgtttta aaaaacgaaa aagttatctt
    25261 agatggctgg ggatctttat gattctcggc tttttgattc ttgctagtcc gatttacaca
    25321 cataaaaagg tagacatacc agatgaagta agcacgaacg catttttaaa cacgaaggaa
    25381 ctgacgatgg tttcaaaaga aaaaggagcg aaccagcaat tagtttttga atttgcgatc
    25441 gacgatgaag atcagagctt attaaaagaa ctagctaatc ttaattatcg tgtagaagta
    25501 aagacagcta aaggggatta tcaagcaata aaagccaaag tgatcaaggt gtctgatgat
    25561 tattttgtag tgaagttagc acatgtacca gaagaatata ttgctatgcg attaactatt
    25621 attcctgaaa aaattgatcc aaaggtggat atgcaagaac cgcaagatct gattttttat
    25681 attcacgaag ataaggtcaa agatcacgtg aaagacgaag attatgaaca acatgcgatc
    25741 aattacaaag tgaaaggata caagaaagaa caaaaagcag cacaaaaaca aattgaatcc
    25801 ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga aactaaaaga tcaattgcct
    25861 tatcaagtcg cagaagatca ggaaaatact gaaaataaga ttaatagtta ccagcaagaa
    25921 attgaaacat cgaaaacgca aatgaaagat caagaagaga gcattcaaaa acttcaagaa
    25981 aaaattgatc gtatgacaaa agaaaatatt tagaagaata ggtggagtaa aagaaatgaa
    26041 gcgagcgaac ttggtcggca aagatacttt cgctgagcag aaagtattga ttaaagccta
    26101 tctcaaagtt gtaaaagcta aagaaatatg tcaagaagtg aattatctaa cacaacaata
    26161 tctgagttga agaatggtag aaagagttta gataccttaa aattcagatc atttgaagct
    26221 ctatatatag ctgctaaaaa atattttaca gctgataata aataaaaaat taaggtgggt
    26281 tctatgtgaa aaaaacgtat caaaagtttt tatggattag ctatatcgtc ctatttatcg
    26341 ttgggtttct cgtcagtgtg tccgtgatcg tcggaacgct ttttcttttg ttctctgacg
    26401 aagatctggt acttaagggg ttaatgagta cgttatcgtg tgtcatgtta ctttatttat
    26461 gtatacagat caacgaaatt atgccccctc attggcaaga aaagaagaaa gaatttaagg
    26521 aagaaatcga aataaaatcc aatgactgga ttgaatacac atggcttcct ctgaaaagaa
    26581 atagcaaacg aaaggaattt tgggcattgc aattatggcc aataggaaaa ctttttattt
    26641 atatggcttc acttatggtt actttaatcg gttatttggt ggtggtctat ggggtggcct
    26701 attacttgtt aagtgagcca aaacagatcg cggcgtcaaa tattttcacg gcagtaatga
    26761 cattaatgtg tgtcatgtgc ttttctattt ttagttactt actcgtccaa tttatcaggc
    26821 aaaatttaac aatgagtaaa cttttcaaaa gtttattgga cttacttatc ttaggcagta
    26881 tgttaacgac tctaacagat cggttcgaac aatctgaaaa aacggtagag gggataaaga
    26941 acttcttaac gacgattgat caatttataa atggatatat gtatttgttt gaagtaggct
    27001 ttattctcac tttgtttggt tgtgtattta taaaactcat taaaagatca atcgaaaaag
    27061 aaatggttat tgatctaatt ccgacgctga gcggctatga gtattacgtt gattttggaa
    27121 agtatgagga gttgatccat acggatatcg ttcacacgag tatccatgaa gtaaccgtta
    27181 agggcatcag atctacttta aaagatggac aggagacgaa aacaatcgac cgaagaaaag
    27241 aggtctacaa tttttataaa gttgaagcaa gtctacgata tggcattggg aaattgaggt
    27301 tagatgggag taatattcaa caaaacaaac catatgtttt aggaaaagtg gtgatttaga
    27361 acttcctatg aaagatgaat aaataaggag aatcttttga aaatgattct tctttacaga
    27421 attgtgctta tagttggcac ggagttaaaa atcttaaaga aggagcagtt attcaaagtg
    27481 aaagaacatc ataatcagtt actatgtatt aaaacgggta cgatgaacat tcaataccga
    27541 acagaatatt tagaattaac gaatcaaact gattttttgt cgattcgaga gcaacataag
    27601 caagaaaaat taatggctac ttatagccaa ttgattgatg agtgggaaca attcaaggaa
    27661 tctaaaacat aaaattccat ttaaacacct tattttttaa agagcataag gaaacgtgta
    27721 cccactttca aaataaagtg ggtagatagg ctgtgcctat cacgtttctg aaaaaaataa
    27781 atgaccccaa aattttcaat ttatgtgtag gtttatgggt agcaaaaata tgaatgtggg
    27841 cagcaaaaag ttcatttgtg ggtagtaaaa ttcaaatgtg tgtagcaatg tgtgtagcta
    27901 cacacattgc tacacacgct ataacatata gtgaaaagga gatatttatg gaagtaaaaa
    27961 taagagatat tgatgaaaaa acgattcaaa gaattgaccg aagatgtatt gaattaagtg
    28021 aacgcaccgg gactaaatgg agccgaaatc gatacctaaa agttttgatt gaaaatgatt
    28081 ttgaccatga acttatggat catagaaaag atcaatttga tcggttgtta gaaaaatttg
    28141 tggatattca aacttataat acgaaaattt tagaagaata tattgcaaca aataatcaat
    28201 taatcgcctt gttaattgaa taataaaaat agtattgtga atttataaaa ggaggaaatc
    28261 aattttggca aaagcaacaa gaaaacatgt atatctttac gatatgcata tcgaagtact
    28321 tgatgaaata atttctactc atgaagatat atctaattat agtgaagcag tcagacattt
    28381 gtgtatgatg tatgatccaa aaatcgaaga aaaaataaat ccaattcaaa ttaaattgaa
    28441 tgcaatggga aaagaaattt ctatattaac cgaaatggtt tcagagtttg cggatcttca
    28501 tttgaaagat acaggaatac taattggtac aaattcaact gtctatcaag aagcaaaaga
    28561 gcgtgtggaa gggaaaataa aagcgaatca aacgaaaaag tattctccta agatcaagaa
    28621 accaataaat gaatctgttc atgccagagt agacccgttt gatccaacaa agtttttaaa
    28681 aagataaaaa gtagaagggg aacatgatga ataaatcgcc tgcagtcatt ctcacttgtc
    28741 aatttacctt gcccaataaa aaatcttttt caacatacat tgattatatg acaagagaaa
    28801 aagcgctgga agaaaaggaa cgacgcacgc cggacgaaga acaggaactg caacgaatca
    28861 agaatgcttt agaggatttt tatattcctg aaggagaaac tttttcggag agtaagacag
    28921 ataaagaatt cagtgaaacg tcgaatgaag ctcaatcgtt gatggaacat ggaatcaatt
    28981 ttgacaagtt agaggaacaa gattttacca aatatctttc ttatatgacc cgtcactatg
    29041 cgttagaaca aaagaaagaa ctgacgcaaa gggaacaaaa agaataccaa cggttgaacc
    29101 aagcaatgaa aaaatatcaa acagagccac ataaaaaagc ttcaggaaat cttcctggag
    29161 ttttttctat ggcttcagac gaagtgaaag gaagtgatct gaaggagatc aaacaaatct
    29221 tccaaagagg gcaacaaaaa ggctcaatca tttatcaaga tgttgtctct cacgacaatt
    29281 cgtatcttga aaaaatgggc ttgtacgatc ccaaaacaga taccttagat gaagaaggat
    29341 taaaagcagc tggaagaaaa atgatggaga cattattcga gaaagaacaa ttgaatgaaa
    29401 caggttactg gatggcaacg attcaccgta acactaagca tatccatatt cattttgcag
    29461 ttgttgaaaa agaaaatacg cgtaaacctt acacggaggt tgaaaatggt gtgagttata
    29521 tcgtaccgaa aggtaagaga agacaagcaa cattagacgc aatgaagacc aattatgtac
    29581 atgagttaga acgtttttct tatgaaaagg atcgaatgat tggtatggag aaacgaaatt
    29641 tattgacgcg gaaaagtgat ttgcgtaaca ccttaacaaa agtggtaaaa gagccagcgc
    29701 gttacgatcg acacgcaatg aacttattaa atgaagtata taaaacccta cctgacaaac
    29761 gttcggaatg gcattatggg gatgaaaaac gaaccaaact ttcaccatac acaagagaga
    29821 aactcgatga gttaaccaaa tatgtgttaa caaaagaacc tgaatatcaa gaatatatac
    29881 agatttcaaa atcattgaaa gaagaagcaa aaagtcttta tggggaaagc aagagagaat
    29941 caaaggatgc agaaaaaaat gctctctttg atttgaagaa gcgcacagga aatgccattc
    30001 taacagaact gaagaaacag gatagtcaaa taaaaccttt aatagaaagt atggatcaga
    30061 tgcaatctat ttacaatgaa cgatcctttc gaacgcttaa acagttacca acgaatgaat
    30121 acaaatatag ttacgaagaa ttcatgaagc gcaggaaaca attccaacaa agagttgtca
    30181 cgcaacggag tttgcgtcaa ttaaatcgaa cgattaatgc ccatgaagaa aaataccacg
    30241 caagtaaagc ctacgaagag caacaacgaa gaattcaaat ggaacaagaa agacagtaaa
    30301 gacagtagga ggaatagcaa tgaaacaata tttagatcaa tggaaagtta tagaaggatc
    30361 attaagagaa gaacgaatcg aacaacttcc cgattgttta gaaaaagagc atttgtttca
    30421 gataagagaa atgttgagaa acgaacagtt tgatccaaat caatttttag tcgttgagta
    30481 ctcagctact ggtgtgtact gttgtaatca tgtgaagggt gaaaaatatt ttatcattca
    30541 agaatatgag ggcaagttag cgccttatta tactacttgg gaaatgaatg aagaggggat
    30601 caataacttc ccgtgtaaaa gcattgaaga gtctatttca ctcacagaat gttagttgtt
    30661 aagtgaataa aagaagtaaa agagtcctga acgtaattgt ccaggactct ttttttcaat
    30721 gaattgtttt ccgccaattc aatgagtggt gcgatttgcg actacattat atcaaaagaa
    30781 agagaaggga ggaagaagaa atggcttatt caacgattgg agttgttgtt gaaaaaagca
    30841 gagacaatct cgtttttgtg acagaaattc aaacgggcag agcttttgtc gtcacagaca
    30901 aagcggcaaa agcgtatcaa agtggcgata ttttaacgtt aaatctgact acgaagacgt
    30961 tcgtcgatgc ggcagaagat tatccatt
//
LOCUS       DACEMK010000023        30476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEMK010000023 DACEMK010000000
VERSION     DACEMK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.023"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW715_000895"
     CDS             147..1016
                     /locus_tag="IW715_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1021515.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW715_000896"
     CDS             1225..2250
                     /locus_tag="IW715_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1021516.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW715_000897"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW715_000897"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1021517.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="IW715_000898"
     CDS             3333..5036
                     /locus_tag="IW715_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1021518.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW715_000899"
     CDS             5038..7071
                     /locus_tag="IW715_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1021519.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW715_000900"
     CDS             7082..7522
                     /locus_tag="IW715_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1021520.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW715_000901"
     CDS             complement(7722..8849)
                     /locus_tag="IW715_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021521.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW715_000902"
     CDS             complement(8852..9529)
                     /locus_tag="IW715_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021522.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW715_000903"
     CDS             9793..11559
                     /locus_tag="IW715_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1021523.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW715_000904"
     CDS             11574..12011
                     /locus_tag="IW715_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1021524.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW715_000905"
     CDS             12024..13175
                     /locus_tag="IW715_000905"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1021525.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="IW715_000906"
     CDS             complement(13237..13935)
                     /locus_tag="IW715_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1021526.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="IW715_000907"
     CDS             complement(13928..15280)
                     /locus_tag="IW715_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1021527.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="IW715_000908"
     CDS             complement(15503..16903)
                     /locus_tag="IW715_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1021528.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="IW715_000909"
     CDS             17064..17801
                     /locus_tag="IW715_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1021529.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="IW715_000910"
     CDS             17926..18405
                     /locus_tag="IW715_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1021530.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="IW715_000911"
     CDS             18402..19169
                     /locus_tag="IW715_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021531.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="IW715_000912"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="IW715_000912"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1021532.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="IW715_000913"
     CDS             complement(20072..20674)
                     /locus_tag="IW715_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1021533.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21249
                     /locus_tag="IW715_000914"
                     /pseudo
     CDS             20780..21249
                     /locus_tag="IW715_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21355..21939
                     /locus_tag="IW715_000915"
     CDS             21355..21939
                     /locus_tag="IW715_000915"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1021534.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="IW715_000916"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="IW715_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1021535.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="IW715_000917"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="IW715_000917"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1021536.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="IW715_000918"
     CDS             23840..24019
                     /locus_tag="IW715_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021537.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="IW715_000919"
     CDS             24041..25066
                     /locus_tag="IW715_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1021538.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /locus_tag="IW715_000920"
     CDS             25241..26485
                     /locus_tag="IW715_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1021539.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="IW715_000921"
     CDS             26638..27585
                     /locus_tag="IW715_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1021540.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="IW715_000922"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="IW715_000922"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1021541.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="IW715_000923"
     CDS             complement(28487..28729)
                     /locus_tag="IW715_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021542.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="IW715_000924"
     tRNA            28850..28922
                     /locus_tag="IW715_000924"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="IW715_000925"
     CDS             complement(29041..29679)
                     /locus_tag="IW715_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1021543.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29802..30434)
                     /locus_tag="IW715_000926"
     CDS             complement(29802..30434)
                     /locus_tag="IW715_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1021544.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10374 a         5357 c         6156 g         8589 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga
    29761 tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt
    29821 aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt
    29881 tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca
    29941 gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa
    30001 cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt
    30061 gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa
    30121 ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg
    30181 ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc
    30241 attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt
    30301 ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca
    30361 aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact
    30421 ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac aaaagc
//
LOCUS       DACEMK010000024        30465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEMK010000024 DACEMK010000000
VERSION     DACEMK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.024"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..554)
                     /locus_tag="IW715_000927"
     CDS             complement(<1..554)
                     /locus_tag="IW715_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1021545.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(680..2608)
                     /locus_tag="IW715_000928"
     CDS             complement(680..2608)
                     /locus_tag="IW715_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1021546.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2914..3630)
                     /locus_tag="IW715_000929"
     CDS             complement(2914..3630)
                     /locus_tag="IW715_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1021547.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3631..5454)
                     /gene="glpO"
                     /locus_tag="IW715_000930"
     CDS             complement(3631..5454)
                     /gene="glpO"
                     /locus_tag="IW715_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1021548.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5468..6964)
                     /gene="glpK"
                     /locus_tag="IW715_000931"
     CDS             complement(5468..6964)
                     /gene="glpK"
                     /locus_tag="IW715_000931"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1021549.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7100..8560)
                     /locus_tag="IW715_000932"
     CDS             complement(7100..8560)
                     /locus_tag="IW715_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021550.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8550..9845)
                     /locus_tag="IW715_000933"
     CDS             complement(8550..9845)
                     /locus_tag="IW715_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1021551.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10177..10854)
                     /locus_tag="IW715_000934"
     CDS             complement(10177..10854)
                     /locus_tag="IW715_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1021552.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10884..11975)
                     /locus_tag="IW715_000935"
     CDS             complement(10884..11975)
                     /locus_tag="IW715_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1021553.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12076..12510)
                     /locus_tag="IW715_000936"
     CDS             complement(12076..12510)
                     /locus_tag="IW715_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1021554.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13094..14551)
                     /gene="ascB"
                     /locus_tag="IW715_000937"
     CDS             complement(13094..14551)
                     /gene="ascB"
                     /locus_tag="IW715_000937"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1021555.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14548..16443)
                     /locus_tag="IW715_000938"
     CDS             complement(14548..16443)
                     /locus_tag="IW715_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1021556.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16501..18276)
                     /locus_tag="IW715_000939"
     CDS             complement(16501..18276)
                     /locus_tag="IW715_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1021557.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18326..19822)
                     /locus_tag="IW715_000940"
     CDS             complement(18326..19822)
                     /locus_tag="IW715_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1021558.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20016..21638)
                     /locus_tag="IW715_000941"
     CDS             complement(20016..21638)
                     /locus_tag="IW715_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1021559.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21966..23279)
                     /locus_tag="IW715_000942"
     CDS             complement(21966..23279)
                     /locus_tag="IW715_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1021560.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23524..23748)
                     /locus_tag="IW715_000943"
     CDS             complement(23524..23748)
                     /locus_tag="IW715_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021561.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23768..24136)
                     /locus_tag="IW715_000944"
     CDS             complement(23768..24136)
                     /locus_tag="IW715_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1021562.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24332..25042)
                     /locus_tag="IW715_000945"
     CDS             complement(24332..25042)
                     /locus_tag="IW715_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021563.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25060..25557)
                     /locus_tag="IW715_000946"
     CDS             complement(25060..25557)
                     /locus_tag="IW715_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1021564.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25594..26046)
                     /locus_tag="IW715_000947"
     CDS             complement(25594..26046)
                     /locus_tag="IW715_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1021565.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26181..26786)
                     /locus_tag="IW715_000948"
     CDS             complement(26181..26786)
                     /locus_tag="IW715_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1021566.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27236..27856)
                     /locus_tag="IW715_000949"
     CDS             complement(27236..27856)
                     /locus_tag="IW715_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1021567.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27858..28727)
                     /locus_tag="IW715_000950"
     CDS             complement(27858..28727)
                     /locus_tag="IW715_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1021568.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28750..29157)
                     /locus_tag="IW715_000951"
     CDS             complement(28750..29157)
                     /locus_tag="IW715_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1021569.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29176..30465)
                     /locus_tag="IW715_000952"
     CDS             complement(29176..30465)
                     /locus_tag="IW715_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1021570.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8631 a         6627 c         5021 g        10186 t
ORIGIN      
        1 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
       61 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
      121 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
      181 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
      241 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
      301 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
      361 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
      421 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
      481 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
      541 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
      601 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
      661 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
      721 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
      781 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
      841 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
      901 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
      961 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
     1021 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
     1081 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
     1141 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
     1201 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
     1261 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
     1321 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
     1381 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
     1441 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
     1501 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
     1561 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
     1621 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
     1681 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
     1741 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
     1801 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
     1861 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
     1921 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
     1981 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
     2041 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
     2101 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
     2161 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
     2221 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
     2281 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
     2341 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
     2401 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
     2461 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
     2521 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
     2581 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
     2641 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
     2701 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
     2761 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
     2821 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
     2881 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
     2941 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
     3001 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
     3061 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
     3121 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
     3181 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
     3241 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
     3301 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
     3361 aatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
     3421 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
     3481 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
     3541 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
     3601 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
     3661 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
     3721 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
     3781 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
     3841 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
     3901 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
     3961 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
     4021 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
     4081 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
     4141 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
     4201 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
     4261 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
     4321 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
     4381 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
     4441 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
     4501 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
     4561 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
     4621 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
     4681 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
     4741 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
     4801 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
     4861 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
     4921 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
     4981 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
     5041 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
     5101 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
     5161 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
     5221 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
     5281 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
     5341 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
     5401 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
     5461 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
     5521 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
     5581 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
     5641 gcgccgttgt ttccaaattt tctgctcgtt ggatcggtac attcaagata tctgtttgga
     5701 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
     5761 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
     5821 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
     5881 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
     5941 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
     6001 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
     6061 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
     6121 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
     6181 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
     6241 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
     6301 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
     6361 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
     6421 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
     6481 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
     6541 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
     6601 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
     6661 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
     6721 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
     6781 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
     6841 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
     6901 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
     6961 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
     7021 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
     7081 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
     7141 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
     7201 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
     7261 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
     7321 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
     7381 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
     7441 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
     7501 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
     7561 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
     7621 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
     7681 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
     7741 cgtctactat cgaaaaagaa acaaaagagc tgtaaaacat cattgtttcg tatacattca
     7801 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
     7861 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
     7921 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
     7981 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
     8041 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
     8101 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
     8161 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
     8221 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
     8281 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
     8341 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
     8401 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
     8461 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
     8521 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
     8581 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
     8641 tgttcaaacg tccatccttc ttttactgca attgtcagtt ggtcaagaag gtggaacatc
     8701 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
     8761 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
     8821 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
     8881 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
     8941 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
     9001 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
     9061 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
     9121 tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc atagttctcc
     9181 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
     9241 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
     9301 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
     9361 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
     9421 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
     9481 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
     9541 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
     9601 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
     9661 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
     9721 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
     9781 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
     9841 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
     9901 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
     9961 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    10021 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    10081 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    10141 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    10201 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    10261 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    10321 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    10381 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    10441 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    10501 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    10561 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    10621 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    10681 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    10741 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    10801 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    10861 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    10921 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    10981 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    11041 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    11101 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    11161 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    11221 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    11281 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    11341 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    11401 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    11461 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    11521 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    11581 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    11641 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    11701 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    11761 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    11821 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    11881 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    11941 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    12001 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    12061 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    12121 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    12181 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    12241 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    12301 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    12361 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    12421 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    12481 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    12541 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    12601 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    12661 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    12721 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    12781 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    12841 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    12901 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    12961 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    13021 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    13081 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    13141 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    13201 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    13261 acgacgtata accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat
    13321 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    13381 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    13441 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    13501 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg
    13561 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    13621 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    13681 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    13741 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    13801 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    13861 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    13921 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    13981 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    14041 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    14101 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    14161 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    14221 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    14281 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    14341 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    14401 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    14461 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    14521 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    14581 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    14641 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    14701 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    14761 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    14821 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta
    14881 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    14941 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    15001 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    15061 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    15121 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    15181 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    15241 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    15301 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    15361 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    15421 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    15481 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    15541 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat
    15601 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    15661 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    15721 aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    15781 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    15841 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    15901 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    15961 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    16021 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    16081 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    16141 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    16201 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    16261 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    16321 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    16381 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    16441 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    16501 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    16561 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    16621 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    16681 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    16741 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    16801 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga
    16861 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    16921 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    16981 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    17041 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    17101 tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt
    17161 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    17221 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    17281 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    17341 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    17401 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    17461 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    17521 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    17581 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    17641 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    17701 tgtactgatt tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga
    17761 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    17821 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    17881 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    17941 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    18001 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    18061 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    18121 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    18181 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    18241 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    18301 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    18361 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    18421 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    18481 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    18541 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    18601 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    18661 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    18721 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    18781 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    18841 aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta
    18901 acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc
    18961 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    19021 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    19081 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    19141 acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt
    19201 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    19261 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    19321 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    19381 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    19441 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    19501 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    19561 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    19621 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    19681 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    19741 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    19801 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    19861 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    19921 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    19981 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    20041 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    20101 ataaccctta aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa
    20161 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    20221 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    20281 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    20341 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    20401 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc
    20461 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    20521 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    20581 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    20641 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    20701 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    20761 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    20821 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    20881 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    20941 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    21001 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca
    21061 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    21121 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    21181 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    21241 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    21301 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    21361 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    21421 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    21481 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    21541 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    21601 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
    21661 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
    21721 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
    21781 ttccctcaac tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac
    21841 ttctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat
    21901 ataagaaact attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa
    21961 cctctttatt tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac
    22021 atgattggag atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata
    22081 aaacctaatg caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag
    22141 aatgaaggga tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc
    22201 aatactccta ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat
    22261 ggagaaaaga acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa
    22321 cgtcctccaa cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca
    22381 aataaggaac ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc
    22441 atcttgttgt ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta
    22501 tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg
    22561 tcccctacat gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca
    22621 atttcagaag gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc
    22681 ccgatgaaga tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca
    22741 aaaatcgtca accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca
    22801 ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg
    22861 ccagaaatcg catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg
    22921 aagattgtga tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc
    22981 catggaatcc ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca
    23041 ggtgccgttc caattggata acgggctaat acccccatta aaacacagcc gatcgcactg
    23101 gctaatgcag tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt
    23161 ggatttacaa ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca
    23221 cgttttaccg tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc
    23281 ttccatcccc ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat
    23341 aaaacaacgt gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt
    23401 taagcaaaat ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt
    23461 cactttggat tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa
    23521 aaatcaatag tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt
    23581 tgtatcgaca ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca
    23641 gccttttgtc ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt
    23701 tgctcgccga tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc
    23761 tttttattca atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag
    23821 aaaaattgaa agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct
    23881 cgtagatagt ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct
    23941 ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga
    24001 tgctgagtac aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac
    24061 caattttttc tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac
    24121 gtgttttttg tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa
    24181 ctgattccat tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat
    24241 aaagcaatag tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc
    24301 aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg
    24361 ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt
    24421 cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg
    24481 ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac
    24541 agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat
    24601 ctgcacctaa aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg
    24661 cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat
    24721 agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta
    24781 aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc
    24841 actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt
    24901 ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat
    24961 cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg
    25021 tatccactat tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa
    25081 tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa
    25141 tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat
    25201 ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat
    25261 accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc
    25321 catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc
    25381 gatactcact aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag
    25441 tgcataagct tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt
    25501 atctgtaaat gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt
    25561 tcgaatttcg tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt
    25621 gacaaaattc caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg
    25681 ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg
    25741 aatgatgaaa ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt
    25801 attttcttta tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg
    25861 ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg
    25921 attttcgcaa atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa
    25981 ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc
    26041 agccatttca tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa
    26101 tgcaactatt ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt
    26161 ttcttctttt acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt
    26221 aactgtttct tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat
    26281 cccatgttgg aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa
    26341 gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca
    26401 aaaactgctc gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta
    26461 cgcacatttt cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct
    26521 ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca
    26581 tctacttttg gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa
    26641 gtttcttttc ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc
    26701 tctttatcaa atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt
    26761 ttgtattgag ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta
    26821 tgtaatcaag tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat
    26881 aaataaaaga atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga
    26941 ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga
    27001 cgagctattc ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc
    27061 cccactaaac ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca
    27121 tgagaagctg tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga
    27181 cctctcggta aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact
    27241 tattgtcatt gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat
    27301 agtctgttac atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg
    27361 cgatgatctt tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca
    27421 atacgtcacc atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat
    27481 cacgtttgta ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca
    27541 cctgatatcc gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag
    27601 gatactcgga cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat
    27661 agaaaggata aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga
    27721 tggattctgc atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc
    27781 gttcccgtag tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa
    27841 tgaaatactt tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac
    27901 agtccaatct tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg
    27961 cgaagagact tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg
    28021 actaaaagct ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa
    28081 tcttcttctt tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg
    28141 atttctttca gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta
    28201 ccgatagcaa tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc
    28261 ggtcttgtat ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt
    28321 gttccaagtt ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt
    28381 gtcttcagga tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat
    28441 ccgctgtcga ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact
    28501 gcatcaacca tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg
    28561 atatatttag cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct
    28621 gcgcctagca tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa
    28681 ttcaactctt ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac
    28741 cttatctttt tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc
    28801 cattttgccc atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg
    28861 cttctggtta tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc
    28921 caaactcttt tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag
    28981 tccaacttgt tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg
    29041 tttcaattgt gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc
    29101 ttcttttgcc agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat
    29161 tttccctact ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag
    29221 tgctgcggct actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa
    29281 gatcgttact agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat
    29341 gatcgctcct cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa
    29401 gaaattggtt agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat
    29461 ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata
    29521 aggaaagaaa actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat
    29581 ccctcgaaat gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata
    29641 cacacccact acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc
    29701 tgataattca gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac
    29761 catcacgacc atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt
    29821 gatatcttcg atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc
    29881 ttcaaataag tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat
    29941 tttacgaatg atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa
    30001 accaccgacc aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt
    30061 catcgttgcc atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa
    30121 acgagccaaa ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc
    30181 taaacccaaa tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg
    30241 catatgaaat ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct
    30301 cgaaccggca ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg
    30361 tcctgctgat ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga
    30421 tgcctgagtc aaaatatttt cgatgataaa attgataacg cccat
//
LOCUS       DACEMK010000025        30351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEMK010000025 DACEMK010000000
VERSION     DACEMK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.025"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            370..1059
                     /locus_tag="IW715_000953"
     CDS             370..1059
                     /locus_tag="IW715_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1021571.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1046..2254
                     /locus_tag="IW715_000954"
     CDS             1046..2254
                     /locus_tag="IW715_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1021572.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2548..3819
                     /gene="serS"
                     /locus_tag="IW715_000955"
     CDS             2548..3819
                     /gene="serS"
                     /locus_tag="IW715_000955"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1021573.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4064..4759)
                     /locus_tag="IW715_000956"
     CDS             complement(4064..4759)
                     /locus_tag="IW715_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1021574.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(4849..5949)
                     /gene="ychF"
                     /locus_tag="IW715_000957"
     CDS             complement(4849..5949)
                     /gene="ychF"
                     /locus_tag="IW715_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1021575.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(5958..6149)
                     /locus_tag="IW715_000958"
     CDS             complement(5958..6149)
                     /locus_tag="IW715_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1021576.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(6170..7060)
                     /locus_tag="IW715_000959"
     CDS             complement(6170..7060)
                     /locus_tag="IW715_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1021577.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(7047..7814)
                     /locus_tag="IW715_000960"
     CDS             complement(7047..7814)
                     /locus_tag="IW715_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1021578.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(7826..8539)
                     /gene="rsmG"
                     /locus_tag="IW715_000961"
     CDS             complement(7826..8539)
                     /gene="rsmG"
                     /locus_tag="IW715_000961"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1021579.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(8771..10672)
                     /gene="mnmG"
                     /locus_tag="IW715_000962"
     CDS             complement(8771..10672)
                     /gene="mnmG"
                     /locus_tag="IW715_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1021580.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(10689..12086)
                     /gene="mnmE"
                     /locus_tag="IW715_000963"
     CDS             complement(10689..12086)
                     /gene="mnmE"
                     /locus_tag="IW715_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1021581.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            12430..12768
                     /locus_tag="IW715_000964"
     CDS             12430..12768
                     /locus_tag="IW715_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021582.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(12936..13694)
                     /locus_tag="IW715_000965"
     CDS             complement(12936..13694)
                     /locus_tag="IW715_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1021583.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(13712..14530)
                     /locus_tag="IW715_000966"
     CDS             complement(13712..14530)
                     /locus_tag="IW715_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1021584.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(14527..14883)
                     /gene="rnpA"
                     /locus_tag="IW715_000967"
     CDS             complement(14527..14883)
                     /gene="rnpA"
                     /locus_tag="IW715_000967"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1021585.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(15001..15135)
                     /gene="rpmH"
                     /locus_tag="IW715_000968"
     CDS             complement(15001..15135)
                     /gene="rpmH"
                     /locus_tag="IW715_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1021586.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            15658..16992
                     /gene="dnaA"
                     /locus_tag="IW715_000969"
     CDS             15658..16992
                     /gene="dnaA"
                     /locus_tag="IW715_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1021587.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            17190..18320
                     /gene="dnaN"
                     /locus_tag="IW715_000970"
     CDS             17190..18320
                     /gene="dnaN"
                     /locus_tag="IW715_000970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1021588.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            18549..18791
                     /gene="yaaA"
                     /locus_tag="IW715_000971"
     CDS             18549..18791
                     /gene="yaaA"
                     /locus_tag="IW715_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1021589.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            18778..19902
                     /gene="recF"
                     /locus_tag="IW715_000972"
     CDS             18778..19902
                     /gene="recF"
                     /locus_tag="IW715_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1021590.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            19899..21845
                     /gene="gyrB"
                     /locus_tag="IW715_000973"
     CDS             19899..21845
                     /gene="gyrB"
                     /locus_tag="IW715_000973"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1021591.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            21865..24336
                     /gene="gyrA"
                     /locus_tag="IW715_000974"
     CDS             21865..24336
                     /gene="gyrA"
                     /locus_tag="IW715_000974"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1021592.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            24572..24871
                     /locus_tag="IW715_000975"
     CDS             24572..24871
                     /locus_tag="IW715_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1021593.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            24921..25433
                     /gene="ssb"
                     /locus_tag="IW715_000976"
     CDS             24921..25433
                     /gene="ssb"
                     /locus_tag="IW715_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1021594.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            25459..25695
                     /gene="rpsR"
                     /locus_tag="IW715_000977"
     CDS             25459..25695
                     /gene="rpsR"
                     /locus_tag="IW715_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1021595.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            25838..27811
                     /locus_tag="IW715_000978"
     CDS             25838..27811
                     /locus_tag="IW715_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1021596.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            27817..28269
                     /gene="rplI"
                     /locus_tag="IW715_000979"
     CDS             27817..28269
                     /gene="rplI"
                     /locus_tag="IW715_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1021597.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            28543..29910
                     /gene="dnaB"
                     /locus_tag="IW715_000980"
     CDS             28543..29910
                     /gene="dnaB"
                     /locus_tag="IW715_000980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1021598.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         9308 a         5895 c         6022 g         9126 t
ORIGIN      
        1 agttatctta tcatgtcatt tccaacatgt caacaactta ttttttatta tttaataagt
       61 tattggtgtt cttttgaaca aataataata tatcaacatc acatgtgaaa gtcaataaga
      121 aaatgcttat ttatcaacat ttctagctta catgatcatt ggttagttct ggtccttttt
      181 cgttcgtctt tataactccg agagacgtaa aatccttttg ttttcagttt aatgttcatg
      241 ttcttgtctt attcacacca actagatgtc tcaccttcta aaattttcct taacaaagtg
      301 ctgcttttat ctatttctgc ttttttttgc tagaataata gtaacaatat gactctggag
      361 gatttattta tgaaaatttt gattgctgac gatgataaag aaatcgttga attattaagt
      421 atctatgttc acaatgaggg gtatgaagca gtaaaggctt atgatgggaa agaagcatta
      481 tctaaaatca gaacaatccc tgatattgaa ttacttattt tagatatcat gatgcctgag
      541 atggatggga tgcaagtagt gaaagagcta cgaaaagaat cccaaatacc aatcattatg
      601 ctgacagcaa aaactactga tatggataag atcaaaggac tggtcgctgg tgcagatgat
      661 tatgtgacca agccatttaa tccgttagaa gtaatggcta gagtaaagtc tttattacgg
      721 cgtacaaaaa tgcagattgc cccagatcag cctgatgtat tagaagtaaa tgcattaatc
      781 atcaataaag actctcatga agttaaaaca atcgatggga aagaaatcca gttaacagct
      841 ttggaattcg gtatcctcta cttgcttgct agtcatccta atcgagtatt tagcgcggat
      901 gaaatttttg agcgtgtttg gaaacaagaa agtattgtct ctgctaaaac tgtcatggtt
      961 cacgttagtc atttaagaga taagattgaa gaagcaactg gtggtgaaaa agtcattcaa
     1021 accgtttggg gagtgggcta taaaattgac gcaagatagt aaacgagcaa ctgaaaaacg
     1081 ccgtcggatc aatttgactt caaaagaaat cagcgaattg ttagcggaag gaatcatcac
     1141 gattatcctt ttgcttctat taaatgtttc tattctggtt gttatcagtt cggtgatcaa
     1201 cagttcacct tctttgacga atgcgatttg ggattcaaag aatatttttg cagaacgatt
     1261 gaacactgat ttgttctgga acggccggaa ttttattatt ccctttttct ttctattaga
     1321 tattggtgtt ttgtattggc gcttgattcg aagatatcgc caaatgcaat tgcggcatat
     1381 catcagcgaa cttcactaca tcgcaaacgg aaattatgat catcgtatcc cttttgaact
     1441 gagtggtgac ttaagccgag tggtaacaag tatcaatgga ttggtggata gtaccgtagc
     1501 agcgattgaa gacgaacgta aaatcgaaaa atcaaaagat gaattgatca cgaatgtcag
     1561 tcatgatatc cgtaccccac tcacgtccat cattggatac ctaggactga tagaagatgg
     1621 ccagtatcac tcagaagaag atcttttaaa gtatacgcat actgcttata tcaaagcaaa
     1681 gcagatgaaa tctttagtgg acgacttatt tgaatacact aaggtacgcc agcctgctgt
     1741 cccggtgaat ttttctgcct ttgacatgat ccagttgatc gaacagttag cagctgattt
     1801 tgagttggaa gcttctaaaa aaaatatcca aatacttgtt caatcaaaag tagactccct
     1861 tatcatggat ggcgataccg aaaaacttgt acgtgtcttc aataatcttt tgacgaatgc
     1921 gttgaaatac ggaaaaggag caacgaagat cgtaatcgaa gtagaacgta tcggttcaga
     1981 agttgttgca acagtcaaaa ataacggagc aatgatccca caacaggcaa tcgataatct
     2041 attcgaccgt ttttatcgtg tagaagaatc tcgttcacaa gcaactggag gaactggact
     2101 tggtttagca atcgctcaaa gcattgtggc tttgcacggc ggatacattt atgcaaaatc
     2161 agataagcaa tggacttctt tcatcatgca ccttccaatc aagaaaaatg aaaaattacc
     2221 tattgataca caggaagtta ttgacgaaag ctgaaatatt cgctattctt attacaatta
     2281 ttagaataaa tgaaaaacac agaacccagt acactttatt ctttttcaga gagctgatgg
     2341 caggtgtaaa tcagcaaagg ataatttgga agttccagtt cgtttacgtg ccctttcgaa
     2401 ataagaaagg tcggttcaac agccgttatg tgttcaacaa gtgcagctga cgctgaactt
     2461 gggtggtacc gcgaatttca gactgattcg tcccaagaat aatgggatgg atcagtcttt
     2521 ttttattttc aaaaaggagg aaatcatatg ttagatgtaa aaatgatgcg acaaaatatt
     2581 gaagaagtac aagaaaaatt gatgactcgg ggcgtaaaaa aagaaacatt agctgaattc
     2641 atggaactag atgaaaatcg tcgtaagcta ctagtcaaaa gtgaagaatt aaaaaaatat
     2701 cgaaatgatg tttctgctga gatcgcccaa ttgaagcgtg caaaagaaga tgcttctgct
     2761 aaaatcgctg aaatgaaaga agtcggtggc aacatcaaag cattggatac tgaaattgct
     2821 gaagtggatg aaaaattgaa acaaattgca actactttgc caaaccttcc ccatgattct
     2881 gtgccagttg gaacagatga agaggacaat gtagaaatcc gtcgttggag cgaaccacgt
     2941 acatttgctt ttgaaccaaa accacactgg gaaattgcag aaaatcttga tattttagat
     3001 tttgaacgtg gcgcaaaagt agccggtagc cgttttgtct attacaaagg attaggtgct
     3061 cgattagaac gtgctgtcta caacttcatg cttgatcagc atatttacga acacggctat
     3121 actgaaatga tcactcctta tatcgtcaac agtaaagcga tgtttggtac gggacaattt
     3181 ccaaaattca aagaagatgt tttccaattg gagaatactg atctaacatt aatcccaaca
     3241 gctgaagtac cattaactaa ctattataat gatgaaattt tagatggtgc agatttacct
     3301 atttatttca ctgcactaag ccctgctttt cgttctgaag ccggaagtgc tggtcgggat
     3361 acacgaggac tgattcgtct tcaccaattc aataaagtag aaatggtcaa attttcggat
     3421 gtagagcatt cttacgaaga attggaaaaa atgacagctg atgcagaaga tatcttgaaa
     3481 aaattgcatt tgccttatcg tgtgatggcc ttatcaactg gagacatggg cttttctgca
     3541 gctaaaactt atgacttaga agtatggata cctgcacaag atacttatcg tgaaatcagt
     3601 tcttgttcta actgtgaaga tttccaagca cgccgcgcaa tgatccgtta tcgcgataat
     3661 gatggaaaaa tccactatac tcatactctg aatggttcag gacttgcagt tggccgtaca
     3721 gtagctgcca ttttggaaaa ctaccaaaat gaagatggtt ctgtcacagt tccagaagcg
     3781 ctcgttcctt atatggggaa tctgaaagtt attaaataaa gaggctgtga aaaagccgct
     3841 tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttt gcataaattg
     3901 gacttaatac cgagaaactg gcttttgaac accgtttatc gtaaaaaggg actgtgacaa
     3961 gacttttgtc acagtccctt actattattt aactatggat aaacaaaatg aggttcttta
     4021 ctacccctag atatctagca aaatagctga tctttttgct agtttatctt cttgggacgg
     4081 ataaacttcc aataatcccg tattttttct tcaagtagta acggattgct aaagcagttg
     4141 ccccgataat gataactact actggatcta atactggatt gataactggt gtcaatagtg
     4201 ttgtcgcaga aaacagtacg acccaaagaa gtgtcgttcc acctaggata agcaatgtct
     4261 tacccattcc tggtttcttg cttcgatctg ctccgggata ttcatattga tagatgtatt
     4321 tgtacatcat ataaaagacc cagccaccga ttgcagaacc tagaacgagt gtagaaaagc
     4381 cataagctgg cgttgttcca cgtgaaaaca atccggtaat cgcaatcatg atacttaata
     4441 gaccaagcaa cagcaacgta ttatctaacc acatcaataa aggggtggat gaatttttta
     4501 cttctgctgg cttattgatg attgcttccg ttctttcaga gactgtccca aacaattgac
     4561 gggctgttct tccgcctttt tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt
     4621 ttactttttc ttcttcagac aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt
     4681 actgttgatt tcttttggtt aattttgttt ctaaactatt gttttctttt acgattgctc
     4741 gtaattgttc tggttccatt atctaatagt cctccttagc cgttcctttt ctcaacggtc
     4801 gtcacaaaag acattctacc atattctaca gctagtttga tagccctttt acacattaaa
     4861 gcggaataac attacgtcgc cgtcttgtac gacataatct tttccttcta atcgaacacg
     4921 tccagcttct tttgctgcat gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt
     4981 ttctgcacgg ataaatccgc gttcgaaatc tgaatggatg attcctgctg cttgtggtgc
     5041 tttgattcct tttttgaatg tccaagcacg tacttcttgt tccccagcag taaagtaagt
     5101 agctaagcct aacaaatcat aagcagcacg gatcaactga tccaaacctg attcttcgat
     5161 tcctaaagct tctaaaaatt ccgctttgtc ttcttcatct aattctgcga tttcttcttc
     5221 tgcacgagca ctgaccacga tcacttctgc attttcttct gcagcaaagt tgcgcacttc
     5281 ttgtacataa tgattcgcgt ctgcatctgc tacgtcatct tctgagacat tggctacata
     5341 aagaattggt tttgtcgtca ataaaaatag tgatttaacg attttttgtt cttcttcagt
     5401 aaattcgatc gttcgggcag attttccttc ttccagtaca ggtttgattt tatccagtac
     5461 agctagttct gccactgcat ctttatcttt ggttttagca acttttgcta cacgagtata
     5521 gcgtttgttg atagattcta agtctgctaa cactagttcc aagttgatcg tatcaatatc
     5581 ttctagtgga tcaacacgtc cttctacgtg agtaatatta tcatcatcaa aacagcgaac
     5641 aacgtgacaa atagcgtcta cttggcggat atggcttaag aattgatttc ccaaaccttc
     5701 tcctttactt gcgcctttta cgattccagc aatatctgta aattcaaaag tcgtcggaac
     5761 agtttttttc ggatgtacaa gttctgttaa gcgttgcaaa cgccagtcag gtacttctac
     5821 catccctacg ttggggtcga tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt
     5881 tgtgatcgca ttaaacaaag ttgattttcc tacgttgggc aacccaacaa ttccagcagt
     5941 caatgccata aatttattca ctctttctct gcattttttt caattacttt ttttaatttc
     6001 ttttcaaaat ctcgacgggt catcatgacg atatgtccgc aattcataca tctgattttg
     6061 atatctgcgc ccattcgaat gacttcccag cgattcgtct ggcaagcgtg aggttttttc
     6121 atttcaacga tatcgtgcaa gtcgtacata aaaatagcct cctttttgtt tattcttcat
     6181 caaattgaat attcaagata tcaagaattc gagtcaaatc tttttgagaa agatattcaa
     6241 tctcgatctt acctttccca tttttttctt ggatagctac acttgtacca aatttatcca
     6301 tcaaacgatc ttcgctttct cggatataat atggtttttc ctttgctagc cttgggattt
     6361 ttttcttttc ttttccatca ttttgattta actcagaaac aagctgttcc agttggcgga
     6421 cagttaaatt ttcagttact gcacgattgg ctaatttcaa gatcaatgtt ttatttttta
     6481 aacctaacag cgtacgggct tgtcccatag ataaacgctg atcttgtacc atttccttga
     6541 caagatccgg taaagaaagc aagcgcaaat aattggcgat ataaggtctg ctctttccta
     6601 aacgactagc tacttctgct tgtgttagtt tcaggttttt catcagcatc tcatatgctt
     6661 ctgcttcttc taacggattc agatcttctc gctgcaagtt ttccagcact gctacttgca
     6721 tcatcgcttc ttcgtcaaag tctctaacga tagcagggat cgtttctttt tcagctagtt
     6781 tggacgctcg aaaacgtcgc tcccctgcaa taatctcata tccttttact gcggaacggc
     6841 gtacgatgat gggttgaaag acacctgatt gttcaataga attagctagt tcctgcaaag
     6901 atacttcttc aaatgtttta cggggctgat acggatttgg tcgcagctca ttcaatggta
     6961 gctggacaac ggtatcatct tttacatcca ccgtctctaa gttttctaaa tcttgaaaca
     7021 gtgcatcgat tcctcggcct aatcctttac ttttcttcac gacttaccac ttcctttgcc
     7081 aatgcttggt agacttcagc tcctcgagaa cgtggatcgt agtcgatgat cggttttcca
     7141 tgactaggtg cttctgataa acgaatattt cttggaatga tcgtttcgta aactttttcg
     7201 cggaagtatt tacgtacttc ttctacgact tcatttccca gatttgttcg tgcatcatac
     7261 attgtcagta agactccctc gatctctaat tctggattaa agtgtttttg gactaaacga
     7321 accgtattca gtaattggct taatccttcc aatgcgtaat actcacactg aacaggtatc
     7381 aaaatcgaat cacttgctgt aaatgaattg attgtcagat ggccaagaga aggcgggcaa
     7441 tcgatcaaaa tataatcata ctgactgctt acttctgcta aagatccttt taatcttgat
     7501 tctcttgcca tcattgacgt caattcgatc tctgctcctg ctaattggag agtcgcaggc
     7561 acaatactca aattttcgtg ttcagtgatc aaagttgcct catcaattgg caattcattg
     7621 actagaacat catagatgtc tcttgtcaca tcaggttttc tgatcccgac accactggta
     7681 gcatttcctt gtgcatccat atccactagc aatacttttt tgccaaggct agcaagacaa
     7741 gcacctagat tcaccgtcgt cgttgttttg ccgacgccac ctttttggtt cgcaacagat
     7801 attatttgtg ccatctcttc tcctcctatt tgattggctg cttattaggc agtccgggct
     7861 ttcttgggta tttctttggg gttggtttct ttttttgaat aacgatgata tgccgttgat
     7921 cagagacatt cggcaaggta accgcctctt ctttgaggaa tttaccacct aataaagcaa
     7981 tcgcttgttt tgcttctgtc aattcctctt cactttttgc tgctttcaat gcataaaaat
     8041 agccttcttg tttagccaag ggcaaacaca attccgctaa cacattcaaa cgagcaaccg
     8101 cacgagcagt gacaaggtca aatgatgcac gaaagtgagg attttgccca aatgtttctg
     8161 cccggtcatg atataaggaa gcatcgattc ctaataatcg ggttaactca gataagaagg
     8221 ttattcgttt attcagagaa tcaacgatcg ttatctgcag ttctggaaag gcaattttta
     8281 aaggtatgct aggaaaccct gcccccgaac caacatcgca taaagataaa ggttgcgtaa
     8341 agtctacttc aaatgctaat gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt
     8401 catccgtaat agctgtaagg ttcatctttt cgttccattc gactaatgtc cgataataaa
     8461 tagcgaattg attcatttga tgatcggtaa gctctatccc tttttctgcc aaggattggc
     8521 gaaattcttc tggtatcata aatattcctt tcattgtgaa acagcaattg tttcacagat
     8581 tctctcccta ctttaacgca aaatctctag acatgcaatc cttccatatg gattcttaaa
     8641 aatttgaaaa gtaagaaaag ccgatagact ttttctttct agtatgactc ataaaatata
     8701 cacagaagag agaactagct ttgagcaatt tctgcaaaaa ctagttctct cttctattta
     8761 ttattacgta ttacgctggt tcactgactt tcgcaatctt cccttgttcg atatagacca
     8821 ttaggatact taggtctgca gggtttacgc cactgattcg gctagcttgg gcaattgttt
     8881 ctggttggat tttctgcaat ttttgttttg cttctgttgc taatccgttg attgctgcgt
     8941 agtcgatgtt ttctggaatc tttttcgctt ccatgcgttt taatttttct actttttcca
     9001 tggctttttt gatgtagcct tcatatttga tttggacttc tacttgttcg atttcttttc
     9061 gtgtcaattc ttcattttct ggaataaacc gtagaagttc tgcataagta atctcaggac
     9121 gtcttaaaaa gtcgcttgct agtacaccat cttttaatgg tgccgcatta atcgttgcta
     9181 aaaagtcttg gacttcttct gttggtttga ttcgtacatt catcaaacgt ttgatttctt
     9241 gttcaactgc ttcttttttc ttcaaataat tcttgtattg atcttctttt actaagccta
     9301 cacggtgacc catttctgtt aatcgcaaat ctgcattatc atgacgtaaa agcagacggt
     9361 attcagcacg tgaagtcagc aaacgataag gttcgttcgt tccttttgtt accaaatcat
     9421 cgatcatgac accgatgtaa ccgtcacttc gtttgatcac taaaggttct tttccctgaa
     9481 ctttcaaagc tgcattgata ccggcaatca gcccttgtcc agccgcttct tcataaccgc
     9541 ttgtaccatt tgtttgtcca gctgtataaa gattttcaac gactttagtt tctaatgtcg
     9601 gacggagctg gtgtggctct actacgtcat attcgatagc atatcctgta cgcatcattt
     9661 ctactttttc taatccagca atagaatgaa gcatgttgac ttgaacatcc tctggtaaag
     9721 aagtagataa accttgaaca tagacttctt ccgtatttaa gccttctggt tccaaaaata
     9781 attgatgacg tggtttgtct gcaaaacgca cgatcttatc ttcgatggat ggacaatagc
     9841 gagcgcctac cccttcaacg atccctgtaa acattggggc acgatgtaag ttgtctcgaa
     9901 taatttggtg agtcgtctca cctgtatagg tcagccagca agattcctga tctagtttat
     9961 aggcactatc tggtgtgctg aagctaaaat gattcggctc tttgtcacca ggctgctctt
    10021 ctgtaacaga atagtcgatc gtacttgatt ttactcgagg cggtgttcct gttttgaacc
    10081 gttcgatagc aaagccaagt tcttttagat gattcgctag tccaatagaa ggttgagagt
    10141 tattcggacc tgatgaatat ttcaattcac cgataatgat ttctccgcga agtgctgttc
    10201 cggctgtaat aacaactgct ttggcccggt agctcgctcc tgtggacgtc actactccat
    10261 gacaaacacc atcttcgacg atcagttctt cgacgattcc ttgtcttagc gtcaaatttg
    10321 gttctttttc gatcgtatgt ttcatttctg ttgaataagc atgtttgtct gcttgtgctc
    10381 gcaatgctcg gacagctggg ccttttcctg tgttcaacat acgcatttgg atataggttt
    10441 tatcgatatt cttacccatt tctccgccta acgcatcgat ttctcgaacg acaacgcctt
    10501 tagctggtcc gcctacagaa ggattacatg gcatgaatgc taccatgtcc aaatttattg
    10561 tcagtaataa tgtttttgat cccatacgtg cagcagcaag tgcagcttct gaaccggcgt
    10621 gtcctgcgcc aacaacgatc acgtcatacg catctgcttc ataatgttcc aatcgtttgt
    10681 tcctcctttt attttcctaa acaaaattga ctgaataatt gagtgattag ctcatcttgt
    10741 acactgtcgc cgacaacttc tcctagatag tcccagcaac gcgtcatatc tatttgaacg
    10801 agatccacag gcattcctga atcaatacct gcaatgactt cttcaagtga gagggaagct
    10861 ttttctaaca acgcgatatg gcgggtatta gaaacataag tagcgtctct ttctcctgtt
    10921 tctccgctga agaataaatc agaaatagct gattccaaac gatctagtcc gtcgttttta
    10981 gctacggaaa ttgaaaaaac tggttcattt tcgatcaatt tcttcaattt ttcttgttcc
    11041 agctgtgctg gcaaatctgt tttatttaga agaatgatcc gtttcaatcc acttgtcgct
    11101 tccaataatt gttcatcttc tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc
    11161 aagtctgctt ctgctaatgc ttttcggctt ctttcgaccc cgatccgttc caccacatct
    11221 tctgtttccc gaattccagc tgtatcgatt agcttgagcg gtaccccccg aacattgaca
    11281 tactcttcga ttacatctcg agtcgttcct gcaatatccg taacgattgc tttttcttct
    11341 cttaacaaat ggtttaacaa acttgatttg ccgacatttg gccggccgat gattgccgta
    11401 cttaaccctt cccgcagaac cttcccttgc ttggatgtag caagtagcgc ttggattctt
    11461 tgttggatca tctgcgcttt ttcaagcaat aacttcgtag tcaattcttc gacatcatca
    11521 tattctggat aatcaatatt tacttctact tgtgctaatg tctctaaaat ctcttgtctc
    11581 aatgagcgga tcaaagcaga cagattgccg tctaactgat taagagctag tcccattgct
    11641 ttgtctgttt ttgcccgaat caaatccatc acagcttctg cctgtgaaag gtcaacacga
    11701 ccattcaaga atgctcgttt tgtgaattcg cctggctcag ctaatcttgc accttctctt
    11761 aacaatagct gcaaaatttg attgactaca acgatcccac cgtgacaatt gatttctact
    11821 acatcttccc gcgtaaatgt ctttggtgct ctcatcacgg agaccatgac ttcatcgaca
    11881 agctgattgc tcttgggatc aacgatatgt ccataatgga tcgtatgact tggtacttct
    11941 gataatcgtt tgtttccaga ttgataaact ttatctgcta gttgcacagc ttgatcgcca
    12001 cttaaacgaa cgatactgat tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta
    12061 tcaaattcta aagtaatttc agccattttt tcacttcctt tttcattttg cgcacaaaaa
    12121 aagtgcccac tccacccttt atgcacaata aacggtgaac ttcgcacttt gactgcttgt
    12181 tcttgattaa attctggtca tagattatca cacttgctat aaggaatcaa gtgtttaaaa
    12241 caattagatg aagttgagcc tgttctgctt ccattacctt atatttaatt gtccttggat
    12301 gtctctttgc gttctttggt ttattcgaag agaagatact gtataaacaa ggctttatct
    12361 ttcttaaaaa aaacggtaca ctaaatggta gcgctatcta aactatacac ttttgaaagg
    12421 agaaaatatg tgagcccgtt cagatctgct gcattggaag aagaaagtga ccctcggatc
    12481 aacgatgtcg ttttatctac agggaatatc aaccagaaat atatcgtccg cgatatcgtt
    12541 tttgttgtgg aatgcttaga agctgatttg tttgacccat cttttcccaa agatgacctt
    12601 ttcttaacaa cgaagaggaa attaaagacg aaggtacttg aatatggtgc gaatgcagta
    12661 atcaactgtc attttgagta tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt
    12721 tttgcttatg gaacagtgat ccaatttatc caaacaacga ttggttaaat gaaacagaca
    12781 aaaaaagcgg atcaaaactg aagacagttt cgatccgctt ttttctatca aagttagtga
    12841 ctacttcatc gtatactctg ataagaatga tttctagtaa aatatacttg gtttgaaaaa
    12901 ctttgtcaac gtgatttatt caaaaagtaa tggaatcaat aatatttttt agcaggttct
    12961 actactaaat aacgataagg ttcctcacct tccgaatgtg tttttacatg ttcatctttg
    13021 cttaatgcag catggatttg tttacgttca aatgcaggca ttggttctaa gaaaacaggc
    13081 tgccctgtcc gttttacttt ttcagccgtg cgatcagcta aacgctgaat gatctcttgg
    13141 cgcttttcac ggtaattgcc tacattgact actacagaca gcttatttct tgctaaacga
    13201 tggatgaaga cttgtgccag atattgcaat gcattcagtg tttttccatg tttcccaatc
    13261 agtatgcctt gcttttgtgt ttctaaatgg aaaacaacca tattatttga ttctctttcc
    13321 attttgacaa gagctggagc atcaagctgt tttgaaattt cagtaagata aactgccaat
    13381 tccgttaaag ctgcttcatc acctagctca cttacttctt ctgttttttc ttcaagaatc
    13441 gtttcgttga ctacctcgat ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc
    13501 tgttctactg tttcagaaac tttttcgctg atacttggct ggatcgaaag ccgtgcattc
    13561 tttcgaccca ttcctaggaa accttttttt ccttcttcca gtatttcgat ttcgacttgg
    13621 tctttggcca aacccaaatc agccagacct agttggacag cttcgtctat tgttgcagct
    13681 tcataaatcg gcattagcat tacctcccct attatttccg ttttttctta ggattcattg
    13741 ctttcttaag agcacgttct ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc
    13801 gaattttgaa agggttgttg atcagcagcg tttgtacaac ttggaaggca ttggaaacta
    13861 cccagtataa tgaaagaccg ctggctaaat tgacacccat cactaaaatc attacaggca
    13921 tcacgaaatt catgatcttc attgatgcat tcgattcaat ttgactcatg ctagataaat
    13981 acgtacttgc aaaagtaaag attgctgcta aaatcggcaa aataaatgtt ggatccgcgt
    14041 tccctaagtt caaccataag aaatgccctt gtttcaactc tggtacacgt gaaatagatt
    14101 gccataacgc catcaagatt ggcatttgta ctaataaagg aagacaacca gcatatggat
    14161 tgacgccatt ttctgcatat aaacgctgct gctcttctct taacttactt tgtgtttccg
    14221 gatctcttga actatattgt tgctgcagtg cttttaattt cggttgtaat tcttgcgttt
    14281 tacgcatgct ctttgtttgg aaatgcatca acggcaatag gatcacgcgg atgatcagtg
    14341 taaataaaat gatccctatc cctgcattac caaatgacaa cgccttgatc gcctcagcaa
    14401 agtaataaac aatataacga tcccaaattc ctgtactctg tgcgctgact tccccagttc
    14461 cgcaaccgga taagacgatg accaaactaa cgataccggc catcaaaagt aaacgtttgt
    14521 atttcttcac ttactctttt cctctctgtc gattattttt gcaagtttta agacgtggac
    14581 taaattggag cgaacctcat cataagtcaa ccccttcaca cttggtctag caatcacaat
    14641 aaaatccaac tccggatcaa tttcttcttt tatagaataa actgctgctc ggatttttcg
    14701 tttgacttca ttgcggttta ccgcgttgcc gacctttttt cctacagata aaccaactcg
    14761 aaaatgaggc tgtcccggtt tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga
    14821 atctcccgta tggaacacgg cttgaaactc tcgttctttt tttactcgat acgcttttct
    14881 cattagtttt ctttcctttt ctatctctca cttacgctca taatcatacc ataattacga
    14941 ttgtcctttc atcatttccc taaaaaagag tataaaaaaa accactgaaa ccccagtggc
    15001 ctaagctgaa agaacttttc ttcctttgcg acgacggcta gctaacacac gacgtccatt
    15061 tttagtactc atacgtttac ggaatccgtg aactttttga cgtttacgtt tatttggttg
    15121 gtatgttctt ttcatatatt ccacctccaa aatgttctta tctattttat aaacacagac
    15181 atactttgat agtataacga caaagtagtg attttgtcaa tataaaatag aagatcgatt
    15241 gttaaaactt atccacaaga ttattttttt ctatgggaat cctatgtgga aaaagttatg
    15301 cacagggatt atccacagat aataaaaggc tggttttaca cagaaaacac acgtgtaaaa
    15361 aaagtaatcc acaatcaggc gatttcctgt gtattccgtc tcctaactat tggtatatct
    15421 gtatttttta tccacagacg caagaggaaa tctattggtt tttacgagtt ttccacctgt
    15481 gcattagtgg tgtggaaaac tttttttgac aactgtgttt ttctcttttt tctttttgtc
    15541 gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg
    15601 aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag gccttatatg
    15661 gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga tttatcgcct
    15721 gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca aaaccagctc
    15781 gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct ggcgacaaaa
    15841 atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt tattactggc
    15901 gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt ggcacctagt
    15961 ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt cattggtaaa
    16021 ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg ttctatttat
    16081 aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat gcatgcaatt
    16141 ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag cagtgaaact
    16201 tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa
    16261 tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga aaaagaagct
    16321 acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa acaaatcgta
    16381 ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc
    16441 tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag aacagcgatt
    16501 ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact aagctacatc
    16561 gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct
    16621 tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc actgaaaacg
    16681 ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga agaagtggca
    16741 aagtattatc acatccaatt gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt
    16801 gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc tttaccgaaa
    16861 atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca tgaaaaaatc
    16921 cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat caaaaacttt
    16981 ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa
    17041 cgttcaaaag ttatacacac tttttccaca gctgtttttt cgtttctctc ttacgttttt
    17101 tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta actaataata
    17161 aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga gctagcttta
    17221 tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc cctattttga
    17281 caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc aacgctgata
    17341 tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta
    17401 ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga cttcctgaag
    17461 aaacatttac tttagaagtt ttagaaaata aacaagtagc gatcacttct ggaaaagcga
    17521 attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt gtcgaaagcc
    17581 ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa acagtttttg
    17641 ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt ttatctgata
    17701 attctttatt agctgtagct actgattctc accgtctaag tcaacgcgtg attccagtag
    17761 aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc gaattatctc
    17821 gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt
    17881 tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat ccagatacca
    17941 atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct agctttttag
    18001 cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc gttcgtttat
    18061 ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg aaagtagaag
    18121 aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc aatccggatt
    18181 atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa tttatctctg
    18241 ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt cagctgatta
    18301 ctcctgtacg tacaaattaa caatgatttt caatgttctt gaaaatttaa ttgaaaagaa
    18361 atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc tttctgttta
    18421 tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat atatggataa
    18481 atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat gaaaatgagg
    18541 gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt
    18601 tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca
    18661 ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga
    18721 tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg
    18781 aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt
    18841 tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa
    18901 agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt
    18961 ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt
    19021 ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacag
    19081 aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt
    19141 tctttagtaa aaggcagccc gcagatcagg cggaagtttt tagatatgga attaggccag
    19201 atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac
    19261 cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg
    19321 tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag
    19381 cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg
    19441 gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa
    19501 gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg
    19561 acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact
    19621 tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat
    19681 ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt gatgagcgag
    19741 ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc
    19801 ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt
    19861 tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag
    19921 tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg
    19981 ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg
    20041 tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc
    20101 atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg
    20161 tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg
    20221 ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat
    20281 cttctgttgt aaacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg
    20341 tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa
    20401 cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa
    20461 ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg
    20521 ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt
    20581 atgacggcgg gatcaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc
    20641 cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat
    20701 atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag
    20761 gtggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc
    20821 gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag
    20881 gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa
    20941 caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca
    21001 tgaaattctt gctggaaaac ccaagtgtgg gccggcaaat tgttgaaaaa ggattactag
    21061 cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt
    21121 tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat
    21181 gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc
    21241 gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca
    21301 tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg ggtacagggt
    21361 ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc atgactgatg
    21421 ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat cgttacatgc
    21481 gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac ggggtaaaac
    21541 aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag
    21601 tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa ggacttggag
    21661 aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg atgatggccc
    21721 gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg atgggggatc
    21781 gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag aacttagata
    21841 tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat ccatgatgtc
    21901 aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt tattgtcgca
    21961 cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat tttatacggc
    22021 atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag aatcgtcgga
    22081 gatgtcatgg gtaaatacca tcctcatgga gaccgtgcga tctatgagtc aatggtacgg
    22141 atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa ctttggttct
    22201 gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag caaaatcgct
    22261 acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa ctatgatgat
    22321 acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt gaacggaaca
    22381 acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc tgaggtagta
    22441 gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt gatggaagta
    22501 ttacctggac cagattttcc aacaggtggt ttggttatgg ggaaatcagg aatccgaaga
    22561 gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat tactgagatg
    22621 ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa taaagcaaaa
    22681 ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat cacagatttg
    22741 cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg tgacgccagc
    22801 gcatccgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc atttggattc
    22861 aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca aatccttgaa
    22921 aactatatcg aacatcaacg cgaagtcatc acacgacgga cggtctttga aaaagaaaaa
    22981 gcagaagcaa gagcacacat cttagaaggt ttgcgtattg ctttggatca tatcgatgaa
    23041 atcattgcga ttatccgcgg gtctaaatca gatgacgagg caaaagcaac aatgatcgaa
    23101 cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt acgccgattg
    23161 accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa actgattgaa
    23221 gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa gaatgaatta
    23281 gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt cggtgaggtc
    23341 ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac attgacaaac
    23401 aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag gggtggacgt
    23461 ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt ttcttgttct
    23521 acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc aaaaggatac
    23581 gaaattccag aatacgggcg tacagccaaa ggaatcccaa tcatcaattt actaggtatc
    23641 gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga agaaggccac
    23701 tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct
    23761 aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat
    23821 gtgctactga cagatggtca atctaatatc attattggaa cacataacgg ctactctgtc
    23881 actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt cagagggata
    23941 cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaaa tcaagaagta
    24001 ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa
    24061 ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct
    24121 ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt
    24181 atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg
    24241 atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca
    24301 gaacaaacag ttgaagaaca aggatcagaa gaataaccat caagactaag tgtgtaagct
    24361 agcaagaata accgattaaa aaatctggtt attcctgctg gcttttttga ttagttgaat
    24421 ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat
    24481 aattagagct tgtgagtagt ctaattaatt agataactct ccttgctctt aggaacctaa
    24541 gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg aaatcatgta
    24601 cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc
    24661 aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa aacgccgttt
    24721 cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag tatcttctcc
    24781 atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg acgatatcat
    24841 tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac aatagaaaac
    24901 gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt ctgacgaaag
    24961 atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact ttggctgtga
    25021 atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac tgtgtgattt
    25081 ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt ttaggtgtta
    25141 ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta tacgtgacag
    25201 aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa aatcgccaac
    25261 aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga aacaataact
    25321 ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat acttctgacc
    25381 cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc taaacgaatt
    25441 tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta
    25501 gactacattg ctgctaacca tattgaatat atcgattata aagatatcga attactaaaa
    25561 agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac aggcgctaaa
    25621 aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc
    25681 gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat cagctattca
    25741 attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg acgaaagtta
    25801 tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa acaatcattt
    25861 atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg tagtcttaat
    25921 tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat ggggtgttcg
    25981 ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc
    26041 ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg taggagtgat
    26101 tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat tgaaaatcaa
    26161 agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg caaaagaaaa
    26221 aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg atacagtaaa
    26281 gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc atcagacgaa
    26341 cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca ttgataaatt
    26401 agatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt ctgactggat
    26461 gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct tcgtagcaca
    26521 gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc gctttcgagc
    26581 agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt atggctcaag
    26641 aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt tagtccgtgg
    26701 aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt attatggggg
    26761 gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt
    26821 gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt ttccggatat
    26881 ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc
    26941 ttacatcgtt atcaatgaga acgagatcat tccagatgta gaacggtgtc ttgaagaaat
    27001 ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca tagagctgaa
    27061 aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat cgatctctca
    27121 gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc gtggggaaga
    27181 gtttccaagt aaacctctgc tgacttatat tgaatcttca gcttcttcag cttcagagct
    27241 agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc gttttgaggc
    27301 cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc
    27361 acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat ccttagtaca
    27421 atatttattg agctcggatt tagcgacata cttggagatc agcaatttga ttgctggaag
    27481 cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag agtataatag
    27541 tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca atgcagcttt
    27601 cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta ccggcacgat
    27661 caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa acgcagctac
    27721 tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg caatcaaaga
    27781 aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt tttacaagat
    27841 gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta tgcacaaaat
    27901 tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca
    27961 ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc taaaaagtta
    28021 aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc aggggaagac
    28081 agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa caaacaatac
    28141 aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc actaggctac
    28201 acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta
    28261 caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga aggaaaaaag
    28321 acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga
    28381 tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag aaaacgagta
    28441 taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact tttctctcat
    28501 tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga agtgtggcag
    28561 gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc tgtttttctt
    28621 gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt ttatcgtcgt
    28681 ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga agcgattgac
    28741 gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc cggtggttta
    28801 agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt ctactatgcg
    28861 aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac aggaatcgtt
    28921 acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc cgaacgcagt
    28981 atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc tgaagtactg
    29041 aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat cacaggtcta
    29101 ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga agaactaatc
    29161 attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat
    29221 gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg ggcagaatct
    29281 cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt acgtacaggt
    29341 cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct ttctcgagca
    29401 aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc gaaatgtcgg
    29461 aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt gcagttgatt
    29521 gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa
    29581 aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc tcgtggagta
    29641 gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg atcaattgaa
    29701 caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag agaaggcgga
    29761 gatgacgatg atggaaacga gccaaaaaat gacaacatca tcgaagtcat catcgagaag
    29821 aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata caataagttt
    29881 tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg agcaatcccg
    29941 agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta cttaatttaa
    30001 acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc cgaataaacg
    30061 gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg aggaactatt
    30121 ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct cttttcctta
    30181 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa catgagaagc
    30241 agttttttga aattgctcct tgtgagtcag agttaaacgg caggctcaca acctctttct
    30301 taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga a
//
LOCUS       DACEMK010000026        29110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEMK010000026 DACEMK010000000
VERSION     DACEMK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.026"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1030)
                     /locus_tag="IW715_000981"
     CDS             complement(<1..1030)
                     /locus_tag="IW715_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1021599.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IW715_000982"
     CDS             complement(1112..1801)
                     /locus_tag="IW715_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1021600.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IW715_000983"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IW715_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1021601.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IW715_000984"
     CDS             complement(2328..2831)
                     /locus_tag="IW715_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1021602.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IW715_000985"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IW715_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1021603.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IW715_000986"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IW715_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1021604.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IW715_000987"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IW715_000987"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1021605.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IW715_000988"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IW715_000988"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1021606.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IW715_000989"
     CDS             complement(6443..7462)
                     /locus_tag="IW715_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1021607.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IW715_000990"
     CDS             complement(7635..8522)
                     /locus_tag="IW715_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1021608.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IW715_000991"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IW715_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1021609.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IW715_000992"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IW715_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1021610.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IW715_000993"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IW715_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1021611.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IW715_000994"
     CDS             9753..10397
                     /locus_tag="IW715_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1021612.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IW715_000995"
     CDS             complement(10453..10956)
                     /locus_tag="IW715_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1021613.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLVLTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IW715_000996"
     CDS             11553..12518
                     /locus_tag="IW715_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1021614.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IW715_000997"
     CDS             complement(12617..13789)
                     /locus_tag="IW715_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021615.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IW715_000998"
     CDS             complement(13776..14921)
                     /locus_tag="IW715_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021616.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IW715_000999"
     CDS             complement(14935..15867)
                     /locus_tag="IW715_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021617.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IW715_001000"
     CDS             15984..17063
                     /locus_tag="IW715_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1021618.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IW715_001001"
     CDS             17060..17689
                     /locus_tag="IW715_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1021619.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IW715_001002"
     CDS             complement(17799..18026)
                     /locus_tag="IW715_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1021620.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IW715_001003"
     CDS             complement(18129..20717)
                     /locus_tag="IW715_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1021621.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IW715_001004"
     CDS             complement(20737..21396)
                     /locus_tag="IW715_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1021622.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IW715_001005"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IW715_001005"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1021623.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IW715_001006"
     CDS             complement(22169..23035)
                     /locus_tag="IW715_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1021624.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IW715_001007"
     CDS             23144..23785
                     /locus_tag="IW715_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1021625.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IW715_001008"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IW715_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1021626.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IW715_001009"
     CDS             complement(25226..26125)
                     /locus_tag="IW715_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1021627.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IW715_001010"
     CDS             complement(26127..26924)
                     /locus_tag="IW715_001010"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1021628.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IW715_001011"
     CDS             complement(26902..27606)
                     /locus_tag="IW715_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1021629.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IW715_001012"
     CDS             27737..28312
                     /locus_tag="IW715_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1021630.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="IW715_001013"
     CDS             28430..29107
                     /locus_tag="IW715_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1021631.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8567 a         6169 c         5231 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acactagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa
//
LOCUS       DACEMK010000027        29050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEMK010000027 DACEMK010000000
VERSION     DACEMK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.027"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..1112)
                     /locus_tag="IW715_001014"
     CDS             complement(48..1112)
                     /locus_tag="IW715_001014"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1021632.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1109..2194)
                     /locus_tag="IW715_001015"
     CDS             complement(1109..2194)
                     /locus_tag="IW715_001015"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1021633.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IW715_001016"
     CDS             complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IW715_001016"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1021634.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IW715_001017"
     CDS             complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IW715_001017"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1021635.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4443..5096
                     /locus_tag="IW715_001018"
     CDS             4443..5096
                     /locus_tag="IW715_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1021636.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            5181..6086
                     /locus_tag="IW715_001019"
     CDS             5181..6086
                     /locus_tag="IW715_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1021637.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(6088..6720)
                     /locus_tag="IW715_001020"
     CDS             complement(6088..6720)
                     /locus_tag="IW715_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1021638.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7040..7564
                     /gene="infC"
                     /locus_tag="IW715_001021"
     CDS             7040..7564
                     /gene="infC"
                     /locus_tag="IW715_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1021639.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7636..7836
                     /gene="rpmI"
                     /locus_tag="IW715_001022"
     CDS             7636..7836
                     /gene="rpmI"
                     /locus_tag="IW715_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1021640.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7889..8248
                     /gene="rplT"
                     /locus_tag="IW715_001023"
     CDS             7889..8248
                     /gene="rplT"
                     /locus_tag="IW715_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1021641.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8600..9838
                     /locus_tag="IW715_001024"
     CDS             8600..9838
                     /locus_tag="IW715_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1021642.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            9886..12015
                     /locus_tag="IW715_001025"
     CDS             9886..12015
                     /locus_tag="IW715_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1021643.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11990..12679
                     /locus_tag="IW715_001026"
     CDS             11990..12679
                     /locus_tag="IW715_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021644.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(13087..13773)
                     /locus_tag="IW715_001027"
     CDS             complement(13087..13773)
                     /locus_tag="IW715_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1021645.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13909..14103)
                     /locus_tag="IW715_001028"
     CDS             complement(13909..14103)
                     /locus_tag="IW715_001028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021646.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14153..14593)
                     /locus_tag="IW715_001029"
     CDS             complement(14153..14593)
                     /locus_tag="IW715_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1021647.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(14597..15442)
                     /locus_tag="IW715_001030"
     CDS             complement(14597..15442)
                     /locus_tag="IW715_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1021648.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15591..17009
                     /gene="pepV"
                     /locus_tag="IW715_001031"
     CDS             15591..17009
                     /gene="pepV"
                     /locus_tag="IW715_001031"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1021649.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17085..18287)
                     /locus_tag="IW715_001032"
     CDS             complement(17085..18287)
                     /locus_tag="IW715_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021650.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(18274..20061)
                     /locus_tag="IW715_001033"
     CDS             complement(18274..20061)
                     /locus_tag="IW715_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021651.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(20074..21027)
                     /locus_tag="IW715_001034"
     CDS             complement(20074..21027)
                     /locus_tag="IW715_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1021652.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(21212..21562)
                     /locus_tag="IW715_001035"
     CDS             complement(21212..21562)
                     /locus_tag="IW715_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1021653.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            21762..22841
                     /gene="pepA"
                     /locus_tag="IW715_001036"
     CDS             21762..22841
                     /gene="pepA"
                     /locus_tag="IW715_001036"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1021654.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            22984..23304
                     /locus_tag="IW715_001037"
     CDS             22984..23304
                     /locus_tag="IW715_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1021655.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            23326..23790
                     /locus_tag="IW715_001038"
     CDS             23326..23790
                     /locus_tag="IW715_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1021656.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            23986..24591
                     /locus_tag="IW715_001039"
     CDS             23986..24591
                     /locus_tag="IW715_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1021657.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(24630..25919)
                     /locus_tag="IW715_001040"
     CDS             complement(24630..25919)
                     /locus_tag="IW715_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1021658.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            26347..26826
                     /gene="rlmH"
                     /locus_tag="IW715_001041"
     CDS             26347..26826
                     /gene="rlmH"
                     /locus_tag="IW715_001041"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1021659.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            27179..27493
                     /locus_tag="IW715_001042"
     CDS             27179..27493
                     /locus_tag="IW715_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1021660.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            27509..27895
                     /locus_tag="IW715_001043"
     CDS             27509..27895
                     /locus_tag="IW715_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1021661.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            27924..>29050
                     /locus_tag="IW715_001044"
     CDS             27924..>29050
                     /locus_tag="IW715_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1021662.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9195 a         5449 c         5878 g         8528 t
ORIGIN      
        1 tgcttccgcc gtttatacgt gtttagagcg tgggataaaa gtgttcttta cttttgtccc
       61 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
      121 tccttcactg gtccggaaaa gatcagtgat tgttggtgta gggccgcggt attcgttgcg
      181 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
      241 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cactgcattt
      301 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
      361 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
      421 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
      481 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
      541 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
      601 gtttgagtct cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
      661 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
      721 gcttcttggg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
      781 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggtcttt caaggcagcg
      841 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
      901 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
      961 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
     1021 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
     1081 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
     1141 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaaggatgc
     1201 cagatttctg gtcaacaatt acgattccgc aatcgccacc acctgcacca gaagattttg
     1261 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
     1321 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
     1381 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
     1441 tgttttttaa gaaaagctga taagccacca ttttgtcttc tctcgaacgg tgaacttgat
     1501 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
     1561 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
     1621 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
     1681 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
     1741 tggctagtgc tgcaatcttg aaaatctcca attgagataa attcaacgcg taaaaaacat
     1801 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
     1861 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
     1921 tttcttgtgc atacttttca gtcaaacgaa tagcagcaag gatataatga aaaggatttt
     1981 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
     2041 attgtgcaga ttggatactt ccgacttttc gtgcggattc tacagtgact gttacgaatt
     2101 gatcgactgc agcgataact gctggatgtc ctgtttcaac aactgcatat tctccggcaa
     2161 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
     2221 caattcctgg accagcatgg gcagtaatta cttgttcttt gctaaataat ttggtaaatg
     2281 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
     2341 ccatcgtaaa gtagcatgga atgccttttt ttctcaattg ccgcaccgca tccatcgctt
     2401 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
     2461 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
     2521 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
     2581 atgtttcgac tgttcgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
     2641 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
     2701 aagaacttaa gtcgtcatgg cctttttccc attcaacgaa accaccgaaa atacttcggc
     2761 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcgtct aatccaagct
     2821 taagagcagt attgcaagct cctgctaaag cagctagccc gctggcagag gaggctaatc
     2881 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
     2941 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgtaagg
     3001 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
     3061 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
     3121 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
     3181 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
     3241 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
     3301 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
     3361 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgactgtga ttcatccatt
     3421 gtctgtgcca acgtttctgg tgaattctca atgattgctt gttttgcttg cttcgtcaag
     3481 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
     3541 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
     3601 gcatcgatgt tcaaagaaaa gtagtcaaaa ggatgccctt ttgtaaaata aatcggctgc
     3661 aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa
     3721 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
     3781 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
     3841 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
     3901 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
     3961 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
     4021 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
     4081 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
     4141 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
     4201 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
     4261 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
     4321 actttttttc ttctttgact agaaaaaata caattgacac tactagatat tgggggtata
     4381 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
     4441 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
     4501 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
     4561 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
     4621 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
     4681 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
     4741 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
     4801 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
     4861 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
     4921 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
     4981 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
     5041 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
     5101 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
     5161 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
     5221 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
     5281 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
     5341 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     5401 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     5461 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     5521 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     5581 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     5641 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     5701 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     5761 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     5821 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     5881 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     5941 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     6001 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     6061 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     6121 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     6181 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     6241 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     6301 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     6361 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     6421 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     6481 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     6541 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     6601 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     6661 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     6721 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg caagaaagcc
     6781 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     6841 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     6901 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     6961 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     7021 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     7081 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     7141 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     7201 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     7261 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     7321 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     7381 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     7441 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     7501 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     7561 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     7621 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     7681 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     7741 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     7801 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     7861 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     7921 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     7981 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     8041 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     8101 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     8161 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     8221 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     8281 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     8341 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     8401 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     8461 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     8521 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     8581 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     8641 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     8701 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     8761 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     8821 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     8881 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     8941 cttgatgtca ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat
     9001 ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt
     9061 caatccagcg gctgaaagct atacttataa tgactatttc caaattggta tgattgggat
     9121 gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg
     9181 ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt
     9241 agccgtatta ttaccagttt taggggtcgt tcttttagat cttcaaatca tcttaggttt
     9301 tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga
     9361 aatctgtcgt cagtttgcga aattatttac agatggcgca gtcgatgttg caccaatggt
     9421 tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt
     9481 ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc
     9541 tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc
     9601 tattttagct gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt
     9661 atttgcaatt acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg
     9721 gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg
     9781 ttggatcatc ggtgcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta
     9841 aacaaaacgt cgataaaaac gattagataa atggaggaaa agaaaatgaa acgttttgtg
     9901 cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg
     9961 cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc
    10021 gcaggtgtcg ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat
    10081 gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga aggagatgta
    10141 gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga
    10201 caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca
    10261 ttcttacctg tcaaaaactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa
    10321 agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca
    10381 gaacgagttg tttatgaagc agcatctgtg aaagcgatcc ctacaacgat ggcttctgac
    10441 atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa cgcatctatt
    10501 ttaccaaaaa tgctagatac ggcaacctcg acagaagact ccgtacgaga agcaggggtc
    10561 aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa
    10621 aaacgacctg ttttaaccat gctttccggt cctgctgcat cagtcatggg ctctttgatg
    10681 tacttgagag cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg
    10741 gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat
    10801 atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat
    10861 cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca
    10921 gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa
    10981 ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca
    11041 aatacatgtg cagccaatgt tcttggatta gtgcaagaag aacatttttc ttatggaaat
    11101 gtcccatctg cacgaaaagc catacaggca ttagcagact attgtcatac gacggttgaa
    11161 gacatcgcag agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa
    11221 ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc
    11281 gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa
    11341 aacgccgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa
    11401 cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat
    11461 aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca
    11521 catacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta
    11581 accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat
    11641 aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat
    11701 cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa
    11761 cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa
    11821 ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc
    11881 tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg
    11941 atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat
    12001 atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat
    12061 ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca
    12121 gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa
    12181 aaagtaccct tttgccgatg taggagaaat ctatgaacaa atggctgttc caatcgtttc
    12241 ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg
    12301 gatccgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc
    12361 taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc
    12421 tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac
    12481 acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat
    12541 gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga
    12601 aattcgtagc caagggatct tagaagaaat aaaagaaata gaaaaaagat gtacggagta
    12661 tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg
    12721 aataaacggt gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga
    12781 gaagctgttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
    12841 ttggaaaaag gtgttcaaaa gcttgcatct gcggtaataa gaacagccaa gttaaaaaca
    12901 taagaagcta tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac
    12961 ctctcaataa acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa
    13021 atatgaggaa ctattttgcc gtttatttct ctaatactca ggtcaaaacg ctgattccaa
    13081 aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat
    13141 cacgtattct tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc
    13201 atcaaagtta tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc
    13261 ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata
    13321 actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa
    13381 atcagtatcc aaatcaaaaa aagctttttt attttgagcg atcactgcct gatcggtttg
    13441 ttctagtatt ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg
    13501 caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc
    13561 gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag
    13621 ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt
    13681 atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc
    13741 ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa
    13801 tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa
    13861 agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg
    13921 tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt
    13981 tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt
    14041 atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt
    14101 cattatacga actactcgac acaatacaag aaaaatgctt agacggtatt tattaagaat
    14161 ttacatcatc caaagaagcg tacgcttgga ataactgttc taactccgga ttgactttgc
    14221 tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag
    14281 tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgttcccg
    14341 tatacttttg gatagttggg tggatgctca ctttttctcc gtaatggatc atttctttat
    14401 agctgcaatt tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg
    14461 gaaaccctag atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc
    14521 tgtgatgaat aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg
    14581 taaatttctc catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc
    14641 acttcggaag tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct
    14701 ggtctccaat gaaactatga agataaacag ctgctgcaat cgtttggtca ttttgaggaa
    14761 actgggctaa aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cctgttgcca
    14821 tagcagcaga acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat
    14881 gacttttcaa aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc
    14941 gttgttcttg tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta
    15001 cagtttgttc gggatagggt agcaaaagat gattagcaac aagtaaactt aaagcagagc
    15061 catcaatcac cagccattgt tcttttgatt gtttttctag tacttgtttg aaacgttcca
    15121 aactcttaga agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta
    15181 cggaaagaaa ctggtcagta tccgtccaat ctacaatcat tgcttctggc agatgactgt
    15241 gaagagcact ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccactgtgta
    15301 cggcagctaa agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa
    15361 cgatacgtcc aaatgttcct ttatgtgaaa tctttggacg aggtgtgatg actcttttca
    15421 ctagttgttc gtttatcagc atcgaaaaac ctccttttac tcattatagc gtatattttt
    15481 tttagagaaa aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta
    15541 atttgtggta tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg
    15601 attggcaaaa agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt
    15661 tacgtgtcaa cagcgagcgc gatgattcaa aagtaacacc agatgcacca tttggacctg
    15721 gacctcgcga cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa
    15781 aaaatgtcga taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga
    15841 tatttggaca catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac
    15901 cagtaatcaa ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta
    15961 tggcagctta ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag
    16021 ttcgcttcgt agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg
    16081 aacatgaaga agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg
    16141 gtgaaaaagg aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg
    16201 tcttgaaatc ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag
    16261 cacttcaagt accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca
    16321 tcgaagcaaa tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat
    16381 tagttggtaa aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct
    16441 tggcattgtt cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag
    16501 cagctgctta tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg
    16561 aaaaaatggg caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag
    16621 aggaagcgaa cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat
    16681 tgcaaagtga catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc
    16741 gcgtaatgga accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag
    16801 tctatgaaga ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg
    16861 gacgtttgtt gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa
    16921 tgcatcaagc aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg
    16981 cagatgccat ctatcgtttg gcgaaataaa aatactaaaa aactccggct atctctgaca
    17041 gctggagttt ttatgtttaa aaatgaatta gtacatcggt attcttaaaa gtaatttagt
    17101 tcgtcttttg atttctcagg agggatcaaa aaggcgaaag cactaagtat agcaaaactt
    17161 gtgatgattt gcgtacgata cgtaaaccaa taatgaatct gtgagtgatt agctaaaata
    17221 ttgtaccaaa tatacggcgt tacagcagct aaaacaaaca aaactagaag ttggaacgga
    17281 cgccagctat ttcgtttacg ataagcaaaa tagagaaaaa caagacaagt aatcaatagc
    17341 aataatattg ctacttttgg atacatcaat ctaatattgc ttttcagcat ctctaatcga
    17401 tgcaacgggt atttgtcgtt tccttccgtt cgaaagagga tttggttgat tgcatctttt
    17461 acaatgtttt tgttcaatat gatcgatgat aggatccatt tagccaccca tgtcaaacca
    17521 tagcctaatc cccaaaagat accggtaaaa acaaaactgc ctacttgttt gctcaatgaa
    17581 ctgcgtttat ccattaaata cttatttgtt acataataaa taatgatcac aggtatcccc
    17641 caagagatga ccggtgttgt caacaagtca aaaaagttag ctaaagaacc aatgacaaag
    17701 aaatacaaga ataattgttg gttggtgttg gagagttgtg gttttgttaa aaatagtaat
    17761 acagacaata aagtcaaaat ccaaatattt gaaaattgca tactgaagaa aaaggttgca
    17821 gcatttccta tagcaagcga aatcaacaag gctgtcgtta aatagatgtc gctacacttg
    17881 gttaaaaagt agctcgtcaa tccaatcagc agaaaaagaa tggctgcgta aatcattcga
    17941 atactcccca aactcatccc tagcagtaaa ggtcgtaaaa aaagctgata accatgccaa
    18001 taacggggat aatccgccat agacatcgcg ttctccaagg agttcaattc aggatctgct
    18061 tttgtttttc gtatcatcaa atgatcagta aaattatcta accgagtgcc tagcatattt
    18121 ccttgattga tagaaggata cattccttct ttttcaacca tctgtagaga tttttctgca
    18181 ttcgattcaa tagagtgctg cggaatgaaa aaagtaattg tcagtagtac gcaatagcta
    18241 gcgatcaaga gaacaaaagc aaaaaatatc tttttaaata gtttcatgag tatctcctat
    18301 atctgttttc attttttctc ttttatctag taaaaaagga gagaggatat aaacaggtag
    18361 acctaatgct aaaacaaaca cataccgtaa actaaaagcc acaggagtag caatcataat
    18421 agtagcaaaa acaagcatac cgggaagaaa aaggagtaaa taccgatatt ttttcttaaa
    18481 gattgctaaa agtgcactgc cgatagctaa ccacagtaat gtgccggaac caaagaaatc
    18541 acgagcatcg atgatttttt tcattcctga caatccagta agatgttcaa tcaaatctac
    18601 ttgcttcata ccataattat tcgtgtttac acgagtatct aaaaagccgt aactattttt
    18661 tgtaccaggt acccagaacc cgaacgtttc taaaatataa gcatctgtat agatttttat
    18721 attacttggt aaatttcttg cccatagtga tagaaattct tttttatgtg tattcaaaaa
    18781 ctcattgtta aaatttttat gccacttgat tgagtctgca agactaggag aatacgcttc
    18841 ataatctttt atcgggagta gctgattcat gaatgcttta tctttcttag acaagtcacc
    18901 atccataaca atagttctag aaatttgctg caaaggaaca cctaattgtt cagcagtttc
    18961 tgtaggaata gcataacgag ccatcaacgg gtggatgagt agtccagcaa tcactgtata
    19021 acccagcacc acttgaaaga atgcttgata ccttttttgt ttcaaaataa tcaagccgca
    19081 aatcaaacct gtgccggtta ctacataaat cccattattt cttagatagg tggtcagtaa
    19141 agcaaggatg attacgtaaa tctgattgag aatcgttggc ttttctcgtt gaaaaataaa
    19201 gtcaaaaacc gctaaggaaa ataaaaagca agcgatagag aacaaagtat ctttttgcat
    19261 gttcaaggcg tagattggaa aaaccggtgc aaatgcaaaa tagcataaac taatgagtac
    19321 cggaaaaaaa tggatgtttc tttttaacag ccagcaaaga gaataactta tcgctgaagc
    19381 tgttatcaat gtttgaaaaa tcgaaaataa aaatacaccg tgattgatcg tatcagttgg
    19441 taaatcatga acaaatagct tgaccagtaa ggtgaataag actggatgat gattgcttat
    19501 aggaacatca cccatagatt gggcgaccga acttaacgaa tcaggcaaaa ccactccagg
    19561 atacaatgcc aacagataag gaagccaaca cagaactaaa aagacagtaa agaataaaaa
    19621 atattttctt gaagagaaag cctgtgtgtt ttgagcagta aaaaatgttt ccagttttgg
    19681 cattgataaa taaatcgatt gtaaaaataa aaaagaatac acaaaaatgg agaaataaac
    19741 aagaaagtaa aaaatacttc catcagcaat cgttgtcttt gtatagtttg ctgagacata
    19801 ctgaaattgt atctgtctgc caatagaaag tgtaagagca aaagctgcag aaaaaaacaa
    19861 tcgggcaata gctgcaactt tgttttttat tgaaaacaac tctattccat attgcttaaa
    19921 agcaagtagc aaaaccatgg ctaaaccaat gattactgtt ggattcaaag ttcctagacc
    19981 ataagaagta aaaaaagcaa aacctgacgc aaataaagtt gcaaaaaaag taggaaatag
    20041 attctccttt ttcttatgca taaaaaaggc ctcctacttc atttctttat tttgatattc
    20101 gtggtagaca tggtataaat tcaattcata ttctttcttg gcatttgtga cgaccgtatc
    20161 caaaatcaac cctgttacta atgaaagaag ggctaaagtc atcaatccag tacttaaaat
    20221 cgcagaaggg atgcgtgtaa taaagcttgt cagcatatat tcacgaatca caggaatccc
    20281 catgaccaat ccaaataaaa agaagaagat ggtccagatc ccaaagaaaa gcaaaggttt
    20341 ataatctttg cacattttga tgatcatcat gatcactttg aatccgtcag agaatgtatt
    20401 caattttgat tcgctacctt caggacgatc gcgataatcg attggaatct caacgaactt
    20461 gaatttttta tctaaagcat gaatcgtcaa ttctgtttcg atttgaaaac cagaactcat
    20521 aatagggaag cttttgacaa aaattcgatt gaatcctcga tagccggtca tgacatcgcg
    20581 aatttttgtt ttatataagt aagtaattgt atttttaacc agattattcc caaaatcatg
    20641 gaaaggacgc ttattttcat caaaataggt tccatttgat agacgatcgc caatgaccat
    20701 atctgcttcg ccagaacgta atttctcaag caaaccatgt gcagcttctg caggataagt
    20761 atcatcacca tctaccatca aataataatc ggcatcaata tcaaaaaaca tttgacggat
    20821 tacattccct tttccttgac gtggttcttt ctttacgatt gctccgcctt gaacggccaa
    20881 ttcgtatgtt cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc
    20941 ccttttaaaa tcttcaataa cagttgaaat tgtcgcctct tcgttataac aaggaatgag
    21001 cacggcaatt ttttcacgtg attccatttg tcactctcct tatataataa aactaatctt
    21061 atttcaataa taccgtataa taactacaaa tactagctat tccaagagga aatagcccta
    21121 tttttataaa aatcacccac tataaaagaa ggataggata cctctctttt agatagtggg
    21181 tgcgtattcg ttatttcatt tttttgacgt attaatctat tgcgcccttt tttcttgaaa
    21241 tgtctaatac ggttcctgta tgagcatctg ctaaaaattc ataataaaca gtttcgccat
    21301 cttctatccg ggaaagccca ccccgataag cactcgaccg aacggcgaat ttccgaatag
    21361 gttctttttc aaaagagatc caagcacctt cgattgcgcc ttcttttaaa aaagatgttt
    21421 tgatattttc aagcacttgg tctgctgata acactttttt gcgatgatac caaatcgttg
    21481 aagctacccc actcaataat cctagaccga tcccaacggc taacccgcct ttaaaatagc
    21541 ttaattcgct ttcttcatac atgtggatat acctccttct tctcacttgc tactctttgt
    21601 gtttatttta ccacactctc atattttctt tgaaataaat aaccgctacc agaaaaaata
    21661 agctataata aatacgatcg gtctgcagaa aaaatcatat tttatccgca gatcaatata
    21721 agtctgagag gagtttagtc tgaaatttag acgaatagca tatggaagaa aaaacatttc
    21781 aacgaatcaa agagttaaca gaattacaag gaacaagcgg ttttgaacaa gacgttcgcg
    21841 cctatatgga aagtcatatg gaaccgctgg tagatgagtt acagttagat ggtttgggcg
    21901 gcatttttgg gctgcgccac catgaagaag cggatgcacc tcgtgtaatg gtcgcagcac
    21961 atatggatga agtcggattc atgctgacac aaatccaaga aaacgggctt tttaaagtcg
    22021 tacctctagg cggatggaac ccttacgttg tttctgctca acgttttaca cttaaaacct
    22081 ctactggtaa gaattatcca tgtatttctt cttccgttcc cccacattta ttacgaggaa
    22141 caagcggaca aaaatcagta gaagtaacag atattttgtt cgatgctggg tttgaatcaa
    22201 gagaagaggc aatgagttac ggtgttcttc caggagacac aatcgttcct tacgctgaaa
    22261 cgatcaaaac agccaacggg aagaatatca tcagcaaatc gtgggacaac cgctatggct
    22321 gtacaatggt attagaagct ttagaagcgt tacaaaatga aacattaggt catacattga
    22381 ttgcaggcgc gaatgtccaa gaagaagtag gcttgcgtgg ttctaaagct tctgtcaata
    22441 aattcaagcc agatctgttc tttgcagtag actgctctgc agcagatgac acagtgacga
    22501 aaaatggcac attcggacat ttaggagaag gtacattgat gcgtatccaa gatccaggtt
    22561 tgatcatgct tcctcgtttg cgtgagtact tgctagatat cgctgaaaca aacaacatcc
    22621 cttatcaata ctttgtttca aaaggcggta cagatgcagg tgctgctcat acacaaaatg
    22681 aaggaatccc aagtacagtg attggcgtag tgggacgtta tatccatacg catcaaacga
    22741 tgttcagtat tcgcgatttt gaagctgcac gagaaatgtt gattcaaaca ttgaaaggtt
    22801 tagataaatc aacagtgaac acgatcgtat acgggaaata acgcgatagt gaaactataa
    22861 aatctggcaa aagcaacctt ttgtcagatt ttttttataa aaagaggatc aatgtttgtt
    22921 agttcgaaag aaaggtgtat aatttaattg aaaaatacat aaacagaaag tagaggaaaa
    22981 agaatgatta tcccaaatag tttagaagaa ttagcaggct acgtagaaac tggaaaaagt
    23041 gtatttttct ttactgccgg ctggtgcgga gactgccgtt ttatcaaacc gcaaatgcct
    23101 gaaatcgaaa atgacttttc tgattggcaa tttattgaag tagaccgtga caagtacata
    23161 gatgtagccg ctgaatggaa catttttggt atcccaagtt ttgtcgtgat ccaagacgga
    23221 aaagaattag gtcgtctagt gaataaagat cgtaaaacaa aacaggaaat cgaatcgttc
    23281 ctagaaagtg taataaaagg ctgacatctg ttaggagggg tcaagatgaa tcaagaatat
    23341 aagaacattt tagtaggtat tgacggtagt gaacaagcac ttgctgcttt caaaaaagcg
    23401 ttagaagtgg ctcgccgtaa taatgggaaa gtatatgtag caaacgtaat cgatcagcaa
    23461 ttttataatt ttatgggcta tgctcctatt gatcaaaatc tagtcgatca gcaaactgaa
    23521 acagccaagc aaatgatcga agaatgtaaa gaatatggaa aatctgttga ttacacaaat
    23581 atagaaggaa ttattgcata cggctctgtt cgtgaatcca tggcagtcaa attgccaaaa
    23641 aaatatgcaa tcgatctaat catggttggg cagtctggac taaatgcagt cgaacgcttt
    23701 atgacaggaa gtgtagccag ctacgtcata cgtcattcac cttgtgatgt cttgatcgtt
    23761 tctgacgaag aagaacagcc aagtaattaa gcaagtggaa cttgtataac ctttttaatc
    23821 aaacagtaaa gggactgtga caaaagtctt gtcacagtcc cttgttacga ataaatggtg
    23881 ttccagaaga agcttcttcg gaaataaagc atgaaaaaaa tctcatgcag tgaatgcaat
    23941 tttttgttaa gataaatctc agcaaaggat aatggaggaa agaacatgat ttttgcttat
    24001 aacaaagcac acgtcggaga cactttgatg gttatcatag ccgacgataa aggaacagaa
    24061 aacactgtcg aacgtaaatg gaatgttgct cggatcaaag atgaacaagg tcaagttgtt
    24121 gcttggaact ttttccatat ctccgatcat ctaacaattg aagggaacgg ccaagtaagc
    24181 ttaacagatg aagaattagc gaaattgaat cgattgatta aagaagcagg attcgaagaa
    24241 cagcttgaag ctgatcatga accgaagatc gttgtcgggt atgtaaaaac atgcgtcccg
    24301 catcctgact ctgaccattt atcgattact gagacagaag tggacaatgg gacagtattg
    24361 caaattgttt gcggtgcacc aaatatcgaa gctggacaaa aagttgtcgt tgcaaaacca
    24421 ggagccatga tgccagatgg aatgatgatc tggccaggaa ctttgcgagg ggtagccagc
    24481 tatggaatga tttgttctgc tcgtgaattg catttgccaa atgctccaga aaaaaaagga
    24541 atcttagtat tgcctgagga tgccgtagtc ggagaagtat ttccaaaata aagaggttgt
    24601 gacaagtctt tgtcacaacc tctttattat taattttcac tattttgatt ttgttttaat
    24661 gccgattgat cgatcgtcaa ttcaacagta gttgtttttt ctttcgagcc ttcatagtaa
    24721 gtgattttga cagaatctcc cacttttttc ttatacaaag cagattgaag ttctacacca
    24781 gaagaaactt ctttgtcatc gatcttagta atgacgtcgt attgtttcaa cccagctttt
    24841 tctgccggtg tagctgtttg gacatttgtc acgatgacac cgttagttac agatgaagga
    24901 atcttcaaga tttgttcttg ttgctgagta gaaacggcag ataagtcgac cattgtgata
    24961 cctagagctg gacgtgtcac ttttccgtct ttttccagtt ggttgatgat attcactaca
    25021 tcgttacttg ggatggcaaa tcccattcct tctacgctta cattagaagt cgattcagaa
    25081 gtacttgcga tcttacttga gttgataccg ataacttgtc cttcgatatt cactaaagga
    25141 ccaccagaat ttcccgggtt gatcgctgca tctgtttgga tcgcattgat attgactgtt
    25201 tcgttcgatt cattcgtact tgttacttgt cggttcaagg aagaaatgat tcctgaagtg
    25261 acagaattcg catattcaga tcctaatggg gaaccaatcg caatcgcagg ttcaccaacc
    25321 ttcaaagcac ttgagtcccc aaaagaggca accgtttcga ctttgtctga attgatcttc
    25381 aacaccgcta aatctgaata ggcatctgtt ccaactaatt cagcttttac cttcgtaccg
    25441 tctttcatca tgacttccaa accttgctgt ccatctacta cgtgattgtt tgtgactaca
    25501 taagcagtat tcccgtcttt tttgtagatt acgccgcttc cttcactata agcttccaaa
    25561 ttactatcat ctgagctgct actttcttct tgctgcccga ataatccgcc aaaaccgctt
    25621 gattggtttt gactttgtaa gttgataaca gaaacgacag cgtcttgaac tttatctacc
    25681 gctttggtga tatcagaatc tacattgact ttgacatttt ccacaactgt ttcaccggca
    25741 gaattttgat tgccgcttgt agcagtagaa ctattgttgc cagtgcccat cgctgcataa
    25801 aaaatgccag ctgtcagtag tcctccaacg atccctccaa caagccccag acccaactta
    25861 cgccagaggc tattattttt attttttttc atctttggtg tcacattttt tctatccatg
    25921 tgttcctcct ccaattacat tcatttatct actgttagta tagtcttatt tgttgacttt
    25981 agtctacttt gagactttga actttttatg aaaaacttta ggaaaaatca ttgttttcag
    26041 atgaaaatat ttgattttct ttttccacag atattgtgta aaactacgcg gataggattg
    26101 gaaaaataag ggataaaaca tatccacagc ctgtgtataa agtggataaa ttcttataaa
    26161 acgtatgttt gcctaatgaa tgtgatgaaa taaggtaaaa caacgctgtt ttctgtggat
    26221 aaacctgtgg atgttgtgca taactgttgg ggaaatcttt ttaacaaaaa tagtcaaaat
    26281 aaacatatag acgaaaaaat tgattataat aaacaaagtg agcgtgaaga tcaaaggaga
    26341 gaaaacatga atatcaaaat tatttcagta ggaaaattaa aagaaaaata cttgatccaa
    26401 ggtatcaatg agtatgtcaa acggctgaat gcctatgcga aaatcgaatt gatcgaagtt
    26461 cctgacgaaa aagccccgga gaatttaagc gaagcgcaaa tgagacaagt aaaagaaaaa
    26521 gaaggagaac ggatcttagc gaaaatcaaa gaacaagaat acgtctatgg cttagcaatt
    26581 gaagggaaaa atcccacaag cgaatctttt gctaaacaaa tcgaccaatt aggtatccaa
    26641 ggaaaaagcc accttgtttt tgttatcggc ggttctttag gactcagcga agccgtcatg
    26701 aaaagaagca atgctcagat ctcttttggc aaaatgacgt atccacatca attgatgcgc
    26761 ctgatcctcg tcgagcaaat ctaccgtgcc ttccggatca acgcgggggc tccataccac
    26821 aaataatgat attttttaag agaaaaatag cataaaaatc tagttatccg cataaaaact
    26881 ggacttatca cactttatca aggtcaaaac cactcaattt actactaatt tactacttat
    26941 gaatgagctt tgatacgacg atttatcctt gaaaagtgaa gatataaaga tacttccaat
    27001 aaaatttgaa tatttaatag gtagacactt caaaaaatga ggtgtctatt tttttacccg
    27061 attttgaaag gaagtgaact tatgaaaaca aaaaatcaag aatcaaaagg tcgttcccca
    27121 ctctttaaga ccatcaaaca ttcattcagc caataaaaaa gaaaggatag gtaaaaatat
    27181 ggaacttaaa tttgtgattc ccaacatgga aaaaacattc ggcaatttag aatttgctgg
    27241 cgaggataaa gtcgttcagc gaagaatcaa cggacggcta actgtcttat caagaagcta
    27301 taatctctat tctgatgttc aaagagcaga tgatattgtg gtggtgcttc ctgctgaagc
    27361 tggcgaaaaa catttcggct ttgaggaacg tgtgaagtta gtcaatccac gtattaccgc
    27421 agagggctac aaaatcggca ctcgtggttt tacaaattac cttttacatg ctgacgacat
    27481 gataaaagaa taaagaaaga gaggaaaaat gatgagatta gcaaatggca ttgtattaga
    27541 taaagacacg acttttggag aattgaaatt ctctgctcta cgtcgtgaag tgagaatcca
    27601 aaatgaagac gggtcggttt cagatgaaat caaggaacgt acctatgact taaaatccaa
    27661 aggacaagga cgcatgattc aagtaagtat tcctgccagc gtgcctttga aagagtttga
    27721 ttataacgca cgggtggaac ttatcaatcc cattgcggac accgttgcta ctgccaccta
    27781 tcaaggagca gatgttgact ggtatatcaa ggcagacgat attgtgctga caaaggattc
    27841 tagttcattc aaagctcaac cacaagcaaa gaaagaaccg acacaagaca aatagtcgct
    27901 aggtagaaag gagacttttt cgcatgaaac agcgtggtaa aaggattcgc ccatctggta
    27961 aagatttagt ctttcatttt acgatagcgt cactcctgcc tgttttcctg ctggttgtcg
    28021 gactgtttca tgtgaagaca atccagcaga tcaactggca ggattttaac ctatcacaag
    28081 cagataagat tgacattccc tatttaatta tcagtttcag tgtcgcaatt cttatctgct
    28141 tgctggtagc gtttgtattc aaacgggttc gctatgatac ggttaaacaa ctttaccacc
    28201 gtcaaaaact ggcaaagatg atacttgaaa acaagtggta tgaatctgaa caggtcaaaa
    28261 cagagggttt ctttaaagat agtgctggtc gtacaaagga aaagataacc tacttcccta
    28321 aaatgtatta tcgacttaaa aatggcttga tacagatacg ggtggaaatc acgctgggaa
    28381 aatatcaaga ccaactctta cacttggaaa agaaattaga gagtggcttg tactgtgagc
    28441 tgacggataa agagttaaag gattcctatg tggaatatac tttgctctat gacaccatag
    28501 ccagtcgtat ttctattgat gaagtagaag ctaaagatgg taaacttcgc ttaatgaaaa
    28561 acgtatggtg ggaatatgat aagctccctc atatgttgat tgctggtggt acaggtggcg
    28621 gtaaaactta ctttatactg acactgattg aagccttgct tcatacagat tcaaaactgt
    28681 atattcttga cccgaaaaat gctgaccttg cggacttagg ttctgtgatg gcaaatgtct
    28741 actatagaaa agaagacttg ctttcttgca ttgaaacatt ctatgaagaa atgatgaaac
    28801 gtagtgagga aatgaagcag atgaagaact ataagactgg caaaaattat gcttacttag
    28861 gtctcccggc acacttctta atctttgatg aatacgtcgc tttcatggaa atgctgggaa
    28921 caaaagaaaa caccgcagtt atgaataagc tgaaacagat tgtcatgtta ggtcgtcaag
    28981 ctggcttctt tctaatactg gcttgtcaac gtccagacgc aaaatattta ggcgacggaa
    29041 tccgtgatca
//
LOCUS       DACEMK010000028        28202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEMK010000028 DACEMK010000000
VERSION     DACEMK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.028"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(208..789)
                     /locus_tag="IW715_001045"
     CDS             complement(208..789)
                     /locus_tag="IW715_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1021663.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(972..1598)
                     /locus_tag="IW715_001046"
     CDS             complement(972..1598)
                     /locus_tag="IW715_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1021664.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1620..2951)
                     /locus_tag="IW715_001047"
     CDS             complement(1620..2951)
                     /locus_tag="IW715_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1021665.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3106..3507)
                     /locus_tag="IW715_001048"
     CDS             complement(3106..3507)
                     /locus_tag="IW715_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1021666.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3593..3799)
                     /locus_tag="IW715_001049"
     CDS             complement(3593..3799)
                     /locus_tag="IW715_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021667.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3894..5396)
                     /locus_tag="IW715_001050"
     CDS             complement(3894..5396)
                     /locus_tag="IW715_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1021668.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5414..5869)
                     /gene="argR"
                     /locus_tag="IW715_001051"
     CDS             complement(5414..5869)
                     /gene="argR"
                     /locus_tag="IW715_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1021669.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5967..6584)
                     /locus_tag="IW715_001052"
     CDS             complement(5967..6584)
                     /locus_tag="IW715_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021670.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6805..8178
                     /locus_tag="IW715_001053"
     CDS             6805..8178
                     /locus_tag="IW715_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1021671.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8253..9365
                     /locus_tag="IW715_001054"
     CDS             8253..9365
                     /locus_tag="IW715_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1021672.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9437..11074)
                     /locus_tag="IW715_001055"
     CDS             complement(9437..11074)
                     /locus_tag="IW715_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1021673.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11242..11850)
                     /locus_tag="IW715_001056"
     CDS             complement(11242..11850)
                     /locus_tag="IW715_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1021674.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12234..13385)
                     /gene="queG"
                     /locus_tag="IW715_001057"
     CDS             complement(12234..13385)
                     /gene="queG"
                     /locus_tag="IW715_001057"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1021675.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13404..14156)
                     /locus_tag="IW715_001058"
     CDS             complement(13404..14156)
                     /locus_tag="IW715_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021676.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14297..15247)
                     /locus_tag="IW715_001059"
     CDS             complement(14297..15247)
                     /locus_tag="IW715_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021677.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15248..16087)
                     /locus_tag="IW715_001060"
     CDS             complement(15248..16087)
                     /locus_tag="IW715_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1021678.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(16084..16830)
                     /locus_tag="IW715_001061"
     CDS             complement(16084..16830)
                     /locus_tag="IW715_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021679.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            17068..18438
                     /locus_tag="IW715_001062"
     CDS             17068..18438
                     /locus_tag="IW715_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1021680.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18493..19389)
                     /locus_tag="IW715_001063"
     CDS             complement(18493..19389)
                     /locus_tag="IW715_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1021681.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19670..20347)
                     /locus_tag="IW715_001064"
     CDS             complement(19670..20347)
                     /locus_tag="IW715_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1021682.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20438..21343)
                     /locus_tag="IW715_001065"
     CDS             complement(20438..21343)
                     /locus_tag="IW715_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1021683.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            21518..22972
                     /locus_tag="IW715_001066"
     CDS             21518..22972
                     /locus_tag="IW715_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1021684.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22988..24019
                     /locus_tag="IW715_001067"
     CDS             22988..24019
                     /locus_tag="IW715_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1021685.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            24026..25213
                     /locus_tag="IW715_001068"
     CDS             24026..25213
                     /locus_tag="IW715_001068"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1021686.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25369..26751
                     /locus_tag="IW715_001069"
     CDS             25369..26751
                     /locus_tag="IW715_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1021687.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26723..27109
                     /locus_tag="IW715_001070"
     CDS             26723..27109
                     /locus_tag="IW715_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021688.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27309..27977)
                     /locus_tag="IW715_001071"
     CDS             complement(27309..27977)
                     /locus_tag="IW715_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1021689.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7861 a         5769 c         5176 g         9396 t
ORIGIN      
        1 aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaag
       61 cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg aaaaataagc
      121 cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
      181 gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta acaatctttc
      241 tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt tcaacaataa
      301 gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt cttcatcgga
      361 tagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc attgattgac
      421 taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa taatcaaatt
      481 acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa tccagtccaa
      541 catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt cgaacaattc
      601 tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt tttttgaaga
      661 aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat ctcggattga
      721 cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga tcttttcctt
      781 tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac agtaaattca
      841 tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac acagccctca
      901 ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg aggacaaaag
      961 caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac ttgatctgca
     1021 ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc accaattgca
     1081 tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg ggaatcgcta
     1141 cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt tctcaattgg
     1201 agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat gatcgtattt
     1261 tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc ttccactgat
     1321 tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc tcctgcaccg
     1381 ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa gagtttgtat
     1441 cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt gtatttttct
     1501 aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc gacttgcggg
     1561 tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc attcgttatt
     1621 tattttaata catcatttag agcttcactc attgtcggat gagaatagat ttgatctctc
     1681 aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat gacttcatga
     1741 gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc gactaaaact
     1801 ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct tgtcatatac
     1861 ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa acctacattg
     1921 gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt cgttgttcgt
     1981 ttattctcgc ccttcaactg atccataatg attcgataat catctagaga gatataagtg
     2041 aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc tgtacgaagg
     2101 tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt ttctaatcca
     2161 agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc ttggattgtt
     2221 acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc tgctacttga
     2281 tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc taaaatcatt
     2341 tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag aacgacaact
     2401 ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc agccccgatg
     2461 atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc aatcaagtag
     2521 cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt attgatgaaa
     2581 atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat ttgatggtta
     2641 gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct gatcatatga
     2701 taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc ctctgtaaag
     2761 tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc gatgttgata
     2821 catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc accttttgaa
     2881 gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat attttgatat
     2941 ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa aatatgataa
     3001 ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa
     3061 gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa caatcgttac
     3121 ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat gcgcatcttc
     3181 tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta atactacacc
     3241 gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta ctgtggactc
     3301 tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac aagaagcata
     3361 acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg gaggagtgat
     3421 tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc catggttgat
     3481 cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc atttgctact
     3541 tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct gcttattcga
     3601 ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta ctcgtgaaca
     3661 acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct tttatatgat
     3721 tgattttaag atacatatat accagaaagt tgaccccata aaccaatact gttaaaaaaa
     3781 taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag cactcgattt
     3841 aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact ggtttattct
     3901 gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt tactaacgcc
     3961 aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg ctcatgtaag
     4021 cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata tcgaatacct
     4081 tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg cttcatttct
     4141 tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa tcggatttct
     4201 tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat accttttctt
     4261 tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc atccagaaaa
     4321 tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt atctttccca
     4381 agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat ataacgaact
     4441 tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg ggaattcgtc
     4501 acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc gatcaaatta
     4561 tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac atctgtatcg
     4621 aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact ttccatcgct
     4681 tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc caaaaattgg
     4741 gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa tccaccagca
     4801 ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc tagtgttcca
     4861 atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag gtctgctttt
     4921 tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat aaaagcaata
     4981 tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata ataataggga
     5041 agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa ttgagtggac
     5101 ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc ttgttctttc
     5161 agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc atgaccaatc
     5221 ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat ccagtttgct
     5281 agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata tccttcaact
     5341 ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc tttcatataa
     5401 aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt
     5461 cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg acaacttcag
     5521 ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt tcagtcaata
     5581 cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt gctgtacgca
     5641 aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt actattttgt
     5701 tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag
     5761 tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt
     5821 gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata ttagacacct
     5881 ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt attttttact
     5941 tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg caaaatcgtc
     6001 ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt gtgttcttta
     6061 gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa cgttgctggt
     6121 tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc catctttttg
     6181 aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat ggcttcttgt
     6241 gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga aaaatgttct
     6301 tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc ttccagtgga
     6361 tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga catgcgtatt
     6421 gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg attcgggtcg
     6481 ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg cgggcttttt
     6541 ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct tcctccgcga
     6601 aattccattt agttatttat tatcatacct ctttttgagt gacttggcta atcgtgttga
     6661 cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag ataacacgtt
     6721 tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc tcttttttta
     6781 cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt agtacctgca
     6841 gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc catgaatgta
     6901 actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca atgggtaaca
     6961 acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt gaatcgcaat
     7021 tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg tataatgatc
     7081 gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca aggtgcagcg
     7141 acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc cctcaatcga
     7201 cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc aattggcccc
     7261 acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct gtttcttgtg
     7321 cccgttttga ttctttcttt gattatcggc tggtttgcta ttcccgagat ggcagtacaa
     7381 aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt tagtggaagt
     7441 ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat catcggcggt
     7501 attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact gatccgactg
     7561 tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt ttatcagttt
     7621 ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca aggaagtagt
     7681 ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct
     7741 ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc tgggctgatt
     7801 ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc agtgggtgta
     7861 ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag caatatgatg
     7921 accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc aatgttcaat
     7981 accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat tatcaattac
     8041 gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc aaaagcagca
     8101 ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta ttatctgttc
     8161 tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt ttttttgtgt
     8221 gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt atcggtggcg
     8281 ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat gttgctttat
     8341 ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc ccttggttgt
     8401 cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct tttttcccta
     8461 aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg acacttttgc
     8521 ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga aaagaagaag
     8581 cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa gcacttccgt
     8641 tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat
     8701 atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc caggcaaaag
     8761 tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga gaatacacag
     8821 ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa gaattaggct
     8881 atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca tcttataaga
     8941 tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt cctttcatgg
     9001 atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat ttggctccta
     9061 ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact gatccagatc
     9121 agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg tcagattttt
     9181 ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc ggtctaggct
     9241 cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc ttaaatactg
     9301 gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg aaaaatccat
     9361 catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg gattacttct
     9421 tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt tcttttgctt
     9481 catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga atatcaagtc
     9541 gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa tctggtatag
     9601 gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt tgttgcacga
     9661 ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg aaatatccag
     9721 tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt
     9781 cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact agctcatcta
     9841 gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa
     9901 aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa tcatctttca
     9961 tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg ggtgtcgcat
    10021 aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact aggacttttt
    10081 ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa gaaatggccg
    10141 gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag gcagtagcaa
    10201 aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt
    10261 ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca aatacagcca
    10321 tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag gcaataaagc
    10381 cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat tctccaaagt
    10441 aacgtaatcc tagcacaacc gctgtattat ggagagcatc aaacaaaatg agtccatttg
    10501 gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc tcatcaaagg
    10561 aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat
    10621 aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc acagaaaatt
    10681 cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga ctttgataca
    10741 cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc
    10801 gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt gtcctaacat
    10861 cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg atcgttcctg
    10921 gactcccttt tgctagttga taagctgtct tcctatcaaa aacttcatga acattattac
    10981 cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt ccacttgtga
    11041 tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa
    11101 aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg ctgtgggaag
    11161 caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt ttctcggttt
    11221 catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg ttttagcagc
    11281 atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt gattggcagc
    11341 tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt aacgtccgtt
    11401 agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg cagagcttga
    11461 agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt caacaggtgc
    11521 aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt ctgttgtttt
    11581 gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt caccaacata
    11641 aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc
    11701 tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg tataaacttc
    11761 atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag caaaagttgt
    11821 acctaaaaca attttcttaa atgaattcat attaaactcc tttttttctc cgttttttat
    11881 tctttcgacg ctattaactt tatcatttag aaatatttca taggtaaaag aaacatgaca
    11941 tttaatgaca aaataacgaa ttatttaata tttgtaacat ttaaacaaat atttttttga
    12001 tacaaaacca taaaagcctt gttgtataag caaagtaatc gtttttctat gtttttttat
    12061 ttttgaatga aaaacgcaga ttctgtaaca ttcactgcaa taatccataa aaaaaagcaa
    12121 aaaaattttt gatttttatc aaattctctt aaaaaaatgt aaaaaataca aaaaaaccag
    12181 taattgttac aacaattact ggacaaaaac tttgaataat gttaactatt tttttatttt
    12241 aactttctcc gcaataattt ctgggcatgt tccaattcgt ttataacgac ttcttctgtt
    12301 tctttagcta atatttcatc taatacatca agccattttt ccggttcttt tgttcctaga
    12361 cgtccaatag cccaagctgc tgttcctctg atgacagggc ggacatctgt taaacattct
    12421 gaaatctcag gcaatgccgt tcgatcacct agattagcta aagcaatcaa tgcgtttctc
    12481 tgcaaaggtt tcttcccacg ccatgaacca gctaaatgcc caaattgctg tttgaagtct
    12541 ttattagaca tcgaaagcat tggctttaat tttggatata tttcgtctac ttttggttcc
    12601 atctcttcat gaaaatgaaa atcttttcct tgattgtaag ggcagactaa ctggcagata
    12661 tcacatccat aaatgacatt tcgcattttt ttacgatatt cttccggcat catccctttt
    12721 gtctgagttt gataagacaa acatcgctgg gcattcattc ttccgtcacc aagcaatgct
    12781 tttgtcggac aagcagtcac gcagcgcata cagtcaccgc aaccaaattc tcctggttta
    12841 tcaggctcaa atacaatatt agtaacgatc tcaccaagat agacaaatga accgaactct
    12901 tcagtgatca acaatccgtt tcgtccaata aatcctaaac cagctcgctg tgctacagcg
    12961 acatcgatca gttctccagt atctacttgc ggagctagac gccattcctc ttctttttgc
    13021 catttttctg ctcttgtttg gatgaattcg atcaatcggt tcaaacggtc tctgagtata
    13081 tcatggtaat cgatccccca agaagcacga gcaaactgcc cgcgtttttc atccctaggc
    13141 acttcttcat ggatcctagt aggatacgca agtgcaatgg aaatgatcga tttcggctga
    13201 tcaaatgtca tttctggata aagacgctca tcgatatttg gatgttcaaa gccagaagta
    13261 tggccagctg ctttttgttc ttccaacgat tcttttaatg aatcaaaagg ttccgccgtg
    13321 gtgaacccga ttttgtcaat tcctaatcgt ttgctttctt gtatgatttc ttcttttaat
    13381 gtcatggtct tcctcgcttg ctgttaacgg ctgtataacc cataatatag tttttctttt
    13441 ttcagctgga caaactggat ctgctgatcg gcaatcttct tttcttccat ccattttttt
    13501 cttgcagcaa atacttccca ataatttgtt gaaattaatt ttcttttgtt atctaatgca
    13561 ttaaaaaaat tcaatgaaac tggttttgta cttaacagtt catggagcct tacctcttcc
    13621 catggcgaaa actcagacag atcgcgggaa tacaatactt cttcatagag atttttttgc
    13681 tgtgtgtacg tgaatgtttg cgggattttc ccaggcctag caatttctac tagacgatta
    13741 taaaacatat tcattaattt gctgttggca ataaattgac ttttataata ggccgactgg
    13801 atctttttga ttactccttc tgctttgatg actgaaaata atgcaggcca tgctttgacg
    13861 gttatttcgt cgttttgttt aactaatacg ccaggtgtga agccgactgg agaaaggaac
    13921 acaaaataac cgataccggt attttcatct aaaggctgaa attcatcaaa caaccatttg
    13981 cctatgtaca gctcttcatc tggttctgtt gtctcatttt gatttgctat gaataattta
    14041 gctaattctg cttcaagaaa ttgaaggtct tgatccttgt cggtattttc ctgctggctg
    14101 atcaatcgaa agacttcttc tttaatcgta tatttatctt tacgtgtaaa attcatagcc
    14161 acaccttcct tccttactct aactataacg gaatttcttc aaaaaaggta tctgtacgac
    14221 gaaaaacagc ataatttttt ttcatatctc ttataaaaaa tgcaaggtct gctcctcaag
    14281 ctagaccttg cacttttcag ctttcttttg tttgtgacaa accttcatgg atcgtttcaa
    14341 tattccattt catcatctga tagtaagaat ctccttgttc tccttctttt gctatggaat
    14401 cagtgaacag tttatcatag attttcaaac ccgtttcttt tgctacccgt tccatgcttc
    14461 ttgagtctac actggtttcc acgaataaaa ccggtacttc ttttgctctt atctgatcaa
    14521 tgatcgcttt catttgatca ggcgtacctt gactttctgt attgatctcc catatataag
    14581 ctgctggcaa atcatatgct cgtgaaaaat acttgaatgc cccttcactt gttactaata
    14641 attttttgtt ctcttcgata gaagcaaaac tttccttcgc ttgtgtatct aattctttta
    14701 gtttttccac atatgcttct gcattttttt tatagagtgt cgcattttct gcatcttttt
    14761 cagagaatat acgtgcgatt tcctccacat attttattcc gttagatagg tctagccaag
    14821 catggggatc tgcttttgta tgttcttcac cgctagttaa ataaagaggt tctacatttt
    14881 tgctaactgc aaaataatct tttccttctt cttttttagc cgtttccatc aagttatcga
    14941 accagctgtt acctgtttca agattcaaac cgttgtataa aataacatct gcatcgcttg
    15001 cctttttgat gtcttctggt agtacttcat attcatgcgg atctgttccg actggtacga
    15061 tactgtggat atctatttga tctgttccga cctcttttgc catgtccgcc aagatcgaat
    15121 tggtagccac gactgacaac ttttctttac ttccttcatc cgcttggtca gaagcctgct
    15181 ttccgcaacc ggctaatacc aagaaactaa ctgccaagat taaaaagagt gatttttttg
    15241 ttttcatcta tctgcttcct ttctgaaata aaattccttt tgttggagaa aataaaaaag
    15301 caaggataaa aaatgcggta catgctaaca cgatcgtagc tcctgatggc caattgtaac
    15361 tataactgaa aaacacaccg acaactgcac ttagcatacc gattcctgca gctgtaatga
    15421 tcattttcag taaatggttc gttagcaagt aagccgtggc ggctggtgtg atcaacatgg
    15481 caatcaccaa gattgttcct actgtctgta aactgactac agccactaat gtcaaaaaga
    15541 acatcaaaag ataatggatc agcgatgtgt tcagcccata agcttttgcc attgtcggat
    15601 cgaatgatgt gatcttcaat tctttataaa agaaaaagac aaatatcaag acgattccac
    15661 taacgagtgc tgtcagcaac aaatcgcttt ccctaacagc taagacattc ccaaataaaa
    15721 tatgatacaa atctgtcgca ctctgggcat atgaaatcaa gataatccct aatgcaaaaa
    15781 aggagctaaa gacaattcca atcgctgtat catttttcaa tcggctccgc tgagtcacga
    15841 aaccaatcac tgctgctgtt agcatcccaa aagcagtggc tccaaagatg taactaaaac
    15901 caaacatata agaaatggct acacctggaa gaactgcgtg agagatagcg tctcccatca
    15961 aagacatccc tcgtaaaata ataaaagaac cgatgactcc agaagcaagt cctacaagca
    16021 tggaagtgac caaagcgtat tgcaggaatt gataattcat taatccatca ataaaggatt
    16081 ggatcatttt ttctctcctc cttttactaa atgtctgatg atctcaccat aggccaattg
    16141 aaggttcttc gatgtaaaga cttcttgtac tggaccggaa gcgatcagct gtttatttaa
    16201 aagaataacc tcatcgaaat attcttctac tttatgaaga tcatgatgga cgatcaaaat
    16261 cgtttttcct gaattcttca attcctgcaa gatgtcaaag atctttcgtt cacttaacgc
    16321 atcaatccct acgaatggtt catctaaaaa gatccattct gctccttggg ctagagctct
    16381 tgcaataaaa actctctgaa gctgtccacc cgatagttcg ctgatctgtc ttttggcata
    16441 ttcttccatc cctacttttt tcaaagcttg tcttgcacgt tctttttctt ttttcccagg
    16501 tctttgtcca attcgtaaag atggatatgt tcctaaaagt acaacgccta atacatcaat
    16561 tggaaaggaa agatccaatt cacttcgctg ttccacgtag gctatttttt tacggactga
    16621 gtttaccgac tgtccagaaa aggtgacttt accagtagta gctggtatca actctagcaa
    16681 agatttcata aatgttgatt ttcctgcacc gtttggtccg ataataccgg taattttttg
    16741 tggatttatc gtcaagttga tagttttcaa aacagttttc ccttgataag cggctgaaag
    16801 gtcttgaatt acaatggccg tacgttccaa atcttacact cctttttagg tttacctaac
    16861 tttttattta aatatagttt aactaaaatg gaaagcattt tcaagtagaa tgatgaaaaa
    16921 gtttttttcg aattttcctc gcattttatg aaaattttct gtagaatcac atcaagagtt
    16981 caataaggct aacctaataa tgaaaaacat gtgttgaaac gtttgattat tttttcttgt
    17041 tgactcaaca acgaaaggag tttttgtatg gaagaagaaa agttaactcg aagtcttagt
    17101 gcccgtcata tccaaatgat cgcactaggt ggaacgatcg gtgtcggtct gtttatgggt
    17161 gcctcctcaa ccatacgttg gacagggccc tcagtgatgg gagcctatgc gattgcagga
    17221 ttctttttgt atttgattat gcgtgcttta ggcgaaatgc tttacgttga tccttcgact
    17281 ggttcttttg caaattacgc gagcgagtac atccatcctg tggctggcta cttgaccgct
    17341 tggagtaata tttttcaata tattgtcgta ggaataagtg aagtgattgc tgtaggatct
    17401 tatatgaatt attggtggcc tgatttgccg gcaattattc ccggaatcat tgtcgtcttg
    17461 tttcttatgc tggcaaatct tatttctgtc aaggcgtttg gcgaattaga attttggttt
    17521 tcgatcatca aagtcataac gatcattttg atgatcattg caggtcttgg ggtaatctta
    17581 tttggatttg gtaatcatgg tcaggcagta gggatttcaa atctttggaa aaatggtgga
    17641 ttcttcactg gaggcgtcaa aggatttttc tttgcattat cgatcgttgt tgcttcctat
    17701 caaggaatcg aactgatcgg aatgactgct ggtgaagcag agaatcctaa aaagacgatc
    17761 attgaagctg tccagtccac tatcggtcga atcttgattt tttatattgg tgctattttt
    17821 atcattgtga gtatctatcc gtggaatcaa ttgagtgaga ttggttcacc ttttgtacaa
    17881 actttttcta aagtcggcat tacatttgct gcaggactga ttaattttgt cgtgattaca
    17941 gcggcattat caggttgcaa ttcagggatt ttcagcgcaa gtcgtatggt ctatacactt
    18001 gccataaatg gaaagatgtc taaaaaattt ctgaaactat ctcgtcacgg tgcgcctttt
    18061 tatcctgtcg tagcaatctc aggtggaatc ttaattggtc tgatcttaaa ttatcttcta
    18121 ccaatgttca tcaaccagtc agaaaatatt ttcgttttcg tatacagctc aagtattttg
    18181 cctggtatgg ttccttgggt agtgattttg atcagcgaga ttcgcttcaa aaagaagcac
    18241 gcagataaaa tgcatgatca tccttttaaa atgccactat ctccttatac gaattatgtg
    18301 acacttgctt ttttagctat cgtattgatt ttcatgttta tcaatcctga aacgagaatc
    18361 tcattgatta ttggactcgt tttcttgctt tacatgacga tccattactt tgtacgcgaa
    18421 aaaagaaccg gaaaataaaa aaagaacgaa gcttcacctt agtggaagct tcgttctttt
    18481 ttattgcttt tatcaattca attttagcgt ctcaccggga gtcaggaaat aattctccat
    18541 gctcatccca ttcagcgaca agatcgtttc tactggtacg ccagttcgtt cggctatttg
    18601 atttggacct tctccttcca aaactacggt tgtcgcagcc tgtgaagtcg cgctgttggt
    18661 attagtttca gaggaatctg ttgtgtcttg tgttgttcga gcttgcattt cagaagcttc
    18721 taccgactct gctgtctcag aatcagaaga tgttgtgtct tgtgtgcttg ttgactctgc
    18781 ttgaagagag gcgctagtct gcaaataaat atcggaaatc ttagcgacat gattcgtata
    18841 attagccgat aattgattga tcaaagtatc taagtctaca ccgtctttat tttctgctgc
    18901 ctgatacgct gcacgtaatt ttctgatccc ttcgtcggag attgcttgaa gctcacgtgc
    18961 tttttggtta gcaactgctg ataaaccagc tattccctct tgatttccct gaatttctgt
    19021 ctggtattct tcaatcaatg caggcgtctt ttcttcgatc tttttcgaat aatcatttac
    19081 gattgcttga agttgctctg gcaaaccatt tgctgttttt tcttcagaag tttgtgtctc
    19141 ttttgtttta gttgatgact cacttgtttg tttcgtctct gttttctttg tcgttgtttc
    19201 cgttatggtc gtacttttcg cttctgacga attttttcca tgaaaatata gacccgcaac
    19261 tcctcctgct atgatcaaaa acacggcaac tgctgcccca atcatgatca tccgcttacg
    19321 agctgcctgt ttttgctgtc ttcttcgggt catccgaaca ttttctcccc actcattttc
    19381 atcatacatt tttttcacca ccaattttta tgtataatag cgttatcaaa cgcttcatat
    19441 tcttattgta gcaaataatt gtcgctgtgc acatttttat ctttgttatt tataagaaaa
    19501 tttcaccagt tgagatgatt ccaagattgt ccgtcgattt ctccttgcag ctgcattgtt
    19561 cgttcgtcta tctcagccat cagtttcttt atttcatata agagcgcacg gtcttgatat
    19621 tctgattttt cgcttaattc atctaattgt tggatcagcc aatcatttgt cataatgaag
    19681 ttcctttcat ttcttgcagt gtaaaggtca agtcttcgat ctgctctttt gcctcagtca
    19741 gtgcttctac gatatatgct tgtttttcag attcttcttc ctgcattttc tgattttcct
    19801 gtactctttg aataaaccag tcaatccgct gagtcagcga tggtttgcct tccaatgaga
    19861 gatactgctg ataagcaatt agcttctccc aacgtttttc ctgggataaa tacataaatt
    19921 gtgctaaaac gaatggcgga agataggtca tatggaattt atttgcgatt cgctgatacg
    19981 gcctcaaaaa ttcggaaatc gtatattctt cttttcctcc agcttggtat tctttttccg
    20041 ccacacctgt tgtcaatacg attcctagtt ctttcccttt taatacatgt gctgctcgtt
    20101 ccaagacatt atcttgccaa atttttaaat gagcaggaga actataccag taaaacggaa
    20161 actggaaaat gatccgatca tgctgggcca agagctgtag ttcagtctct atatctattt
    20221 ttccgtctgg ataacttgat tccaaatgat gccaagtagc tggtaaacca gcagaagctt
    20281 ctttgaaaaa ttgctgattt aaagattctt cgatcgttgg atggctaatg atgataagtg
    20341 tacgcatccc ttttcctcct ttttagtctt cgctttacag tgtagcataa ccgccacaag
    20401 tttcttttgc cgatgcttgt cattttaacc agactaatca ggttgtccaa gaaactgcgg
    20461 tttgtttttc caactgtttt ttcaaggtat ccaaagcaat attcttaaaa tttttgtttt
    20521 tcatacgttt acgatagttc gtagccgttt ctccggtact ctttttgaac attcggctaa
    20581 atacgactag actattgaac cctacttgat aggcaatact ttccacggat tcatccgtca
    20641 gagaaagaag aaacgcccct cgattgatcc gtaccccgat caaatattcc ttgatactgg
    20701 tacccatatg atttttgaat actcgcgaca aatggctccg attgaccgct aaatgctggg
    20761 cgatttcctc aacagacaat ttttcagcat aatgtgcctc gatgtatttg actgtttcaa
    20821 cagctaaaga ggaatgaacc ttttgtacac caccgctgac ctttccgcca tcttttaaaa
    20881 ggactgataa aaaacggtag gttaatgctg ttaactgtaa ttcattttcc aaggtttctt
    20941 ccgtatcttt catgcattct aaaataatgt cgatatattt ttgtatgtca gaagagacca
    21001 tcgtgtattg gtcttctctg aaaagcgact gccgaatgat ttcttctgca gcactcccac
    21061 caaatgaaag ccagcaatac atccacggat cttctccatc tgataagtaa aacgtcgagt
    21121 ctcctggacg aatcaagaac agatcacctt ttttcaattg gaattgttga tttttcacat
    21181 gataagatcc tttgccttcc atcacgacat gcaggatgta atcattgcga atagcaggac
    21241 caaaagaatg aaacggcatc gttttagaga aaccacaaaa atcaaaatac aattcttttt
    21301 ctacttgttg atgttccttt tttaaaaaaa tcgattgctc cacgtctatc cctccttact
    21361 cctttagtat aatgcaacaa aaagttaaat aaaagtcaca gaaatatatt aaaaaggaag
    21421 cgatttcttg ttaagtttaa gtcattaaaa gatctttgtt attcgttgta accggtttac
    21481 ttagtattat atattgaaca ggagaggaat acagattatg aaaagcaaaa tgatccaacg
    21541 tgtgtcctac gctttaggcg cttttggaca cgacacattc tatgttacat taagcactta
    21601 ttttatggta ttcgtgacaa gccagatgtt cgcaggaaca gataaggcaa cgaatgcaaa
    21661 aatgattgct acagttacaa gcttagttgt aggtatccgc ttagttgaaa ttatttttga
    21721 tcctttgatt ggtggcatca tcgataatac tcgtacaaaa tacggtaaat tcaaaccatg
    21781 gttagttatc ggcggcgttg ttagttccgt actcttggta gcaatcttta ccaatttctt
    21841 tggcttagct actcataata cgactttatt tatcatatta tttgcaatcg ttttcatctt
    21901 attggatagt ttttattcat ttaaggatat cgctttttgg tcaatgattc cagctttgag
    21961 tacggaaaca aaggaacgtg aaaaatttgc tacgtttgct cgtttcggct cttctctagg
    22021 tgcaaatggt actactctag tcgttgtccc tatcgttact ttctttacct atcttgttac
    22081 tcataaacat attgaaggcg cttcaggctg gttctggttc ggtgtgattg ctgctgttat
    22141 ttcaggcggt actgcgatta tcactgcctt gggcacaaaa gaagtcgata ctgcgatccg
    22201 tcagcaaaat gaaaaagctg ggatcaaaga tgtgttcaaa gcagttgctc aaaatgatca
    22261 gttgatgtgg cttgcccttg cttatatcgc ttatgcgatt gccaatgcag caacaactgc
    22321 cgtattattc tacttcttca aatatgtact tggccaacca actgaatttt ggatcgttgg
    22381 tctagttgct gccttacttg gtttagtagc tgttccacta ttcccaatgc ttacacgcgt
    22441 catctctcgt cgctacgttt accttggagg gatcgcaatg atgatttctt cttacttttt
    22501 ctttacgatc gcaggttcaa gtttgccaat cgttatcatc gggctagtat tgttctactt
    22561 ccctcaacaa ttgattttct tatccgcttt gatgacgatc acagactctg ttgaatatgg
    22621 tcaatggaaa aatggtatcc gtaacgaagc tgttactcta tcaattcgcc ctttactaga
    22681 taaaatcgct ggtgctttgt ctaacgggat cgtagggttt gtcgctatcg cttctgggat
    22741 gactggtaat gctactgctg ctgatatcac tgctcatggc gtaacgactt tcaaagctta
    22801 tgcattctat gctcctgctg ctttgatgat tttctccgct attatttttg cttggaagat
    22861 tacattgaca gagaaaaaac atgcacaaat cgttgagaaa ttggaaaaca aattggctcc
    22921 agctgataca atggaagatg aacttcacga tgcagtagtt ccttcccatt aagaaaggat
    22981 cttgaaaatg aagattacag aaaaagattt tggccaaggt tatcacttga taaccttgga
    23041 gaacaaaaac aagctagcac tttctatctc tgatttaggt gctagaatcg tcagtttaaa
    23101 atcaaatgat cgagaattag ttttgggatt tgacaccgct gaagaatata tcgaaaaaga
    23161 tccgtatatc ggtgcttcta tcggtcgcac cgcagggaga atcgaaaatg gccacttttc
    23221 tctcaatgga aaaacttacc aattagccac agatccaaaa actggacata atctgcatgg
    23281 tggtgctcct ggatttgaat taaaaaaatg gtcttatgtc attttgaatg gcgaaaatga
    23341 agcttctgtc atttttacta ctacaagtcc cgacggagaa catggttttc caggtacaat
    23401 ggatgtggaa atccgctata ctttgacaaa agataatatc tggcgagtga cctctcgcgg
    23461 caccagtgac caagatactt tattcaatcc gacaaatcat gtttacttca acttgacggg
    23521 agatgcttct cagtcaatcg atcaacatga gctttggctc aatagcgaag cttatgctcc
    23581 tttgcgaaca gatagtattc ctataggggt aaaagaaaat gctgcaggaa gtgctttcga
    23641 ttttcaaata cctaaaaaat tagcgtccgt ttttgctagt gatttggatc aaaaaaatct
    23701 tgttgacgga atcgatcatc catttttctt aaaagaaacc ggcttgggta aagaagccgc
    23761 tcgcttgact agccccgata aaagaatcca agtagatatt gctacagatg cttctagtgt
    23821 ggttattttc actgcaaatt tcggcacaga aacaccagaa atgcgcaacc gaaaactggc
    23881 acatcacggt ggcatcacat ttgagactca aacagctcca ggtgcagagc gcttctcagc
    23941 atttggttcg attcatttaa aagccggatc agtatttgaa acagtgacag aattcaaaat
    24001 caaaacaaga aaggaataac tcatcatgga aatcaaaaga agcttaaaca caaaatttgc
    24061 cgaaacattc ggacctaaaa aaactacaca atacttctca ccaggacgca tcaatttgat
    24121 tggtgaacac acagattaca atggaggcca tgtcttcccc gcttccatca cttatggaac
    24181 gcaaggtgta gcagcacctc gagacgataa taaaatatta gtttattcca caaactttga
    24241 cgatatcggt gtcattagtt ttactttaga tgagctagaa tatgataaaa aagcaaactg
    24301 ggcaaattac gtaaaaggaa tggtccttaa attaaaagaa gctggacact caatcgatca
    24361 tgggtttgag ttattagtgg aaggaacaat ccctaatggt gcaggacttt ccagtagcgc
    24421 ttcattagaa ttattagtag gtgttgtatt agaagatctg tttgatttaa aaacagatcg
    24481 cttggaacta gtccaaacag gtaaaaaagt agaaaatgaa ttcatcggtg tcaattcagg
    24541 tatcatggat cagtttgcca tcggcttcgg tgagctagat catgcaatct tattagatac
    24601 aaatacgctg aaatacgaaa tggttccagt gaaactagat ggttatgcaa tcgtcatcat
    24661 gaatacgaat aagcggcggg aattggcaga ttcaaaatac aatgaacgcc gtagcgaatg
    24721 cgaagaagcc ttggcacgtt tgcaaaaaac tttgcctatc tcttctttag gagacttaga
    24781 tgaagaagaa tttgaatcaa ctattgacca aatcggcgat gatacattga tacgcagagc
    24841 tcgccatgcc gtttctgaaa atcagcggac tttgaaggca aaagctgaat tagaagctgg
    24901 gaacttagaa gcatttggtc aactgttaaa tgattctcac cactctcttc gctatgatta
    24961 tgaagtaact gggatcgaat tagatacact agtagatgct gctcaaaaac aagaaggtgt
    25021 cttaggtgca cgaatgacag gagctggatt tggtggctgt gcgattgctc ttgtcaaaga
    25081 agaaaatata cctgccttca aaaacaatgt ctatgatgaa tacatgaaag ttgtcggcta
    25141 tgctccggaa ttttatgttg cacacattgg aaatgggaca acaaagatcg atccagaagc
    25201 tgttcatgtt tgataaaaaa atcaacgatc tcagctaaaa acaaatagat aaaaataacc
    25261 tctcagcaat ttccaaatgt gagccttttg ctcgtatttg aaaattgctg ttttttattg
    25321 acacggaaag agaacgtgtg ttctaattgt gttgaagagg tgattgttat gtgggcgaat
    25381 caaaatccgg tatttgttta tgaaaaagaa ccttcaagag atattttatg tattgactgc
    25441 aaatcttttt acgcctccgt agagtgcgtt gaaaaggatc ttgatcccct tacaactaaa
    25501 ttagtagtca tgtcctatcc gtccgattcg acagaaacac ggggtagcgg attaatcttg
    25561 gcttccagtc ctgcagcaaa aaaagcttac ggcatcacca atatcagccg tgccagagat
    25621 cttcccttcc cctatccaag tgatttatat attgttgccc caaggatggc ctattatatg
    25681 gaaaagaaca gagaaatcaa ttctatctat aaaaaatatg cagacgaagc gaaccatcat
    25741 gtctattccg tcgatgaaag cttccttgat gtaacaaata gcatggcact ctttggtaaa
    25801 acaaatgcgt acgacatggc taaactgatc cataaagatg tgtttgagca gaccggaatc
    25861 tacacgacta taggaattgg cgataacccg ctacttgcta aactagcctt agacaatggc
    25921 tcaaaagacg gaaagcaaat gtttgctgaa tggcgttatg aagatgtacc agaaaagtta
    25981 tggaaaatct cagatatcac tgacttttgg ggaatcggca aacgaacagc aaaacgtctg
    26041 aaacaaatgg gtatacattc tgtttatgat ttagctcacg cggattatta tcacttgaaa
    26101 agcaagctag gggtcatcgg tactcagctt tatgcccatg cttggggcat cgatcgaagt
    26161 tttttgggac aagaatatac accaaaatca aaaagtatcg gaaatagtca aatcctagat
    26221 agagattata caagacgaga agaaatcgag atcgttatca aagaaatggc cgaccaagtc
    26281 ggcactcgct tgagacgatc tgcagcaaaa acacaagtag tcagcttatg ggtaggatac
    26341 tctttaggct atcttgatcc aaatggcaag accgggtttc accagcaaat gaaaatcgaa
    26401 gcgacaaatg ctagcagaga tctagcagat gctttattga ccattttcca ccagcattac
    26461 cactaccagg atattcggcg tgtaggcgtg aactgttccg acctcgtata cgcgacaggt
    26521 ttacagctta acctttttga agatcctgaa aagcagataa atgaagcgaa agttgatttt
    26581 gttgtcgata cgattagaaa aaaatacggc tttcaatcaa tcatccatgc gcatagtctc
    26641 ttgcaaggag gacgcgccat cgcacgtagt tctctcgttg gcgggcatgc aggtggtatg
    26701 agtggattag aaggtggaaa agatgagacg gacagcaaaa aaattcattg actataacga
    26761 gtatcgagat cggccatttg gattaaaatg ggggactgct tttgcgatgg aagaactagt
    26821 caacggtatc aaaaaaaatg aagaagaagc acgcaaacag cctcttccta aacaacaaat
    26881 gacattgcaa gaaatcgata gtatactgac agaagccttt ttatctcgta aagcagtaat
    26941 cattcagctc aatttaaaag atgaatttgg aagactcttg gatgaggtcg aagggcactt
    27001 cattggtgaa gcctatgaag attactttat gattgaccat cagccgatat tgtgggaaga
    27061 cgtacggtat attgagttaa aggaaacaaa aaaatggttt gtgacatgag aggttgtgac
    27121 agaagtgatc aacttcaaaa aataagaaag gatttttcga aaattgcttc ttaaattttt
    27181 gtggaatttt ggcttatttt tcgaagaagc aacttctgga acaacgtata ttagatatca
    27241 gaggttgtga aaaagtcgct tagttttgag taacaagcca aggcgctgat ttcacaacct
    27301 ctgtctatct atccatctat tcggtaagat tcatgcatga cgtcttcttt atttagtatg
    27361 tagagccctt tttcttctga ccattcaaca taaatcagct gttttacatc tgcatagcct
    27421 tctttcttca agttctccat aagccatgct tcagtcaatt gattggactg aagcacttta
    27481 tgctgaattt gcccttcatc tacaagcagg atcgataaag ctttgtcttc atctgatttt
    27541 ttcaatacac tcatttgacc ggcattctct aggataacat gtttggcatt ttcaagaaaa
    27601 aagcattctt gctgtcttaa gattgctcgc aactgctcca tctcgatatg gttatttgtc
    27661 aactctgtca tgttgatgac gccgtctttg attagaacag atggctcgcc tttgacccaa
    27721 cgattgacct tttcattttt ttggacgata cgttcgatac cataaatcat tactgcccaa
    27781 agcgcaatgg caaataggac atgccctaca tggacattgt catcataggt tgattcttct
    27841 aagatcccac ctaaaacaag cgtatagact aaatcaaacg gtgtgatttc tgacatggat
    27901 ttcttcccaa gcaaacgtac cactaataat aaaccgatca acccgaacac cagtttcatt
    27961 gtaattgaaa aaatcatttt ttctccccct aactatctgt taattaaatt atatatgctt
    28021 tctcacgaac acgcatggaa tatacaagga agaaatgtat ccttttacta aaaaaaggac
    28081 tgtgacactg tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcctt
    28141 ttttttatgc tctaaaatga tatttttaca caaccgttac ggttgtttta cgaaattgga
    28201 cg
//
LOCUS       DACEMK010000029        28193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEMK010000029 DACEMK010000000
VERSION     DACEMK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.029"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..543)
                     /locus_tag="IW715_001072"
     CDS             complement(76..543)
                     /locus_tag="IW715_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1021690.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IW715_001073"
     CDS             complement(663..2201)
                     /locus_tag="IW715_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1021691.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IW715_001074"
     CDS             2281..2967
                     /locus_tag="IW715_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1021692.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IW715_001075"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IW715_001075"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1021693.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IW715_001076"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IW715_001076"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1021694.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IW715_001077"
     CDS             complement(4453..5583)
                     /locus_tag="IW715_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1021695.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IW715_001078"
     CDS             complement(5583..7766)
                     /locus_tag="IW715_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1021696.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IW715_001079"
     CDS             complement(7763..8788)
                     /locus_tag="IW715_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1021697.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IW715_001080"
     CDS             complement(8833..9660)
                     /locus_tag="IW715_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1021698.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IW715_001081"
     CDS             complement(9657..10724)
                     /locus_tag="IW715_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1021699.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IW715_001082"
     CDS             complement(10750..11586)
                     /locus_tag="IW715_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1021700.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IW715_001083"
     CDS             complement(11583..12452)
                     /locus_tag="IW715_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1021701.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IW715_001084"
     CDS             complement(12522..13805)
                     /locus_tag="IW715_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021702.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IW715_001085"
     CDS             complement(13819..15504)
                     /locus_tag="IW715_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1021703.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IW715_001086"
     CDS             15779..16804
                     /locus_tag="IW715_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1021704.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IW715_001087"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IW715_001087"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1021705.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IW715_001088"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IW715_001088"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1021706.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IW715_001089"
     CDS             25476..26444
                     /locus_tag="IW715_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021707.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IW715_001090"
     CDS             26462..27367
                     /locus_tag="IW715_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021708.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IW715_001091"
     CDS             27364..28170
                     /locus_tag="IW715_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1021709.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT         7786 a         6134 c         5317 g         8956 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctccct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctgatttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttgcttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaa
//
LOCUS       DACEMK010000030        27688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEMK010000030 DACEMK010000000
VERSION     DACEMK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.030"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            175..1914
                     /gene="yfcC"
                     /locus_tag="IW715_001092"
     CDS             175..1914
                     /gene="yfcC"
                     /locus_tag="IW715_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1021710.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2071..3255
                     /gene="iadA"
                     /locus_tag="IW715_001093"
     CDS             2071..3255
                     /gene="iadA"
                     /locus_tag="IW715_001093"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1021711.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3366..3569
                     /locus_tag="IW715_001094"
     CDS             3366..3569
                     /locus_tag="IW715_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1021712.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3670..4332)
                     /locus_tag="IW715_001095"
     CDS             complement(3670..4332)
                     /locus_tag="IW715_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021713.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4345..5847)
                     /locus_tag="IW715_001096"
     CDS             complement(4345..5847)
                     /locus_tag="IW715_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021714.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6395..8074
                     /locus_tag="IW715_001097"
     CDS             6395..8074
                     /locus_tag="IW715_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1021715.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8201..8680
                     /locus_tag="IW715_001098"
     CDS             8201..8680
                     /locus_tag="IW715_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1021716.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8899..9579
                     /locus_tag="IW715_001099"
     CDS             8899..9579
                     /locus_tag="IW715_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1021717.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9587..10918
                     /locus_tag="IW715_001100"
     CDS             9587..10918
                     /locus_tag="IW715_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1021718.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10933..11895
                     /locus_tag="IW715_001101"
     CDS             10933..11895
                     /locus_tag="IW715_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1021719.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12006..12344
                     /locus_tag="IW715_001102"
     CDS             12006..12344
                     /locus_tag="IW715_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1021720.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12423..13130
                     /locus_tag="IW715_001103"
     CDS             12423..13130
                     /locus_tag="IW715_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1021721.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13233..14714)
                     /locus_tag="IW715_001104"
     CDS             complement(13233..14714)
                     /locus_tag="IW715_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1021722.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15023..15559
                     /locus_tag="IW715_001105"
     CDS             15023..15559
                     /locus_tag="IW715_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1021723.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15637..17007
                     /locus_tag="IW715_001106"
     CDS             15637..17007
                     /locus_tag="IW715_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1021724.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17074..17409
                     /locus_tag="IW715_001107"
     CDS             17074..17409
                     /locus_tag="IW715_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021725.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17490..18347)
                     /locus_tag="IW715_001108"
     CDS             complement(17490..18347)
                     /locus_tag="IW715_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1021726.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18454..18969
                     /gene="tadA"
                     /locus_tag="IW715_001109"
     CDS             18454..18969
                     /gene="tadA"
                     /locus_tag="IW715_001109"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1021727.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18992..19076
                     /gene="ffs"
                     /locus_tag="IW715_001110"
     ncRNA           18992..19076
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW715_001110"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19286..20671
                     /locus_tag="IW715_001111"
     CDS             19286..20671
                     /locus_tag="IW715_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1021728.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20708..21385
                     /locus_tag="IW715_001112"
     CDS             20708..21385
                     /locus_tag="IW715_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1021729.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21393..22157
                     /locus_tag="IW715_001113"
     CDS             21393..22157
                     /locus_tag="IW715_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1021730.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22159..22818
                     /locus_tag="IW715_001114"
     CDS             22159..22818
                     /locus_tag="IW715_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1021731.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22848..23357)
                     /locus_tag="IW715_001115"
     CDS             complement(22848..23357)
                     /locus_tag="IW715_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1021732.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23469..24467)
                     /locus_tag="IW715_001116"
     CDS             complement(23469..24467)
                     /locus_tag="IW715_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1021733.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24668..25252
                     /locus_tag="IW715_001117"
     CDS             24668..25252
                     /locus_tag="IW715_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1021734.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25329..26504)
                     /locus_tag="IW715_001118"
     CDS             complement(25329..26504)
                     /locus_tag="IW715_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1021735.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26586..26966
                     /locus_tag="IW715_001119"
     CDS             26586..26966
                     /locus_tag="IW715_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1021736.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27040..27402
                     /locus_tag="IW715_001120"
     CDS             27040..27402
                     /locus_tag="IW715_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021737.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9000 a         4676 c         5775 g         8237 t
ORIGIN      
        1 aatataaatg gaaagagaaa aaaataatca tacataggag aaaaaaaaca aaatagaaaa
       61 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
      121 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
      181 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
      241 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
      301 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
      361 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
      421 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
      481 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
      541 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
      601 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
      661 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
      721 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
      781 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
      841 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
      901 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
      961 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
     1021 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     1081 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     1141 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     1201 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     1261 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     1321 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     1381 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     1441 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     1501 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     1561 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     1621 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     1681 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     1741 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     1801 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     1861 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     1921 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     1981 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     2041 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     2101 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     2161 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     2221 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     2281 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     2341 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     2401 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     2461 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     2521 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     2581 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     2641 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     2701 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     2761 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     2821 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     2881 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     2941 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     3001 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     3061 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     3121 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     3181 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     3241 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     3301 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     3361 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     3421 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     3481 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     3541 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     3601 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     3661 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     3721 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     3781 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     3841 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     3901 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     3961 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     4021 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     4081 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     4141 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     4201 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     4261 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     4321 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     4381 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     4441 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     4501 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     4561 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     4621 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     4681 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     4741 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     4801 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     4861 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     4921 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     4981 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     5041 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     5101 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     5161 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     5221 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     5281 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     5341 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     5401 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     5461 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     5521 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     5581 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     5641 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     5701 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     5761 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     5821 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     5881 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     5941 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     6001 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     6061 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     6121 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     6181 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     6241 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     6301 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     6361 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     6421 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     6481 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     6541 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     6601 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     6661 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     6721 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     6781 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     6841 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     6901 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     6961 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     7021 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     7081 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     7141 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     7201 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     7261 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     7321 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     7381 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     7441 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     7501 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     7561 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     7621 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     7681 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     7741 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
     7801 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
     7861 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
     7921 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
     7981 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
     8041 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
     8101 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
     8161 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
     8221 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
     8281 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
     8341 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
     8401 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
     8461 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
     8521 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
     8581 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
     8641 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
     8701 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
     8761 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
     8821 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
     8881 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
     8941 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
     9001 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
     9061 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
     9121 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
     9181 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
     9241 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
     9301 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
     9361 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
     9421 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
     9481 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
     9541 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
     9601 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
     9661 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
     9721 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
     9781 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
     9841 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
     9901 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
     9961 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
    10021 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    10081 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    10141 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    10201 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    10261 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    10321 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    10381 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    10441 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    10501 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    10561 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    10621 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    10681 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    10741 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    10801 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    10861 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    10921 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    10981 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    11041 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    11101 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    11161 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    11221 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    11281 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    11341 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    11401 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    11461 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    11521 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    11581 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    11641 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
    11701 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    11761 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    11821 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    11881 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    11941 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    12001 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    12061 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    12121 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    12181 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    12241 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    12301 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    12361 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    12421 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    12481 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    12541 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    12601 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    12661 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    12721 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    12781 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    12841 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    12901 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    12961 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    13021 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    13081 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    13141 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    13201 atttggaata agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa
    13261 ccttttttca tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg
    13321 tgcgttgtat ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta
    13381 atgatcaaat cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac
    13441 tgctgcaaat ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg
    13501 atgagtaact gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat
    13561 tgactgattt ggtttctttc cccatttttt gaaaaaattg tatcgatttt ttctcttaat
    13621 ttcttttcct gtataaaaga actagaatga ttggacgtgg cttctggaaa gcaaagtgta
    13681 gaaaaactac tgctggattc acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa
    13741 taaaaataaa ttggtatttt attttctaaa tggttgagag gaaactgttg agttaagtaa
    13801 gtcatcaatt gaccgacttg tcgacaaata aggtttttgc ttgtggaaaa taaaaaacca
    13861 atctcctgtt tttgtcgaaa agagatttga tttggaaaaa aacaatagaa aaaataatta
    13921 aagtaggctc tctctgtttc tgtctcttca caagggcgac tcactgaaaa agaagtaaag
    13981 tattctgaat aatcatgtgt atgctgcaat aagtccatca attcagtgat ttcagaagaa
    14041 gtttttggaa aatatgcctg ttgtttgttt cgtaatatga ataaagttaa cagcaagtag
    14101 atattttttt cttgttttga aattttttct atttgctcat ctgtactgtt catttttagt
    14161 gcatttaatt caagtgagga aaatggccaa tcgttacctc caaatgtatc tgaaagaaaa
    14221 aagtaaagat agagacggat agataactca tttccttcaa gagtacagca attttttaca
    14281 gataggatcc gtaaatggaa tttttttaag gaatgattga gctgttttgt cagtttttcg
    14341 agataggagg gcgatatata aagttgttca agtaaatatt tttttggtac agcagtttgt
    14401 ggataattga ttagtaacat caaatagtta aaacgattgg attcttttag gtagcataat
    14461 tttaacttag aaaaaacagc taacttttta gtttgatcgg tttctttggt ttggatctct
    14521 cctttatgat tagaataaat aaattcatca atcaataaaa attgcttgag tgatacattt
    14581 atttttcgga tcagttcttt gattgtccgt tttggaatat gtaagtcatt ggataattct
    14641 tgtaaagaac aattattttt ttgaatgacc atttgtagaa caagtaactc tttttttctt
    14701 gcttcaatta gcataaatac ctccttttta ttctgttttt gtatatagtt agacaaaaac
    14761 tgtgagtgga aagtcatttc agtctgtgtt attctcacag agtgtcgcct tcttttttat
    14821 ataacgttat ttaatttttt tgttaccttg tttattctat tgtttatttc ctagggtatt
    14881 ttgtcaagat attcttgttt ttaaggcgga aaaattgatt tttaatggat ataactaaat
    14941 atgttttgat aattatcaat aatattttta gaattattta tgttatttat ttatgggtag
    15001 atgaaaagag acggaggtaa gaatgatggg gagagtaaca aattatagta aagaatattt
    15061 gctgttcaaa agtatggtat acgtggaaga atatggaatg aagtcaatta ctgcaagaga
    15121 gttagcagag ttctgcggtt gttctacata cccgatttat acacatttca aatcaatcag
    15181 cgggttaaaa gagaagatat tagaagaaat cactgtttat tttgaaaact acttagctga
    15241 atgcagttcc gatgatgttt taagcattgt ctacttattg aaagattttt tttctatcca
    15301 tgaaagtatt cgtgaaatca ttgcaaaatc tgatttagac atggattcgc tatttaagcg
    15361 gccatttttc tcatatgtca aaggtcacac gaagatcaaa gaagaaaagt cgcttgaatt
    15421 ggtatggctt aatgcattgg gaagtttgcg ctcagatgat tcttctcaag attttcttga
    15481 aatgatgaac gctgtttggg ataaaaacga ggtgacggat gaatacttgg aaaatatctt
    15541 aaaggaacag tcaccttagg cagcaacatg aaaaagtaga tcagccacct ttatttttcg
    15601 taaaaaagat tgtaaaatgt ctaacgaaac attggaatga aggaggaagc gacaacgtta
    15661 ccacagcaat ttatcaaaaa atatcgtctt cttttaggcg aagaagcatc cgattttttt
    15721 tctgcgcttg aacaaggaag cgtgaaaaaa ggatttcggt ggaatccact aaaaccggca
    15781 ggattggaca tggtccaaac ttaccatagc gaagaattac aacccgctcc ctacagcaat
    15841 gagggatttc ttggcactgt caatggaaag tctttcctcc atcaagctgg gtatgaatat
    15901 agccaagaac caagtgcaat gatcgttgga acagctgctg cagcaaaacc cggtgaaaaa
    15961 gttttggatt tgtgtgcggc tcccggtggt aaatcaaccc aattagctgc tcaaatgaag
    16021 gggaaagggt tattagtgac gaatgagatc tttccgaaac gtgcgaagat tttatcagaa
    16081 aatatagaac gatggggagt ttcgaacgca atagtgacaa atcatgcacc cgcagaactt
    16141 gtccctcatt tttcgggctt ttttgatcgg atcgtggttg atgctccttg ttcaggagaa
    16201 ggaatgttcc gaaaagatcc aaatgcaata aaagagtgga cagaagaatc tccgctatac
    16261 tgtcaaaaac gtcagcaaga gattttgtct tcagcaataa aaatgctaaa aaataaagga
    16321 caactgattt attcgacttg tacgtttgca ccagaagaaa atgaagagat cattagctgg
    16381 cttgtagaaa attatccagt aacgattgag gagattcctt tgactcaatc cgttagttca
    16441 ggacgttctg aatggggaag cgtagctggt ttggaaaaaa caatacgtat atggcctcat
    16501 aaagatcagg gtgaaggaca ttttgttgca aagctgactt ttcatggaca aaatcagatg
    16561 cataaagaaa agaagacacg aaaaaaaagt aaagtgcaaa tgacaaaaga gcaggaaaaa
    16621 ctctggacgg agttttcaaa tgattttcat tatgaagcta ctggaagact tttagtgttc
    16681 aacgaccatc tttgggaagt tcctgagtta gccccttctc tcgatggttt aaaagtcgta
    16741 cgtacaggtt tgcatttagg agatttcaaa aagaatcgat ttgaacctag ctatgcatta
    16801 gcgcttgcta caaaaaagat agagaatata ccttgcttgc cgatcactca aaaggaatgg
    16861 caatcttaca cagcaggaga aacatttcaa cgtgatggga atcaaggatg ggttttgcta
    16921 gtattagata agatacctgt cggatttggg aaacaagtaa aaggaacagt aaagaacttt
    16981 ttccctaaag gattgagatt tcactgagtg gattagttcg tttatttttt tgtctatgct
    17041 atgatggaga tagataaaaa ggagctgata cttatgaaaa catcaacaaa agtaacaatc
    17101 ggattaagcg tagcagccgc tgccagtgta gcgacagcag tcgttgtatc gggaaaagta
    17161 atggaaaaga tccatcatat gactactcgg acgaaagtaa aaaaattcgt ctatgacaaa
    17221 tttgatggaa atgaaaaatt actagaaatc gtagatggtc tttcagatag tgacttggat
    17281 tctttgatgg gaatgttagc aaaaatcaaa tcaggaaaaa agaaaatctc tgtttatggt
    17341 gattcaatca aagattcaac cgaagatgta aaagaccggc taatgcattt tgttgaaaaa
    17401 atgatgtaag agaaactagc ttttgaacac tattcattcg aaataagggg ttgtgacaaa
    17461 attattgtca caaccccttt aatctatctt taattatgtt catgaagcaa taaaagatag
    17521 caaaacagta aatcgttggc tttttttaga ttgaaaccag actgttcttg aaatttttcg
    17581 attcgatatt ttagagtatt acgatgaaga aacagtgttt ttgcagcaga gctgacattg
    17641 cctaattcat tccataattc ttgaatgatc acttccattt cattttgttt gatcagttct
    17701 atgtaacttt tgatcaactt actatctttt acagtatctt ttgtatagta atgtaaagcc
    17761 acatcttgca tagaaaaagt acggctgtga gtataaagat tgttttgctc tgttaaaaaa
    17821 atttttcttt cttcagcaaa caaagggacg aggtcggtgc caggtgaatg gaaagaacca
    17881 acgaatacag acgttgtcgt atcaaaatca gcatctaaag atagaaaaat accgttgatt
    17941 tcagtagcat caagataatc acggtttatt tgttcgacta agagcgcatc tgtgtctgta
    18001 taaaaaaaga aatcagcaga atagcgaaat aattccaaaa tagtatcttg ccattctttt
    18061 tttaagaatt ctccttttga ttcgacgtga agctggatca aacgaaagct tttttcttga
    18121 acacaagaag catctttaaa aagatagtga taccatgggt gttttttttg atcgttgatt
    18181 acagggaata acgctttcaa aaggctggtt tcttgttgag ataatgaatc ttgtttgatc
    18241 cagataaaag catcatcaat tgcaaaagaa aggatatccg cttctgatgc tggagagttt
    18301 tttttctgtg cttgtgggta aagagcaagt aatttatcca ttttcatctt gttcacctct
    18361 tgtatttatt ttaccataga aaaaaaagtt agttacagcc tttttatcat atgctaaagt
    18421 aaaacaagaa aaaacaaagg cagggaaggg taattggtaa atacaaatct tgatgcagta
    18481 gaaaaagaaa aatggatgcg ccttgcattg gcagaagcaa agaaagcaga aatgcttcat
    18541 gaagttccga ttggagcagt ggttgtattg gatggaaaag tgattggaag aggatataac
    18601 cttagagaaa ccacgcaaga tgctactaca catgcagaaa tgcttgcaat caaggaagcc
    18661 tgtgaaaaag tgggaagctg gcgcttggaa gatgctgcac tttttgttac cttagagcct
    18721 tgtcctatgt gcagcggcgc gatgattttg tctcgtgtag cagaagttta tttcggcgcg
    18781 tatgatccaa aaggcggaac agccggtacc ttgatgaatc ttttggagga cgagcggttt
    18841 aaccaccaag catatgtaga aggtggcatt ttagaagacg aatgtgggat gattttgact
    18901 gactttttcc gaaagctccg ccagcgaaaa aagaaattaa aaaaagactg gtcaacgaat
    18961 gaaaaataag gtatactatt ctttgccgaa aggctaggac aatggtgggc ttgtgaagcg
    19021 tgtcaggtct ggaaggaagc agcactaagc aggttccgcc atgtgtctga ttataatgaa
    19081 acgacaacag actttcgggt ctgttttttt tatttttata gtactgtaaa aatgaatgtt
    19141 cctgattttt tagccagact gatttatcga atattgtact tatacaattt gcttcaaggc
    19201 aggaaaaact ctgaaataga gcttgtttca atggaagaaa aagctgccta tggtatgata
    19261 aagataaaga gaggcgagag ggtacatgga aaaaatagtg attttacata cgaatgatct
    19321 gcattctcat ttagaaaatt ggccaaggat cagaaggttt ttggatcaac gtaaacgaga
    19381 aaacgaaaag aaagaaaaaa cgacaacgat tgcagtagat ctaggtgatt ttgtggatcg
    19441 ttggcatcca ttgtcagaag caacgaatgg acaggaaaat atcttgctga tgaatgaagt
    19501 tggttatgat gcagcgacga taggaaacaa tgaaggcgta ggaaattcca aagaagagtt
    19561 gaatcattta tacgatcatg ctgagtttga tatcatcctg gataacttat tcgataaaaa
    19621 tacgctccag ccaccggcat ggacgaaacc atataaaatc attacaacga aacaccagac
    19681 gaaaatcgga ctaattgcat ttactgctcc atttccgctt acttataatc cgaatggctg
    19741 ggacatacgg aatccttatg atattttgcc agaattagtc gaagatttac gttcacaagt
    19801 ggatgtttta gttttgatga gtcatctcgg gattcaggat gatttccaaa ttgcaaaaga
    19861 aataccagca attgatgtta ttttaggatc gcacacccat catttattca gagaaggtca
    19921 cgtaatcaat gatgtccaaa tagcagccgc tggaaagtac gggcagtatg tcggtgaagt
    19981 ccatttaacc atcgatgaag agaaaaaaat ccttaagcat tcagccaaag ccatccctac
    20041 cgaaacaatg acagctttta cagaagatga acaagaaata aatagttatc tagaacgtgg
    20101 gcatgaatta ttaaaagaaa aagaagtagc agatttgcca ttttctctga gtctagatat
    20161 cttcaatgag cattcgttta tccaagtagc ctttgaggcg gtgaaagaac gaggaaagac
    20221 ggaagcagca atattaaaca gtggtctgtt tttaactgaa attccaaaag ggcgggtcaa
    20281 tcaggatcag cttcacactg cgttgccgca tccgatgcac ttgttaaatg tcacattggg
    20341 tggaaatgat ctgatccgct tagttttaga aatagaaaaa aatcgtaact ttttacgtaa
    20401 ctatcctatg aagggaatgg gatttcgcgg taagattttt gggcagatgg tctataatgg
    20461 aatcacatat gattctatta accatcaagt cttttggaag aacaagccaa tcgatctgaa
    20521 taaaaattat acttttacta ccgtagatca tttgatgttt gttccatttt ttccaacgat
    20581 tgagattgct ggaaaaaatg aatttttgtt ccctgaattt atccgaagtg tggtaggaga
    20641 ttacttgaaa gctcactacc caatcaaata aaaacaaggt ataataaaaa agataggtgg
    20701 tgaacgcatg tctgcaaaac aaaatgaaca gcagaacgtt gagcataaaa cagaaattgc
    20761 taagaaaaaa tcatcagctg aacaggaaac aacagtcacc gaagaactga cagctgcttt
    20821 ggaagaaatc gttgaggagc ctaaagcaga aaaagtcaaa ggcgagctag tgacactgtt
    20881 agatgacagt aattttttga taggtgaaag acattatcgg cttgtgtccg attatcgtga
    20941 aggatttaat gcagaaaagc ttggagaacg ttacagtgat gtactagccc gatatgatta
    21001 tatcgtagga gattggggtt atgaacaact acgcctaaaa ggatttttcc aagcggataa
    21061 tcgacgggcg catccagatc agcgaatcga ttccttggaa gactatcttt acgaatattg
    21121 caattttggt tgtgcgtatt ttgtcatcga gagaatcggc ggtaaaaaag aaaagacaac
    21181 tcagcgccga aaaaagaaaa aaaaccataa tcgtgcgtat atcgatgaga aaaaaggacc
    21241 agtcgcatct aatcgcaaaa aaccggttat taaaaatcga aaaacagagt cagcaaaaac
    21301 ctctgaaaaa gtaaaaaaac aagaacaacc taaacaagta gaaaaaacaa aaacagcatt
    21361 tacgatcaga aaaagggaag agtagtggat gaatggacta taaaggatat ttaattgatt
    21421 tagacggaac gatttatcgt gggacacaac ccataccggc cggtaaacga tttgtggaag
    21481 aattgcaaaa aagaaaatta cctttcttgt ttgttaccaa taacacgact aagtcaccag
    21541 aaacagtagc taatcgtcta gcagatgagt ttgacattca tgtagctcca gagacagtct
    21601 acacagctac tttggcaaca atcgatttta tgaaagcaga tggaaaaggg aaaaaagtct
    21661 atgttatcgg agaagcagga ttgattgact tgattttagc tgccggattt acatgggaag
    21721 aagaggctcc tgactatgta gtggtaggct tagataatta tctgacctat gaaaaagtag
    21781 ttaaagcaac acttgctatt caaaaaggtg ctactttcat tggaacgaac ccagataaga
    21841 atatacctac tgaacgcggc cttcttcctg gagcagggtc agtcatctct tttgtagaga
    21901 cagcaaccca aacgccgccg atctacatcg gaaaaccaga agccatcatt atggataaag
    21961 ccgttgaagt attagggctg caaaaagaag aagtaatcat ggttggagat aactatgaaa
    22021 cagatatcca agctgggata cgcaataata tagatacttt gctcgtttta tcaggtttta
    22081 caaaagaaga agatgtacct gggttgccta tcccagctac ctatgtgaaa caatcactgg
    22141 atgaatggag cttctaatat gaaaaattcg agatggcagt ggatggaata tgctggattg
    22201 ttcagcctat ttctgacact gatcagtctg gcagttggtg tgacgatcaa ctttcgacca
    22261 ctatacgtat ttgatatcgg acatttgcaa attttggatt atacttcttt agatcaagag
    22321 acgttactga aaaattttga tcacctaatg aattatctca ataatccctt caaaaccatc
    22381 ctcagcttgc cagatttccc agtttctgct agcggagccc atcactttta tgaagtgaaa
    22441 attttgtttt tagtggatta tgctgtgttt ttcatcactt tgatcccgag tattttgttt
    22501 atcaaatatt tgcaaaagaa tgatcggctt tggcggttga ttcgtccgtt tcaaatcggc
    22561 atgttactgc cagttgtttt cggttttttc atgatgatag gatttgatcg cttttttatc
    22621 ctttttcatg aaacgttttt caacaacgat gactggttat tcgatcccgt tactgatcca
    22681 atcatcaatg tacttccaga agaatttttc atgcatagct ttatcttatt ttttgtatta
    22741 ttagaattat tttttgctgt ttttcttttc ttaggaaaaa atagcttgaa acaaacaaaa
    22801 aagaaagaac tcgtctaaat agacgttttc tttctttttt tgttgaatta ttgagtaagg
    22861 cgacttcttt tttctggttc tttgtgtgct aatcgactgg cagcagcagc ggcgatcgct
    22921 ccgacgatat catctaagaa ggtatggaca tgaacgccat cgtggctgtt tagttctttt
    22981 aaaataccag gtttcacctt gtcgatataa ccataattcg taaagccgat cgtaccgtaa
    23041 acattcacga ttgacaaggc taggatctca tcgataccgt aaaggccttc gtcatcttcg
    23101 ataatatgtt gcaacggact catcaatttt ttcttttctg ctaatatatc taattggatt
    23161 ccagtgataa tggcgttatg tacttcacgc tttgttagca cgctgttgac actttcagta
    23221 caatccgcca aagtcaaatc agaaatatag tctttttgta aaaacatgac aagttgtgcg
    23281 atatcttcta cagttacgcc acgttccttc aatagttctc ttgccttttc ttctaaagtc
    23341 tcagttttga cgaccatatt attgctcccc ctttattttt tctttctcta tttcatgtta
    23401 acggaacatg aaaaaaatgc aagaaactgc tttcgggttt aacttgaaat gcaaagagat
    23461 ataaaaaatc aactaggacg aagattttta tcgtataagc ttgtgctgtg accaggctga
    23521 gttcgttcct ttggattaat atagtgtaaa gcgttgttca ctgcagtagg tgcttcaccg
    23581 agcccagtag caatcagttt cacttttcct tcataggtac agatgtcacc agctgcataa
    23641 acgcctggaa tagaagtaga catatctgat tttacagtaa tggcatttct ggtactatca
    23701 agtccccaag aagaaagatg ttctaaatta gaagagaatc cataattgac gatcagagaa
    23761 tcaatcatca aatcaatcgt ctcgtcactt ttcacttttt ttaaccggat atctgtcagt
    23821 tccccattat tgcctgataa tccatcgatg agatagggag taagcaagtt gacagatgaa
    23881 gatttcaagc gtgatacact atgttcgtgc gcacgaaatt ccgggcgacg atgaatcaag
    23941 taaacttcgc ttgcaatagg ctctaacatc aacgtccaat cgatagcaga atcaccacct
    24001 ccagcaatag cgactttttt tccagcatac ttcatcaaat cattcacgaa ataatccaat
    24061 ccgtggtttt caaatgattc agcattatct aaattcaatt ttcttggctg gaatgaacca
    24121 tttcctaaag ctaaaatgac tgctttggaa taatggatgc ctttatttgt agtaatctcg
    24181 ataacttcgt cttcacgagt aagcgagaga acttcttctt ttaaatggaa tgtatggtta
    24241 aaagtagtta attgtttttc taattgatcg attaattcac ttgcttttat tgctggatac
    24301 ccaggaatat cgtaaatata tttttcggga tacaaagttg ctaattgacc accaagttga
    24361 ggtagactgt caatgatttt tgttttagct tggcgcattc ctgcgtaaaa agcggcaaac
    24421 atccctacag gacctgcacc gatgattgta atgtcataga tttccataaa aattcctccc
    24481 ttttcacact atagcagaat ttaattgaga atgcttctca aaaatgtttg aattcgaaat
    24541 cgaaacggta agttatagaa tgtatctagt gatgaaaaat aaaagaaaac aaatctagta
    24601 tttttacgag aggttaggta ttatgttatt attattatga agtatgcaaa tattaggagg
    24661 aaacaatatg gcattcccac aactagattt aaaaagtgaa aaaggaccaa aagctgttat
    24721 caaaacaaac cgtggtgaca ttactgttca attattccct gaactagcac cgaagacggt
    24781 tcaaaatttc attgaacttt ccaaaaaagg ttattatgat ggcgtgattt ttcaccgcgt
    24841 tattcctgat ttcatgatcc aaggcggcga tccaactgga acaggaatgg gtggcgaaag
    24901 tatttatggc gaaagttttg aagatgaatt cagtcgtgaa ctgttcaacc ttcgcggagc
    24961 tttatctatg gcaaattcag gtccaaacac aaacggaagc caattcttta ttgtaaataa
    25021 cacaaatgtg ccagctaata tgcttggaca actagaaggt gctggattcc catcagaaat
    25081 cattgaagcg tataaaggtg gcggaactcc ttggctagat ttccgacata cagtctttgg
    25141 acatgtacta gaagggatgg acacagtaga tgaaatcgca aatgtacaac gtggtccaca
    25201 agaccgacct gtacatgatg tagtgattga aacaattgaa atttcagaat agtttggtat
    25261 ggtacggagt aattatgaaa aaggacgaaa cctagtaagg tctcgtcctt ttattttgaa
    25321 taaatgattt aatcgattgt ctctaaagca tacttgattc gattaagtcc ttccagtaaa
    25381 gtttcttctg gacaagcaat attcaagcgc atatgcccat gaccggaagg accaaacgtg
    25441 atgccaggat ttaatacgac ttttcctttc cgaacaagag tgttctccaa ttcttggtcg
    25501 tctgaaatat aggcggtaaa atctaaccac attaagtaag ttccttcagg gttcattatt
    25561 ctcactttcg gaagatactg ttcaaaaaac tgattaactg tttgaacatt tttttctaga
    25621 taaggaatca gttggcttaa ccattctcca cccgtctcat aagccgcttg ggttccaatc
    25681 agtccaaaag tgttgatttc gtgctgttgg ttcatcacta aatgtttttc aaaatcagct
    25741 ttcatctctg gatttttgat aaataccatt gaatttttga ttgcagctag attgaaagtc
    25801 ttggttgcgg aagtaaaagc aactagtaga tcatctaaat taggatcaat cgttagcatg
    25861 gaagtaaatg tatgatcgaa taaagttaaa tcttgatgga tctcgtcact aaacagaatc
    25921 acttgatgtt ttaaacacaa ttcgcttaac tgtttcaatt cttctttgct ccaaacacgc
    25981 cctccaggat tgtgaggatt gcataaaatc attgctttta cattgttttc ttcgattttt
    26041 ttctccatgt cagacaagtc catgatgaaa tgattatttt tttctagcaa agagcttcga
    26101 acgatgtgac gttgatttgt ttctataata gaggaaaatg gaggataaac tgggtcatga
    26161 atcaaaatag aatcgcctgg ttttgtaaaa gtttgtactg ctgcagctaa actggttaac
    26221 acaccacttg taaaaacgat attttctttc gtcaactcca cagcatggtg ttggcgttcc
    26281 caatgaatga tagcctgata tagtttatca gacagagtgg cataaccaaa gattccatgt
    26341 ttgatgtatt cactaaacgc tttagctacc ccaccgggag acaagaagtc catatctgct
    26401 acccacaaag gaagtaagtc atccatttga tatgtctcag caattgcgtc ccattttaca
    26461 ctttctgtat cttttcttga aatctgttta tcaaattcga tcatcttaag acctccagtc
    26521 attttcgtcg cttctttttt tgagctattt acagtataat gataaatgcg aagagaggga
    26581 agtagatgac ttataaaatt ggacaaataa ttgaaggaaa agtgacaggg atacagcctt
    26641 atggcgcatt cgtttctctt gataacgaga cgcaaggact tattcatgtt tcagaagtac
    26701 aatccggtta tacgaaaaat atccattcat tgttaaaagt cggacagcca gtaacagttc
    26761 agatcatcga cattgatgaa tattccaaaa aaatcagttt atctttaaga acgttagaaa
    26821 aaacaaatcc agctgttcca tatcgcagaa aaaggtattt cacgaataaa aatcgtaaga
    26881 tcggatttac cactattgcg gagcagttac ctatttggat agaagaagca ttatctgaac
    26941 tagaacggaa agaaaataag aaataaaaag tgtatttttt ctgaaaatgt gctagaattt
    27001 ttatgtaagc catagaacaa gttgatcgag gtggaaattt tggcaagcaa aagagttgct
    27061 ggtacaatta tgttgcattt ggaggatggt actaaaaaat ttttggtcca ttcaattgat
    27121 gacaagttgg aatttgcgtt agcggaactt tctgaaggaa agactggttt agcgaatata
    27181 cttaatttct taaaagaaat agttcatatc gatgttagca agattagttt gatggaattg
    27241 acaaatacac atattaaaga ggaaaatgtt ccgttgtttg tctttgaaac tgaacaaaag
    27301 aatcaaagag aagaattagg cgaaggatat atttgggaag aaccaggtca aatgcgctct
    27361 gttcttggaa cttacgaagt agagggagtg cctttcttct aaaatacaga ttaagcgaac
    27421 aaagaagttt gaattttttt caaacttctt tttttattgt ttggtccaaa aaatacaatg
    27481 aaatgttctg ctaaagatag ttttttagtg cattttattt tcaaaatatg ttgaaaacgg
    27541 ttgtataacg aataataata attgatcgaa aaatcatttc acaaagtaat gcttaattta
    27601 tagttgacca aggaagaaat acatgataaa tttaacagcg ttgattctga agtggatgtt
    27661 atatcacatt tctaaaaaat ttaaaaaa
//
LOCUS       DACEMK010000031        26814 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEMK010000031 DACEMK010000000
VERSION     DACEMK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.031"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1359
                     /locus_tag="IW715_001121"
     CDS             <1..1359
                     /locus_tag="IW715_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1021738.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
     gene            complement(1439..3427)
                     /gene="treP"
                     /locus_tag="IW715_001122"
     CDS             complement(1439..3427)
                     /gene="treP"
                     /locus_tag="IW715_001122"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1021739.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            3650..4363
                     /gene="treR"
                     /locus_tag="IW715_001123"
     CDS             3650..4363
                     /gene="treR"
                     /locus_tag="IW715_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1021740.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            4440..4886
                     /gene="msrB"
                     /locus_tag="IW715_001124"
     CDS             4440..4886
                     /gene="msrB"
                     /locus_tag="IW715_001124"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1021741.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            5056..5658
                     /gene="ruvA"
                     /locus_tag="IW715_001125"
     CDS             5056..5658
                     /gene="ruvA"
                     /locus_tag="IW715_001125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1021742.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            5671..6672
                     /gene="ruvB"
                     /locus_tag="IW715_001126"
     CDS             5671..6672
                     /gene="ruvB"
                     /locus_tag="IW715_001126"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1021743.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            6701..7279
                     /locus_tag="IW715_001127"
     CDS             6701..7279
                     /locus_tag="IW715_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1021744.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            7331..7813
                     /locus_tag="IW715_001128"
     CDS             7331..7813
                     /locus_tag="IW715_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1021745.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            7930..8304
                     /locus_tag="IW715_001129"
     CDS             7930..8304
                     /locus_tag="IW715_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021746.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(9104..10456)
                     /gene="brnQ"
                     /locus_tag="IW715_001130"
     CDS             complement(9104..10456)
                     /gene="brnQ"
                     /locus_tag="IW715_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1021747.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            10603..11193
                     /locus_tag="IW715_001131"
     CDS             10603..11193
                     /locus_tag="IW715_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1021748.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(11321..12670)
                     /locus_tag="IW715_001132"
     CDS             complement(11321..12670)
                     /locus_tag="IW715_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1021749.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(12838..13578)
                     /locus_tag="IW715_001133"
     CDS             complement(12838..13578)
                     /locus_tag="IW715_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021750.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(13591..15183)
                     /locus_tag="IW715_001134"
     CDS             complement(13591..15183)
                     /locus_tag="IW715_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1021751.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(15751..16047)
                     /locus_tag="IW715_001135"
     CDS             complement(15751..16047)
                     /locus_tag="IW715_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021752.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            16223..16948
                     /locus_tag="IW715_001136"
     CDS             16223..16948
                     /locus_tag="IW715_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1021753.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16941..17783
                     /locus_tag="IW715_001137"
     CDS             16941..17783
                     /locus_tag="IW715_001137"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1021754.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            17786..18307
                     /locus_tag="IW715_001138"
     CDS             17786..18307
                     /locus_tag="IW715_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1021755.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(18560..19123)
                     /locus_tag="IW715_001139"
     CDS             complement(18560..19123)
                     /locus_tag="IW715_001139"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1021756.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            19374..19643
                     /gene="rpsO"
                     /locus_tag="IW715_001140"
     CDS             19374..19643
                     /gene="rpsO"
                     /locus_tag="IW715_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1021757.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            19831..21957
                     /gene="pnp"
                     /locus_tag="IW715_001141"
     CDS             19831..21957
                     /gene="pnp"
                     /locus_tag="IW715_001141"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1021758.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            22467..22733
                     /locus_tag="IW715_001142"
     CDS             22467..22733
                     /locus_tag="IW715_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1021759.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            22877..24868
                     /locus_tag="IW715_001143"
     CDS             22877..24868
                     /locus_tag="IW715_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1021760.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            25222..26523
                     /locus_tag="IW715_001144"
     CDS             25222..26523
                     /locus_tag="IW715_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1021761.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8643 a         5068 c         5510 g         7593 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgtac aggtactgtt ctatggggcg cttactgact
     1441 aagattttac ttcagcttta ccgactgctt cgcccgggcg aacaagtcct gattggtcaa
     1501 gagaaagtcc tgcaactttt tcattattgg tcaaagcaac aataatgtca gttttcttgc
     1561 cagcttgttc tatttctggc aaatccattt cagcaatcag ggtctctggt gtgacaaggt
     1621 atccttcttt cacgaataca ttgaaaggaa caccctttag ttcaacagta tctaatccca
     1681 tgtgaacaag tacttccagc ccctcattag aaagaatacc aatggcatgt tttgtttcaa
     1741 agacactcgt cactttaccg tttactggtg catagacttt gccgtttgac ggttcgacag
     1801 cataaccgtc acccatcatt ttttgtgaga aaactgggtc atttacttca gttatttcct
     1861 tgatggtccc tgcagcgact gcgaataatg tttcttttgt tgcaactgcc gtaccagttg
     1921 ctgaaacagg ttcgaaagac tgtggcgttg ctgttgcgcc atttgctgtt tgaagttgta
     1981 catcagcagc attttcagga acagcaggat caatcttatt caaaattccc tgacgtctaa
     2041 agatgatcgt caagccaaat ggaatgatga cagcaatgat cattgcgatg atgaaaggta
     2101 cccatgtttc tgcttggata gctagtattc ctggcaaacc gcctactccg atcgcgttag
     2161 ctcgaacacc catcaagtta gcaaacatgc cagctaatcc agagccgacc attgcagcga
     2221 cgaaaggata gacgtatttc aaattgattc cgaacatagc aggttcagtt acgcctaagt
     2281 aacaagaaat cattgctgga atcgagattt gttcttcttt tttattccca cgatgtaaga
     2341 agatgatggc taagactgca gagccttggg cgatatttga taaagcaatc atcggccata
     2401 aattcgttga tccgaaatct gcaatcagct gcatatcgat ggcattggtc atatggtgga
     2461 gaccagtaat cactaaagga gcatacagga atccgaatac ggcactaaac aaccagctga
     2521 tagaagacgt caatccagta tttacgatat ttgagatcca gcttccgact gtccagccga
     2581 taggacctaa aataacgtgt gcagctaaaa cagttggtaa caatgaaaac aatggaacaa
     2641 aaatcatcga gatcgactga gggatatatt tgcggaagaa gatttctaga tacactaaca
     2701 taaaccctgc cagcatggct ggaataactt gcgcttgata accaatcatc tggacttgag
     2761 caaaaccaaa atcccaaaat ggaatatctg ctgcggctgt ggaagctaca ctataagcat
     2821 ttaataattg cggagaaact aacgtaattc ctaaaacgat tcctaatatt tgtgtagtac
     2881 ccatcttttt tgcaatactc caagtgatac ctactggaag aaaatggaaa attgcttcac
     2941 ccggtaacca caaaaaggca ttgacaccat tccaaaactt cgagtgttca acgattgttc
     3001 caccaagact ttcaaactgg ataccttcaa gtacatttct gaatccaagg atcaatccac
     3061 cgacaatgat tgcaggaatc agtggtgtaa agatttctgc taaaacggca atagcacgtt
     3121 gaacgggatg aaggttttgt tttgcagctg cttttccttg ttctttggaa accccttcaa
     3181 ctccagatac tgcaacgaag tcgttataaa aagtagaaac atcgtttcct atgatgactt
     3241 ggaactgtcc tgcattcgtg aacatccctt ttacgctagg tatatcttcg atcgcttttt
     3301 cgtccgcttt tcctggatca ttcaggacaa aacgcatacg agtcgcacaa tgactaactg
     3361 cagcaatatt ttcttttccg ccgatatcat gtaacagctt ctcggcatct gcttgatatt
     3421 ttgccatatc aataaacccc tttctcttaa cttgtctatg caagttgttt cttcatttac
     3481 tatacttttt ttcggagaga gttgcaagcg ttttattaaa aagtacgaga gaaaatgata
     3541 tcgttttaaa aagcttaaaa taagcttttc ttaaaaatag gaacattgaa atacaaaaag
     3601 agctgtagta tagtgaagaa atgatggaag aacgagtgag ggaaaaaaca tgaataagtt
     3661 caatgaaatt tttttagatt tggaacagaa aattttagcg aaagaatatc ctccgcattc
     3721 gcttttaccc agtgagaacc aattgatcaa aatttatggt gtttcacgag aaacaattag
     3781 aaaagcgctg aatcttttga ctacggaagg ttatattcaa aaaaaacaag gaaaaggttc
     3841 gatcgtactg gatcggaatc gcttcgactt tcctatttct ggattgacta gctataaaga
     3901 gctacaggag acccaacgta ttccaagtga aacgatcatc catacgttag aagaaacaga
     3961 agtgacaaaa gcattgaacg agatcactgg ctgggagatc ggtgcgcctg tttggcattt
     4021 gatacgcgaa agaaaaatca atggcgaagt agttatatta gacaccgatt atctattaaa
     4081 ggaaatcgtt cctattttaa ctccagcaca agcacaagga tcgatctatg aatattttga
     4141 aaatgagctt tctttgacga ttgcttacgc gcaaaaggag atcacagtag aagaagtaac
     4201 ggaaatcatg aaaacagcga tggatcttca cgatgacact tatgcagtag tagtcagaag
     4261 cctcgttcat ctggaagata cccgctgttt cgagtattct gagtcggttc accgattaga
     4321 taaattccgt ttcgttgagt ttgcccgacg ccgaaaagtg taaatagttc aaaagaaagc
     4381 aaattgttgg catctgtcca ctttggcgct atgataggat cacaaagggg gcatttttaa
     4441 tggataaacc aataagacca gaaaaagaag aactgaaaga aaagttatca cccatggctt
     4501 atgcagtcac acaagaaaat gcaacagaac gaccatttag cgggaaatat gatgattttt
     4561 acgaaaaggg gatctatgta gatatcgtca gcggtgaacc gttattcagc tctgcagaaa
     4621 aatatgatgc aggttgtggc tggccatcat tcagcaaacc aataacgaaa agacaagtgc
     4681 gcgaaaaagc cgatttttct catggcatgc atcgaataga agttcgaagc aaagaagcag
     4741 attctcatct cggacatgtg tttaccgatg gaccagtaga tcaaggtggt ctacgttact
     4801 gcatcaattc tgcctcactg aaattcattc catatgaaaa gatggatgaa ttaggttatg
     4861 gggaattcaa atcacttgta gaataatgga aatacacaaa gagcggtaca atacggggat
     4921 ttactcgatc gtccgctctt tttttattca agcgattgct atttgaaaga aacgctcccc
     4981 agactccttt tttcacaagg tgaaatgtgc tacaataaag ggaacgtatg tgtgcaaaag
     5041 aaagaggact agaatatgta tgaatatctg acaggtgtgg ttacctttat caacccctat
     5101 tacatagtag tagaaaccaa tggtatcggc taccagatcg cactaggaaa tccttaccgc
     5161 tacagtagca aactaaatca aaaaataaaa atttatgtcc atcaggtaat acgtgaagat
     5221 gcgcatcttc tgtatggatt tgattcgcta gaagaaaagc aactgttttt gcgattagta
     5281 agtgtttcag gaatcggtcc gaaaagtgcc cttgcaatca tggcaagcga agaccactcc
     5341 ggtttgattc aagcagtaga atccagtgat gtcacttatt tgacgaaatt tccaggagta
     5401 gggaaaaaga cagcacaaca aatgatcttg gatctaaaag gaaaattcgg tgagctgagt
     5461 atcgatacac cttttagtct atttgatgaa gcaaacacaa aagacgctac agctctatct
     5521 gaagcaatgg aagctctctc ggctcttggt tacagtgatc gagaaatcaa acgtgtagaa
     5581 aaacaattaa aagaagtaga taatcagaca acggatgagt atctacgcca agcactcaag
     5641 ctaatgatga aaaaataagg aggagaccaa atgacagatg aatacttgtt atcaccagaa
     5701 tctggcgaaa atgaactgag tttggaaaaa tctcttcgac cacagctgct ttctcaatat
     5761 atcggacaga gaaaagtcaa acaagaactt cagatttaca tcgaagctgc cagaaatcgg
     5821 gaagaacctt tagatcatgt tctgttgtac gggcctcctg gattaggaaa aacgacgatg
     5881 gccatggtca ttgctaatga gatgaccgta aatattcgta cgacgagtgg ccctgctatc
     5941 gaacgtccgg gtgacttagt agcaatcttg aatgaactgg aagcagggga tgtcctgttt
     6001 atcgatgaga tccatcgatt acctagagtg gtagaagaga tgctttattc agctatggaa
     6061 gatttctatg tagatatcat ggtaggacaa gggccaaccg cacatccggt tcatttccct
     6121 cttcctccct ttactttgat tggcgcgacg acacgggcag gtatgctttc tgcaccttta
     6181 cgagatcgat ttggcatcat ttctcatatg gaatattatg acgaacagga tctgcaggag
     6241 atcgttttac gttcttcgga tatttttcaa acagagatca ttgaagaagg ggcattggaa
     6301 attgctcgcc gatcacgagg aacgcctaga atcgctaatc gcttgctcaa acgggttcgt
     6361 gattttgcgc aagtccaagg agacgggaaa atcgatcgtc ctatagcgga tcaggcactt
     6421 actttgctac aggtcgatca ggctggattg gattatgttg accagaaatt gttgcgaaca
     6481 atgatcgagt tatatggcgg cggaccagta gggttaagta cgctttcagt aaacatagga
     6541 gaagagcgtg agacggtaga agatatgtat gaaccgtatc tgatccaaaa aggattttta
     6601 aagcgtacac ctagagggcg aattgctact gcttatgcct atgaacattt tggttacgac
     6661 tatcatatat gatttttgct tttagattgg aggaaaatga atgagtgata caacacgtac
     6721 gaaacaattg atcaaaaacg tattgattga tttgtgccaa acgcaatctt ttcgaaaaat
     6781 cagcgtccaa aaaatcgcaa atgaagcaaa aatcaatcga caaacatttt attatcattt
     6841 tacagataaa tacgatttgc ttcgttgggt ctattgtgag gatgcattgc attatttaga
     6901 tccacaatgt ctttcacttg ataattggga agagcaagca ttgaaagtgc tgaaagcaat
     6961 tgctgagaag aaagaatttt atgcaaatac tgtgctttct gacccagaaa ttttacaaaa
     7021 agagttttct caactgatcc aaaaatcatt tatcaagatt tttgaacaaa tggatgaaga
     7081 aagacaactt tctgcaagag acaaactatt ttatgctcgt tttttttctt acggttgcag
     7141 tggtgtatta gtcaattgga tcacgaatga atatgaagaa acaccgatgg aaatcgctat
     7201 gcagcttttc cgtttagcca aggatacgga attatattct tatcgtcttt atgcaaaaga
     7261 agagttggaa gaacagtaat agaaaaattt tttgttggaa aagcaataat cgctaaaagg
     7321 agatggggaa atgacaaaaa ttttatttgt ttgtttggga aatatttgcc gttcaccgat
     7381 ggcagaaggc ttgctgagaa aacgaatagc aatagaagga agggaagcag aattttttgt
     7441 ggattctgct gctacaagca cgtatgaagt tggaaagaca ccgcaccctg gaacgaaaaa
     7501 aatattaaac caagaaaaag tagatatgac gaatatggtt gctcggcaaa tcacacccca
     7561 cgattttgaa acttttgatt ggatcattgg catggatcaa gaaaatgtag aagaactgaa
     7621 acgaagagca ccacgctcag cgcaggggaa aatccatttg tttttaagta gcattcctgg
     7681 aaaagaaaaa gaaaatgttc ccgacccata ctataccaat aactttgatg agacatatca
     7741 cttgatcaca gaaggcctga atcattggtg gaatatttgg attgacgaaa ataaaataac
     7801 aaacaatttg taaagttgac gctttctttt taaggcgatg ttagagcatt tccttgtttt
     7861 tttgtaaaat gttaggtaaa ataaaattaa ttaagtaaca ctttcttagc taaaggagga
     7921 gtgcattcga tgaacgaaga gacagtatcg ccaatgaaga agcttacgaa gttagttact
     7981 catacttcgt atcaactttt tgattctttt gtaaatgaca ctgaagaaca tgctaagaat
     8041 gtacgattgc aaggaaagtt tattcttgag ctgcaaaaag ctgctactga acatagtcta
     8101 gttatcttac aagtaagagc gaatcgtttg agtagcaagt atgaaacgat ctcaggctgg
     8161 gtggcgacaa aaacggtcga aaaagaccga gtgatgatcc gcctacaaaa cgaagagcag
     8221 cagatccgga tggttcctgt agaacaaatt gaaaaaataa caatcctttc accagcaggg
     8281 gaacaggaac gattatcaaa ataatttata ccagatagaa aagcacgtat gagcttgtac
     8341 catcaatcat tactaatcaa aaataaccga atgctttaag ctgatatcag taaaacggaa
     8401 cgttttagga tgagtatcac agcatgacag cattgggttg attagttgaa tcgattggat
     8461 ggtacaagtt tttttgataa aaaaaggtcc tgctagccgg ggagctagca ggataaggag
     8521 ttaaaaatga aaaagtgttg ttagggttgt ttgttggtat gcttatataa tagtcactag
     8581 ttgtgaatat tttgtgacga aagttttcga aagttgttaa gtttttttgc gaaaactatt
     8641 tttatatcat atatatgtct aaaatctata ttataaaaga aatgttgttt gatcagcttc
     8701 tcttcggtat tagttcaaaa caaaacggca aagtatatgg agctactttg ccgttttgtt
     8761 tctctaatac tcaggtcagt ttatagacgc tcaataaacg gtgttcaaaa gccagtttct
     8821 cggtattaag tcaaatttat gcaaaaatgt gagaagccat tttcacaaaa tatttcttaa
     8881 tacttgaaac taagcggctt tttcacgacc tctcaataaa cggtgttcaa aagtcagttt
     8941 ctcggtatta agtcaaattt atgcaaaaat gtgagaagcc attttcaaaa aatatttctt
     9001 aatactcgaa actgagcggc tttttcacga cctctcaata aacagtgttc aagagacagc
     9061 ctatcggcaa taaggctgaa cgtctctttc acaacctctt ttatcaatct gctacttctt
     9121 ctttcttagc gaaactccaa atcagtccga taacaaaacc gataactgct ggtacgatcc
     9181 accccatccc caatttgaaa aacggcaagt agctttccgc aacatataaa agattttgaa
     9241 caataggtgt ttgtttgata gtttctgggc aagcgttcaa cccatcaaaa atcgaagcaa
     9301 tcaatgtaaa ataagtagtc attcgataaa cagccgtacg ctgcttaaac aaaggaccaa
     9361 ctaacactag taaaatcagt gtcattgcta atggatagat gaacatcaaa acaggcaacg
     9421 aaaattgaat gatattcgtc aagcctacgt ttgccgctaa acaaggaagg atactagcaa
     9481 gtgcaacaaa gaatgcgtaa tttctttgcg gaaacaaatc tgtgaatgtt tctgcaaaag
     9541 ctgtaatcaa accaataccg gtcttcaagc aagctaatat aacgataaat gctaataaga
     9601 tactgccata agtacctaaa taatgatcag caatctgagc cagcgtgatc ccgccatttt
     9661 cacttagagc aaagctacct aagctcatag cacctaaata agcgagcaaa gtataaatga
     9721 ttcccatcaa tataatgctg atcgcaccag atttgatcgt gtctttagcg atttcagcag
     9781 gttttgttac acccatgttt tggattgttg tgacaatgat gatcccaaat gctaaagcag
     9841 caagcgcatc taatgtgtta tatccttgag taaaccctgt gaaaaaagca tgctcttgat
     9901 aatttggctg aataggcgct tgatttacag aacctagtgg attcaaaaaa gcaaggagaa
     9961 gcaaagcacc taataaaacc aaaaatgcag gattcaggaa ctttccaaca taatctaata
    10021 tctttgtcgg ttttcttgaa aaccaccaag ccagcacaaa aaatacaata ctgaaaattg
    10081 ctagatatag cgtctgtctg ctaggatcga taaatggtgc taaaccaatc tcgaaagatg
    10141 tggttgcaag gcgaggtaaa gcaaaaaacg gtccgataac taagtaaagc aagatggtaa
    10201 aaaccatcgc atatgtttga tttatccgtt ttgctaattc atatacacct gagcttttcg
    10261 atacaccaat ggcaatcacc cctaagaagg gaagaccgat accggtaacc aagaaaccta
    10321 gattcgccca aaaggtagca gcgcctgctt cttgacccaa atgaacggga aagataagat
    10381 ttccagcacc aaaaaataga ccgaaaagca tggagcctat aaaaagatag tctttaaatg
    10441 ataatttttt tgtcatacat aactccttta cggttttttt aaaagtaaca aaatttcgaa
    10501 aaatatgcaa caattttcag aaaatttcct atataaaacg acggttattt aataacgtaa
    10561 gtgataaaat aaaagcgctg aatcatctga ggaggaatca tcgtgacaaa gtcgagatta
    10621 gaagcattta ctgatggagt ggtagccatt gttttgagag ttcttgtatt agagatcaag
    10681 atccctaata catctagctt tcattcttta tgggtcatcc gaaatacatt attagcttat
    10741 accatcagct ttatatttgt tgcggtgatc tgggtgaacc atcatcgtat atttcagatg
    10801 gcagaacgaa ttaactaccg agtggtctgg tcgaatatct ttttgctctt ttggctgact
    10861 ttatgtcctt ctgttactag ttgggtcggg cgtaaccctg aagctttttg gcctgagatg
    10921 gcttatgtct tagtatacac gatgtggagt ttttcttttg gtatcttgat gaggcaaatc
    10981 atgaaagcaa ataaaccaga ttcccatgtc gtgagagttt tgtcaaagga ccgtcgaagt
    11041 cttatctcca tgatgattaa cttatgcttg attgccggtg tcttttttgt accgttgatc
    11101 gggttattcg gacgtttttt tgtatctggt atttggattt tttcctataa aaaagcagat
    11161 gcctattatc agcggttgtt tccggggaaa tagaaaagag gctgggacag aagcttttaa
    11221 ctccaagaaa taagccgaaa tttcacaaaa atttaagaag caattttcgt aaattccttc
    11281 ttatttctcg aagctgacca cttctgtcac aactactttc ttacttctta cgttcatatt
    11341 tcatatgata gattagtaat agaagcaaga accacacagg tgttagtaat aaagcgattc
    11401 tcgtatcttt tcctaggaac aagacgacac acacaaaggc catgtaaaga aataccagat
    11461 aattactaaa tgggtacaac ggcattttaa atgaatgagc ttttttgccg aggatacttt
    11521 tacgatattt catgtgcgtg tagacaagca tactccagat aaacaagaaa caagtagtgg
    11581 caatactgct tactagtgtg aatacttcac taggcatcac gtaattcaag ataacagaaa
    11641 tgaaaatgac gatagtagaa aatgtcaaag cattccctgg cacatgatgt gttgttaact
    11701 ttttgaattt tttaggggca ctgccttctt gcgctaaaga acgaagcata cgtcctgtac
    11761 tatatattgc actgttgcag gcagaagctg ctgcacttaa tacgactaaa ttgatcaagg
    11821 aagcagcaat cgtgatccca atttccgaaa atacttctac aaatggactg ttatctggat
    11881 tcaagctgtt ccatggatag attgccataa tgacaaaaag agatcctaag taaaataaga
    11941 tgatcctgat aggaatatta ttgatagctt ccggcaacac tttttcagga ttttctgttt
    12001 ctccagcgac taatccaact aattctatcc cagcaaaagc aaaggttacc atttggaaag
    12061 ctaagataaa tccttttgca ccagttggaa agaatccgcc atgagaccat agattcgtga
    12121 tactggctgt gccaatatct gtcgtatagt gggtgaggat catataagct cctacgagga
    12181 taaaggcaat gatcgctaca actttgatca atgcaaacca aaactctaac tcaccaaaca
    12241 atgagacagc caccatattc aagcaaagaa gaagacctaa tgcaatcagt tcaggaagcc
    12301 actgaggaac accaggcgcc cagtagcgta catacattcc gatggctgtt aaatcggcca
    12361 ttgcaatcga tacccagcag aaccaatacg tccagccagt gatgaaagcc gcttttttgc
    12421 caagatattc tgcaacaaaa tccagaaaag aatggttatt ggtgtttgat aacagaagtt
    12481 cgcctaacgc acgcataatg aaaaaacaga tgcatccagt aataagatag gcgagcaata
    12541 tcgaagggcc agctagctgg atagactttc cggaacctaa aaacaacccc gttccaatcg
    12601 ctccgccaat agaaatcagt tggacatgtc tatttttcag tccacgctgc agttcttgct
    12661 cttcattcat ttttaaatca tcctaactaa aaaagtttaa ttacttccat attaaaagag
    12721 ttttctgaaa aacacaacta agattccaaa attttcagaa atttaccttt tatgagtaat
    12781 tcataaaaaa gaactgcggt aaacaccgca gctctttgtc ccaatcagct attgatccta
    12841 agcatttaaa atacgtgaaa ggaattcttt tgtacgttct tctttcggat ggacaaagat
    12901 atcttctggt gtaccttctt ctgcaatcac acctttatcc ataaaaatga cacggtcaga
    12961 tacatctttt gcaaattcca tctcatgcgt cacgataagc attgtcaagc cagtatgtgc
    13021 taagtctttg atcgtattaa gcacttcgcc aaccatttca gggtctaacg ccgaagtagg
    13081 ttcgtcgaat agcaatacat ctggattcat tgataatgcc cgagcaattg cgacacgctg
    13141 tttctgtcca ccagataatt gtgctggttt ggcttcgata taacgatcca taccaacttt
    13201 ttccagattt tcgattgcta cttttttggc agtttcttta tctcgtttca ggacagttgt
    13261 ttgaccagac atacagtttt ccaagacatt catattgttg aacaggttga aggattggaa
    13321 aaccatacct agattcgttc gatatttagg taaattatat ttaggagcta atacattttc
    13381 tccattataa atgatctcgc cgcctgttgg tttttccagt aaattgatac agcgcaagaa
    13441 agtagattta ccagaaccgg aagaaccgat gatcgtcaca acttcacctt tattgactgt
    13501 tacgctgata tctttcaata cttcattttc accgaagctt tttcttagat gtttgatttc
    13561 aataattttt tcattcatga ttcttcctcc ttattcctgc aaatttgaat tgtcgatttc
    13621 ttcaagcttg gtataagctg aaggaccatc gattttcttt tctaccgccc gcaggattct
    13681 ggtgatcgta aaggtcatga ctaagtagat gatcccgacg atcgtaaatg tttggaagaa
    13741 ttggaaattc gcaccggaag cagagtttcc ttggaagaac aaatcagcta cgccgattac
    13801 acttaaaact gctgtatctt tgatattgat aacaaactca tttcctgttg ctggcaagat
    13861 attccgtaaa acttgtggaa taacaacttt tctcatcgtt tggccatggg tcataccaat
    13921 ggcttgcgca gcttcgaact gcccttcatc tactgcaaaa atccccccac gaacgatctc
    13981 tgtcatgtaa gctccagtat tgagagagac aataaacaaa gcggcaatcg ttcggttcaa
    14041 tgaaatgcca aaagcgagtg ccagaccgta gaagatgacc attgcttgta ccatcatcgg
    14101 tgttccgcgg aatacttcga catagataga aagaagtgca tttccgattt tttggaagaa
    14161 gcgagtcagt gcatttcctg attcaggtac cgaacggaag acaccaatca gtaatccaag
    14221 tgtagtaccg acaactgtcc cgatcaatgc gatgaacaag gtaagtccag cacctcgtaa
    14281 gaacatattg ccgtattgtt tccaaatcgt agtgaaatca gaaatgatgc caggtttttc
    14341 ttcactatca gttgcagcag gctgatcttt gactgcttgg tccataattt tggttcgttc
    14401 atcgttagag atgccagaaa gaatctggtt tactttttgg atatccgggt cgcctttacg
    14461 cataccaaca gaaatctgaa catcttccgg gtttgtctgg aagccatctg caccgctgaa
    14521 ttcaagcatt tccaagtctt tgttcacact agttgccgtt acaccttccg gacgttcgct
    14581 gacataacca tcgatcattc cagaagctaa agctgttctc attgctgaga aattatccat
    14641 tgcttgttgt ttctgcacat ctggtatctg atcgatcaca gtatagtgga atgtatttaa
    14701 ctgagcagta atctttgctc cagaaagatc tgctaaactt tttgcttttg catatttgct
    14761 atctttttga actactacta ctaattgtga ttcgtaataa ggatcggtaa aatcaatttc
    14821 ttttcgtcgt tcagcagtag ggctcattcc ggcgatgatc gcgtcgatct tgcctgattg
    14881 aagggcagga gccagaccat cccatttcgt ttggacgatg actaattttt tccctaaacc
    14941 atctgctact tttttagcga tttgaacatc ataaccgccg gcgtaactat tttggccttg
    15001 aattggcaag gcattattct catctgtttg ttgagaccag ttgaagggtg catacccagc
    15061 ctccatacct acgcgaaact catcgctcgg tgttttttca tctgcatttg ccagagatcc
    15121 cacactgaat agtgagaaga caaaagcaaa tagtacggca actgaaacca tatttttttt
    15181 catttttttc tccctttctg attatatgat acacgagcgg tctagatgaa atgcggtaaa
    15241 gctttatctg aaaaaataaa aagcgaccga tttatagata taaaaaggcc gctaaaattc
    15301 cataaggtga taaacctctc acaaaacata gcgcaactta gcatcactgc taagacagtc
    15361 cgcaagctct taacttgcgt cccaacatac actccaagca aaaaagtgca catttcggcg
    15421 atcctcccta ttcctgcttc tccatgtttg cttcactcca cagggttaat gattctcgcg
    15481 acctctacct cattgattga ggcatctcgt attcagttat ttatacaaaa aaagtttacg
    15541 atactcgaga ggctttgtca atctataact gctgtttttt gaaaaaatac tgataaatat
    15601 agaaatcttt acttttaccg aaaaaacaat caaaatgtga atcatctatt cattgcaaac
    15661 gatagtataa ttatacacaa aaatgtatgt aatacaataa aaaaagaact agaacagaaa
    15721 atttcctgtg tccagttcca tctcgatcat ttaagctgcc gctttctttg gctcacgtcc
    15781 taggatcgct tgcaaaagaa caacgacgcc ataaatggct aatattagat aaatcaaact
    15841 tgagtattgg gtgattcccc agccccatgt tcgatatacg gctaatgtaa tggctaaaac
    15901 aattgcggca atcacgttga ataaagcaaa agcccaaaga tttccatttt ctttgcgtac
    15961 aagataaaaa acagagaaat aaacgcttaa cgctaaccag gcgatacaag ccagaataac
    16021 gccccaataa ataaagaatg gtaacatatc gattcacctc acttttatac agcagtattg
    16081 taacacgttt tcattctttt gtcagtgaac attgtgataa attgttcaaa tgcccataac
    16141 acttttttat tcccattttt ctgacttttc aacaataaat ttgttcgagt atgggctata
    16201 atggaaagtg aggtgacaaa acatgaaaca attaacagta gacgaagcag caaaagagat
    16261 attagcttat cagcaaatta catttttgac cggtgcaggg gtatccacac cttctggagt
    16321 gccagactat cgatctttgt caggaatcta ccacgggatg gagcagccgg aatacttgct
    16381 gagtcatcaa gcaatggtga atgaaccgga taaattctat caatttgtca aaaagattta
    16441 tcatcctgaa gcaaaaccca atatcattca tttggaaatg gcacatctag cgaaagaaaa
    16501 aaatgtttgg gttgtttccc aaaacatcga cggtttgcac gcaaaggcta aaagcccgca
    16561 actcgtcaat tttcatggaa gtttgtatca atgtcattgt cgaaaatgtc aacaaacggt
    16621 agactggaaa gaatatcttc acagcgacaa acatcaagca tgcggaggac aaatacgccc
    16681 agacatcgtg ttatatggtg aagggtttca agatggagtc atggagcagg cggcttatgc
    16741 cgtcagccaa gcagagctga tcgtcattgt tggaacgagt tttcaagttc atccgttttg
    16801 tgatctggta cagtttcgcc agccgtcagc caaaatctta gtaatcaatc aaacgcctgt
    16861 ttatttagcg gagccttata cgtttattca agaagacgga gcggttgttt tcaaaaaaat
    16921 acaagaatta ggagcagatt atgactgaat tcaaaaccga aaaagaaaaa atgattgcag
    16981 gcgagcttta ttttgccaat gatcctgagc tggttgccga ccgtatgttt gcaagaagtc
    17041 aaagccaaat catcaaccaa gcagaatcag cggaactgcg atcccaattg ttgaaagaga
    17101 cttttggccg tacagggaaa aaaatctata tggagccagt tatcaatttt gattacggat
    17161 ataatatatt tgttggggag aatttttatg caaatttcaa ttgtacattt ttagatgtca
    17221 gcacgattga aatcggtgat aactgcatgt tcgctccgaa tgtccagctt tacacggcga
    17281 cgcatccact acatccagtg aaacgtaaca gcggtctgga gtacgcaaaa cccattaaaa
    17341 tcggtaataa tgtttggtta ggcggaggag ttatcgtcac gccgggtgtc actctagggg
    17401 acaacgtagt agtcggcgca ggttcggttg tgaccaaatc ttttcccgac aatgtagtga
    17461 ttgcaggaaa tcctgctcgt atcatcaagg cagtagagga agaactaaca gaagagtctc
    17521 ttgaaacatt acgtcataag atcgatatga tcgatcgaga gttggttgct ttattagaaa
    17581 aaagaatgga tacggtaact aaaataggac aagtgaaaaa agaagaaaac caagcagttt
    17641 atgatgcaaa aagagagcag caagtattgg ataaagttgt tagtctgctg aaaaataagg
    17701 gatataagga aacaataaca gatacgtatg ttgatttgat gaaacactcg cgcgaatacc
    17761 aaaacaaaat gaaggaggaa taagtgtgat cgaaggagag acacggcgaa aacaaatcat
    17821 gcaaaccttg gcaaatacag aaaaaccagt gagtgctagc aagtttgcga aaacttttgg
    17881 agtcagccgt cagatcattg taggcgatat tgctttacta cgagctgcag gtaaaagtat
    17941 cgtggcaaca gcaagaggtt atatcctcga aacagaagaa accaatactg ggcacatcag
    18001 taaaatcgct gtccagcatg gaaaagatca aacagaagaa gaactgagac taatcgttga
    18061 aaatggtggt gaaatcattg atgtaattgt ggaacatccc ctttacggtg agttagcagg
    18121 aactttgcac atcaaaacgc cacaagatat cgattccttt atgagacgtt atcaaaaaag
    18181 taatgctact ttgttatccg aattaacagg cggcatccat ctgcatacga tccgctaccc
    18241 agaaaagcat atgctgaaaa acatcaaaca aaaactagct gaagcaggaa tcctctacga
    18301 aggataacat cgtagctgct actttttatc ctagaaacta ttctatcatc tctgagataa
    18361 acacccatat ggaagaaata agaaaaaata agaaaagatg cgtatccaat acacagaatt
    18421 ttttggaatt gcgatttcta gatgtctatc taaatttcga ttagtggaga aacataaaaa
    18481 agggaagtgc agcttttgag cactcccctt ttttataaag ctttcttgta gcatagtatc
    18541 gaaggaacct gccaattctc tactcgatca ccaatacgcc ttcttttagt gcaaatggat
    18601 tttgatcgtt gatgtggtca tagaacatca caccgttgat atggtcgatt tcatgttgaa
    18661 cgacgatcga ttcataattc ttcaaacgaa tcttgtgttt ttcaccattc atatcataat
    18721 aagacacagt gattttggca tggcgaacaa catatcctgg tacttctcga tcaacagata
    18781 aacagccttc accttcccct aaacaagcat cttgaacaga atggctcaaa attttaggat
    18841 tatacataac agtacttaga gaaggtccat ctgcctctgg gtcaggactt ggtacgtgaa
    18901 cagcaatgat tcttttagaa atatctaatt gaggagcagc taagccaaca ccgcctcgta
    18961 agtgtagttc ttcagctttg attggatctt ggctgttttt taagaactca agcatctctt
    19021 ttcctaaaga gatatcttct tcgcttaatg gaagagaaac ttcttttgca acttcgcgta
    19081 aggtcggatt tccttcacga atgatatcat ccattgtaat catggaatat gtaccccttt
    19141 cttaaaagac tgtgtttcaa gtctattttc gcattatagt ttaccacaaa aatggaaaag
    19201 tagtagtaag attgtttgaa aactaccttt tcttaatgat taactacttg attttcaacc
    19261 cggtttcagg taaagtatag ctgtgtgctc taggcacatg acctgccact tggttaagcg
    19321 aatcaccaac gttttactca gtcgcagggg aataatcgaa agaggtgaaa gaaatggcaa
    19381 tttcaaaaga acgcaagaat gaaatcatca acgaatacgc tcgccatgaa ggggatactg
    19441 gttctccaga agtacaaatc gctgtattaa ctgaagaaat caaccacttg aatgaacatg
    19501 cacgcgtgca caaaaaagac catcattcat accgcggcct aatgaaaaaa gttggtcacc
    19561 gccgtaactt gttagcttac ttacgtaaaa ctgacgttca acgttaccgc gaattgatca
    19621 aacgtttagg cttgcgtcgt taattttagt caaaaagagt gggattcaca tgaatctcac
    19681 tttttttact cgtatatcaa ttaagtaaaa ttccgtgatc aagctagatg gactcctact
    19741 aaccaaatga aagctgctca aaagacggct ttcggcagtt ttcatttgtt tagtagcgtc
    19801 ttgctttcac gagaaggaaa aggagaagtt atgtcagaaa aacaagtatt caaaacgaca
    19861 tggggcggcc gtcccttaca aatcgaagtc ggtcagctag caaaacaagc aaacggagcc
    19921 gttttagtcc gttatggaga tacagtagtc ttaagtgcag ctgtggcctc taaagaagca
    19981 aaagatacag actttttccc attaacgatc aattatgaag aaaaaatgta tgcagcaggg
    20041 aaaatccctg gaggtttcat caaacgagaa ggccgtccaa gtacggaagc aacattgact
    20101 gcccgtttga tcgaccgtcc aattcgcccg atgtttgcag aaggcttccg taatgaagtc
    20161 caagtaacga acatcgtgat gagtgtagaa acagactgct cgccagctat ggcagcaatg
    20221 ctaggttctt ctttagcact atcaatctca gatatcccat ttgacggccc aatcgcaggc
    20281 gtagaagttg gtcgtgtaaa tggcgagtac gtcttgaatc caacagtaga acaagcagaa
    20341 caaacagata tcgaattgac tgttgccgga acgaaacaag ccatcaatat ggttgaaagc
    20401 ggcgcgaaag aagtttcaga agaagacatg cttggtgcat tgctgtttgg ttttgacgct
    20461 atcaaagagt tagttgcttt ccaagaagaa atcgttcaag cagtagggaa agaaaaaatg
    20521 gaagtgactt tactgcaagt tgatgaagta ttgaaaaaag aaatctttga tgcttcttac
    20581 gctacaatga aagcagcagt aatgactgaa gaaaaacttg ctcgcgaaga taatatcgag
    20641 caggtgaaga tcgatatccg cgaagcttat gctgagaaat ttgcaggtca tgaagatgaa
    20701 gatcacttgt tgaaagaagt taaacaaatc acagaagatc ttgaaaaaga tgtcgtacgt
    20761 gaattgatca ccatcgataa gatccgtcct gacgggcgta aattggatga aatccgtcca
    20821 ttgtcatcag aggtcagctt attgccacgt gtacatggat caggtttgtt cacacgagga
    20881 caaacacaag ctttatcagc ttgtacactc gcacctctag gagagcacca aatcattgat
    20941 ggtttagggg tggaagtcag caaacgattc atccaccact acaatttccc tcaattttct
    21001 gttggttcta caggacgtgc aggctctcca gggcgtcggg aaatcggaca tggcgcatta
    21061 ggtgaacgtg cgttagcaca agtaatccct tcagaagaag aatttcctta tacgatccgt
    21121 ttagtagcag aagtattgga atcaaatggt tcttcttccc aagcaagtat ctgtgctggc
    21181 acattagcat tgatggatgc aggtgtacca atcaaagcgc cagtcgccgg tattgcgatg
    21241 ggacttgttt ctgatggtga aaactatacg atcctaactg atatccaagg gctggaagat
    21301 catcttggcg atatggactt taaagttgcc ggtacaaaag atggtatcac tgctttacag
    21361 atggatatca agattcaagg aatcacagaa caaatcctaa ctgaagcatt aactcaagca
    21421 aaacaagcac gtatggaaat cttagaagaa ttgacttcaa cgattgctgc tccacgtgaa
    21481 gaattaagcc aatatgcacc aaaaatcgaa atgatccaga tcgaacctgc taaaatcaaa
    21541 gatgttatcg gtaaaggcgg cgatacgatc aacggaatca tcgatgaaac tggtgtgaaa
    21601 atcgatatcg atcaagatgg aaaagtaagt attgcttcag ctgatgcaga aatgatcaaa
    21661 aaagctatca agatcatcga agatttgacc aaagaagtca aagttggtga agtttatcta
    21721 gggaaagttg tccgtatcga aaaattcggt gcattcgtca acttgatcaa aggaaaagat
    21781 ggacttgtcc atatttccca attagcaaac gatcgtgtca ataaagtcga agatgttgtt
    21841 aaactaggtg atgagatcct tgtgaaagta actgaaatcg ataaacaagg acgcgtaaat
    21901 ctgtcaagaa aagcaatgtt gaacgaagat ggttcaacga aaaatgaaga aaaataattt
    21961 aattcatagg aataatgtac ttgtgatcgt gaaggtgaca gggggacaat aattattcga
    22021 aaaaagggga ctgtgacaaa agccgtgtca cagtcctttt ttttcgaata aacggtgttc
    22081 aaaagcttgc atctgcggta ataagtccag ccaagttaaa aacattagga gctgttttta
    22141 cttggctgtt cttattactt gatgcagaac agctttttta caacctctcc aataaacgat
    22201 gttcaatcag ctgtccctcg atattagctc aaaaaacggc aaaatatgag gaactatttt
    22261 tccgtttgtt tctctaatac tcaggtcaaa ccgctgattt cacaacctct gattcacggt
    22321 gttccagaag cagcttctac ggaaataagc tgaacacttt tgttacaact tctttttttc
    22381 tggagtttct tggagcatat ttttggagtc cttctgaata aagcgtaaaa taaaagtgag
    22441 aaatatggat aaaggaggga aaggagatgc tgaatttttt atggtcgtta tttatcggcg
    22501 gtatattagg tttgatagct ggcgcaattt taggaaagga agtcccggga ggcgtatctg
    22561 gaaatatcat tatcggtttt ttaggtagtt ggctcggcga atttttacta ggaccactgg
    22621 gaccggaact cggcggtttt tatttgatac cagcattatt gggtgcaatt ttttgccttg
    22681 ctctttattc atttgtggca agacgtatca gagaacgtcc aaagaaagaa taattttatt
    22741 caaataagtt gacacttcag tgtgttactt ggataattag aagcaattta ggaaagcggg
    22801 tgtcatggat aagaatatca tctatcattc aagcttacct aaaggatatc catataacaa
    22861 gaaatgaggg aaaaacgtgg caacaacgca caataccaaa aagatatcaa tggctggcct
    22921 gcttgtagcg atgggagtcg tctacggaga catcgggaca agtccgcttt acgttatgaa
    22981 atcgattgtt gaagataatg gcggattgtc aggaatcaat cctgattttg ttatcggatc
    23041 agtctcattg attttttgga cattgactct actgacaaca atcaaatatg tcgtgattgc
    23101 attgaatgca gataaccatg gtgaaggtgg aattttctca ctctacacac ttgtccgaaa
    23161 aggtgggaaa tatttgatca ttcctgcaat gatcggagga gctgccttac tagcagatgg
    23221 ggtattgacc cctgctgtga cggtaacgac tgcgattgaa ggcttgcgag gaattccgca
    23281 gttttttgac cggttcggta ataaccagaa cgtgatcgtc attatcactt tagttattat
    23341 ttttgtttta ttcatggtcc aacgctttgg tacagatatt gtcggaaaag cattcggacc
    23401 aatcatgttc gcttggttta cttttttagg cgtaatggga ttaatgaact tcagtcaaga
    23461 ctggacagtt atccgtgcac tgaatcctta ttatgctatc cagctgcttt ttagtgcaga
    23521 taacaaatta ggcttgttca ttcttggaaa tattttcctg gctacgacag gtgcagaggc
    23581 gctttattcc gatcttggac atgtcggcaa acgaaatatt cgtctgagct ggccttacgt
    23641 caaaatttgt ttggttatca attattttgg gcaggctgcg tggttgttga atgtttatca
    23701 aaaccctgaa gcacagcaaa tcaagaacct gaatccattc ttccaaatga tgcctcaaag
    23761 ttggatcgtg atcggcgttg cttttgcaac agtcgctgca gtcattgctt cgcaagcatt
    23821 gattacaggt tcgtttacgc tagtttcaga agcaatcaag ttgaaactgc tgcctcggtt
    23881 aaagatcatg tatcctggga acagtatcgg gcaaatgtat atcccagcag tcaatctgat
    23941 tttatggatc gcctgttctc tagtggttgt cacattccga acgtctcatc atatggaagc
    24001 tgcttacggt ctatcgatca ctgtgacgat gttgatgaca acagcactgc tttactttta
    24061 tcttatccaa aatgggcatt ctcgttggct tgcatattta gttacctttt tcttcggtgc
    24121 gatagaagtc gttttcttta tctcaagtgt tgtcaaattc ttccgaggcg gttttgtagc
    24181 cgttctgatt ggtttgatca tcttagtcgt gatgtttatt tgggaacaag ggaacattat
    24241 tcgcgaatcg tcaaccgagg atgtgtcttt gagagattat atcccgcagc ttagagaatt
    24301 acgtgatgat acttctttac caatctatca gacaaatgtg gtcttcttgg ttcctgatat
    24361 ggctgaagat aaagttggcc gacagttcat ctattcgatt ttagataaac ggcctaaaag
    24421 agcacgagtc tattggtttg ttcatgtgga agtgacggat gagccatata cgaaagaata
    24481 tcaagtagat atgatggaca ctgattttat tgtccaagtg aatctgttct taggcttcag
    24541 ggtccaacag gaaatcaacg tatatgttcg ccaagtcatc catgatttga tgaaacaagg
    24601 ccgtttgcca aaacagccac aaagatactc tctgacacca gggcgagaag ttggcgattt
    24661 ccaatttgtc ctgattgatg aagtagtttc caatgtgaca actctaggaa aatggcaacg
    24721 tcaaatcatg caggcaaaac tggcaatcaa aaaaatcgct acctcgccag aaacatggtt
    24781 tgggctagag tacagcgaag taaaacatga aactgttccg cttatcattg gaaatactcg
    24841 gaaaacatgg ttgaaagagc ggttgtgaaa gtagcgcttt gacctgaact aatgtcgaat
    24901 gtcagctact tgaacaccgt ttatcagagg tcatgaaaaa gctgtactgc aacaaacagt
    24961 aagaacgagc agataaaaac agcttctcat gtttttcatc tgtttgggct taatgccgca
    25021 gatgcaagct tttgaatacc gtttttatca gaggtcatga aaaagctgtt tagcaacaag
    25081 taacgcaatg attttgaagt gataaggaga tgtggctgaa aaatgccgca tctccttttt
    25141 tattcctaaa tatgaatacg cattattttc aatctttttg tagaaaaagg gtacaataga
    25201 aaaagagaaa ggcgggagaa tatggttatc aatttcatta ttttattttt gatgattgca
    25261 attacttcat tttttgtagc aagtgaattt gctttagtga aaatcagacg atcaagattg
    25321 gaacaactag aaaaggaaaa tgtaaagaac gccaagctag ctcttcatgt tacacaccat
    25381 ttagataact atttatcagc tagtcaatta ggaattacgc taaccggttt gatcatcggg
    25441 tgggtcggtg aaggatctgt cgcggcttta ctagaaccag tgatcggtaa actacctttt
    25501 agttcggcta ttagcagtac aatctctgtg gcgctgggat ttattttagt tacgtatatc
    25561 gatgtggtta ttggcgaact gcttcctaag agctatagta tcgtcaatac ggagaaggtc
    25621 gtcttgttcg tagttaaacc acttcattat ttttataaag taatgtttcc ttttatttgg
    25681 gtattgaatc attcagctgc agggctaggg aagctattag gtgtgcgtct cgtatctgaa
    25741 ggagaagaga cattgtctca agaagagctg acattagtcg cattgaattc ttatgaaaaa
    25801 ggagagttga ccaaagaaga atatcattat ttggaaaacg tgtttgaatt tgatgatacc
    25861 ttggcaaaag atatacaagt agaccgtacg tccatgcaag tattcgaagc ttcagaaacc
    25921 gtcaaagaag cgataaaagc ttcgattgaa caaggacata ctcgctatcc ggttatctta
    25981 gaatcaaaag acaatgtttt aggttatgtc accttgccag atttgatcaa gcaatcttat
    26041 aaagatgacc agctgacagt agaacagttg attgaagagc cgatcgtaac aactgaaacg
    26101 atccctataa aaaaattatt gacgatcatg cgaaaaaagg gaaagcatat tgctatctta
    26161 aaagatgaat acggagggac aagcggctta gtgacgattg aagatatttt agaagaaatc
    26221 gtcggggaaa tccgagatga gacagatttg gacgaagcat tgatagcgga acagtcagat
    26281 ggctcttata ttatctccgg caaactaaca ttagatgatt tccagcgtta ttttcatgta
    26341 gagattccag agtttgaaga aacgaatttt actacacttg cgggctttgc ttctagccga
    26401 tataaagaaa taaaagcagg aacaatcatt gagattgcat cattccgttt cacggtatta
    26461 gaataccagc atgcacatat cgattatttc aaagtagagt ccacggaaag aaaaacagaa
    26521 taaaagaaaa aaggctgtga cattttttgt cacagccttt ttcctacgat tagtcaagta
    26581 aaaggtgcaa gaaaaatagc tcgtaaacga aagcgacaaa atcaaagcac accaaataaa
    26641 gccccgttcg taaaaaattt tcgatctggg caaagaagtg gtatactgta agtggtttag
    26701 ttaatggtcc actgagaccg cgtacgcata gtcgtactct gtatgtgccc taaccatagc
    26761 gtgcatacat ttcagaagct tgttcataca agcaactgtg gcaaccttat cttt
//
LOCUS       DACEMK010000032        26632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEMK010000032 DACEMK010000000
VERSION     DACEMK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.032"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW715_001145"
     CDS             5..403
                     /locus_tag="IW715_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1021762.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW715_001146"
     CDS             387..1082
                     /locus_tag="IW715_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1021763.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IW715_001147"
     CDS             complement(1666..2553)
                     /locus_tag="IW715_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1021764.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IW715_001148"
     CDS             2738..3577
                     /locus_tag="IW715_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1021765.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="IW715_001149"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="IW715_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1021766.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="IW715_001150"
     CDS             5045..5401
                     /locus_tag="IW715_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021767.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="IW715_001151"
     CDS             5404..6561
                     /locus_tag="IW715_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021768.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="IW715_001152"
     CDS             complement(6587..7027)
                     /locus_tag="IW715_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1021769.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="IW715_001153"
     CDS             7187..7693
                     /locus_tag="IW715_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1021770.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="IW715_001154"
     CDS             7861..9774
                     /locus_tag="IW715_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1021771.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="IW715_001155"
     CDS             9779..10780
                     /locus_tag="IW715_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1021772.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="IW715_001156"
     CDS             complement(10982..11188)
                     /locus_tag="IW715_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1021773.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="IW715_001157"
     CDS             complement(11834..12682)
                     /locus_tag="IW715_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1021774.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12846..13748
                     /gene="gnd"
                     /locus_tag="IW715_001158"
     CDS             12846..13748
                     /gene="gnd"
                     /locus_tag="IW715_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1021775.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13750..14751
                     /locus_tag="IW715_001159"
     CDS             13750..14751
                     /locus_tag="IW715_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1021776.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14748..15032
                     /locus_tag="IW715_001160"
     CDS             14748..15032
                     /locus_tag="IW715_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1021777.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15065..15862
                     /locus_tag="IW715_001161"
     CDS             15065..15862
                     /locus_tag="IW715_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1021778.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15876..16709
                     /locus_tag="IW715_001162"
     CDS             15876..16709
                     /locus_tag="IW715_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1021779.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16727..17584
                     /locus_tag="IW715_001163"
     CDS             16727..17584
                     /locus_tag="IW715_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1021780.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17606..18115
                     /locus_tag="IW715_001164"
     CDS             17606..18115
                     /locus_tag="IW715_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1021781.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18152..18553
                     /locus_tag="IW715_001165"
     CDS             18152..18553
                     /locus_tag="IW715_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021782.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18665..19018
                     /locus_tag="IW715_001166"
     CDS             18665..19018
                     /locus_tag="IW715_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021783.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19052..19495
                     /locus_tag="IW715_001167"
     CDS             19052..19495
                     /locus_tag="IW715_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1021784.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19512..20597
                     /gene="uxuA"
                     /locus_tag="IW715_001168"
     CDS             19512..20597
                     /gene="uxuA"
                     /locus_tag="IW715_001168"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1021785.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20590..21303
                     /locus_tag="IW715_001169"
     CDS             20590..21303
                     /locus_tag="IW715_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1021786.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            21632..22933
                     /locus_tag="IW715_001170"
     CDS             21632..22933
                     /locus_tag="IW715_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1021787.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            23556..23756
                     /locus_tag="IW715_001171"
     CDS             23556..23756
                     /locus_tag="IW715_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1021788.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            24324..24887
                     /locus_tag="IW715_001172"
     CDS             24324..24887
                     /locus_tag="IW715_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021789.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(24948..25292)
                     /locus_tag="IW715_001173"
     CDS             complement(24948..25292)
                     /locus_tag="IW715_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021790.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(25389..25634)
                     /locus_tag="IW715_001174"
     CDS             complement(25389..25634)
                     /locus_tag="IW715_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1021791.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(25704..25958)
                     /locus_tag="IW715_001175"
     CDS             complement(25704..25958)
                     /locus_tag="IW715_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021792.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(26122..26445)
                     /locus_tag="IW715_001176"
     CDS             complement(26122..26445)
                     /locus_tag="IW715_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021793.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT         9232 a         4165 c         5391 g         7844 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa ggacgatcga
    14161 tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt tggtgtgatt
    14221 ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt tcgctgtgat
    14281 gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa
    14341 gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc gaatttgaca
    14401 gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga aaatgtttat
    14461 ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa tggtctgaaa
    14521 agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg acgtcaagat
    14581 catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc ctacacaagg
    14641 gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac
    14701 cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg atgaagaaaa
    14761 tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa gaaggtggta
    14821 tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg gtcacccacg
    14881 gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac gaaacgattc
    14941 aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga cgaatagcta
    15001 caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa aactgaccga
    15061 tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc ctactttagc
    15121 tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg gacaagcaga
    15181 agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc cccaattagt
    15241 gattgattcc cctgttccat atttcattgc cgggttagca gatacactag caaaatggta
    15301 tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt tgaagctagc
    15361 acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta aagcaatcca
    15421 agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga ttatctttgc
    15481 tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg cagcacatgc
    15541 tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac atggagaaaa
    15601 agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa aaatagacga
    15661 gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa tgggattatt
    15721 tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga aagagaaagt
    15781 gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga tggaactgga
    15841 aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt gtagtacaaa
    15901 gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca
    15961 cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg atcatggggg
    16021 aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg ggggtttttt
    16081 ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca gtaccagtcg
    16141 cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca gtcggattgc
    16201 tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt aaatatacag
    16261 acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt ctttatccaa
    16321 cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat ttaggggcgc
    16381 cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac attcttgaag
    16441 tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa
    16501 aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg aaatctttag
    16561 atattacgat ggttacttat gccatttttg ggattattat cgctttgatc tttgtccagt
    16621 cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca tcttttgatg
    16681 acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg ctgaaataat
    16741 ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct
    16801 gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg cgccatcact
    16861 actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact ctttaaaagc
    16921 agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg gcggtgtgat
    16981 tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg aagaaaaaga
    17041 gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg gggcactagc
    17101 aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg caatcgcggt
    17161 accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct gctttgggtt
    17221 gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg gccgtaatgc
    17281 tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat cagttttagg
    17341 gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt
    17401 tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg tgccagggat
    17461 attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag gattgaaagt
    17521 cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa tcggtatttt
    17581 ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg
    17641 tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga attccatttt
    17701 tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca aaaatgcagg
    17761 aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat tctattggga
    17821 tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc aaacgattta
    17881 cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga ttgctcaaaa
    17941 aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat cagatgctga
    18001 aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc aagccactcc
    18061 atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt cataaacaaa
    18121 tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg
    18181 ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt
    18241 tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag tcattgctta
    18301 cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa aagcagagaa
    18361 agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt tcatgtttga
    18421 ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca ttttgttgtt
    18481 cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg tattcgatct
    18541 gctatccgta taaacaggta tcactgtact agcatttggg agctactttt tcttattggg
    18601 aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt catccatatt
    18661 aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg cgtttgttga
    18721 tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga aaaaagaggc
    18781 tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt cgggaaatta
    18841 tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa
    18901 ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca aaacagattt
    18961 acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt ttcgataagt
    19021 aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg ttacacatgg
    19081 taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat cgagccatat
    19141 tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga aggtcaaaga
    19201 aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc taattagcgc
    19261 aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa agaaagcagc
    19321 cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca atcatcagat
    19381 gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa aagataattt
    19441 aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt tttagaagga
    19501 aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat tcgatcccat
    19561 taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg ctgactaaaa
    19621 tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct gttgagagcc
    19681 aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc aaggcaggga
    19741 cagatgaacg agattactat attgataact atatacgaac aatcaaaaac ttgtctcgat
    19801 gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg aaaactgatt
    19861 tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa atcgtcgatc
    19921 agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga ttcaaattgc
    19981 cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa
    20041 tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg attccagtgt
    20101 gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg gaactttttg
    20161 gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat gcagtagatt
    20221 ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt
    20281 tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc agaaacgtgg
    20341 agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg
    20401 atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct atacgccctg
    20461 atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg
    20521 caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa aaaccactgg
    20581 gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct tttattagaa
    20641 actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga ttatgaaatg
    20701 cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag agaacaaatg
    20761 ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat caatcaatat
    20821 aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc agaagaaatt
    20881 ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa tacacgggta
    20941 aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca aatcatttca
    21001 ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa
    21061 atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg gatcgaagca
    21121 gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg gttgcccgat
    21181 cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa taaagcagac
    21241 tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc
    21301 taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac tgagggaaca
    21361 gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt gaacaattta
    21421 gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa gagaagaacg
    21481 agaatcgtcc atatgagggt ttccacggct ctataatttc taggactgat agaaaatcat
    21541 tctatcggtc ccttttgagc atgaaaaaac aaaacataca gaagctccgt tagaattgaa
    21601 tcaccacaac acaatcaaag gagcgttctg tatgcctgat cttcatcata tcaaactact
    21661 acttggaatc aaagataaaa acattttcat cacaaatgtg gaatcgaaga gcattaagaa
    21721 tatcaagagt cttgttgttt ctgcgacgtt aagtaaagaa attcgtcgct gtcctttgtg
    21781 taaacagatg aatcatgaag gcatgatcgt taagaacgga aagaaaaaat cgctcattca
    21841 acttaataaa tgtgcgaacc aactgactta tcttgcttta gcgaaacaac gctaccactg
    21901 tcgtggctgc cacacctatt ttacggcgaa tacctacatt gtcgatagaa actgctttat
    21961 cgctaaacaa gtgcgctata aaattttaga agagctaact gaaaaacaag caatgacgac
    22021 aattgctaag cattgcggcg tttcttggtc gaccgtttct cggacactcg cttctctgat
    22081 ccccatgaca aaagtaaaaa gaaattggtt accgcgttgt ttattaatgg atgaatttcg
    22141 ttcactgaaa aatcaagtcg gtccctactc cttttcttgt atggatggcg atactggaaa
    22201 attactggat attcttcctt cacggaagaa aaaggctctc gtttcttatt ttatgcaatt
    22261 tgaacgacgc gcacgattga aggtaaaggt catcgtgacg gacatgaatg tttcttatgc
    22321 ctctctgata aaggaatgtt ttccaaaggc aaaactcgtt gtggatcgtt ttcacattgt
    22381 caaacatctc attcgtaaat ttgaggatat tcgcgtacga atcatgaaaa gctttgatcg
    22441 aaacgatcca acacaagcca aatattatcg acaattaaag gcgttgtctc ggcttctgat
    22501 caaacgacag gacaagcttg tttatgacaa atggaccaaa tggcgcaatt ttggttgggc
    22561 gtatctcacc gaaagtgaag tggtggatcg tctgctttcg atctctgacg aattacgaat
    22621 cgcctatttt tactatcaag aaattcttca agcgtttcac gacaaagagg cggacacttt
    22681 ctttaaactt gtaagaacga tgcccaattc cgttccaaaa gaactgcatt ctataaaaaa
    22741 agcttttata aactatgaat ccggcattcg tttagccctc gaattgcctt attcaaatgc
    22801 caaaatagaa aatctgcata cccacatcaa agcattgaaa cgagtcgctt atggctttcg
    22861 aagctttcgg aaaatgaaga ccagaatttt cttactaaat aatctgatta cctatgaatc
    22921 aaaaaacatc taaagtagaa aaggaattct accttagatg ttcttatctc actctatcag
    22981 tcctatttga caaagagccg tttccactcc ttatgcagat ttttttaatt cttgcactgc
    23041 ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa aaacgacaaa
    23101 tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat agaatgtcat
    23161 agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga taacgaaatt
    23221 ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt taaagtcaaa
    23281 agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa
    23341 ctttttcttc gattcgaaga caatagaaga caatgaattt tatatttcat tgcattatcc
    23401 ttgtgttatt agaaaaagca tgatatatta aatgagtaat ctattttgaa acgtaatttg
    23461 agcgatatat ctacatagat aaaactcctt ttgcctatca aatatttatt gcaacgaaaa
    23521 aacgtacatt gatacgtatc aggaggaatt tatttatgaa taacggtaca gtaaaatggt
    23581 tcaacgcaga caaaggtttt ggctttatca ctggtgaaga tggaaatgat gtatttgcac
    23641 atttttcagc tatccaaggt gacggcttca aaactttaga agaaggccaa gcagtgtctt
    23701 acgatgttga agatggtcaa cgtggccctc aagcaacaaa cattgttaaa gcttaataaa
    23761 aattgacttt taaccgtctt ttcgaaagaa aagacggttt ttttgtacga aaaaattcat
    23821 tggtcgttga atgggctgaa tagtagtgta taaaacaatt ttgtggagcg tgaagctttt
    23881 atggtagagg aaaatacgaa taaagtgatg ttgataaaaa gattcctatc ttgaatatga
    23941 atcgagatgt cttgaaaatg aatgagtttg accaatcatt atcctatcga aatttacagt
    24001 ttcgttcgat tggtctgtca attaatgaaa aagtaaaaaa gaatatgttc taggaagagg
    24061 gcggcacatc taaattttta agtaacggac agttcaaata tcaagcattg aaagaggcta
    24121 tcctactgtt tgaaggaata cgaatatata aaaacgacaa agaggaaaaa gtagttcatt
    24181 aaatcccttc aagtgatgaa agaatgagat ctaataaaac ctgatctttc atctgttttt
    24241 ttaataacgt aataattttt ttattttttg tgctttcgcc gttattgtag agtgatttag
    24301 cattcttatt tttaaaaata actgtgttat atattaaaaa aggcataata gttataagct
    24361 tttttaaaga atatttagaa attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta
    24421 ttattttagt ctattttcag ggggggaacc ttatgataaa aaaactattt ttactatcaa
    24481 tattaacgct agcaatggca ggttgttctg actctggctt agcaactgaa ctgatcacaa
    24541 aatcaactga agccacgaca gaggagttaa aatgtggaaa cgatcacagt gactacagag
    24601 gcttaaatcg acgatgcatg ggaatattag gcagaagcaa ttgtaagcat cgatgtagta
    24661 atcgaagaag gtgtagaaga agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc
    24721 tgtgctgtgc atcgtataca gacgtcgaag atgcggtact tttaaaccac gtcaggagat
    24781 gttcatttat gtctagggaa gttggcgatg aaccaaaact agagtacgct tttagttttg
    24841 cacgtgagga tgaagataac cctaaaactt acgacatttt agcttgatct aagatggtta
    24901 aagcttttta atgaagtaag aggagataga aggcttttct agttatatta ggtaagataa
    24961 attccttttt gctccaaatt tgctaaacat tctgctagat aaacttcatc gtgttccgga
    25021 taaatcggca cgttgcgctc tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca
    25081 cgatacatag gatttttcca ctctggtgct gggctgtaac cacgtttttc catttcatcc
    25141 atgacaagct gatgatattg gaataatcta taaggagaat agtgaaaaac ataatccaca
    25201 gtagcatgtt tctttcccca gccatttcct cttaatgcac agcattcccg atgttgtcct
    25261 aaaagctgtt gacgaggaag ctttgaaatc aatttttcat gccataatca catagaaatg
    25321 tctcctttat atatagattg ttaaccttag acgttaaatg aaaaaaccag caccaaggct
    25381 ggaaaaaatc acttcagttt gcttgctgtt tctgagcgac caattaggta atcaacggaa
    25441 atatcgtaaa agtcagcgat atagattaat tcaatgatgc ttggaatttc ttctccttct
    25501 tcgtacttac gatatttgaa taaaggaata cttaacgcgc tagaaacagc tacttgagat
    25561 aaagatcggc cagttcttaa gaaattcaga cgacaacgta aagtatccag taaaatagct
    25621 tcttcgatac tcatttaatc acctttcatt atagtagaat agttataatg actatagaaa
    25681 gctacacatg caatcaatag tactcaatgt tttttattta aacgtatcaa ataaagaatg
    25741 acgatcaata aagacaaaac cagtcctgat aattcttcta taaaaacact atcgggttca
    25801 aagtagttgt aattaaaaac tttccacata aagtagatag ccggaagagt caaaatagct
    25861 aaataaaacc gttctttgtt ccaattcata agataaacaa tacttgcgac cagtgcgacc
    25921 gcaggcaccc aaaacaaaac tctcaccaac gtaatcatgt aacggactcc tcaacgattc
    25981 atataataaa agataacatg ttattgcata catcacaaat gaaatgctag gaaaataaaa
    26041 gaagaataga tcaatgaaaa atgaaaaagc agaacagttt tctgctgata agcaggaact
    26101 gttccgctga atgtgtctta tctatcgaga aaaaatcaat ggattgatat ctaagtaatt
    26161 caatacatat aaaaagagga cgatcgcatt cactaaaaag gaaagaatga tgatcccata
    26221 gcgaatggaa acatatttaa caaatacttt tgtttgaata cgggcatatc tttttccttt
    26281 atagctgagc atcatgacaa gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat
    26341 aaacagaatt cctgaggttt gataatttaa ttggagaaaa taagctgctg ccgcaactaa
    26401 atcgaataaa atcgcaataa atggcaaaat aatcactcct aacattgaga ataaataaaa
    26461 gaataacatt ttttctaaaa taagcaaata aaaattaaag taacaaaata gttatgacac
    26521 aagtaaaaga aataagaata acaatattat tttacttata ttttgaaact tgtgagagtt
    26581 tgtcattcgt gctatagttg aaaagtaaca aaaataaatt ttatttatga tg
//
LOCUS       DACEMK010000033        26545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEMK010000033 DACEMK010000000
VERSION     DACEMK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.033"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..343
                     /locus_tag="IW715_001177"
     CDS             <1..343
                     /locus_tag="IW715_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021794.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            424..1044
                     /locus_tag="IW715_001178"
     CDS             424..1044
                     /locus_tag="IW715_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021795.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1374..2600)
                     /locus_tag="IW715_001179"
     CDS             complement(1374..2600)
                     /locus_tag="IW715_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1021796.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2672..2830)
                     /locus_tag="IW715_001180"
     CDS             complement(2672..2830)
                     /locus_tag="IW715_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021797.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3518..4258
                     /locus_tag="IW715_001181"
     CDS             3518..4258
                     /locus_tag="IW715_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021798.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(4350..5537)
                     /locus_tag="IW715_001182"
     CDS             complement(4350..5537)
                     /locus_tag="IW715_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1021799.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            5859..6701
                     /locus_tag="IW715_001183"
     CDS             5859..6701
                     /locus_tag="IW715_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1021800.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            6698..7648
                     /locus_tag="IW715_001184"
     CDS             6698..7648
                     /locus_tag="IW715_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021801.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            7663..7896
                     /locus_tag="IW715_001185"
     CDS             7663..7896
                     /locus_tag="IW715_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1021802.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            8227..8811
                     /locus_tag="IW715_001186"
     CDS             8227..8811
                     /locus_tag="IW715_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1021803.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            8942..9610
                     /locus_tag="IW715_001187"
     CDS             8942..9610
                     /locus_tag="IW715_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1021804.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            9612..11036
                     /locus_tag="IW715_001188"
     CDS             9612..11036
                     /locus_tag="IW715_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1021805.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            11054..11623
                     /locus_tag="IW715_001189"
     CDS             11054..11623
                     /locus_tag="IW715_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1021806.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            11733..13487
                     /locus_tag="IW715_001190"
     CDS             11733..13487
                     /locus_tag="IW715_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021807.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            13441..15213
                     /locus_tag="IW715_001191"
     CDS             13441..15213
                     /locus_tag="IW715_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021808.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            15736..16005
                     /gene="rpsN"
                     /locus_tag="IW715_001192"
     CDS             15736..16005
                     /gene="rpsN"
                     /locus_tag="IW715_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1021809.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            16020..16199
                     /gene="rpmF"
                     /locus_tag="IW715_001193"
     CDS             16020..16199
                     /gene="rpmF"
                     /locus_tag="IW715_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1021810.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            16231..16608
                     /locus_tag="IW715_001194"
     CDS             16231..16608
                     /locus_tag="IW715_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021811.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            16629..16778
                     /gene="rpmG"
                     /locus_tag="IW715_001195"
     CDS             16629..16778
                     /gene="rpmG"
                     /locus_tag="IW715_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1021812.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            16802..17785
                     /locus_tag="IW715_001196"
     CDS             16802..17785
                     /locus_tag="IW715_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1021813.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            17815..17937
                     /locus_tag="IW715_001197"
     CDS             17815..17937
                     /locus_tag="IW715_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1021814.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            17955..19478
                     /locus_tag="IW715_001198"
     CDS             17955..19478
                     /locus_tag="IW715_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1021815.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            19502..19741
                     /locus_tag="IW715_001199"
     CDS             19502..19741
                     /locus_tag="IW715_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021816.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(19934..20395)
                     /locus_tag="IW715_001200"
     CDS             complement(19934..20395)
                     /locus_tag="IW715_001200"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1021817.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(20590..20808)
                     /locus_tag="IW715_001201"
     CDS             complement(20590..20808)
                     /locus_tag="IW715_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021818.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            20926..22542
                     /locus_tag="IW715_001202"
     CDS             20926..22542
                     /locus_tag="IW715_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1021819.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(22783..24123)
                     /locus_tag="IW715_001203"
                     /pseudo
     CDS             complement(22783..24123)
                     /locus_tag="IW715_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(24399..24554)
                     /locus_tag="IW715_001204"
     CDS             complement(24399..24554)
                     /locus_tag="IW715_001204"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1021820.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(24746..24940)
                     /locus_tag="IW715_001205"
     CDS             complement(24746..24940)
                     /locus_tag="IW715_001205"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1021821.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(24850..25182)
                     /locus_tag="IW715_001206"
     CDS             complement(24850..25182)
                     /locus_tag="IW715_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1021822.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(25696..25887)
                     /locus_tag="IW715_001207"
     CDS             complement(25696..25887)
                     /locus_tag="IW715_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021823.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(25981..26367)
                     /locus_tag="IW715_001208"
     CDS             complement(25981..26367)
                     /locus_tag="IW715_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1021824.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         8989 a         3927 c         4897 g         8732 t
ORIGIN      
        1 aaatataaaa actagtacga aattaatttg taaattattt aagcttgata aaataagcaa
       61 tatcgatgat gtaaagtcca agatattaac aagaaaagta agcgaaccag taaagctaac
      121 ggcacaagat aaaaaaaatg gaattgttct ttatggtgat gatgcaaagt ataactataa
      181 ttgggatacc tcctcagaag ctgaggtatt aagtattcct actaccgata cttcagggga
      241 aaaagtcacc tttaaaataa attttataac ggcaatttct aagctggagt atgggaagca
      301 aaaaaagaga gataacaatg atcaatatct acctaaaaaa taacgaatta atgatgcata
      361 tgttaaagtt gtttttttga attaaacaac tcaatttatt cagttatatg ttcatcgcag
      421 caagtgaaca ttaatcgagt tataaaatta ataggagcta aaaatataaa taaagttcga
      481 tcagaattta tttttccaga agattttttt aactggtttt tttatcaatt cagtgaatgc
      541 aagcgactct taggtgagaa ttattactta aaaggaatcg gcggatttat ggggaatgtg
      601 gctgatgaac aaaatattat aaaaggaata tctgaacaaa catcagagct tacagtaact
      661 aaagcattta taagtaatgg tgaaagtttg aaaaatgtaa ctgcgcgaat tagaaaagaa
      721 aatgaagtag aaattgtttt tggaattgac catgaatgta attcagtgat atatgtaaca
      781 caatcagcaa atttaattct ttttgagaat ggttgcaagg acgattttat ggtggtctat
      841 ttgtacgata tactaattcc aaaacttcgt tccttatata tgtcttctga aagtattttt
      901 gtgagtgaac aaaagggatc tttctcgaaa aaaataggta ttgaagtaat tgaatcgatt
      961 atgtgtcata atcatattac gctagaagaa ttagaaattt tgatagggca aaaaaataat
     1021 aacacgaaaa tacaaatttc ttagaaatca ttttttattg agatatctag ataaatgatt
     1081 tattcttcaa tcaaccaact cgccaagctg aggtaatgtt ttgaggtaat gataacatac
     1141 aactttttat accctagtaa aactctataa acaaagactt attaatttcg catgaaatac
     1201 attcctgtaa aatgcaataa aatagagttt tgtttaacca ggtctcaaaa aggctcgtaa
     1261 agcatctcaa ttctctaaac gttaatatta tttatatatc aatgtttcaa ggcacttttc
     1321 aattgtttga aaagtgtctt tttcttgttt aagaacacat ttaagaacac atactaatca
     1381 catttattca atcctctctg aaaggcttca aaaacagttt tatctactac atctggtgta
     1441 tttacgtata ttctcgttgt agaaatatct gagtgtgtta aagcactaga tatttgattc
     1501 atatttgctc cgccttgtct agctatagtt gcataggtat gacgaagctt atgtggagat
     1561 aaatgagcca attcaggatg tcgtctttta attgaattga ttcgatagtt taggtaatca
     1621 gcgtgaacag ggcaatttac ttctccggat ggtttagtat aggtaaagag atactgatct
     1681 ggtgtttgat taatttttaa tctcaacaat tgctcagctt gcactgattt ccatttgctt
     1741 agtaatgcca ttacaatttc gggtacttga attacggtat cctttcgacc ttttgtaaat
     1801 ttcttttttt gatatttatc caaagcatgt ttaaggcgaa ccgtttgttt ctcaaagtcg
     1861 atatatttcc attgcaaagc atacgtctca ctttttctat caccaagata tagggtaagc
     1921 atgaaaagag tatagtcatg aagagtaagc aagtgattat tcaagtcagt gtttactgct
     1981 tctatccaag caataagctc ttctgacgat aatgcttgat taccttcccg tatccttctt
     2041 tcttctaaag caattttttt aggtgcagtg atacattgaa taattttggt agttcggtta
     2101 aattcaatat aatttagtat ttctgcaata tcaaacatag aacggacata tcctttgata
     2161 atttttatat ttgcgtattc tttcgatttt tttgttaatt catctgatat tatctcagta
     2221 tttgaattta gatagttcat tgcatactca ccaaacattg gaaggatgtg caatcggaat
     2281 atatctttag tattactaat tgttacgtaa cttggagctc tttttgatct agtagtctga
     2341 cctagttcat atctaggaag ccattttgat tcatagaatt ccttaaattt tatttttcct
     2401 tttaattcta gtgaatttgc attctcctct cttaaaactg tttcaatttt ccttttcatt
     2461 gcattttcgg ctttaatagc caattgccta gttggaaata ctttttgata cttttctgat
     2521 gtaattccag tgatttctct gaattttttt ggatacctca gattagtttt atatttccca
     2581 tttgaaagtt ttgtaggcaa aattcctcac tcccttaaaa agtaaggcag tatatttcgc
     2641 tattcttagt atatgcggcg agaagaaatt ttcaaattgt tttaaatgta ttcataaaat
     2701 ctttgatatc ctctatgtca aagcgaacta taccgtcaat tctgattact ggaaaattat
     2761 atatacgaat ccacttatct aaagtattat tatcaattcc tagaaactca caggtttgtt
     2821 ttttattcaa gtagactctc aattttataa aatcatctcc taaaaaagat tagaattttt
     2881 gaattgcttt gggtaagcac ataggaattt cacctctccc cagtttacgg cgagccttac
     2941 ggaagtatca ttatttgctg ctattgtcat ggcattttgt tttcccagaa caaaaagaaa
     3001 tctctaagta tttgtcgcta atcaggtcat ggcgtacttg agtttctagc tcgaatagca
     3061 gggatcgtta ttcaaaaatt gtatttaatt gtcaatgatc aaaaataggt tttttcctat
     3121 tcactattaa taacgataaa catgtataaa aagacacact acgaaaaaag ttaaatggag
     3181 tttactgatt ttcatatttg tatatggaac agacatagtc ttgaataatt tttataaatg
     3241 ttttgatgct tatcaactta ttcaacccgc tatccacttt aagtataatt tttccatacg
     3301 ccaataaagt agttttagga gcatgttctc atttgagaac gctccttttt tatatatttt
     3361 gaaagctact tactatacgc gtagtttcaa atagttttgt cattctaatc ttcagcaaca
     3421 gaaaaaaatc taaattataa aatttagggt tgactaaaat ataatttttg tataccatat
     3481 gatcgtgata atcattatca tttagaagga gaaagttatg ttagaagtga aaaagcttaa
     3541 tgtttgttat aacgattttt tagcattgaa cgagatttct ttaaaaatac aagaaggtgc
     3601 tttaacaggt attattggtc caaatggtgc tggaaaatct acattgctta aagctatgtt
     3661 aaatattatc cctcatcagg gagaggtctc cattaataat gaagatatca ataaaaaact
     3721 ggtgaagatt gcatatgtag agcaaaaagc tgatattgat tttacttttc caataaaagt
     3781 aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa
     3841 tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc gatatgggaa aatattctaa
     3901 tcatcaaata ggagaattat ctggaggaca atttcaacgt gtacttttag caagatgtct
     3961 tgctcaaaat gcggatttca tatttttaga tgaaccattt gtagggatag acttagttag
     4021 tgaacgaata attatggata cgttaaaaga attgaaacat cagggaaaaa cagtgctaat
     4081 tgtgcatcat gatttaagca aggtaaagaa gtattttgat aatattattc ttttgaatcg
     4141 aaatctaatt gctcacggaa gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc
     4201 ctatggagat actatattta ttggagagac taactatata gagtcaagta aaaattagcc
     4261 aaatcaaaaa atattttata acagataatt ccgaaaaaag ggtacaacaa ataagcttct
     4321 taccattagt ttttttgaat gttcaatagc taatcagaaa caattatata aggctcgttt
     4381 ttagtcaaaa tagcatgaat cacgtaacaa agcttacgag aaaccgcacc taaggcagtt
     4441 agatgatgtt ttccttcgga aacttttttc tggtaaaacg cactaaaaac aggatcacat
     4501 tgagaagcta ctaaagccgc cgaaaacaag gcttttcgca aatatggaga ccctcgttta
     4561 ctcataacat tatgcgttgc ctcatattgc cctgattgtg tcacggaagc gtctatccca
     4621 gcgtaagcaa cgagtttttt cgggttagaa aatttttgaa tatctccgat ctcaccaaga
     4681 atagtcgcac cgtttatagg accaattcca ggaattgtga gaataacaga atttaaagtg
     4741 tccattatgt gcttgatttc tctttccgtt tcctttattt ggctttcaat gaattttaat
     4801 tgttcaatca ttgaacgtag ttggaaggtg aacgcttctt gagcaaattt tactccaaag
     4861 gagttagaag ctgcttgctt caattggtcc gctttttctg ccttgattcg gttcctgctt
     4921 aactcagcta agtattgggc caaagtttct gaggaaatgt tttctaagtc gatcggggca
     4981 ctaaacgcca gcaataactc tttagaagct tttccgaaaa cgccctgctt ggtaaaaatc
     5041 gtcgcgtatt ctggaaagac ttgatccaat acggctataa tttttctttt gaagtcgctc
     5101 gctgtaccta ctaaataagt tcggtaacga gaaagttgtt tcaaagaaaa aacattttca
     5161 tcggataaga ccgtttcgac aaatgagcca tagcgcatta agtcagcaat caaaacagaa
     5221 tcaataatat cgttttttcg tttccgtatt tctgtacctt tacgccatcc gtctgtttga
     5281 aggggattca caacgtgaat caaataatct gattcgtgaa ggtatgaaaa gatggacagc
     5341 caatagtgcc ccgtggcttc catccctata acaaagtcac tgggtgctcc ggaatagtcg
     5401 accattcgct caattaatga ctctgctcct tcagtggtgt taggaaatga aaaacctttt
     5461 agtaatacct ttccttcctc gttcatgata gaagcaacgt gagtgcgttt accgatatca
     5521 atgcctaaaa agtacatgta tatctgacct ttcaaaaaaa gaattttgga ttgaaatcca
     5581 ctcaactgaa aagcgtcact accttgtacg agatacggag taaagtaagg actttcaaca
     5641 tctaactcat acgcaatcca cttatcagag agaggagcca gtctctcgag tgcgggttca
     5701 atggcccaag gggggaacgg cttagcctct caatccaata aatatattat ctcaaaagaa
     5761 atgagttaat accaaattaa aataacgatg gttcataggg ttatccaatc aaaaccggaa
     5821 ggttaaaacc ctaactctaa tatacaaggg ggaataatat gcttcaaaat tttattaatg
     5881 gtctgtatga ctttcacttt ttacagaatg ctctaattac atctgtagtt atagggattg
     5941 tttctggaac agtaggttgt tttattattt taagaggaat gtctcttatg ggtgatgcta
     6001 tttctcatgc tgttttgcca ggagtagcaa tttcttatat tttaggtatt aattttttta
     6061 ttggtgcgat tgtatttggt ctattagctt ctatcattat tacttttata aaaagcaata
     6121 gtgtaataaa aggagataca gcaattggta ttactttcag ttctttttta gcattaggag
     6181 ttatccttat aggagttgca aatagctcaa cggatttatt tcatatctta tttggaaata
     6241 ttttagctgt acaagatatt gataagtgga ttacaattgg cgtttctgtt ttagtgttgg
     6301 ttacagttgt tctatttttt aaagaattat taatcacttc ttttgatcca ttaatggcaa
     6361 aagcaatggg gatgaatgtc agtttttatc attatctatt aatgattcta ttaactttag
     6421 tagcagtaac agctatgcaa agtgtgggaa ctatattaat tgttgcaatg ttaattacac
     6481 cagctgctac agcatattta tatgcaaaca gcttaaaaac tatgatattt atttcggcga
     6541 cagtcggagc gtgctcatca ttgctaggtt tgttcattgg ttatagtttt aacatagcag
     6601 caggatcaag tattgtttta acagcggcat tcttatttgt aattagtttt ctaatctcac
     6661 caaaacaaaa atttttaaga aagaaggaga gaaagttatg atgagaaaat ggaaagcagt
     6721 actgggaagt ctggggattt tgattgctct ttttatattc ggggcatgtt caacaaatag
     6781 taaagacaaa gatacagtgg cttcgaacga aaaattaaag gtagtagtta ctaattcgat
     6841 tttagcagat attactgaaa atatagcaaa agataaaatt gatttacaca gtatcgtacc
     6901 tattgggaaa gatccccacg aatatgaacc tttgcctgaa gatgttcaaa aaacttcaaa
     6961 agcagatttg attttttata acggtgttaa cttggaaact ggaggaaatg cttggtttac
     7021 aaaattagta aaaaatgcga acaaagagga aaacaaagac tattttgcag caagtgatgg
     7081 catagatgtt atttacttag agggtcagag tgagaaaggg aaggaagatc cccatgcttg
     7141 gttaaattta gaaaacggta ttatttacgc taaaaatatt gaaaaacagt tagcggaaaa
     7201 agatcctgat aataaaaaat tctataaaga aaatctagat aagtatattg aaaaattgga
     7261 ttctctagac aaagaagcta aatctaaatt tgcttcaatt ccgaatgata aaaaaatgat
     7321 tgttacaagt gaaggatgct ttaaatattt ctcgaaagcg tataatgtgc cttctgctta
     7381 catttgggaa atcaacactg aagaagaagg aacaccagat caaataaaac acttagttga
     7441 aaaattacgc acaacaaaag ttccctcctt attcgtagaa agcagtgtgg acgatagacc
     7501 aatgaaaaca gtatcaaaag ataccaatat tcctatctat tcaacgattt ttactgattc
     7561 aattgcagaa aaaggacaag atggtgatag ttactatgcg atgatgaaat ggaacctgga
     7621 taaaattgct gaaggccttt cgaaataatt tggagggtag gtatggtaca aattaaaacg
     7681 actactatac taatttattt actagcaatt caggaattat caaaaagaaa aaagaaatta
     7741 aaaaataatg atttagcaaa agttctaagt gttagtccag catctgtaag tgagatgctg
     7801 gctaaactta gatatgagga ctatttagat ataggatttc aattaactga taaagggaaa
     7861 aaatttattg acgattacaa aaaaggtttt aagtaaaaga ctttattttt agggaggata
     7921 agaaaatggc tattggagtg attatgatag gacttaccct aatagctgga tataattttt
     7981 ttaaatcttt ctcggattat tttagaaaag gtgaatgatt tagatgagtc tattaattct
     8041 tgtaggattg ctttttgtat cagttatttt gctctttctc attagtttag ctctaaggaa
     8101 tgagttcagt aatagacgtt aacgattgaa acttcaagtc ctgtgtaaga taatatagtt
     8161 aattcatctt attaggagga agtacccata gaaaccagag attatattac aactcaccaa
     8221 aaattaatag aaagtggact aagccatttt gaaaaagaag gatttcagca aactaagata
     8281 aggcaactgt gcttgaccgc aggcgtaact acgggtgctt tttataaaca ttttacttct
     8341 aaggaagcgt tatttgaaga aattattcag ccgcatatta atgcgcttat taatatgtat
     8401 aatgaagagt ttaccaaata cctcttaaac ttagaaaata tagatataga gaaaagttgg
     8461 agagagaact tcaatgaatt agatccattt attaattata tttttgagaa taaacgtata
     8521 ttcgatttgg ttttattcca atcagctgga accaaatatc aagatattgt tgaaaaaatt
     8581 acatcatttg tgacaaaaaa gacagtctgt tgtctagata agttaaagac tatcggaatg
     8641 atcaatcaaa atttagtctt taatgaagaa gaaatccact tctacattta ctcattctac
     8701 gctacattct atgatatatt gaagcatagc tattcaaaag aaaagactat gctattaacc
     8761 agaagattgt atcaattttc tgttcctggg tggattgaat tactgagtta attaaaaaaa
     8821 agcttgcatt gagaattgtt ctcagttatg ctttttgata agatcgcact gttatctgaa
     8881 taacggtgca tttttgtcag cttaagataa cagtgcgatc ttattttttg gaggtgaaag
     8941 agtgtcaaaa atcaatccgt taaccttgat tactttaaat attttttttc cggtcattat
     9001 ttttttagga aagggaaata tatttcaagt ggggtgtttc attattgcta cagttttagt
     9061 attgatgttt cgtaaaataa agacatgtat ttatttcatc ttgatttatt cagttctgtt
     9121 tttagtaatg aaaattattg atgctatcaa gatagagttt tttactatat tttttgggac
     9181 atccttatat attgtatttc gaatgttgcc ggtcgtaatg atctcatggt tgttggttag
     9241 tgtctatact agcaatgaat tattatcctc tttagaaaaa atacatttac cgaaaaaaat
     9301 aatgttaagt attacagtag tgatcagatt ctttccgact tatcgttctg aaataagaat
     9361 gattaaagaa tctttaaaaa tgagaaacat tcgcttgtct ttttttcagc ctctgcaata
     9421 tttggaattt ctgttagttc ccgttcttat acgtgcaact atgattgctg aagaaatgac
     9481 cgctaatgca ataacaaagg ggatagaatc tcctgtgaaa agaagttctt actataaaac
     9541 aaacatgagc ttattagact atatcatcct gttttccgtt actatttttt tcttaagtat
     9601 tttgatttga ggtgaaagaa ttgtttgaat ttaacaatgt ttctttttca tataaggatg
     9661 ttaaggaaca agcgcttgtt gaccttaatt tttcaataaa aaaaggcagt cttacattga
     9721 ttactggttc ttctgggagt gggaaatcca cactattaaa gttaatgaat ggacttattc
     9781 caagacttta tgacggaaaa ttggttgggg aaattttatt tgaaaataac tcgctacaat
     9841 ccttaacaca agaaagtata tcaaaaaata ttggctacgt aagtcaaaat cctagaggac
     9901 aattctttac aacaaataca acgagtgagt tagtattttc gatggaaaac tttggattta
     9961 caaaagagca tatggataaa agaatcaatg aattagctga attattagat ctttcaaata
    10021 tattagataa aaacattttt tcgctatcaa gtggtgaacg gcagaaaatt tctattggat
    10081 gtagtttatc gctaaactct aaattaatta tattggatga accgtcttca aatttggact
    10141 tcaaagcgac taagaaactt gctgacttac tttgtgattt aaaaaataaa ggatttacta
    10201 ttgttatagc tgaacatcgt ttttactatt tgaaagaatt aattgatcaa gttctatttg
    10261 ttaaaaatag aactgtaaaa actacaacta ttcagttgtt aaaaactgaa aacaataacg
    10321 cattaaggaa atttgacatt tttaatagtt cgattgaagt tccctatatc gaaaaggtaa
    10381 gtgcagatgt attaaaagtt gaagaaatta cttatgaaaa tattctaaaa gacataaatt
    10441 ttgatgtcaa agttggagat gttattggtt tggtagggaa aaacggtgtg gggaaaacaa
    10501 ccttactaag aatactcatg aagattattc gccctaccaa aggaaagata atcgaaaata
    10561 aaaaaaattt gtcatcgccg tttttagtaa tgcaagaaat ggattaccaa tttttcacag
    10621 aatcagtgag gagcgagtta gccctgggga atgaaatagt tagtaaagat cagcaagaaa
    10681 aaattttaaa aaaaatggaa ctttataaaa tgaaagatca attaccgttt gatttatccg
    10741 gtggagaaaa acaaagatta ttggtttcta tagcttcatt gtcaaaaact aatttatttc
    10801 tatttgatga acctacaagt gggttggatt atatcaatat ggaaaggatt gctgacctta
    10861 ttaaagactt aaaagaaaag ggtgcagtag ttatagccac tcatgatccg gaatttcttt
    10921 ataaaacatg caatagaatt atttatttaa aaaatggtgg aataaaaaaa gatataaaac
    10981 ttagagtaga agattcaact ttgatagaac agatttttaa tgacataacg aaatagaatt
    11041 gaggagaata tatatgaaaa caaaagatta tatttttatt ggtttagcta cagttatttc
    11101 cctagtaatt tacttttttt caataatggt tagtagtatt ggtggggcgt ttggtcacag
    11161 catatcccca gggatttttg gattattatc tggaatcata tttgtttata ttagctataa
    11221 ctaccctaga aaaggaattt ttactattta cacaattgtt ttattgttat tttttacgtt
    11281 aatgggcggg gcgtacttac cttggtttat cagttcgatt agtatggcga tcttagctga
    11341 tattattcta tctgtatttg gatatgatcg agcaattcct caagttgtgt cttgggttct
    11401 aatgcaacta ggcagtgctg ccggacagtg gattcccata tggttcttta cagatcgttt
    11461 tcgtcaagac tggatagata gaggacaatc tgctgcaaca atggatgcaa tgatccacta
    11521 cgcggtgggt atatggggaa ttatttcagt gttagttgta gcaagtttat ctatgattgg
    11581 tgtattgatt ggaagaaaag tcttaaaaaa atacaaaaaa tagaacgtag tttaccttct
    11641 atctgtttgt acatctctaa tttacgctat tacggaaagc attaacagtt gtaaacgaat
    11701 aagagaagaa attgaatgcg ggggaggaaa aaatgaaagt atacaaaaaa ttatttagtt
    11761 atgtccctaa aatgaaatac tacggatggt tagccacttt attttctgat ttttctgtcg
    11821 tattaacagt ctatggatat tacagtattt acaaatttct tcagtctcta attattgtag
    11881 gagatgaata tttagctaag agttatgcgg ttcaaactgt tgcttggtta actttaggag
    11941 ctttatttta tattttttct ggcttatttt ctcatatcct tgggtttcgc ttagagacta
    12001 atttgagaaa aaagggaatt agcggtttaa cagaagcaag ttttcgtttt tttgatttaa
    12061 attcttcggg atatattcga aagacaattg atgacaacgc ggctaaaaca catatggcaa
    12121 ttgctcactt gattccagat agtgctcaag ccatagtaac acctgtttta tcagttgcct
    12181 taggcttttt cattaacttt aaaattggat tggttctaat cgccatgtta gtaattggtg
    12241 tattcttgtt aaagaaaatg acagagaata caaattttat tcaaaaatat caagaatcgt
    12301 tagatgtgtt aagctctgaa acggtagaat atatccgcgg aattcaagtt ataaagatat
    12361 ttggtgctaa agtaacatca tttaaagcct tgaataaagc aattaacgat tatgctaagt
    12421 ttgcatataa gtattctttg agtgggaaaa caccttttgt tctttttcaa tggctatttt
    12481 ttggcatagt ttgcattttg atagttccta taaccttttt tataacttct cttaccactc
    12541 ccaagatctt agctgttgac ttgttgatgt tgtttttttt aagtggaatt atttttgttt
    12601 ctttcatgag aataatgtat gtaagtatgc acttgtttga agcaagatat gcagtcgaaa
    12661 cactagactc cctttattta gaaatgaagc aagaccaact aaaatatggg aacgagacac
    12721 agattaaaaa ttgtgacatt gcatttgaaa atgtcagctt ttcatacaaa gaagagtgtg
    12781 ttttagaaaa tttcaactta gttttagaac aaaaaagaat ttatgcatta gtaggaaaat
    12841 caggaagcgg aaagagtacg atagctaaac taatttcagg tttctacaat gtggataagg
    12901 gacagattaa aattggtggt aagcctttaa atcactattc aaaagacaca attattaaca
    12961 caattgcttt tgtctttcaa gatacgaaac tattcaatca gagtatttac gataacgttt
    13021 ctatagcaaa tcctcaagcg ccagcaagta aagtatttga cgcgctaaga ttagccggtt
    13081 gcgaatcgat tttagaaaaa tttcccgaaa gagagcatac cttaattggt agtaaaggag
    13141 tttatctgtc aggaggagaa aaacaacgta tagctattgc cagagcaatt ttaaaagatg
    13201 caaagattgt tattatggat gaagccagtg catctattga tccagataat gagtatgaac
    13261 tacaaaaggc ttttcaatat ttgatgaaag aaaaaacagt tattatgata gctcatcgat
    13321 tatccagcat taaatctgta gatgaaattc ttgtcttgtc agaaggaaaa atagtagaac
    13381 gtggaaatca taatgaatta atggctaaac atcgagtgta taaacaattg gtcactaatt
    13441 atgagaatgc aaatgattgg aggcttgaca atgaaaaatt tttataaaaa aaaatttgct
    13501 ttaactgatc agggcgcaga agctttaact aaagcttcta tttctagttt ttttgtatac
    13561 tgcattaata tggtaccagc attcattata atgatgctga tagacgaatt agttttagaa
    13621 aatgcaaagc cccgttggct ttattttgca gtatcgtttg ttactttgct tttcatgtat
    13681 tggttgttag atagggagta tgaaaactta tataatagta cttataaaga aagtgcgcat
    13741 ttaagagtgc aaattgcaga cgatttgtca aatttaccat tatcctattt ttcaaaacat
    13801 aatttatcag atttatctca aactatcatg tctgacgttg aaggtattga gcatgcgatg
    13861 agtcatgcaa tacctaaagc cggcggtatg gctctgtttt tcccttttat ttcagtgatg
    13921 cttttggttg gtaatgtcaa aatgggatta gctgttattt tgccaacgtt atttagtttt
    13981 gtcttaatct tgttatcaaa gaaatcccaa acgaaagcca atactaaata ttacgatact
    14041 ttgagagaaa actcggaaga atttcaagaa actattgaat tgcagcaaga gattaatagc
    14101 tttaatctat ctaaaaaagt tcaagacaga cttttcaaaa aaatggaaga gagtgaaagg
    14161 attcatttaa aggtagaatt aagtactttt tcagtcatgg ccttatcctc tattttctca
    14221 tatgttagtt tagcagtagt cattctagta ggtgttcact tactgttaac gggtgaagtg
    14281 actatactct acgtcgttgg ttacttacta gccgcaataa aaataaagga ttccttcgat
    14341 tctatgaaag aagcagtgct tgaaatattt tatttagctc ctaaaataca gcgaattaga
    14401 gctatgaagg aaaccagtat ccaagaggga tcagatagtc ctctgaaatc ttttgacatt
    14461 gagttgcgag atgtctcttt ctcatatgat aacaataccc caattttaga tcatatctct
    14521 tttacggcta aacaaggaga agtgactgcg ttagtaggtg ctagtggttc cgggaaaact
    14581 agtatcttga aattagtgtc tagactgtat gattatgatg aaggctgtat tttaattgat
    14641 gggtatgata ttaaacgtgt atctccagct tctttgttta gcaaaatcgc tattgttttt
    14701 caagaggtaa ctttgtttaa tacaagtatt ttggaaaata tacgtattgg aaatagtcaa
    14761 gcgtcagatg aggaagtgaa aaaagcagct cgtttagcta actgcgaaga ctttattgaa
    14821 aaactacctg acggttatca tacattggtc ggagaaaacg gaagttcact ttctggagga
    14881 gagcgacaac gcctatccat agcacgggct tttttgaaaa atgcgccaat tttaatttta
    14941 gatgaaatta ctgccagttt agatgcggaa aatgaaaaac gtattcagga aagtttaaat
    15001 cgtttaatac aagataagac agtactaatt atatcgcatc gcttgaagtc tattgaaaag
    15061 gtaaataaaa ttgtagtgat ggatcaagga aaactagttg atcaaggtac tcataatgag
    15121 ttgtatcgaa ggtcagaaat atacaaaaat ctcataaaga aaactaaact atctgagaaa
    15181 ttcgtctatg aaaaagaagc tcaatcacga tgatgaaata ggctttaaat taacctacaa
    15241 tttttgggat atagttataa ttctttttca aaaagaataa ttttattaaa cagcatctaa
    15301 ttttgaatac acgaaagaat cagtatgatt gaattagttc acttaatgtc tattcttata
    15361 atataaggaa taatcaaagt tttaaaaaat tggcgataaa ccagttaaac ataaaagatt
    15421 gaataaaaag ttatagtttc tttcatattg ccgttttaag tagaggaaaa gctacttaaa
    15481 acggtatttg tgtttgattg gactaataaa agtgaattct tatttttcag agcctaaact
    15541 gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt ttcaaatagt
    15601 tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca actcttgttg
    15661 gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat caaaataaaa
    15721 ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa acaaatggcg
    15781 atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga tcgcaccgct
    15841 ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga tcagacggat
    15901 ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt tagagaactt
    15961 gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga ggaaatatca
    16021 tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga actcatcaaa
    16081 ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga cgtagccatc
    16141 atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct tcaaaatagt
    16201 ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc tttagttgaa
    16261 gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg cactttttat
    16321 cgttcgatgg tagagagagg aattattact gaaacgggtt taccgaccca acaggcaatt
    16381 gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga tgatttttta
    16441 agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat cgatggttat
    16501 tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc tggcgagtta
    16561 acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt attgaaggag
    16621 gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa cgtctgtatc
    16681 tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag tattcgccaa
    16741 aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa aaggtgacaa
    16801 aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa caacgctaat
    16861 caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa ttgaaaatga
    16921 gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac gggtgttaca
    16981 actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa tattgatttc
    17041 tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca ttgaaactac
    17101 aggtatagca gatccccaac caatcattca aacactttta acagcgcctc aactaagagg
    17161 aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg aacttattga
    17221 aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg tgtctagaaa
    17281 acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa gtattaaccc
    17341 tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg atttttataa
    17401 tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa atcactataa
    17461 tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc tcttaacttc
    17521 agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga ttttaaccaa
    17581 ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg attttatagt
    17641 tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac aaaaagacca
    17701 aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac agagttttga
    17761 taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt tctaatggag
    17821 aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa aactagaaaa
    17881 agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt agcataatta
    17941 aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca gctctaggat
    18001 tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa gatggaaaat
    18061 tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc gtcggcgatg
    18121 aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat gagccctcag
    18181 ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac gaaaacatgg
    18241 aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat gtagtagaag
    18301 gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat cacggagaag
    18361 aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg gcaattaagg
    18421 aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca aaagtatttg
    18481 agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac tataccacta
    18541 gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt ggatatttag
    18601 ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa gaagagccat
    18661 cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc aattatattt
    18721 actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa gctgggataa
    18781 aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat aatggtgaag
    18841 attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact atggttgctg
    18901 gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct aatgggtact
    18961 ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa tggcaatctg
    19021 tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa gcaaaaatca
    19081 ataaagacat gacagctgcc gagtacaaag attattatac tactggctat aagacagata
    19141 ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt gaacatcatc
    19201 aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga aaccgtggtg
    19261 ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat attcaattta
    19321 gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt ggtggagaaa
    19381 gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca gcttcattgt
    19441 ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt ggaggaaata
    19501 catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt atcggcgtta
    19561 tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg ctgcaacttt
    19621 acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt gttgttttat
    19681 caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct tggccaatta
    19741 attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc tttttattat
    19801 gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata aggtgttctc
    19861 caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt tatcgttgag
    19921 ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag tctattcgta
    19981 ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag ggcatattat
    20041 tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct ggagtgatcg
    20101 ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg ctcacataag
    20161 cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt ctgtctgtca
    20221 aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc caatgaccaa
    20281 aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta aggcaaatct
    20341 ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg tgcaatgaac
    20401 ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca ttgcaaattg
    20461 cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc aaacgattcg
    20521 agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt gaccaaactc
    20581 acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca taattttttt
    20641 ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta aaatagtaaa
    20701 cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt cgtgaagcag
    20761 aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa ttattttcct
    20821 tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact catttttatg
    20881 tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa aaaaatacct
    20941 attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa tcatattctg
    21001 tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga agtaaatgtt
    21061 gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact tgtttcgatg
    21121 ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact agatcaatta
    21181 gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag caaaccaatt
    21241 ccaattattc aagcaattat attagctaaa aaccctttag acattgattt gacccaacga
    21301 ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga taatttcgaa
    21361 acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga agaacaggat
    21421 attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt aaataatagt
    21481 gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa gttacagtcc
    21541 aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat tctaaataca
    21601 aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga gttagagctt
    21661 ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt tatctatcga
    21721 aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa ttttcctggc
    21781 aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt ttcgacgatc
    21841 tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt agctagttta
    21901 cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga agcctggcat
    21961 ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat ggatcaacaa
    22021 tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga taatttccga
    22081 aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc gttaatgaaa
    22141 tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat tttgattatt
    22201 acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac aactgaagat
    22261 gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt cgatttggat
    22321 aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa tattgttgag
    22381 attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat cgcggttaga
    22441 actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa gttagttatt
    22501 agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa tagatgagga
    22561 ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat taagtgtgtt
    22621 aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct ttaacgggac
    22681 ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg tcaatgagtg
    22741 ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag ctatcttgtc
    22801 tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt tcttaaaata
    22861 ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat agccataggc
    22921 aattcgtttt agtactttga tatggttatt caaacattct atagggccgt tggaataggg
    22981 aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga acactggttg
    23041 gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt caggaacatc
    23101 ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct ttaaggaatc
    23161 atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc gaaatgacgg
    23221 acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta gtttccagta
    23281 tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta cttgaattcg
    23341 gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg cgacgattcg
    23401 ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac
    23461 tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc gtaaaggatt
    23521 gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa
    23581 gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca ccacttctgg
    23641 taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc gaatgacagt
    23701 ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt cgcttaatag
    23761 ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac taactgaatc
    23821 ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt taatccgcag
    23881 aatggtcttg tagttactga catcattcaa taagatggat acttttttcc agccctattt
    23941 aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt gataggtcaa
    24001 tgtgccatca aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa aggcatcttc
    24061 atgaaagata agatttaggt ctagaatatc tagtaactct ttggtaaact ctgaatagga
    24121 catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga ttttaaggtg
    24181 tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt caccaacact
    24241 agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat cataaatttt
    24301 gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa caccttcatc
    24361 tctattttac ttttatattt gttggagtcg attaattctc atttgattga tcgatgaaac
    24421 gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcactgaatg tgtgatgtgg
    24481 tggtcgttca acagctcttg ataaataaaa gaccgatgct ggctacctct atcggaatga
    24541 attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat ctcttttttc
    24601 atgtgtgaat ctaccatata gtaacgtaga atacgagttg ctaagtcaat ataggtggac
    24661 aaatagaggc gaccgtcatt acatggaatg taagtaatat ccatcaccca aacagcatca
    24721 atttggttct gttcaaatta tcgattcaac atttttttac catttctttg attgtttttt
    24781 ctctaccatg agaagatttg cgtcgatgaa atcctcttgt gcataaattc atttcacgca
    24841 taagaacagc tacaacacgt ttattgattt cgattccttc atcgtaaagt ttttgagtga
    24901 tccctggaga tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct cttttagtcg
    24961 tttattttcc tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt ttccttgtcc
    25021 agcaaaagca tttttcccgt atcgacgata ttctgaaacc catcgggaaa ttgttggacg
    25081 agagatattg tattcttcag caattttctt ggtagttact tttttctcaa ggatatcctg
    25141 tgaaatcttt agtttaaatt tacgatcata tgtttttctc aatagagttc attccttccg
    25201 gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca acttagtatc agggtttgtc
    25261 aactaaactg tggaagttga tagataagag tttttaacaa ccaaaattct cttgactatt
    25321 tttgaaatca gacaaatttt gattggacac atagttgaat aaatctaccg tcgttacgga
    25381 aggtaaatcg catgcttttc aattctagag gagaagtcct cgttgaagta ccatagccca
    25441 gttctggatt cgacctcctg cccacttcat acgtaaataa agtaatttta ggagtgagac
    25501 tgtgacaaaa gttattttta agctaaaaac gaattaaaaa acgttgataa ttaaaatcat
    25561 tcgttttaga ttctatgaat cattatagaa cgctgtaaaa tcgatacttt ataattataa
    25621 agaagatagt ttcaaattta ttgttcgaaa tttgaaacta tctttttact tatgtcacag
    25681 actcccttag ttgagttatt ctttaatttc gaatttcgag atagctagat atattgcaag
    25741 catacattct tgataacaat cttgatcata ttttccagtt ctccaggatt gcttatgaat
    25801 caaagagcta aatcttttga gaattgcctc aacagcttct tcatttccag cctttgctaa
    25861 aatgaaatct tcttttaaag aattcataaa tctaccttct tcccatggac ttataatcta
    25921 tttctttggg aaggaggaga acgagcaaag aagcatacaa aattagttat tatgcagata
    25981 ttaaaccagc attttttgaa tttttttgta aagtcgatgt tttaaatttg tcgcaccctg
    26041 tcttgtaatc cccagaacct ttccaatttc tttatcagtt ttatcaaaga taaatttttc
    26101 gataagaaaa atttgttcag gttctttcaa caaaggaagg atttttgcta attttatatc
    26161 ttcaacaagg agagtcacat ttaaaacatt aatcggttgt ttaaatttta tagtatcttt
    26221 agtaaacaaa tatggtggga gttgatcaag ttgttcatgt ttttgaattt cttcttgttt
    26281 tttataataa gtattagcgg tgttcaaaat gaccttttca aagaatcgaa tcaactgggc
    26341 atttacgcgt tcgtcgttat ctgacatatt gcacacctcc tccattaaga tttaaaggga
    26401 aaagaggcaa aaaacacgaa aagaagctaa tgcaattatg ctgcgctgcg aataacttct
    26461 ataagctcac atgcattgat aggactgctg atgtttattt tcaaaaatgt ttttttagtt
    26521 gataatggat agttatcttt atact
//
LOCUS       DACEMK010000034        26352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEMK010000034 DACEMK010000000
VERSION     DACEMK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.034"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1228)
                     /locus_tag="IW715_001209"
     CDS             complement(98..1228)
                     /locus_tag="IW715_001209"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1021825.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IW715_001210"
     CDS             complement(1877..3079)
                     /locus_tag="IW715_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1021826.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IW715_001211"
     CDS             complement(3139..3342)
                     /locus_tag="IW715_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1021827.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IW715_001212"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IW715_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IW715_001213"
     CDS             4879..5691
                     /locus_tag="IW715_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021828.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IW715_001214"
     CDS             complement(6715..6867)
                     /locus_tag="IW715_001214"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021829.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IW715_001215"
     CDS             complement(7058..7936)
                     /locus_tag="IW715_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1021830.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IW715_001216"
     CDS             complement(7975..9093)
                     /locus_tag="IW715_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1021831.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IW715_001217"
     CDS             complement(9090..11189)
                     /locus_tag="IW715_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1021832.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IW715_001218"
     CDS             complement(11240..12061)
                     /locus_tag="IW715_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1021833.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IW715_001219"
     CDS             complement(12058..12867)
                     /locus_tag="IW715_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1021834.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IW715_001220"
     CDS             complement(12888..13382)
                     /locus_tag="IW715_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1021835.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IW715_001221"
     CDS             complement(13394..13882)
                     /locus_tag="IW715_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1021836.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IW715_001222"
     CDS             complement(13959..16655)
                     /locus_tag="IW715_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1021837.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IW715_001223"
     CDS             complement(17083..18156)
                     /locus_tag="IW715_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021838.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IW715_001224"
     CDS             complement(18153..18980)
                     /locus_tag="IW715_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021839.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IW715_001225"
     CDS             complement(18981..19787)
                     /locus_tag="IW715_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021840.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IW715_001226"
     CDS             complement(19799..20884)
                     /locus_tag="IW715_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021841.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IW715_001227"
     CDS             complement(20901..21443)
                     /locus_tag="IW715_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1021842.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IW715_001228"
     CDS             21701..22690
                     /locus_tag="IW715_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1021843.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IW715_001229"
     CDS             22687..23424
                     /locus_tag="IW715_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1021844.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IW715_001230"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IW715_001230"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1021845.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IW715_001231"
     CDS             23988..24620
                     /locus_tag="IW715_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1021846.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IW715_001232"
     CDS             24705..25352
                     /locus_tag="IW715_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1021847.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IW715_001233"
     CDS             25357..26340
                     /locus_tag="IW715_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1021848.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8059 a         5571 c         4466 g         8256 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatc
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacggtc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg at
//
LOCUS       DACEMK010000035        26289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEMK010000035 DACEMK010000000
VERSION     DACEMK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.035"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW715_001234"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW715_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1021849.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IW715_001235"
     CDS             complement(1551..2972)
                     /locus_tag="IW715_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1021850.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW715_001236"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW715_001236"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1021851.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IW715_001237"
     CDS             complement(5383..6639)
                     /locus_tag="IW715_001237"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1021852.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IW715_001238"
     CDS             7219..8520
                     /locus_tag="IW715_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1021853.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8613..10001)
                     /gene="ltrA"
                     /locus_tag="IW715_001239"
     CDS             complement(8613..10001)
                     /gene="ltrA"
                     /locus_tag="IW715_001239"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1021854.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(10488..11087)
                     /locus_tag="IW715_001240"
     CDS             complement(10488..11087)
                     /locus_tag="IW715_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1021855.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            11341..12222
                     /locus_tag="IW715_001241"
     CDS             11341..12222
                     /locus_tag="IW715_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021856.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            12235..13845
                     /locus_tag="IW715_001242"
                     /pseudo
     CDS             12235..13845
                     /locus_tag="IW715_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(13922..14581)
                     /locus_tag="IW715_001243"
     CDS             complement(13922..14581)
                     /locus_tag="IW715_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1021857.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(14864..15529)
                     /locus_tag="IW715_001244"
     CDS             complement(14864..15529)
                     /locus_tag="IW715_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021858.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(15676..16281)
                     /gene="dhaL"
                     /locus_tag="IW715_001245"
     CDS             complement(15676..16281)
                     /gene="dhaL"
                     /locus_tag="IW715_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1021859.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(16309..17292)
                     /gene="dhaK"
                     /locus_tag="IW715_001246"
     CDS             complement(16309..17292)
                     /gene="dhaK"
                     /locus_tag="IW715_001246"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1021860.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(17294..17689)
                     /gene="dhaM"
                     /locus_tag="IW715_001247"
     CDS             complement(17294..17689)
                     /gene="dhaM"
                     /locus_tag="IW715_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1021861.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            17843..18163
                     /locus_tag="IW715_001248"
     CDS             17843..18163
                     /locus_tag="IW715_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1021862.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(18275..19144)
                     /locus_tag="IW715_001249"
     CDS             complement(18275..19144)
                     /locus_tag="IW715_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1021863.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(19240..20544)
                     /locus_tag="IW715_001250"
     CDS             complement(19240..20544)
                     /locus_tag="IW715_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1021864.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(20541..21050)
                     /locus_tag="IW715_001251"
     CDS             complement(20541..21050)
                     /locus_tag="IW715_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021865.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(21225..23360)
                     /locus_tag="IW715_001252"
     CDS             complement(21225..23360)
                     /locus_tag="IW715_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1021866.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(23443..25098)
                     /locus_tag="IW715_001253"
     CDS             complement(23443..25098)
                     /locus_tag="IW715_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1021867.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(25100..25414)
                     /locus_tag="IW715_001254"
     CDS             complement(25100..25414)
                     /locus_tag="IW715_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1021868.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            25584..25844
                     /locus_tag="IW715_001255"
     CDS             25584..25844
                     /locus_tag="IW715_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1021869.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            25858..26172
                     /locus_tag="IW715_001256"
     CDS             25858..26172
                     /locus_tag="IW715_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1021870.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         7377 a         5497 c         4756 g         8659 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaatc gagtagaggg gaaagccaac tgaccttccg cccctctcac
     8581 accaccgtac gtacggaccc gtatacggcg gttcaataag ttgagtgtac gaactggtac
     8641 ttcaaggaga tgtcctcata tccttggcgg gccagttctt tgtctgttaa tgatcgatga
     8701 agaatatgac ttttggagac tgaccaaata ccttttcttg tatttgacca ttcatacgct
     8761 ttcgctttat cgatccctaa acgaatcaga ttttttcttt tggttcttgg attcttccat
     8821 tgtttccaaa tatattgtct gattcttcgc ttcaaccaac cgtttaactc cctcatgaat
     8881 tctttcatct caccaattcc atagtagttg atccaaccag tcattagttg cttgatttct
     8941 tttaaaataa gttggatact tcttccacgg tttcttttag tgagttgttt gagcttgctt
     9001 ttcacacgct gttttgcttt tgcgtgaggg cgaattttaa tacctttctt tgttggcaga
     9061 atgcagaatc caagaaactt tcgtttcaat ggacttccta ccgcactctt ttctcgattt
     9121 accgtcaact ttagttgttc ttcgagaaat tgaatggatt tcttcatcac acgctgtcct
     9181 gctcgtttgc ttctaacata gatattgcag tcatcggcgt atcgaacaaa tttatgtcct
     9241 agattttcta gcacttgatc aaattcgttc aggtagatgt tacttaacag gggagagaga
     9301 ttgcctcctt ggggcgttcc ttcttcagtt gttgtgatga gtccattttc cattacacca
     9361 ctcattaagt attgacggat cagttttagg acttgtttgt cctttacttt ccgctcaatg
     9421 aaatacatca gcttatcgtg atttacggtg tcaaagtagg ctttcatatc aatgtccacc
     9481 acatatcggt aaccttgttc ataataagat tttgctttct tcactgcgtc atgtgcgctg
     9541 cgatttgggc gaaacccata actattatct gagaattctg gttcaaatag cggttgtaac
     9601 acttgattga ttgcctgttg aagcatacga tcaataaccg tgggaattcc tagttttctt
     9661 acaccgccgt ttggtttcgg tatttctact cgcaaaacag gctgaggttt atagttccct
     9721 tggcgtaaat tggatagtaa ttcctcgcgg tgctctttca agtaaggaag cagttgatcg
     9781 acggtcatcc catctgcacc agccgcccct ttatttctga ctacttgaag atacgcttga
     9841 ttgagattat tccgatctag aattctccca aacagatttt ctacaccatc ttgtttcctc
     9901 tgttcaccgc aagaagtgct gcgcacttca tcataccctt cgtgttccac actagctctt
     9961 tgatgatagc caagttttct tgttttctgc ggtcgtcgca tcttctcaca cctcctaaat
    10021 tattcaagat tcttattgtt cagtccttcg taacggtcag tgtcgttact actatgacct
    10081 cggctgactt ctcatgattc accatcatgt cgccatgatc gttgtgctgg tttgttttca
    10141 ttcaacgccc acaacaccct catgagatct cccccggtaa gagtgaaaac tttcctccca
    10201 tgtaactgct agatttactg tataggtttc gggcagtatt ggactttgtc ttgtgatgaa
    10261 gacttatccg acctaattca gccttgtatc tagtttctgt tcgtcagttc aggagtttgc
    10321 gtccgacttc cttcagccac aacctcacga ttgccgcctt gtctttcgct aacagttcct
    10381 actgccaagc ctgtagtgga ctttcaccac caagttttca cccatgcagg gcgcacacca
    10441 aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta tttcttcttc
    10501 ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag cagttttgcg
    10561 tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat caagaactca
    10621 tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag atcaagcatg
    10681 atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt tttttctttt
    10741 tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt gagataacgg
    10801 attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac
    10861 gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata aatcagccct
    10921 tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt ctcagctacc
    10981 ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt
    11041 aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc
    11101 cgtaatttta aacttttata atatatatag ttcaaattta ttctgaggtt tattataact
    11161 atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg acagaaattt
    11221 ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg tataaaacct
    11281 ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag tggggttttt
    11341 atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt cgaagcttta
    11401 aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg accaaatggt
    11461 tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga cggcggaacg
    11521 gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga acgtctttct
    11581 tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat catcgatctc
    11641 ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat tcaacgtttc
    11701 tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc
    11761 ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc tacttctggt
    11821 ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa agaacagggc
    11881 aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc ggataaagtc
    11941 gtgatcatac gcgaaggaac actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg
    12001 actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc acaattgaac
    12061 ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga taacgaagct
    12121 ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga atctattcca
    12181 ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag cgaaatgact
    12241 gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga ctggaaaaag
    12301 atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa
    12361 gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa cccggcaatg
    12421 attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt aggagcgatg
    12481 tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc tgttttacac
    12541 gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat tcgttccttt
    12601 cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct gatcaatttg
    12661 cttttagcaa tatcgatcag ttgtacgatg atttttttcc aagcggattc gatcactgca
    12721 gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg gagtgccgtt
    12781 gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct gtctttagcg
    12841 tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc ctccctttcg
    12901 atgtggaaac ctttaggctg gtcttatttg acctatccat ttacaactaa caactgaatt
    12961 cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt tgttttagag
    13021 gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa
    13081 cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat gacaatcagt
    13141 tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg gaatatgcaa
    13201 cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg cgtctcaatt
    13261 gaaaaaaact ttacaagcac gatcatggtc gtcttgattt gtctagctgc tattttacca
    13321 attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa tcagctgttc
    13381 gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc tattctattt
    13441 gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt ggcttcgatg
    13501 aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa tttcctgcct
    13561 gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc tcgaatcggt
    13621 aaacttgctt atatctactt agcttactcc tttgcactca attactttgg cggaattttg
    13681 gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg gatgccaaac
    13741 gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc actgttcgtc
    13801 cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa aagcatgtac
    13861 aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt cattttttta
    13921 tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc tttcttttta
    13981 cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg gtcgtaccga
    14041 taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc cctgtcgctt
    14101 tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc atcaaaaaag
    14161 ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt ttcagactaa
    14221 gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg caaatttccg
    14281 ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac tggctctctc
    14341 cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta gcagataatt
    14401 gatacacttt catactgccg gaggccacga tcactaattg aggttctcca ttcggcgcaa
    14461 gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact ttttcttgat
    14521 cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga tcatgactca
    14581 taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc ccttattcca
    14641 actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc agcaaataga
    14701 gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa gtaattgatt
    14761 cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt cactcgcaag
    14821 ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga aatttttccg
    14881 cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa
    14941 cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt cggtctctca
    15001 tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac aaagctaagt
    15061 ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata tcgactgttt
    15121 tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt
    15181 cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga ttcaacaaaa
    15241 ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc caaaaatgac
    15301 tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg ccgtacatat
    15361 caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag tgttctttat
    15421 attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc tgtttgaatg
    15481 cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt gtttcgcctc
    15541 tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag aggttgtgaa
    15601 aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat gagcatcacc
    15661 gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa ataaaatggc
    15721 actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat aggaagctcg
    15781 tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt
    15841 taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca tcgttttatc
    15901 gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga tcacttcgcc
    15961 taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca tgttcatgaa
    16021 tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg ccatagagaa
    16081 tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt cttcataagc
    16141 tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg tatctagatc
    16201 actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc attcttggat
    16261 ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt accatgaaat
    16321 cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt
    16381 tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc ggaaaactac
    16441 attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga actgttccat
    16501 caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta aattcgagta
    16561 atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct gcatcttttc
    16621 tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat cttctcctag
    16681 ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta gaccgatcgt
    16741 cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg aagcaccgtt
    16801 tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc cgcgttttcc
    16861 agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac tttctacttc
    16921 aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat cccctgtata
    16981 attcttaacg atcagcaaaa ctccttttcc actatcggct tccttaatag ccgtctgaat
    17041 ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta acatcccttc
    17101 ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa ccaaaccaac
    17161 ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat gaacaagttc
    17221 tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg gatcattcat
    17281 gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct gttaagattt
    17341 gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct tccactaaag
    17401 gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct tttgccagtt
    17461 ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct gcatcgcttg
    17521 attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca tttattccgc
    17581 cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt tctttaatac
    17641 catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca agaatcttcc
    17701 tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa ttcaagtata
    17761 taataaattt ctaattttgg gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta
    17821 attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag agggaaaagc
    17881 cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc atgcatttaa
    17941 agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct atgaaaacga
    18001 actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg
    18061 agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg acaatggcat
    18121 gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg acatctgttt
    18181 taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt ttcagtctaa
    18241 cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg atgattccgt
    18301 aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa gctgtttttt
    18361 catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt tgctgaactg
    18421 tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa atatctttta
    18481 ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta gcaactgttt
    18541 gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc gcttgagttt
    18601 gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg aattcttttg
    18661 cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct ttcgcttcgg
    18721 caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga tcagctaaat
    18781 cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct aatgcttctt
    18841 cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca gaagaggtag
    18901 aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta tctgtcggta
    18961 cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag cttatggttg
    19021 ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt cccaataaga
    19081 aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc atcacttttc
    19141 ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg gaagaaccat
    19201 aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg
    19261 gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa tgaaagtcgt
    19321 cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg cgaaaagaag
    19381 gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact ggaaaaatcc
    19441 tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat aaatctgttc
    19501 gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta cggaaataca
    19561 aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg ttcttccaat
    19621 atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt ctgttccacc
    19681 ttttgcccaa cgttttctgt gtcgatatag catcttcaac gtcacaggaa cttccatgaa
    19741 aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat cccaagcaat
    19801 actgatttct tctgtcgctc gatcttgtct gaatccgccc acatcgatca aggcttcttt
    19861 tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag ctgactgtgt
    19921 tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg caatcaattt
    19981 ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg caccagggcg
    20041 aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt
    20101 gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc
    20161 tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca gtctagctaa
    20221 tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat agttgtgata
    20281 attcaatttc gtcatcaaat actcgattgt atattcgatc acaatttctt cattatgggc
    20341 tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc attctttttg
    20401 cttatgtttg tatataaaca cataacacca gacaccaata aaccatacaa atccgcctaa
    20461 aatcggataa gaaacgagaa aaaaatacat aatagacaaa caagtatcta agaataaatt
    20521 acctgtatgg gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca
    20581 atgaatgtcg tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc
    20641 tcttcttttg ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc
    20701 aatttttcat cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc
    20761 cacctagtca atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg
    20821 attaagaaat acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct
    20881 ctttctggtc tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat
    20941 cctaaaagag ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac
    21001 ttatctttca ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct
    21061 cacgtttttt tgttacgacg taggagttta ccatacattt catttctttt cacataaagt
    21121 gttcctaaat ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat
    21181 tttgttcttt ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc
    21241 aatacgacaa tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt
    21301 tgctcatctc catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt
    21361 gctagactca aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat
    21421 tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt
    21481 agagaaacca aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg
    21541 tattcgggtt cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca
    21601 gcataggttt tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca
    21661 taccgatgga tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg
    21721 gaagaattaa agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat
    21781 aattcgatca atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga
    21841 tagcgaaatg ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg
    21901 actgcttcat caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca
    21961 taaattcttt caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt
    22021 atttttcgat attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa
    22081 tcatcagaaa tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc
    22141 tgattctgca cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc
    22201 agtgcctttt tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca
    22261 tatatttttt cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg
    22321 ataaaattcg gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt
    22381 ttgctacgca aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa
    22441 gcctcttcac cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga
    22501 ttcagtggat ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga
    22561 tgattaggtg atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc
    22621 agatacgcaa tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc
    22681 aagatatttt gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca
    22741 gtgatagcct tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct
    22801 tgaatagcta cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt
    22861 cgtctttctt taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca
    22921 atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca
    22981 tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc
    23041 agaaagtcta caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct
    23101 agctgatttt ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt
    23161 tcaatcatcg aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg
    23221 ccagccattt ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc
    23281 agttcgtttt ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac
    23341 tgtttttgtg tgttgaccat acacattctc ctcttttttt aaagtggtgg aaccgattag
    23401 tttacctctt ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat
    23461 cagttcttca ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg
    23521 gtagccagac tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct
    23581 gacgatatat tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg
    23641 gatatgtttg gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa
    23701 taaaattttt ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag
    23761 cccttctgca atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc
    23821 aaatggcggt gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt
    23881 gattgctgtt gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc
    23941 accataaatc tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc
    24001 tggataatag cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt
    24061 ttctttggct gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat
    24121 aattgctgtt ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac
    24181 ttgtctacct tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat
    24241 ttgctgtttt acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata
    24301 acgttcgtct actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag
    24361 ctggttatca ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc
    24421 atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat
    24481 cacttctact ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag
    24541 tgttggaaga atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag
    24601 gttttctgcc atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg
    24661 tttttctaat gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt
    24721 gtcggcttct gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac
    24781 caatgcatcg tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt
    24841 ttcgggaaag atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga
    24901 taatgattct ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata
    24961 ataaggcaag ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc
    25021 tgcatcttcc attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc
    25081 gacaataacg attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc
    25141 caccacgtac attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac
    25201 tcctcatccc tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt
    25261 ttatactttg taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag
    25321 gtgttctaac atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg
    25381 ttatcgattt tattgattga aatagcaaat tcattttctg cacctcattt acatttttat
    25441 aaatacctca tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct
    25501 tcattttttt cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt
    25561 ataaaaatag aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga
    25621 aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta
    25681 tggatgtcat cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga
    25741 tcgttgcaga taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag
    25801 cggctaaact agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg
    25861 aaagtcgttt tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa
    25921 aacctgataa aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt
    25981 accggttatc tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac
    26041 gcttgcgcca atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta
    26101 atcgttgtac cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat
    26161 atcaagcctt agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt
    26221 atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct
    26281 gcatcaagt
//
LOCUS       DACEMK010000036        25943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEMK010000036 DACEMK010000000
VERSION     DACEMK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.036"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW715_001257"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW715_001257"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1021871.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW715_001258"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW715_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1021872.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="IW715_001259"
     CDS             complement(3549..4358)
                     /locus_tag="IW715_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1021873.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW715_001260"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW715_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1021874.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="IW715_001261"
     CDS             complement(7968..8654)
                     /locus_tag="IW715_001261"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1021875.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDYQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="IW715_001262"
     CDS             complement(9002..10783)
                     /locus_tag="IW715_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1021876.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="IW715_001263"
     CDS             complement(10809..11723)
                     /locus_tag="IW715_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021877.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="IW715_001264"
     CDS             complement(11750..12712)
                     /locus_tag="IW715_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021878.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="IW715_001265"
     CDS             complement(12712..13656)
                     /locus_tag="IW715_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021879.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="IW715_001266"
     CDS             complement(13657..14661)
                     /locus_tag="IW715_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021880.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW715_001267"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW715_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1021881.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW715_001268"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW715_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1021882.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW715_001269"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW715_001269"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1021883.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="IW715_001270"
     CDS             complement(18496..20178)
                     /locus_tag="IW715_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1021884.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="IW715_001271"
     CDS             complement(20230..20592)
                     /locus_tag="IW715_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1021885.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="IW715_001272"
     CDS             20862..21050
                     /locus_tag="IW715_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1021886.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="IW715_001273"
     CDS             21288..23957
                     /locus_tag="IW715_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1021887.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="IW715_001274"
     CDS             complement(24048..24668)
                     /locus_tag="IW715_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1021888.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="IW715_001275"
     CDS             24780..25181
                     /locus_tag="IW715_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021889.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="IW715_001276"
     CDS             25316..25654
                     /locus_tag="IW715_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ1021890.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7027 a         5498 c         4644 g         8774 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaattca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat aatctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctggaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa aataagtagg gcaaaatata
    25921 aatgtttttg ccttacttgt ttc
//
LOCUS       DACEMK010000037        25675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEMK010000037 DACEMK010000000
VERSION     DACEMK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.037"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..514
                     /locus_tag="IW715_001277"
     CDS             170..514
                     /locus_tag="IW715_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1021891.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IW715_001278"
                     /pseudo
     CDS             515..934
                     /locus_tag="IW715_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IW715_001279"
     CDS             complement(1026..1376)
                     /locus_tag="IW715_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021892.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IW715_001280"
     CDS             complement(1342..2673)
                     /locus_tag="IW715_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1021893.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IW715_001281"
     CDS             2929..3495
                     /locus_tag="IW715_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1021894.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IW715_001282"
     tRNA            3640..3727
                     /locus_tag="IW715_001282"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IW715_001283"
     CDS             4061..5035
                     /locus_tag="IW715_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1021895.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IW715_001284"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IW715_001284"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1021896.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IW715_001285"
     CDS             complement(7450..7896)
                     /locus_tag="IW715_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1021897.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IW715_001286"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IW715_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1021898.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IW715_001287"
     CDS             8361..8879
                     /locus_tag="IW715_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1021899.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IW715_001288"
     CDS             complement(8956..9912)
                     /locus_tag="IW715_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021900.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IW715_001289"
     CDS             10078..10884
                     /locus_tag="IW715_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021901.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IW715_001290"
     CDS             10881..11870
                     /locus_tag="IW715_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1021902.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IW715_001291"
     CDS             11867..12871
                     /locus_tag="IW715_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1021903.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IW715_001292"
     CDS             13003..13545
                     /locus_tag="IW715_001292"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1021904.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IW715_001293"
     CDS             13565..14263
                     /locus_tag="IW715_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1021905.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IW715_001294"
     CDS             14284..14496
                     /locus_tag="IW715_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1021906.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IW715_001295"
     CDS             14496..15458
                     /locus_tag="IW715_001295"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1021907.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IW715_001296"
     CDS             15476..15871
                     /locus_tag="IW715_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1021908.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IW715_001297"
     CDS             16034..16399
                     /locus_tag="IW715_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1021909.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IW715_001298"
     CDS             16396..18207
                     /locus_tag="IW715_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1021910.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IW715_001299"
     CDS             complement(18269..18436)
                     /locus_tag="IW715_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1021911.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IW715_001300"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IW715_001300"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1021912.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IW715_001301"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IW715_001301"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1021913.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IW715_001302"
     CDS             21193..22173
                     /locus_tag="IW715_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1021914.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IW715_001303"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IW715_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1021915.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IW715_001304"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IW715_001304"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1021916.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IW715_001305"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IW715_001305"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1021917.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IW715_001306"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IW715_001306"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1021918.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4397 c         5376 g         7575 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaata aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt ttttctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggta aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctgggtgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tattt
//
LOCUS       DACEMK010000038        25598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEMK010000038 DACEMK010000000
VERSION     DACEMK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.038"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW715_001307"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IW715_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1021919.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IW715_001308"
     CDS             808..1242
                     /locus_tag="IW715_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1021920.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IW715_001309"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IW715_001309"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1021921.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IW715_001310"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IW715_001310"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1021922.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IW715_001311"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IW715_001311"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1021923.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IW715_001312"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IW715_001312"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1021924.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IW715_001313"
     CDS             9513..10439
                     /locus_tag="IW715_001313"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1021925.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IW715_001314"
     CDS             10621..11514
                     /locus_tag="IW715_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1021926.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IW715_001315"
     CDS             11648..11773
                     /locus_tag="IW715_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1021927.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IW715_001316"
     CDS             complement(11907..12539)
                     /locus_tag="IW715_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021928.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IW715_001317"
     CDS             complement(12687..13229)
                     /locus_tag="IW715_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1021929.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IW715_001318"
     CDS             13364..13753
                     /locus_tag="IW715_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021930.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IW715_001319"
     CDS             13757..14521
                     /locus_tag="IW715_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1021931.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IW715_001320"
     CDS             14650..15597
                     /locus_tag="IW715_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1021932.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IW715_001321"
     CDS             15727..16467
                     /locus_tag="IW715_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021933.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="IW715_001322"
     CDS             complement(16758..17426)
                     /locus_tag="IW715_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1021934.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="IW715_001323"
     CDS             17560..17847
                     /locus_tag="IW715_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021935.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="IW715_001324"
     CDS             18263..19801
                     /locus_tag="IW715_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1021936.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="IW715_001325"
     CDS             19798..20763
                     /locus_tag="IW715_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021937.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="IW715_001326"
     CDS             20750..22072
                     /locus_tag="IW715_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1021938.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="IW715_001327"
     CDS             22313..23056
                     /locus_tag="IW715_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1021939.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="IW715_001328"
     CDS             23071..24063
                     /locus_tag="IW715_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1021940.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="IW715_001329"
     CDS             24075..24812
                     /locus_tag="IW715_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1021941.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="IW715_001330"
     CDS             24802..25524
                     /locus_tag="IW715_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1021942.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         8865 a         4337 c         5324 g         7072 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaat
//
LOCUS       DACEMK010000039        25481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEMK010000039 DACEMK010000000
VERSION     DACEMK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.039"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(36..722)
                     /gene="gpmA"
                     /locus_tag="IW715_001331"
     CDS             complement(36..722)
                     /gene="gpmA"
                     /locus_tag="IW715_001331"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1021943.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(894..1475)
                     /locus_tag="IW715_001332"
     CDS             complement(894..1475)
                     /locus_tag="IW715_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1021944.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1608..2291)
                     /gene="rpiA"
                     /locus_tag="IW715_001333"
     CDS             complement(1608..2291)
                     /gene="rpiA"
                     /locus_tag="IW715_001333"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1021945.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2610..3023
                     /gene="rpsL"
                     /locus_tag="IW715_001334"
     CDS             2610..3023
                     /gene="rpsL"
                     /locus_tag="IW715_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1021946.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3107..3577
                     /gene="rpsG"
                     /locus_tag="IW715_001335"
     CDS             3107..3577
                     /gene="rpsG"
                     /locus_tag="IW715_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1021947.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3671..5758
                     /gene="fusA"
                     /locus_tag="IW715_001336"
     CDS             3671..5758
                     /gene="fusA"
                     /locus_tag="IW715_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1021948.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5930..7117
                     /gene="tuf"
                     /locus_tag="IW715_001337"
     CDS             5930..7117
                     /gene="tuf"
                     /locus_tag="IW715_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1021949.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7524..7820
                     /gene="rpsJ"
                     /locus_tag="IW715_001338"
     CDS             7524..7820
                     /gene="rpsJ"
                     /locus_tag="IW715_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1021950.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIQYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL"
     gene            7851..8480
                     /gene="rplC"
                     /locus_tag="IW715_001339"
     CDS             7851..8480
                     /gene="rplC"
                     /locus_tag="IW715_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1021951.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8508..9131
                     /gene="rplD"
                     /locus_tag="IW715_001340"
     CDS             8508..9131
                     /gene="rplD"
                     /locus_tag="IW715_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1021952.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9131..9421
                     /gene="rplW"
                     /locus_tag="IW715_001341"
     CDS             9131..9421
                     /gene="rplW"
                     /locus_tag="IW715_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1021953.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9460..10293
                     /gene="rplB"
                     /locus_tag="IW715_001342"
     CDS             9460..10293
                     /gene="rplB"
                     /locus_tag="IW715_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1021954.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10336..10614
                     /gene="rpsS"
                     /locus_tag="IW715_001343"
     CDS             10336..10614
                     /gene="rpsS"
                     /locus_tag="IW715_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1021955.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10637..10984
                     /gene="rplV"
                     /locus_tag="IW715_001344"
     CDS             10637..10984
                     /gene="rplV"
                     /locus_tag="IW715_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1021956.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10998..11654
                     /gene="rpsC"
                     /locus_tag="IW715_001345"
     CDS             10998..11654
                     /gene="rpsC"
                     /locus_tag="IW715_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1021957.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11657..12091
                     /gene="rplP"
                     /locus_tag="IW715_001346"
     CDS             11657..12091
                     /gene="rplP"
                     /locus_tag="IW715_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1021958.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12081..12269
                     /gene="rpmC"
                     /locus_tag="IW715_001347"
     CDS             12081..12269
                     /gene="rpmC"
                     /locus_tag="IW715_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1021959.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12294..12560
                     /gene="rpsQ"
                     /locus_tag="IW715_001348"
     CDS             12294..12560
                     /gene="rpsQ"
                     /locus_tag="IW715_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1021960.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12618..12986
                     /gene="rplN"
                     /locus_tag="IW715_001349"
     CDS             12618..12986
                     /gene="rplN"
                     /locus_tag="IW715_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1021961.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13023..13331
                     /gene="rplX"
                     /locus_tag="IW715_001350"
     CDS             13023..13331
                     /gene="rplX"
                     /locus_tag="IW715_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1021962.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13359..13898
                     /gene="rplE"
                     /locus_tag="IW715_001351"
     CDS             13359..13898
                     /gene="rplE"
                     /locus_tag="IW715_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1021963.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13917..14102
                     /locus_tag="IW715_001352"
     CDS             13917..14102
                     /locus_tag="IW715_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1021964.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14138..14536
                     /gene="rpsH"
                     /locus_tag="IW715_001353"
     CDS             14138..14536
                     /gene="rpsH"
                     /locus_tag="IW715_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1021965.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14568..15104
                     /gene="rplF"
                     /locus_tag="IW715_001354"
     CDS             14568..15104
                     /gene="rplF"
                     /locus_tag="IW715_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1021966.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15265..15621
                     /gene="rplR"
                     /locus_tag="IW715_001355"
     CDS             15265..15621
                     /gene="rplR"
                     /locus_tag="IW715_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1021967.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15642..16142
                     /gene="rpsE"
                     /locus_tag="IW715_001356"
     CDS             15642..16142
                     /gene="rpsE"
                     /locus_tag="IW715_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1021968.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16157..16336
                     /gene="rpmD"
                     /locus_tag="IW715_001357"
     CDS             16157..16336
                     /gene="rpmD"
                     /locus_tag="IW715_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1021969.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16380..16820
                     /gene="rplO"
                     /locus_tag="IW715_001358"
     CDS             16380..16820
                     /gene="rplO"
                     /locus_tag="IW715_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1021970.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16820..18115
                     /gene="secY"
                     /locus_tag="IW715_001359"
     CDS             16820..18115
                     /gene="secY"
                     /locus_tag="IW715_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1021971.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18177..18824
                     /locus_tag="IW715_001360"
     CDS             18177..18824
                     /locus_tag="IW715_001360"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1021972.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19015..19233
                     /gene="infA"
                     /locus_tag="IW715_001361"
     CDS             19015..19233
                     /gene="infA"
                     /locus_tag="IW715_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1021973.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19266..19382
                     /gene="rpmJ"
                     /locus_tag="IW715_001362"
     CDS             19266..19382
                     /gene="rpmJ"
                     /locus_tag="IW715_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1021974.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19400..19765
                     /gene="rpsM"
                     /locus_tag="IW715_001363"
     CDS             19400..19765
                     /gene="rpsM"
                     /locus_tag="IW715_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1021975.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19793..20182
                     /gene="rpsK"
                     /locus_tag="IW715_001364"
     CDS             19793..20182
                     /gene="rpsK"
                     /locus_tag="IW715_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1021976.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20262..21200
                     /locus_tag="IW715_001365"
     CDS             20262..21200
                     /locus_tag="IW715_001365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1021977.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21228..21608
                     /gene="rplQ"
                     /locus_tag="IW715_001366"
     CDS             21228..21608
                     /gene="rplQ"
                     /locus_tag="IW715_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1021978.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21880..22719
                     /locus_tag="IW715_001367"
     CDS             21880..22719
                     /locus_tag="IW715_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1021979.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22695..23564
                     /locus_tag="IW715_001368"
     CDS             22695..23564
                     /locus_tag="IW715_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1021980.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23554..24351
                     /locus_tag="IW715_001369"
     CDS             23554..24351
                     /locus_tag="IW715_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1021981.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24554..25300
                     /gene="truA"
                     /locus_tag="IW715_001370"
     CDS             24554..25300
                     /gene="truA"
                     /locus_tag="IW715_001370"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1021982.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8281 a         4701 c         5484 g         7015 t
ORIGIN      
        1 agttggcagg agaaaaccct tttttatagt gttttttata ggtagtattt tttagcaact
       61 gttaagtcat catttaattc ataaacaagt ggttgacctg ttggaatttc aagatccatg
      121 atgtcttcgt cagagatacc ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt
      181 gcagctacta aaacagtttt gttgtctagt aatgctggag caatttcatc ttgccagaat
      241 ggcaaagcgc gttctaatgt cactttcaag ttttctccac caggaatatc gcgtttgtct
      301 aacattgcgt aacgacggtc atttgctgca gaaccttcgt cagtttcttc cattaatgga
      361 ggaagagtat cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta tttttcagct
      421 gtttcttttt tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa gcgccatgat
      481 ttgatttgtg gtacccaaag ttgatctgaa tattctaaga tcaagttaca tgttttgatt
      541 gcacgagtta aaacagaagt gtaagcaaca tcaaattcga ttccagcttc tttgattttg
      601 cgtccgcctt cttttgcttc ttcgattcct tctggtgcta gatttacatc agcccaacca
      661 gtaaattggt tcaatgcatt ccattcacta agaccgtgac gagaaaaaac taattttggc
      721 atgtttagct ctccttttaa gataggttta tatttctgaa attttcagtt ctgttttatt
      781 gtacaatttt ttggaagaga tttccatcga aaaattagat aagattctat gagaaaacaa
      841 gtagagaata taaaaaaacg aacagtgccc acgcaactgt tcgtttttag gtattagatg
      901 cataattgct cgatttcttt ttcaggtaaa ttgatatatt tataacatgt tccaactgaa
      961 acaccgcatt ttgaagaaat aaattggatc gtttcttttt tttcataata gagacgtcgg
     1021 atttttttga tcgtttttgc atcgattttt ggtcttccgc cgatttttcc ttttttacga
     1081 gcttcatcta atccaactaa tgtacgttct ttgatcaatg cgcactccat gtttgctagg
     1141 ttttccatta attggaaata gatttttccc atttcgcgcg tatcgattcc ttcatcaaga
     1201 ctaatcaagt gcagcccgcg atctttgaat aattgcgtga gttctgttaa ttgccgcgta
     1261 gatttgccta atcgatgaag ttggcaaatg actagtgcat cgccttgttg catgtcagcg
     1321 atcactgact ccaaatgaac agcagtagaa tcattttgtt ccacttcata tgtctcatgg
     1381 aaaatttctt gacaaccaaa gtcagataat tgatcaacct gagttgttaa atcaacgtca
     1441 tttatggttg tgcgtgcata accaataatt ttcatggtga ttcctttcta gcagtgattt
     1501 tctaactgga tataacagta aaaatgtagc ataaagtacg tcaaaaagat agttttttcg
     1561 caaattttcc gctgtctttt tttattggat gatcaagcag tttttcttta tttacgacgg
     1621 attgcgtcaa gaatctcaac gcctttttct gagccgacaa ctactttaga aaccatgttt
     1681 aggaaaagcc catgttctac taccccaaca agttcatcta aataggtagc taagctttct
     1741 ggctgttcga tgacttctag atgcagatca atgatgtaat gaccaccatc tgtggttaag
     1801 acttctccgt tttcgtctgt acgcaagacg ggttggaatc ttttttcttc aaaaatatga
     1861 agtagctgtt gggagccata agggatcact tcaactggga gtgggaactt cccaagttta
     1921 tcgactaatt tagaagaatc aacgatccaa atcgtttctt ttgagtacgt agcgactatt
     1981 ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc actgatttca
     2041 tcagcaccgt ctattgtcaa atcgactacg gggacttcat caatactctt taacggtatt
     2101 cctaattttt ccgcttgttc ttttgtcgca tttgaagtag taactcctgt gatacgtaaa
     2161 ccttcttcac gcatacggcg accaatctct tctaccatat aataggcggt tgaaccggtc
     2221 ccgagaccta cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc agctaattgt
     2281 tttaaattca tctttgcgtc cacctttttt tagtttgagc atcgtgcttt cctcactatt
     2341 ttagcgtgat ttacggtaaa aagataccat tcttttttat tccaataaag ctttttcaaa
     2401 aaaaattgca tctcttgttg acatgttttt tcattcgtga tattctaaca aaggtgcttt
     2461 atctacaggt ttctgtgaag aaacgtgctg actgtttatc aaagcatatt tgataaggat
     2521 gcagacattg catttatttt ttatcacaaa aaaagaacca cctggatgtg tggaactaga
     2581 cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat taaccaatta gtacgtaaac
     2641 ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt gaacaaagga tataacagct
     2701 ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg
     2761 gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt
     2821 caaacttaat tgaagttact gcttatatcc cagggattgg tcataactta caagaacaca
     2881 gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc aggggtacgt taccatatcg
     2941 ttcgcggtgc tttagatact gctggtgtta acgaccgtaa acaaagccgt tctaaatacg
     3001 gaactaaaaa acctaaagct taattttaat tattaaacaa atgaaacttg cttctactat
     3061 atagaagtaa ttactatgaa caatatttcg gaaggaggag ttacggatgc cacgtaaagg
     3121 tcctgttgca aaacgtgatg ttttaccaga tcctatttat aactcaaaat tagtaactcg
     3181 cttgatcaac cgtgtaatgg ttgacggaaa acgcgggatt gcagctaaca ttatctataa
     3241 tgcctttgac ttgatcaaag aatctacagg taacgatcca ttagaagtgt ttgaacaagc
     3301 aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta
     3361 ccaagtacca gttgaagttc gccctgaacg ccgcgttact ctaggtcttc gttgggtagt
     3421 aaactacgct cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa aagaaatcat
     3481 ggatgctgca aacaacacag gtgcttctgt taaaaaacgt gaagacactc ataaaatggc
     3541 tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt tggtcggctt
     3601 ttataagtcg acctcagata gtgtccttat ttttagcaac tagatagtaa agagaggaga
     3661 aacaaggaga atgaccagag aattttcttt acaaaacact cgtaatatcg gtatcatggc
     3721 ccacatcgat gctggtaaaa cgactgctac tgaacgtatc ttgtactata ctggtaaaat
     3781 ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg gactggatgg aacaagaaca
     3841 agaacgtggt attacgatca cttctgctgc tactacagca caatggaagg gtcaccgaat
     3901 caacatcatc gacacaccag gacacgtaga cttcacagtt gaagttgaac gttcattgcg
     3961 tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac ctcaaactga
     4021 aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg caaacaaaat
     4081 ggacaagatc ggtgctgact tcttatattc agtatcaact ttacatgacc gtttgcaagc
     4141 aaacgctcac ccaatccaat taccaattgg tgcggaagac gacttcacag gaattatcga
     4201 ccttgtaaca atgaaggctg aaatgtatac aaacgatctt ggaacagaga tcgaagaaac
     4261 agaaattcct gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc
     4321 tgtagctgaa actgatgaag aacttacttt gaaatactta gaaggtgaag aaatcactga
     4381 agctgagtta aaagaaggta tccgccgcgc aacagttaac gtagaattct acccagtact
     4441 atgtggttca gcatttaaga acaaaggggt tcaattacta ttggacgctg ttcttgatta
     4501 cttgccatca ccgttagata tcccagctat caaagggatt gaccctaaaa ctgatgaaga
     4561 agtagaacgt cctgcagacg acagcgctcc tttctcagct ttagcattta aagttatgac
     4621 tgacccattc gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt tacaatcagg
     4681 ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta tcctacagat
     4741 gcatgccaac tctcgttcag aaatctctga agtttacgct ggagatatcg ctgctgctgt
     4801 tggtttgaaa gacacaacta ctggggacac actatgtgat gaaaagagtc tagttattct
     4861 tgaatccatg gaattcccag aaccagttat ccaagtcgct attgaaccta aatcaaaagc
     4921 tgaccaagat aaaatgggtg tggctttaca aaaactatct gaagaagatc ctactttccg
     4981 tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg aacttcactt
     5041 agatatcatc gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc
     5101 tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta agtttgtacg
     5161 tcagtctggt ggtaaaggtc aatacggtca cgtatggatt gaatttacac caaatgaaga
     5221 aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat
     5281 tccagctgtt gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc
     5341 attagtagat atcaaagcta aactttacga tggttcatac catgatgtcg attcaaatga
     5401 aactgccttc cgtgttgctg cttctatggc attacgtgca gcagctaaga aagctaatcc
     5461 agtaatcctt gaaccaatca tggctgtaga agttgttatc cctgaagatt atttaggtga
     5521 tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg
     5581 cggccaacaa gttgttcgtg cgatggttcc acttgctgaa atgtttggtt atgctacaac
     5641 tcttcgttct gctactcaag gtcgtggtac atttacaatg acatttgacc actacgaaga
     5701 tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac ggcggaaaag ctgaataatt
     5761 tcttaaaacc ggatagtcct gcaaagagat tatccaaaga gactatattt ttaatatagt
     5821 ttcgtgtaaa ataattatcg atgatatggg catgtggtgt accttgtgta catcatatac
     5881 ctatcagaca aataaaaaat atattgtatt tttaggagga cattttaaaa tggcaaaaga
     5941 aaaatttgac cgttctaaac cacacgttaa cattggtact atcggacacg ttgaccatgg
     6001 taaaactaca ttaacagctg caatcacaac tgtactatct aagaaaaacg gcggccaagc
     6061 tatggcttac gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa tcacaatctc
     6121 aacagctcac gttgagtatg aaacagatac acgccactac gctcacgttg actgcccagg
     6181 acacgcggat tacgttaaaa acatgatcac tggtgctgct caaatggacg gagctatctt
     6241 ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg
     6301 tcaagttggt gttccttaca tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga
     6361 agaattacta gaattagttg aaatggaagt tcgtgaccta ttaacagaat acgaattccc
     6421 tggtgacgat gttcctgtag ttgctggatc agctttgaaa gctctagaag gcgacgcttc
     6481 atacgaagaa aaaattcttg aattaatggc tgcagttgac gaatacatcc caactccaga
     6541 acgtgacaac gacaaaccat tcatgatgcc agttgaagac gtgttctcaa ttactggacg
     6601 tggtactgtt gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg acgaagttga
     6661 agttgttggt attgctgaag aaacttcaaa aacaacagtt actggtgttg aaatgttccg
     6721 taaattgtta gactacgctg aagctggaga caacattggt gctttactac gtggtgttgc
     6781 acgtgaagac atccaacgtg gacaagtttt agctaaacca ggtacaatca cacctcatac
     6841 aaaattctct gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc atactccatt
     6901 cttcactaac taccgtccac aattctactt ccgtacaact gacgtaacag gtgttgttga
     6961 attaccagaa ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg aagttgaatt
     7021 aatccaccca atcgctatcg aaaacggtac taaattctca atccgtgaag gcggacgtac
     7081 agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc acttaagttt taaagtaaag
     7141 caacatttat tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg ttccgagctt
     7201 ttttatttat tgcaggatca ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa
     7261 ataaaagagt cagtattttc taaatgaaac tttatgaaaa caaaatgttt tttacaagaa
     7321 aaagccttgc tttgtgagtt tatatatatt ataatgacaa aggtgcgttt actttagtga
     7381 attgcacaga cgtaatcaga atgatttcgt cggctaagaa acgagaggtt gcgacacgcc
     7441 cggacgcttt gccacgaacg agcgtgacgg aaattttcgt ggagctatgt ctacttttca
     7501 aaataggcga aggagggaac aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt
     7561 atgaacaccg tattttagat caatcagcgg ataaaatcgt ggaaactgca aaaagaactg
     7621 gagctagcgt gtctggtcca attccattac caactgaacg cagtctttac acaatcatcc
     7681 aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt atcgacattg
     7741 tgaacccaac accaaaaact gttgatgctt tgatgaagct agacttacca tctggtgtaa
     7801 acatcgaaat caaattataa aactaaaaca aaaataatgg aggtgtactc atgaccaaag
     7861 gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt gaattaattc
     7921 cagtaactgt tgtagaagct actccaaacg tagtactaca agtgaaaaca atggaaacgg
     7981 atggctacga agctattcaa gttggttacc aagatatgcg tgaagtttta tcaaacaaac
     8041 ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt cgtgaattca
     8101 aaaatgttga gctaggagaa tatgaagtag gaaaagaaat tacagtagat gttttccaag
     8161 caggagacat tattgatgta acaggtacta cgaaaggtaa aggattccaa ggggttatca
     8221 aacgccacgg acaaagccgc ggaccaatgg ctcacggttc tcgttaccat cgtcgtcctg
     8281 ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa caaacgtctt gctggccgta
     8341 tgggtggcaa ccgcgtaact atccaaaact tggaagttgt acgtgtcgat gcagaaagaa
     8401 atgttatctt gatcaaaggt aatgttcctg gagctaaaaa atctttaatt actatcaaat
     8461 cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg ccgaatgtag
     8521 cattattcaa acaagatgga agccaaaacg gtgaaatcac acttaacgaa gaaatcttcg
     8581 gaattgaacc taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt gcgtcattaa
     8641 gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga cgtaaaccat
     8701 ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat ccgctcacca caatggcgtg
     8761 gaggtggcgt agtctttgga ccaacaccac gttcttacag ctacaaactt cctaaaaaag
     8821 ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac aacttggtag
     8881 caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa gttcttgcta
     8941 acttatctat cgactctaaa gtattggttg ttctagaaaa aggtaacgac tttgctgctt
     9001 tatcagcacg taacttacca aacgtttctg tagtaacttc tgataatgtg agtgtgctag
     9061 acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa attgaggagg
     9121 tgcttgcata atgaacttac tagacgtaat caaacgccca gtgatcactg aaaaatcaat
     9181 gcttgccatg gatgacaaga aatacacttt tgaagtggac actcgcgcaa acaaaacttt
     9241 agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga acatcgtgaa
     9301 cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga tatacaaaaa aacgtcgcaa
     9361 agcgatcgtt acattgactg aagattcaaa agaaatccaa ttattcgaag ctgctgaata
     9421 agcactaata gataactcat taaattagga gggaaaaacg tggcgattaa aaagtacaaa
     9481 cctacctcaa atggtcgtcg taacatgaca ggttctgatt ttgctgaaat cacaacatca
     9541 acacctgaaa aatcattgtt acaaccattg aaaaaccatg ctggacgtaa caacaacggc
     9601 cgcatcactg tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt gatcgacttc
     9661 aaacgtaaca aagataacgt tgttgcaact gttcaaacga tcgaatatga tccaaaccgt
     9721 tcagctaaca tcgcgttagt acactatgaa gatggagtga aagcttacat cttagcacct
     9781 aaaggactag aagtgggtat gaaacttgtt tcaggtccag aagcagatat caaagtcgga
     9841 aatgctcttc cattagaaaa cattccagtt ggtactgtta tccacaacat tgaaatgaaa
     9901 cctggtaaag gtggacaatt gatccgttct gctggtacaa gtgctcaagt acttggtaaa
     9961 gaaggcaaat acgtattgat ccgcttgaac tctggtgaag ttcgtatgat cttagctgca
    10021 tgccgtgcta caatcggttc tgtaggtaac gaacaacacg aattaatcaa catcggtaaa
    10081 gctggacgct ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt aatgaaccct
    10141 aatgatcacc cacacggtgg tggtgaaggt aaagctccta tcggacgtaa agcaccagtt
    10201 tctccttggg gtcaaccagc tcttggttac aaaactcgta acaaaaaagc taaatcagac
    10261 aaacttattg ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt cgcacatttt
    10321 gagaggaggt tcaccatggg tcgtagttta aagaaaggac cttttgtcga tgagcattta
    10381 atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa aacttggtca
    10441 cgccgttcaa cagttttccc aagttttgtt ggatatacca tcgcagttta tgatggacgt
    10501 aaacatgtac cagtttacat ccaagaagac atggtaggac acaaattagg tgaatttgca
    10561 cctacaagaa cttatcgtgg acatgctgct gacgataaga aaacgaaacg ctaatttgag
    10621 gggaggataa atccaaatgg cagaacaaat cacatcagct aaagcaactg caaaaacagt
    10681 tcgcacttca cctcgaaaag cccgtttagt aattgacctt atcagaggta aaagtgtcgc
    10741 agatgcaatt tcaatcttga aattcacacc aaacaaatct gctggaatca ttgaaaaagt
    10801 attgatgtca gcagttgcga atgcagaaaa taactttgac ttagatgttg aaaacttggt
    10861 agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc ctcgtgcaaa
    10921 aggatcagct tctccaatca acaaacgtac aagtcatatc acggtagtcg tatcagaaaa
    10981 ataaggaggg ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt gtaggcatca
    11041 tccgcgactg ggatgctaaa tggtatgctg aaaaagagta tgctgagttc ttacacgaag
    11101 atttaagaat ccgtaaattt atttcaacta aacttgctga tgctgctgta tcaacagttg
    11161 aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa ccaggtatgg
    11221 ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa agaattaaac aaattaactg
    11281 gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc agatctagat gcaaaattag
    11341 taggtgaagg aatcgcacgt caattagaaa accgtgttgc tttccgtcgc gctcaaaaac
    11401 aagccatcca acgctcaatg cgtgctggcg ctcaagggat caaaactcaa gtatctggtc
    11461 gtctaaacgg agcagatatc gctcgttcag aaggatactc agaaggaact gttcctcttc
    11521 atactttacg tgcggacatt gattacgcat gggaagaagc agatacaaca tacggaaaac
    11581 taggagttaa agtgtggatc tatcgtggag aaattcttcc aactaaaaaa aacactgaga
    11641 aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg aattccgcgg
    11701 aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca ttcggtgaat acggtttgca
    11761 agctgttgat tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta tcgcaatgac
    11821 tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca aatcttatac
    11881 tgccaaagca attggggtac gtatgggttc tggtaaaggg gcacctgaag gatgggttgc
    11941 accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag aagtagctcg
    12001 tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa actaagatcg taaaacgtga
    12061 ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag agaattaacc actgccgaaa
    12121 tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa tcttagattc caattagcaa
    12181 caggtcaatt agaaaacact gcacgtatta aagaagtacg taaatcgatt gcacgcatca
    12241 aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga ggccattaag cgtatgactg
    12301 aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa atggataaaa
    12361 caattaccgt tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa cgcatgaact
    12421 attctaagaa atacaaagtg catgatgaaa acaacacagc aaaagttggc gacatcgtga
    12481 gaattatgga aactcgtcca ttatcagcta caaaacgttt ccgtttgttg gaagtagtcg
    12541 aagaagcagt tattatctaa taacacgaga ttttcctata tagattgaat gcaaaatctg
    12601 aaaggaggat acacaacgtg atccaagcag aaagtcgttt aaaagtcgct gataactcag
    12661 gcgcacgtga aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa actgctaaca
    12721 tcggtgatgt gatcgttgct acggttaaac aagcaacgcc aggtggggtt gtcaaaaaag
    12781 gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt acagacggtt
    12841 catatattaa atttgatgaa aatgctgcag tgattattcg tgatgataaa agccctcgtg
    12901 gaacacgtat ctttgggcca gttgcacgtg aactacgtga aaacaacttc atgaagatcg
    12961 tttcactagc accagaagtt ttataatctt gagacactta tcaaaggagg tgcgaaacag
    13021 ttatgtttgt taaaaaaggc gataaagtta aagttatcac tggaaaagac aaaaacaaag
    13081 aaggcgttgt actagcagcg ttcccgaaaa aagataaagt tattgtcgaa ggtgtaaatg
    13141 tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca aggtggaatt gttgagatgg
    13201 aagcgccaat tcatgtttct aacgtgatgg tggttgactc tactggtgta gctggccgag
    13261 ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc ggtgaagttt
    13321 tagataaata aaagtttaag gaaggagggt cttactgaat gaaccgccta aaagaaaaat
    13381 atcttaaaga agtaactcca tcattgatgg aaaaatttaa ctatagctct gttatgcaaa
    13441 cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta tcaaacgcta
    13501 aaaacttaga taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa ccaatgatta
    13561 caaaagctaa aaaatcaatc gctggattcc gtttacgtga aggaatgcca attggtgcaa
    13621 aagttacatt gcgcggagaa agaatgtacg aattcttaga caaattggtt tctgtttcac
    13681 taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt cgtggaaact
    13741 atactttagg tattaaagaa caattgatct tcccagaagt agattatgat ttagtagata
    13801 aagtacgtgg tatggacatc gttattgtaa caacagcaaa ctctgatgaa gaatctcgtg
    13861 agttgttgac acaattaggc atgccattcc aaaaataatt aaaggaggcg aactacgtgg
    13921 ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa attttcaaca caagcttata
    13981 ctcgttgcga acgttgcgga cgtccacatt cagtttatcg taagttccat ctttgccgta
    14041 tttgcttccg cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa gctagctggt
    14101 aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg gtcatgacag atccaattgc
    14161 agattttcta actcgtatcc gtaacgcaaa catggttaaa catgagtctt tagaagtacc
    14221 tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt tcgttcgtga
    14281 tgttgaatac atcgaagatg acaaacaagg cgtgatccgt gttttcctaa aatttggtaa
    14341 aaacggcgaa cgtgttatca caaacttaaa acgtatctct aaaccaggtt tgcgtgctta
    14401 tgtcaaagct gacgaagtac caaaagtatt gaacggttta ggtatcgcaa tcatttcgac
    14461 ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg gcgaagttat
    14521 cgcttacgta tggtaatcaa gtagaatcta caaggaggtg tctttaagtg agccgtatcg
    14581 gaaataaagt cgttgttatc cctgaaggag taacagttac tcaagacgga aacaacgtta
    14641 cagttaaagg accaaaaggt gaattgactc gtactttttc agcagatatc aaaatgaata
    14701 tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag taaagaaatg aaaacaattc
    14761 atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa ggtttccaaa
    14821 aagcgttaga actaatcggg gttgggtacc gtgcacaatt acaaggatca aaacttgtat
    14881 tgaacgttgg ttactcacat ccagtagaaa tcacaccacc agctggagta actgttgaag
    14941 taccagctaa cacacaagta attgttaaag gtgcgaacaa agaagaagtt ggcgaattag
    15001 ctgcaaacat tcgtggaact cgtcctccag aaccttataa aggtaaaggt attcgttatg
    15061 ttggcgaata tgtacgccgt aaagaaggta aaactggtaa ataatcgttt agaaaaattg
    15121 catagaagcc agcagcagga aaaaggcatt tggctgaagt ttaccttcaa aaaaatacat
    15181 ttttacttgt gccgcagctt ctatttttcg aaacgtattt actgcttgaa atagtagtac
    15241 aataaaaata aagaggtgac aattgtgatt acaaaaccag ataagaacaa aacacgtcaa
    15301 aagagacatc gtcgtgtacg taacaatatc tctggtactg ctgagcgccc acgcttgaac
    15361 gtttttcgtt ctaacaaaaa catctacgct caaattattg atgacgtagc gggtgtaacg
    15421 ctagcaagtg cctctacctt ggataaagaa atttcaggtg gaacaaaaac agaagcagca
    15481 caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa agtagtagtc
    15541 tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag ctttagctga agctgctcgc
    15601 gaaaatggac tagaatttta gaagaaggag gaataccatt catggtttat atcgatccaa
    15661 aacatttgga attagaagac cgcgttgttg caattaaccg tgtaacaaaa gttgttaaag
    15721 gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac ggacacgtag
    15781 ggtttggtac aggtaaagct caagaagttc ctgaagcaat ccgtaaagca attgaagatg
    15841 cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac aatcccacac gaagtaatcg
    15901 gtgcttttag tggtggtaga atcctaatga aacctgctgt agaaggttct ggggttgctg
    15961 ctggtggacc agttcgtgcc gtattagaat tagctggggt agcagatatc acttctaaat
    16021 ctttaggctc aaatacacca atcaacgttg ttcgcgcaac agttgaaggt ttgaaacaat
    16081 tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc agttgaagaa ttaatcggat
    16141 aaggaggaca acaataatgg ctgaattaaa agttacttta aaacgcagta ttatcggacg
    16201 tcctcaaaac caacgcgata cagttaaagc gttaggtctt acaaagatta acagttcagt
    16261 ggttaagcct tctaatgaag caattaaagg catgatcaat actgtttctc acctagtgga
    16321 cgttgaagaa atttaattta gaaaaatcat aactagaaca ttaaggaggt gccaaaccta
    16381 tgaaacttca tgaattaaaa cctgctgaag gttcacgcca agtacgtaac cgtgttggtc
    16441 gtggtacttc atctggtaac ggaaaaactg ccggacgcgg acaaaaagga caaaaagctc
    16501 gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca aacaccattg ttccgtcgtt
    16561 taccaaaacg aggattcaca aacgtgaacc gtaaagacta tgcagtcatt aatcttgatg
    16621 tattgaaccg ctttgaagac ggagctgaag taactcctgt tgctttagtt gaagctggaa
    16681 tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa tggggaacta aacaagaaat
    16741 taactgtgaa agcagcaaaa ttctctaaag cagctcaaga agcaattgaa gcggctggtg
    16801 gatcaatcga ggtgatctaa tgtttagact attaaaagat gcttttaaag taaaagacat
    16861 tagatcgaaa atcctattta ctgtatttgt gttgtttgta tttcgcctag gtgcacatat
    16921 tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc catttttcaa
    16981 catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt tccattttct caatgggggt
    17041 ttcaccatac attactgctt ctatcgtcat tcagcttctt caaatggaca tcgtgcctcg
    17101 atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc aagcgactcg
    17161 atatctgaca ttagtattgg catttatcca atctgtcggt atcactgctg gattcaatca
    17221 atacgctcaa ttaggtttcg tgaataatcc taacgtagca acttacgtaa cgatcggttt
    17281 gattctaaca gccggtacga tgtttgttac ttggctagga gaacagatca cagaaaaagg
    17341 tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc atttctagat tgcctgaaag
    17401 tatcagagaa atttatgaag attactttat aaatattgat ccttcagaca tatggaaatc
    17461 tgctattttt gtggccattt tactagtagc ggtcttgttg atcgtgacat tcgtaacatt
    17521 tttccaacaa gctgaacgca agatcccgat ccaatatacg aaacgcgtag ctggcgcgcc
    17581 tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga gttattccag ttatctttgc
    17641 tagttcgttt attgctacgc caaatgctgt tttacaagca ttgagcggta gattcggttc
    17701 tgaaaactgg tacaaagtga tgatggagat ctttaactat aatacagcac ctggtgctac
    17761 gatttatacg attctgatcg ttgccttcac tttcttctac gcctttgttc aagtaaaccc
    17821 tgagaaatta gcggaaaact tgcaaaagca aggaagttat ataccaagcg tacgccctgg
    17881 taaggggact gaagagtatg tctcaagatt actgatgcgt ttaagtacag ttggtgcagt
    17941 cttccttggt ttagtggcat tgcttccaat cattgctcaa atggtttggg atcttccaca
    18001 atcaatcgga ttaggcggta caagcttact gatcgttatc ggtgtagctt tagaaactgc
    18061 gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt ggattcattg agtaagaaag
    18121 cgtgttgagg tttcccctca acacacgttc tcacacattt attaggaggg aaaacaatga
    18181 acctcatttt aatggggctg ccaggcgcag gtaaaggaac acaagctgaa aaaatcattg
    18241 atgcttatgg tattccgcac atctcaacag gagatatgtt ccgcgcagcg atgcaaaacg
    18301 aaacagctct tggcctggaa gcaaagtctt atatggataa aggtgcatta gttcctgatg
    18361 aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc agatacagag aaaggcttct
    18421 tattagatgg ctttccacgt acgctagatc aagcaaaagc tctggacgca atgctaaaag
    18481 atttgaataa aaaaattgat gctgtcatcg atatccatgt tggtgaagaa atcttggtcg
    18541 aacgtctagc cggccgattt atctgcagca attgtggagc aacttaccat aaaattttca
    18601 accctacaaa agttgaagac acatgtgatc gttgcggtgg gcatgaattc tatcaaagag
    18661 aagatgataa acctgagacg gttaaaaatc gtctggctat caacatcaaa aatagtgaac
    18721 caatcttggc ttattataaa gaacaaggtt tgctaaacac tattgatggt gctcgtgaaa
    18781 tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa atagcacgat tgcccgtagc
    18841 cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa ttatgataga atatgtcagt
    18901 ccgacttcta gagaatcctc tagatgatcc attttgagaa ttttgaggtg ggaactgcag
    18961 tttccgccat tttatcgtgt gaaaatgcga aacaagtaca aggaggtact gtgcgtggca
    19021 aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa tgcaatgttt
    19081 aaagttgaac tagaaaacgg acaccaagtt cttgctactg tttcaggtaa aattcgtatg
    19141 cattacatcc gtatcttacc tggtgacaaa gtaacggttg aattgtctcc atacgatctt
    19201 aaccgtggcc gtattactta tcgctttaaa taattgtact ccgtaaaact acaggaggta
    19261 ttatcatgaa agtaagacca tcagtaaaac caatgtgcga acattgtaaa gtaattcgtc
    19321 gtaaaggacg cgttatggtg atttgcccag caaatccaaa acataaacaa cgtcaaggat
    19381 aattggaggt gctataaata tggctcgtat tgcaggagta gatattcctc gtgataaacg
    19441 tgtagtagtt tcacttactt atatctatgg tattggtaac actactgcta aaaagatctt
    19501 agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg aacaaacaga
    19561 tgctatccgt gcagaagtag ataaattaaa agttgaaggg gatcttcgtc gtgaagtgaa
    19621 cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc atcgccgtgg
    19681 attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag gcccagctcg
    19741 cgctatcgct ggcaagaaaa aataattctt aagtgaagga ggttaaaact tcatggcagc
    19801 aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta ttgcacatat
    19861 ccattctaca ttcaataaca ctatcatcat gatcactgat actcacggaa atgcattagc
    19921 gtggtcatca gctggttcat taggtttcaa aggaagcaaa aaatcaactc cttttgccgc
    19981 tcaaatggca gcagaaactg ctacaaaagc agcaatggaa cacggattaa agactgttga
    20041 agtaacagtt aaaggacctg gttctggacg tgaagcagca attcgttcat tacaagctac
    20101 aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt cctcataatg gtgcccgccc
    20161 tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg agttttatca gtaacgtcat
    20221 tgaacaagac acttttcgtt ttgaaagggg taaagataag aatgattgag ttcgaaaaac
    20281 caagaatcgc aaaaattgat gaagaaaaag attatggcaa gttcatcgtt gaacctcttg
    20341 aaagagggta tgggactact ttagggaatt ccctacgtcg tattttatta tcttctttgc
    20401 caggtgctgc catcactaat attcaaatcg atggtgtact gcatgaattc tctaccatca
    20461 aaggtgttag agaagacgtc actcaaatca tcttgaacat taaaggctta gcgctgaaaa
    20521 tgtatggcca agaagaaaaa acccttgaaa tcgatattac cggacctgct acagtaactg
    20581 ctggcgatat tatcgttgat agtgatgtag aaattcttaa caaagatatg tacatctgta
    20641 gtgtctctga aggagctacg ttccatgctc gcttaacagt gaaacctgga cgtggttatg
    20701 ttcaagcaga tgaaaataaa aaagaagata tgccaattgg cgttcttcca gtcgattcaa
    20761 tctacacacc tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt ggacaccgtg
    20821 aagattttga taaattgacg atggagattt ggacagatgg ttcaattgaa ccgttggaag
    20881 caatgagttt gtctgcgaaa atcatgactg aacatttgga tatttttgtg aacttaactg
    20941 atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa gaaaaaatgt
    21001 tggaaatgac gatcgaagag ttagacttgt ctgttcgctc ttacaattgt ttgaaacgtg
    21061 caggaatcaa tactgtacaa gaacttacaa ataaatctga gcctgaaatg atcaaagtcc
    21121 gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa attgcacgac ttaggtttag
    21181 gtttacgtaa agaagattaa aaccttttac gaaggaggga aaccaacgtg agttatcgta
    21241 aactaggacg cacatctagc caacgtaaag cgatgttgcg tgatttaaca actgatttat
    21301 taatcaacga acgtattgtg actactgaag ctcgtgcgaa agaagttcgc tcaactgcag
    21361 aaaaaatgat cactttaggt aaacgcggag atttgcatgc acgtcgtcaa gctgcggctt
    21421 tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc attgaatcag
    21481 ctttacaaaa actgttcaac gatattgcac ctcgttacgc agaacgtcaa ggcggataca
    21541 cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc accaatggtt gtcttagaac
    21601 ttgtctaaaa tcacactaat aacatcactt tatagatgat tcttttagag tgttatgatg
    21661 ttggaagata tttttccgag tctagctcag cgctgctcct caagcgaata tcgcttgggg
    21721 agtaagcact catcataaag tgttttttta taccttcaat tacctgtcat gacagatatg
    21781 tttgaaggca taaaaaaaca ttttaagcga gaaataattg aaaattttgt ctttttttga
    21841 tagtgttagt aggaacttta ggatgaagaa ggagtaatca tggagccaat tattgaatta
    21901 gaaaagataa attataaata ccagccagat gatcttcgcc ccgcattgaa agatgtctcc
    21961 tttacaattg ataaaggtga gtggatcgct attattggac acaatggttc aggaaaatcg
    22021 acacttgcca aaacgatcaa tggattgctt ctgccagaat cagggatcgt gaaagtggga
    22081 aatcaaatat tggatgaaga aaatatttgg acgatccgac agatggttgg aatggttttt
    22141 caaaatccag acaatcagtt tgttggttcg acagtagaag atgacgttgc ttttggtctt
    22201 gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc acttgagaaa
    22261 gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg gcaaaaacaa
    22321 cgtgttgcca ttgccggcgt ggttgcctta cgacctgata tcattatttt agatgaagca
    22381 accagtatgc tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa gaagatcaaa
    22441 gaagaaagtc aattgaccgt tatatctatc actcatgata ttgatgaagc agctaatgcc
    22501 aatcggatac tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc aaaagaaatt
    22561 ttttctgctg gtcctgaact gatcgactta ggtctagatc taccatttcc tgaaaaactg
    22621 aagagcgcac tgaaagaacg tggggttgat gtgcccagtg aatatatgac agaagaaagg
    22681 atggtggatt ggttatggac atccgttttg aacaagtaga cttcacttat cagccgaata
    22741 caccatttga acaaagagcc ttatttgaca tcaatatgac gatcaaagaa aacagttata
    22801 ctgcattagt agggcatacc ggaagcggga aatcaacctt acttcagcat ttgaatgcgc
    22861 ttgttaagcc aacaagcggt acagtccata ttggagaacg agatattcag ccggatacgg
    22921 ataataagaa tttaaagccc atccgaaaaa aagttggcat cgtctttcag tttccagaag
    22981 cacagctatt cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa aactttggtg
    23041 tcagcgaaga agaagcacta gtcctagcaa aagaaacatt agaacaagtt gggctggatg
    23101 aaagctattt ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt agagtagcaa
    23161 tcgctggtgt gcttgccatg aggccggaag tacttgtatt ggatgaacca acggcaggac
    23221 ttgatcccca gggtagaaaa gaaatgatgg agatgttttg gcgtcttcat aaggaacagc
    23281 agatcactat tgtattagtt acgcatttaa tggatgatgt agccaatttt gcagattatg
    23341 tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga gccgcaagag gtctttcaaa
    23401 acattgaatg gctaaaagaa aaacaactag gcgtacctac tgccaccgag tttgcagaag
    23461 aattaatggc taaagggatg aactttgcta ccttgccttt gacagctgaa gaattagcag
    23521 acgctatcgt ccattatgct ggaggaaatg cccatgatga ataaattgat atttggtcgt
    23581 tatatgcctg gagattcctt gatccatcga ttggatcccc gagctaaatt attagccagt
    23641 ttttatttta tcggaatcat ttttttagca aataattggg aaacgtacct tttgttggca
    23701 tttttcacat tactttctgt cttcttgtca aaagtagatt tgaaattttt tatccgaggc
    23761 attcgcccat tgatctggct aatcctcttc actgtagctt tgcaggtctt gtttacaagt
    23821 ggcggagaaa cttattggag ttggggtgtt ttccatatca ctgaatttgg tgtgatgaac
    23881 ggcctcttta ttttctgtcg attcgtgttg atcattttta tgtcgactct actcacgctt
    23941 actacaccgc ctttggaatt atcggatgcg attgagtact tgcttcgtcc attgaaagcc
    24001 gtacggttcc cagtccacga aatttcattg atgctgtcga ttgctcttcg ttttgtgcct
    24061 acattgatgg acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg tgttgatttt
    24121 ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc ctttgctgat tcctttattt
    24181 gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag aggctacaaa
    24241 ggtggggaag gaagaacgaa atatcgtgta ttgcattggc ataatagaga cacgatggtt
    24301 atcattgctt ttgtattatt aactgtgcta ctgatctttt tgagaggata aacgttcagt
    24361 gggccgctat ctattctttt cttgctgatc tgtttgcaag aaaggatgcg agcagtgtat
    24421 gacaagaaat attagggtct gtggcagact aaggtcacag gcctctttct tttatctgaa
    24481 aagaaagaac gaaaaaattt tataaaaaaa tcattattcc agataacctt ttaggaacaa
    24541 aggagagaaa aacatggtac gttacaaagc aatcattgca tacgacggaa caaattttaa
    24601 tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa aaacattaca
    24661 aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag atgccggagt
    24721 acatgcgatt gggcaagtaa tccattttga ttatccagag gagcgtccgt tagaacgaat
    24781 gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag tagaaattgt
    24841 ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat ttcgagtgga
    24901 tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc cttaccctct
    24961 tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg atttcacctc
    25021 tttttgtgca tcaggaagct cgatagagga taaagtacgt acgatctacg aggctaagat
    25081 ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg gcttcttgta
    25141 taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc gattgcctga
    25201 agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac ctacagcaca
    25261 tcctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttgtga cagaagtggt
    25321 cagcttcaag aaataagaag gaatttacga aaattacttc tcaaaatttt gcaaaatttc
    25381 ggcttatttt cgaaggagct acttctggaa caccgtgaat cagaggttgt gaaaaagctg
    25441 ccctgcatca agtaataaga acaaccaaac aaaaatagct c
//
LOCUS       DACEMK010000040        25134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEMK010000040 DACEMK010000000
VERSION     DACEMK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.040"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            367..1278
                     /locus_tag="IW715_001371"
     CDS             367..1278
                     /locus_tag="IW715_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1021983.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1495..1932
                     /locus_tag="IW715_001372"
     CDS             1495..1932
                     /locus_tag="IW715_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021984.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2027..2911
                     /locus_tag="IW715_001373"
     CDS             2027..2911
                     /locus_tag="IW715_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1021985.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3135..6302
                     /locus_tag="IW715_001374"
     CDS             3135..6302
                     /locus_tag="IW715_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1021986.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6322..6867
                     /locus_tag="IW715_001375"
     CDS             6322..6867
                     /locus_tag="IW715_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ1021987.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6895..7350
                     /locus_tag="IW715_001376"
     CDS             6895..7350
                     /locus_tag="IW715_001376"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ1021988.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7347..8105
                     /locus_tag="IW715_001377"
     CDS             7347..8105
                     /locus_tag="IW715_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1021989.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8191..8931)
                     /locus_tag="IW715_001378"
     CDS             complement(8191..8931)
                     /locus_tag="IW715_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1021990.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8992..10245
                     /locus_tag="IW715_001379"
     CDS             8992..10245
                     /locus_tag="IW715_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1021991.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10567..10794
                     /locus_tag="IW715_001380"
     CDS             10567..10794
                     /locus_tag="IW715_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1021992.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10917..11243
                     /locus_tag="IW715_001381"
     CDS             10917..11243
                     /locus_tag="IW715_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1021993.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11311..12024
                     /locus_tag="IW715_001382"
     CDS             11311..12024
                     /locus_tag="IW715_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1021994.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12334..13185
                     /locus_tag="IW715_001383"
     CDS             12334..13185
                     /locus_tag="IW715_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1021995.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13306..15171
                     /locus_tag="IW715_001384"
     CDS             13306..15171
                     /locus_tag="IW715_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1021996.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15219..16628
                     /locus_tag="IW715_001385"
     CDS             15219..16628
                     /locus_tag="IW715_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1021997.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16736..17437
                     /locus_tag="IW715_001386"
     CDS             16736..17437
                     /locus_tag="IW715_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1021998.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17596..18075
                     /locus_tag="IW715_001387"
                     /pseudo
     CDS             17596..18075
                     /locus_tag="IW715_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            18923..19498
                     /locus_tag="IW715_001388"
     CDS             18923..19498
                     /locus_tag="IW715_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1021999.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19498..19938
                     /locus_tag="IW715_001389"
     CDS             19498..19938
                     /locus_tag="IW715_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1022000.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            19954..20655
                     /locus_tag="IW715_001390"
     CDS             19954..20655
                     /locus_tag="IW715_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1022001.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21143..21568)
                     /locus_tag="IW715_001391"
     CDS             complement(21143..21568)
                     /locus_tag="IW715_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022002.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21942..22913
                     /locus_tag="IW715_001392"
     CDS             21942..22913
                     /locus_tag="IW715_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1022003.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            22945..24342
                     /locus_tag="IW715_001393"
     CDS             22945..24342
                     /locus_tag="IW715_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1022004.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24501..25109
                     /locus_tag="IW715_001394"
     CDS             24501..25109
                     /locus_tag="IW715_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022005.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         8579 a         4135 c         5303 g         7117 t
ORIGIN      
        1 tgtataaaaa tatatcataa taaaaacaac aaaaaaatgt ctggaaaatc gaacataaaa
       61 gtgtctgatt tgaatatccc agcagcggaa gaagaaaaac aaaatcgata acatttgttc
      121 taagaattag ttacgttaaa tacggaacgc aagatagact tgaaaaacgg taacaaaaga
      181 agtagtatat taaggtgtga tgtgctactt gacatttatt ctgctgagcg aactttctgg
      241 attgtgtagc cttatatcta ttcaggacgt atcgagcaga ttagtaaagg tgttcttgat
      301 gattttcatc ttgaacacct ttgtttttag caattcgcaa aaaacgaaaa gtaggagaat
      361 tgataaatga aaaaaagatt tttagcactt gccatcgtac taggcactgg cttattgtca
      421 ggctgtacga atgccggtga aaaaacagcc gttagttata aaggcggtac aatcagcgaa
      481 caagaagtaa tggatagctt gaaaaaaatg caaggagcag attccgctgt gcaacagttg
      541 atcgtttatc aagtatttga agataaatat ggagatgacg tatcaaccaa agaaatcgat
      601 tctcaatatg atcaaacaaa gaaacaattg ggtgattcat ttgattcaca attgaaatct
      661 gcaggttaca cagaacaaac attcaaagac agtatcaaac aaagtctagc ctttcaagaa
      721 ggtctaaaga aacatatcaa attgaccgat gaagatttaa aaacggcttg ggaatctttc
      781 catccagaag tggaagctca aattatccaa gtagcaagtg aagatgatgc aaaagatgtg
      841 aagaaagctg ctgataaggg agacgatttc tctaaattag ccaaagacaa gtcaactgat
      901 actacgacaa aagaagatgg cggaaaagtg aaatttgatt ccactactac aacggttccg
      961 gcagaagtca aagaagcagc ctttaagttg aaagatggtc aagtttctga tgtgatcact
     1021 tctaccaatg cttcaactta tactacggaa tattatgtgg ttaaaatggt gaaaaaccaa
     1081 aacaaaggca acgacatgga taaatacaaa aaagaattga aagaaattgc aacagacaca
     1141 aaattaagtg acagtacctt ccagaataaa gtaattggcg aagtattgaa agacgctaat
     1201 gtaaaaatca aagacaaaga ctttgaaaat gttttgagta cattcacttc agattctagt
     1261 acagcaagta gtaaataagc ggtataaata aaaaatatga actatttaaa tcaataaaac
     1321 tgttcatcta aatcttttta ttagaactaa atcaacagta gtttaattac tacttgattt
     1381 agttcttttt attttaaaaa ttttttttac ttagaatcca gcgaccctag atatttttct
     1441 gtataatctt aaataaagaa aacaatatca aaaaatgcac gtagaagtag ataaatggag
     1501 tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt tgtttttctc
     1561 acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat tcatcaattt
     1621 tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat tattcaaggc
     1681 cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat tcctctgatc
     1741 gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt gctatttttg
     1801 tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct atcattttct
     1861 tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat gttttatctt
     1921 gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat ttttttcttt
     1981 atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga aaaaagtttt
     2041 attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc aattagcgat
     2101 agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa aagcagggga
     2161 tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt ttgtcatggg
     2221 cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac atcggccaaa
     2281 tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag ggataccgct
     2341 agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat tggatattgg
     2401 aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc ctgaatcaat
     2461 caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt tcatgtcagg
     2521 aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg atggaaagaa
     2581 ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg gcggttttga
     2641 aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt atttaaaaga
     2701 ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag tagatgaatc
     2761 tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag ctgatgagga
     2821 agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga caatcaaagt
     2881 attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag gagttggcgg
     2941 gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca gccttttttt
     3001 tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata catattttaa
     3061 atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga
     3121 gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct
     3181 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc
     3241 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa
     3301 ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg
     3361 agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg
     3421 tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag
     3481 acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga
     3541 tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata
     3601 tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac
     3661 atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg
     3721 atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg acacttgatt
     3781 tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg
     3841 gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta
     3901 aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac
     3961 cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa
     4021 cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag
     4081 atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag
     4141 tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca
     4201 atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga
     4261 aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa
     4321 tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg
     4381 ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat
     4441 cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta
     4501 cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca
     4561 aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg
     4621 gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg
     4681 atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta
     4741 cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca
     4801 tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa
     4861 acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag
     4921 attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat
     4981 tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg
     5041 ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc
     5101 agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga
     5161 ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc
     5221 ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac
     5281 gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac
     5341 aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct
     5401 tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg
     5461 ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga
     5521 cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt
     5581 ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag
     5641 ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa
     5701 atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta
     5761 ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa
     5821 gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag
     5881 tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa
     5941 ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg
     6001 atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa
     6061 atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat
     6121 tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag
     6181 attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc
     6241 agatagctgg taattactca gcagagataa catggacatt agcagatgcg ccgacagaat
     6301 aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc
     6361 tctttactta tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc
     6421 gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca
     6481 tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac ggtctttaac
     6541 cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac
     6601 ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg
     6661 aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag
     6721 acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg
     6781 tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt
     6841 tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa
     6901 ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa
     6961 aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa
     7021 acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat
     7081 tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt
     7141 gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat
     7201 gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt
     7261 gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt
     7321 attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag
     7381 ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta
     7441 cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag
     7501 ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg
     7561 gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt
     7621 taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac
     7681 aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga
     7741 aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg
     7801 gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa
     7861 tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag
     7921 tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag
     7981 agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg
     8041 tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag
     8101 cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat
     8161 ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg
     8221 gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa
     8281 aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta
     8341 agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt gttttaaaga
     8401 agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt
     8461 gatactttca atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc
     8521 aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct gttctagcac
     8581 ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt
     8641 ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg
     8701 tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc
     8761 gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca actgattgat
     8821 ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca attctctgat
     8881 gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct
     8941 cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca
     9001 acagtgacac tttatttgac ctttactttg attttagatt taggtatcta tttattcttt
     9061 tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt ggtgacactt
     9121 ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt
     9181 ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct
     9241 atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat
     9301 cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa
     9361 agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat
     9421 tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt
     9481 cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt
     9541 tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa
     9601 tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat
     9661 gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg
     9721 ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa accgcaaata
     9781 caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa
     9841 aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc
     9901 ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat
     9961 catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatca
    10021 cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga gatggtcaaa
    10081 agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg
    10141 gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt
    10201 gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga
    10261 taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac
    10321 agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag
    10381 ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa
    10441 gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa
    10501 agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc tacgatcaca
    10561 aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat ccatcgaaaa
    10621 gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta
    10681 agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc
    10741 tcaagggaag aagacacgct actgcataca catggtggct acctaaatta ttgattttct
    10801 atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt
    10861 atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga
    10921 atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg
    10981 aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat
    11041 ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag
    11101 atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca
    11161 ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac
    11221 gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa
    11281 aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg
    11341 ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa
    11401 cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca
    11461 aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa
    11521 ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt
    11581 gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat
    11641 acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat
    11701 tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat
    11761 ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca
    11821 ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa
    11881 gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa
    11941 ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc
    12001 aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag
    12061 ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga
    12121 ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt
    12181 atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg
    12241 attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag
    12301 gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac
    12361 aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta
    12421 gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt
    12481 ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag
    12541 gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt
    12601 tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa
    12661 gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa
    12721 tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct
    12781 gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac
    12841 atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa
    12901 ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg
    12961 cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc
    13021 tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag
    13081 tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt
    13141 catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat
    13201 ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa
    13261 tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca
    13321 gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat
    13381 tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta
    13441 aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt
    13501 ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg
    13561 gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt
    13621 atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca
    13681 ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct
    13741 gaagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag
    13801 ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc
    13861 acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca
    13921 ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa
    13981 ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt
    14041 gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt
    14101 gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca
    14161 ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca tcggggattc
    14221 gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt
    14281 cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat
    14341 acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa
    14401 ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct
    14461 ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg
    14521 gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg
    14581 acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt
    14641 ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga
    14701 gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca
    14761 ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt
    14821 gatccagaag taggagaact agttgcacct gcagacggag agatcacgac tatttttcca
    14881 actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg
    14941 atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc
    15001 gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat
    15061 agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta
    15121 gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat
    15181 caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc
    15241 aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg
    15301 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
    15361 aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc
    15421 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
    15481 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
    15541 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
    15601 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
    15661 atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga
    15721 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
    15781 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
    15841 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
    15901 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
    15961 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
    16021 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
    16081 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
    16141 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
    16201 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
    16261 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
    16321 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
    16381 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
    16441 atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa
    16501 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
    16561 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
    16621 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
    16681 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
    16741 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
    16801 aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt
    16861 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
    16921 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
    16981 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
    17041 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
    17101 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
    17161 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
    17221 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
    17281 tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat
    17341 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
    17401 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
    17461 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
    17521 tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa
    17581 gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct
    17641 tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa
    17701 aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt
    17761 cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca
    17821 acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt
    17881 attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt
    17941 gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta
    18001 gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat
    18061 ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct
    18121 tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt
    18181 ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa
    18241 aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac
    18301 aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga
    18361 aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca
    18421 ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga
    18481 tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa
    18541 caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat
    18601 ttatcagtga aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta
    18661 tttgttgttt aaaataatac acgttattgg catataggac gaaatcccag tatcttctat
    18721 aagtaagtca tttcatatcg atgaaaacac gttatttatt tttgttttat ctataaaatg
    18781 gctgaaaaat gatttttttc taaatactat atatagtggc gtctatccta tataccacta
    18841 aaaaactaca aaatctagtc ttttatttta aaaaaacacg tgtagaatga agatagactt
    18901 ttgagttagg gagtgttttt ttatgaaact tactgttttt tgcggttccc gttttggtaa
    18961 taaagaaagt tacaaattta ttgcgcaaac gttaggaaaa tacatggcac aagaagaaat
    19021 cgaactggtt tacggtggtt catactccgg gattatggga gtgattagta aaacagtact
    19081 ggaaaataac ggaaaagtca caggtatcta tccgaatggg ctttttgaaa ctgaattgcc
    19141 tggaaaagat gttactacat ttattcctac agaaacgatc gatgaaagaa aggtattatt
    19201 atttgaaaag ggagatgctg tcctcgtttt ccctggaggt ctaggtacat tggaggaatt
    19261 ctcacaactt ctttcttgga tagcaatcgg tctgacgccc gacaagccaa tcggaatatt
    19321 gaatattggc ggttattaca gcggcttgca acaattatta gaaacttttg caaaagagga
    19381 ttttatggat aaaaaatggt tggatcaagt aatcttttcc aacaatccat tggaactggt
    19441 caatgaatta cgagcagtat ctattaaaaa tcagttatta ttgaaggagg caaaataatg
    19501 agtcaaatat atttagcagg accatttttt tcagaggagc aaatcgatcg agtaagtcgt
    19561 atcgaaaaag cgttggagga aaacaaaaca gtcacaagtt tctactctcc acgtcatcac
    19621 caagaaagca attatgaact attcagcgct ggatgggcgc aggaagtata cgaaaaagat
    19681 atggaagaac tgacaaatgc agaatttgtt gttgctatcc ttgactttga gcatcaaaca
    19741 atcgatccag gaacggctta cgaattaggt gtggcaacta tgttgaaaaa accgatgatc
    19801 attgtacaag aagagacggt acctacgaac ttgatgatta cacaaagtct ccatacgtat
    19861 ttaaaatcag accaagctgt acgtgaatat gattttgaaa cattgcctgt tgaaacttac
    19921 gtaggcgagt acttatagaa gaggaagggt tgtatgtcag tgattttgat ttcaggaacg
    19981 attggtgcag gtaagagcag tttgacggat atgcttgcaa aagagattga ttctaaacca
    20041 ttttatgaaa atgtcgaaga taacgaagta ttgccgctct tttattcaaa tcctgagcag
    20101 tacgcttttt tattgcaaat cttctttttg aacaagcgat ttttagcaat gaaaaatgct
    20161 ttagtgaacg atgacaatgt gctagaccga tccatctatg aagatagcct gttgttccat
    20221 ctaaacgctg atttaggtcg ggtaacggat atcgaagtac agcagtacga tagcttatta
    20281 gatacgatgc taaacgagtt agatgatgtt gctccaaaaa aacggcctga tttgatggtc
    20341 catatcaaag tttctttgga cacgatgcta gaacgtatca aaaaacgtgg gcgtgactat
    20401 gaacagctag aaagcgatga gacgttatat acctattatg aaacgttgaa cacccgctat
    20461 aatcaatggt atgaagattt tgatgtatgt cctaaaatac aagtggatgg ggataaattc
    20521 gattttgtag aaaatccaga agatgcaaaa aaggtgatcc gtcagatcca agaaaaatta
    20581 gctgaattgg aaggaaagcc tgctggaaaa tattttacgg ataaccagca tgcaaaaggt
    20641 caagaattag gctaaaaata aaaaaccaaa cgacgggaaa gaaaaattac tacgtgatgt
    20701 agaataagcc tttctatcgt ttggttattt tatattcaaa tatttgaaat aaataaatct
    20761 ttactctttg atttgtaaac tgaaatgatc cactgtttgt tttactgatg aagcttgatc
    20821 aaaggaagaa gctgagtttg aagttcataa aaatcagcca tagtgagttt tgcttggatt
    20881 tgtttatctt cttctacttt ttacataatt ttgaacagga taagattcaa atgttttaaa
    20941 tgaatctttg actagtttgt gacagttgtc gcatatgctc aagcttcctt ggtcattaaa
    21001 ttctgtatgg atatttttgt tgttattaca tgtacaataa tcaggactat cgatcatcca
    21061 tttcgtctcc tttacaatca tcttgcttct ttactatagc agaaaagaag gggagaaaca
    21121 aaagaagatc caacagacgg cactatagac tgtagatgtt gccatcctca tccttcaaac
    21181 ggtaattacc gatttttgta aatatgactg ttgtttcttt tttattttct tttcctgaaa
    21241 taatatattc gtcatctttg gtagtgatct tgatatctgt cagcactgtt ttttcttcag
    21301 atgattcaga agaatcagtt ccttgtgtct gatacgtgcg tgtccattcc atttttttat
    21361 ctgtaaatgt caactgtccc gcggataaat ccatatcttt acgtactttt agcgtatata
    21421 cactgttggt ggtatctgct ttttccgctt ttaagacaga agaatcggtt gtttcgtccg
    21481 aggattggtc agagccgcca aacaaatcac agccagtcaa tgtcaaaagt cccgcagaca
    21541 aaataaatag tacgattaat ttcttcaaaa tacttccccc atttgatagg ataaagaaat
    21601 tataccattg gaatgtaaaa cttcaaacgg atggaagaga aaaacgcaca aaacacccac
    21661 aaatagagta aattttagaa taaaatttta aaaaggcaaa aaatccgcca ctttcagaaa
    21721 gcgttttaaa aaagtgttgc aatcttttaa aaaaatgcta tattaaaaat gaaccggttc
    21781 aaatacttgt ttaagtataa ttaatagtga gaggaagttg ctctactgaa gactttagtt
    21841 atatggatat tgatgacaag caacactatg caaagaatgc ggtcatcata atcgaacaac
    21901 aagaaagaga aatcaaatgt aactggcagg agtgagaatg tatggcatcg atccgtgatg
    21961 tggctaagat ggctggtgtt tcagtgggaa cagtttcccg ttacttgaat ggtcagcaac
    22021 tgaaggaaaa gaacatgaaa aaaatagcag aagcgatttc agcgttggac tataaagaaa
    22081 acatcattgc taaagggttg aagaataatc gaagcttctc tattggactt ttgataaacg
    22141 ggatttccag ccgttttggg tcggaagttg tctcagggat cgaaagagta gctgaagaaa
    22201 atgggtatag cttattatta agcgggtttg ccgatcagcc agaaaaaatt gaacaaaaaa
    22261 ttgaatacct gatgaagcat gtgatcgatg ggctgattgt cttcttatcc gaagaagagt
    22321 ggtctggatt tgaaatgctc acaaaaatga gtattccagt tatcgcattg aattgtccag
    22381 aaggaccagc aggtattgat acaatattag tgaatgatcg agaaagtgta gcaaaagtga
    22441 tcgctcatca tggtgaacaa ggacataaaa aaatcggatt tattgctgcc actcaaacag
    22501 actacgtagc aagagaacgt ttagcaggag ccaaagaagc ggtccaaaat gatccatcga
    22561 tccaattaga ggtctttaca ggagactatt ctagaaaaag cggctattac ggggcaaaag
    22621 cattattaga aaaaggggca actgctattt ttgtcagcaa ttacaatatg tcgattggtg
    22681 ccatcgaatg tttcaatcaa gaaggtgtca gaatcgggaa ggatattgcg ttcagtcact
    22741 atgattattt agataaaaac gatctgagta tccttccgaa aatcacgatc acgccaccaa
    22801 cagataaaat cggggaacga gcagcagaac agctgttaaa acgaatcaaa aaagaagagg
    22861 aagaaaatga aaaagtgatt gtgatggaaa atatcataca tggaatcaac taaaacgaac
    22921 taaaaaagaa aaagaggtgc tttgatgaaa aaatatcaat ttccagcaaa tttttggtgg
    22981 ggttctgcag caagtggccc acaaacagaa ggaaggattt caggcgatgg aaaaggagag
    23041 aacatttttg attattggta caaaaaagaa ccgcaaaagt tctttgatca agtaggacct
    23101 gaaaaaacct cgcaagttta tacgaagtat caagaggatg tacagttgat gaaacagact
    23161 gggcataatt catttcgaac atctatccaa tggagccgtt tgatacctga tgaatctgga
    23221 acaatcaacg aagaagccgt tcgtttctat catcgttatt ttgatgagtt gattgaaaac
    23281 ggtatcgaac cctttgtgaa tctgtatcat ttcgatatgc cgatgtatct gcaagaaaaa
    23341 ggcggatggt tgaaccgaga aacggttgaa gcctatgaaa aatatgccaa aagttgtttt
    23401 gaattgtttg gttcgaaagt aaaaaaatgg tttactcata atgaaccaat cgttccagtc
    23461 gaagcaggtt atttatatcg atggcattat ccagaagagt cagatatgaa aaaagcgatc
    23521 caagttggct atcatgaagc gttggcttca gcgttagcca tcaaatctta ccatgaaatg
    23581 gagcaaggtg gtgttattgg tatcatcttg aatctgacac caagctatcc aagagacgaa
    23641 tcaaaccctg aagatgttaa agcagcgcaa atagcagatg cgttcttcaa tcgttcattc
    23701 cttgatccag ctgtaaaagg cgagtttcca agtgaattga ttgagatagt aaaagaactt
    23761 gatctcatgc ctgtgatcca tgcagaggat ttgacggtta tcaaagaaaa tacagtagat
    23821 cttttaggta tcaattatta ccagccacgc cgggtaaaag caaaagaaac accaatcgat
    23881 actaaaaacg gtccaatgcc tgaggattat ttcgacaatt atgaaatgcc tggtcgtaaa
    23941 atgaatcctt atcgcggatg ggagatctat gaacaaggaa tctatgatat tttgatcaat
    24001 gtacgagatc attatggaaa tatcgactgc tttatttctg aaaacggtat gggtgtagaa
    24061 aacgaagcac gatttatcaa agaagatggc atgatccacg atgattatcg gatcgaattt
    24121 gtccaatctc atctgaaata tgtccatcaa gccattcaag aaggtgccaa ttgtttaggc
    24181 tatcatatgt ggacatgtat ggataactgg tcgtggacca atgcatataa gaatcgatat
    24241 ggctttattt cagtagactt agcacaagat ggaaaaagaa caatcaaaaa atcgggtcat
    24301 tggttcaaag acgtatcaga taaaaacgga tttgaggctt aagaaagaat agttgagagc
    24361 aaatgcgtga aattctccat tcttaccatt ttgtgaaaaa gaagttcaat tatttacgat
    24421 ctttttcaac ttttttgtct ttctatttca ttttttgtat ataatagaat agtcaaaatt
    24481 aaataggagt ggaggaaagc atgaggaaaa aaagaccgtt tcttctcttt tggttattcg
    24541 gttttttctt actgcttatt ttatccgcag cattttcagg aaaaatgaca gcagatcttg
    24601 ctcagaaaga aacagaaaaa atcgtagcaa gcaaagaatc agtagataat ttcaaaaaag
    24661 aattctcaac ttattggtgg gcggagtata aaataactgc caattacgaa aaaggcgaat
    24721 tggacattta tttgccaaaa ggaaaagaag aaaatcaatt tattaatatc gatgacgtac
    24781 ttagtgcgat ttctctcaga caatatcaat ttaaagacaa gcatttgacc gttttgcttt
    24841 tgactcatga tggcgacaaa gtagcccgtt tgatcaccgc ttattctgaa aaagtagaca
    24901 aaaaagtggt cgccgatcgt ttagcggaat ggaaaaagac aaatgaacaa gtcaagaaaa
    24961 caactgtctc ttctcaaaaa aacgagtcga caggaacaag tccttcagat tctggcagtg
    25021 gacaaacgat aacgggaagt gaagaaaatc aagcctcaat tattacgaat gagtcatcac
    25081 aaggacaata tcaatcctat ggacaatgaa aaacagaggt tgtgaaatca gcgt
//
LOCUS       DACEMK010000041        23860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEMK010000041 DACEMK010000000
VERSION     DACEMK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.041"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(196..501)
                     /locus_tag="IW715_001395"
     CDS             complement(196..501)
                     /locus_tag="IW715_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1022006.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(526..957)
                     /gene="ruvX"
                     /locus_tag="IW715_001396"
     CDS             complement(526..957)
                     /gene="ruvX"
                     /locus_tag="IW715_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1022007.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(954..1220)
                     /locus_tag="IW715_001397"
     CDS             complement(954..1220)
                     /locus_tag="IW715_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1022008.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1475..2200)
                     /locus_tag="IW715_001398"
     CDS             complement(1475..2200)
                     /locus_tag="IW715_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1022009.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2561..2631)
                     /locus_tag="IW715_001399"
     tRNA            complement(2561..2631)
                     /locus_tag="IW715_001399"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2597..2599),aa:Gly,seq:ccc)
     gene            2748..3500
                     /locus_tag="IW715_001400"
     CDS             2748..3500
                     /locus_tag="IW715_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1022010.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3516..3995
                     /locus_tag="IW715_001401"
     CDS             3516..3995
                     /locus_tag="IW715_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1022011.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4246..5826)
                     /locus_tag="IW715_001402"
     CDS             complement(4246..5826)
                     /locus_tag="IW715_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1022012.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5819..6561)
                     /locus_tag="IW715_001403"
                     /pseudo
     CDS             complement(5819..6561)
                     /locus_tag="IW715_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(6737..7513)
                     /locus_tag="IW715_001404"
     CDS             complement(6737..7513)
                     /locus_tag="IW715_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1022013.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7761..8597)
                     /locus_tag="IW715_001405"
     CDS             complement(7761..8597)
                     /locus_tag="IW715_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1022014.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8608..9537)
                     /locus_tag="IW715_001406"
     CDS             complement(8608..9537)
                     /locus_tag="IW715_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1022015.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9729..10082
                     /locus_tag="IW715_001407"
     CDS             9729..10082
                     /locus_tag="IW715_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1022016.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10156..10812)
                     /locus_tag="IW715_001408"
     CDS             complement(10156..10812)
                     /locus_tag="IW715_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1022017.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11038..12972
                     /locus_tag="IW715_001409"
     CDS             11038..12972
                     /locus_tag="IW715_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1022018.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13058..14281)
                     /locus_tag="IW715_001410"
     CDS             complement(13058..14281)
                     /locus_tag="IW715_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1022019.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14275..16173)
                     /locus_tag="IW715_001411"
     CDS             complement(14275..16173)
                     /locus_tag="IW715_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1022020.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16167..17285)
                     /locus_tag="IW715_001412"
     CDS             complement(16167..17285)
                     /locus_tag="IW715_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1022021.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17368..18201)
                     /locus_tag="IW715_001413"
     CDS             complement(17368..18201)
                     /locus_tag="IW715_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1022022.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18194..19111)
                     /locus_tag="IW715_001414"
     CDS             complement(18194..19111)
                     /locus_tag="IW715_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1022023.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19338..20462)
                     /locus_tag="IW715_001415"
     CDS             complement(19338..20462)
                     /locus_tag="IW715_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1022024.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20645..21553
                     /locus_tag="IW715_001416"
     CDS             20645..21553
                     /locus_tag="IW715_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022025.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21694..23307
                     /locus_tag="IW715_001417"
     CDS             21694..23307
                     /locus_tag="IW715_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1022026.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7432 a         4656 c         3993 g         7779 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
      241 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
      301 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
      361 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
      421 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
      481 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
      541 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
      601 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
      661 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
      721 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
      781 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
      841 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
      901 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
      961 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
     1021 tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggccgg gtctccagaa
     1081 agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa agcccgataa
     1141 acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg
     1201 gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt ccagcttacc
     1261 ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc
     1321 gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc tttatcaaaa
     1381 gtactctttt cataaagtat acgctatatt tctttgaatg tatgttttta cacaattttt
     1441 tcataatatt cataaaaaat tcgaatagct gctactaatt ttttgttttt gttgcttgtc
     1501 ctgtctcata attgatcgtg tagccatctt gaacattgta gataaagaca ttgaattcaa
     1561 tttttttgtc ttcaatgctt tctgcttcca attgcactcc cctggctacc agttcattcc
     1621 cctcaaaatg aggagtgaca cggtagcgta catgatgatt tgtttctttg atatatgccg
     1681 caatatcatt ttcgtgagca agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca
     1741 agtttttaat gttgttattt tgaccagtca gctggaaacc aattaaatgg ctgcgattat
     1801 ataaccattg cccgtcactt aatttctttt gtttccaacc tgtcgggtca atatacaaag
     1861 gctctctctt ttcagtaggg aacaaatctt ttccaagcat agcattggct tgtcctactc
     1921 tgttcaattt atcaatattt gaatagcttt cccatgttcc tttggaaagg ctaagatcct
     1981 cttttgtaaa ttccggctga ttcccattga tttctatttc ttgatgtttt ccatcatatt
     2041 ctggcacatc cgctacttga gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct
     2101 gaaagatatc tgaatctttt tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag
     2161 cgacgatccc cacgatcgcc ttggttgttt ttttgctcat ttctttactc ctttactaac
     2221 agctccctaa atcttaacat atgttcccct ataaataaat actagaaaga aatagaaaca
     2281 gcaaaaaata aaacctgttt taagatggct taaaaataca aaaaagagac ggattcctcg
     2341 tctctaatca gtcaccactc tatctgtaag gtatttatta ttattcaaaa attttcttca
     2401 tactcaagac aatacactga tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat
     2461 ctgcggtaat aagtccatcc aagttaaaaa cataagaagt tgtttttgct tggctgttct
     2521 tattacttgt tgcagagcag ctttttcaca acctatttta agcgggtgac gggaatcgaa
     2581 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg caatacaaaa
     2641 acagtatacg cctcttttat tccttcctca agttttttta ttctttacgc ttctggtatg
     2701 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     2761 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     2821 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     2881 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     2941 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     3001 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     3061 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     3121 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     3181 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     3241 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     3301 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     3361 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     3421 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     3481 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     3541 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     3601 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     3661 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     3721 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     3781 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     3841 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     3901 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     3961 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     4021 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     4081 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     4141 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     4201 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     4261 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     4321 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     4381 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     4441 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     4501 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     4561 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     4621 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     4681 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     4741 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     4801 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     4861 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     4921 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     4981 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     5041 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     5101 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     5161 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     5221 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
     5281 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
     5341 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
     5401 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
     5461 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
     5521 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
     5581 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
     5641 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
     5701 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
     5761 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
     5821 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
     5881 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
     5941 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
     6001 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
     6061 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
     6121 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
     6181 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
     6241 ttgtgtgtct aattcatagg cagctgaaat caagtcccaa tattctccag ccgtcaattg
     6301 taaaaaaata tctggggaat caggcacata accgattttt tgtttgatac tcaatcgatt
     6361 ttccgtcaat ttcattccat ctactaaaat cgttcccgat gatggttcaa taatactaac
     6421 taaactttta atcgtagttg attttccggc accattatgt cctaaaaatc caaaaatttc
     6481 cccttgtctt atcgagagag acaagtcttt taatgcttgt tttttcccgt aacttttact
     6541 tacattgatg aattcaatca tttctgtact ccttttgttt gttattactt tttattgtac
     6601 ccttcgttat tggtggagta aatgtcaaaa agagtgcata acaaaaaagg atagaaacga
     6661 ctaggctgct acttgggttg ttctcttcta caggaaaaca ctcttctaac aggtaagata
     6721 tgagtctttg ttttctttaa aatgattcat tttctatctt ttcttgcata aaattcttta
     6781 gtttatcaat acttctcttt cccatctcta gttgttctcc atttgcaaat gttactattt
     6841 cgtttactct gctaatagat gtaatgtttt cagtattaat gatataagct tttaaatctt
     6901 tacataaata ctcatattcg agtgattttt tatatttttt aattgtgccg tttacgagaa
     6961 tctcttcatc tttcaatact aggcctattt tatttttata acctggcatg ctcattagat
     7021 aaaggatttc atcgtaatgg atatagtgat cgctattacc ataacggaca gttgtccacc
     7081 tttttttcgt agcatgttga acaggatagt tttctttttc tttcaataag taactttgga
     7141 tgcttttttt atttttttca taagtgaatt cttttatcaa ataaccacta ggcgtaatct
     7201 ttcgattaat aacagttatt gctttattcg gatgagacgt tagataaaca attttacact
     7261 tttcattctt ttctctaagc atttcgccta gttcgatacc gctataatag gcgttcaaat
     7321 cgatgtctag gaaaaaaata tctgaggaat agatttcatt atttttaatg aattcaacaa
     7381 attgtataac gttcaatccc tgaacgatct caaatttcca aggacttaat aaaattctgg
     7441 attcttccaa aatttctact agcatttttt caaaaaccaa actatcatcc aaaataaata
     7501 tacgcgttac cataaaaagt acctccactc gatattttac tatcaaaatc atttcataca
     7561 acttttcaac atacatgtta ggaataaatt gtaattctct tcacaatctt ttccaacacc
     7621 tttgaaaccg atatcaaata ctttcaggat gctgcttcta aaataaatat caaacaaatg
     7681 aatacatcat gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct gtctgagcta
     7741 tgtttattta ttttgttctt ttaaaaagaa cgatacacat actgattttt cggagcagag
     7801 actttctcag cgtttgtaac aactacagat gggatttcac gcttgaatgg ttgataatag
     7861 tctgattgga tggttccttc tattttatac cattcatcat ttttaaccgt tactccttcc
     7921 ggcatatgcg tcaacaaacc aaagactcct gaatctgcca cacaatggat aatgccgaat
     7981 cggaacaaga agatatctgt attgctatcg ttcggtgttt ggtaaacaaa tccttcatag
     8041 ctgatttttt tgccaatgaa ttcgctaggg taattataaa ttagctccat aacttctaaa
     8101 tagttttcat ctgtgatctt gatagtttgc ttatccccat acttttcttt caactcattc
     8161 atttggtcta agtagtctga tttgttgaaa taaatactgg tatccggctt taagtattgt
     8221 gtctgcatct ccggatcccc tactgattct ttacttactg ggaaattaaa tcctttggct
     8281 tctacgatcg tagtgtcgag actaactgtt ggaaacaacg tccccacgat tactggcaaa
     8341 gctagcagta cataagcgat tccacgttga taaggttttg acaacccatg gtcatgatcg
     8401 tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc acaaatacag ctgaactaaa
     8461 gccaaaagag cagataaaat cattgaaaga atagctaagt agcgataatg gacattgata
     8521 tattgatcga gtttgccaga aacttgcaag tacatcatca attcgaaata cccagataaa
     8581 atcaaaaacc gcagcatatt ttccctccta aataaataat gtgaatccaa taacgctaag
     8641 ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag
     8701 tagatttttg atatcgacca ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc
     8761 gaacagactt aacaaagaag atcctataaa cgcatctgct tctgagcaaa gagacatcgt
     8821 aaaggccaat aacagcatga tgataatggc taaaatcttt gttgagccga gcgttagcat
     8881 aattctagtt ggtaaataag tctgcatcgc agctgcgatt aatccgccaa tgatcaaata
     8941 acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag tttgatggat
     9001 cagctgattt tttggttcat tcttctcact ttgttcttct tcatggacat gatgatggtg
     9061 attttctgct actttagacg tgattttttc tttcaataca gcgtcttttt gaaaataggc
     9121 aagccagaca ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc agcttgccat
     9181 ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc acgattggat tgatgatcgg
     9241 agcagtcagc ataaaagcaa acgctgtgtg aactggtacg ccttttttaa caaattgatg
     9301 aacgatcggt acgatgccgc attcacatga aggaaagaaa aatcctaaga tcgaaccagt
     9361 aaaaatagag aagatacgat tttcaggaag ccatctcttc acacgttctg gggtcaaaaa
     9421 tacttgtaag gcgcctgaga taagacagcc taataatacg aaaggtaatg cttcaataac
     9481 gatcgataag aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag aaaacatgag
     9541 tttcctcctt tttatttcta gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa
     9601 acaagttgct ctgtaaattt ttacacaact cttatttttt accaaagcac ttcaattgac
     9661 tttttctgtt attcccatta aattaaggtc tcatacatat cacttctatc ataaaaggga
     9721 gaataaaaat gaaacaaacc actaacttga ctgaagttca agacatactg caacagtcat
     9781 ccgttgccgt catttatctt tcgatgccta attgcagtgt ttgtcacgct gttcgtccac
     9841 gactagagga acttctcaca cattatgata ttccggcact tcatttggat gcatttgaaa
     9901 caccagaagt agctagtaaa tttgaagtgc tgactgcgcc agttgtcctg atttttcatc
     9961 aaggaaaaga agtctttcgg caagcacggt tcatcgactt taaaaaaatc cgttatctac
    10021 tagatgaact gacagcagaa gatgattccc tttcttatga agaaatcttc cgaacaaaat
    10081 aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa agttttgcct tcatttgttc
    10141 aatcgctttt tatttttatt ctgctgtatt ttcggaatgc gcatttgcgt cagaatgcaa
    10201 gaaatagatc aaggtttgca attcattcgt caaatcaaca ttttggatca atacatctga
    10261 cggagcattc aatcgagcag cggtaaagtt caagattcct ttgattcctg cttccgttaa
    10321 ctgattgact acttcttgtg ctctttcagc gggtatcgtt aaaattgcta cttcgatttg
    10381 ttggatcttg atttgttctg acatatcttc cattggataa acggggatac cgtctacgat
    10441 gcgcccaacg atttcctcat tcacatcaaa agcacaactg atacgtatgc tattactttg
    10501 atggaatttg tattttagta aagcacttcc caagttccct acaccgatca gcgcaacatt
    10561 tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc ataagatctt ctacatcata
    10621 accataccca cgttttccta attcgccaaa ataagaaaaa tctcgacgga tcgttgcact
    10681 atctacttga acggcttcac ttaattcagt tgaagacact tttttctttc cagcattatt
    10741 taaaatcctt aaatagcgat aatacaaagg cagtctttta gctgtcgcct ttggaataat
    10801 atgatctttc acgaaatgac ctccacgtat tgtctaatta catctttgat ttctccatca
    10861 tagcagtatt tataggaaaa aatcaattcc attgctcaga tattgtgaaa ttttattctt
    10921 tattcattta tttatcattt ctgcaaatct cgcagtataa tggaatttat ggtagactac
    10981 taattgaaaa atagtttttt atttgaggag aattcacaaa gtagaaaaga ggattccatg
    11041 atattattac aagccaatca gattgctcgc cattttggtg cagaagtctt atttgaaaac
    11101 atacatttag aaatcagtac aggcgctcgt gtcgctttag ttggcagaaa tggcgctgga
    11161 aaatcgactt tattaaagat cattgctgga attgaagcac ctgatgaagg aaagatcgct
    11221 aaaaataaaa cagcgactct cggatacctc gctcaggaca caggacttga ttccaatgaa
    11281 acagtttgga acgagatgct gaaagctttt gaacaagtta gacaaatgga agagcggatg
    11341 cgcgagctag agattttcat tagcgaaggt acgccagaca cacctgctta tagttcttat
    11401 ttaaaagaat acgaccggct gcaacacgat ttttcagatc gaaacggcta tggttatgaa
    11461 aatgaaattc gttccgtatt gcatgggttt aaattcgatt cttcatttta tgatcagaaa
    11521 atcgatactt tatcaggcgg acagcgcaca cgtttagctt tagctcgcat gcttttgcaa
    11581 caccctgaca ttttgattct agatgaacca acgaaccatt tagatatcga gacactttcc
    11641 tggcttgaga attatctgca aagctatact ggtgcattat tgattgtttc ccatgaccgt
    11701 tattttttag ataaagtagt caatgaagtc tatgaattaa gcagaagaaa aatgcgccat
    11761 tacaaaggta actattcgaa atatctagat ttgaaagcag aacagttagc aagtgaatgg
    11821 aaggcttatg aaaagcagca aacagaaatc cataaattag aagacttcgt tgctcggaac
    11881 cttgttcgcg cctctaccac aaaacgagcg caaagccgca gaaagacatt agaaaaaatg
    11941 gaccggctgg atcgtcctca aggggatgaa aaacaagcaa actttctatt cagtatagaa
    12001 aagccttctg gaaatgtagt cttgcaaatc gaagatgccg ctatcggata tgataatgtc
    12061 accttatctg aaccaatcaa tctagatatc cgtcgtcaag aagcgattgc tctagtaggt
    12121 ccgaatggta tcgggaaatc gacgttgctg aaatcaatca ttcaccagat cccttttatc
    12181 cgtggaaaag aaacgctagg gacaaatgtc accatcggct attacgatca aggtcaagct
    12241 gatctgcata caaacaagac gatcctagca gaattgtggg atgagcatcc taccactcca
    12301 gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt ctggcgaaga tgtagaaaaa
    12361 ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg ctttagccaa gttagcgatg
    12421 aacaaagaaa attttttgat tttggatgaa ccaacaaacc atttagatat tgacaacaaa
    12481 gaagtattag aaaatgcatt gatcgattat gacggcacac tgctttttgt ttcccatgac
    12541 cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac tctctgaaaa tggcagcaaa
    12601 gtctatctag gagactatga ttattattta gagaaaaagc aggaagaaga agaaatcgcc
    12661 gaattgttgg cacaagatga gccagaatct gctcctgctc ccacgaacaa atctgattat
    12721 taccaaagca aagaacagca aaagcttatc cgtagccttc aacgaaaaat tactcaggtt
    12781 gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt tggaacaaga aatggtcgcg
    12841 ccagaaaacc tggaagatca cgtaaaactc aatgagctga accaagattt agaagctgct
    12901 agacaagatc aagaaacaaa attgacggaa tgggaagaac taagcttgga attagaagaa
    12961 tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat gctgttttgg tttttctttt
    13021 tttattttat caaacaaaaa ttccatattt acctttttca ccaataagaa gaccaattac
    13081 tagtcattct cactaaagct tctaaataat agtaatcacc ccaaatacaa ggtgtattta
    13141 ccccattatt tttgggctta ttataaacag actgtgttaa aaagccatct tcttcttccg
    13201 ttgtagcata tctttctttc aattgtcgca taattttcca aacagcagac tggtataagt
    13261 tcttttctgt tttgtctaat agacctgatt ctattaattc taataatcca caaaccatga
    13321 tcaccatact agatgtatct ttttcttctc tgctagaatc atcaaagatc aaatcccaat
    13381 aacaaatggt atcttctggc aaccgattca taaaataatt ggctaatatt ttgcttaaat
    13441 caaaatattt ttgttctttc aaataacgat aacttaatgc aaaaccgtaa atcccccatg
    13501 cttggcctct agcccaacag gaattatcac tataaccttg atgcgtactt ccatatcttg
    13561 gttttccatc tttgacatcc atataaaatg tatgataagt tgaaccatct tctcttacta
    13621 aataatctgc tgcattttga atatgagcct gagcagcttt tttatatttt tgatttccag
    13681 tttcttcact cgcccaatac aataatggaa gattcatgca acaatccatg atcattctac
    13741 cttgttgtga aggatcacta ggatctcccc aagcttgtat aactcctgcc ttctcccaaa
    13801 aacgctgaag taatgtttct gcagcatcaa ttgaagcttc cttcattttt cgatttcccg
    13861 ttattttata atctgctacg gttgataaag aatacaaaaa accaagatcg tgatgattag
    13921 tgataatttg acagtcaatc ctgtggataa aatctatatc tgctttttca gctgtttgtt
    13981 gaaataaagg atcacccgtc ttctcaaata acaaccaatt aattcctgtc caaaatcctt
    14041 ctgtccaacc aatattgtct cttaatggat aacgtccttt tataggacac tcgcttggga
    14101 attgatactg actgttactg atacgtttga ttaacctctc cgtgtgtctg cgacaacgct
    14161 cgatctcatt attccattct ttttccatta attctttata acattcattt atccattttt
    14221 ctggatgtat tatctcttca aacatcactt tatcaagcat ttccccacca gcctttagca
    14281 taattttagt ctttttttat tgtttttatt ttggatagtt agcccggttg tcccatcaaa
    14341 atcaattttg ggacgattgt tccatacttc taaactataa tcccgatctt gatgagcaac
    14401 cactcctgtt ccgagccaat gggttccttt tccaaatgta ccctctaatc caggaataaa
    14461 agtgttttcc catgtcatca agtttgtatt gggcaaaccc ataatagctg tggattttcg
    14521 tttaatagta tcgtcactta aacagataat acctgaaaag ccagcttcac tcaccctata
    14581 gttttccttg tcttcttcta tgattattcc ttctttctca ggagaacatt caatagcaaa
    14641 ccctgtctca tatagaacat attctgtatc taattctact ttgtgtacgc gaatctgcca
    14701 gccttcaaaa ggaataagcc aagttctgat ttttgcctgt aaagtaggtt cccatgtaga
    14761 ggcaagtacc tctttcttac atacaatgtc tcgtacatta tttcgaacga tgaactgtcc
    14821 ataggttttg atgctctcat tcttaaaact ggtcgtgata tatgtgtccg ctcgctgaat
    14881 ccctagcatt gaatccattg ccatttctgc tttcaattga ttcgctcgcg gtatcgaaaa
    14941 accaaattga ctagaataag caaattttaa atatttttca ttggtcaatg tatggaaatt
    15001 tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca ttatcatgtt gtatgagcaa
    15061 attggcttct gaaagaagtt tagttccttc acgaataggc ataggcattt ctttagccgt
    15121 ccaatactca tggtcttcag gtagcgccaa caataaaaaa atcttattca gccataatgg
    15181 agataacgta ctattatagg gttcagtcaa tgtcaattga gggtaggtat aaccaatatt
    15241 caggatacct ttttcatcaa atatttcttt ttgcatccac catcttatat tccgattgac
    15301 taaccccttg atttcaccta atttaaaagg caaaagatcg gcccacaaca tagctgacca
    15361 aaagcttgaa actgcaaaac gataaatcat gcttcgacca aaaggcacat ttgcaccatc
    15421 ttccgcaaaa aaatgaatat agtctctaga aaaatcaata gctcggttct tatatatctg
    15481 gctaatgggc ttttctggtt ctagtacgct ataaatcaaa ccataataat gaaatgcaaa
    15541 aggattataa taatcttggc gacctcttga actgtctcga taccagccat tttgaagata
    15601 acaattttct atattatcaa atgcttcttt cagtgctgat tctttaactg gataaccaaa
    15661 tttttggata attgttccga caattatctt aaaaaattgc caattaccat ctgcacattc
    15721 atattcaaaa atttgcgaga accaatgaat aatctggctt ttttctcctg tatctaattt
    15781 tttccataat aattgttcat gatgatataa cgctaataac attgcgggca tttctaccat
    15841 acgctggtct ctttcatgta tttctcccca ataatcggga tgggaaggat ctgttccagc
    15901 ttttattcct gatagaatta atttcaacca tcgttcatct aattggtcaa ttgcgggccc
    15961 cgctccccat aaaacccgag agaatccttc cattcctgca attttatttc catatcctac
    16021 cccttgatgt atataatcaa tttgtgcgcc acttgctcga taaaacggct ggagtggttg
    16081 atttatcttt ttaaataagt caaagtaatc ctgtctggtc cttaatagtg gtactggatg
    16141 tttcattgta ttttctcctc tttttactac caaatagcta attccgactg ttctaatcga
    16201 agtaagcctt caataaaaaa ataatcagaa tagatcagtg gcacattata agtcagttct
    16261 ctatggtatg cttctgctcc gcctccaatg atcccatctt catccatatt ccaatttgca
    16321 tagtgctttt ctgtcttttg gagtatattg atagcagctt catgatacat ttttgatcgt
    16381 acagaggaaa catatcctgc taattccaga agaccgcatg cagcgcatac tccagcactg
    16441 gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag gaatataacc agttttttcg
    16501 attttctgaa taaaatttac tgctatcttt tctgctatag ctagatagta cggatttttt
    16561 gtgtaacgat aacttaacgc gaatccatat aaagcccatg catttcctct gctccaacaa
    16621 gaattctcat tgtatccttg cccgccaagt aaccgaatat atttccctga ttctgcatca
    16681 aattctgcga tatgcccggt tgatccatct ttcctcacta gatacttagc tgttgtatct
    16741 gcatgctgcc gagcaatttt agagaacctc ggatcaccag tttccctcga agcccaatac
    16801 agtaatggta aattcatcat actatcaata atcatccaac caggtttatg gtcattccat
    16861 gctactaaat agttccctga aatattatag cgtccagcaa gtaccgtagc tgcatgtaac
    16921 cctagttgtc gactatgttt gttttgagac tgttgataat gtgcaatagc cgatggtaag
    16981 aataaaaaac ctacatcatg atgaacgtct gaaaaatggt acagggcaga ttctaataat
    17041 tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt ttgtcttgtg aaagagctgc
    17101 cataaaatac cactccaaaa accattggtc caccagtcga ttcctctgtc ctttattacg
    17161 tctgtatatt ttccgttgta agaaatatat ggaattttat tccccattct gtctaattcc
    17221 gcatctatct tttcagaagc tacattcaaa atttttcttg accattcctt cgtctgttca
    17281 ttcatggata tcccgccatt cttttaattt ttatgaatgt ggagttgctc ctagaacgaa
    17341 ctagatggtt aacattcgtt atttttttta cttatttgca gctccaatgt cattctcctt
    17401 aaatttagac tgcgcccaca cataaatcaa gaccattggg atcactgaag ttgctaaaat
    17461 tagcataatt gcgccataat taattggctc acctgtacta gaaaccgtca tatttttgat
    17521 aattaaggga accgtatatt tatctttatc tgaaagcatg actaatggaa atagatattg
    17581 attccaactt cccataaaga taagtaaacc actagtaata atcgtaggtg ataaaatcgg
    17641 tacagcgatt ttcgtaaaaa tcacccactc attggcacca tcgattctgg cagcctccat
    17701 cagtgctgta ggaaattgac tgctatattg tcgcatcata aacactgaag ttgtactggc
    17761 aatcgaaggg atgatcacac ctgcataagt attcgataat cctaaacgac tcataatgta
    17821 aaataaagga atcaatgtgg taaaaaaagg caccatccta gatagcataa ctgtattgaa
    17881 caacagttta ttccccataa aacgatactt tgctaatgca taacccgcca ttgtagaact
    17941 ggctgtagct aaaactgtac tgattacagc aataacaatc gaattccaaa taatcaaggg
    18001 ataattatat ctcgccatga tggcttgcca gttttttaat agcatatccc caaacttgaa
    18061 attgattccg ccacttaaaa tttcttgatt actttgcgtt gaagctacaa tcatatataa
    18121 atatggcatc aaaaatatta aggcaccaaa cacaataatg acgtacgtaa aaattctgct
    18181 ttgtatcttt tgtttagtca tttttatcac ctccgaattt aaattgaata gccgataata
    18241 taacagatac aaacaccatc gtccaggcaa tagcagaggc ttttccaaaa tcaaccattt
    18301 caaaacttgt tttatataaa ttgactccta cgaccaaagc tgaattttct ggtccattgg
    18361 aagtcccaaa gaatatattt gggatattga aattacccaa gccattgata gtggctaaaa
    18421 ctgtggccat taaaatgatt ggctttagca tggggatcgt taataaaaag aattgccgaa
    18481 atttcgatgc tccatctatt tcagcacttt cgtatatctc tttagaaatc ccttgaagac
    18541 cagctaaaaa taaaatagtg ttatatccta cactaagcca aatcatcgtg atgatcattg
    18601 atattcgtat ccaaaaagga tttgttaacc actgtacacc gttaattcct agcgcattca
    18661 aaaatccatt gaataaccca gtcggattga aaaaagtcat gaatacggaa gctacagcca
    18721 ccgttgatgt aatggttgga atataataga cagttctaaa aaatcctttt ccttttaact
    18781 gtgctgaatt caataaaaca gcaaagtaaa tagctaatag catcgttatt ggaattgatc
    18841 ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa caatggatct cggaagatag
    18901 ctagataatt gtcaaatcca acaaattcaa atgagtagcc aattttcata aaactaaaag
    18961 caaacgagat aatgatggga atcaaaatcg tcacaataaa gaaaataaca aaaggtgtga
    19021 ccaatccata agcacttttt ttatgagacg tatctgttgc ttttttcttg ctgttcttta
    19081 ttgtcttgct tggtaacgaa gattgcatca tcgttaagtc ctcctaatat tttttaatca
    19141 ccagttaatt ttacgccgta cttatgagca aaattttctg cttcttttag atatgaattt
    19201 tcaaaatcac ctgtttgcca atagttatat gcagcggtag tcaaataaga cgtagcatct
    19261 gaataagtgg ctggtaaaac agtatttgat gtattttcat tgattttatt caatagttca
    19321 tagtattttt gtcctcctta gtagtcaaac tttttctcag ctgcttctgt ttgataggcc
    19381 aatgagttag atgtagccac gcccagctcc agtgcgttgg ctaagcattc ttcatctgtc
    19441 agcataaatg taagaaattg ttgtgccact tgtggatttt tggcatcttt agggacataa
    19501 aaacttgatc ctcctgatac tgcacctttt cctggatttt cttttgaaaa aggaatcagt
    19561 ggagccatgc gccatttgtc agatgcatca ggaaaattca aagacatatt cgtagcatac
    19621 cagccgccag caacaaacat tgcagattct tgaaatgctt tctcagcatc ttgtgcaccg
    19681 tagaaatgta caatatctga gtcgatcatt tctttgatta actttgcagc atgtttcgct
    19741 tctttagtat tcagacggat atttccttct tcatcaagca aagattgatt ctgctgggct
    19801 agcatgttga taaacaattc ttggtcccca gattctggca aggcaattcc aaaactacca
    19861 gttttttctt ttaatgcttt tgccacctgg atatattctt cccaagaact gatttcttca
    19921 aaatctacac ctatttcagc aaattttttt ggttgataat aacaaagtgc tggggtaaaa
    19981 tcactagccc aaggaataat cgtttcatta ctcgtttgtt gacgcaaaat attcaatttt
    20041 tgatcataaa actgatcgcc atatttgtca taaaatccat attcatcagc actataaaaa
    20101 gcatcttgaa atttttccag cacacctgct gctgaagtgt ctgccatgga gataatatcc
    20161 ggcatatctt cgtgtgttac caattttggg gtgatcattt gtactgttgc tgttcccgaa
    20221 gctacattca caatttttaa gtcaatattt gctccagttt tgcggttata gatttttgca
    20281 gcattttcaa catagtttgt cagtttgccg tttgcgacat atacttctaa tgtagtggct
    20341 ttttttatca ttggatctgc gatgtttgct gttacttctt cgttagtttt tccacatgca
    20401 cttaagctga ataatagggt aatgtttagc aaagctatca ccaatttgta tctttttttc
    20461 atgtctatcc cttctttctt ttcaagttct tatataaaac gctttcactt atgtatatta
    20521 acggaattag taacacaaat atttatataa aaaaatattt tacttgtcgt tttggctttt
    20581 catttttgtt ataatagtca ttaattataa atagaaagct ttttatataa aaagagaaag
    20641 gaaagtggtt cttttgttaa caaacaacca atcctccaat cttgtcaaaa aaacactaac
    20701 ctattccata gatacccatc ttcatttatc tgctgctccc tattttcgac tactaataaa
    20761 tgaaagcgct aatttatatg ctgaatttgc acataaatct atagaactag ataatccttc
    20821 ccttttattc attgataaac agcaagaagt ccaaatcttt tctactcaaa aggaaacaca
    20881 aacttttatc attatccagt tttattttga tgagcaatta ttagcacata ttacgggaca
    20941 atctttttct atagatcaag aaataacatc tatcattact tttctgaaaa atgaacagtt
    21001 caaaattgat tatctaaaac atagtaagcc agctatttcc tatgatcagg aaatcaattc
    21061 ttttttcata ctatgccaac agatgagcat tcatttgctt tccttattag ttacttattg
    21121 tctgcaaaaa attcaattgt cttccttacc aaggcattat caacaggaac ttgctttatt
    21181 gacaatacct tcaaatgaga aagtcattcc taaaaaaaag catattcttt attcagatga
    21241 attagttgat ccaatcatac gatatttaga agaacatctt tcagaaaatt ttttgcttga
    21301 acaattagcc caaatttttt ttatcagtcc agccagctta aaaaaacatt ttagaaaagt
    21361 gaatggaaaa agtgttatta gctattttaa agaactaaaa atggaaaaag caaaacgtct
    21421 cgtgctagaa aatgaattaa cctatacaga aattgcagat atattaggat ttcattcgat
    21481 ccatcatttt tcttcagctt ttaaaaaata tacagggtta tccccttcaa agtattatct
    21541 atccttaaaa tagaaaaaaa tttttaggtt ttatccacaa attatttctc tatattttct
    21601 gtctattcaa aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca
    21661 tagtacgatc aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta
    21721 gctggcggtc cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt
    21781 atctatgaac gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga
    21841 ggtgtgatca ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa
    21901 aacaacatcg atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga
    21961 ttaacagtta aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc
    22021 atctcttctg gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac
    22081 ggctttgtcc tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa
    22141 aatccacaac atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat
    22201 ttcaagcatc gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct
    22261 tatgatccag aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt
    22321 catttagaaa caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat
    22381 ggctccgtcc tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa
    22441 gtggtgaaag caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag
    22501 ttacaaacca atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta
    22561 gtgactggtc agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg
    22621 ctggcagata ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc
    22681 gcaaaaatct ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca
    22741 tcaggcataa caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat
    22801 ccagaagcta caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta
    22861 ggtggacaag catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca
    22921 atcaccggaa acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg
    22981 tattctacta cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg
    23041 aatatcgtag aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc
    23101 tttttagata tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat
    23161 attccaatga atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac
    23221 attactttca gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt
    23281 atcaaagcga ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg
    23341 agttattttt acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta
    23401 tatagaatag aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta
    23461 tagtttaaga aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa
    23521 aaaacatgtt gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac
    23581 gcctaatatt acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct
    23641 ttcgaatatt ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg
    23701 actggcttgc gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa
    23761 tcatattttt cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg
    23821 tattaaataa tttaattttt ttgttcctat ttatttaaca
//
LOCUS       DACEMK010000042        23646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEMK010000042 DACEMK010000000
VERSION     DACEMK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.042"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            248..523
                     /locus_tag="IW715_001418"
     CDS             248..523
                     /locus_tag="IW715_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022027.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            536..2614
                     /locus_tag="IW715_001419"
     CDS             536..2614
                     /locus_tag="IW715_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1022028.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2774..4660
                     /locus_tag="IW715_001420"
     CDS             2774..4660
                     /locus_tag="IW715_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1022029.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4672..5619
                     /locus_tag="IW715_001421"
     CDS             4672..5619
                     /locus_tag="IW715_001421"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1022030.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5639..6166
                     /locus_tag="IW715_001422"
     CDS             5639..6166
                     /locus_tag="IW715_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1022031.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6229..6882)
                     /locus_tag="IW715_001423"
     CDS             complement(6229..6882)
                     /locus_tag="IW715_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1022032.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7014..7856
                     /locus_tag="IW715_001424"
     CDS             7014..7856
                     /locus_tag="IW715_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1022033.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8014..8910
                     /locus_tag="IW715_001425"
     CDS             8014..8910
                     /locus_tag="IW715_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1022034.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8913..9626
                     /locus_tag="IW715_001426"
     CDS             8913..9626
                     /locus_tag="IW715_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1022035.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9646..10164
                     /gene="msrA"
                     /locus_tag="IW715_001427"
     CDS             9646..10164
                     /gene="msrA"
                     /locus_tag="IW715_001427"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1022036.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10161..10388
                     /locus_tag="IW715_001428"
     CDS             10161..10388
                     /locus_tag="IW715_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1022037.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10540..11091
                     /gene="lepB"
                     /locus_tag="IW715_001429"
     CDS             10540..11091
                     /gene="lepB"
                     /locus_tag="IW715_001429"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1022038.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11174..11716)
                     /locus_tag="IW715_001430"
     CDS             complement(11174..11716)
                     /locus_tag="IW715_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1022039.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11953..12351)
                     /locus_tag="IW715_001431"
     CDS             complement(11953..12351)
                     /locus_tag="IW715_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1022040.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12611..13471
                     /gene="ylqF"
                     /locus_tag="IW715_001432"
     CDS             12611..13471
                     /gene="ylqF"
                     /locus_tag="IW715_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1022041.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13464..14231
                     /locus_tag="IW715_001433"
     CDS             13464..14231
                     /locus_tag="IW715_001433"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1022042.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14287..15144
                     /gene="dprA"
                     /locus_tag="IW715_001434"
     CDS             14287..15144
                     /gene="dprA"
                     /locus_tag="IW715_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1022043.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15266..17344
                     /gene="topA"
                     /locus_tag="IW715_001435"
     CDS             15266..17344
                     /gene="topA"
                     /locus_tag="IW715_001435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1022044.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17313..18689
                     /gene="trmFO"
                     /locus_tag="IW715_001436"
     CDS             17313..18689
                     /gene="trmFO"
                     /locus_tag="IW715_001436"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1022045.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18887..19792
                     /gene="xerC"
                     /locus_tag="IW715_001437"
     CDS             18887..19792
                     /gene="xerC"
                     /locus_tag="IW715_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1022046.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19829..20377
                     /gene="hslV"
                     /locus_tag="IW715_001438"
     CDS             19829..20377
                     /gene="hslV"
                     /locus_tag="IW715_001438"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1022047.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20391..21791
                     /gene="hslU"
                     /locus_tag="IW715_001439"
     CDS             20391..21791
                     /gene="hslU"
                     /locus_tag="IW715_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1022048.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21809..22600
                     /gene="codY"
                     /locus_tag="IW715_001440"
     CDS             21809..22600
                     /gene="codY"
                     /locus_tag="IW715_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1022049.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22682..23557
                     /locus_tag="IW715_001441"
     CDS             22682..23557
                     /locus_tag="IW715_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1022050.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8191 a         4078 c         4981 g         6396 t
ORIGIN      
        1 cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga aagtaaacat
       61 ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata ctatttggaa
      121 tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg atgaaacaat
      181 gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg tataggagat
      241 ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc gctatggcgc
      301 cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa atatattgat
      361 gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa aacgtgtaca
      421 gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa ttttaaagaa
      481 aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat ggacgatgga
      541 atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca tagctagccg
      601 cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg tattaggtct
      661 gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta tgattattgc
      721 accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca aaaatttcgg
      781 gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg taggtgcgac
      841 gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg gagcagcgtt
      901 aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata tccctaaaaa
      961 ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg ttactgcctt
     1021 ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg cggggatgag
     1081 tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct ggttcaaaca
     1141 aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt atctgctgat
     1201 cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg tatctggaat
     1261 cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa tctctgtgtt
     1321 tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt ttacactgaa
     1381 tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc cagtatgggg
     1441 agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct ctgtcatgct
     1501 ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg gcagtaaaaa
     1561 gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa agggaacagt
     1621 cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt tttaccagcg
     1681 gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca tagggatcat
     1741 tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga atgctttgat
     1801 gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg atcgtaatac
     1861 aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga tacgtgatct
     1921 gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac aagcactgat
     1981 tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc tttcttctaa
     2041 tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca caagccagat
     2101 tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca
     2161 tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta gtgatgaaga
     2221 tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg aaagtttaga
     2281 taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc aacgcaaact
     2341 tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc agcaagatag
     2401 tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca ttgatgaata
     2461 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
     2521 gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
     2581 agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc aagttttata
     2641 aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg agaacacaaa
     2701 aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac aatatccaag
     2761 ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa cttatggtga
     2821 aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta tcgggttgat
     2881 tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta tcgatagcag
     2941 cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt atctttctca
     3001 agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag gaaacagccc
     3061 cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg aaaacggaga
     3121 agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata aagaagatgc
     3181 ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca gtgaattaga
     3241 taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt
     3301 gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag attatgaagc
     3361 gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga tggttaccca
     3421 cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt
     3481 atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt
     3541 agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag cttggatgag
     3601 ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct
     3661 aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc tagcgacaca
     3721 acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa atgaccaaac
     3781 actgctagaa catttagatt tattgattca atctcgggaa agactaggta ttactggtaa
     3841 aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc ccctggatag
     3901 cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag aaaatgaaga
     3961 aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag aagcaaaaac
     4021 agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat ttccaagatt
     4081 tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt tatttttgtt
     4141 gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca atgatttgga
     4201 catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg ctgtgatcac
     4261 tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag tattccaagg
     4321 aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga agcaaaaaga
     4381 gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa
     4441 gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg agattgcaga
     4501 acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag ataatttcac
     4561 tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat
     4621 ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag gatggaacaa
     4681 gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca
     4741 ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt
     4801 cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt
     4861 ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa
     4921 tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt
     4981 ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt
     5041 ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac
     5101 atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc
     5161 aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca
     5221 tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt
     5281 tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta
     5341 ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca
     5401 ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat
     5461 atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg
     5521 aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat
     5581 gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat
     5641 gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc
     5701 ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt
     5761 gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat
     5821 tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga
     5881 agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc
     5941 tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt
     6001 attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac
     6061 tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg
     6121 taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata
     6181 gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt aagtaaagaa
     6241 taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc aaacaacatg
     6301 catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc
     6361 tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa cagcagccac
     6421 gacacagagc caccccataa aaatatagat gatcgtagat acttttgata ctctctcccg
     6481 tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc agattaatac
     6541 aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag tataacttcc
     6601 tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt ttgcttttgt
     6661 aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca tcgatccata
     6721 gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta ctaaaagaac
     6781 cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat taaacacttc
     6841 atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg ctcctttact
     6901 aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa attgtcttac
     6961 atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa ataatgacaa
     7021 atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc gatgctttag
     7081 gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc gatgatgacc
     7141 aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa
     7201 gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa gacggcagtg
     7261 aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca gcaagacaag
     7321 ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact gaccaaggat
     7381 tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa
     7441 tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga atatccacac
     7501 tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg tctagtgttt
     7561 tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta actaaggggc
     7621 gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg aaagaattgc
     7681 caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg caagaattca
     7741 aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat acgaatccgg
     7801 ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta gattagtcga
     7861 aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc tttataaaat
     7921 taggaaaata attgttttct tttttcataa agactacctt tttgataaaa tttttgataa
     7981 cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg acatttgatc
     8041 ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc tgcttctata
     8101 ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc aaataaaaaa
     8161 gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg agatcaaacc
     8221 aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta taatttgacc
     8281 gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat cttaaagcga
     8341 gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat cacactgact
     8401 gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt aacggtcaat
     8461 tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt ggaagaattt
     8521 cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc attttatttg
     8581 aactttcctg agatcactga catgcagaca attgttaaca attggaacaa aggaacagaa
     8641 acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt gctttatcaa
     8701 ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac tagcacgaca
     8761 gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt ccatccaaat
     8821 aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga gacacaaaac
     8881 caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac gtatcttcaa
     8941 acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt ggggtagtac
     9001 ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca gaactcccag
     9061 aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat tcaaataaag
     9121 aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat agtgatattg
     9181 aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa gtactaggtt
     9241 ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga aatgtccaac
     9301 taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat gtgatgaata
     9361 tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat gatctgacta
     9421 tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa gcagataaaa
     9481 tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat acctctgatg
     9541 cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc aagacaaaag
     9601 aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga acgagcaata
     9661 tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc tggtatccat
     9721 acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga acaagtaaaa
     9781 agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac attgatttca
     9841 tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc tttcggacaa
     9901 ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga agaacagcgg
     9961 atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga acagccaatc
    10021 gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca tcaagagtat
    10081 tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc ctttatcgaa
    10141 gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg acactgcgag
    10201 gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa gacatccaat
    10261 ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg aatgtcgact
    10321 atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa aataatcatg
    10381 acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca atagcctaat
    10441 tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt ttcttataat
    10501 aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa acaacgctat
    10561 attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct tgcgttcatg
    10621 ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga aaacgtgttg
    10681 aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt tgatattgtt
    10741 gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct tccaggagaa
    10801 aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa ggaaccttat
    10861 ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga ctttacacta
    10921 caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact tggagataat
    10981 cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga tattctaggt
    11041 aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg agaaacaata
    11101 aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca gtttactacc
    11161 ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa gttttgataa
    11221 atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg aatctaccta
    11281 gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa ttgataaaaa
    11341 ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg tttatctttt
    11401 tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata agaaaagcag
    11461 cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac atttcaagca
    11521 aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa aatccgaatg
    11581 tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca agggctaata
    11641 aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa acgtcttttg
    11701 caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg ataccataaa
    11761 atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa caattaaaaa
    11821 aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat aaaaaaaata
    11881 gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa aaacgtcaga
    11941 ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt ctagatcttg
    12001 gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt ggaaagtacc
    12061 accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt caacactaaa
    12121 cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt ttataaattt
    12181 tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata ccattgaacc
    12241 agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat aaaaagcaac
    12301 ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca tttgtttcgt
    12361 ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca aaaatttaga
    12421 ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga tgtgatagaa
    12481 tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat aaaaaagtga
    12541 atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta tgaaaggaat
    12601 ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc gacgagaagt
    12661 ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac gattaccttt
    12721 atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc ttgttttatt
    12781 gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat attttcgtaa
    12841 acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta aaggtctagt
    12901 acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc gggggatcaa
    12961 gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat ctacgttgat
    13021 gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg taacaaaagg
    13081 tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag ggattttatg
    13141 gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg cgatcaaaga
    13201 ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg cccgatatta
    13261 ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa aattggcacc
    13321 ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg atcgtggcag
    13381 cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac tggatcgttg
    13441 cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa gaacgattga
    13501 aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat ccacgcaaag
    13561 gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca ttaattaagg
    13621 aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt caccaatgga
    13681 ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg gcagctgcag
    13741 ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa ttatcagaga
    13801 aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc gggatcggga
    13861 tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa ttagcgatga
    13921 tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat gcgatgacct
    13981 tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct gtgtcgattg
    14041 ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa tatgacgtgc
    14101 tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa cacttagaag
    14161 gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct gtaaaaaaat
    14221 atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac gaaaggcggt
    14281 tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa aggaatcagt
    14341 agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa agaaatcgga
    14401 tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt tcgcaaaagt
    14461 tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt tatcacttgc
    14521 tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat tgtactgttt
    14581 tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg tggaagacac
    14641 acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat agaagctgga
    14701 tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca agcagcaatc
    14761 tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt ctatcctgct
    14821 gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag tgaatatcct
    14881 cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat cgcagctttg
    14941 tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat aacagcacag
    15001 caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt aacagaacat
    15061 tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc gggtgaagat
    15121 atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt gacaaacgtt
    15181 ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag taggaacacg
    15241 acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg tagaatcacc
    15301 tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa tggccagtgt
    15361 tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa acaattatga
    15421 accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga aagccgcagc
    15481 aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag aagcgattgc
    15541 ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg tggtcttcaa
    15601 tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga tcgatgtaga
    15661 tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt attcgatcag
    15721 tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc aatctgtcgc
    15781 tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag aagaatattg
    15841 gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt tctggggaat
    15901 cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca ctgctagaat
    15961 tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa gaaaccctgc
    16021 gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa actttcgaac
    16081 aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg gtaaacaagg
    16141 aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg atacagcaaa
    16201 agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta gacatgatca
    16261 ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc gtccaacgag
    16321 tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat tgaaacttta
    16381 tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt tagatacaat
    16441 gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa aaatcaaatt
    16501 caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag agaaagaaaa
    16561 tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg aaccaaaaca
    16621 acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg cactagaaga
    16681 aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc aaagacgcta
    16741 ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa ttgtcaattc
    16801 attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag aaatggaagg
    16861 agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg atcgtttcta
    16921 taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa ttcaaatcaa
    16981 agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga taaaattagg
    17041 gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata cgaaaccaat
    17101 cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca ttgaacgaaa
    17161 atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg aattcacttc
    17221 ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg tcgagaagaa
    17281 agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag acatccaaaa
    17341 ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca gggcttgccg
    17401 gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta tatgaaatgc
    17461 gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta gtatgttcca
    17521 attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa gaaatgcgcc
    17581 gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct ggtggtgcct
    17641 tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa gagcatccat
    17701 tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc gtagtagcaa
    17761 caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat ggttcagatg
    17821 gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc gatatggata
    17881 aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac tgtccgatga
    17941 cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg gcagaactta
    18001 aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta atggcacagc
    18061 gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa gatcctaaaa
    18121 ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca gcttctttat
    18181 acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt gttttccgga
    18241 tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac cgaaacagct
    18301 ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc gaagatctgt
    18361 tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg agcggactac
    18421 ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag tttccacaag
    18481 aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa catttccaac
    18541 cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga gacaaaaaag
    18601 caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata aaaacagaat
    18661 tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg agacggatag
    18721 tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg aggaattgcg
    18781 ccaattgtga acaaacttag aaatattggt taaattctga caatttattt gtctttttat
    18841 aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga cagaagccac
    18901 gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag aaaaaacgaa
    18961 agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat ctggagacgc
    19021 tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat tagctgatca
    19081 tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac gttcgtttta
    19141 tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg ttcatctcaa
    19201 aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag cactttttga
    19261 cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg aagtccttta
    19321 cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca ttgattttga
    19381 ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc ctctaggttc
    19441 ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat tgatgacaaa
    19501 atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa tcacgcctac
    19561 tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata gtgatatcca
    19621 tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg cagatttgcg
    19681 gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct atgcccatgt
    19741 aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt aaaaataagg
    19801 aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt caaccactat
    19861 ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag taacaatggg
    19921 tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca atggagaagt
    19981 tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa aatttgaagg
    20041 caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag ctcaagaatg
    20101 gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga atgataaaga
    20161 aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta tcttagctat
    20221 cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact atgctcaaaa
    20281 cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag atatctgtat
    20341 ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat atgtatgaat
    20401 taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta ggacagcacg
    20461 cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg cagttagatg
    20521 aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct acaggggtcg
    20581 gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt gtcaaagtcg
    20641 aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca atggttcggg
    20701 atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt gtatacacaa
    20761 gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt atcaaaaaag
    20821 aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat gctagtcagc
    20881 aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat cgccgtacag
    20941 tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt gaagttgaag
    21001 accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg atcgacctaa
    21061 atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta acagtcaaag
    21121 aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat gctgatatcc
    21181 atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat gaaatcgata
    21241 agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc caaagagaca
    21301 tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc caaacagatc
    21361 acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat ctaattccag
    21421 aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct gaagattttg
    21481 tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg ttgatcggta
    21541 cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct caaatcgctt
    21601 tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc attttggaaa
    21661 aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa ataacgatca
    21721 cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat ttaagtcgct
    21781 atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa cccgcaagat
    21841 aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc cttacgataa
    21901 aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag gaagtgacgg
    21961 agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca aaacaatgtt
    22021 tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga tgaaaactga
    22081 agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa gagagaaata
    22141 tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt tgggtacgat
    22201 tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag cagaatatgg
    22261 agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg aagcagatgt
    22321 acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg aattgaaggc
    22381 tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg cttctaatat
    22441 tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa aattggaatc
    22501 agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa aagtattgaa
    22561 ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg actggggcag
    22621 atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg gaggaatggc
    22681 aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac acggtgcgga
    22741 attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag gtgatccagc
    22801 gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa aagatgatca
    22861 gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc gggatatgga
    22921 ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga gtacgccgga
    22981 gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat taggaggaga
    23041 cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt tcttttctat
    23101 cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg aagatttcta
    23161 tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc cattcatcga
    23221 tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc gtgaactttt
    23281 tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca ttcgaagtga
    23341 aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag ggatctggtc
    23401 tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa tcgctgacac
    23461 ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag gtgcccatga
    23521 aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg agaagttagc
    23581 agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata agggagtgtg
    23641 acatcg
//
LOCUS       DACEMK010000043        23582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEMK010000043 DACEMK010000000
VERSION     DACEMK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.043"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW715_001442"
     CDS             <1..1152
                     /locus_tag="IW715_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022051.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW715_001443"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW715_001443"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1022052.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW715_001444"
     CDS             2728..3003
                     /locus_tag="IW715_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1022053.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW715_001445"
     CDS             3009..3776
                     /locus_tag="IW715_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1022054.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW715_001446"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW715_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1022055.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW715_001447"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW715_001447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1022056.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW715_001448"
     CDS             7920..8222
                     /locus_tag="IW715_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1022057.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW715_001449"
     CDS             8225..9388
                     /locus_tag="IW715_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1022058.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW715_001450"
     CDS             9414..12842
                     /locus_tag="IW715_001450"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1022059.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW715_001451"
     CDS             12863..13987
                     /locus_tag="IW715_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022060.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW715_001452"
     CDS             13977..14414
                     /locus_tag="IW715_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1022061.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW715_001453"
     CDS             14398..14736
                     /locus_tag="IW715_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1022062.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW715_001454"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW715_001454"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1022063.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW715_001455"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW715_001455"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1022064.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW715_001456"
     CDS             15893..16936
                     /locus_tag="IW715_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1022065.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW715_001457"
     CDS             17095..17823
                     /locus_tag="IW715_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1022066.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW715_001458"
     CDS             17939..18184
                     /locus_tag="IW715_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1022067.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW715_001459"
     CDS             18181..18399
                     /locus_tag="IW715_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1022068.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW715_001460"
     CDS             18535..19074
                     /locus_tag="IW715_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1022069.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW715_001461"
     CDS             19067..21340
                     /locus_tag="IW715_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1022070.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW715_001462"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW715_001462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1022071.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW715_001463"
     CDS             complement(22513..22767)
                     /locus_tag="IW715_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1022072.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW715_001464"
     CDS             complement(23167..23436)
                     /locus_tag="IW715_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022073.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         7878 a         4026 c         5073 g         6605 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aa
//
LOCUS       DACEMK010000044        23268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEMK010000044 DACEMK010000000
VERSION     DACEMK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.044"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            452..1825
                     /gene="glmU"
                     /locus_tag="IW715_001465"
     CDS             452..1825
                     /gene="glmU"
                     /locus_tag="IW715_001465"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1022074.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1919..2890
                     /locus_tag="IW715_001466"
     CDS             1919..2890
                     /locus_tag="IW715_001466"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1022075.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2896..2998
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3064..3633
                     /gene="thiT"
                     /locus_tag="IW715_001467"
     CDS             3064..3633
                     /gene="thiT"
                     /locus_tag="IW715_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1022076.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3714..4487)
                     /locus_tag="IW715_001468"
     CDS             complement(3714..4487)
                     /locus_tag="IW715_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022077.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            4753..6453
                     /locus_tag="IW715_001469"
     CDS             4753..6453
                     /locus_tag="IW715_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1022078.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            6423..6641
                     /locus_tag="IW715_001470"
     CDS             6423..6641
                     /locus_tag="IW715_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022079.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            6661..7551
                     /locus_tag="IW715_001471"
     CDS             6661..7551
                     /locus_tag="IW715_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1022080.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            7569..7853
                     /locus_tag="IW715_001472"
     CDS             7569..7853
                     /locus_tag="IW715_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022081.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            8340..8954
                     /gene="gmk"
                     /locus_tag="IW715_001473"
     CDS             8340..8954
                     /gene="gmk"
                     /locus_tag="IW715_001473"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1022082.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            8959..9264
                     /locus_tag="IW715_001474"
     CDS             8959..9264
                     /locus_tag="IW715_001474"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1022083.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            9517..11925
                     /gene="priA"
                     /locus_tag="IW715_001475"
     CDS             9517..11925
                     /gene="priA"
                     /locus_tag="IW715_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1022084.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            11940..12431
                     /gene="def"
                     /locus_tag="IW715_001476"
     CDS             11940..12431
                     /gene="def"
                     /locus_tag="IW715_001476"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1022085.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            12424..13362
                     /locus_tag="IW715_001477"
     CDS             12424..13362
                     /locus_tag="IW715_001477"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1022086.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            13362..14720
                     /gene="rsmB"
                     /locus_tag="IW715_001478"
     CDS             13362..14720
                     /gene="rsmB"
                     /locus_tag="IW715_001478"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1022087.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            14733..15473
                     /locus_tag="IW715_001479"
     CDS             14733..15473
                     /locus_tag="IW715_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1022088.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            15470..17539
                     /gene="pknB"
                     /locus_tag="IW715_001480"
     CDS             15470..17539
                     /gene="pknB"
                     /locus_tag="IW715_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1022089.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            17704..18603
                     /gene="rsgA"
                     /locus_tag="IW715_001481"
     CDS             17704..18603
                     /gene="rsgA"
                     /locus_tag="IW715_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1022090.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            18616..19266
                     /locus_tag="IW715_001482"
     CDS             18616..19266
                     /locus_tag="IW715_001482"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1022091.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            19267..19905
                     /locus_tag="IW715_001483"
     CDS             19267..19905
                     /locus_tag="IW715_001483"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1022092.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20060..20914
                     /gene="mreC"
                     /locus_tag="IW715_001484"
     CDS             20060..20914
                     /gene="mreC"
                     /locus_tag="IW715_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1022093.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            20918..21427
                     /gene="mreD"
                     /locus_tag="IW715_001485"
     CDS             20918..21427
                     /gene="mreD"
                     /locus_tag="IW715_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1022094.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            21682..23220
                     /locus_tag="IW715_001486"
     CDS             21682..23220
                     /locus_tag="IW715_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022095.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT         7822 a         4238 c         4994 g         6214 t
ORIGIN      
        1 aatggctttc cacatttttt cataaatttg gcttaatacc gagaaactgg cttttgaaca
       61 ccgtttatct agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga
      121 aaaattgctc ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa
      181 aacgtttaat gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc
      241 aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag
      301 agcaccggtt accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac
      361 gctgggataa aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga
      421 aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc
      481 ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg
      541 taaacctatg gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat
      601 tacgatcgtt ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata
      661 tgcgttacaa gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt
      721 ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga
      781 aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac
      841 ggcacaagca gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt
      901 agaaaaaatc gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa
      961 tacaggaact tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac
     1021 aaacaatgct caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg
     1081 ccatacagtt gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg
     1141 gatcgcgtta gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt
     1201 caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg
     1261 tccagacaca ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga
     1321 ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat
     1381 tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc
     1441 acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt
     1501 gaaaaatgct caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc
     1561 tacgctggga gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa
     1621 gaacaaacat catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat
     1681 cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa
     1741 tattccagaa tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc
     1801 aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat
     1861 atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat
     1921 gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt
     1981 agcagagaaa attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt
     2041 tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt
     2101 tattcaatca acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga
     2161 cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc
     2221 gcgtcaagac cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat
     2281 gattcaaaaa gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca
     2341 aggtttcttt gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt
     2401 cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt
     2461 cacacgggca cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg
     2521 ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac
     2581 ttgtgtactg atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc
     2641 tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg
     2701 ccctgcttta caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat
     2761 ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat
     2821 gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa
     2881 aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt
     2941 ctaaaccctt cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt
     3001 tgactatttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt
     3061 tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg
     3121 ttgtcgttga ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca
     3181 ttgactcttt ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg
     3241 ggcatcctac attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt
     3301 gagtacccga ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa
     3361 aaaggctatg tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg
     3421 attggcgcac tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg
     3481 tatgcactat gggggatgaa ttcttggctc ttttctttcg taatgaatgg tgccagcggg
     3541 ttgagtacag ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg
     3601 atttttgtgc cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac
     3661 agaatcatgt cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc
     3721 aatggcagat aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag
     3781 tagattaaag tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata
     3841 taatcttcaa tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca
     3901 aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg
     3961 attttttcaa caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac
     4021 aagatcgaac ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa
     4081 atccgattca gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa
     4141 aacggcgaat gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa
     4201 ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat
     4261 ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact
     4321 ttcagccctg tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag
     4381 ttttgaggaa aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa
     4441 acagcattac ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc
     4501 ttatttcctc ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt
     4561 attttaaaat tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat
     4621 ttttgtgatt tttgtatttt ttatttaaag gatgttttat gaattgtttt cttatggaat
     4681 ctacaaagtc gaatacgaga ctcatgacct ttttatgacg tactggtata cttgaagaaa
     4741 aaggagagcg cgatggagat aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg
     4801 cctatccagc aagcaaaaca accccttcca ttttttcaaa gtcatcaaga aagtagagac
     4861 gctttttggc gttctgcaaa aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc
     4921 gtctatttta ctattgctaa aagtagagga gatgttattt ttaataatct gttgattgaa
     4981 ggtgaaactt ttgaatggaa aaaatttttt cggtatatgg aagcttgcgc tcgttttttt
     5041 attaaagaaa actgttgttt tttatttcaa tctcctctat cagaagaatg gttgcgtatt
     5101 tttcataaaa acggctatca aggaaccaca caattaaata aaaagctggt ctatcataca
     5161 gcacttgttt taggtggagg aggagcacac ggtgcttatc agattggtgt gtggcaggcg
     5221 ctgaaggaac atgatatcaa ctttgagatt atcacaggta cctcggtagg tgcgttgaac
     5281 ggcgcattga ttttacaagg agatatgaaa aaagctgtga atttatggaa aaagctgtcg
     5341 acaagacaag tgcttgcgtt accagaaatg gctgcagttg aagaccttcg tgaacgcttc
     5401 tatcgagaaa gacgccaaat gacgaaaaca gcactaattg aaggcggggt ttcttctgct
     5461 ccattggaaa agatggtcaa agacaatttg gatttgtcct tactcaaaca taatcccaaa
     5521 atacgtttat atacggtgtc tactaaactg ccagaactag cggaagtagt gactgatatc
     5581 caacagacaa aaacagaaga aataccagat tggatactcg cctctgcttc attttatccc
     5641 gctatggctt atcgaaagat cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa
     5701 atacctattg atattgcgat taacaaagga gcaacagagg catttgtcgt agatgtgcaa
     5761 gggccaggcc cagcgaaaaa aattcgtatg ccagacattt ttatccattg gaaatgtcaa
     5821 acattgtgga cactaggcag ctttctgctt tttgatagcc aaaggaacca attaaattta
     5881 caattaggct atctggaaat gaaaaaacga ttaggctatt actatgggaa ctggtataca
     5941 tttcattctg ttaaacctgc aagaacatac tggcgaggat ttttgtctta cttaacaaaa
     6001 gaaattcagt tagaattatc ttttttcaaa agcataaaat tttggcaaaa actgcgtaat
     6061 ttatataaga acagggtcgt accagagaca tgcggattgg cgatgctaga acttttagca
     6121 aaaaaacatt ttttactgcc caatgaaatc tatcaagtag acatgatgat tcaaatgatc
     6181 tgccaacaag atataaaaag tatgccggat gatttattgg cacaaattgg tcaattaagt
     6241 actgaagaat ggcgaaggta tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa
     6301 actgtttatt ttgattatct gctacaagga aaacgcaagg atcgtttgca gcatgaattg
     6361 cttaagcaac cagttgacac attactgatc ctatatttat attatctcaa ggaggaacaa
     6421 ccatggcaca agaattttca tatgaaatcg tagaagaaat cgctatattg tcagaaaaca
     6481 acaaggggtg gcgtaaagaa ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg
     6541 atcttcgaga ttgggcgcca gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg
     6601 aagagtttga acaactgcaa aaagcaatag aaaatatgta atgaggaagg accctgtcga
     6661 atgaaaagta tgactggttt tggcaaagcc atccgcgaaa caaaagcgta tcagttagat
     6721 attgaaataa aaagtgtcaa ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc
     6781 ctgaactttg ttgaaaacga ttttcgtcag cttattaaac agcagttaag ccgcggtcga
     6841 atcgaagtat ttgtgaatct agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac
     6901 tgggaattga ttgattcact tatcactcaa ttgacagacg gaattcaaca acgatacggt
     6961 agtcaaaatc aagtgtcaat tagtcggatg gcggaacgct tcgcgctgga cgaaaacttc
     7021 gtaacggtag aagaaaaacc ggaagagagc atggaagaat tggcacagtt agctctagat
     7081 acattgaaaa gcgcacttga ttctattgag gcgagtcgag aaaaagaagg tcaagcttta
     7141 gctttagtgt tgaaaaagaa tactgaagaa ttcaaagaag tactaaaaaa actcgatagt
     7201 tttgtagaaa tctatgaaaa ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt
     7261 cttggagcga aagtcgatga gcaacggttg ttgacggagc tagccatctt gttggaacgc
     7321 ggagatattc acgaggaact ggatcgtctg gcgatccatg tacaaaaact agatgaactt
     7381 atcgaagcac aatgtcctgt aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg
     7441 gaaatcaaca cgatcggttc caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag
     7501 tctaagacga tcttagaaaa aatcagagaa caagtacaaa atattgaatg acgaaagagg
     7561 tagagataat gaaatattat gacgtaacgt tccacgaatt aagtggaaaa actgtggtta
     7621 aacgggatat cccttctgaa aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact
     7681 ataatgagaa cgaactgttt attttgatca acgacggtgc ttacgtgacg atgaatcgga
     7741 aatttattgt tcgaattgac acagaagagg tagaagatcc gacagaaaaa gcacgttctc
     7801 gaaaagatga gatcatgggt gtagtgaata ctttgtccaa tatgggcttt tagcaaggat
     7861 gctgaacaaa gaaatacaat aaataatcag aattggattt cttcgcttag cttctttgct
     7921 ctaggaatac atttaaaggt tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat
     7981 aagtgatgtt caaaaaccat tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa
     8041 actattttca caaaattttt cttcatactc gaaactaatc ggcttcttca cgacctctta
     8101 tacacggcgt tcaaaagctt gcatctgcga taataacccc aaccaaacga aaaacatgag
     8161 aagctgttta tgtttggctg ttcttattac ttgatgcagg acagcttttt tacgacctct
     8221 tggtacaggg tagctttttt accaatttct agctataatt aaagataacc ttgaaagttg
     8281 cagaaaaaaa gtgcaaaata aacgataagt acaaaaagac tagttgaaag gacgtcgtca
     8341 tgtcagagcg tggattatta atcgtgttgt ctggtccatc cggtgtagga aaaggcacag
     8401 tacgaaaagc aatttttgag agtgaggata atgattttca gtattcgatc tcaatgacta
     8461 cccgtaaaat gcgtgaaggg gaggtcgaag gcgttgatta tttcttccga agcaaagaag
     8521 agtttgaatc aatgatcgaa gctggagaga tgctggaata cgcagaatat gtcgggaatt
     8581 attatggaac tcctttgtca tatgtcaaca agacattgga tgaaggaaaa gatgttttct
     8641 tggagattga agtccaagga gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca
     8701 tctttttgac tccgccagat ttagcggaat taaaatcacg gatcgtaggc cgaggaacag
     8761 atgcagatga agtaatcgaa gaacgaatga aagttgcaaa agaagaaatc gaaatgatgg
     8821 ctctttacga ctatgctgtt gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa
     8881 acattattgc cagtgaacat tttcgagtag atcgcgtaat cggtaaatat attaaaatgt
     8941 taaaggagat gtagtcttat gatgttaaaa ccttcaattg actcactatt agatcgtgta
     9001 aattcaaaat attctctagt gattttagca agtaaacgcg cgcatgaatt ggatgcaagt
     9061 gcacagccta ctctagattc ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa
     9121 atcgatgcag gaaatgtagt caatgatcca catcctgaat taaaacgtga acgtttgaag
     9181 atggaagaag aggaacgtca agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt
     9241 atccgtgaag agcaaaaaat gtaataaaga aaaatagagc gaacatctaa aaagaaaagc
     9301 aaacccagct gtttcctttt caaacattcg ccacttattg tctggtagaa aaggctgtgt
     9361 ctgaactttt tgacacagtt cttttttttc ttatctgctg ttaatttttt gttaggattt
     9421 ggcagaagat aagacaaatc catcgatctc tagtaaaata ggcaaggaac tttttgtcgc
     9481 ttgataagaa aagacaaaaa aaggaggaaa cgaaccatga cattaacagc tgaagtcatt
     9541 gtggacgtac ctactatgca gaccgatcag ccattcactt atttggttcc ctctgaaatg
     9601 gaaacagctt tgcaagtcgg gatgcgagta gaagtccctt ttggtaatgg caatcgtcat
     9661 gtccaaggat ttgttatggc tatccaaaaa tcagaagaat cagctaatcc atctttgaag
     9721 gcagttattc gtttgctaga ccttgctccg gtggtcaatg aagaattact ttcattagct
     9781 gattatatga aaaaaattac ttacgcattc aaaatcactt gtctacaaac gatgcttccg
     9841 agtgtgatga aagcagaata tgataaattg atctatcctt tggcagacac accagaagtt
     9901 caggtattgt ttgatcagcg agaagtgatt tcctggaagg aagcagaaga agaaggcagc
     9961 ctttctcaat tgattcgctg gcgccaagag caattagtag atatcaaata tgaagtccat
    10021 acacgaaata aagtaaaaac gattcgtttg gtccgttctt tgttaactga aaagcaaatc
    10081 gaagaagaat gggcaaaatt acggcaaaat gccaagaaac aaaaagaact tcttttgtgt
    10141 ttatctgaac tgagccaaga agaaccaatt gcttatttca aaaataaagg aatcagtact
    10201 gctgtcttga atcaaggaaa agaaaagggc tggctggagt ttatagaatc agaacgttat
    10261 cgcgaccctt ataaagaccg agtatttgac caaacgacgg cattggaact gaatgcagaa
    10321 caaaaaaatg ctgtcgaaca aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt
    10381 ttgctggagg gaatcactgg cagcgggaaa acagaagtat atcttcaggc aatcgctgat
    10441 gttttgtcag aaaataaaac ggctattatg ttagtgcctg aaatcgcatt gactccgcaa
    10501 atggtcgagc gattcaaagg acgcttcgga gaatcagttg cggtcttaca tagcggactt
    10561 tcccaaggtg aaaaatatga tgaatggcgc aagattgagc gggaagaagc gcaagtagta
    10621 gtaggcgcac gatctgctat ttttgccccg ctgaaaaata tcgggctgat cattatcgat
    10681 gaagaacatg aatcaagtta caaacaagat gagacaccgc gttatcatgc gagagatcta
    10741 gcaatttggc gcagtaaata tcatcattgc ccaatagttc taggaagcgc gacgccctct
    10801 ttagaatcaa gagcacgagc tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga
    10861 gccaaagcag ctgcacagct tccagctatt gagatcgtag atatgcgtga agagtttcag
    10921 aatcatcgga cctctacttt ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac
    10981 aaaaaagaac aaactgtcct actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt
    11041 agggactgtg gatttgttct tccatgtcct aattgcgata tctctcttac actgcatatg
    11101 gatacacgtt caatgcgctg ccattattgc ggacatgaag aacctattcc caatcgctgt
    11161 cctaattgtg gcggcaataa aatccgctac tatggaacag gtactcaaaa agtagaagag
    11221 gaactccgag aactttatcc gcaagctagg attttgcgta tggatgtaga taccactcga
    11281 agaaaagggg cacacgaaca gattttacaa aaattcggtg caaaagaagc agatatatta
    11341 ttagggacac aaatgattgc taaaggtttg gactttccag aagtgacatt agttggggtg
    11401 ttgaatgctg atacctccct gaatttaccg gattttcgtt ctagtgagca tacattccaa
    11461 ttgcttaccc aagtttcagg acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc
    11521 caaacattca atcctcaaca ttacgccata gaattggcta aaaaacaaaa ttatgagcag
    11581 ttttatcagc aggaaatgca tgtacggcat cgtggaggct atcctcctta ttactttact
    11641 gtgaaaataa ccgccagcca tcctgaagaa caagtagcgg caaagaaaat ctttcagatt
    11701 gccaatcagc taaaagaagt gttaagccct caaagcttgc tattagggcc aacgcccagc
    11761 atgattttga gagtgaaaaa ccgatattat tatcaactaa tcatcaaata taaacatgaa
    11821 ccgaatcttc ctcatgtttt ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa
    11881 ggtctttttg tggcaattga caacgaacca ttaaacttta tttaagaaag ggccaaatta
    11941 tgcgctatcc tatattgatc caccctaatg ataaattaaa aagaactgct cagccaatcg
    12001 atgtaatcac ggacgagaca atcgctttat tagacaattt atatgagaca atgattgcga
    12061 atgacggaat agggatcgct gctcctcaag taggacaaaa caagagaatt gctgtgatcg
    12121 aagtagatga aggcgagaaa tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg
    12181 aaagccttga cgtagaaggt tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag
    12241 cagatgaagt gaccgtgcgt tattatgatc gagacggaga agagatagaa gtgacggctt
    12301 ttggctattt agcccgtgcc ttccagcatg aaatcgatca cttagacggt atcttgttca
    12361 ttgagaaaat gatacagcag atcccggaag aagaacttga agaatatatg gaggaacatc
    12421 ttgatgacta agctagtatt tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg
    12481 ctgatagaat caggttacga gatccaggca gtggtaacac agcccgaccg tccagttggg
    12541 cggaaacgag taattacgcc gacaccggta aaagaagcgg ctctgaagca cggtatccgt
    12601 gtgctgcagc cagagaaaat ctccggttcg ccagaaatgg aagaaatcat tgaattggtg
    12661 cctgatgtga tcgtcactgc ggcatttggc caattcttgc ctgaaaagtt attgcaagta
    12721 ccaaaattag gggcaatcaa tgtccatgct tctttacttc ctaaatatcg gggaggtgcg
    12781 cctgttcatt atgccatcat gaatggggag aaagaaactg gtgtaacgat catggaaatg
    12841 atcaaaaaaa tggatgcagg cggaatctat gcgcaagaaa gcataccaat cactaaacaa
    12901 gatgatgtag ggacgatgtt tgaaaagctt agccttttag gaagaaagct attgctagaa
    12961 acattgccca atattttgga tggacaaaaa ccagtacctc aagacgagtc agaagcaact
    13021 ttttcgccaa acatcacacg ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa
    13081 attgacaacc aagtacgcgg aatgcgtcct tggccaattg cattcacgac ctatgagggc
    13141 atacgctgga aattattatc ggttgaagta ttggcagaaa ctacagacaa agcacctggt
    13201 acgatcatcc ggaaagacaa aaaaaatcta tggattgctt gcggtaaaca gaccgtctta
    13261 gccatcaatg aattacagcc agctggtaaa ggaaaacagg ccgtacatga tttccttaac
    13321 ggaagcggtc agcaagttca gattggtcag caggtgaaat aatgaaaaaa atccctaaaa
    13381 aaatgaaaaa atcagtacga tatatggcat tactgacatt agagcgagtg aacaagggtg
    13441 gcgcctactc gaatcttttg ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag
    13501 atgtgggttt atttacagaa ttgatctatg gtacgattag ccatcagcga ctattggaat
    13561 tttacgttgc accattgatc gctaaagcaa aaaaagtgga tgactgggtc aagacgcttc
    13621 tttatctttc tgtataccaa ttagaatttt tagataaagt ccctgcacat gctattttaa
    13681 acgaggcggt cgaaatcgca aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg
    13741 gtgtcttgag gaattatcag cgtcaaggag caccagatct gcaaggcatt gctgatccta
    13801 tcgaaagact ttctgtcaaa atcagcctgc ctctatggat gactaaacga ctggtggatc
    13861 aaatcggata cgaagaaact gaaaagctcg gtttatcgct atatcagcct agccatgcta
    13921 gtgcccgagt agatacacgc cgactttcca gagatcaggc catcactgtt ttgcaagaag
    13981 aagagatcac agctgaaaaa agccaagtca gtccatacgg tatagtagcg aaaaaagggc
    14041 atttagcttc tagctcattg ttccacgatg gcgtcatgac gatccaagat gaaagttcga
    14101 tgcttgtagc gccgtctatg caaatcgagc ctgaacatca tgtcttagat gcctgtgcag
    14161 cacccggagg taagacgacg catattgctt cttttttaga tccgtcatgc ggtggagaag
    14221 tggtcgcttt agatgtacac gaacacaaag tcaaactcat tgaagataat gcagcacgtc
    14281 taggtgtctc ttctgctgtc tacgctcaaa agatggatgc tagagaagtc agtaaacagt
    14341 tcgaagcaga gcagtttgat cggattttag tagacgcgcc ttgttcaggt cttgggctgc
    14401 ttcggcgaaa accagatatt cgttataaaa aacagccgga tgaattagcg aatttaccaa
    14461 aaatccaatt ggaaatttta gaaagctgcg caccaacttt aaagtcttcc ggtattctga
    14521 cttatagcac ttgtacgatc ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg
    14581 aaagacaccc tgattttgag aggattgatg ttttgaccaa tcctatgcta caatcatcaa
    14641 tcgaagagaa aatgttgact ttgtatccgc atcagtttta cacagatgga tttttcattt
    14701 gttgtttgcg caaaaagtaa tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag
    14761 gaagacgacg taacacaaat caagactatg ccagtgtatt taccagccgt tcaggtctca
    14821 agcttgctgt attagcagac gggatgggtg gtcatcgtgc cggagacatt gccagtcaga
    14881 tggcggtaac gaatctaggc gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa
    14941 tcgcccaatg gttcattcaa tcgattcaag aagaaaatat gctgatttat cagcgtggac
    15001 aagaacagcc ggaatataac ggtatgggaa caacaatcgt agcggccgct ctgtcagatg
    15061 actggttcac gattgccaat gttggggata gcagagctta tttgattcgt gaagataaaa
    15121 tcatgcaatt gacggaggat cattctttag tcaatgaact agtcaaatca ggagaaatca
    15181 gcgaagaaat ggcagtgaac catccaagga aaaatatttt aacacgttct gtcggcatgc
    15241 ctggaacagt cgaagtagat gtttctactc atctttggca actaaaagat cgactgctgt
    15301 tatgttcaga tggcttgacg aacatgctgt ctgaagaaga aataggaacg atcatcaatc
    15361 aagaaggccc attgtctcat aaagtaactg agctgattga tcgtgccaat gaagcaggcg
    15421 gagcggataa tatcaccgtt cttcttatcg aatataagga ggacagtgta tgatcgagtt
    15481 gggcaaaaaa ttaaatggcc gttatcaaat cactggaaat atcggtagcg gtggtatggc
    15541 aaatgtattt ttggcgcacg atttaatctt ggatcgtgat gtagcagtta aagtcttacg
    15601 atttgacttt caaaatgacc agactgcgat tcgacgattt cagagagaag ctttagcagc
    15661 aacagaactc gttcatccaa acattgttag tgtttacgat gttggtgaag aagacaacat
    15721 gcagtatctg gtgatggaat acgtcaaagg aatggacttg aaacgatata tccaaacaca
    15781 ttatcctgtt ccttatgaaa cagcagtaaa tattatgcag cagatccttt ctgctatttc
    15841 tttagctcat aaccatcaaa tcatccaccg agatttgaaa ccccaaaacg tactgatcga
    15901 taatgaaggt gttgtaaaaa tcacggattt cgggattgct atcgctttat ctgaaacatc
    15961 gatcacgcag acgaatacga tgctaggatc tgttcattat ctgtcccctg agcaggccag
    16021 aggaagtatg gcaacgaagc agtcagatat ttatgcttta gggatcatcc tttatgagat
    16081 gttgacgggt agcgtaccgt ttgatggtga atctgctgta acaattgctt taaaacattt
    16141 tcaagatgat cttccatcta taaaagcact tgatccaaat gtgccacaag cactggaaaa
    16201 cgtgatttta cgggcaacgg ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat
    16261 gtctgatgat ctttctacag tattatcacc agctcgtgca aacgaagaaa aatggcagcc
    16321 tcatgtgatg gataatgaaa caaaagttat cacacccctt acggaagatg caccaatgcc
    16381 tgattcattc aaatctatgc ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga
    16441 ggaggaagaa gttctgcctg atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct
    16501 attagctatt cttgctgttt taggaatcgg aacagctgta ttctttgctt ctggcgggcg
    16561 aggagaagtt gttgttcctg atgtttctga tctttctgaa gcttctgcta gagaaacact
    16621 cgcaaaagcg aaattagaag tagcaacaaa aacagaagaa attgcagatg acaaaatcga
    16681 agaaggtaat gtagtgaaga cagacccggc agcgggaacg acagtcaagc aaaaacgcga
    16741 agtcactctg tatattagta gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa
    16801 aagttatgaa gatgccgaag caagtttgaa agaattaggg ttttctgctg acttgattca
    16861 aaaagaagag gaattcagtt cagaggtaga aagcggaatg atcatttcgc aatctccatc
    16921 agaagataca gaagtcgatc ctaaaacaga cacgattact ttcacagtca gcaaaggacc
    16981 agaaacagtg actttgagta gtcatgtagg agaaaactac gaaactgttc gtaattggct
    17041 tgttacacaa ggattcaata tcaataatat tagtgctact tatgactata gcgacactgt
    17101 accatcaggt tcgatcatca gtcaggatcc agcctcgggt gaagttgtag cagaagaaac
    17161 ttacatcaat tttgtcgtaa gccaaggaac agagcctaaa ttgaatgata tctccggcta
    17221 tacaaaaagc gaagcacaaa gttatttagc tagtgtcggt gcagaatata tcgggcatga
    17281 gacttccgaa tattcaaaca cggtggataa agataaagtg attcgtacag taccaggggc
    17341 aggaacgaca attaccaaag gaatggttgt gaacgtaatt tactctaaag gaccagatcc
    17401 agcgtcttct agttctgaaa aagactcttc agacagttct agcagctcaa gtagctccag
    17461 tagcacaagc aactcttcaa aagaagaatc ttccagctcc acaagttcat cagctagcac
    17521 ctcagaaagt tcaaaataag tgctgaataa aatatatcag aagggaatgc gacattgttt
    17581 tgttgcattc tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt
    17641 gagaagtttg catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg
    17701 gaggtggcac ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac
    17761 gcaaacggag agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg
    17821 ccgcttgtcg gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg
    17881 gaaatcttgc cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga
    17941 atcattgtca caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta
    18001 gttaccttgg aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca
    18061 gacgacccag aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg
    18121 attgtaccca atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta
    18181 tcggtcttta tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg
    18241 gaactgaatt tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca
    18301 cgtcatgtgg aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc
    18361 tcgattgact ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta
    18421 gaagcttctg tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa
    18481 gtaaaacata gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa
    18541 ttcttacaag aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa
    18601 taggaggaaa caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta
    18661 caaagagaca ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg
    18721 gatggccaat ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct
    18781 gttactaaat taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca
    18841 gcatttgcaa aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt
    18901 catcgggcac ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca
    18961 ggaacaccag tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg
    19021 actgtcaatc ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg
    19081 gaactcgctg atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga
    19141 atcgttccag aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcaggg
    19201 tcatatatct atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta
    19261 gactaaatga aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg
    19321 ctgtccaaat acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca
    19381 ggctacccag tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag
    19441 gaagtatttg caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat
    19501 acacagttag gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt
    19561 ggagctacgg gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct
    19621 cgcttcaaac cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat
    19681 tatttgcctg gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc
    19741 tgcttgactc ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa
    19801 gaagtagaat atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt
    19861 tctttttcag atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa
    19921 aaaaatttgt ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa
    19981 gaggagcaac ttaccttttg tttcacggca tttagtgata aaattgaaca caagagtcta
    20041 attaattcga ggtgtctttg tgaagaagtt caatcctaat aaaaatatta ttattacctt
    20101 ggttattgtt atcatcgtgg tgacgatact aagtgtgacc gctgcgcagc gagcgaatga
    20161 aggaaagccc aatttcttcc aatccatcgt taatgacagt gttgcaatgg ttgataaaac
    20221 aatctctgcc ccagtacggt gggtacgaaa tggagtagat tctgtccata atcttttttc
    20281 aacttatgcc gaaaatgagc ggctaaaaga aaaaatcgat agctatgatg acgtttcaca
    20341 gcaaaacaag aattacgaga gagaaatcga agcgttgaaa aaagaacttg agttgaatca
    20401 gacgctgact agttacgaaa aagtaacggc aaatgtcatt actcgttcgc ctgacagttg
    20461 gcaggacatg ctgatcgtag acaagggttc taaagacggt atcgaagcaa atatggcagt
    20521 catgtcgcaa aaaggattga ttggccgagt gatcgaggtc aatacggctt cgtctaaaat
    20581 cgaattactg tcatcctcta atgaaagctc caatcatttt ccagtacggg tatcttcggc
    20641 taatggcgaa gcgtttggtt tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt
    20701 gacccaatta actggtgata cggatatcaa agaaggggat gttgtccaga catccggtct
    20761 tggagggaat tctccagcta acttgccgat cggtacggtt attaaaacga aaccagatag
    20821 ttatgggctg gatcgggaag tttatgtgaa accttatgca gaaatgtatg acgtgtcagt
    20881 tgtgacgatt gtccaacggc tagcggagga ttagtccatg ttgaagaaag aaacaatgaa
    20941 gtactatctg ccaatcgttt tgttcttttt gatgttgata gatggtcatt taacaagaat
    21001 gctaggggag tggtcgaaag gcacctatat gtcaaatgcc cactttctga tattggcatt
    21061 attatgttgc agtatggcgt ttgaaaaacg ttatttactg attaccacga ttgttctcgg
    21121 ggctatctat gacgcttact atattggcgt tatcggtatc tatgcagtag ctctcccttt
    21181 aattgtatgg ttgatgtatg taatgaaaga cgttatccat gtcaacatct ttactgaatt
    21241 tttcagtatg atcatctttg tcacgggtta tgaattgttt acgatggtgg tccagttgat
    21301 ttttaaatta gcagtagtaa ataacacgta ttttattaca aggtttttag gacctacact
    21361 gctgttgaac atgattatat ttgtattatt catttttccc tttaagaaat tattcagtga
    21421 tgaataattt ctttttttat ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag
    21481 aaatgctaga cttaacgcgg ctttttcatg atcattttct gaaacgaaca gttttgtcat
    21541 ttttaaaagt tgttttgaaa catttttgta acataactat tatatcgttg acacactgct
    21601 atggtacaat ttttatatcg ttaagaaaga cttaaatata tttagatatt tattaaatta
    21661 agccagtcgg aggaatgaag agtgaaaaag agtttaatat cagcagtaat ggtatgttca
    21721 atgaccttga ctgccgtagc atcaccgatc gctgccgcgg cggacgattt tgattctcag
    21781 atacaacagc aagatcaaaa gattgcagac ttgaaaaatc aacaagcaga tgcacaatca
    21841 caaatcgatg ccttagaaag ccaagtttct gaaatcaaca cacaagcaca agacctatta
    21901 gcaaaacaag atacattgcg tcaagaatct gcacaattag taaaagacat tgcagattta
    21961 caagaacgta ttgaaaaacg tgaagataca atccaaaaac aagcacgcga agcacaagta
    22021 agcaacacaa gctcaaacta catcgatgct gtattgaatg ctgattcatt agcagatgct
    22081 attggacgcg ttcaagcaat gacaacgatg gtaaaagcaa acaacgactt gatggaacaa
    22141 caaaaacaag ataaaaaagc tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa
    22201 gaattagctg aaaaccaagc tgctttggaa tctcaaaaag gcgatctttt atctaaacaa
    22261 gctgatttga acgtactgaa aacttcatta gctgctgaac aagcaactgc tgaagacaag
    22321 aaagctgact tgaaccgtca aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa
    22381 caacaacgtc tagcagaaca agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa
    22441 gcacgcgaac aagcagaggc tgaagcacaa gctactcaag cttcatctac tgcacaaagc
    22501 tcagcaacag aagaatcatc agctacacaa tcttcaatga ctgaagaatc atcatcagct
    22561 acacaatctt cagcaacaga agaatcaaca acacctgaat cttcaacaga agaatcaaca
    22621 gcgcctgaat cttcagcaac agaagaatca acaacagtgc ctgaatcttc agcaacagaa
    22681 gaatcaaaaa cagtgcctga atcttcaaca acagaagaat caacaacacc tgcgcctaca
    22741 acaccatcaa cagatcaaag tgttgataca ggaaacggca caggaagtag tactccggct
    22801 ccaacgccaa caccaacacc tgaacaacca aaacctgtaa cacctgctcc agctccttct
    22861 ggaagtgtga atggtgcagc aatcgtagca gaagcttaca aatatattgg tactccatat
    22921 gtttggggcg gtaaagatcc aagtggcttt gactgctcag ggttcacacg ctatgtttac
    22981 atgcaagtaa ctggtcgtga catcggtggt tggacagttc ctcaagaatc agctggaaca
    23041 aaaatttcag tttctcaagc aaaagctgga gacttgttat tctgggggtc acaaggcgga
    23101 acttaccacg tagcgattgc tttaggcgga ggacaatata tccatgctcc tcaaccaggc
    23161 gaaagcgtaa aagttggttc agttcaatgg tttgcccctg actttgctgt cagcatgtaa
    23221 gaatgattaa tcaaaaagag ttagcccatt ctggactaac tctttttt
//
LOCUS       DACEMK010000045        22964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEMK010000045 DACEMK010000000
VERSION     DACEMK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.045"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..783
                     /locus_tag="IW715_001487"
     CDS             <1..783
                     /locus_tag="IW715_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1022096.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1161..3203
                     /locus_tag="IW715_001488"
     CDS             1161..3203
                     /locus_tag="IW715_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1022097.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3214..3834
                     /locus_tag="IW715_001489"
     CDS             3214..3834
                     /locus_tag="IW715_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1022098.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3846..4304
                     /locus_tag="IW715_001490"
     CDS             3846..4304
                     /locus_tag="IW715_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1022099.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4323..4601
                     /locus_tag="IW715_001491"
     CDS             4323..4601
                     /locus_tag="IW715_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1022100.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4626..5993
                     /locus_tag="IW715_001492"
     CDS             4626..5993
                     /locus_tag="IW715_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1022101.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            6008..7153
                     /gene="dgoD"
                     /locus_tag="IW715_001493"
     CDS             6008..7153
                     /gene="dgoD"
                     /locus_tag="IW715_001493"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ1022102.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7180..7671
                     /locus_tag="IW715_001494"
     CDS             7180..7671
                     /locus_tag="IW715_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022103.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            8114..9313
                     /locus_tag="IW715_001495"
     CDS             8114..9313
                     /locus_tag="IW715_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1022104.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9808..10566)
                     /locus_tag="IW715_001496"
     CDS             complement(9808..10566)
                     /locus_tag="IW715_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1022105.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10734..11669
                     /gene="pfkB"
                     /locus_tag="IW715_001497"
     CDS             10734..11669
                     /gene="pfkB"
                     /locus_tag="IW715_001497"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1022106.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11666..13111
                     /locus_tag="IW715_001498"
     CDS             11666..13111
                     /locus_tag="IW715_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1022107.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            13139..13594
                     /locus_tag="IW715_001499"
     CDS             13139..13594
                     /locus_tag="IW715_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1022108.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            13613..14587
                     /gene="lacD"
                     /locus_tag="IW715_001500"
     CDS             13613..14587
                     /gene="lacD"
                     /locus_tag="IW715_001500"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1022109.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14698..15259)
                     /locus_tag="IW715_001501"
                     /pseudo
     CDS             complement(14698..15259)
                     /locus_tag="IW715_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            15403..16017
                     /locus_tag="IW715_001502"
     CDS             15403..16017
                     /locus_tag="IW715_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1022110.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            16258..16641
                     /locus_tag="IW715_001503"
     CDS             16258..16641
                     /locus_tag="IW715_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1022111.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            17004..17513
                     /locus_tag="IW715_001504"
     CDS             17004..17513
                     /locus_tag="IW715_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1022112.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            17613..18263
                     /locus_tag="IW715_001505"
     CDS             17613..18263
                     /locus_tag="IW715_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022113.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18714..19652
                     /locus_tag="IW715_001506"
     CDS             18714..19652
                     /locus_tag="IW715_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1022114.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19665..20717
                     /locus_tag="IW715_001507"
     CDS             19665..20717
                     /locus_tag="IW715_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1022115.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            20983..21618
                     /locus_tag="IW715_001508"
     CDS             20983..21618
                     /locus_tag="IW715_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1022116.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            21809..22600
                     /gene="msrA"
                     /locus_tag="IW715_001509"
     CDS             21809..22600
                     /gene="msrA"
                     /locus_tag="IW715_001509"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1022117.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7303 a         3682 c         4842 g         7137 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc
      841 attgttttta gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg
      901 aatagatact ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa
      961 tattctaccg aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca
     1021 tttgatataa cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag
     1081 tgtgacaaaa aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt
     1141 ttgtcaggag gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta
     1201 aaaaccctga aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa
     1261 tcaattacgc gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc
     1321 agagaagtca ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc
     1381 ctgattacga cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag
     1441 cattgatagt gatgactctg atcgctaata tcgattacat cagtctggat catttggtag
     1501 atgcggttga tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca
     1561 tcagaaatta tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt
     1621 cggaacaccg tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc
     1681 gagaaatgat tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga
     1741 tccataacat ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc
     1801 agacatcagc gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt
     1861 ttcccgatga tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa
     1921 caggttggag tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca
     1981 atatttttga gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga
     2041 tcagccaaat ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag
     2101 actttgaacg taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact
     2161 tgagtttagg agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga
     2221 ttgatttgat gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa
     2281 atgatgaatt agatctttta tcctattact ttggctatca attaagtaat cataataaat
     2341 tgaataagca acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt
     2401 tagtacgagc caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg
     2461 tgcgtgattt ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa
     2521 aaagttctat gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt
     2581 tacgatatcg agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat
     2641 tgttaacaat cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac
     2701 tgcagtactt tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc
     2761 cttcattgac ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt
     2821 gggaacgagc agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc
     2881 gttttatcgc tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa
     2941 tgaagacttg cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca
     3001 ttttagtttg taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc
     3061 cgctttcatt ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg
     3121 ccaaagatga ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc
     3181 aactattaag acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg
     3241 acagtgatca tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag
     3301 gaatttgacc atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat
     3361 atacatgaac taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg
     3421 acattagaac aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat
     3481 atgttcacaa aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc
     3541 gcaatgactc cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta
     3601 tttcctgcag cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag
     3661 ttaccactaa tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa
     3721 ggtgctagct atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta
     3781 aatgttaaaa acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga
     3841 ggactttgga actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag
     3901 atagtgaagt cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg
     3961 aatatattca agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac
     4021 caccaggaat cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa
     4081 ttgcgactct taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac
     4141 caatccaaat tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac
     4201 aaaaaattgt tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca
     4261 atgatactga attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca
     4321 taatgaaacg aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa
     4381 caaaagttag agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat
     4441 tgatggaagt tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg
     4501 atggtcaaac aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg
     4561 ccgatcagac aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg
     4621 aaaaaatgaa aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta
     4681 tgttgccagt catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt
     4741 ttaaatcagg tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta
     4801 tgacgaccaa tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag
     4861 atgctttgga tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc
     4921 caatcttgat ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac
     4981 atacgctgga tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt
     5041 attttgtgac taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga
     5101 cttttaagat ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg
     5161 tttctttacc aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc
     5221 tgctggatcg tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat
     5281 atttgggatt ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg
     5341 ttttagccgg ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt
     5401 tggttttgat tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg
     5461 ctgcacaaaa atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg
     5521 ctgcagttgt tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa
     5581 cgattgcatt agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg
     5641 ttgttccttt ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata
     5701 tcattattgg cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa
     5761 tgctgacagc aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct
     5821 catcatttgc tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca
     5881 aaccaatcat cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg
     5941 atggtatcaa aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg
     6001 gtttaaattg aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt
     6061 taagatctcg actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac
     6121 agaaacggtt gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt
     6181 tgaaatcgag cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa
     6241 tggaaccatc gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa
     6301 tgttccggtt tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg
     6361 gattggtgga gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg
     6421 ctttacggcc gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa
     6481 agttgatgaa gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga
     6541 tattggtgtt gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc
     6601 tttggagcca ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca
     6661 ctttaaagaa gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac
     6721 tcgttggcaa ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt
     6781 ggctctttgt gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga
     6841 catggctgtt gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt
     6901 agactcatgt acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa
     6961 aggttttgat ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta
     7021 cgtagatcta ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga
     7081 aattgcacaa gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat
     7141 cgctgagtgg tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc
     7201 acagtttttt taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg
     7261 tttgctcaga gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg
     7321 ttgattttct ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc
     7381 attttgatca atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga
     7441 gtattgaacg gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat
     7501 aaatcattta tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa
     7561 gatcaaactg aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta
     7621 gatcaaagtt cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac
     7681 cgaaattatg atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg
     7741 aagtatacaa tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa
     7801 tcgattactc gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta
     7861 agagatgatg gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc
     7921 aagctagtat gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag
     7981 atgttttaat tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact
     8041 tttaaaaata gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata
     8101 aggtgggaga tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag
     8161 tttgcatttt ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt
     8221 gatttatttc aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt
     8281 ttcaacggtg cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact
     8341 gatcaccgtg ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt
     8401 gattccgaat ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca
     8461 atcatttctc aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg
     8521 cttttcaaga gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct
     8581 gttagcgatt tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt
     8641 tttcgttatt ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa
     8701 agaacaggat cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt
     8761 aggtttcagt tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt
     8821 tgaaaatatc cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg
     8881 cgaattaccg ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg
     8941 gctgtctgtt gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc
     9001 gatcctgatg atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc
     9061 agcttcggct tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac
     9121 gatgatcatc atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct
     9181 gctgcagcat ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg
     9241 tgctttatta actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc
     9301 tcttgttgag tagcataaaa atacttagat actagtttta tataaataac atatagtgat
     9361 tattggcgta attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa
     9421 acctcatcta aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac
     9481 tgattgtaat ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt
     9541 attataattc tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac
     9601 ttcgaactgc ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc
     9661 aatgcactat tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga
     9721 gaatacaaat agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat
     9781 tttaggtaca gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc
     9841 ataggagtca agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata
     9901 atctgggaag aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat
     9961 caaacttgaa tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt
    10021 ttcagctgcg gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc
    10081 atctttggat aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca
    10141 atatttaccg gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc
    10201 tcgggcaaaa atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt
    10261 cccgctttct accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt
    10321 ctttattcct tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc
    10381 ttttaacgaa tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac
    10441 gacggttgct gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat
    10501 ttcgagtatg tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt
    10561 atccatattt tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta
    10621 cttcaagttt atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa
    10681 catttacatt cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag
    10741 tttatacatg tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac
    10801 catcccaagt caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg
    10861 tttcattgat catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt
    10921 ttaccgggaa ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta
    10981 gcgtaccggg aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat
    11041 tcaaattggt taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc
    11101 aaatacaaaa gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg
    11161 tctcacccga aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga
    11221 ttattgattc aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa
    11281 aaccaaacga ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat
    11341 atattcatta tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag
    11401 gaggagaggg aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag
    11461 gtaaagtagt caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat
    11521 atttaaaagg tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa
    11581 ccgcttttcg taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg
    11641 aaatcaaaga gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg
    11701 tccaacagga attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg
    11761 caaaggggta tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac
    11821 accagaagaa ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc
    11881 agaacgtttc gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc
    11941 tgaccaattg atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac
    12001 agttgacgct gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat
    12061 ttataaaaat ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt
    12121 aattattgca atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac
    12181 ttacaatccg actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt
    12241 tcctatttta gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg
    12301 gtttgttggc ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc
    12361 aggtttctta gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa
    12421 acaattggat ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg
    12481 tgtagtcatg tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt
    12541 cttggcagga ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc
    12601 agcatttgat atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt
    12661 ggcacaaggt aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt
    12721 gattactgga tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc
    12781 tggtttagtc aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc
    12841 ggctaaagat ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt
    12901 actgacatat atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc
    12961 tgttgttact ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg
    13021 agtaatttta gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc
    13081 tgttgaaatg aaagaagcaa ccaacaacta aaggttattc tagaaagaag gatttcaaat
    13141 gactttgatc aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt
    13201 aatgaacttc cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt
    13261 ttacgaaaaa ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc
    13321 catccctcat gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa
    13381 cggtaaggtc gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt
    13441 tattccagat tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact
    13501 tttgatgcac aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc
    13561 tgaactttta aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt
    13621 aacagaaaaa aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc
    13681 gttagcaatc gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc
    13741 agcaaccgat gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata
    13801 tgcttcagca atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa
    13861 aaatgctggt ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg
    13921 tttaccggat attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc
    13981 ttgtaagttc ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa
    14041 ggcgtttatg gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga
    14101 attggtttct tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa
    14161 accgcataaa gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt
    14221 tttgaaagtg gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt
    14281 tgcttatacc aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct
    14341 gccatttatt ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt
    14401 tgcaaaagag gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa
    14461 tggtgtagaa ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca
    14521 aggacgcaag aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa
    14581 attgtaagaa agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa
    14641 tttgagcttt taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta
    14701 aggcatgata ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt
    14761 agagtatgca aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat
    14821 ggtagatggt gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc
    14881 agatttttaa ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc
    14941 cgtggttgat gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt
    15001 gagcgtatag atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt
    15061 ggaaaaagct attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa
    15121 atgtcgaacg gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt
    15181 ttttatctac taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc
    15241 ggcgatcctc ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg
    15301 ttatccgaca atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata
    15361 taagtatagc atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag
    15421 caactggaaa agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt
    15481 gattggtata ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta
    15541 gaattgaatc gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt
    15601 ttattagcag acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat
    15661 gttaaactat ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac
    15721 gacaccaatg acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt
    15781 gtctttttag ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa
    15841 gaatttgaac gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg
    15901 tatcaatttt atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag
    15961 atccttaata aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt
    16021 tttttactat cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc
    16081 catttagatg aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta
    16141 aaaatagatt tctgtatcca acaataaaaa tatttttgta tttattagta tatatacttt
    16201 acaaaaattt gtatatatac taattttttt gtaggatcaa tgaaatgagg ggaaaatatg
    16261 ccaaaatata aagaaatagc cttggctcta aaagagagga tcgttgaggg aaaatatcag
    16321 gaaggttcta aattacctga gcaagaaagt ctggctagag agtttaaaac aagccgtgtt
    16381 actatccgca aagctataca actccttatt gatgaaggtt tgttatatac gagacgtgga
    16441 tctggtactt ttatacgaag caatatcaga caggacaatg agaatgtcac tcaagtaaat
    16501 aatgtatttg gcacttcctc acaagaaaat acaaatgtga caagtaaaat aatccgtttt
    16561 gatgtccggt ttccgacaga ttatgaaagg cggctttctg caacctactt ccgatcccct
    16621 tcctgcaaat catgggtata aaaaataggc atattaagcg ggctaggagg agaaatattc
    16681 acatatcatt ttttcattcc gcaagctgcg tcaagttatt tggaatttgt agaacagatg
    16741 agagaggtag aagcagcact acaaactact tttcaataag taggaattat agaaatttta
    16801 ctcaaaaaat cttcattaaa aaatctgact aaataagttg agaaaacaga atatacttca
    16861 atatccagtg atttataaat atcattcact catgataccc agctccgtat cataagtgat
    16921 gcggttttta tcattgatcc taaaaatctt agcaagacaa aaagtacgta agaaagtaaa
    16981 gactaaatac ttcggaggaa caaatgaata acaatgatag gttattgcga ttgcgttatg
    17041 cgattgattt gaaagatacg gatttgatta aagcttttga gctaggcggt gtaccactga
    17101 ctaaagaaga agctcaggcg gtactgacca aagtacagga taagaacaaa gataatgcag
    17161 aaaataatgt gtatgaaaaa accatcaaca accaagtttt tgacgcattc ctaaatggcc
    17221 tgattctctt ggcgcgtggt ccgcaaaaag ataagccgat agtagacacg ccgcaaaaaa
    17281 gcaaggaaat caaatatatc aacaatgtgt tgctaaaaaa attaaaaatc gccttgtcta
    17341 tgaccactga tgatatttta gaagtttttg ctgaggcaga actctatcca tcaaaaggcg
    17401 aaatcggggc atttttgaga aaagaaggcc aacgaaattt taagccttgc ggagataaat
    17461 atatgcgtaa ttttctcaag gggttaggaa tttataatcg gagaaaagta taaagaaatt
    17521 tcatgagaaa tcaaaacaat ggttgttttg atgagagcta gtttttgtat gagattaaaa
    17581 tattatctta atcgaggagc tggattggtt ttatgaatga tgaatataag aatgatgaag
    17641 ataagatgct ttttgaagag atagagaatc gatgtaggct caattttgag cttcggggaa
    17701 agatgtcttt aatacaacaa aaaaagtatt tagcgaataa atcagagttt actctgggac
    17761 atgttgaaaa attaatctcg gattggatta gtagtagaag cgaatttaca aaaataaaac
    17821 agccgattaa atttgacatg aaaaaacttt tgttaaataa gagtgagata ggtaatagag
    17881 atcaatatat tagagctaaa gggcaagaaa taattgattc attaggtgaa atgaggtcat
    17941 acaattattt gtatgtaaca catagagctg atggaatggt cattactgtg gggaaatctt
    18001 cttctaatga tattttttta gatggagatc ttttttatca actcaataca aatcacttgt
    18061 ctggcactga aaatataatt ttaaggacgg aatatggcaa tgaaatattt gcaaaatatg
    18121 atgagatttt aaaaaattat ttggactggg cttggataat tccggttgag tcaggagatg
    18181 ctaagaaact ggagagatta ttgggcgatg aactaattaa taaaaaagta ccaattttaa
    18241 attattattc ccatagacaa taaaaggcta tttttcatga taatactagt tataactgta
    18301 aaaatggaag aaaatctggg aaagaagttt ttaaataagt gtattgttcg attttaataa
    18361 ataataagca ataccatccg tctgtgttgc gcaacatcgt actacaatga atattttttc
    18421 acaaaaagaa aatcatccat cgaatgaatg gaaggtttta gtatacacga ggttaatgcc
    18481 ccctctttta taatcagagg agtgaggttt tttctatgaa tgtttctatt ttcttattgc
    18541 tagtctagtt tctcttacga tgaaattggg attctattcc agtttttatt gatttgctga
    18601 taaaataact tgaatggtgg atataaacat gataaataag agaagaataa caaaacaagg
    18661 ttgtgaaatg attctcaaat gaatataata gaaagaaaaa ggaggaagaa tcaatgaaaa
    18721 tagcaattgc aggcgctggc gcaatgggaa gtcgctttgg gttaatgctt caccaaagtg
    18781 ggaacgaagt attattaatc gatggctggg cagaacatgt ccaacaaatc aaagagcatg
    18841 gattacaagc aaattttaat ggtaaagagg tagaagcaaa actaccaatc gttcttcaat
    18901 ccgaagtaga aaaagaagat caagttgatc tgattattct atttaccaaa gcgatgcagc
    18961 tggaaaaaat gctgcaggat atccaatcat taatcaaaaa agatacagag gtcttatgtc
    19021 tattaaatgg tatcgggcat gaagatatta ttgagaaatt tgtaccaatg gaaaatatct
    19081 atattggaaa taccatgtgg acggcaggtc ttgaaggtcc tggtcaggtc aaattatttg
    19141 gaagtggttc agtagaatta caaaatttag gtgatggaaa agaagcagct gcaaaaaaat
    19201 tagcagacaa attgtctgaa tcaggattaa atgctcattt ttctgacaat attcactatt
    19261 ctatttatcg caaagcatgt gtgaacggaa caatgaacgg actatgcaca atcttagacg
    19321 ttaatatggc tgagctagga aaaacatcga ctgctcataa aatggtggcg acgattgtca
    19381 atgagtttgc caaagtagca gcagtagaga agattgaact agatgtccca gaagtcattg
    19441 cacattgtga atcttgtttt gacccggaaa caattgggtt acattatcct tcaatgtatc
    19501 aagacttgat taagaaccat cgattaacag aaatcgatta tatcaatggc gcaatttcta
    19561 gaaaagggaa gaaatatggt gttgcgacgc cttattgtga tttcttgact gagcttgtcc
    19621 atgcaaaaga agatagtttg aacgtaaaat aaaggagaga ttgaatggaa acttcacaaa
    19681 aaatatcacc gaaaattttt ctgaataaag tcctcgctgg tacagcaaca ggaatcatta
    19741 ttggattgat tcctaatgca gtattaggtg ccattttaaa atattttagt gcgtatccct
    19801 ttgcggtaac gatcgcacag ttagcagtta tttttcaact agcaacacct ttgatcattg
    19861 gtggtttgat tgccttacaa tttggatttg atccgatgag aatgatggtt gtagccggag
    19921 cttcttttgt tggttctggt gtagtcaaat tcacacctga tttgggtgaa acgggtgttt
    19981 acgttggtgc tggtacaggc gatattatta atacgatgct gacagcatcg attgctgtcg
    20041 ggctgatttt actgattgga aatagttttg ggtcagtgtc gattgtgtta acaccgattg
    20101 ttgtaggtgt aggtgcaggt ttacttggtt tttatttatt cccttttgta acagcaatta
    20161 cttctgcaat tggatcattg attaataact tcactacctt acaaccgctg ttaatgtcta
    20221 tcttgattgg ttgttccttt gcctttttga ttatctctcc tatttcaaca gtagcaatcg
    20281 gaatggcaat ccaactagat ggtgtttctg ctggtgcggc tgcgatgggc gtagctgcaa
    20341 ccacttttgt tttagtagtg aactcttgga aggttaataa gtcaggtgtg acaattgcga
    20401 ttgctttagg cgctatgaag atgatgatgc ctaatctatt tagaaaacct gtgattctta
    20461 ttccttgttt agttagtgca gtgattacag ctattcctgt cgctctattt tctatttcag
    20521 gtacgcctgc ttctgctggt ttcggcgtgg tcggacttgt tggtccgttg gcttctttag
    20581 atgcagggct aaatggcttt cttgttgttc tttgttggtt agtcatccct gtttttgcgg
    20641 cactaattac tcaattttta tgcgagaaag tatttaaact gtatgatcga gaagaagtct
    20701 tcaaattttt aggctagtag attttactaa gaaattttca atcaatggat gcgtaagtga
    20761 gatgtcaaca acatctgact tacgtttttt tgcctttttg agtaggagat tgatgctgac
    20821 aactgaactc ttcagtgcca ttcgattttt taaaatgtag aagaaactca cctttatgtt
    20881 attgattgtt caaaaggcaa ctagaataaa aatatgtgtt aaaatgagaa aaaatttgat
    20941 tgttggatgt atcgaataat cactaaaaag taggagaaga aaatgatttt tttgcaaaaa
    21001 tatggttttt attttttatt attaggtgtg attagtgatt tatcgacacc gtatatcctt
    21061 ggcctttttt atcccaaact gaatcaaatg actaccgtaa ttagtgtgtt tggtgacgta
    21121 gatagtccag tcagacgtgc tttcttggtc tggtcagttg tatctggact tttttttgtt
    21181 ctttcgttgc ctgcgttgta tcacctattt attggaacat caaaaacact tgcaatactt
    21241 gctgttgcaa cagttggttt atatgggata ggtgattgta tttttactgg gttattcagt
    21301 atcaatacga atgaaagctc ttggaatcta tcgacttgga tccataatat aggttctggg
    21361 ctaggctatg caggctttct acttttccca ttgttacttg ttctgcttta tcgtcaaagt
    21421 ggtcaaggta cgcttagcca tttttatctt gttttaacag ttattagttt actaattgca
    21481 ggtctttacg gattagcacg gattccgtcg attagtcagt ttgccttctt taagcaattg
    21541 ggcttctttc aacgcttaag ttttttcttt aactatttgc cgatgatttg tttaagtttt
    21601 tttcaactac gaaagtagaa aaagcagtgt gaagctgatt aagtaaaaga atttctctat
    21661 gggtaaccat tcgtgtggat gtgttcgact aagaatagag atgaggagag cgaaaagcaa
    21721 gttagtctta acttgttatg cgttgtgcta aatttcaggt agtcattttt tgaatgaata
    21781 agaagaaaac ttgaaagagg gtgacttcat gttagatgtg gagcagatgg aaatagacct
    21841 ttataatatt attttaaatc gaaatacaag agaatgggaa agaacacaat ttgaagaggc
    21901 aaaagaagac ctagcaaaaa tgtcgataaa gcaggtgata gaaaaactag aggtcaactt
    21961 gcgtccgcta gcactacgag ataatttgac accagatgcg atggattttt atttaagaat
    22021 cacaggggag tcagcgagtg caaaggctta tgattatgcc aaacataaga taacttcacc
    22081 cttataccaa gaacgggcga tttttgccgg tggctgtttc tggtgtatgg ttgagccttt
    22141 tgaaacaaaa ccaggtgtaa tctcagtgtt atctggttat acaggtggag agaccaaaaa
    22201 accaaattac gatcaagtca gtggtggata tactgggcat gttgaagctg ttgagatcat
    22261 atttgataca cgtcaaataa cctatgaaga gctgttggag atttattggc aaatttcaga
    22321 ccctacagat gcctttggtc aatttcaaga tcgggggaaa caatatcgtt caattctatt
    22381 tgttgttaat gaaaagcaaa ggcaattggc tgaaaaatca agagaacaac tgattcaatc
    22441 taaccgattt agccgtccta tcgttacaga gattcgccaa gcaaagactt tttggccagc
    22501 tgaaaatcat catcaacaat tttatcagaa gcaacctaaa agatataaaa agatcaagca
    22561 ttcgcatcag cttcatttat tttttcagcg acatagatag aggaaggttg tggcgcaaag
    22621 cgttcagctc cgacaaaata atgagcaact gacaaaaata gcttttcata tttttggaca
    22681 gttatgaatt atttcgagga gttgctttga gaacaccgag gaaaaggttg tggcgcaaag
    22741 cattcagctt caagtcgcaa ggaaacagga ggtatgacaa gtcttttgtc acaactcctg
    22801 tttttttact agcctttatt tccatgtgaa agagaattcg aattgtagaa tcttgatttt
    22861 tctatggaat tttgtagaga atcatcttga taattaatag acacctaatc gaattccaca
    22921 ttaatgatga actaaattcc gtataaaagc gcaaacaata acta
//
LOCUS       DACEMK010000046        21561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEMK010000046 DACEMK010000000
VERSION     DACEMK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.046"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(310..1806)
                     /gene="lysS"
                     /locus_tag="IW715_001510"
     CDS             complement(310..1806)
                     /gene="lysS"
                     /locus_tag="IW715_001510"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1022118.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1917..2921)
                     /gene="dusB"
                     /locus_tag="IW715_001511"
     CDS             complement(1917..2921)
                     /gene="dusB"
                     /locus_tag="IW715_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1022119.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2922..3809)
                     /gene="hslO"
                     /locus_tag="IW715_001512"
     CDS             complement(2922..3809)
                     /gene="hslO"
                     /locus_tag="IW715_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1022120.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4026..6137)
                     /gene="hflB"
                     /locus_tag="IW715_001513"
     CDS             complement(4026..6137)
                     /gene="hflB"
                     /locus_tag="IW715_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1022121.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6227..6772)
                     /gene="hpt"
                     /locus_tag="IW715_001514"
     CDS             complement(6227..6772)
                     /gene="hpt"
                     /locus_tag="IW715_001514"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1022122.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6772..8217)
                     /gene="tilS"
                     /locus_tag="IW715_001515"
     CDS             complement(6772..8217)
                     /gene="tilS"
                     /locus_tag="IW715_001515"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1022123.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8337..8807)
                     /locus_tag="IW715_001516"
     CDS             complement(8337..8807)
                     /locus_tag="IW715_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1022124.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8853..9278)
                     /locus_tag="IW715_001517"
     CDS             complement(8853..9278)
                     /locus_tag="IW715_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1022125.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9345..9614)
                     /locus_tag="IW715_001518"
     CDS             complement(9345..9614)
                     /locus_tag="IW715_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1022126.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9625..11220)
                     /locus_tag="IW715_001519"
     CDS             complement(9625..11220)
                     /locus_tag="IW715_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1022127.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11235..14756)
                     /gene="mfd"
                     /locus_tag="IW715_001520"
     CDS             complement(11235..14756)
                     /gene="mfd"
                     /locus_tag="IW715_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1022128.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14773..15333)
                     /locus_tag="IW715_001521"
     CDS             complement(14773..15333)
                     /locus_tag="IW715_001521"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1022129.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15686..16660
                     /locus_tag="IW715_001522"
     CDS             15686..16660
                     /locus_tag="IW715_001522"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1022130.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16830..18230
                     /locus_tag="IW715_001523"
     CDS             16830..18230
                     /locus_tag="IW715_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1022131.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18308..19030)
                     /locus_tag="IW715_001524"
     CDS             complement(18308..19030)
                     /locus_tag="IW715_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1022132.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19109..19615)
                     /locus_tag="IW715_001525"
     CDS             complement(19109..19615)
                     /locus_tag="IW715_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1022133.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19738..21537
                     /locus_tag="IW715_001526"
     CDS             19738..21537
                     /locus_tag="IW715_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1022134.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5990 a         4710 c         3869 g         6992 t
ORIGIN      
        1 cagttatctt atcatgtcgt tagtaacatg tcaataactt ttttgttatg tttttgaata
       61 aatattttct tctgtctttt ggacaagtaa taatatacca actaaattta gcttggtcaa
      121 cgaatttttt gtattttttc aagttttttt caataaacga ttattcataa cttttctttc
      181 tttatcgttc gttttcgtct tctttaatgt ctagaatagt cggcattagc ttttgaactt
      241 tcgttattat ttattccttt ctacacagca aaagctgcct aaggaattcc ttagacagct
      301 tttattcgat tatctcattg ttgggaatag caaaacatcc cgaattgatt gtgcatcagt
      361 tagcaacatt actaatctgt cgatcccgat gcctagacca ccagttggcg gcataccgta
      421 ttctaaggct tccaagaagt cttcgtctac accgtgagct tcgtcatttc ctagttcacg
      481 ttctttttct tgcgcttcga atctttctct ttgatcaatt ggatcgttca gttctgtaaa
      541 ggcattagca aattcgcgcc ccactatgaa tagttcgaat cgatctgtga atcgaggatc
      601 ttctggattt ttcttcgcta aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg
      661 ttgtgtcaat gtttcttcaa caaatgtttc aaagaattca ttgataacat gtccgacacc
      721 catagcttcg gttacttcta cattgtgttt ttcggctaaa gctaatgctt cttccaatgt
      781 catttcttgc cagaagtcta caccagtttg ttctttgatt gcatctacca tatggatgcg
      841 tttaaagtca ctttctaagt cgacagcttg tccatcatat gtgatttttg ctgttcctag
      901 tactttttca gctgcgttac ggataatacc ttctgtcaga ttcattacat cattgaaatc
      961 agtatatgct gtgtatgctt ctaacatcgt aaattctgga ttatgtgtag tatcaattcc
     1021 ttcattacgg aaaacgcggc cgatttcata aactttttcc attccgccaa caattagtcg
     1081 ttttaaatga agttctagcg caatacgcaa atataaatca atgtccagtg cattatgatg
     1141 cgtgataaat gggcgtgcgg cagctccacc tgcctcattg tgcaatactg gtgtttctac
     1201 ttcaatatag ccattgccat ctaaatagcg acggatttcg ctgataattt ggcttcgttt
     1261 cataaatcgg tcaaagcttt cgcgattact gattaaatcc aagtagcgtt gtctgtagcg
     1321 ttgttcaata ttcgttaagc catggtattt atctggcagt ggacgaagtg cttttgacaa
     1381 aatcgtgatt tctgaagctt tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc
     1441 agttacacca aagaaatctc caaggtctgc atgtttgaat acttcatagg cctcatcacc
     1501 tacttggtct tttcttacat aaatttgaat ttggccttca cgatcttgta gatgcgcaaa
     1561 tccagctttc ccttttcctc ggcgagtcat catacgacca gctacactag cggtcaatcc
     1621 catttctgct agttcttctt ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt
     1681 gtgcgtgcgt tcaaatcgtt ttccaaatgg atcgattcca tcttcacgca attgttccat
     1741 tttttgacga cgtacaagca tttgatcatt taaatcttct acttgatgtt gttgttcctg
     1801 tgtcacaaaa gttcctccat tctcatgtta atctctttcc tataatgcca atgaaacaca
     1861 aaaaaagcaa gccgaacttg gaatttcggc ttgaaaattc acgaaaaact tagaaatcac
     1921 aacgtttgtt tcaatagtac tttttccgct ttttctacaa attgatccag caggtcattg
     1981 atttcttgct gtttttctgt ctgattgatt gctactttca ctttcgctgc tcgaggaatc
     2041 ccttttaaat aataagccgc atgtgttctg aattctctac aagctacttt ttctcctttg
     2101 agatctgcta agcgttgtaa atgaagttta gctgtagcaa ttttttcggc tggtgttggt
     2161 tcaggaatca gttctcctgt ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg
     2221 cccagcgctg cacgaccaat catcacaccg tctgcaccga catagtcaag catccatttt
     2281 gcatcttctg gcgttttgac atccccgttc cccataaatg ggatcgtcaa atgttttttc
     2341 acttctttca atacttccca gtccgctttt ccttcataca tctgaacccg tgtacgtcca
     2401 tgcatagcga tggcacttgc tccagctgat tgtgctgcta atgcattttc tacagcaaag
     2461 acgtgctctt catcccaacc aatacgcatc ttgattgtta ctgggatatt tactgctgaa
     2521 gaaacagctt ggaccatttc gtatactttc tcaggatcta aaagccattt tgctcctgct
     2581 tctgctttga ttactttatt tactggacag cccatattga tatctattat atcggcagct
     2641 gtgttttctt cgacaaattg agcagcttca actagactat ctttatttcc gccaaatatc
     2701 tgtaaactta atggatgttc tgtttcttca atgtggagca tctctaaagt ttttttgttt
     2761 ctgaaatgaa taccttggtc actgatcatt tcacatacga ctaagcccgc accaaattct
     2821 ttgacagtga ctcgaaatgc tgaatttgtt attccagcca tcggtgcaac aacgacacga
     2881 ttagggattt ccacattgcc aattttccat gtgtgttcca cttaaatccc tcctaaggct
     2941 tctttcttca atccatctaa ttcttcttcg ctatacttgt atttgttatt acagaaagaa
     3001 caaaccgctt ctgcaccgtg atcttcgtct atcattgctt ggatttcttc tgcacctaac
     3061 gcgataattg cttctgcaaa tttttctttt gaacagtcac atttgaattg aacaggcatt
     3121 ttttccaaaa tgttcaattc ttctgtacca agcaatcctt tcaatatttc ttctggtgtc
     3181 tgtccttcat ctaagagggt agagattctt ggtgtcgttt ttaattgttc ttcgatcttg
     3241 ctgattgttt cttcatcggc acctggcatg atttggatca tgaatcctcc cgccgtcttg
     3301 atgctgtcgt cagtattgac caatacactc agtccaacag ctgaagggat ttgttctgag
     3361 atagcaagat agtaagtaaa atcttcagcg atttctccag aaacaatcgg cgtttgccca
     3421 gaaaaaggtt cttttaatcc taagtctttt gtgacagtaa acattccttg tgttccaact
     3481 gctccccgca catcgatttt accttcacta tttagaggaa gactgagatg cggatttttg
     3541 atgtaacctt tcacatctcc ctttccattt gcatctacta caattcctgt tgctggacca
     3601 tcgccttgga ttcgaacagt tagtttgtct tctcctttga gcgtcgcacc aagtagcaga
     3661 ctaccgacca atgtccgtcc caaagcagct gaggaggtgt gccaagtatc atgacgtctt
     3721 tgtgcctccg ctacggtatt tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga
     3781 gcaagtgcct taaccaaata atctttcatt tgttatgtac tcctttttcc gtttttaaac
     3841 tcatagtgaa tcatactagc aactactgct tttttcaact ttggaatgct tcttttaaaa
     3901 ataattcaga atcatctaga aaacaggaca tcaccctatt tacaacttaa ataaagaaat
     3961 ctcccgaaaa ccggtttttg ttccggtttt cgggagtcat atcagatcaa ttgacctata
     4021 tttttttatt tgtattgatc gtctgaatga ttatcttctg attgttgaga atctgaatca
     4081 tgttctgctt tattttcctc attcagttca tgttttgctt cttcaaaatc acgtttttct
     4141 tccgcttgtt tttcagcgtc tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc
     4201 gcttcttttt cgcttggata atcatttgac tcagtacctt caggcatcac accgtgttca
     4261 aacaatgact tgattgcttt ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc
     4321 agtttatgct gtgcacgatg agcttcgatg atttctcgag ctttatcatg agcttccatc
     4381 aagatttttc gtacttcttg atcaatctcg aaagcgactt gttccgagta agcttttgtt
     4441 tgaccatagt cgcggccaac aaacacttga tggtttcctt catattgaac aggacctaaa
     4501 cgatcactca ttccatattc tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag
     4561 tcattggaag caccagttga ttggacattg aagatgattt cttcagctgt acgtccacca
     4621 agtaagccta cgatttgttc aaacatatct tctttcgtca tcaagaactg atcttctttt
     4681 ggtaaagcga tcatatatcc gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg
     4741 acccgagcgc gactcaatac taatccgaca atcgtatgac cagcttcgtg ataagcaacc
     4801 atttcgcgtt cttttttgct gattacgcga tcttttttag ctggacctgc aatcacgcga
     4861 tcttccgctt catcgatatc agaagcatcg attttctttt tatttcttct agcagctact
     4921 aacgctgctt cattcaagac attttctaaa tctgctccgg caaaacctgg tgtctgttga
     4981 gccacaactt tcaagtcaac gtcatctgcc atcggtttat tccgtgcatg gactcgcaag
     5041 attgcttcac gaccttttac atctggacgt ccaactaaaa tctggcggtc aaaacggcct
     5101 ggacgtagta acgctgggtc tagtacatca gaacggttgg ttgcagcaat gacgattact
     5161 ccttcatttc cgtcgaagcc atccatttct acaagcaatt ggttcaatgt ttgttcgcgt
     5221 tcatcatgtc cgccgcccat accggcacca cgttgacgac ctaccgcatc aatttcatca
     5281 atgaaaatga tagctggtgc gtttttcttc gctgtttcaa acaaatcacg aacacgactt
     5341 gctccaacac cgacaaacat ttctacgaaa tcagaacccg agatcgagta aaacggtact
     5401 ccagcttctc cggcaacagc ttttgctaat aaggttttac ctgttcctgg aggtccttct
     5461 aaaagtacac ctgccgggat acgggcacct aattcaacaa atcggcgagg atctttcaga
     5521 aattcaacaa cttcaactag ttcttgtttt tcttcttccg cacccgctac atcagaaaaa
     5581 cgtacgcgat tagctttttt gtctgcttct ttggcttttg atttaccaaa gttcatgaca
     5641 cggccgccac cgccaccgcc gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg
     5701 atgattacga ttggcaagaa gctaagcaat agggaaaccc atataccgct gcttgattct
     5761 tcttttactg ttgtagcaac tttgttttct tttgccagtt ctgttacttg gctcaatgtt
     5821 gtatcatttg gcaaaatgat tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag
     5881 atagctaacc caccgctgtt agcgacggtc tgctgttctt tgtattcacc agtgatctta
     5941 tatacaccat ttgcaggctg cacttcaaat tctttgatct tgcctgcttg tagttgttct
     6001 gtgaattttg agtactcgat atctgacgat cgctggccgt tattaccaaa aatgaaataa
     6061 acgaccatta ccatcgctaa gattacgagg acataataca aggcattttt catcatgccg
     6121 ttgttctttt tgttcatagt tgccctccct aagcgtcaat ttaatttaac agttttttat
     6181 caatcctgac cattctattg taccataatt taattgagat acagcattaa tttgattgat
     6241 aaacactagg tttcaataca ccgatgtacg gaaggttacg atatgcttct gcataatcca
     6301 agccataacc gaccacaaat tcatttggta cgttgaagcc aacataatct gcttcgatat
     6361 ttactactct tccttcaggt ttgtcaagta gtgtaacaat tttgactgat tttgctttgc
     6421 ggtatttgaa aagatccact aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa
     6481 cgatcaacag atcgcgacct tctacattcg tatctaagtc tttgacgatt tttacttccc
     6541 ctgacgagac tgttgcattc ccgtagcttg aaacatccat gaaatccaat tccaaatacg
     6601 tatcgataga acgaacgata tctgccatga atggtaccgc tccttttaag acacctacta
     6661 ctaggggatt ggtgttttgg taaatttctg ttaactcttt gcctagttct ttgcaacgaa
     6721 cttgtatctc ttcttgtgag atcaaaattc tctcaatatc cttttccaac atcaggttct
     6781 ccttccaatt gcttcttttt gatatttgta aagaagtatg tagtgtattt tatcagtttc
     6841 ttcacgaata ctcaaatatg aaaaggcaaa aggtatcaag ctatatatat ttcttttggc
     6901 atctgtaatg atccaagcat gctgcctttg tgaattaggt actttctgat cgataaaata
     6961 tctagatact ttttttgtga gattctctgt taatttaatg cggtctcctg cttttctttt
     7021 ttcgacatac agttgcacat cagcaggaat ccaaatgtca tgagaaaata gagaccatga
     7081 atctgttgct ttgatgtccc ctgcattttc aggtttgacc agaccaatcc attctgtttc
     7141 attcaaatat attccttgtt ctggaatcag caacaagcgc tggtcttctg tctggctagt
     7201 tgcttgtttt tcttttttag agagtacaac aaaatgatac tctcttttca gtatccaatt
     7261 attgccaata ctgagttgcc attggctttt tttctgttgc cactggtcta aaatcgactc
     7321 catttgttgt tctttcaaaa taattcctgt ttttgagaat gctttgtcga aaaaggagta
     7381 aaaaaaatag tatcgttcag catctgtcat ttcttcaatc atttctgcag aaaatcgaaa
     7441 ttgttcctcc cactgttcga cttccttttc gatcaactgt cccatatatt tttgaatgac
     7501 ttggtctgcc cattggatct gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt
     7561 ttcttgtttc aattgaggaa ctaccaaatg acgaagccga tttctctgga tatctaacaa
     7621 ttggtttgtc tgatcctcaa agtaaacaag ctgcgattcc gctgcataag tatataggtc
     7681 ttctttgcta aaactcagca acggacgaat cagtctgccc gtagcaaatg gttgtacttc
     7741 tttgattcca gaaaaagtat ttaactgtcc cccacgaacg attttcatta acaccgtctc
     7801 gatttgatca tctccgtgat gagcagtcat caacgtgtca tattgtcctt cttccatcac
     7861 gcgagtgaat tcttgataac gaaagttacg agcttctgtt tctatggcgg atttggctgg
     7921 atcttcccaa atacttacat ataaagggag attcttctct tcacaatatg agcgaagata
     7981 ggcagcttct tctgctgatt cttctcttaa ttggtgattg atgtggacga tacctaaaga
     8041 aaaaccgatt tggcgttgca cttgtaaaag taaatggagc aaaaccatcg aatcaacacc
     8101 acctgatacc gctgctaaaa ttctcgatcc ttgttgccag aatccttgtt cattcccgtg
     8161 ccgaataaat gactgctgta atagcggtgc ttttttcccg cctttttcaa cgttcatcct
     8221 ttccacaccc tttcttccta aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac
     8281 ctgccctctg ttgatactat gacccaaata gtcgcactat cattatttgc ggctttttag
     8341 ctgcgacgtc cgccacggcc accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag
     8401 cggtcatcgc tatctttcaa aaatgaactc atcaatgaat cgaagtcttc tttttgagct
     8461 gaaggttgac tgcgagaaaa agattttttc tgtgctggac gatttcggtt gtcacgggga
     8521 tctttttgtt gatatttttt aggacggctt tcttctccag attcagcagg ttgttcctgc
     8581 gctttccgga tagatagtcc gatttttcca tcatcattta ctgaaataac tttaactgtt
     8641 acttcatctc ctacacttaa aacatcatgg atgtctttta catagccatt tgacacttcg
     8701 ctaatatgaa ctagacccgt ttttccagca cctagatcaa taaacgcacc aaaatttgtg
     8761 attcctgata cttttccttg cagttttgct cctacctcga ttgacataaa aaaaatgttc
     8821 ctcctaatta tttccctttt ttatattcct gcttattttg cagttgtaga actagttgat
     8881 tgatctgttt catttgtcga gctgtctgta tttgattcta atataggata aactttttca
     8941 ccgtcttttg agtaaaagaa tttgcttctg gcaagtttag ctacatactc atcatctttc
     9001 agcaatgaaa catcttggtg cagctgatca acgtctgcgt tgacttcttt catttcagta
     9061 tttgcttcga ctctcaattc gttcaattga tgcatccttt gcatgtcatg gaaaatctgc
     9121 acacctatta tcgcgaaaat gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt
     9181 cgaaaaatca gttgacgttg ttgttgctga aattcagcat actttttctt tgcatattcc
     9241 gtatctagcg cagcgatttt attgcttttt ttcgtcactt ggattcgctc caatcttttt
     9301 gattgtcttt ttcattatac caatatcgtg aaagcttgtc taatctattt ctctagtgtt
     9361 tctgtgcgat tttcagaaat aatttcgtac attttttgtg catcttcttt tttggttgat
     9421 tcatgtaatt ccgtaatttt cacttccaac gttttgttgc caaactggac tttcaaaata
     9481 tcgcctactt tgacatcact ggaagatttc gccaatttac cgttgatttg aattcttcct
     9541 ttatcagcta cttcttttgc tactgatcgt cttttgatga ttcgggaaat ttttaaaaat
     9601 ttatctaatc gcatatctta ttcctcactt tttaattttt ttatttaatc gcaggatttt
     9661 cttaccaaaa ggcaacatta gccattctcg tactgtaaac agacgaagac gaatcactgc
     9721 ataaatgaac acagaagccc cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga
     9781 acgatgagtt acaggaccaa atactatatt caatacacca taatacagca gcagtactac
     9841 tgtcatacaa agaaggctga acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc
     9901 atttagctct tttgtttcaa ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag
     9961 ggtagacagg cttgcacctg ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc
    10021 ttttaccaat agtccccatc ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg
    10081 agcaatactt tgataagctt ggatgactgc agtgaaagca atggaaaaaa caaataatac
    10141 aagcgctgca tttcccgcat aatctttaaa caacgcataa ttgatataag ggagcagcaa
    10201 ggctaatccc aaagacgcag ctaatgctaa agcggtcgtc aatctaagat agattttggc
    10261 tgtctgtaaa aactgacgat agatccggtt ggtcaaatga cgagtcaacg caggcaggaa
    10321 tgtcgcactt aatgcagtgg caatcactaa acctaattgt actaaaggct gacctcgatc
    10381 atagacccct tttgctatct tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt
    10441 ttttacaaag aaagagtcga tcagctggaa aaagataagc aatccactat aaatcgaaac
    10501 cagtccgcct tctaccagaa aacgccggat caagggcttt gacggctgtt tgaacaatgg
    10561 aaaccgtcgg aagcttaacg ctcctccatg aatcttttgt tcatagtagt gaaggatccc
    10621 atatgcgcat aaaccgccaa aaacagctcc actcattgct gccgtccctg tttggtacac
    10681 cgtccatcca agctttctaa aagcaaacgc actacagaca atcacaaaaa cacgaacgaa
    10741 ctgctcaact acttgagaaa cagcagttgg ttccatcaag aatcttcctt ggaaattccc
    10801 tcgataaaaa gatagaccag gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg
    10861 tttcaattgt atgtctccca tcatccaagc aatccagctg ctaaatgcaa agacaaacaa
    10921 ccacaaaaag acacctgtcc aaaatactaa gggataaaga ttttgcaaag cttgctttcg
    10981 ctcgaaagga ttctttcttt ctgcaacgta ctttgatatg aactgcggaa gaccggataa
    11041 agctaaagtc attgctatcc cataaattgg ataaacttgc tgataaacat aaaaaccttc
    11101 gtcgccgact aagttttgga ggggaattcg gtaaagggca cttaatactt ttgctatgaa
    11161 agaagcaatc gtcaatacaa atgctccttg catgacgcgc cgcatttctt tatgatccaa
    11221 atcattgctc cttcctacgc tgacagatat ttttgctcac gcaacgcttt gacaaacttt
    11281 tggatttcct gaagccatac tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc
    11341 atctgttctt tttcaacgcc tagactggct tttagttttg tcgctgtcag tgcttcgaat
    11401 aactgttcca cactatagcg ttttgttcca actttactta gagtaaagac tatatgcgcc
    11461 tcctgtttcc ttatcgtttc taggagcgct cgatcgccat ccattttgat ctgcccaatt
    11521 gtcaagagat gtgctacttc ttctggatat tctccaaaac ggtcgagcag atcatcttct
    11581 agttcatcta gcatttcttg cgaatcaagt tcacgaattc gtttatagat ctcgattttc
    11641 tgccgttgat ctgccacgta tgtttcaggt agatacgcat cgacaccgag gtcgatctca
    11701 acagtggtct tctccacttg actattcttt ccttgtttac gagaaacagc ttcgcttagc
    11761 atttgagaat acatatcgaa accgactgca tcaataaatc catgctgctg agcacctaag
    11821 agattacctg caccgcgaat ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt
    11881 tctgtaaaat ctttgattgc ctgaagacgt ttttcactga cttcgttaag gatcttctgc
    11941 tgctcataca taaagtaagc atatgcgaca cggctgcttc ttcctacccg ccctcgcaat
    12001 tggtataacg tggataatcc catataatca gcgttctcaa cgaacaacgt atttgcgttc
    12061 gggatatcta ctcctgtttc aatgatggtt gtagtgacaa gaacatcata ttgaccttca
    12121 ataaattcaa aaagcgtatt ttctaattgc gcctctgtca tttgcccatg agcataaccg
    12181 atccgtgctt ccggaacgag ttcttggatt tcttctactt tttgttcgat cgtttctacc
    12241 cgattatata aatagaatac ttgtccacct cgtcccattt ctcgatggat tgcttcgcga
    12301 atagccccag gatttttttc cataacatac gtttggatcg gataacgatt cgcaggcggt
    12361 gtttcgatta ctgaaagatc ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc
    12421 ggtgttgcag tcagagtcaa aacatccact tgtgcacgta actgtttcaa tcgttcctta
    12481 tgttttacac caaaacgttg ttcttcgtca atgatcagta gacccaaatc gctaaattca
    12541 atatcttttg acaaaattcg gtgggttcct acaacaatat caacttgacc tgtacgcagt
    12601 tgttcgatgg tttctttttg ttgctttctt gttctgaaac gacttaatag cccgatattt
    12661 acggggaaac cttcaaatct ttccagcatc gtttcatagt gctgctgtgc caagatcgtt
    12721 gttggaacta aaaaggctac ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc
    12781 gctacttctg ttttcccata accaacatca cctacgagta gtcgatccat tggtttttct
    12841 ttttccatat cccgtttgat ctccgctgca cttctcaact ggtcatcagt ttcagagtag
    12901 gggaaggcat tttcgaattc tttttggtag gcgtcatcag gtccaaaagc atatcctttt
    12961 tctgactctc ttgcggcata taatttgatt agatcgtcag cgatatcctc gatttttgaa
    13021 gagacttttc gcttggtttt cgtccattca cttccgccta gcttattgat gcgaggcgct
    13081 ttggcttcag aggcgacata tttttggatc agattcagtt gagtaacagg aataaacagt
    13141 ttgtcgtcgt tttgatacaa aatagtcata tagtcttggt gaacgccatc tacttctagc
    13201 gtttccatcc caatgtattt cccgatacca tgattggcat ggacaacgta atctcctgct
    13261 tttaactcat tatagctttt caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt
    13321 tttttcgtcg ttttttggaa aatttctttt tctgtgatag ctactatttt ttcttgagga
    13381 agttcaaaac cagcctgcaa agctccttca actaattgta cctgtcctct gatcaatgca
    13441 tttttttctg tttctactac taatatgtct tcatctcgaa gcatttgttc tgctttgcgt
    13501 attctgtcct ttgtcggaat aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc
    13561 atttctgttt tcagcaatgg catttgtccg aagaactgtt gcatcgtccg atattgaaaa
    13621 ttatggattg cttggaaacg aagatttccc atcccttttt ggaagagtga gaaaaaggtt
    13681 gtggtgaact tttccttctg cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa
    13741 aacacccgca attcgctgat tttttgagtc tgccattctg ccgcttccct ttcgatttcc
    13801 cgatttgttt ccatgattcg ctgataatca tcaacgacta gtaaactatt tgaagggaaa
    13861 taatccaaga tcgtcacaga ttcttggtaa agaagatctg agtagaattt tgcttcttct
    13921 gtcgggacac ctgcttgcca gcttgaagtc agctgaccga agtaatcttg caaaaactcg
    13981 cggtctgctt cttctgtggt cgcagcaagc cgtttttcta gcattttttg catttgctct
    14041 attccatgtt ctagatcctc tggtccgtag acttgttcac tcgttgggga aacccagatt
    14101 tcttccgttt tttcgattga gcgttgtgtt tctgcttcaa aataccgcat agaatctatc
    14161 tctacatcaa atagttctac acgaacagga taggttgtcg ttaacgggta aatatcgata
    14221 atgcttcctc gaatactgaa ttctcccggt tttgcgacca tctcttctcg tacatagccc
    14281 atcaaagtca attggcgagg aagattttcc acgtctatct catcaccaat atgccaattc
    14341 aattgcgcct gtagccaagt tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc
    14401 aacaaataga tacctgcttt tttttcttga agtgcgttaa gcgttgcaac tctctcgcct
    14461 cgcgcttctg gagaagaaaa ggccatttca gcagaaagca cttcatccac tggaaataaa
    14521 tggatctgat cttgcggaat cacatgttgc agatcttctg ctaactgatt cgcataatac
    14581 aaatttggca tcagaataat catgttttta tttgttttct taaacgctcg agcaaataga
    14641 agtgttttcg ctgagcccgc taatcctgtg attagttgtc taggcatttt ttcttctaaa
    14701 ttgctgaccc attcttttac ttgcggatct tttccgacta attgaataat atccattcct
    14761 tttcctcctt cctcaattga atcgattcat cgcatccacg aacgaatgcc cttctatgag
    14821 atattcgact gctttaacag attgacacat gctttcttct atcaaaggct gattttcttt
    14881 gctgaaagga ctaagcacat gctggactac tgtttttttc ccttctggcc tgccgattcc
    14941 caccttgatt cgatcaaaga cattcgtatt caaatgggaa ataatacttt tgataccgtt
    15001 atgcccgccg gcacttccct tttgacggag ccggattttt cccactgcca gatccaaatc
    15061 atcgtagatt acgactaatt cctctggata aatcccaaaa taagtcatta gtgggccgac
    15121 tgcacgacca gattcattca tgaatgtttg tggtttgact aaaaggatct tttctccatt
    15181 atgaaaaaat tcggcaactt ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc
    15241 gcgtgcaatt ttatccacga ccataaagcc gatattgtgt tttgtatatt ggtatttggt
    15301 acctgggttt cctaacccga cgatcatttt catttttgtt ttccacctaa ttctaaaaag
    15361 cagcacaaaa ggctggactg ctaattagcg gtccagtcca tcgagctttt ttctttttta
    15421 tacttgtcaa tgcatagtat aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa
    15481 taaatgataa ataaattaaa aatttcttca aagtttaatg aaataattga tatataaaaa
    15541 tagtgaaata acgcacatgt aagcttttac atataataca accttgttat ctgttatatt
    15601 ttatagccaa atagcgtgac atggggcttg agaaatgtta gcataagcat gggaaaaaac
    15661 aaatcaatgg aaggattgat actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa
    15721 agttattctt gttggagacg gcgccgtagg ttctagctat gcctttgctc tagttactca
    15781 aaatatcgct caagaagtag gtattgtgga tatcaatact gctaaaactg aaggagacgc
    15841 aattgacttg tctcacgctt tggcatttac ttcacctaaa aaaatctatt ctgctactta
    15901 cgcagacgca catgatgctg acttagttgt tattacagct ggcgcaccac aaaaaccagg
    15961 tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca
    16021 aatcgttgaa tcaggattta acggtatctt tttagttgcc gctaacccag tagatatttt
    16081 gacttactca acttggaaat tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac
    16141 ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg
    16201 taacgtccat gcttacatct taggtgaaca cggagattca gaattcccag tttggtcaca
    16261 tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa aacaacccag aaatcgatga
    16321 agaagctatg gttaacctat tctttggcgt acgtgacgct gcttacacaa ttatcgagaa
    16381 aaaaggcgct actttctatg gtatcgcagt agcattagca cgtatcactc gtgccatctt
    16441 agatgatgaa aatgctgttc ttcctttatc agtattcatg aatggcgaat atggattgaa
    16501 cgacatctac attggtgcac ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga
    16561 aattccttta agtgacagcg agcaagaccg tatggctgct tctgctaagc aattaaaaga
    16621 aattctagat gaagcttttg ctaaactaga cgctgaataa tctttgaaca tgacggaaaa
    16681 catctattcg gtgttttccg tttttttatt ttatcaccct tatggtttct aaattttttc
    16741 ataaagtaga gataaccttc gtcttttcct ctaaattatg ctatcatgag accgttccac
    16801 cctaaacttt tacttaagga gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga
    16861 aacatgttgc tttgctttca gttcttggtg ttctcttagt gctgatcagc ttttatacct
    16921 atcgcagtac atactacaca aagcattttt tgccgaatac agaaataaat ggaataaatg
    16981 tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa agaagcttat tctgataaaa
    17041 aactggctat taaagaagat ggaaaagtct ggcaggaagt accaaaatcc gagctcggct
    17101 acaaagatga tttcacttcg gagctttctc cgatcctaaa tgaacaaaac ggatggactt
    17161 ggggaatgac ttatgtctca gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc
    17221 accaaaaact agacactacc gttcagacat tgacaactaa gctgactgat ttgaacaaag
    17281 accggacacc tactactgat gcaacgatcg aaaaagcagg tgactctttc gttatcaagc
    17341 ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg
    17401 taaactctgg taaagataca attgaattga ctgaattcaa agaaaaacca aaagtaacat
    17461 ctgaagacag ctcactgaaa gaacaactcg catccatgaa tgctatcgca aatgtaaaag
    17521 ctacttacag tataaacggt gagactttcc agatcccatc aagtgatatt atgagttggc
    17581 tgacctacaa tgatggaaaa gtcgatttag atacagaaca agtacgtcaa tatgtaacag
    17641 atttaggcac aaagtataac acaagtacga atgacacaaa atttaaaagt acaaaaagag
    17701 gagaagttac ggtcccagtt ggcacttaca gttggacgat ccaaacagac agtgagacag
    17761 aagcattgaa aaaagcgatt ctggcaggcc aagattttac tcgttcaccc attgtgcaag
    17821 gtggcacaac cgctgatcat ccattgatcg aagatacgta tatcgaagtt gatctggaga
    17881 accagcatat gtggtattac aaagacggaa aagtggccct tgaaacagat atcgtttccg
    17941 gaaagccaac tacacctaca cctgctggag tcttctatgt ttggaataaa gaagaagatg
    18001 caactttaaa aggaacaaat gacgatggaa caccttacga gagtccggta aactactgga
    18061 tgccgattga ctggacaggt gttggtatcc acgattcaga ctggcaacct gaatacggtg
    18121 gagacttatg gaaaacccgc ggttcacacg gctgcatcaa caccccacca agtgtaatga
    18181 aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat
    18241 atataaaaag aaagcacccg tacgattggt acaggtgctt tctttttatt atctaacaat
    18301 gtgctactta catttcagca gcagtaaaga cttctgaaac atcatcatcg tcttctagct
    18361 tatctactag acgttccaat tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg
    18421 gaatcatcgt caattctgct tgcgccaaag tgaactcttt ttccaactca tctcgtacag
    18481 cagcaaaatc ttctggtgca gtataaatct cgaatacttc aggagaagtt tgcagatctt
    18541 ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc
    18601 gttcaatagc gatatatcct ttgcggtcaa acaagtaact gactgaaccc gtttctccca
    18661 gtgatccacc gtttcgagta aaagctactc ggacatttgt cgctgtccga ttacggttat
    18721 ctgtcaatgc atggactaag attcctactc cacccggacc atatccttca tacgtgattt
    18781 catcgtaatt cgtttcatct acggcactcg tagccttttt aatcgcccgt tccacattgt
    18841 cgttaggcat atttgcagct tttgctttgt ccacaacaag ccgcaaagct ggattcatag
    18901 atggatcagg accgcctgct ttagctgcca tataaatttc acgagaaagt ttctgaaaga
    18961 ctttccctcg ttttgcatct tgggcatttt ttcttccttg gatattatgc catttactat
    19021 gtcctgacat gatttttcct cccttgttct tttgagaata cacttctatt ctagtactat
    19081 ttcacaaaaa aacaaagcca cagccaatct ataaacgctt cttgaatgcc gcaaaaagca
    19141 cacataatat cacagcagtc agagcaccga tcgaatccaa catgacatct tggaacatag
    19201 gtgttcgtcc accggtcatc atctgatgga attcgtccaa acctgcgtac cctgttgctg
    19261 aaagccaagc aaggatcgct gtcagccacc aaatttttag cttggggaaa acacctaaaa
    19321 acaagctgcc tcccaaaata aaataggtac cgaaatgtgc gcccttcctt aaaaagaact
    19381 ctaaaaattt ggcgtagccc agatggtcga tactcaccat acttccacca taattaaaac
    19441 gtatacccgc taatgtgtct ttaaatggct gattaggcag ccagctttct aatcgcgaaa
    19501 cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa taagattata acgactatca
    19561 gtgcgattac taaataaata tttccgtttt tcatttgttt ttttgaagaa ttcataagtc
    19621 acctctgtat tttatcttag actttttttg aagaaacaac aatcattttc atagagactt
    19681 cttccataaa aaagtgctat acttaatttt gtattacaat atgaagggag ttttttcatg
    19741 agttattcat taaattggaa tctagattct attttttccg gaggcagtca ttctgatgct
    19801 ttgaaccaac ggatgaagca attggaagac caaatgaacg agtattatca ccgcgtaaca
    19861 aaatggtcac ctagttctga caatgcagag cagttaaacg ctattcttca attgcaagaa
    19921 acgattacga atggttttac ccaatgcagc agctatatta ctgcattgct ttctgccaat
    19981 gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg
    20041 caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat
    20101 cagatgcttt ctcaatcttc gtttgaaaca attgcttttc gtttaaacga gattcgtcgt
    20161 gatggctcac aattattatc agaagctgaa gaaaacatta taaatacgct ttctttagat
    20221 ggtctaaatg cttggagcag tcattatgat acgattgtag ccagcatttc gattcccttc
    20281 gaacaagatg gacaagtagt cgaattatct gctggacaag ccttcaataa gatgatgggc
    20341 gatccagatc caaaagtcag agaaacactt ttcgcagcat gggaagaagc ctgggaagaa
    20401 aaggcacccc tcttcacgga tactttgaat catttagatg gttttagact ttctgactat
    20461 gaacttcatg gtgtcacaga ttttctacaa aaaccattag agtacaatcg gttgaagaaa
    20521 gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca aacaacccct tgttaattac
    20581 ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca
    20641 cctattattt taggcgattt gaaagaaaaa acctttagtt ttgacgaagc agcagcattt
    20701 attatcgaaa actttaacaa attcagtcca aaaatggcta aatttgccca atctgctttt
    20761 gaaaagagct ggatcgaagc agaagatcgt cctgggaaac gtcctggtgg ttattgtact
    20821 gagttaccag aaacaaaaga atcaaggatc tttatgacct acagtaattc tgttaatgag
    20881 gttgcgacac ttgctcatga actaggacat gctttccata gcagcacgat gtgggattta
    20941 ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa
    21001 ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg
    21061 ctagatacaa aactgcaaaa tgctttagct atgttcatga atatccattc ccgattcatt
    21121 tttgaaaatc gattctatga agcacggcaa gaaggattag tctcagaaga taagatcaca
    21181 gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag ctttagctac ttaccatcca
    21241 tatttctggg ctgcaaaatt gcatttcttt attgatgacg taccgtttta taacttccca
    21301 tacacatttg gctacctctt tagtttgggc atctatgctt acgccaacca aaagggagca
    21361 aattttgaag aagaatatat ccatttacta cgcgatactg cctcaatgac gacggaagat
    21421 cttgcgaaga aacatttgaa tgtcgatctt acccaaccag atttttggca agcaggtatc
    21481 gacatggtga ctgaagatat ccaaacgttc atggaattga cagaagaatt tatctgattt
    21541 ctattttatc acaataaaaa a
//
LOCUS       DACEMK010000047        20818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEMK010000047 DACEMK010000000
VERSION     DACEMK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.048"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..1241)
                     /locus_tag="IW715_001527"
     CDS             complement(39..1241)
                     /locus_tag="IW715_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1022135.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1358..1873
                     /locus_tag="IW715_001528"
     CDS             1358..1873
                     /locus_tag="IW715_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1022136.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2072..2518)
                     /locus_tag="IW715_001529"
     CDS             complement(2072..2518)
                     /locus_tag="IW715_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1022137.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IW715_001530"
     CDS             complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IW715_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1022138.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4397..5026)
                     /locus_tag="IW715_001531"
     CDS             complement(4397..5026)
                     /locus_tag="IW715_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1022139.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5010..5474)
                     /locus_tag="IW715_001532"
     CDS             complement(5010..5474)
                     /locus_tag="IW715_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1022140.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5961..7229)
                     /locus_tag="IW715_001533"
     CDS             complement(5961..7229)
                     /locus_tag="IW715_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1022141.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7460..8197)
                     /locus_tag="IW715_001534"
     CDS             complement(7460..8197)
                     /locus_tag="IW715_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1022142.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8264..8833)
                     /locus_tag="IW715_001535"
     CDS             complement(8264..8833)
                     /locus_tag="IW715_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1022143.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8961..9833
                     /locus_tag="IW715_001536"
     CDS             8961..9833
                     /locus_tag="IW715_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1022144.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10175..11047
                     /locus_tag="IW715_001537"
     CDS             10175..11047
                     /locus_tag="IW715_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1022145.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11044..13002
                     /locus_tag="IW715_001538"
     CDS             11044..13002
                     /locus_tag="IW715_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1022146.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13253..14104)
                     /locus_tag="IW715_001539"
     CDS             complement(13253..14104)
                     /locus_tag="IW715_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1022147.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14227..14694
                     /locus_tag="IW715_001540"
     CDS             14227..14694
                     /locus_tag="IW715_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1022148.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14957..15439)
                     /locus_tag="IW715_001541"
     CDS             complement(14957..15439)
                     /locus_tag="IW715_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1022149.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15539..16402)
                     /locus_tag="IW715_001542"
     CDS             complement(15539..16402)
                     /locus_tag="IW715_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1022150.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            17000..17848
                     /locus_tag="IW715_001543"
     CDS             17000..17848
                     /locus_tag="IW715_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022151.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18019..18360)
                     /locus_tag="IW715_001544"
     CDS             complement(18019..18360)
                     /locus_tag="IW715_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1022152.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18446..19153
                     /locus_tag="IW715_001545"
     CDS             18446..19153
                     /locus_tag="IW715_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1022153.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19140..19463
                     /locus_tag="IW715_001546"
     CDS             19140..19463
                     /locus_tag="IW715_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1022154.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19552..20589)
                     /locus_tag="IW715_001547"
     CDS             complement(19552..20589)
                     /locus_tag="IW715_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1022155.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6202 a         4308 c         3695 g         6613 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc
     1021 atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta
     1081 gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa
     1141 gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt
     1201 gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca
     1261 tcatacatga tttattttac atgtaaatat gcacatgtaa aacgtggaaa ctttactgtg
     1321 atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt
     1381 attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat
     1441 tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg
     1501 ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga
     1561 tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa
     1621 agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg
     1681 actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt
     1741 gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag
     1801 aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca
     1861 gttttttgag taagggataa acagaacctt tctagaaata tctattgaag agcaaattat
     1921 cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac
     1981 ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg
     2041 ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca
     2101 cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca
     2161 ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt tccatgatca
     2221 caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga
     2281 tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca
     2341 caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca
     2401 acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga
     2461 ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc
     2521 tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta
     2581 agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca
     2641 aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct
     2701 aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta
     2761 cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta
     2821 cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca
     2881 agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc
     2941 atttgataac ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct
     3001 tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca
     3061 atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca
     3121 cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat
     3181 tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt
     3241 tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt
     3301 tgtttagagg aagacaaccg atccggtctg atttgttgct ttttcttttt actggacact
     3361 ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc
     3421 tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg
     3481 agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt
     3541 ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa
     3601 taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc
     3661 tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt
     3721 tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg
     3781 gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg
     3841 aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc tattagtttg
     3901 agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag
     3961 acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt
     4021 atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta
     4081 tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct
     4141 actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc acctattcta
     4201 ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt
     4261 gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt
     4321 ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat
     4381 agtaactgaa aaccttttat tcgaacgtag tgatcttttc tggtgctaaa cgaacagact
     4441 cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt
     4501 tatccaaacc aaatgcctct gccaactgat ctgcttcgaa tccaccaatt ggatttgttt
     4561 catagccatg atcacgcgca actaacatga attgcatagc cgctaagcta gaatcgactt
     4621 tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta
     4681 attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg ttatagattt
     4741 cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat
     4801 cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt
     4861 cagcgaccac aaagcgccat ggttgcatat tgacagaaga aggtgcagtc gtcgcttctt
     4921 tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg
     4981 atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct
     5041 ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat
     5101 tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc
     5161 atttcacaat gctcgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg
     5221 ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga
     5281 cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga
     5341 ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct
     5401 tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc
     5461 tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca
     5521 ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct
     5581 caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga
     5641 aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct
     5701 gcagtagaaa attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc
     5761 gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt
     5821 gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa
     5881 ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc
     5941 agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc
     6001 atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt
     6061 tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct
     6121 tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga
     6181 tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc
     6241 caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc
     6301 aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa
     6361 gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa
     6421 ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag
     6481 ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc
     6541 ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg
     6601 gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg
     6661 ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg
     6721 ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg
     6781 gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta
     6841 gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct
     6901 tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc
     6961 tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt
     7021 tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta
     7081 ctctctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt
     7141 tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata
     7201 acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc
     7261 ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt
     7321 tgtttctttt tgataaattt gctttttcta cataaaaaga cgtaaatgaa gaatattctt
     7381 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctcttt
     7441 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttactga
     7501 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcggagg
     7561 tcaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
     7621 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
     7681 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
     7741 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
     7801 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
     7861 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
     7921 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
     7981 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
     8041 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
     8101 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
     8161 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
     8221 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
     8281 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
     8341 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
     8401 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc
     8461 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
     8521 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
     8581 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
     8641 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
     8701 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttcagct tggatctgat
     8761 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
     8821 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatcatg
     8881 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
     8941 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
     9001 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
     9061 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcgg ggctcccgag
     9121 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
     9181 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
     9241 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
     9301 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
     9361 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
     9421 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cgaaatcatc
     9481 ctttagcaaa gcaaaccgtc atccatccgc aggaactgaa aactgttcct ttgatcattt
     9541 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
     9601 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
     9661 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaaatattca
     9721 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
     9781 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
     9841 ttttttcctt cgctttcagc tttttaacaa cgaataagat aagcaaaaga tccaacgatt
     9901 aaataacata agtttcttaa tttatttctt ccttttattt ctgataattc cagtaaatta
     9961 ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta
    10021 acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg
    10081 gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa
    10141 acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca
    10201 aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg
    10261 atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat
    10321 ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt
    10381 atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga
    10441 acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg
    10501 tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag
    10561 tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga
    10621 cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca
    10681 tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt
    10741 ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt
    10801 acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt
    10861 agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt
    10921 gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca
    10981 tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa
    11041 aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta
    11101 cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag taacgacatt
    11161 gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt
    11221 gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt
    11281 ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg
    11341 aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt
    11401 attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg
    11461 atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg
    11521 ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct
    11581 tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac
    11641 cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta
    11701 gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga
    11761 atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct
    11821 atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gctagcagca
    11881 gcttttgctt cattctttac tggtgtgaca gaaccacttg aattttcatt tatgttcgtt
    11941 gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca
    12001 ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct
    12061 ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca
    12121 gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc
    12181 cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat
    12241 gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc
    12301 gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat
    12361 aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc
    12421 atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct
    12481 gcagcacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtgaaacg
    12541 atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca
    12601 gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga
    12661 gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc
    12721 ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac
    12781 ttaaaaggcg aaccattcac tttatatgta gaagaaggtc aaaaagtcgc aagaggtcaa
    12841 ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc
    12901 gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta
    12961 aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga
    13021 cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc
    13081 gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagctaa acgaaacata
    13141 tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc tttttcacaa
    13201 aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata cattattcct
    13261 gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt
    13321 ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa
    13381 acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat
    13441 cacttgcttc aataatttct aactctttac ctgtcgcacg ctctaatgcc ttggctaatt
    13501 cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta
    13561 gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc
    13621 ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct
    13681 ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt
    13741 cagtgaattg gtgatctggc gctaatatac tgatcgagtg atcggcatct gcaaaacttg
    13801 tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg
    13861 cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt
    13921 ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca
    13981 gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct
    14041 tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat
    14101 gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta catttataaa
    14161 ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga
    14221 caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa
    14281 atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat
    14341 gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa
    14401 accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc
    14461 tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat
    14521 tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa
    14581 aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt
    14641 ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct
    14701 ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg
    14761 gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc
    14821 atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc
    14881 aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt
    14941 cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt
    15001 tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt
    15061 ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag
    15121 gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg atgattcgcg
    15181 tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat
    15241 agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg
    15301 ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat
    15361 ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt
    15421 cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa
    15481 aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta ttttttaact
    15541 atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc
    15601 attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa ggaagccatt
    15661 ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa
    15721 aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt
    15781 ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag cggtctccat
    15841 caaatccata agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct
    15901 cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt
    15961 caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga
    16021 tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag
    16081 atttttgtat gccgtatcta ctgatttctg aaggcagata tcctgaccaa tcacgaaaag
    16141 ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg
    16201 atccatctag aagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta
    16261 gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat
    16321 tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca
    16381 attggttcag atctttaagc atatttatct tcctccttga ttgccttttt atcatattat
    16441 caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg
    16501 tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc
    16561 tatctatgtt ttacctttag aaaaataaat tttggggaag acagattcta agtaaacaaa
    16621 gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa
    16681 attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac
    16741 ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat
    16801 aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca
    16861 ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa
    16921 atatgtaaaa tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt
    16981 ttcatgaaag gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga
    17041 acgcggttac agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta
    17101 cttgtccgca acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa
    17161 gaccaacatg acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc
    17221 tgctgtctta ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga
    17281 actctcgtcc cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat
    17341 tcttatagaa catccgcact ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat
    17401 aaattattca gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa
    17461 caaaaacagg aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt
    17521 tgaatcgctt attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat
    17581 tgctactctc gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga
    17641 ggactgttat tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca
    17701 aatgttctct tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta
    17761 ctctataaaa atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa
    17821 aatagataaa aaactaaaga aacggtaaag aaagacgatt tcaatgaaaa atagcaagta
    17881 aacgataaca accatcctaa atccacaaga aatacagcag accttgcttt aaaaacacga
    17941 atcatgcaaa ggatgaagta aagaagactt aacgcgtacc caatattagt catttgacta
    18001 ttaggtacgc gttttgtttt ataagtcatg acaaatttcc ttcgttgctg gatagatttt
    18061 ccggcattgt tgatagattt tatgatactt ttctacattt tgtaagagag gttccgctgc
    18121 atctgtatat tggataaaat tctgacaaca actttcaggt gtgtcgaacc accctaaccc
    18181 tagtgcagca atcattgttg cgcctaatgc aggtccttcc tccacactta aacaatagac
    18241 cgttgcatcg aaaatatcag cttggatttg tagccatgca agatttttcg cgcctcctcc
    18301 caccgaaaca atacgtgtaa atctgcgacc cgctagttct ttacgtttca atcccaccat
    18361 tgacaaatgc tcattaaaaa tttagaatat tcacaagttt aacgacaatt atgaaaattt
    18421 atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa
    18481 agacacgtta cctaccgttt tcggttatat cggtattgga cttgcatttg gtatcgttgg
    18541 gaaagctgcc ggatttcatc cactagtcgt cacgttgatg tccctgctgg tctatgctgg
    18601 ttctgcccaa tttatcacag tcagcatgct tgctagtcac agcccattgc tttccatcgt
    18661 tttctcgacc tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta
    18721 tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag
    18781 tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat
    18841 gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact
    18901 tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat
    18961 gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca
    19021 gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag
    19081 caacttagtg attgtagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca
    19141 tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt
    19201 ctagggtcct accatttgtc ttactgaaaa aattcgattt accgcaaccg ttgcttgagt
    19261 atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc
    19321 aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac
    19381 ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggcagga attctttccc
    19441 tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg
    19501 cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt
    19561 gaacggacca ctaacaacga cttttcctaa catatgtcct gtttctacgt ctttggttgc
    19621 ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat
    19681 aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc
    19741 atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt
    19801 ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg
    19861 tcggacaagg tgcttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata
    19921 ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat
    19981 tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta
    20041 gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccggatcc
    20101 gttgatcact aagatttttt tcccgcgatt ggcattttct tctggagtta gaccaaattt
    20161 ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcag cttcttcatc
    20221 tgaaagattt tttggtgcaa gagcgacaat acgttcgtca actagttggt attcttgatt
    20281 gcttcctgct cttgttgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg
    20341 cacttcgtca cctacagcaa caactttccc gactccatca aaacctaaga tacgtaaagc
    20401 atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt
    20461 gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa
    20521 gctttttgga tcatcgatcg gcaacccttc ataaaaaccg actaccttca ttaattcctt
    20581 ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac agtgttcatt
    20641 tcaatatata tagtatgcta tttgttccac cttttttatt catcataatt attctcttac
    20701 ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca
    20761 caacctctta taaacggtgt tctcctacca actcttcggt cacaagtcaa aatattca
//
LOCUS       DACEMK010000048        20523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEMK010000048 DACEMK010000000
VERSION     DACEMK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.049"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW715_001548"
     CDS             <1..111
                     /locus_tag="IW715_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1022156.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(569..844)
                     /locus_tag="IW715_001549"
     CDS             complement(569..844)
                     /locus_tag="IW715_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022157.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1298..1504
                     /locus_tag="IW715_001550"
     CDS             1298..1504
                     /locus_tag="IW715_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022158.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1700..1867
                     /locus_tag="IW715_001551"
     CDS             1700..1867
                     /locus_tag="IW715_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1022159.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1893..2237
                     /locus_tag="IW715_001552"
     CDS             1893..2237
                     /locus_tag="IW715_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ1022160.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2242..2523
                     /locus_tag="IW715_001553"
     CDS             2242..2523
                     /locus_tag="IW715_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022161.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2626..2940
                     /locus_tag="IW715_001554"
     CDS             2626..2940
                     /locus_tag="IW715_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ1022162.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2918..4612
                     /locus_tag="IW715_001555"
     CDS             2918..4612
                     /locus_tag="IW715_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ1022163.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4632..5810
                     /locus_tag="IW715_001556"
     CDS             4632..5810
                     /locus_tag="IW715_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1022164.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5773..6459
                     /locus_tag="IW715_001557"
     CDS             5773..6459
                     /locus_tag="IW715_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ1022165.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6459..7619
                     /locus_tag="IW715_001558"
     CDS             6459..7619
                     /locus_tag="IW715_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ1022166.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7629..8504
                     /locus_tag="IW715_001559"
     CDS             7629..8504
                     /locus_tag="IW715_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022167.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8501..8812
                     /locus_tag="IW715_001560"
     CDS             8501..8812
                     /locus_tag="IW715_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022168.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8802..9155
                     /locus_tag="IW715_001561"
     CDS             8802..9155
                     /locus_tag="IW715_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ1022169.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9145..9546
                     /locus_tag="IW715_001562"
     CDS             9145..9546
                     /locus_tag="IW715_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022170.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9539..9943
                     /locus_tag="IW715_001563"
     CDS             9539..9943
                     /locus_tag="IW715_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022171.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9955..10563
                     /locus_tag="IW715_001564"
     CDS             9955..10563
                     /locus_tag="IW715_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1022172.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10583..10945
                     /locus_tag="IW715_001565"
     CDS             10583..10945
                     /locus_tag="IW715_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022173.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10948..11130
                     /locus_tag="IW715_001566"
     CDS             10948..11130
                     /locus_tag="IW715_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022174.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11147..14578
                     /locus_tag="IW715_001567"
     CDS             11147..14578
                     /locus_tag="IW715_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1022175.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14629..15366
                     /locus_tag="IW715_001568"
     CDS             14629..15366
                     /locus_tag="IW715_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1022176.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15376..17667
                     /locus_tag="IW715_001569"
     CDS             15376..17667
                     /locus_tag="IW715_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022177.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17691..19817
                     /locus_tag="IW715_001570"
     CDS             17691..19817
                     /locus_tag="IW715_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022178.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19980..20426
                     /locus_tag="IW715_001571"
     CDS             19980..20426
                     /locus_tag="IW715_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022179.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20428..>20523
                     /locus_tag="IW715_001572"
     CDS             20428..>20523
                     /locus_tag="IW715_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1022180.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         7229 a         3081 c         4291 g         5922 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcata taaacacgga aaaataattg cttataaata atttggcggt
      121 tttttggcgg aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa
      181 agattaggaa acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa
      241 acttcttctt agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc
      301 taaattcttc atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca
      361 tgttcttgtc gtctttcaac tgctgctgtc tattaattta tgtattggag ggaagaaaaa
      421 tgaaattatc agtagaaggc actccagaag aaatacaaga attgctccaa gctattggta
      481 gtagcaagga gcaaaaggta agtgtttcta attcagatat tgacggtatt tgcaattgtt
      541 taaacagaga aaaaatatct aatccttact aagagaaaat atgttcgtta atgaatttcc
      601 aattttcctc tgtagctcta ccttgataat ttttttgcag ttcacagatg aataaggaat
      661 cgttattatc aagtactgta tttaattttt cacacatttg atttggattc aaattagttt
      721 taactaacca aaaagatttt tgtagcttta tatatgcccc aaattctttt attatttcaa
      781 atactttatc atacttttgc cctgggttat taagatcata actaatcatg tagggtttat
      841 acatgttgtc ttgcacatcc ttgttttata tttcagcgga ccactcgctg ataaataaaa
      901 ttatacgctt agtatttatt ttcacaatat taatttatcg taataacttt tgagtaaata
      961 gaacaaaaaa cctgcactag tttccgctag tacaggcagt gactatatcg gttgataatc
     1021 tagcatatat taaaaataat tgcaagaaaa gaaatttata gtggtttata ccaaattatc
     1081 aaagcaatgt tattttgttg ctgtctattg ttttttaatt attcacacga taactaaagg
     1141 tgggtgaaga gaaatgattc cattaataat ttcaattttt gcgctctgtc ttaatgtcta
     1201 tatgattgga tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata
     1261 gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
     1321 ccatgcaagg ttcgattcct tgccagcgac attaaatgcc tatgacggtt acgactaccg
     1381 aaaaaagatc gttaagaagc tatacggtgc tacgtacggc aatgtagtaa gtgtgctatc
     1441 tgtacaccac caagcttcgg tcactgtggc ggaagtagaa gacgcagcgg taaatggcga
     1501 gtagcctcgt gagagcctgg taagttctcg tgagtggtgc aatcccactc cagcgacttt
     1561 aagcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg
     1621 tgcatcgtgc aagttactat tacatattag atcactcttt gagtggtctt tttatttttg
     1681 cacaaaggag gaaacaacaa tgtatagacc acaatactta gaacagaagt atgaagtaat
     1741 cactgttcat aaaggtaatg gcgaaaaagt atatgagtat agaagaccaa taaagagcga
     1801 tacatataaa cgaaaggaaa acaatgaagt tattccattg tatggcaaaa gaatagctaa
     1861 gcattaaata agattgcgaa aggagacgga acatgaccga ggaattctat agatggctat
     1921 tacagttgac aagagaagat cgtttggtta agttctatca gtctcctaaa tggcgcaggc
     1981 ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga agactaggta
     2041 agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga cctgacttag
     2101 ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa gttcatggca
     2161 gatatcttac agcattagat aaacaagaga agaagataga aagctttgct aacttcgatg
     2221 caagtgaaag gtggtaagtg catgatcatc aatgataatg gcagagagta tgatacagaa
     2281 aagattgaag agtattcatc ttatactcag ggattaatta aacgtttgat atacgttcgc
     2341 tatgtaggta ttagggatct gttatcagat aactgttgta gtaaatacaa agtgaatcaa
     2401 gtaagagaag cgttgaataa agataataac gtcgaaagaa taaaaaatgt ttttggatat
     2461 agtattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc gatggtgaga
     2521 taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc
     2581 aggaaaagag attttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa
     2641 ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt
     2701 cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa
     2761 acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa
     2821 aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat
     2881 aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa
     2941 gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca
     3001 attattgcta aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga
     3061 taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat
     3121 tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac
     3181 actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc
     3241 ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga
     3301 tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat
     3361 taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa
     3421 tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa
     3481 gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa
     3541 tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa
     3601 tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc
     3661 tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg
     3721 tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac
     3781 tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg
     3841 taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac
     3901 acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga
     3961 aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt
     4021 tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga
     4081 atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg
     4141 aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct
     4201 tgaaatgagt aataaatatt atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat
     4261 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
     4321 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
     4381 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
     4441 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
     4501 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
     4561 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
     4621 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
     4681 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
     4741 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
     4801 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
     4861 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
     4921 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
     4981 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
     5041 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
     5101 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
     5161 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
     5221 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     5281 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     5341 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     5401 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     5461 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     5521 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     5581 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     5641 gcattagaaa tggagtattt acaatcaacg aaggaagaga acgcgttgga aatgatcgct
     5701 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     5761 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     5821 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     5881 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     5941 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     6001 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     6061 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     6121 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     6181 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     6241 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     6301 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     6361 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     6421 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     6481 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     6541 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     6601 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     6661 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     6721 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     6781 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     6841 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     6901 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     6961 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     7021 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     7081 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     7141 ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata
     7201 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg
     7261 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     7321 gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     7381 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     7441 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     7501 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     7561 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     7621 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     7681 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     7741 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     7801 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     7861 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     7921 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     7981 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     8041 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     8101 acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa
     8161 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     8221 aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc
     8281 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     8341 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     8401 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     8461 gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     8521 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     8581 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     8641 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     8701 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     8761 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     8821 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
     8881 aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc
     8941 tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga
     9001 aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg
     9061 taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt
     9121 tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta
     9181 aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt
     9241 caagactccg taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca
     9301 ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact
     9361 ttctattcag aaaatagaga agatttaaca ggagactcac tagatattat ttcattgatt
     9421 caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa
     9481 gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat
     9541 ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga
     9601 acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc
     9661 tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg
     9721 gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat
     9781 cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc
     9841 taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat
     9901 tacagaactt ctgattcgtc aattcgataa attaaataaa tagggggaac aacaatggct
     9961 aaaacaaaaa ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat tgcttttaat
    10021 tttaagaacg caggaaatgc aatcgcaacg aattgtaacg gacaattaga tggcgaaaca
    10081 gaaatgcaaa cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa atctaaacca
    10141 atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgtttcaat
    10201 gggttgaaac aagatggacg tattaaacca ggcatttact cttacggtcc tgattccgta
    10261 ggcgaagatt tctcacttgc tgcagagatc gtggatgact tcgaagaaaa tagaaagtta
    10321 gttggtatgt tagcatgcac ttcgaataca ggattaacat tctctattga aaatggtgcg
    10381 gatgaagtag ctgcgttaga actagaaaca aaagttatgc aagatgaatt tggtaaattt
    10441 tatcatgaag caattgttgc agaacttgaa gaagacttaa cagatcaatg gatgacgaat
    10501 ctatctgctg atgtgattaa aaaaacttca gttgtgacaa ctacggccac tcaatcacag
    10561 taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac taaacgatgg
    10621 aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga tcaatagaga
    10681 ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg atatttctgt
    10741 tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga ctgattatat
    10801 ttcattcgat gaatttatcg ataaatggga ttttgatatg gctactgcca gctatattta
    10861 tcaattgatg atgttcaaac aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa
    10921 taaggaaaaa aagcttcaaa agtaaaaatt ccaaagctct tagttgaaac gtgggtcgat
    10981 gtctattcga tgttgaccga cgttttttct atgccttcag atttggtttt aagcgatatc
    11041 tgtttagatg acattttgca aatggcttac aacaagagtg cttatgaagg atggaaaaac
    11101 tatgcaataa accaatccca gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag
    11161 aacagaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga
    11221 aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca
    11281 acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag aaaagcagta
    11341 tgaattacaa agtcaaaaag ttgaagtaac tagccaacgt ttagccaatg cgaaaaaata
    11401 ttatggagaa aattccaccg aagttaagaa acttgaaaga gaactgatta atcaacaaac
    11461 agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag ctcaagcaaa
    11521 aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagcgaac aaaaaaatgt
    11581 tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagcaa ctgctggtca
    11641 atcagcttct gaagccgaga aattagcgaa agcccaagaa tatgtttctc aacaatctga
    11701 aaatgcggag aaaacgatag atatcctaag acgacagtta gaagctacac agtctgaatt
    11761 tggcgctaca tccacagaag caatgcagat ggaagcgaag cttaatgatg ctgaacgtga
    11821 atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg acgatatcgg
    11881 aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg acattggcga
    11941 taagcttaca gaattaggga aacaagcagt ggactctgct aacagtgtag gtagttccca
    12001 gagtaagata caagctaatt ttggtttgtc taaacaagag gctgaagaat taacgaatgt
    12061 agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca cagatgcatt
    12121 gattttggta aagcgtaatt taggagattt aaataatcaa gatttacaaa atatcacgga
    12181 acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt tacgtggtgt
    12241 aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt taatggtttc
    12301 gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg cagaatatag
    12361 ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg
    12421 tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg aaatgggaat
    12481 atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa gtactagaaa
    12541 agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta attccatgat
    12601 tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt cgacaatttg
    12661 gtctgcgctt ggcgaagata atgcgatgaa agttgtccaa tctttaactg atgttaacca
    12721 tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta ctactccgtt
    12781 gcaagagttg aacgggaaaa tagctgaatt aaaggattca ttagctccta taggcaacac
    12841 aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg atgcgtttaa
    12901 taatcttcca caaccagtac aggatttcat tgttgctata ggtggtctta cagcagcatt
    12961 tggcatactg ttaccggcaa tactagccgt gtcatattta tttggtccga tgatgcttat
    13021 aattggagga attatagctg ttatagcagg cgttattgtg gcaattaaga actggggtgc
    13081 aattactgac tggtttagtg gtttatggaa aaaatttact gattggttgg gtgatacttg
    13141 ggaaagtata aaagacggag cctcatcagt ttgggatggg gttaaagaaa cctggtctgg
    13201 atttgtagat tgggttcaag atatttggca aggagtttct gattggtttg gagagctatg
    13261 gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaaa cttggcaaac
    13321 attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg
    13381 ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc
    13441 tggtattaat attatcgtgg gtatttttga tgtagctgga cagttattgg gcgcagcttg
    13501 gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata ctatgacaca
    13561 atggataagt atcgcttgga atgggattgt aactacattc catactatat ttgatccagt
    13621 agtggcatgg tggaatggta tatggacagc tattagtact acggcttcaa atatttggaa
    13681 ttcaattagt gcaacagctt ctagtatttg gaacagtatc aagaatacaa tcactagctt
    13741 ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg
    13801 tggaatttgg aattcaatca gctctacagc atcaaatatc tggaatagtg tgactagtag
    13861 tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc
    13921 ttcgtggatt agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg
    13981 aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg
    14041 gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa
    14101 catatgggaa ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat
    14161 cagtggcatt tttgataatt tgaatatttc tataccacat attccgttac cggaatttat
    14221 catggaaggc agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca
    14281 tgctaaagga ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga
    14341 agcaggacct gaagcagttt tacctttgaa aagatctgtt ttgcaagaaa ttggtgatcg
    14401 tatcttgagt agcacatcag tttcatctag ggcacaaacg attcaacctg tgaataacta
    14461 cgaattcaat ttcacaattg atggtaacgc agatgaggtt actatgaagc aaacaactca
    14521 acaaatcatt gatagcatta caaaaattca aaatgataat gcttcggcat ggcgttaaac
    14581 aggagagtat ttctcctgtt tttttagtat taaaaaggat gtgaaaaaat gactgattgt
    14641 atacattcta taatcgatgg atttcctgat tatttgcata aattggcttt agctgaaaga
    14701 ccaaccatac cttctccaaa aagacagaga gttgaaactt ctgttttagg taggttaggt
    14761 ggcttagtac aagattactc gtttgaagat atgtcgttta cattgcacta taactattta
    14821 gaggatgtgg aagaccatca agcgttcaag caatcgtttt atatcatgcg tcattggtta
    14881 aactatgcaa agaaattaga attctctgac gatcccaacg tctattacgt tatccagact
    14941 atcgatattg gggatgcaga aaacgatatt gttgaatggg gagagttcga tgtaaatatt
    15001 actgcgaaac cattcgcaag agttcaagaa gatgtaccta taaccgtaga taaaccacag
    15061 tcatttaact tgctgaataa tagtttagaa gaaagttttc caaagattat catcactcct
    15121 tcagctactt catgccagtt catcttaaat gattatgtgt ttagtttcga aggcttagta
    15181 gcaggaactg atgtagtcat tgatagtgat ttgatgcttt gctacgaaga gcaatcggac
    15241 ggagatattt tagatcggtc caacaaaatg aagaccatgc aatatccgac attgcaagtg
    15301 gatatcaatc attttaattg cactggtttg agcaaaatac aaatttatcg taacgggtta
    15361 aggtaggtga aatagatgat cgataattta ataactattt acgataaaaa tgacgcgaat
    15421 aatttagctg aacatttata tgatacgcaa ggtttaggcg ctttgtcaga ctggttaaca
    15481 gctactgtta gcaataaact aaacggagcc gagatatttc agggtactta ttcaataaac
    15541 ggaactaatg cagatttgat tgtagaagga cgtattattc agtgttatgt agatgaaaat
    15601 cgagcaaaac agcgtctacg gatttattat gcaaaaactt ctgtaatagg caatacgata
    15661 gaagtaaaag ctgaacctat tttcaatgat ataagaaaat cggtgttgaa taaatatgac
    15721 agcggaacag aaaagatcac tgctactcag gcatggcaaa acgcaaaagt tttagcgaaa
    15781 ccagctatcc cttcgcagtt ttctttctcg tcattagtag atacgcttgc taatgtgaag
    15841 atagaaaagg cgaatttttt agaattcttt ggtggaaaag agggatctat tctagatcga
    15901 tttcatggtg agtttctaaa agataataac acattacgtc atgaaaaaag tctaggtacg
    15961 gatcataaaa tcaaagcgat ttatactaaa aacttaactg gtcttgactt agagattgat
    16021 gctcaaagtg ttttagttgg agtttatcca ttcattagca gctcttcaga aggagaagac
    16081 gagatcactc taccagaaga agttattttc acggattacg tggatgatta tcctgctgga
    16141 tatgtttctt ttgttgattt taaagacaaa gcgactgatg tagccacatt aagggaagct
    16201 gctaaagact ggttgaaaac aaacatagat aaacaaaaac cacaagtgag tggttcgatt
    16261 gaattagtac cattgaggca tcaaagaggc tatgaaaaat ttgttgatct agaaaaagtt
    16321 tcgatgggtg acggagtaga tgtgtatcat ccacagttaa aagtgaatat gtcagcgaga
    16381 attgtggaat atacgtttaa tgttctaacc aattcatacg ataaattagt tgtaggaaac
    16441 gtcaaaacaa acttcttaga aaacacagag aataatgtca gcaatttgat taatgatgcc
    16501 attgatcaat tgaaaaacgg tggcgaaatc agtgatttac tcaatgatat tgtagatcat
    16561 caaactgata tgattactgg tcaaaatggt ggttatgttt tattagatcc taaagaagcg
    16621 cctagtcgta ttttgattat ggacacacca gataagaata ccgcaaggaa tgttttacaa
    16681 atcaacaatg ctggtattgg tttttctaaa actggcatta atggaacata tgaaacggca
    16741 tggacgttag atggcggatt caatgcctcg tttattacgg ctggtgaaat agtaggaatt
    16801 actattagag gtactacatt aattagtgat ggtgctgatt atagaacaag tattgctaat
    16861 ggcaaaatga cttggtactc aaaaaaagtt aacaaagata ttatggagct agaagcacgt
    16921 gattatgtaa gtgctgatgc cggtattgta tcatacacca tgaaaactgg tggtggtttc
    16981 atgattagaa atccacaggg taacttggtt tttagtacgt gggataatgg taataacaga
    17041 ccgtttctat cttttggtgc gcccaatttc aggtatagca atgctagtta tgtaacttct
    17101 ggcgacggta gttctttaag cattaatggt agtgcgggta actcatggga atttaaggta
    17161 gctggtagga ctatgaaatt tactagtgat ggtatgctaa cgttaccagg ttgttttttt
    17221 ggttcatggg aagatgggaa acttgctagg tttgaacaat caacggtaca agtatataaa
    17281 gattttactg ttagaggtac taaaaactca actgtaccaa cagaacatta tggacaacga
    17341 ctattgaacg cttatgaaac tccagaatat tatttcgctg attatgggga agccgttaca
    17401 ggtgacaatg gtaaagttcg tgttgatatt gaccccatgt ttgctgaaac agtaaatcta
    17461 agtcggtata tgacacatgt gacacctaca gaactagttt tgtgtgctgt tactcatgaa
    17521 gatattgacc atttcatcat tgaaactagt aagccaaacg tattagttag atggaattta
    17581 gtggcacacc gtctagggta tgaagatatt agattaaaag aggatacagc atatgatagc
    17641 acagtgcttg accaaaaacg tttttaaaac gaagacaagg aggtatataa atggctagca
    17701 gtttatataa tttggcttta gatttcagca aagaattaaa ctacaccaaa gctattatgg
    17761 ctcgtcaagg tgataaaggg attacggtga cggttaaacc gtttctaaat ggcttgcaga
    17821 tggatacgag tggcggaaca tttactttaa aaggaacaac accatctaac cgttacgtag
    17881 ataatgttgc aactagtgta actagtgaag aagtcacgtt ttctcttgat ggcacattta
    17941 tgagtgaagc aggatattat aaacactgct acgtagaata tagaaaagac aatcaaattt
    18001 taacaacgca agatatcatt tttttctcac taggagtgtc tgacatttcg caaggccaag
    18061 ccgatgaata tgtttcgcaa ttagaagagt tgattcgaaa gtacaacgaa acttttgatg
    18121 cttttatggc tgaaatcaaa ggtagagtgg atagcttaaa tcaacagatt actgatttaa
    18181 ctggtcaagc taaaacgcta caagacaagt tagatgctct gaaagaagaa atttctaagt
    18241 taggtaactt acaagtgatg tacagtaaca gcatcgactt cggggactat gattattcgg
    18301 agaacccgaa tttaatgcca tatattacag aaccttgggt tggacctttg ttaggtaatg
    18361 gacatacagt taaagatagt gttaaaaggg ttataactca tactaaaact agaacagcta
    18421 attccggcga catattatcg cttggcttag gtattccgtg tacagcggaa gctaataata
    18481 ggtatcttat aaccacttta cgaccatcta cgacatacac attaagcgtt actatgagtg
    18541 tcggttcaga ttggactgga gagaccaata ctataggggt taggcttaga tatttaaatg
    18601 aacaaggtgg tatagaactg cccattaatg cattaatacc agcaaatgta gaacgcgata
    18661 aaatggtcac tcatacattt actggtataa caaaagataa tgtaactagc ataacaaact
    18721 gttatgttga aatattctca cttaatagtg aatataaagg aacggttagt gtcagctatg
    18781 acgttaagtt aaaagcacac taccctaact tactagatgg cccttactgg ttaggtaaag
    18841 ttcctttagg cgagaatatt gctgacccta cagttgtctt tccacacaaa actagtgaat
    18901 atatggttta tggtagacgc aatacagaaa actatatcgc tgaccaaaca tataccatat
    18961 caatgaaagc aactaaacta acagttcaat cgtttgccgt gtatattgct gctggccgcg
    19021 ttaaagtagg agacatgaag cctactgagg ggttagctaa tacatgggag ttaactttta
    19081 ctgtaactaa gcaacatata gatagtgggg taactaatta tctggaaata taccaatacc
    19141 cgtcagctac aaaaggagct gttcaaattg agtggctcaa actagaaaaa ggcaatactc
    19201 gaaccccgaa tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc
    19261 caaaagatta tgtttgggat ctagcaccag aatatgtcga agataatttg gccacagata
    19321 ttaaaatttc tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcggaga
    19381 ttaattcgca gttgactgct tcaattaatg aagtagacac cacagctaag gatgctcaaa
    19441 caaaagcgaa tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca
    19501 ttaatgatac agctactacc acattaacag ttacaaacgg gaataccgga tcagcaaagc
    19561 tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga aaaagcagtg
    19621 gtggaaatga ttcaacgata ctaacaattc cagaaggcta tcggccacca attagttttg
    19681 agcaactggt tggctcgata gaccgttcta ctttgaacag tgctcagtta tctattggtg
    19741 cagatggagc cattaaatgg cgaagaaact caagttatgg atcggattat acctttgcaa
    19801 ttacttacac gatttagaaa agcgtgaatc gatatgaagg cagcatatag accaattgaa
    19861 ccttacggat tcgagcaaat cattgtgaat gatgaagaac atttaccgga agaatgcaca
    19921 gaagtcgaac caccgattcc aaattggaaa ccgaaattca attactggga gggaaataaa
    19981 tgaaaaacat ttggaaatat ggacgtactg gcggagagta cgcaggaaaa gtattggacg
    20041 acatgcttgt atccgttcct tacacagatc agccaccgct tgaaggaatt cgtgctgatg
    20101 gcgaaccgtt aacaatcgct gatcaaatgt ttgatcctaa attgaatcaa tggattattt
    20161 tagcgaacgc actagatcac aacgatttaa acaatctcaa agcaatgtat gagtcgttag
    20221 aaaatgagaa cggcgattta aaacagatca atgccaaact catgctaagc gatgtagcaa
    20281 ttaaacagga aaatactgca ttgaaagaaa aagcggatag tttagcacaa atcaattcaa
    20341 aaatgatgct tgcttcgtta caaaatagca aagacatttc agaaattaaa gagcaactaa
    20401 atccagcttc aaagggaggt gagtagtatg tttagtttta gcgatgtgaa aatgatgtat
    20461 gattggggct gtttcactaa cgaacaagta atggttttcg ttccgttgtg cattactgaa
    20521 gaa
//
LOCUS       DACEMK010000049        19850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEMK010000049 DACEMK010000000
VERSION     DACEMK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.050"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..111
                     /locus_tag="IW715_001573"
     CDS             <1..111
                     /locus_tag="IW715_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1022181.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="IW715_001574"
     CDS             124..510
                     /locus_tag="IW715_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022182.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1061..1285
                     /locus_tag="IW715_001575"
     CDS             1061..1285
                     /locus_tag="IW715_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022183.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1303..1731
                     /locus_tag="IW715_001576"
     CDS             1303..1731
                     /locus_tag="IW715_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022184.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1709..3127
                     /gene="terL"
                     /locus_tag="IW715_001577"
     CDS             1709..3127
                     /gene="terL"
                     /locus_tag="IW715_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1022185.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3139..4674
                     /locus_tag="IW715_001578"
     CDS             3139..4674
                     /locus_tag="IW715_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1022186.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4754..5632
                     /locus_tag="IW715_001579"
     CDS             4754..5632
                     /locus_tag="IW715_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1022187.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5634..5951
                     /locus_tag="IW715_001580"
     CDS             5634..5951
                     /locus_tag="IW715_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022188.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6000..6701
                     /locus_tag="IW715_001581"
     CDS             6000..6701
                     /locus_tag="IW715_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1022189.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6716..7606
                     /locus_tag="IW715_001582"
     CDS             6716..7606
                     /locus_tag="IW715_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1022190.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7629..7844
                     /locus_tag="IW715_001583"
     CDS             7629..7844
                     /locus_tag="IW715_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022191.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7856..8197
                     /locus_tag="IW715_001584"
     CDS             7856..8197
                     /locus_tag="IW715_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022192.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8197..8568
                     /locus_tag="IW715_001585"
     CDS             8197..8568
                     /locus_tag="IW715_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022193.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8561..8956
                     /locus_tag="IW715_001586"
     CDS             8561..8956
                     /locus_tag="IW715_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1022194.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8958..9335
                     /locus_tag="IW715_001587"
     CDS             8958..9335
                     /locus_tag="IW715_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022195.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9336..9944
                     /locus_tag="IW715_001588"
     CDS             9336..9944
                     /locus_tag="IW715_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1022196.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9944..10285
                     /locus_tag="IW715_001589"
     CDS             9944..10285
                     /locus_tag="IW715_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022197.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10527..13316
                     /locus_tag="IW715_001590"
     CDS             10527..13316
                     /locus_tag="IW715_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1022198.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13306..14046
                     /locus_tag="IW715_001591"
     CDS             13306..14046
                     /locus_tag="IW715_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1022199.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14043..16805
                     /locus_tag="IW715_001592"
     CDS             14043..16805
                     /locus_tag="IW715_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ1022200.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            16818..17726
                     /locus_tag="IW715_001593"
     CDS             16818..17726
                     /locus_tag="IW715_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1022201.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            17726..18343
                     /locus_tag="IW715_001594"
     CDS             17726..18343
                     /locus_tag="IW715_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022202.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            18347..18796
                     /locus_tag="IW715_001595"
     CDS             18347..18796
                     /locus_tag="IW715_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022203.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLTNITFEKVGTV"
     gene            18796..19320
                     /locus_tag="IW715_001596"
     CDS             18796..19320
                     /locus_tag="IW715_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022204.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
     gene            19348..19755
                     /locus_tag="IW715_001597"
     CDS             19348..19755
                     /locus_tag="IW715_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022205.1"
                     /translation="MKTIYKVLYPMGYEEHQVEDNFPTSLPFVEVPPILFEKKEDETD
                     EGFGRRQQSQFFNFTENKWEEAVTQDYSKKLELLENLSIGLQVDNAALKKSNEELTEK
                     ADSMAQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            19755..>19850
                     /locus_tag="IW715_001598"
     CDS             19755..>19850
                     /locus_tag="IW715_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1022206.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDE"
BASE COUNT         7089 a         2788 c         4213 g         5760 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
      601 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
      661 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
      721 attatctatc atccgttgct gtctattgtt acatacagat cgcttaggcg gtctttttat
      781 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
      841 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
      901 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
      961 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
     1021 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
     1081 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
     1141 tgttcaaatg aaggcgaagt attcgattgt agaaatgatc agagaggcaa cgccagtaga
     1201 gattgattat tgcaagctag tctattgtgg gtgcggccgt tggaaagagg atcatgtaca
     1261 aaaaaatatc agcaagtaca tatagagagg cggtgattaa ggatggaaaa aagcgatacg
     1321 aaagcgaaca gatacaaacc tactgctgca gagaaaaaac tcttggaagt attgataaat
     1381 ccagataact taggcaaacc ggtgcaagag ttgtgttcac tggcgaaaat aggtaggacc
     1441 aaatattacg acgccatgag caaagatggc tttgttgatc tagttaacga aacaacaatg
     1501 gatctgatca aaggaaaagc ttctgatgtc cttaatgcca cttacaagta tgcattgact
     1561 gaaaaaggtc atcaggatag gaaaatgatt ctgactattg ctggaatcta tgcagacaaa
     1621 caagaactca aacattctgg cggcgtcgat atccgaaaac agtatgagaa aatgtccgat
     1681 gaagaattag aagagttggt gaaacggtat gaaaaaatca acgactctta atcgtgttga
     1741 gtatgccgtt gttcaaagag agctagccat tcgaaaagct aggaaaagtt tttggctatt
     1801 ctgccagctt ctttatccag agttttacaa agatgatcgg ttgtatttga aagatttatg
     1861 tcatacgcta caagattttt acgaagataa aatcgacaaa aaaattttgg ttattaacat
     1921 gccgcctcga catgggaaga cttttacagc gcggctattc gttttatgga tgtttggaca
     1981 aaatccaaag acgaaaatta ttaccggatc ttataaccaa atcctgtcat ctttatttgc
     2041 gcaacaaaca cgtgatggaa tcatggttga aaacgaaaat gttaaggctg aatactttaa
     2101 agatattttt ccagaggtgc aaatcaaata tggagatgct gcaaagggtt tctggtcttt
     2161 agatggttca gaagaaaaga attatttagc gacttctcct ggtggaacat ccactggtat
     2221 tggcgcgaat tatgtaattg ttgatgacat cattaaaacc gcagaagaag cctataacga
     2281 gcgtgtttta gattctcact gggaatggta taacaacacg ttggcccaac gaatggaacg
     2341 gccacgtaaa cagatattga ttatgacacg ttggtcaagt aatgatttag ccggaaaaat
     2401 gttgacgaga cgaaaaaata atgttcatca gatctgttat aaagcagtac aagaagacgg
     2461 ctcgatgttg tgcaacgaga ttatgaccca cgatgagtac ttagatgtgg tacaagaaat
     2521 gaatgtagat attgcagaag ccaactatca gcaagaacca attgaccaaa aaggcaggct
     2581 atatcagaag tttcttactt atgatacgtt gcctgataac attatcaaaa tatggaacta
     2641 caccgacacc gccgacaagg gtgccgatta ctttgcgtct cctgtttttg ctgagacgtc
     2701 tgatcatgat gcttatatta tcgatgtcat gtatacaaaa gagccgatgg aaacaactga
     2761 aatagcccac gctaatatga ttattcgcaa tcgagttaat aaggtacgga ttgaaggaaa
     2821 caatggcggg cgtggattca gaagaaattc agaaagaatt acaaaagagc gaggctatcg
     2881 aggtgcagta tttgaagact ttcatcaatc ggataataaa ctgtctagaa tactggcgaa
     2941 tagtgcttgg gtggaaaatc atgtttacta tccagatgat tgggcgacac gttggccgga
     3001 cttttatgag gctatggtta gttatcaacg tgaaggtaag aacgctcatg atgatgctcc
     3061 ggatgcagtt acaggaattg cagaaacgat tatagaagca gatgacttcg agtttattgc
     3121 ttattaggag gtgggtcttt gttttattct ggaatcacat tgaaaaaata caaacgttta
     3181 agaacgaaat attcaacgca aatcgaagaa ggattattcg atcctaacgc ttttatggaa
     3241 gatatgaaac cctttttcga tgatcgagaa agaaagtatc ttgcttatac tagcgagaaa
     3301 aacgagatcg acagtcgtga gaaaccaaaa acaaaaatca ttaaggtcaa taacaagatt
     3361 catgcgggaa tgtattcagt ggtcgttgat caagcagtta accatttcac aggaatacca
     3421 attaaatggg attatgatgt ttcggaacag aaaaggacat tcattcaacg aatgagagac
     3481 aaattactgg gtaatgatat cagcgcccct tctaccccag aagaatttga aacactcacg
     3541 agacaattaa gtactatgcg gtttggaatg ttagaccccg atactgcaac atttcaagga
     3601 gcgacagggc ttgctttcag attactggag cctgttgaga caaatgatgg ttgggaacta
     3661 agagcaagca acattgagcc gtggaaagct gaaaagtacg aaaacggcgc agtgtacatt
     3721 aaagagaaat acgatccata tcaaaagaag ttttatgaag agatgaaggt tgtcacgccg
     3781 aaatcaatat ttacttatgc tcgatatact gattaccctg gcacggtaca aggagcattt
     3841 aaaacgctgg gtaatgttcc taatccttta ggcgttatca atcttacgga gtttaaaaac
     3901 aatacaaacc gttactgtga ctttgaagtt gcagaggaga ttagtgatgc tattgatcgt
     3961 gttttatctg atcagcagaa cgagattgaa caattcaagt tagcttatat gctgatcact
     4021 ggggcagatt tgtccgagga taaagccaag aagatgatgg agcaactagg gatcatcaat
     4081 ctgaaagacc ctaatgcgaa agccggctac gtgacaaagg atctacaaaa ggatttcaac
     4141 gagtatcatt ttgatcttct taaaaagcaa ttctacacgg tgtgtaaagc aattgatttc
     4201 aatgacgaag tctttaagtc gaatagttct ggggaagccc gaaagtggca aatcattagt
     4261 ttagaagcta agacaaatac caaagaacag tactttcgag aaggattgaa agagtgtgcc
     4321 gaggttatgg ctgcttattt ggattttgcc gagaaagtta caattgatcc ggaaaagatc
     4381 attttcacct tctctcgttc gcttccaaca gatttaagct acctagctga ggcattgcct
     4441 aagttggcac catacgtttc taagaggacg attcaaagtc agatcccgtt cgtcacagat
     4501 gttgattatg aaaacgaaat gattgacgtc gaaagcagac aatcttaccc agacggcgag
     4561 tatggaggtg gcatcgttga gtcaaataga aacgaagtac tggaccaaac gtcgagaact
     4621 ggaagattta gcaagactca aaatggaaaa tcaaacgcta aagaaactaa gtagtgtctt
     4681 tcctgatgcg ttgaaagaaa ttcaagcgga gttattatcc caagctgatt tacacgatat
     4741 tacctatgct gagatgatgg aattttatag tacaagtgat cagaaaaaat atcgtgaata
     4801 cgttgaaaag aactacaaat cactgaagat gtatgatgca aaatacaaag agttcatcga
     4861 agaatacttt ccttctttcg actacgccaa agtgaatcgg ctgcttcaaa tgaggtcaga
     4921 tatttttcga attcttgcag agagtgcaat agatgcagat gtgaataaat atttttcgga
     4981 tcggttagaa gatatcttgc aacgttcgta tgtatcaaac gcaaacgtat ttgcccagat
     5041 tttgaacgta gatttgccga attacttatc aaaggaagaa cttgaacgtt acctgaacta
     5101 tccttggtca ggtaagacat tctcgcgtcg tttatggggc aatatttcat ctctagaaca
     5161 aaagctatct aacgccatta caactagtgt agcaagtggc gaaggagttc tcgtggcatt
     5221 acgaacaatg cgagaagacc cagaaatatg cgatatgttc aaaagggagg aatcgaagta
     5281 caacaaggcg atcgaaaatc ttgtgagaac agaatacgct aaattcgctc aagatggtat
     5341 tgagaaatct tatattgaaa ctggcgtaga cgaatacaat gtcttaactg caaaagatga
     5401 aaaggtatgt tcaatatgcg gtggcaaagc aaagaacaac ccatacaagt tgagtgaagc
     5461 gatcattggt gagaaccgtg cgccttttca tggccgatgc agatgtacag atgttcctaa
     5521 tatgccgaaa ttaggaaaag atattgacga agagtatgag cgtttatttg gcgatttatt
     5581 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
     5641 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
     5701 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
     5761 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
     5821 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
     5881 ttgattggta tgaaaatatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
     5941 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
     6001 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
     6061 cgtggggcga aaggagaatg attatgaaac acaaatcatt aatgccaatg aatttgcaat
     6121 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
     6181 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
     6241 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
     6301 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
     6361 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
     6421 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
     6481 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
     6541 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
     6601 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
     6661 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
     6721 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
     6781 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
     6841 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
     6901 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
     6961 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
     7021 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
     7081 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
     7141 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
     7201 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
     7261 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
     7321 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
     7381 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
     7441 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
     7501 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
     7561 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
     7621 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
     7681 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
     7741 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
     7801 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
     7861 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
     7921 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
     7981 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
     8041 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
     8101 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
     8161 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
     8221 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
     8281 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
     8341 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
     8401 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
     8461 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
     8521 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
     8581 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
     8641 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
     8701 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     8761 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
     8821 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     8881 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     8941 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     9001 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     9061 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     9121 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
     9181 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     9241 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     9301 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     9361 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaatttcc
     9421 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     9481 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     9541 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     9601 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     9661 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     9721 gacagtttgc tctagattca gatgaaacgt caacgattga aggaagtaaa gtaagtttta
     9781 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     9841 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     9901 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     9961 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
    10021 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
    10081 aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
    10141 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
    10201 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
    10261 ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt taagtattcg
    10321 attacttacc gagaattaga tataaagacg caattactcg aagcaggttg gacgttacca
    10381 gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag agatgcggta
    10441 aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga ggtgatactt
    10501 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
    10561 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
    10621 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
    10681 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
    10741 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
    10801 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
    10861 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
    10921 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
    10981 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
    11041 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
    11101 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggtttg tctcaaggta
    11161 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
    11221 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
    11281 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
    11341 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
    11401 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
    11461 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
    11521 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
    11581 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
    11641 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
    11701 tagctgtttt cggttacttt atggcaacca atgaagagtt tagaaacaag gttatatcta
    11761 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
    11821 ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt gtcaaagatg
    11881 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
    11941 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
    12001 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
    12061 gagaaaaatg gaatgaaatt aaaggttcta ttatgtcggt tgctggtcca ttgattacag
    12121 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
    12181 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
    12241 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
    12301 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
    12361 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
    12421 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
    12481 atttttttat taatctttgg attgatataa aatattttgc aattcagaaa tggattgaat
    12541 tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt actacttgga
    12601 ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg gcatacaata
    12661 cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag gactctttct
    12721 ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat ctaaaaaatg
    12781 gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg gaagtcaatt
    12841 tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa gaaaagtgga
    12901 atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg attaaaggag
    12961 ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa aatattgttc
    13021 aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc atgacaaatt
    13081 tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta ccaattacac
    13141 aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat ttaaatgttt
    13201 atggagattt acctgattca atggttcgga agattaccac aaaaattaaa acagaattga
    13261 caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca acttaagaga
    13321 ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat tcaaaacagg
    13381 cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg aaatgattct
    13441 atcattattg ataaaggcta ttacagaaat gtgactagaa agattgagtg ttactataaa
    13501 gcaccttcaa ttgatgtggt gcaggagtgg gaagatcgaa tcactgaatg gttagatatg
    13561 tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca agctgtagta
    13621 atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc ttttgaattt
    13681 acagtgagca ttcgaccatt taaggaaaat tacaatggta gatttactat tcgacaaaca
    13741 gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat taaattgagt
    13801 ggttctggag atgcttcttt ttatattaat aaggacaaat atgatttgaa atcgttggac
    13861 agagaattat ttatagattc taaattagaa gaggcttacc gaaaattaga tggtaattta
    13921 gaacatcagg atcaagtcac tttattttta gattttccat ttttatatcc tggaaaaaat
    13981 gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg gtggagaaaa
    14041 gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat ggcttaggaa
    14101 ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa tatgaattag
    14161 agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac tatcaaataa
    14221 aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat aaagatatag
    14281 atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga cttggaagac
    14341 gggtagtcac tcttgcggaa gtagactcta aaactggtca agaagcgata tcaattattg
    14401 aaaccaaaat ggataaaaaa tctgacatac gactttattc tgatattacg gccgtttcta
    14461 gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa ggatcattgc
    14521 ttcaatattg gggtggagaa attaaacgtg aaccatttaa gctttctttg ttaaagcgaa
    14581 gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt ttaaaggtca
    14641 aattagattg gacaggtata aaaacaagac ttattccata tgctgatcct caaagtgagg
    14701 taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt aataactatc
    14761 ctgatgtgta tacagagcat gttcagttta cagaagaaca aggagtaaaa gacattaaga
    14821 gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt gataagccta
    14881 aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg aaagaatttg
    14941 cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa aaatataata
    15001 tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa aaaactttga
    15061 aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta actattcagg
    15121 ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat tatgtttctt
    15181 caatgattac aggtcaaggc agtgcaggag gatatgtaag tttatggcca aaagaaaaac
    15241 cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa gtactaagaa
    15301 tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt acttcagctt
    15361 ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc aaagcagata
    15421 tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat ggactactcc
    15481 aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg gaattttgga
    15541 attctaaggg ttcaattgga acgattggaa caactgattc tgctggtaat ccttttcctg
    15601 gggcttctac tccaacccct attgaagata attctttagt tattcgtaca aatggagacg
    15661 gcaaatatat tttgatttct cctaaagtag gaaaaggatt agttttatta ggaaatggta
    15721 aagcaattta ttttggagac ttagatgtac aaggaaaact tacagttaaa ggaaaagaaa
    15781 taattcctgg acaaggtggt ggtccatcgg gaggtggaga atctactggt gggtatccta
    15841 gtgaattaaa aacagatgca gaaaagagag cttggagaat ttacgatatt ttgtgtaata
    15901 acggatttac gaaacaatct gcatgtggaa tattggggaa cattcaacaa gaaacaggag
    15961 gaacttttga tcctgatact gttcaaatag gtggaccagc atacggatta gttcagtggg
    16021 atggttcttc atatcctctt gttggtccag caacatggga tggaaaagtt tatgttcaaa
    16081 acttatttaa tgctgcaggt attaaagaac caataacgag cttagatgca caagttcgat
    16141 tgcttatttg gacatttaca aatggacaat ggatgggcgt agtacaacct acgacggttg
    16201 atggatttaa ggcctgtact gatccaagac aagcagcata tgcttttgaa cgaaactatg
    16261 aacgtccggc agcgacacat cctgaacgtc aagattatgc agttaactgg tataacaaat
    16321 ttaaagattt aaaaccagga ggagctactg gagaagcagg actaaaacat ttagagtctt
    16381 tgattggaca acgaattggc aatggtcagt gttatggctt gtctgcagaa tattcaggat
    16441 atctgggtgg ttgtggcatg ggtgctggaa caaaatatgg tttaactcat gtgataggaa
    16501 atacttctgc agcatctgat attggtattg cctatgattg gtctgctgtt ggttggaaag
    16561 tgattcaaaa ccctagatat gatcaattag tagttggtgc aattattaat tgggcaagag
    16621 gtggacaagt gggttcatgg tttgcagacg gaacttatgg acatactggc gttatccgag
    16681 gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt atgatttgtg
    16741 gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc ataccaccaa
    16801 aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca gaaccctata
    16861 attacgtggg gatgattcaa gttcgacaag gcaataaaaa cactgaagtt atggaagcta
    16921 caattgtcga aaatggtctt ccatatgatt tatcagaatg taaggtatat tttgaatcag
    16981 ttgttggcgg aaaatatcca gtccaattag aaacaagaat tgtggatgct aaaaaaggga
    17041 aaatcaaata tatttttgat aaatattcca tgcagtgttt gcatcgacaa acagccaatt
    17101 tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc tattttgtca
    17161 tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca attgaagatt
    17221 taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg gactggatga
    17281 atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt ttccaagatt
    17341 ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag gatattttga
    17401 agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt gttgatattc
    17461 aaggagttcg tcatgcgtct atttcagagc gcttgctggc agatatggat tatctatatc
    17521 agaaattacg agcaacactt ttcacgattg aatacggtga gattgaagtg actgatattt
    17581 tgcaggatga tctcttttca gataatcacg aagtcgaaaa agttggaact gtagaatttc
    17641 caattgaaga aggagctttg attattgcaa ctgttgatga tccaaaacag aatgttttca
    17701 ctcttgagaa agttggggtg atttgatggt taaagtaaag agaatgatgg agactgatga
    17761 atctggtgtt aaacgtcaat ttatgccaat tacacatgtt tctgcagttt taggcttaga
    17821 aaaaatgata tcaggacaat caaaagttct ttcggttaat ggtaaatatg gtgcagtcat
    17881 tttaacgaaa gctgatttag gattagagaa tgcaattact gaactacctt atgcaagcga
    17941 gacaagcgat ggtattctta ctgctgaaat gtttcaaaag atcgtaaatg gagaaggtgg
    18001 aacatatatt ttgccgattg ctactcctga acaattagga ggtataaaag ttggggaact
    18061 cttagaagtt actgaagaag gtgttttatc tgcaactaag caaacagact tcaatttctc
    18121 tgaagaatta aagggaaaat tagaatctct aaaaatatta aaagcaggtg caaatatatc
    18181 aattgcagaa gatggaacaa ttagttctac aggtggtagt gggactggtg gtgttaatca
    18241 gtcatatgta gatcaaaaat tccaagaagc tgtaaatcaa gcagaaagtt acacaaacga
    18301 aagaattcca aattttactt ttgaaaaaat cggggaggta tagaaaatga cagatatcgt
    18361 aaaaataaaa caaagcggtg tacaggttta tccacaaact cattggaatg ctatagaagg
    18421 caaaccaaca acggtgaagg gagataaagg cgatcctggc caagcagcaa caattaccat
    18481 aggaaccgtt tctagtggtt caaccgcttc tgttacgaat gtaggaacat catctgcagc
    18541 tagatttaat tttgttttac caaagggaga taaaggtgat cctggaataa atgccacaac
    18601 tacagctgta gctacaacta cagcaaatgg gttgatgtcg tccacagata aaactaagtt
    18661 ggatggaatt gcagctggag cacagaaaaa tccaggtaat gctacaacta cgacagcagg
    18721 tttaatgtca gcaaccgata aagtgaaact cgatggatta accaatatta catttgagaa
    18781 agtggggacg gtttaatggc tgatattgtg caattaaaag aagacggagt tgctaagtat
    18841 ttgaaaactc atgccaaagg aattgatggt gtggagggtg ttttagtaaa agccacaggt
    18901 aatgaaacgg tcttaggaac gaaaaatttt aaagacggat tgcaattcaa tggattgcct
    18961 gtgcaagcag gtatgatcga gcgagctatc acactagctg atcgaagtga tacaacaaac
    19021 gtaactgatg tgaatggaaa aataatcaga atcggaaaca tcgtatttct tacttttaat
    19081 tttaaatgtg gcacatggcc cgaaggatca gaaactcgtt ggattttaaa aatcccggac
    19141 ggtttcaaac gtgatcaagg ctatccagcg caaaccgcgc tttcactagt tagaaatgca
    19201 agtcagccag cggatgcacg tgcattcatt gatcaaagta gcatcataca agccaaatca
    19261 ggttcaggta gttcatatat ttcaggtatg tggatcactc aagatccttg gccggcttaa
    19321 cagattacta attggaggaa gagtaaaatg aaaacaattt ataaagtatt gtatccaatg
    19381 ggatatgaag aacatcaagt agaagataac tttccaacat ctttaccatt tgtcgaagtt
    19441 ccacctattt tgtttgaaaa gaaggaagat gaaacggatg aaggtttcgg aagaagacaa
    19501 cagtcgcaat tctttaactt cactgaaaat aagtgggaag aagcagttac acaagattat
    19561 tcaaaaaaat tagaactact agaaaattta tctataggtt tacaggtaga taatgctgca
    19621 ttgaaaaaat caaatgaaga gttaacggaa aaagcagatt cgatggcgca gttgaacgcc
    19681 aaactgatgc ttaatgattt aacaattaat aaaaaaattg aagcaataga aaaacaaatt
    19741 ggaggtgctt cataatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt
    19801 ttactgacga tcaagttcga caattcgttc cactatgcat tacagacgaa
//
LOCUS       DACEMK010000050        19446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEMK010000050 DACEMK010000000
VERSION     DACEMK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.051"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..3654
                     /locus_tag="IW715_001599"
     CDS             127..3654
                     /locus_tag="IW715_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1022207.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IW715_001600"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IW715_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1022208.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IW715_001601"
     CDS             7442..7783
                     /locus_tag="IW715_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022209.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IW715_001602"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IW715_001602"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1022210.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IW715_001603"
     CDS             9140..11560
                     /locus_tag="IW715_001603"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1022211.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW715_001604"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IW715_001604"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1022212.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IW715_001605"
     CDS             12994..13731
                     /locus_tag="IW715_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022213.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IW715_001606"
     CDS             13745..14560
                     /locus_tag="IW715_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1022214.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IW715_001607"
     CDS             14573..15220
                     /locus_tag="IW715_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1022215.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IW715_001608"
     CDS             15236..15892
                     /locus_tag="IW715_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1022216.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IW715_001609"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IW715_001609"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1022217.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IW715_001610"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IW715_001610"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1022218.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IW715_001611"
     CDS             18234..18752
                     /locus_tag="IW715_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1022219.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IW715_001612"
     CDS             18859..19359
                     /locus_tag="IW715_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1022220.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6539 a         3340 c         4175 g         5392 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggaca
//
LOCUS       DACEMK010000051        18737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEMK010000051 DACEMK010000000
VERSION     DACEMK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.052"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            119..766
                     /locus_tag="IW715_001613"
     CDS             119..766
                     /locus_tag="IW715_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1022221.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1159..3804
                     /locus_tag="IW715_001614"
     CDS             1159..3804
                     /locus_tag="IW715_001614"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1022222.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4114..5427
                     /locus_tag="IW715_001615"
     CDS             4114..5427
                     /locus_tag="IW715_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1022223.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5417..6070
                     /locus_tag="IW715_001616"
     CDS             5417..6070
                     /locus_tag="IW715_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1022224.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6125..6796
                     /gene="radC"
                     /locus_tag="IW715_001617"
     CDS             6125..6796
                     /gene="radC"
                     /locus_tag="IW715_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1022225.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6691..8043)
                     /locus_tag="IW715_001618"
     CDS             complement(6691..8043)
                     /locus_tag="IW715_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1022226.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8109..9398)
                     /locus_tag="IW715_001619"
     CDS             complement(8109..9398)
                     /locus_tag="IW715_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1022227.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9485..10189)
                     /locus_tag="IW715_001620"
     CDS             complement(9485..10189)
                     /locus_tag="IW715_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1022228.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10389..10655
                     /locus_tag="IW715_001621"
     CDS             10389..10655
                     /locus_tag="IW715_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022229.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(10823..11053)
                     /locus_tag="IW715_001622"
     CDS             complement(10823..11053)
                     /locus_tag="IW715_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1022230.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11125..11280
                     /locus_tag="IW715_001623"
     CDS             11125..11280
                     /locus_tag="IW715_001623"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022231.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(11589..11813)
                     /locus_tag="IW715_001624"
     CDS             complement(11589..11813)
                     /locus_tag="IW715_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1022232.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12080..12415
                     /locus_tag="IW715_001625"
     CDS             12080..12415
                     /locus_tag="IW715_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022233.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12648..13589
                     /locus_tag="IW715_001626"
     CDS             12648..13589
                     /locus_tag="IW715_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1022234.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13591..14481
                     /locus_tag="IW715_001627"
     CDS             13591..14481
                     /locus_tag="IW715_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1022235.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14524..15324
                     /locus_tag="IW715_001628"
     CDS             14524..15324
                     /locus_tag="IW715_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1022236.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15339..16190
                     /locus_tag="IW715_001629"
     CDS             15339..16190
                     /locus_tag="IW715_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1022237.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            16187..16546
                     /locus_tag="IW715_001630"
     CDS             16187..16546
                     /locus_tag="IW715_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1022238.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            16561..16722
                     /locus_tag="IW715_001631"
     CDS             16561..16722
                     /locus_tag="IW715_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1022239.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            16719..17045
                     /locus_tag="IW715_001632"
     CDS             16719..17045
                     /locus_tag="IW715_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022240.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            17042..17857
                     /locus_tag="IW715_001633"
     CDS             17042..17857
                     /locus_tag="IW715_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1022241.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            17996..18307
                     /locus_tag="IW715_001634"
     CDS             17996..18307
                     /locus_tag="IW715_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022242.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            18304..18534
                     /locus_tag="IW715_001635"
     CDS             18304..18534
                     /locus_tag="IW715_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022243.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            18541..>18737
                     /locus_tag="IW715_001636"
     CDS             18541..>18737
                     /locus_tag="IW715_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1022244.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
BASE COUNT         6317 a         3101 c         3804 g         5515 t
ORIGIN      
        1 aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt gtaaccttgc
       61 tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga gcgtcgttat
      121 gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt tatatttacg
      181 ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg agttcagcga
      241 aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag gcgagctagg
      301 cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata atatccttaa
      361 tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca aagcattgaa
      421 acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc
      481 agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc gtttcgcgga
      541 gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta aatattcaca
      601 atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga
      661 cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa ccgagttgca
      721 gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa atttgagtag
      781 gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag tactaaatga
      841 agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata
      901 acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta tctgctgaaa
      961 ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat ggagtagaga
     1021 tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta ttgcaattta
     1081 ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac gttttttagt
     1141 ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata taatccgcaa
     1201 gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa accgagcgga
     1261 gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac tggaaaactt
     1321 catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca aaagagaatg
     1381 caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc gacacaagca
     1441 aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg tcgtgaaaag
     1501 ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg cgaacagtgg
     1561 gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga tgatggatta
     1621 tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt
     1681 gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat cgaagttatt
     1741 cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga tggatcaggc
     1801 gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc gattgctgtt
     1861 catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct acctttagtc
     1921 aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg gacaggtgta
     1981 gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg tcatgatctt
     2041 ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg aaaatatgct
     2101 ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga
     2161 ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt
     2221 gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc
     2281 attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt caatcaaacg
     2341 tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt atggtgggga
     2401 catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca
     2461 ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg gtttagttca
     2521 gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga ttataagcga
     2581 tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg ggtaagcaga
     2641 atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt cttgattcat
     2701 ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa tggaatcgat
     2761 ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt atcgaatggt
     2821 tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt
     2881 atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg aatgaccgta
     2941 gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct tactcgtttg
     3001 aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt
     3061 cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga aatgagtaaa
     3121 gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat cttagtccat
     3181 acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac agaagaaata
     3241 tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc tgttgtacgt
     3301 ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga attgattcga
     3361 gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat tacgttgcta
     3421 attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag ctatatcgaa
     3481 cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc agatctagcg
     3541 atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt
     3601 gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt
     3661 gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga agtagtagag
     3721 gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa agaacgaatc
     3781 gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca gctagctatt
     3841 actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca ctcttagctt
     3901 cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc tactcttatt
     3961 atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag ttgataaaat
     4021 cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat gtgttctatt
     4081 tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc aatagaatgg
     4141 atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga agctttatta
     4201 gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg aacaaacgga
     4261 aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct gacagtaggt
     4321 acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc
     4381 attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat caaacaactt
     4441 gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc aatggcattt
     4501 gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt aggcggctta
     4561 ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat tggacttgat
     4621 cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc
     4681 aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac tgtcatccaa
     4741 caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta taaaataaac
     4801 tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga aataggaaag
     4861 atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc aggagtagcc
     4921 atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt
     4981 ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag taacgagcca
     5041 ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt tgagaatttg
     5101 aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg aactaaaaat
     5161 gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta tttgacgacc
     5221 tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag tgagagagta
     5281 tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat gacaaatgaa
     5341 gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca tttattaata
     5401 caattaggag gagatcatga agtataatgc agtgattttt gacatggatg gtttgctgtt
     5461 cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga tgggttttcc
     5521 ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag tatgggcgaa
     5581 ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca tagatgattc
     5641 ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc ccggtgtgtt
     5701 ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt ccagtaacca
     5761 acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat ttgagacgat
     5821 tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct tgttagctaa
     5881 tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac aaaatggtat
     5941 tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc ttccaccatc
     6001 ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa tccctgcatt
     6061 gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga aaggagatgt
     6121 ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa gggaacgtat
     6181 ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct tactgcgtac
     6241 cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg
     6301 actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag ggataggacg
     6361 cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc agacggatca
     6421 tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat tgattctgga
     6481 aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga agaatcaagt
     6541 gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc atcctagaga
     6601 aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc ataatcatcc
     6661 ttccggaata tataagttag tacacataaa ttatataagg aaatttactg ttatattgcc
     6721 atctggaaat actattatgt tatcgataat tgaacgccat aatgcatttt tttcttcttc
     6781 cgaagattct ttataaattt catcgaattc ttttgataga ataaattcga cattttttat
     6841 atctatttta gctggctcta cttcaatttc atttaattct ttcattaaag cttctcgatc
     6901 ttttttgtat tcctctaagt ctataacttc attcaagtag gctttcttca atctatctaa
     6961 tttattctca atatttttct tactattttt ctttttaata atctttggtt gtttttttga
     7021 atagtttatt tgtatttgtt caaatttttg tttagtagct tcaaaaatat aacgttctaa
     7081 atttttttcg taaaaagcgc gcttatttgt gcaacgtaat ttattatttc ttcgttttac
     7141 acatctataa caagtccttt cgtatataga ttctgtaccg tcttttttct tatatcttgc
     7201 tttaatagtt tgaccagcta ctgatccctg acagtcagaa cattttaata aacctttaaa
     7261 aatgtaatca cgttttgcat ttgtttttaa atttttaggt aaggcttttt gaacagcata
     7321 aaaaagtttt ttatcaataa taggaggaca gtaattttta ttatttctta attcaccaat
     7381 gtattttttg ttcgtcaaca tatttttaac actttgataa tctctaacga ttccatactg
     7441 agagcctaaa taataaacag tttttcttaa actaccacta gaaagaaagt agttgaatat
     7501 atctatcacg ataggagcat catcatttat tactaatttc ttattttcta tagagtagcc
     7561 taacggagtt ttcccgctta ctacttcacc ttgtgcaatt ttatttgcca taacagcttt
     7621 gatgcgttct gaatcaattt gggcttccaa ttcagcaaaa ctcattactt gtgcaatgaa
     7681 agttctgcca tacgctgtag ttgtatcata atattgttga gaaacagcat tccaagaaac
     7741 attgtgtttt tctaaaattt cttgagtgtt taaataatgt cgtagatttc taaaccatct
     7801 atctaatttt gtaaataaaa ttagatctat tttattttct tttacatctt gtaatagttt
     7861 ttgaaattca tcacgattta ttttttgacc agaaatacca ccgtctacat attcattaac
     7921 tactttcaaa tccttgtgtt gttcgatata ttcatagagg gtttcttttt gttctctaag
     7981 actatctcca tgtttggctt gttgttcagt tgatactctc atatataatg ctactcgttt
     8041 catgttatct cctttcgaat gtatgttctt atcgttgtaa aagaaaagcc cgaaggctaa
     8101 tctttttact acacatctgt taattctaat gatcttctgt attgttcagc ttgagctagt
     8161 ttagtaaaag atacagttcc gttataattt tctaaaacaa ctttttcaat ttcatcaaga
     8221 gatactttaa agaattcttt tcgttgatta actttattga cttcgttttt tctaaaatgc
     8281 gaatgtagtt gattttctaa tgttggtgca tcttctgaaa atatcattgc atgaacatca
     8341 aattcaaaag gtacagaagc gctactaagt tccttaattc tatccatagg ttctaaccgt
     8401 ctagtcattc ctattttata gacatcttca ccgaaagatc cgatattact gatgacataa
     8461 acatagccag ctctagtatt aagttctctt tgcaatacat cttctttgac agtttctagt
     8521 tctcttaatt tctcttccag ttctttaatt ttatcagcat agagttcttt ttcaacatca
     8581 gcactagctt tttgcatgta tgccattaac ttagttatct ctgagttaaa ctgtcgttct
     8641 tctttatcaa tgcgtttctt ttcacgttct atttcacgtc ttaccttttc ttcttcaatc
     8701 atttgttctt ttattgcttt ttgctgttct ttttcatcgt taactttaac tatgtatgaa
     8761 tagttgagtt ctaattcttg aagttttaat tcaagtaatt cttttttgaa ttctgcttca
     8821 tcgtttttaa aaagcttgtt tattttatca aaagttctta taattttttc tctagttgaa
     8881 tctacgttag aaatagtaag tttattgaga agtccagtta tttctgattc taatggtata
     8941 agcattttct ttttaatatt attagcttct ctattattca aataagaagg aatattaatt
     9001 gcaccgtcta aaataaaatc cttttcttta atctttaata aagaaagttt gttttttatt
     9061 tcggatgaag aagtttcatc tatattcgga ataattactg atttatagat agtgaccaga
     9121 gatgtgtcat atttttgatt aatttcatcg tctaaccgct ggctttcagc attaagaata
     9181 tcaattttgt tttctagcgt tgacaagttt ttattcaatt tacttatgtt tttctctttt
     9241 tcagatataa acgtttccct agcatttaat tcttgagaat attcctttac cttactctct
     9301 aaggcagaag ttttatcttt taatttttta gcttcagata atttgctact acccataatt
     9361 attaaaacta ttccaactaa aaacaatagt actcccattc aaaacgcctc gcttctttta
     9421 taaaataatt gtcaaaatca tataaaccct taccgaagtt gacggctccg gcatgggaat
     9481 ttttttaaaa tacaactttt ccgataattc taatctgttc tggcgataca attctatctt
     9541 cgtataagtc gtttatagaa cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct
     9601 ctttacatgt aactccgtca ccacctaatt ccacaatagc aatttcccca tcttctacgt
     9661 ccggttgttc tttaacgaat acgaattgtc cattcctgat aacaggttcc attgaatcgc
     9721 ctataatagg tactgcgaag tcagcagcac ttggaactct ctcaaattct tcctcttcga
     9781 caaccgtatc accgtaagta agttctgttg gattggcagc cgaagccgaa ttgtgaacag
     9841 ttgggatttt ttctttttta ggaaaatcta ggacattttc ttgtttttgt tcttctaatc
     9901 gattacttgc aaattcatag gtagcaactt tattttcgtc gttaagctga ttatatatag
     9961 gaaggatatt atatatctga ttagatatag ataaatcaat attcatcaaa tcatctatat
    10021 caacattgaa tatcttagct atctcattta aggtttttaa ttttggtgta tattttcctt
    10081 tttcccattc gcttattgag gatgaacttt ttcttcctag tttttcggct agttcttgtt
    10141 gatcaatatt atacttttct ctaagaaact ttagattttc agcgaacata gtaacacctc
    10201 cttgagttaa ttgtaacacc gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt
    10261 agtgttttta aagaagtatt tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg
    10321 cacttgactt cggaaaaacc gaagtatata atttagtcag aaagttaaag agaggaggcg
    10381 aataaaatat ggataccgcg aagaaagaaa aatatacatt gagacaatta agagctttaa
    10441 agggttattc aaaagaagaa ctgtcaagga aaagcaaagt gacatcaaga acaatattta
    10501 tttacgaaaa cgatgtagat aaaatgagga acggaaagta cgccactcta gaaaaaattg
    10561 ctaaggcatt gggtgttaga gtttccgata tttttttaga ccctgattcg gaaaaaccga
    10621 aatcaataac aggtgaaaca aaacaagtca gctaggaggt gtgaatagtg gaagagaaga
    10681 atataaaagt accgactctt gaagaatata caaaaattgt tgtcgaaaca gacgagcaga
    10741 acccgaaaac aatcgcagtt attacggcga atgatattga atcatgcgaa ggttttcgag
    10801 ttcgaatgac ccctagatat gattaatgtt ctttatcacg tggaggtata ggatcgtttc
    10861 caaaactatc ttttgattgt atttgtccat tctttttttg gacaataaac tctgattgct
    10921 gttttttaga aatcttttta ccatactcaa cagcatctga tttattatca aatagttttg
    10981 tatcttcgga attaccttca ccacgtacag cccatttgcc atttctagga gatacccatt
    11041 gatttttacc cataacaatt cacctcgctt tcaagttaat tctattaaga ggtgaaaaga
    11101 aaacaaactg atggaggtga gattgtgagt aacaagccaa aaatcacttt gaatggcaca
    11161 cctaatcttc aaagattagc agatgaaatg gctagaatac gttctagaca ttggggagtt
    11221 actgtggtgg caaccataat tgaacaaaaa gaaaagccca cacagccggc aagcaagtag
    11281 tgggcaccat agaacaataa tattatatca cacactcgcc acttccgaaa agaggagttg
    11341 ctgctctcct ttcaagtttg ccaaagtcca cgtttcttca ttttggcaac tcctcttctt
    11401 ggaggtggtg acggcgagag acgaaggagg gatgacatga aggaagaatc aaaaataggg
    11461 attttgaatg agaacaatta cacaagatta attattgaaa cagaagaagg ggaaaaagta
    11521 gcagaagtta cattgaccga tgcaactcct gctgacggtt atcgtattcg attaactcct
    11581 aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt gactatcacg gctttgaatt
    11641 tttccatctc tgccatgaat aaatagctca gatccttgat ttttagaaat ctctctagcg
    11701 atttttactg cttctgattt tgtattagta tgtgcagtcg ctttagaatt tccagcacct
    11761 ttgacattcc aaccaccatt ggaattaggt acaacatgtt gattttttcc catagttttc
    11821 acctccattt ctatagaaat attttatcaa atagaaatgg agaaattcaa taaacgaaag
    11881 gagggatcaa atgacattac aagagcatca acacttgatg cgaaaactaa atcaggaata
    11941 ccacgaggaa atgaaaccta gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg
    12001 gttagaaata aaaaagtgac tcagccgtcc aaagcaatga gtcacaaacc aaaaattata
    12061 cctaaggaga ttttatcaca tgaataagaa aattgaaaat cttatcgaag aattgaaacg
    12121 tgaatgccaa aagcaaggtg tttctattat ttgtacagcc caaaaagaag gggaacttaa
    12181 aagtctcgtt tatggtgaga caacagaaat tttactttgt cttgctatgc aagaagaaca
    12241 tttagatgaa aatctcccat tgtcagcaca tattatgcgt agaattgctg tggatgctta
    12301 cgaacaagcg aaaaatgaag aagaaaatca accttcaaat catacttttg ttattaataa
    12361 caaggaagat ttagctgatg tgatgactcg tattctcaag ggggaatttc aatgatgaat
    12421 gaaaaaacaa ttgatctact cattatttta tttttcacgt tagtgattgt agcgaaaagt
    12481 tatgtaatct ttggcttgtt attcgcaatt accatgcttg tgctttgcgt gttagcaagc
    12541 agaaaagagg ttaaacatga acgagttagc aaatcttgac aactatttaa ctgatcctga
    12601 ttatgctaaa ccaccatatg aagcgccaat tgatgaggag gatgaagatg agtaaatcta
    12661 ccttagaaat gagccatcaa gaatggctcg aagaccgcaa aagaggtatt ggtggctcag
    12721 atgttgcaac cgtacttgga ttaaacaaat acaaatctcc ttatcaattg tggcttgaga
    12781 aaacgggtca aattgaattg aaagatttag aaagcgaacc agcttattgg ggtaatgttt
    12841 tagaagaagt ggttgctaaa gagtttcagg aacgtacagg caaaaaagta cgcagaagaa
    12901 accaagtatt tgaacatcca ttacatccat ttttaagagc aaatattgat cgggaagtag
    12961 tgggagaaaa tgccattctg gaatgtaaaa cagccaatca atttctcgga aaagagtggg
    13021 aaggtgaaga ggtaccgctt agctatctct gtcaagttca gcattacatg aacgttttaa
    13081 acaaagacta ttgttatatc gctgttttga tcggtggaca aaaattcatt tggaagcgga
    13141 ttgaacgaga tcaagagctg atcgatacaa tcactgaaca attagtagag ttttgggaaa
    13201 cgaacgttct tggaggtatc gagcctgtaa ttgacggaag tggagcgact gctgacttct
    13261 taaaagaaaa atatgcagat gtagaagaaa atcaaacagc tctaccatca cgttttgatg
    13321 aacttatcga gcaaaaaaga gaactcaagc ggacgaaaaa agaaattgaa tcatctattc
    13381 gtcaagtaga caacgagatc atcagtgagc taggtaaacg tgaagccagt atcggtatca
    13441 cacaaagaaa cattatcagc tggaaacttg ttcgtacaag acggatgaac acgaagaaac
    13501 tagcagagaa atatccagat gtcgcaaatg atgaagagat ttataacgtt actgaatcaa
    13561 gaaggctaac cgaaaaggag atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa
    13621 ttggcagaaa agccacagaa acaagttgca ccaggacagt tagggcttaa agctctaatg
    13681 aatacaccaa caatgagaaa gaaatttgaa gaagtacttc atgacaatgc taatgctttt
    13741 atgtcgaatg ttatgactct tgtatctaat gacagttatc ttgcagatag tgaaccgatg
    13801 tctatcatga gtggtgcgtt aactgctgca acattaaatc ttgggctaga taagaattta
    13861 ggttatgcat atttagttcc attcaatagt aaaaacaagc aaacaggaaa atgggaaaag
    13921 aaagctcaat tcatgcttgg ctataaagga tatatccaat tagcccaacg atcaggtaaa
    13981 tacaaagcat taaatgtgat cgaagtttac gaaggagaac taaaaagctg gaaccgactg
    14041 acagaagagt ttgagtttga tccaaatggt agaacgtctg atgaagtcat tggatatgtt
    14101 ggctatttcg agttactgaa tggattcaaa aaaactgtct attggaccaa acaagaaatc
    14161 gaagctcatc ggattgctaa caataaagat cgcgataaga caaagttaag tggtgtgtgg
    14221 gcatctgatt acaatgcaat ggcacgaaaa actgttttga gaaatcttct ttctaaatgg
    14281 ggaatcttgt ccatcgaaat gcaagaagcc accacatcgg atgagagagt ccaaagggtt
    14341 caagaagacg gcagcattat tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa
    14401 gaagcagagg ttatatctga agaaaatgaa gatgtacaaa ctggattatt tgatgcatct
    14461 aatccgccgt taaacaaata atgagggagc tttctccctt gcttttctag aggagaaata
    14521 cgaatgaaca caggatatgt caaattacat cgaaaagtga tgaattcatt cgtttggacc
    14581 aatccttata tgtataaatt atggagcttg tgtttgatga aagcgagtca tgagaatcgc
    14641 aagatacttt ttaatggaaa agagatacaa gtgaacagcg gagaattcgt cacagggcgc
    14701 gatgctatca catctgagat gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt
    14761 tctgtatgga gatggctaaa acaatttgaa aaaaacggaa tgttgaacat caaatcaact
    14821 acgaaataca gcgttatatc aataaaaaac tggtctttgt accaaagcag tgaacaacaa
    14881 atgaacatca aacgcacaac aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat
    14941 tataagaatg aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac
    15001 gacaccgact cagtttacta cattctcgcg gaggagttat tcaaacagat ttgtcagaat
    15061 caggaaatca aaaagccgaa tctgcaaagt tgggcagata acattcggaa aatgatcgag
    15121 atcgacaagc ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca gcacaatgtg
    15181 ttttgggcat cgaatatttt atctgctaaa aaattgcgag aaaaatacga cacaatggca
    15241 gcgcaagcaa atcgtgatta taaaacaaaa caaactaaaa cgcttgaata cgagaaattt
    15301 agcacagatg agttgcctat ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca
    15361 tggaaaagct gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc
    15421 ctttgtatgg atggcgtacg aagaaccctg atggctcaga acgttgtaag ccaacctgta
    15481 tgcaatgtgg gtataaagca ttgcgagttc aggaagactt acaaacagaa cgaatttaca
    15541 acgagagcct gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc
    15601 aagcgttgtt tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa acaagacaag
    15661 cggttgaagt aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca agtcaccttg
    15721 ttttaactgg aaaacaagga acaggtaaaa gtcacctagc gatggcagcg gcttgggaag
    15781 tcttgaagcg atcaaactac gataaaaaaa tcttatttat cgggttacag gaaatgctgg
    15841 atcaaatcaa attttcgtac aacaatcctg aactcagaaa aacgattgag ggatcgttga
    15901 ttgcagatat caaaacagcg gatttagtca tcattgatga tatcggttca gaactaggaa
    15961 aagatgcatc agatagtcga gcgtttggca taaacacgct aaattcgttc ttggatgcac
    16021 gacagaacct agcaacgatt atcacaacga acttgcttgg tgaagaactg aaaaaagctt
    16081 acggtacgag aacaatatca agaatgtttg tcaactctga tggatttacg atggtatttt
    16141 ctcaaacagc agacaagcgc ataaaaccag tgaaaggtag tatcgcatga ataaataccg
    16201 taataaaaaa actgttcatc gaggtatcaa gtttgattct atcgcggaag cagagtatta
    16261 cgatctagcc ttgtggcaag ctgaagcgaa tggttggaaa gtgaagctcc aggaaaggtt
    16321 tgagctgatg ccgaagtttg aactagaggg caagaagtat cgcaagatcg agtatattcc
    16381 cgacttcaca ttttacaaaa gcggcaagct ggtcaaagtc gtagatgtta aggggatgca
    16441 gacaaaagac tttaagatca aggcaaagtt gttctgtcat caatatcaag tgccgttgat
    16501 attagccaaa aaatatcgga atacgttcaa ggaagagcgt ttttaacgag gtggtccatc
    16561 atgacaacag aagaagtgat tcaaatgcga attcgaagcc ttcggcgtga gattgacgat
    16621 ctggaacgaa caaaggcagt gatggtcaat gaaacggcta gaaaggcaat cgatttgcac
    16681 atagagaatt taagaaggga aatccgtaga ttggaggaat gagcgtggat aaggaagcgg
    16741 cttggcgaaa attaatgttg ctgattcaag atgagaactg gcaagaagat gaagcagtgg
    16801 ttgctgaagt tcagcgtcta ggaaagattg ctaacggacg tatacgaaaa aagccagaca
    16861 aaagaaaaca gcgcaaaggg aaaatcgtcg ttgttttaca cgaaggcaaa attttgatgc
    16921 aaggaacagc tagtgagctg tctgcagaaa ctggatatac gcgtgggact attcgaacgt
    16981 acgcttggcg aaatcacacc gatcgaaaag ggcacgaata taagtatttg gaggaagaaa
    17041 aatgaacgaa aacaaattaa tcaaattggg tgtagcagga gcagtaatcg taggtattgg
    17101 agttatcgga ggatttaagt tcttcgaaaa aatcgataat ggatatgtgg gtgtgcgcta
    17161 ttcaatgaac ggcggtatca aagatgaagc actgacgcaa ggtgtgaaat ttgtagggat
    17221 tgacaaagtg atccaatatc caattcgctt gcaaactatc caatcaaaaa atatttcagt
    17281 atctacaagc gacggcaaaa agacaacgat tgatatcaaa tatgactaca aagttgattc
    17341 aactaaagca gcaaaaatgt acaaggaatt tgggaatatc acttcggaag atatcgaaag
    17401 tggatggtta aaatctaagc ttcaaaaggt cgctcgtgaa gtttatgcga aatatagtct
    17461 gcttgatgtc ctttcaggag attcctctaa agttgaagct gaggtattaa cgaactttgc
    17521 taaatcagtt gaatctaaag ggtttgaagt cgaagacgta acacttggtg ttccagatgt
    17581 cgataaagaa acacaaaaat caatcgatgc gatcattcga gctggtcaag aaaatgaaaa
    17641 agcgaagcta gacgcagaaa ctgcaaaaac tcaagctgat agtgaagctt acaagaaaac
    17701 aaaagctgca gaagcagagg cagaatctaa tcgcaaagtc gccgaatcag taacagacaa
    17761 tttgattcgt tatgaagaag ctcaagctcg caaaaagcat ggatgggtaa cagtaaatgg
    17821 agcagatact gtagttacgg atgaagcagg caaataatat gggattcttt atggctaaaa
    17881 ttctcttgtt cttaggttta gtaggagcag catatctcgt gtatgcgctc ctttcccaaa
    17941 ctgatgacaa agaagatgac aacaacgatg aaatgaaatt ttaggaggag aaataatgga
    18001 cgaactaatc acaaaagtag agcagtgggc taaagacaag ggactggatc aagcagatcc
    18061 aaaagcacag tttttgaaag tagctgagga attcggggaa atagcttcgg cgatggcaag
    18121 aagtaatgat gaactattta aagatagcgt aggagacgtt atcgtcacgc tgattatcct
    18181 ttccatgcaa aaagggacaa acgtacaaga gtgtttagaa atggcataca acgaaatcaa
    18241 aggacgcaca gggaaaatgg tagatggtgt attcgtgaag tcgagtgatt tggaggatgt
    18301 gaaatgaaat acgaaatacc actaagtgaa gcgggcattc aagcaattat caatggtcgg
    18361 gaggttaaca tagaacttcc tgatggtact gaattagtca tcagacaaag ttatttgaaa
    18421 gatatggcag ctccagtatt aattgatcgt tttaacgtga ctgattctgt ggtagagaac
    18481 cacttaaaag aatttcgatc aagtatagac aacactttca gattagggag ttgattgaca
    18541 atgaacacca gacaccgcag agtagcaaaa ctaagaaaac aggaactaaa tgtactaaag
    18601 acaaagtttg aaaaagaata tggaatttca gcagaagaaa catataaagt ggcaagtcag
    18661 tttgttgctg atgcaagtga tgctattcgt aagtttggga tttcgatatt aaatgatgat
    18721 cgtaaatggg aggaaat
//
LOCUS       DACEMK010000052        18339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEMK010000052 DACEMK010000000
VERSION     DACEMK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.053"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(131..697)
                     /locus_tag="IW715_001637"
     CDS             complement(131..697)
                     /locus_tag="IW715_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1022245.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(697..1653)
                     /locus_tag="IW715_001638"
     CDS             complement(697..1653)
                     /locus_tag="IW715_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1022246.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1653..2309)
                     /locus_tag="IW715_001639"
     CDS             complement(1653..2309)
                     /locus_tag="IW715_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1022247.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2679..4697
                     /locus_tag="IW715_001640"
     CDS             2679..4697
                     /locus_tag="IW715_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1022248.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5124..7538
                     /locus_tag="IW715_001641"
     CDS             5124..7538
                     /locus_tag="IW715_001641"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1022249.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7886..9439
                     /locus_tag="IW715_001642"
     CDS             7886..9439
                     /locus_tag="IW715_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1022250.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9820..11604)
                     /locus_tag="IW715_001643"
     CDS             complement(9820..11604)
                     /locus_tag="IW715_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022251.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11619..13340)
                     /locus_tag="IW715_001644"
     CDS             complement(11619..13340)
                     /locus_tag="IW715_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022252.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13440..14201
                     /locus_tag="IW715_001645"
     CDS             13440..14201
                     /locus_tag="IW715_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1022253.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15168..15436
                     /locus_tag="IW715_001646"
                     /pseudo
     CDS             15168..15436
                     /locus_tag="IW715_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16108..>18339
                     /locus_tag="IW715_001647"
     CDS             16108..>18339
                     /locus_tag="IW715_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022254.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6016 a         3272 c         3712 g         5339 t
ORIGIN      
        1 ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa gaggttatga
       61 aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat ttatgttttg
      121 cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg ttcttttggt
      181 tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa caaaaagaat
      241 caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca aggatcaatc
      301 gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta gtctctggca
      361 tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc cctgctttga
      421 gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga aacaatgaga
      481 cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt gcttgttgct
      541 ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga aaaaccgtat
      601 cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag atttcttgca
      661 gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct ttttccgtac
      721 atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg cattcaagac
      781 ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg catcccctgt
      841 caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa tgacattgac
      901 atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca acattctcgt
      961 tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat ctaaaatggt
     1021 gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt tgatcaaatg
     1081 cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat aagtttgata
     1141 atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca atccaagttc
     1201 tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt catctggcag
     1261 acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga ctacttgctc
     1321 aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt ggaaataaac
     1381 gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt tctggaattg
     1441 gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata cactgcaaaa
     1501 agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc ctccatcgat
     1561 cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc aagaatgata
     1621 tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct gtaagagtta
     1681 tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca agaaagactg
     1741 agtaagtagc cgccgatgat atcagtagga aaatgaacgc ccagataaat gcggcttatc
     1801 ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa ggttcttttt
     1861 tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact gcctgctgca
     1921 tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga aggacgctct
     1981 cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat aaatccagaa
     2041 acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc cgcacaagca
     2101 aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg gtagctgttc
     2161 agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc agaaagccac
     2221 gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa gaagctacca
     2281 gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa tgacttatta
     2341 tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta tgattcctct
     2401 ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat tttttggtaa
     2461 tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt aaacaagaac
     2521 gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa attacaatat
     2581 gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga gaagttataa
     2641 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
     2701 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
     2761 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
     2821 aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag
     2881 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
     2941 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
     3001 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
     3061 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
     3121 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
     3181 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
     3241 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
     3301 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
     3361 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
     3421 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
     3481 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
     3541 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
     3601 cgaataattc cggtaacaat accaactctt caggaaatgc tggtacatca aacggaggac
     3661 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     3721 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     3781 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     3841 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     3901 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     3961 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     4021 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     4081 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     4141 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     4201 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     4261 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     4321 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     4381 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     4441 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     4501 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     4561 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     4621 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     4681 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     4741 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     4801 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     4861 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     4921 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     4981 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     5041 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     5101 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     5161 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     5221 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     5281 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     5341 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     5401 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     5461 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     5521 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     5581 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     5641 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     5701 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     5761 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     5821 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     5881 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     5941 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     6001 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     6061 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     6121 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     6181 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     6241 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     6301 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     6361 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     6421 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     6481 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     6541 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     6601 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     6661 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     6721 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     6781 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     6841 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     6901 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     6961 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     7021 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     7081 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     7141 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     7201 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     7261 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     7321 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     7381 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     7441 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     7501 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac
     7561 tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt
     7621 tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac
     7681 acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt
     7741 ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc
     7801 aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa
     7861 tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc
     7921 agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct
     7981 tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg
     8041 ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca
     8101 cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat
     8161 ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca
     8221 acagttttta cataataaac caacttctca agatgatgac tatctttatt ttgagaagac
     8281 ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact
     8341 tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca
     8401 tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata
     8461 cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac
     8521 aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca
     8581 catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc
     8641 tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac
     8701 agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat
     8761 tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg
     8821 ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc
     8881 tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat
     8941 tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg
     9001 tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac
     9061 tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca
     9121 aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta
     9181 tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca
     9241 gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac
     9301 gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc
     9361 ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca
     9421 agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca
     9481 agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga
     9541 gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt
     9601 gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca
     9661 agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt
     9721 attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga
     9781 ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact
     9841 attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc
     9901 gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg
     9961 gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa
    10021 tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt
    10081 cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt
    10141 ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg
    10201 tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat
    10261 attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg
    10321 acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt
    10381 attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg
    10441 accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat
    10501 tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc
    10561 taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat
    10621 cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat
    10681 tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc
    10741 acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat
    10801 gaaacgaatg gccggataga tagcacagtt cataaattga gcacgtttaa aagcgttgta
    10861 ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt
    10921 gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata
    10981 ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact
    11041 gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag
    11101 accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac
    11161 ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt
    11221 gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa
    11281 tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct
    11341 taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact
    11401 gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat
    11461 tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa
    11521 tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt
    11581 gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca
    11641 aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt
    11701 tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca
    11761 acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct
    11821 gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc
    11881 aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga accgccttga
    11941 gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc
    12001 ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt
    12061 aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc
    12121 tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg
    12181 tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg
    12241 atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag
    12301 atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt
    12361 ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct
    12421 tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc
    12481 atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt
    12541 aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc
    12601 atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga
    12661 ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag
    12721 gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt
    12781 tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat
    12841 acataaaaaa caacagagat caatgtaacc catgttagta atagagaata acgaaacatc
    12901 atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca
    12961 atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta
    13021 aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt
    13081 tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt
    13141 gttcctagaa acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg
    13201 ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg
    13261 gcgatttcta aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag
    13321 aatgttttta agatagacat attattcctc cttttttcct gatacttttc gattctatac
    13381 cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa
    13441 tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga
    13501 tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc
    13561 gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag
    13621 atataccgat caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga
    13681 cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa
    13741 aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat
    13801 ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg
    13861 aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca
    13921 gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag
    13981 acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga
    14041 tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat
    14101 ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca
    14161 gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata
    14221 actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt
    14281 aatgtggggg attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc
    14341 tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa
    14401 aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat
    14461 gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa
    14521 taggtgcata aaagcattac acttaaaata cattgttgct tacattatta caatatgtta
    14581 ataaggcaaa aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac
    14641 tgtgactttc ctcagctatc ggtgatacta ccgatagcat ttttttgctt ataaaataat
    14701 tataagtatt tagagatttt tcttctaaca ttcttcaaac aagaaaaatc acaaaggtta
    14761 ttgttatttt tatatattct ttttaagaat gattgttttt ttatagaaat tttaaaaaat
    14821 aaaacacgtt attgatggat agttagtttg ttatttggta atattataga agttgactgg
    14881 tagtgattta tttttcgtcg ttttataaaa atgttatttt ttggaaaaat tcacaaatat
    14941 gtaacgaatg tttttctgga atttgctgtg ttaaaaagca gtatgactaa tataaatact
    15001 attgtcattt atgcaatttt ttatggatag taattttaga ggggaatcaa tgaaggaaga
    15061 atagaaggga aaaatagttg caaatacgcc agttagcaaa aatagcaata catcttcaag
    15121 ctttaaaatt cgagagaatc atcttggtag tggaatcaac tacaacaaaa ggattaaacc
    15181 aattaataga tagaattacc aatggaaaaa tagaaaaggt agttattcta tacaaaagtt
    15241 gtttaattcg cttcggttat gaacttagtg aaaccctttg caatatatgg tacagaatat
    15301 agaaattatt gacattacag aaaatgatga agatttaata cagattgtca ctatttgtag
    15361 atacctttta caaggaaacc gagccaataa agctaagaag atgattaagg agttgatgga
    15421 agacgataca agttaagaaa gtgaaactac gcccaaataa agagcaaaaa gagtaattgg
    15481 acgtggaaaa tgcaagaaat gaaccatcga ttcggtggac agtttattag taatgacggt
    15541 ttgagaacat ccatgacgag aatgaaggaa catcctaaat gcgtttagtt gaaccaagtg
    15601 ccaaataata tggccaaata agcgatcagt tatcgatgaa tcccaagtat tctaaactat
    15661 atattcattt tgatgctaag cattgatata tttttttggg attgatgaag gttaaccaat
    15721 acagggactc actgatgaaa ttctagagtc tgaccttggt atcaacgatt tagtgattgt
    15781 ttctaatgga aaagtataaa agtatataag tacattaaca aaataactat tagggttacg
    15841 ctccagcttg tgcaaggcac tggcaggagg aatcgtgaaa tcataattat ggttaagcca
    15901 cacggacaac taaaagtcat acaaagctat cgaatcaatc aacagaagac aatcggcaat
    15961 cattagcttt gtttacagct tacattgcaa cgcttgagtt gaaccgaaaa tacgagggag
    16021 gtaacgcagc cattttgagt tggggaatct ttttagaaaa ttatcgggta ccttgttgtt
    16081 tagtcaaaat tataaggggg agtaatagtg aggaacaaat ttcataaact gttaagtatg
    16141 tttctagtta tgcttattgt gatcaatcaa tttgggaaca taacggttct agctgagtct
    16201 ttagcagaac agccagagcg ttcgctgacg atcgaagttg atcaagagga ggctgctttg
    16261 ggtgagcagt tccagttgcg agtcagagga acaccagacc tgctaaatgc attggtcatt
    16321 gataagagtg aggatttgat tgaaggggaa gtgaagcaac tcaatgatta cgaacgtctg
    16381 attacttatt cttcccagca ttcaggtaaa tacgaattga ccgcaagaag cggtgatatt
    16441 caatcaaata cggtctcaat agaaatcagt gtaccagaag aaagcacaag tgagtctttt
    16501 aatgtaccag aagaaagcac aagtgaatct tttaatatac cagaagaaag cttaagtgaa
    16561 tcttttaatg aagccagatc atctgagcca tctataacgc aggaatctca gattgaaaga
    16621 caagaggagc tcttgaatta tgacatcggc atctctgctg agcgccccac tttgccgagc
    16681 aggcatacac aaacgttaaa gggatacatt caaggaggcg ctggcgatat tcttgaagaa
    16741 tatggggaga tggtcgtacg agttcctaga gcatcgattg gcttaattga gttcattaga
    16801 gtggaggtcg ctgatgattc acctttaaag ccactggcat tttaccaaga aggcacagac
    16861 gttgtttttc catttacctt tgattatgaa ggattaaatg aagccgctca gtcgtataca
    16921 ttcagtatca cgtttgaagt tgctttcttt caatcaggcg gtactgagcc aaataatgac
    16981 tttcaagtga gtcttactta tgctgagcga gcagcaaaca cggaattttc aattacgcct
    17041 attccttcat caagacccat tttttcgaaa ttatactatg gctctagaga aatcgacagc
    17101 caaggaaatc ttgggaatct gctgattgac gggcaagacg tgaccaacag taataaattt
    17161 attttgcagg taaattatga aggatcacga aaagacggtg tagtggttac cgatgtacta
    17221 ccgcaaggaa caactttggc accgaatccg actacatgga gttccactgg aaatagagaa
    17281 cctatttcag gtggggttcg gatcgcagaa gtaacgaatc caggtgaagc aaacgttaga
    17341 actcattatg tttccgagga atttgtggac aaagtatctt ttaataagga aacgaataca
    17401 cttgttgtgg actttggtaa tctagcagag ggtcaatcgt attttattga atatgctctt
    17461 gaagtgaccg atgtcgactt gttcaatgaa ggaagacaag aggatgggtt ttctcggaat
    17521 cgagcgagta tgacctacaa taatcaggta atgagccgag actaccgctt gagaatccct
    17581 gaaagtaatc ttccggcaat ctctttaaga aaaagtgtgg acaaaaatgt tatcaatctc
    17641 aatgaaaatg aactaacgta tactctgcat gtctcgatgt tttctggaac aagaaatacg
    17701 cttgttattg aagatgtttt ggaagaaggc atggaatttg tgtcatttct atcagatatt
    17761 ccctctttca ttgatgccga agtcaataat ggaatagttc gtttcagtgc gaataatacg
    17821 attggtggtc cgacaagttt taccctctct tttaaggtca atgtagaggc gtatgcggtc
    17881 ggcgatcaaa ttttaaactt tgcaacgctt gtgaatagtg acaatcgatt gaatacgagt
    17941 actgtaagaa caaggaaaat cgatgggcgt attaaagtaa ttaaaactga tgagaatggc
    18001 caaaggctgg caggagcaaa atttgaaatt ttatcaagcg attcacaagt gatacaagaa
    18061 ggtataacgg cagaaaacgg agaattttta agtgaaccgc ttcctgtagg tagctatcaa
    18121 gtccgagaaa cagaagcacc ggaagggtat cttttagatg cgacggtaca tgatgttttt
    18181 ataaatgaat actccataga agaagtgacg ttaaccattg aaaacatcct tgatacgggt
    18241 ggtgttgaac taattaaaca tgctgaaggc agtggtgaag tccttcaagg agctgttttt
    18301 gagttacaga accgagaagg agaaacgcta caaaccggt
//
LOCUS       DACEMK010000053        17688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEMK010000053 DACEMK010000000
VERSION     DACEMK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.054"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(136..1716)
                     /locus_tag="IW715_001648"
     CDS             complement(136..1716)
                     /locus_tag="IW715_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1022255.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="IW715_001649"
     CDS             complement(2117..3493)
                     /locus_tag="IW715_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1022256.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="IW715_001650"
     CDS             complement(3621..4739)
                     /locus_tag="IW715_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1022257.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="IW715_001651"
     CDS             4881..5315
                     /locus_tag="IW715_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1022258.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="IW715_001652"
     CDS             complement(5393..5926)
                     /locus_tag="IW715_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1022259.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IW715_001653"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IW715_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1022260.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IW715_001654"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IW715_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1022261.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="IW715_001655"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="IW715_001655"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1022262.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="IW715_001656"
     CDS             complement(9651..10229)
                     /locus_tag="IW715_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1022263.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="IW715_001657"
     CDS             10551..11021
                     /locus_tag="IW715_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1022264.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="IW715_001658"
     CDS             complement(11129..12355)
                     /locus_tag="IW715_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1022265.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13109..13309)
                     /locus_tag="IW715_001659"
     CDS             complement(13109..13309)
                     /locus_tag="IW715_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1022266.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13443..14051)
                     /locus_tag="IW715_001660"
     CDS             complement(13443..14051)
                     /locus_tag="IW715_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1022267.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14136..14606)
                     /locus_tag="IW715_001661"
     CDS             complement(14136..14606)
                     /locus_tag="IW715_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1022268.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14654..15568)
                     /locus_tag="IW715_001662"
     CDS             complement(14654..15568)
                     /locus_tag="IW715_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1022269.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15714..16517
                     /locus_tag="IW715_001663"
     CDS             15714..16517
                     /locus_tag="IW715_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1022270.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16695..17642
                     /locus_tag="IW715_001664"
     CDS             16695..17642
                     /locus_tag="IW715_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1022271.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5364 a         3543 c         2911 g         5870 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagcgcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    13141 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    13201 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    13261 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc
    13321 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    13381 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    13441 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    13501 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    13561 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    13621 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    13681 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    13741 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    13801 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    13861 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    13921 tcaattgctg catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    13981 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    14041 gtgtaagtca taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga
    14101 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    14161 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    14221 gctaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    14281 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    14341 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    14401 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    14461 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    14521 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    14581 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    14641 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    14701 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    14761 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    14821 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacattct
    14881 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac
    14941 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    15001 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    15061 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    15121 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    15181 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    15241 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    15301 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    15361 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    15421 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    15481 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    15541 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    15601 taacattttt ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca
    15661 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    15721 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag acgagaaatg
    15781 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    15841 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    15901 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    15961 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    16021 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    16081 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    16141 aacttcccta caccaatgaa tctttaaaaa acttaacagg taaggagctc cgttcttttt
    16201 taagtaaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    16261 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    16321 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    16381 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacaa
    16441 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    16501 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    16561 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    16621 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    16681 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    16741 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    16801 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    16861 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    16921 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    16981 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    17041 aaaaagcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    17101 gaaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    17161 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    17221 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    17281 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    17341 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    17401 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    17461 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    17521 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    17581 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    17641 aatagctgaa aacaatattt tcaaaagaac aaagagaagg tgacaaaa
//
LOCUS       DACEMK010000054        17516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEMK010000054 DACEMK010000000
VERSION     DACEMK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.055"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            100..2427
                     /locus_tag="IW715_001665"
     CDS             100..2427
                     /locus_tag="IW715_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1022272.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IW715_001666"
     CDS             2553..2882
                     /locus_tag="IW715_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1022273.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IW715_001667"
     CDS             2922..3926
                     /locus_tag="IW715_001667"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1022274.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IW715_001668"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IW715_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="IW715_001669"
     CDS             5558..6730
                     /locus_tag="IW715_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1022275.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="IW715_001670"
     CDS             complement(6892..7347)
                     /locus_tag="IW715_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022276.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="IW715_001671"
     CDS             7936..8358
                     /locus_tag="IW715_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1022277.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="IW715_001672"
     CDS             complement(8524..9717)
                     /locus_tag="IW715_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1022278.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="IW715_001673"
     CDS             9941..10318
                     /locus_tag="IW715_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1022279.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="IW715_001674"
     CDS             10306..10644
                     /locus_tag="IW715_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1022280.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="IW715_001675"
     CDS             10778..11221
                     /locus_tag="IW715_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1022281.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="IW715_001676"
     CDS             11483..12298
                     /locus_tag="IW715_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1022282.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="IW715_001677"
     CDS             12308..12970
                     /locus_tag="IW715_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1022283.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="IW715_001678"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="IW715_001678"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1022284.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="IW715_001679"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="IW715_001679"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1022285.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="IW715_001680"
     CDS             15692..16189
                     /locus_tag="IW715_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1022286.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="IW715_001681"
     CDS             16374..16634
                     /locus_tag="IW715_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022287.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="IW715_001682"
     CDS             complement(16996..17235)
                     /locus_tag="IW715_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022288.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3473 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggata
//
LOCUS       DACEMK010000055        17073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEMK010000055 DACEMK010000000
VERSION     DACEMK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.056"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(9..530)
                     /locus_tag="IW715_001683"
     CDS             complement(9..530)
                     /locus_tag="IW715_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1022289.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IW715_001684"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IW715_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1022290.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IW715_001685"
     CDS             4610..5497
                     /locus_tag="IW715_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1022291.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IW715_001686"
     CDS             5494..6663
                     /locus_tag="IW715_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022292.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IW715_001687"
     CDS             6687..8042
                     /locus_tag="IW715_001687"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1022293.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IW715_001688"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IW715_001688"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1022294.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="IW715_001689"
     CDS             10328..10618
                     /locus_tag="IW715_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022295.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="IW715_001690"
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="IW715_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1022296.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11659..12318
                     /locus_tag="IW715_001691"
     CDS             11659..12318
                     /locus_tag="IW715_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1022297.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..13407
                     /locus_tag="IW715_001692"
     CDS             12475..13407
                     /locus_tag="IW715_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022298.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13561..14850
                     /locus_tag="IW715_001693"
     CDS             13561..14850
                     /locus_tag="IW715_001693"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1022299.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15097..16347
                     /gene="clpX"
                     /locus_tag="IW715_001694"
     CDS             15097..16347
                     /gene="clpX"
                     /locus_tag="IW715_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1022300.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16430..17017
                     /locus_tag="IW715_001695"
     CDS             16430..17017
                     /locus_tag="IW715_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1022301.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5586 a         3060 c         3739 g         4688 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa
    13321 acgatgtatg aaggtacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt
    13381 ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga
    13441 aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta
    13501 aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa
    13561 atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg
    13621 caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac
    13681 gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa
    13741 gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc
    13801 aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa
    13861 ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa
    13921 tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca
    13981 cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct
    14041 gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat
    14101 ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt
    14161 gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca
    14221 gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat
    14281 gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac
    14341 gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa
    14401 gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca
    14461 gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa
    14521 ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt
    14581 tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca
    14641 aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc
    14701 aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc
    14761 gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc
    14821 acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag
    14881 ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc
    14941 cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac
    15001 actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt
    15061 tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat
    15121 gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct
    15181 ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa
    15241 gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt
    15301 gaagtattga ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc
    15361 gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa
    15421 ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca
    15481 caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca
    15541 gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct
    15601 gattacaatg tggaacttgc agaaaaaggg atcatctata ttgatgagat cgataaaatt
    15661 gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa
    15721 gccttgctga aaattctaga aggaactgtt gcaagtgtgc cacctcaggg cggacgtaaa
    15781 catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt
    15841 gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt
    15901 ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa
    15961 gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct
    16021 ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta
    16081 gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa
    16141 gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt
    16201 tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc
    16261 gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat
    16321 aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct
    16381 tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca
    16441 tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc
    16501 agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat
    16561 tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt
    16621 ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt
    16681 atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga
    16741 ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat
    16801 ccagatgtat caatttttga agtattatga tatccctgta attgttgttg ccactaaggc
    16861 agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga
    16921 ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga
    16981 agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag
    17041 tcaaactctt tcataaaagt ttggctggta agt
//
LOCUS       DACEMK010000056        17034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEMK010000056 DACEMK010000000
VERSION     DACEMK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.057"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(271..714)
                     /locus_tag="IW715_001696"
     CDS             complement(271..714)
                     /locus_tag="IW715_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1022302.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(932..2272)
                     /locus_tag="IW715_001697"
     CDS             complement(932..2272)
                     /locus_tag="IW715_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1022303.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2541..3437
                     /locus_tag="IW715_001698"
     CDS             2541..3437
                     /locus_tag="IW715_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1022304.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3587..4528)
                     /locus_tag="IW715_001699"
     CDS             complement(3587..4528)
                     /locus_tag="IW715_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1022305.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4654..5775
                     /locus_tag="IW715_001700"
     CDS             4654..5775
                     /locus_tag="IW715_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1022306.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            6033..7535
                     /locus_tag="IW715_001701"
     CDS             6033..7535
                     /locus_tag="IW715_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1022307.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7602..8129
                     /locus_tag="IW715_001702"
     CDS             7602..8129
                     /locus_tag="IW715_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1022308.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8340..9401
                     /locus_tag="IW715_001703"
     CDS             8340..9401
                     /locus_tag="IW715_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1022309.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9404..10300
                     /gene="murQ"
                     /locus_tag="IW715_001704"
     CDS             9404..10300
                     /gene="murQ"
                     /locus_tag="IW715_001704"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1022310.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10314..12296
                     /locus_tag="IW715_001705"
     CDS             10314..12296
                     /locus_tag="IW715_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1022311.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12361..12807)
                     /gene="nrdI"
                     /locus_tag="IW715_001706"
     CDS             complement(12361..12807)
                     /gene="nrdI"
                     /locus_tag="IW715_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1022312.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            13018..14442
                     /locus_tag="IW715_001707"
     CDS             13018..14442
                     /locus_tag="IW715_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1022313.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14623..15396)
                     /locus_tag="IW715_001708"
     CDS             complement(14623..15396)
                     /locus_tag="IW715_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1022314.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15393..16298)
                     /locus_tag="IW715_001709"
     CDS             complement(15393..16298)
                     /locus_tag="IW715_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1022315.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16452..16772)
                     /locus_tag="IW715_001710"
     CDS             complement(16452..16772)
                     /locus_tag="IW715_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1022316.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5489 a         3114 c         3496 g         4935 t
ORIGIN      
        1 ctatttttcc atttgtctct ctaatactca ggtcagaacg ctgattttac aacctcttaa
       61 taaacggtgt tcaaaagctt gcatctgcgg taataagcct aaccaaacga aaaataggag
      121 aagctatttt tgtttggctg ttcttattac ttgatgcagg gcagcttttt cacaacccct
      181 tattcacggc gttccagaag tagcttcttc ttatttcttg aagctgataa cttctggaac
      241 atcctctttt tttatttgga cggataatgg tcattccatt tctttgatca tttttttcat
      301 tttgtcgatc tcttcttctt gtgcctgaat gatttcttcc gctaattttt ttacttcagg
      361 atcttttaaa tcagctcgtt tacttgccag gatagcgata gaatgatgag gaatcatggc
      421 cttcatccaa gctgtgtcgt tgacagtagt ttggcttcgt accataaaca aggaaccggc
      481 aaaaacaaga agactagcgg ctaagatcat aacatttatc tttttgtttt catacatttt
      541 ccacatgaac aacagcatga tgactgccat gactgcaccc atcatcaacg ccatgaatac
      601 tcgtgtacga ctgaagaaga tatgatcgag cgcatagaca ttcaagtaca tcataccaaa
      661 cataactacc gttgaaacac cgatcatcaa caaaaacttc caatactttt tcatttaaaa
      721 caaactcctt tttttcattc ctttcttcta tcatgtcatg agcatggaga acaatcaatt
      781 attttgcaca gagggttttt gaacaccgtt taccaagagg tcatgagact gtttattcgg
      841 aaaaaggagt tgcaacaaga cttttgtcac aactcctttc ttcaatagct ccatctattc
      901 cacataaata aaataaaatt ttctatcgat ttcattcatt cgtcaatacg aacgcatcat
      961 tctttttttg ataactgaaa taaatgaaca ggtaaagcag gccagtcaat acagttaccc
     1021 caatggagtt cgtgaacagg ataccaagga aaatcaatcc taagacagct aatgcaaaaa
     1081 agctgattcc tttatcccat aatgttcctt ttcttagtgc cagtacaaag gatgccgcac
     1141 tgataatgat ccagatcaat aatactgtat acgaaaggga acctgccaga tagccaaata
     1201 atttgtcacc gacaaaataa gaaagagcta caccaagata aagaacactg gtacaaaata
     1261 aaacagcacg ttgcggtact tgatgcttgt ttaaaacagc tagttttcta gaggaacctt
     1321 ttcgatcctt caaacggaag aacaaaattc ttgaagatgc atacacaccc gaattgatag
     1381 atgagaacaa agcaaggaca atcacaaaat tgacgatatc cgctgcaaat gggatattca
     1441 ttttttcaaa taccatcaca aaaggactga cattcgtgcc tgctaaagta ttccaaggaa
     1501 aaagaatcaa aagcaaaaac aaaggaatga tgtaaaagga aatgattctt cccatgacac
     1561 ctttgattgc tttaggaatc gcacgtttag gatcatctgc ttcgcttacc gtgatagcaa
     1621 tcagttcaga accgccgtaa gaatagataa ctacaagcaa agaactgatg attcccttca
     1681 taccatgagg agtaaaccca ccatggtcag tcaattgctg aagggtaggg aaaacgcctg
     1741 tgcccaacat ttgctgaccg acaagataag tagcaaaaat gatcagtaag ataatgacac
     1801 tgattttagc aaaagccagc caatattctg tttctgcgaa taaccgtacc gaataaagat
     1861 tgatgatcgt agttaaaaca gccaacataa aaacaaatac ccaggctgga atgtgcggga
     1921 accacaactg caaaaaggaa gcagctgcaa cagcttcagc gatgatattg ataatccaca
     1981 tagaccagta gacccaatca gtaaaatcag cagaatgagc acctagataa gggcgtatca
     2041 aaccagataa tccgtgatgc gtgtcgccac cgcttaaaac gatttttccc agacctttca
     2101 tcacgatata taaaatcagc ccaccaatca aaaaagcaat caatacagaa gggccggcga
     2161 tcccaatcgc ttcgccactt cctttaaata gacctgcacc gattgctcca cctaaagcta
     2221 acatggtgat atgtctggag ctaagttgtt ttttcaattt tttatcttcc ataaatgacc
     2281 tctttctttt aaattcacaa tcaaaattta tattaagaat aaatagcaat atttaacaat
     2341 gactaatgat aacaaatttt cagacaatta caactattca gaaaactttt aggcgaattt
     2401 taatagaaga atgatttatt tcatcatatt ttcatacaaa tgttaaattt tcatacaaat
     2461 ataggaaacg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2521 gagaaagtga ggcggaaaag atgaacaaaa atgacgtact gctagttgca aaaaataaat
     2581 cactttattt tttgccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2641 agtcggaatt cattaaatca gctagacaac agttggcaaa ttatcagctg atcgtttttt
     2701 tagattatgg atttgatatg gagatggctc gacgcgtgcg cccatttacc aatgcaaaaa
     2761 ttgtgttatt tttttggaat cacttcaaag cagaacacga ggcgatgctg aagcaggcac
     2821 aagatgaacc ggcaatcgat gagatttatc attttgattt tttggaagca aaagaacttg
     2881 ggctgaaaca taatagttca ttttattcga aacatatgaa tttttcttct agcgaaccaa
     2941 agacggatct attttttggt gcaacgaata atgggagaaa agaacgagca gaaagctata
     3001 agcaggaatt tataaaaaga gatgttacga ccaactattt cattcttcca catcgaggga
     3061 atgaacaacc tggatatctt acttatgaag agtacctgaa aaaaacaggg gaatcgcgcg
     3121 ggattctgga actgttgcgg gagggtcagc aaggagtgac tttgcggaca tttgaatcca
     3181 tgtattttca aaagaaacta gtgacggata atcaagcgat tgctcattat catttttatc
     3241 gtcctgaaaa tatttttttg cttcgagaac gtgatttaga tgaattaccg gaatttctgc
     3301 atacaccatt taagccaatc ctatcagagc tattggaatt ttatgatgca gaaaattggg
     3361 caaaacgatt tatcactaac gattccacta tttatgagca atacgaatat gacctctcct
     3421 ctgttgaaaa caaataaaaa acctacattt tgaagagatt atgtactgaa acaaacaagc
     3481 acaattctta tggcatcatg agagttgtgt tttctttttg atcttttatt gggcttaaaa
     3541 aaagagaacc agctgctgat tcagctgatt ctcatcagag agccttttat tctgcgccaa
     3601 ggattccttc ttttgcatgg actaattggg tcaaaaaggc acaatatggt gtagcaatat
     3661 catatttttg tccttttcgc caaatggcac cattgatgta gtcgatttca gtcaaacgat
     3721 ggtttttgat caggtcttgg tacatagatg ggtaatgcaa cccgatgttg tcaggatcat
     3781 aagtagaagc aatatgttgg tacacttctt cttgatctag tatgattcct tcttttgctg
     3841 cgatagaagc aaattcagat acgattgttc gaaccatgct ctctgccgct ttttgcgcgc
     3901 ctagttcagc aatattacaa tccaaaatcg tgcataaacc attcaatgtt ccatttacac
     3961 aagcttttcg ccaaatggaa taacgaacgt tatggctata gatcggattt aaatttgctt
     4021 cttgaaagac ttctacaact ttttttgtaa atgcttcgcc ttctggttcc aagttctcta
     4081 gttcgatttc tccatcacct aacaatgtga cttttccggg accagctaga ccagctgtcc
     4141 acatcgtgat cccaaataag atatttttct ttggcacata tttttccagt acatcttcat
     4201 gacctaatcc atttagtaaa cacaaaacat aagtattgtc agtaataatg gattggatcg
     4261 aacaaaacat atcatctaat tgattggctt ttgttaatgc tacgatcaag tccacgtgtt
     4321 cgttgctttc gatgatttct tctggtgaat agattggcag ttttgcgacc acttctttac
     4381 cgttaaagtc agcaatcaac ccatttgtac gaatggcttc aatgtgagca ggccaacgat
     4441 cgatcaagag gacctcattt ccgctttgat gtagcatcaa tccgattcgg ctgcccattg
     4501 ctccggcccc agctattgcg attttcatgt tttttctctc ctttattcaa tgagttccct
     4561 tttagtatat agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa atgaaaaaaa
     4621 gtgtcttact taagaagaaa gtgagggata cagatggata ttttggcggc aattgattca
     4681 tttaaaggaa gtgcttcttc atttgaacta aatcaggcag ttctaaatgg ctgggaagat
     4741 cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac tgcagaagca
     4801 atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct cttattccgt
     4861 cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc agtcatcgaa
     4921 tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac tatccgactt
     4981 gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga agtagaacaa
     5041 attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt actccaatcc
     5101 ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac taaaaaactt
     5161 cattttgatc gaaaaaacgt tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt
     5221 accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca aaaaggagga
     5281 atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca gcaaataaaa
     5341 gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg cggtattggt
     5401 ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt cagccaaatc
     5461 actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga agggcggatc
     5521 gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc aaaaaaatat
     5581 cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg actttctgat
     5641 tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga agcaatggaa
     5701 cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag atttttcacc
     5761 atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt caaatatagg
     5821 acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct gcattcagac
     5881 tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt ggttttttcg
     5941 atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag ttctgaaaat
     6001 aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata aaaaatctga
     6061 cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta aattttgaca
     6121 cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact ttactgaata
     6181 ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa tttgcctatt
     6241 tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat gatattacgg
     6301 attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat cctgaaatct
     6361 tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta ctggcacttt
     6421 tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat ttggatatct
     6481 ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc atcgtggtca
     6541 gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc
     6601 aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa cttcgtgatg
     6661 aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta aagaaaacag
     6721 cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag aaacaagtcg
     6781 gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct gatgctgcac
     6841 ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc agcgagaaag
     6901 aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc caagcgtctc
     6961 accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat ctgttattcg
     7021 agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt cctaacggtg
     7081 caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag gttataggag
     7141 aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat gaagacaatc
     7201 gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca tttttgaaca
     7261 acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag cagatgagta
     7321 tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct gaactatata
     7381 catgggatga accattgaat tatttggatg tcttcaatca agaacaatta gaacaactga
     7441 tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc ctggataaag
     7501 tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa gaacaacaag
     7561 gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat gtaacgagac
     7621 acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat gtgccgctga
     7681 ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc
     7741 caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca caagcagttg
     7801 ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg gattttgggg
     7861 attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta gcgttcgctg
     7921 tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt cctttattga
     7981 aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat gccttgataa
     8041 gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca ttcatggtag
     8101 ataatacaga attggtttct tatgagtaat tatgtttttg tgttatttaa ataaaatatt
     8161 tcaaggcact gcttttgctt agcagtgcct tttttaggtt atgacaattc ttttgataat
     8221 ataaactcaa cctttagaaa tgaaatttaa ttattttaat aaaagtattg aaattttgtt
     8281 tctttagata tatactctag tcatagaaat gaagacggat acagaaaaag agggataata
     8341 tgtttggctt ttcaatattt atgaacgatt ctttgtcaga agagaagaaa cagtatatcc
     8401 gccaaatggc atccaatggc tttgtcggta ttttcacttc catgcatata ccagaagatg
     8461 atgtttcggc atatcaaaag cgattgatcg aactaggaag cgaagcgaaa aagaataaac
     8521 tagaattgat ggtcgatatt tcaggagagg cattggaacg agcgggtttt tcattagaag
     8581 atctaacgcc tttgaaagca attggtgtaa caggtttaag aatggattat catatttcca
     8641 atcagcagat tgccgactgg tcacatcaat tgaaaatcag tttgaatgct agcacgatca
     8701 cgcctaaaga tatcgatgaa ttgaaagaag cagaagctga tttctcacag attgaagctt
     8761 ggcataatta ttatccacgt cccgaaacag ggctggacaa agaatggtat cagaaaaaaa
     8821 atcaatggct caaaattcaa ggtctccttg tccaaggttt tgtaccagga gatacagagt
     8881 tgagaggccc actatatcaa ggattgccaa ctttggaaga acatcgaggg atccatcctc
     8941 ttgcaggcgc tctagatcta ttagcaagta acacggatat tgtttacatc ggagatgcag
     9001 gtctgtcaga aaacgttcag gagcaatttg cttctttcca aaaagaacag acagtcttgc
     9061 tccatacgga acctgtagat gaagaatttt acgaatatat attagggaag catacgaatc
     9121 ggcaagacga tgcaagagat gtcatacgaa gcgtagatgc acgttttcga gagattccgc
     9181 ctatcccagc aagaaataca gctacgcgta tgaaagggtc tattacttta gataacgaaa
     9241 agtatttgcg ctatatgggt gagatccaat taaccaaata tgatttgcca gcggatgaaa
     9301 aagtgaatgt agtagctaaa gttatcaaag aagagctacc attgatcaac caaatcaaag
     9361 ctggtatgaa ctatcaattt ataagaaaag aaggaagata aaaatgagca aaatcaattt
     9421 agagaaccta acaacggaac gcagaaacca agaaactttt ggattagatg agatgagtat
     9481 ttctcaagcc gttcaactaa tgaacaaaga agatcagaaa gtggccgctg cggttgaaaa
     9541 agaactgaca agtatcgaac cagtgattgc taacacgatt gagtccttta aaaaaggtgg
     9601 acgtctgatt tatatgggag ccggaacaag tggaagacta ggtgtgttag atgctgctga
     9661 atgcgtaccg acattcggtg tagaaccgga aatggtcata ggtctaattg ctggtggaga
     9721 agaagcaatg actgtagctg tcgaaggggc cgaagattct tatgagctag ggcaagaaga
     9781 tctaatcaag ctgaatttga caaaaaatga tatagttatt ggtatcgcag ctagcggtcg
     9841 tacaccatat gtgatcggtg gattagatta cgcaaaatcg atcggtgctg tcacaggaac
     9901 gatttcttgc aataaacaag ccgaaatcag taaacacgcg gatttcccaa tcgaagtcga
     9961 tgcaggtcca gaatttttga caggctctac tcgattgaag tcaggaacag cacaaaaact
    10021 tatcttgaat atgatttcta ctatcagtat gattggtata ggtaaagtat tcaataactt
    10081 gatggtcgat gtcaaaccaa caaatgaaaa actaatcgaa cgctcaaaac gaatcatcat
    10141 gcaagcgaca gaagctgatt acgaaacagc tgaaaagtac tttgaagaag cagatcaaaa
    10201 tgttaagctg gctatcgtca tgattttgac aaatagtgat aaagaggaag ctcaagcaaa
    10261 attagctgaa gcaaacggat ttgtaaaagg gaccttatga gggggaaaag accatggcaa
    10321 aaaacgaact gactttggaa gaactggcaa aacaaattta taaaggggca ggcggcatgg
    10381 gcaatgtaga aagcattgtc cactgcatga ctcgtgtgcg tatgaagatc atcaatgatg
    10441 atcttgtaaa tgtctctgaa ttaaaagcga ttccaggagt gcttggtgta gtagaagatg
    10501 aacagcttca agtcatcatc ggacctggga aagtgaacaa agtagcacag caaatggttg
    10561 atttggcagg cgtgaaatta ggagaaaaac tttcttctgt aagtacagct acagcaaatt
    10621 catcagcatc tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa
    10681 aatcgaaaca aaaaccatct aaaatcaaat cggtattaaa agacatttct aatatctttg
    10741 tgccaatgat tcctgcattt gtcggtactg ggatcgtggc agggattgcc gcagttttag
    10801 caaacttagt cacagccgga acacttgatg cagcaacatg gcaacaatat atcgatgtga
    10861 tgaacatctt gaaaaacggg atgtttagct acctagttat ctatacaggt atcaatgctg
    10921 cacaagtttt tggtgcaaca ccaactcttg gtggagttat cggggccgtt gtgatgctga
    10981 cagggatgaa tccggaagca ccactgaaaa acttatttac tggtgaagct cttgcagctg
    11041 gacaaggtgg gatcctaggt gtcatcttcg ctgtttggct attgtcaatc gttgagaaaa
    11101 aactgcataa agtagtacct gatgcggtag atatcatcgt tacaccaact ctttcattga
    11161 ttgccatcgg tctgattgaa attttcttca tcatgccttt agcaggtttc atttctgatg
    11221 gcatggtcgg tgggatcaac tgggtacttg gcgttggtgg agcatttgcc ggcttcgttc
    11281 taggcacatt attcttgccg atggttatgt tcggactgca tcaaattttg actccaatcc
    11341 atgtgcaaat gattgatgaa actggtcgta cgctattgct tccaattctt gcgatggctg
    11401 gtgcaggaca agtgggtgca gcattggcat tatggattcg ctgcaaaaaa gataaagaat
    11461 taacagaaat gatcaaagga gctttaccag tcgggatcct aggaatcggc gaaccattga
    11521 tttacggagt aacattacct ctaggacgcc catttattac tgcttgtatc ggtggaggta
    11581 tcggcggggc agtgatcgga atgatcggaa atgtcggggc tatcgctatt ggaccttcag
    11641 gagccgcttt gatcccattg atctctgacg gtaaatggtt aggctacatc cttggattat
    11701 tggcagctta tgctggtggc ttcgttgcta cattcttctt cggtattcct aaagaccgtt
    11761 tagaaaaaga agaattagcg actgaaacag ccacagtaaa tgaagtgcct gaaaaagtag
    11821 tacctcaaac ttcagctgaa agtgtgacta tcctctcatc agttgctgac ggaacagttg
    11881 tttctttaga taaagcaagt gatccagttt ttgcacaaaa gatgatgggt gaaggctact
    11941 ttgtagaacc agaaaacgga catatctatt ctccagttac tggggtagtc agctctgtct
    12001 tcccaacgaa acacgctatt gggatcacaa cgccaaatgg tttagaaatc ttgcttcata
    12061 tgggaatcaa cacagttgac ttaggcggta cgccatttga tgtgaaagta acagaagggc
    12121 agcaagtgac acctgatacg cttgtagcag aagctgactt agaagcgatc aaagcagcag
    12181 gcaaagaaac atccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct
    12241 tggaaaaaac aggtaaagca aaagcaaaag cacctgtgat ggatgtcaaa gcttaatatg
    12301 atagaaacaa tagaaacgaa agaagaaact gaaatctatt tttcagcttc ttctttttta
    12361 ttatttattg ttttctgcta aaatctgata aattcgtgtc acatcagatg gtgttccccg
    12421 taattcataa tctgccaaga aaggaaagcc aaatgtttgt gcatattgtt tggctgttag
    12481 acaatactga ttattgaagt ttttatttcc gctaccgact acaccaagac aaagtttatg
    12541 attgtcttca tgttccagat attcacgcat cgtttcggtc aagatttctg tatcaccgtt
    12601 atctaaacca tttccgccgt ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc
    12661 tttcgtaaaa tcactatttt catggatttc ttttaaagta acttcaggat tgacaggatc
    12721 ttctgcatgc attttttcag catattctgc tagatgtttg gcaaaggcac gcgtatttcc
    12781 tgaaatcgat atatataaaa tgttcatgga tcatcctcca attctttcct tttagtatag
    12841 catctttttt aagttttttg gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat
    12901 aatgagaaaa aagccggtag aattatattt ctattaaagt ttttattaat taagtaaaaa
    12961 taaaaaaggt ttgttatact ttttgataaa agcggtttta ttttggagga tgcttacatg
    13021 gatacagagc aacagaaaaa aggaattggt ttaattccac ttgctgcctt agtaattggt
    13081 tcagcgattg gaggtggcgt atttggtatt atgacggaca tctccggatc ggctggcggc
    13141 ccagctattt tcagttgggt actcgtagga acagcaatgt tgatgctctc cttgtcgatc
    13201 aataatatca ataaaaaacg tccagattta aaaggcggga tctttagtta cgcagaagaa
    13261 ggttttggcc gtttcgctgg ttttatcagc ggttggggtt actggctcac atgctggtta
    13321 ggcaacgtag cttttgcaac attgttgatg agcgcattag gatatttctt cccagtattt
    13381 gaaggtgggc aaaatctggc atcgatcgtg atgagtacag tctttttatg gggatatgca
    13441 tggctagtga atcggggagt cgaaaatgct agtttcgtca atgctatcgt cacattctgt
    13501 aaattagtgc cattattttt atttattgta gtagctatca gcacgttttc gatccaacaa
    13561 ttcattgata acttttctag cgacattata ttgaatgcga atggacagcc gatcccgttt
    13621 tccagtcaag tattgagttg catcatggtg atgctttggg tatttgttgg tattgaagga
    13681 gcctctgtcg taggttcacg agcaaaaaaa caatcagatg ttgggaaagc aacgattcta
    13741 gggatattcg gcttgattat tatttacgtc ttggtttcca tgcttccata tgggacaatg
    13801 acgatgaatc agctgcaaac gatcaaggcg cagcctgcta tgggctatgt gtttgaaatg
    13861 atggttggta aatggggagc agtcgtgatc aacggcggtt tgatcatttc tttagttggt
    13921 gtttggctct catggacgat cttaccgatt gaaacaatgg gaaacatggc tgaagacgga
    13981 cttttgccta aagcatgggg aaaactcaac aaaaatgggg caccaactta tgctatcgta
    14041 ttgacgacgt tatgtacgaa tgtcttttta ctttcattat tattcactga cagtgcttat
    14101 aactttgctt acacacttgg tacagcagct attttcttca cgtggctttt cttaggattg
    14161 taccaaatga aactttctta tcagcggaaa gaatgggtcc aatttttagt agggctttta
    14221 gcaagtgcct ttcaagtatg ggctatgcta gtcgtagcct ttaatgaagt aatgcttgtt
    14281 cttgttcttt tcttgccagg catttatttt tacctcaaag cacgtaagga gcaaaacgag
    14341 cctgtacaac atatccactt agataagatt ttacttatag gtgtgacgat ggtcggagtg
    14401 attgcaatgg ttttatttgc cactgggatg cttcacgtat agaagtataa aagaagtata
    14461 aagagaggtt gtgagcctgt cgtttattca aaaacatact aggaaaggag ctgtgacaca
    14521 aatgttgtca cagctccttt ttcagaataa acggtgttta agagacagcc aatcggcaat
    14581 aaggctgaac gtctctttca cagcctcttt tttgatgagt catcatacat tttttcgctt
    14641 aaatacgaga tatcctaatg ctaagaacaa aacgatgtaa gctaaattac cgaatagcat
    14701 ttgattagta gaaagatgag tcaattgctt catgatctct tcgtttccaa tttggttttg
    14761 cagattcaac atactgatcg gattccattt gatccactcc catttttgga tgagtgcaat
    14821 aaggatacca gatatgattg acgaagcaaa ataaaagacg attcctgctg agatggatgc
    14881 gccggaacta ttgataaagc aagcaatcat caacactaaa cttaaaatca gccataaacc
    14941 gatataacta cccaaagtat aagtgaacaa gctttggatc agtgtttgac cagtagaaac
    15001 cttctccgtg aaggaaatcg aagggaaaag aatcagtttc atcaacacag tgacaataat
    15061 agctaaaagg tataaataaa cagaataaat aactaatgtg atccatttgc ttactaatat
    15121 ttctcctcga gaatactttc gataaaggag atttttcaat gtaccatatt gtgcttccat
    15181 cgaaatgatc gtgcttgcag ctgcaatcat gataaagaca atccaagatg tggcactaaa
    15241 aagctgagca gtcatttcaa ctggatcaat gatcgtcgtg tattttttgg ctagaagtgc
    15301 tgttcctatg agcaaaacaa ctaatagaac agataaaaca gcagttgact ttttcttgat
    15361 caatttaaat aattcttgtt ttacaagcaa tgtcattttt cttcttcccc ctttactgaa
    15421 tccagaattt ccaatagaga agattccaaa tcattcgtca gatgctggac gtctgtcaca
    15481 tggatatttt gtgcagacaa tagctggatc acggttccta ataattcttc gttttctttt
    15541 aaaagcttga ttttatcagg atcttccaaa gtatatccag cttctgtcaa gatcgttcga
    15601 gcagctagat catttgttgt agagataacg atgaaatctt gcgctgtttg gttcaattca
    15661 gccattgtac attgtttgat gatctttcct tgattgatca ggatcagctc atcgaccatt
    15721 ttttcaagct cgctgagtaa gtgactggag atcaagaaag aggtgccctg agtagccaag
    15781 tcggtaatga tttttcgcac atctcttgtt gcttgtggat ccaaaccgtt catcggctca
    15841 tctaaaataa cgatagacgg atggttcagt aaggcaagag caattcccaa tttttgtttc
    15901 attcccaacg agtaggattt cgcttttttg tgaatgtact tttccatctt gagctgattc
    15961 acgatagtgt cgatagcaga tacatcgttg gtttcagaga ataacttcag atgatcatag
    16021 cctgttaaga atggatagat acccgggtat tcgatcaatg ctcctacttg ttccaaccct
    16081 ttatgactag agggagaaat ctcattttca ccaatacaga ttgttccatc tgagtaatgg
    16141 atcagtccta aaatagcttt catgatcgtt gtttttccag cgccattagg acctactaat
    16201 ccaacgattt tgccagggga taagctaaaa ctgacatcgc tgagggcttg tttgtgacca
    16261 aagttcttgg ataattcttg gaccgaaaga cttttcaaaa atattcactc cttttctata
    16321 gatagtacaa gcatacacct gatgagtgga gaagtcgcta ggtcattgga cttatttctt
    16381 attttttcat aaaaaaattg aatattaaat aaaaaggctt attttttctg atcagtactt
    16441 attccaagtg tctagtggcc gctagtaatc ttgatgccaa tgatactgat gagtagcatc
    16501 cccacaaaga accaagtgga aagattcagc ttatcatgaa acaagaccac accaattatg
    16561 accgaaccaa ctgcgccaat ccctgtccag ataggataag agagactcat cggcaaactt
    16621 ttgattgcct ttgacaaaaa gtaaaaacta gcaatcatgc caatcactgt gtaaaaagaa
    16681 taattgattt tagaaaaacc atcactccat ttcatcatcg ttgaccagaa tacttccaaa
    16741 attcctgcta tgattaattc catccatgcc atacttacat tcctccttaa ctataaaaaa
    16801 atacacaaaa agaggaaaat catcccttca aagacctaat ggaatgatca tcctctttat
    16861 cctacccggt ggcagattgt atatcttttt ctgtagtata gcacacctat ccagatatcg
    16921 gcaatctcat actttttagt aaagtgtacc aagcactgac ggatatatcg ttagcggaaa
    16981 agaagatgaa tgctttgcaa tttcatagaa agaagttgtt atttattaat catt
//
LOCUS       DACEMK010000057        16595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEMK010000057 DACEMK010000000
VERSION     DACEMK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.059"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..780)
                     /locus_tag="IW715_001711"
     CDS             complement(46..780)
                     /locus_tag="IW715_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1022317.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IW715_001712"
     CDS             1106..1516
                     /locus_tag="IW715_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1022318.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IW715_001713"
     CDS             1509..2210
                     /locus_tag="IW715_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1022319.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3823
                     /locus_tag="IW715_001714"
     CDS             2210..3823
                     /locus_tag="IW715_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1022320.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3813..4034
                     /locus_tag="IW715_001715"
     CDS             3813..4034
                     /locus_tag="IW715_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1022321.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4031..4792
                     /locus_tag="IW715_001716"
     CDS             4031..4792
                     /locus_tag="IW715_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1022322.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5173..5682
                     /locus_tag="IW715_001717"
     CDS             5173..5682
                     /locus_tag="IW715_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1022323.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5752..6291)
                     /locus_tag="IW715_001718"
     CDS             complement(5752..6291)
                     /locus_tag="IW715_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1022324.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="IW715_001719"
     CDS             complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="IW715_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1022325.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7358..8155
                     /locus_tag="IW715_001720"
     CDS             7358..8155
                     /locus_tag="IW715_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1022326.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8183..8818
                     /locus_tag="IW715_001721"
     CDS             8183..8818
                     /locus_tag="IW715_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1022327.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8838..9611
                     /locus_tag="IW715_001722"
     CDS             8838..9611
                     /locus_tag="IW715_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1022328.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10019..10816
                     /locus_tag="IW715_001723"
     CDS             10019..10816
                     /locus_tag="IW715_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1022329.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10794..11207
                     /locus_tag="IW715_001724"
     CDS             10794..11207
                     /locus_tag="IW715_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022330.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11191..13836
                     /gene="mutS"
                     /locus_tag="IW715_001725"
     CDS             11191..13836
                     /gene="mutS"
                     /locus_tag="IW715_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1022331.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13854..15962
                     /gene="mutL"
                     /locus_tag="IW715_001726"
     CDS             13854..15962
                     /gene="mutL"
                     /locus_tag="IW715_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1022332.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15984..16544
                     /locus_tag="IW715_001727"
     CDS             15984..16544
                     /locus_tag="IW715_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1022333.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5387 a         2965 c         3599 g         4644 t
ORIGIN      
        1 cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag ccataaataa
       61 taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa ctggtaccca
      121 tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt gtacaaaacg
      181 tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa gtgaagcaac
      241 taatgtccat acaaggaaac cgagtgctaa gtgagctgta ccaagattat cttggaagaa
      301 tggtgctttt gtaataccta aggcaccaaa gaataaaacg aatgaaccaa agaattcatt
      361 gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac ctaacacatg
      421 gttcacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga tcaattgtcc
      481 aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc aagggaatag
      541 accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc tgattccacc
      601 aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca gcatccagtc
      661 actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt tacctagaac
      721 gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg agtaatccat
      781 aaatacctcc aaaagttata aaaatgcaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaacca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagctatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agagggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc aggaagggta tctgtaaacg aaatggagga
     1501 aaatcaaatt gactgagttt tttgtgaatc cactatttgg cttaagtttg actttaggag
     1561 tgtatgtatt atctgataaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat cggtacagca aaagcaatcg
     2101 agattggcga aatagaagga atcatttccg gacttgcgat atgcgtaaat gggatattga
     2161 ccgttttgtt gctgccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatacatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaagtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg ggtcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgaccgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaaaaaac
     3781 tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt ttaccagaat
     3841 tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt tatgttgttc
     3901 tcgtatccga tgatgaattt gatggctgtc cagtgacacc ttacttattg atcaaaacaa
     3961 gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca gatgattggg
     4021 cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat ttatcacagg
     4081 agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag atgcttttgt
     4141 atttggatta gatcaaaagt tcggtaaaat ggaagaactc tctttgcgat ttcctgaaac
     4201 ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag cggtatcgaa
     4261 atgccaggat cgttttggtg aagttacgat tttactgaat acatcgggga tattggatgc
     4321 ctacaaacca ttgatggaaa ctgacgaatc gctgtgggat cttatttatg agacgaatgt
     4381 gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc gaaaaagcgg
     4441 aacaatcatc aacatggctt cgattgcggg tttggttgct ggcggaggtg ggatcgctta
     4501 tacaagtgcg aaacatgcaa tcgtcggatt taccaaacaa ctcgctctgg attacgcagg
     4561 acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa tgaatgcggc
     4621 tgattttgaa gggaaaggtg agatggctga gtgggtcgca aatgaaacac cagtcaagcg
     4681 atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc ctcaagcaag
     4741 ctacatacaa ggaacgatca tcccaatcga tggcggatgg ttgttaaaat aaccttgtgt
     4801 ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat gataaaagaa
     4861 agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat tctttttaat
     4921 aatcgttaat attcgaaact aagcggcttt tcacgacctt ttgatataaa cggtgttcaa
     4981 aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc tatttttatt
     5041 tgactattct tattacttgt tgcagggcag ctttttcact accttctgcg gcacttcagt
     5101 tggaattttc ttcccatcct ttggtcgcag tcattaccaa gataaaatta tcttgcaaag
     5161 gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac aacgacagat
     5221 gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt tttgtctgtc
     5281 tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct gtccttgtat
     5341 aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat ggcttctcct
     5401 cttggactga tagatgttgt agtaggaagc gtcagcacgc tgcttgtatt gcttatcaac
     5461 tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggctgtgac agcatgcgta
     5521 tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca actacctttt
     5581 ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt cggtgggata
     5641 attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt ttaatttaaa
     5701 atatggcagc gtggaagaga ctttgagtct tcttccatgc tgcttttttg attaatttgt
     5761 atgaagaaat cgatttagcc gttttttcat caagataccg atataagctg ctccagttgc
     5821 cttgattgct tccggcagta aaaacggcag tagtccactg gcaaatgcag ttgtaaaagt
     5881 aagatcacca cttattttca gccagatcgt tccgcagatc aatgcagcaa gagagcccat
     5941 tagattggca aagattgcat ataagtaatg agcatccgtt ctttcgatca tccaaccagt
     6001 tatccacgtt tgtaagagaa atccaatcaa atatccgcca gttggcccaa ataaaacagc
     6061 tatacctcca gtcatccctg aaaatacagg aagaccaatc aagccaagta aaagataaac
     6121 tacaacagat atcaatccta gttttcttcc gagaaaagtt gctgttaatc caatagcaaa
     6181 tgtttgcccc gtcaaaggaa ccaagccaaa aggaatggtc atctgagaaa ggacagaaat
     6241 aatcgcggca aattccgcag caagtattaa ttctttcaat gttcggttca taatgccctc
     6301 ttttcgttaa cttaatattt ttatttggtt aacgaaattg taaaacaaag tgattacttt
     6361 gtaaatagaa aatataaaaa aagaccagct atccaataga tagcggtctt tttcaagaaa
     6421 gcttttgaaa ttataagctt tggttgtagt attcaacgac aagagcttcg tcgatttctg
     6481 gatatagttc gtcacgttct ggtaaacgag tcaatgaacc ttccaatttt tcagcgtcaa
     6541 agcttacgaa tgctgggcgt ccaacagttg cttctacagc ttcttttact gttacgatgt
     6601 tttgtgattt ttcacgaact gagatcactt gacccacttc tacgtggtat gaagggatat
     6661 caacgcgttt gccatcaact aaaatgtgac catggttaac taattgacgt gcttgacgac
     6721 gagtcgttgc aagtcctaaa cggtaaacaa cattgtctaa acgttgttct agtaagatca
     6781 tgaagttaac accatgttta ccttctttga ttttgctagc ttttacgaac aagttacgga
     6841 attgacgttc gttcattccg tacatgtgac gaagtttttg tttttcagtt aattgcatgc
     6901 cgtattctga ttgtttgcca cggctgttag gtccatgttg acctggtgcg taagggcgac
     6961 gtgctaattc ttttcctgtg cctgacaatg agatgcctaa gcgacgagaa attttccatg
     7021 atggtcctgt ataacgagac attaaaattt cctccaatga atataagttt ttggagtaaa
     7081 ataatccgtt gaaaaattca taatcatata gtttgttctt caatcttcac ctttgcagcc
     7141 gcggttacac aatcgaaccc gagacggggc aacaaactag ggatgagtta ttatttttct
     7201 gctgcattat tttacacaaa ctacagtata tgatagatca tccacgaatg tcaagataat
     7261 tagtacataa aaacgcttta caaacctagg ctgtctttat aaaatgagag ggcatgtgca
     7321 aatggaaaaa agcgaaggaa gtttaaggag aaaatcaatg aatccggtat caccattatt
     7381 tgagaaaatc gatgcttcaa ttgaaaagaa ggcgaatctt tttgaaaaca gcttttcacg
     7441 ttacgctgtt cgagcggttc tcgcctgttt gtttttgact ttaggaacgg ctgtggcatt
     7501 tgcaatcgcg atgaaggggg aagatatcgc gcatggtcta ggaaagattt tatacgcatt
     7561 tatgttcagc tggggtctgg tcatgattct atacatgaac gctgaattag gaacatcgaa
     7621 tatgctttat atgaccgtgg gtgtttttcg gcagagactt gctttaagta aggcagccaa
     7681 gattctattt gcctgtattt ttttcaatat gattggcggt atattatgtg gatttcttat
     7741 ttctatgact acgccatttc aagatttaac gttggacaac tttatgttag aatcaatagc
     7801 agtaaaacta gcaaaacctg cagcaacgat tttggtggaa gggatgtttg cgaatatcgt
     7861 tgtcaatacc gctgtactga tcagcatgcg gatgaaagag gatgccggga aagtgatcac
     7921 agtcgtattc atcattttca tttttgcttt tttaggatat gaacacgtga tagcgaattt
     7981 tcctgccttt acgcttgctt actttgcttc tcaaggacag acggatggaa tgacagtcag
     8041 caatgtgtta cataatttgt tttttgcttt ggcaggaaat tatattggtg gcggtttagt
     8101 aatgggactg ggatatgctt ggctgaatca gtcaaaatca tcctatgtgg actaaaaagt
     8161 cgttcattaa aggggaagtg ccatggaagt tttagttttt ccaaaaaatg aggaaaaagc
     8221 gttacaaatg gaaaagtaca tgaaaaatct tttttccttt gctggagtac ctaagccgga
     8281 acgcgctctt ttacaacaac cgttactgga tgagagcaaa aatttatcta tttctgaagt
     8341 tttagctttt gtttattatt attatgaaca aatcgaaaga gagtatcagt atgtagcgat
     8401 cgatttagtc cagaaaaaca tcaaacggct tacatttgaa gacttagtcg tattgttgcc
     8461 actagttcag aaaaaagaat ggtgggacag catcgactct tggagaaaag tatacagcac
     8521 atggtgtaaa gcctacccag agcagcgtga agaagtcttt caattgtttg aaaaaaagga
     8581 agatttttgg ctacgtcgaa tagcaatcac gttgcaatta ggttataaac agcaaacaga
     8641 cacaggacta ttgatccgag caatagacga agatcgtcaa acaaaggaat tctttatcca
     8701 aaaagcaatt ggttgggcat tacgcgatta cagtaagacc gatgccgatt gggtaaaaag
     8761 acagctgaca acagatttga gtaagttagc tgttcgagag gcaagtaagt atttataaga
     8821 aagcgagacg acaagagatg agacgcaaat tatttgcttt tgatattgat ggaacgttgt
     8881 tagattcaaa caaacaagct ttagatagca cgagagaagc attggaaaaa ttacgcaaag
     8941 ccggtcatct ggtcacgatt gctacagggc ggagccgatt tcatgcgcaa gagatcattc
     9001 gcgatcttga ttttacgaac tacattttat gtaatggagc ggccggtttt ttagaccatg
     9061 aacaagtata taagaattta ctagatcgcg accagttgga tgcgttcgtt caagaagcgc
     9121 atcatgagca gatcgataca gcttttgtca gcttagacaa tatcaagcgt tcttcttcca
     9181 atgatgtaac ggtgatggaa gaagcgatgc attctttcgg tgctgtcttg cctgaattag
     9241 atcagttttt tgcagaggaa caagaaatat accaagcgtt agcatttttc gatcaaacat
     9301 atgatgggaa gttctcttca tatgataaaa ttcgatttgt ccgctggcat gagaacagtg
     9361 tagatgtcgt acctaaaaat ggttctaaag cagcgacaat tctgcactta gcagaacaag
     9421 taggcatccc tcatgaagac atcatcagct ttggcgatgg acaaaacgac cgtgaaatgt
     9481 tgcgcatgtc aggaattggg atagctatgg ggaatgctgt gccagaagta caagcagaag
     9541 ctaagatggt cactgataca aacgatcagg atggaatttg gaaggcattg aaagagttaa
     9601 gcgtgattta aaagaggttg tgaaaaagct gttctgcatc aagtaataag cccagccaaa
     9661 caaaaatagc tcctcctatt tttcgttttt ctgggcttat taccgcagat gcaagctttt
     9721 gaacaccgtt taaggaagag gtcgtgacag aagtggtcag tttcaagaaa taagaaggga
     9781 tttacgaaaa ttgcttctca aatttttgta aaattacggc ttatttctga agaagtagat
     9841 gaaacaacgt ttattcggga aatgggactg tgacaagatg tttgtcatag tcccattttt
     9901 ttattttatt tttcaaaact aagaaatacc aggaaagagt tccttttgtt tgaccttttc
     9961 tttcttattg aaaacaacta taatatagag tgatgttaaa agagaagagg aatttagtgt
    10021 gcgggtattg tttattggtg atgtagtagg ttcaatggga cgagagatga ttactgaata
    10081 tcttcctaga ttgaagaaga agtaccgtcc gcaagtaacg attgtaaatg gtgagaatgc
    10141 cgcttctgga cgtggaatca cagaaaaaat ctataaaaaa tttttgcaag acggcgtaga
    10201 tgtcgtgacg atggggaatc atacgtggga caaccgcgga atctttgagt ttgtaaatga
    10261 agctaaaaag atggttcgtc cggcaaattt tccggaaggg acgcctggac aagggatggt
    10321 ctttgtcaaa gtgaaccaag tcgaactggc agtgatcaac atgcaggctc gttcttttat
    10381 ggtggacttg gatgatcctt ttcgtaaaat gaatgagctg gtagaagaag ccagaaaacg
    10441 tacgccgatc atttttgtag attttcatgg agaaacaaca agtgaaaaac aagcaatggg
    10501 ctggttttta gatgggaaag tttcagcagt ggtcggtaca catacacacg ttcagacaaa
    10561 tgatgcgcga attttgccaa gaggaacagc gtatctgaca gacgttggga tgacaggtcc
    10621 ctatgatggt attttgggta tgcgcagaga acctgtaatc gaaaagtttc tgaccgcatt
    10681 accaaaacgt tttgaagttg tggaacaagg ccgatcgatt ttaagcggct gtatcttgga
    10741 attggatgat acgacaggtc atgcaaaaaa aatccagctg attcagatca acgatgatcg
    10801 accgttcatg gagtagaaag agaggcagtt ttttgcagca ggagccggaa attcaaaaag
    10861 aagtaaggaa actgacaaaa cttttaagag agaatgaaac gatcattcgc tacaaagaac
    10921 ttgaagaaaa gatccagcaa aaccagtatt tagctgagtt gagagaaaaa atcaaacaag
    10981 cgcaaaaaga tgctgttcat tttgcccact atgacaaacc agcagcagaa caagaagcaa
    11041 tcaaacaagc ggatcaattc atgcaagaat ttgatcagca tccgcttgtt gtagcttata
    11101 gaaaacagct gttggaagca gatgatctgc ttcatcattt aacaactatg atacaagaag
    11161 aaatcaatgg acaaatagag gaggaaaaac atgcctcaaa aaactaaaaa tactcctatg
    11221 atggaacaat atctaagcat caaggcccaa taccaagatg cttttttgtt ctatcgttta
    11281 ggagactttt atgaactatt ttacgaagat gcgatcaaag cgtcacagct tttggaatta
    11341 acgttgacga gcagaaatcg aaatgcagaa gaacctattc ctatgtgcgg tgtacctcac
    11401 catgcagcac aaggatatat cgatgccttg attgaaaaag gctataaagt agcgatttgc
    11461 gagcaggtag aagatccaaa gacgactaaa gggatggtca agcgcgaagt gatccagcta
    11521 gtcactcctg gtacagtcat ggacagcaaa ggattgtcgg caaaagacaa caatttttta
    11581 acagctgtag ttagtcaagg aaatgaattt ggctttgctt atgccgattt aagtacggga
    11641 gaattgaaga cggctcggtt gcatgatgaa gaagctgttt taaatgaagc ctctgcattg
    11701 cagacaaaag aactcgtatt aggcagtgag atcactgata cccttcgtga acagctaagc
    11761 gggcgtttag ggctagtctt ttctcaacaa aatgagatgg aagaaaacgc ggaatttagc
    11821 tttttgacaa gcgaactagt cgatccgctg aaaaaagaag caaccggcaa actattgact
    11881 tatttatcgg tgacacaaaa gcggagttta gctcatatcc aaaaagcagt agaataccaa
    11941 ccagatcatt ttttgaaaat ggattattat tcaaaattca acctagaatt aagccaatct
    12001 attcggactg ggaaaaagca cggcactctt ctatggttgt tagatgaaac aaaaacagca
    12061 atgggtggcc gtttactaaa acaatggcta gaccgtccgc ttatccaggc aaaacaaatt
    12121 aaagcaagac aagaaatggt cgcttctttg ttagacagct attttgaacg tatcgatttg
    12181 cagtcagcat taacgaaagt ctatgatctg gaacgcctag caggtcgagt agctttcggt
    12241 aatgtcaatg ggcgagactt gatccagctg aaaaattctt tagaacaagt gccaatgatc
    12301 agagggttga ttgaaggaat cgatcaaggt caatggcaaa gtctgctttc agaaatgcag
    12361 ccgatggacc atctcgtcca attgatcgac caagctatcc aagatgatcc gccgttacaa
    12421 ataacggaag gaaacatcat caaagatgga ttcaatgagc agctcgatat gtaccgttct
    12481 gctatgagaa acggaaagaa atggttggca gaacttgaag caaaagagcg tcaagaaaca
    12541 ggaatcaaaa acttaaaaat cggatttaac cgcgttttcg gttattatat agaaatcaca
    12601 aaagcaaatc ttggaaatgc agaactggag aagtatgaac gaaaacaaac tttagcaaat
    12661 gcagagcgtt tcatcacacc agaactaaaa gaattggaga cacaaatcct cgaagcagaa
    12721 gaaaaatctg ttgatttaga atatcagttg tttttagctg tgagagaaga agtcaaaaag
    12781 gctatccagc cgctacaagt acttgcaaaa gcaatcagtg cagtagatgt gctacaaagt
    12841 tttgcaacga tcagcgaacg ctatcagtat gttcggccag aacttgtcag tgacaagcat
    12901 cagcttctga tcaaagacgg acgacatcct gtagtagaaa aagtattagg acatcaggaa
    12961 tatatcccta acagcgtaga aatggcagaa gacgaaatga tcttgctgat tacaggaccg
    13021 aatatgtctg gtaaaagtac gtatatgcga cagttggcat taactgttct gatggcgcaa
    13081 atgggctgtt tcgttccggc aaaacaagca gttttaccta tttttgatcg gatttttacc
    13141 cgaattggtg caagtgacga cttaattgct ggacaaagta cttttatggt agagatgatg
    13201 gaagccaatc aagccctgcg ttatgctacg ccaaacagtt tgattttgtt tgacgaacta
    13261 gggcgaggaa cagcaaccta tgacggaatg gcgttggccc aagcaatcat cgaatatatc
    13321 catcgtcatg tacaagcaaa aacactcttt tctactcatt atcatgaact gacggtactt
    13381 gagaaagaac ttccgcagct taaaaatgtc catgttggag ccgttgaaaa agacggagaa
    13441 gttgtcttct tgcataaaat gatggatgga cctgcggata aaagctacgg catacatgtg
    13501 gcaaagatcg caggattgcc ttccaatcta ctagaacgag cagcagatat cctgcatacg
    13561 ctagaaaatg gcgataacgg acaccatact gttccagctg aagtagaaga gaaaactaaa
    13621 acagccaaac aggagcaaat aaaaccacct gcagaaaaag taaatcaaga ccatatacaa
    13681 gaccgtgtaa aagaagaaaa ccaacagctt tctttattca gtgaattatc cgaaaatgaa
    13741 acagaagtca ttgaatcact gaagcaaata aatttactag aaatgacgcc aatggatgca
    13801 ctaaacacat tatatgaatt acaaaaacaa ctgtaggaag aaggcggata tcgatggcca
    13861 aaatccaaga attatctgaa cgtttagcta atcagatcgc tgcgggagaa gtagtggagc
    13921 gaccagcttc cgttgtgaaa gaattagtgg aaaatgcaat tgacgccggc agtactcaga
    13981 ttgatatctt agttgaagaa gcgggactgc ggacgatcca agtgattgat aacggagaag
    14041 gcattttggc agacgacgta gagaatgcct tcaagcggca tgcgacaagt aagatccata
    14101 atcgcgacga tctttttcgc atacgaacgc tgggctttcg aggggaagct ttgccaagta
    14161 ttgcctctgt ttctgaaatg actctggaaa cggcgaatca ggaagaaaaa caaggcacct
    14221 atctgtcgct gaaaggcgga gaagttattg agcatcgacc agcaccactg cgtcaaggaa
    14281 cgaagattac cgtcagcaat ctgtttttca atacgccagc ccgcttgaaa tacgtgaaaa
    14341 cactgcacac agaattagcg aacatcgggg acatcgtcaa tcgactggca ttgagtcatc
    14401 catcgattgc ttttcgttta gtacatgatg gaaacaaaat gctggcaact gctggaaatg
    14461 gcgatctgaa acaaacaatt gcgggaatct acggattatc taccgcaaaa aaaatgctga
    14521 aaatcgaagg aaaagatcta gattttgagt tatttggcta tgtttcattg cctgaggtga
    14581 ctcgtgccag taggaattac ttatcgacga tcatcaatgg acgttttatc aagaactttt
    14641 ccttgaataa agcaatcgtt gcaggttatg ggtcaaaatt aatggtcggt cgtttcccta
    14701 ttgctgtatt ggaaatcaaa atggacccgc ttttagtaga tgtaaatgtt catccgacga
    14761 agcaggaagt ccggctgtcg aaggaaaaag aactaacaga gctaattagt aacgccattc
    14821 aaaaagcatt gtctcaagaa aatctgattc caagtgcagc ggataatcta cggtttaaga
    14881 aaaaagtacc tgatcagcct aaaacagaac aaatcgaaat cgatttgtcc tacgcttttg
    14941 aagaagaaaa acctaaaaag ccaagtagtt tgaattttga tcgagagaca ggtacctttt
    15001 tcgtagaaga accaaagaac acatttgttg acagaattgt tgataagtct gtggaaaatg
    15061 ttgaaaacta ttctggtttt tctgaagaaa acaccactta tccagaaaaa acagcgaaaa
    15121 cggtggataa gtttggtgaa aatgttgata actttgtaga aaaaacagtg gaaaaatctg
    15181 aaaagaaaga atttcccgtt gtggataaca tggagaaaac aaatgaagaa caagcagata
    15241 aatccgtaga agaggatgaa aagatttacg aggaagaatt gacactccat cctgaatttg
    15301 atttcactca aggaaacgac aaaacattag aacaagccat caagaaatta gaaacggaaa
    15361 aaccagtaca acgttttcct cagttggaat attttggaca gatgcacgga acgtatcttt
    15421 ttgcccaaag tagcgatggg ttgtacatca ttgatcagca tgctgcccaa gagcgcatta
    15481 agtatgaata tttccgggaa aaaattgggg aagtatctga tgatctgcaa gaattgcttg
    15541 ttccatttgt gttagactat ccaaacagtg atgctttgaa attaaaagag caaaaacact
    15601 tgctagaaga tgcagggatt tatttagaag aatttggtca aaacagcttt attgtacggg
    15661 cccatcctac ttggtatccc aatggagaag aagaatcgat tattcgggag atgatcgata
    15721 tgctgctgac aactggaagt gtgagtgtaa aaaaattccg tgaagcaaca gcaatcatga
    15781 tgagttgcaa acgatcgatt aaagccaacc attatttgaa tgaagcacaa gcacgtgtgc
    15841 tgctgaagga tctggcacga tgcgaaaatc ctttcaattg tccccatggc cgtccagtat
    15901 tgattcactt taccaattcg gatatggaaa ggatgttcaa acgaatccaa gacccgcatt
    15961 aagtcaaaga gaagagggaa aacatgaaga ttgtattagc atcccaatca ccaagaagaa
    16021 aagagttatt ggctcatatc gtttcagaat tcgagattga acctgctgat atcgatgaaa
    16081 cgccatttcc agacgaacag cccgtcgatt atgttcgtag aatggctgaa gaaaaagcac
    16141 gaattgtctg ggaacaagat gaaaaagaag accatttagt gattgccagt gacacgactg
    16201 ttgttctcaa tcaagagatc atgggtaaac cagaagatct ggctgaggct gaattcatgc
    16261 tgaaaaaact aagcggggaa acacatactg tatatacagc tgttgtttta aaaacaaagg
    16321 aacgagaaga acggatacta gcagaagcac atgtcacttt ttatcctttg acagacgaag
    16381 agatcaaacg ctacttggag acgggggatt atgcggataa ggcaggggca tacggcatcc
    16441 aaaatgctgc gagtgtattc gtcaaagaaa tttctggaga ttattacagt atcgtaggat
    16501 ttccgattgg tgctgtcaat caggcactga aaacattcat ttaaacaaaa aagcggacta
    16561 tctgattttc gattgaaaat ccatgatagc ccgtt
//
LOCUS       DACEMK010000058        16528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEMK010000058 DACEMK010000000
VERSION     DACEMK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.060"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..417)
                     /locus_tag="IW715_001728"
     CDS             complement(142..417)
                     /locus_tag="IW715_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1022334.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(660..1970)
                     /locus_tag="IW715_001729"
     CDS             complement(660..1970)
                     /locus_tag="IW715_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1022335.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW715_001730"
     CDS             complement(2152..3381)
                     /gene="rpsA"
                     /locus_tag="IW715_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1022336.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3516..4196)
                     /locus_tag="IW715_001731"
     CDS             complement(3516..4196)
                     /locus_tag="IW715_001731"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1022337.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4245..4880)
                     /locus_tag="IW715_001732"
     CDS             complement(4245..4880)
                     /locus_tag="IW715_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1022338.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4946..6349)
                     /locus_tag="IW715_001733"
     CDS             complement(4946..6349)
                     /locus_tag="IW715_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1022339.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6336..7367)
                     /locus_tag="IW715_001734"
     CDS             complement(6336..7367)
                     /locus_tag="IW715_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022340.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7398..7619
                     /locus_tag="IW715_001735"
     CDS             7398..7619
                     /locus_tag="IW715_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1022341.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7784..8521)
                     /locus_tag="IW715_001736"
     CDS             complement(7784..8521)
                     /locus_tag="IW715_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1022342.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8674..10014)
                     /locus_tag="IW715_001737"
     CDS             complement(8674..10014)
                     /locus_tag="IW715_001737"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1022343.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10251..11774)
                     /locus_tag="IW715_001738"
     CDS             complement(10251..11774)
                     /locus_tag="IW715_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1022344.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12152..12763)
                     /locus_tag="IW715_001739"
     CDS             complement(12152..12763)
                     /locus_tag="IW715_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1022345.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12886..13011)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13093..13809)
                     /locus_tag="IW715_001740"
     CDS             complement(13093..13809)
                     /locus_tag="IW715_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1022346.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW715_001741"
     CDS             complement(13815..14384)
                     /gene="scpB"
                     /locus_tag="IW715_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1022347.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14368..15165)
                     /locus_tag="IW715_001742"
     CDS             complement(14368..15165)
                     /locus_tag="IW715_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1022348.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15135..15506)
                     /locus_tag="IW715_001743"
     CDS             complement(15135..15506)
                     /locus_tag="IW715_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1022349.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW715_001744"
     CDS             complement(15525..16412)
                     /gene="xerD"
                     /locus_tag="IW715_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1022350.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5508 t
ORIGIN      
        1 taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg gaatctctgt
       61 ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag ccctagagcc
      121 acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac ctggtttgaa
      181 tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt tacgaccttt
      241 gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa ctttttcgcc
      301 gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag ttgcatcttt
      361 tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt ttgccatgga
      421 tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa actttctgag
      481 tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat cacgatatca
      541 tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt agtcatagaa
      601 acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg gtaaaatgcc
      661 tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa tgcttttctg
      721 atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc attgacaaaa
      781 acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt caggcgtttt
      841 cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc attcaacaca
      901 gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc aggcagacgt
      961 tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc aagataccgg
     1021 aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt ctttacaaga
     1081 tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta acccgctact
     1141 cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac gatatcagaa
     1201 cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc gtatactttt
     1261 ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact ttcagaaacg
     1321 aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat tacacgttct
     1381 tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc aatcaaacta
     1441 aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt cactgcttca
     1501 tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg atcgcctagc
     1561 cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac tttattgact
     1621 gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc atctgcgtct
     1681 gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc aatcgcgatt
     1741 tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat tccgcctgtg
     1801 tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg atcacgagtt
     1861 actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt aaaaatcgta
     1921 gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat attatttcct
     1981 cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt caagcataga
     2041 ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt ttcaaaagaa
     2101 aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca attatttttc
     2161 agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta aaatgtctcc
     2221 catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     2281 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     2341 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     2401 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     2461 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     2521 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     2581 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     2641 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     2701 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     2761 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     2821 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     2881 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     2941 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     3001 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     3061 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     3121 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     3181 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     3241 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     3301 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     3361 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     3421 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
     3481 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
     3541 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
     3601 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
     3661 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
     3721 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
     3781 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
     3841 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
     3901 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
     3961 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
     4021 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
     4081 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
     4141 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
     4201 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
     4261 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
     4321 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
     4381 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
     4441 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
     4501 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
     4561 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
     4621 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
     4681 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
     4741 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
     4801 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
     4861 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
     4921 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
     4981 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
     5041 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
     5101 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
     5161 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
     5221 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
     5281 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
     5341 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
     5401 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
     5461 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
     5521 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
     5581 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
     5641 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
     5701 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
     5761 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
     5821 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
     5881 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
     5941 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
     6001 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
     6061 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
     6121 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
     6181 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
     6241 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
     6301 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
     6361 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
     6421 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
     6481 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
     6541 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
     6601 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
     6661 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
     6721 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
     6781 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
     6841 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatc
     6901 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
     6961 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
     7021 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
     7081 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
     7141 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
     7201 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
     7261 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
     7321 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
     7381 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
     7441 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
     7501 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
     7561 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
     7621 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
     7681 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
     7741 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
     7801 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
     7861 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
     7921 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
     7981 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
     8041 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
     8101 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
     8161 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
     8221 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
     8281 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
     8341 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
     8401 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
     8461 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
     8521 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
     8581 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
     8641 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
     8701 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
     8761 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
     8821 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
     8881 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
     8941 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
     9001 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
     9061 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
     9121 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
     9181 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
     9241 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
     9301 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
     9361 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
     9421 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
     9481 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
     9541 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
     9601 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
     9661 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
     9721 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
     9781 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
     9841 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
     9901 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
     9961 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    10021 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    10081 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    10141 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    10201 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    10261 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    10321 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    10381 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    10441 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    10501 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    10561 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    10621 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    10681 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    10741 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    10801 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    10861 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    10921 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    10981 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    11041 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    11101 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    11161 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    11221 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    11281 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    11341 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    11401 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    11461 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    11521 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    11581 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    11641 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    11701 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    11761 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    11821 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    11881 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    11941 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    12001 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    12061 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    12121 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    12181 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    12241 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    12301 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    12361 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    12421 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    12481 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    12541 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    12601 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    12661 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    12721 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    12781 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    12841 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    12901 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    12961 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    13021 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    13081 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    13141 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    13201 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    13261 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    13321 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    13381 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    13441 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    13501 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    13561 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    13621 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    13681 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    13741 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    13801 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    13861 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    13921 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    13981 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    14041 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    14101 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    14161 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    14221 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    14281 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    14341 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    14401 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    14461 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    14521 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    14581 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    14641 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    14701 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    14761 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    14821 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    14881 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    14941 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    15001 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    15061 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    15121 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    15181 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    15241 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    15301 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    15361 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    15421 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    15481 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    15541 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    15601 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    15661 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    15721 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    15781 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    15841 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    15901 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    15961 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    16021 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    16081 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    16141 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    16201 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    16261 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    16321 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    16381 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    16441 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    16501 tagacactca gacggactgg tttctttt
//
LOCUS       DACEMK010000059        16517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEMK010000059 DACEMK010000000
VERSION     DACEMK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.061"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..250)
                     /locus_tag="IW715_001745"
     CDS             complement(53..250)
                     /locus_tag="IW715_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1022351.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(644..2371)
                     /locus_tag="IW715_001746"
     CDS             complement(644..2371)
                     /locus_tag="IW715_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1022352.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2364..3557)
                     /locus_tag="IW715_001747"
     CDS             complement(2364..3557)
                     /locus_tag="IW715_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1022353.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3744..4388)
                     /locus_tag="IW715_001748"
     CDS             complement(3744..4388)
                     /locus_tag="IW715_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1022354.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4482..4616)
                     /locus_tag="IW715_001749"
     CDS             complement(4482..4616)
                     /locus_tag="IW715_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1022355.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4618..6750)
                     /gene="feoB"
                     /locus_tag="IW715_001750"
     CDS             complement(4618..6750)
                     /gene="feoB"
                     /locus_tag="IW715_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1022356.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6747..7220)
                     /locus_tag="IW715_001751"
     CDS             complement(6747..7220)
                     /locus_tag="IW715_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1022357.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7621..7845
                     /gene="nrdH"
                     /locus_tag="IW715_001752"
     CDS             7621..7845
                     /gene="nrdH"
                     /locus_tag="IW715_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1022358.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8004..10163
                     /gene="nrdE"
                     /locus_tag="IW715_001753"
     CDS             8004..10163
                     /gene="nrdE"
                     /locus_tag="IW715_001753"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1022359.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10213..11178
                     /gene="nrdF"
                     /locus_tag="IW715_001754"
     CDS             10213..11178
                     /gene="nrdF"
                     /locus_tag="IW715_001754"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1022360.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11267..12406)
                     /locus_tag="IW715_001755"
     CDS             complement(11267..12406)
                     /locus_tag="IW715_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1022361.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12715..13428)
                     /locus_tag="IW715_001756"
     CDS             complement(12715..13428)
                     /locus_tag="IW715_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1022362.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13682..14383)
                     /gene="nagB"
                     /locus_tag="IW715_001757"
     CDS             complement(13682..14383)
                     /gene="nagB"
                     /locus_tag="IW715_001757"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1022363.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14617..16149
                     /locus_tag="IW715_001758"
     CDS             14617..16149
                     /locus_tag="IW715_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1022364.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16340..>16517)
                     /locus_tag="IW715_001759"
     CDS             complement(16340..>16517)
                     /locus_tag="IW715_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1022365.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5123 a         3412 c         2864 g         5118 t
ORIGIN      
        1 agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca ttttagcact
       61 tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata caagtagttg
      121 ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt ccataatatt
      181 ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac aaaattttta
      241 aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt ctaacattca
      301 ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg aataagaatt
      361 taatcacttt taagtatata aactatgtat attctttaat attttttgag aatgcacttc
      421 gtacataata aactttaatc acattagtct ttcataccta cgatcgatct ctttgactcg
      481 cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat gagctacgca
      541 ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa atttggatat
      601 ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt ttccagcttt
      661 cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga tcttttccat
      721 tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa ttgtctaata
      781 ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta tggatctgct
      841 cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat ttggcaaaca
      901 tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc gcttgaccta
      961 gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc aagttgtcat
     1021 atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg attcctatga
     1081 ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg taggctggca
     1141 ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact tgatctttat
     1201 attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg gcatctgact
     1261 cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc gttacatcat
     1321 agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct gtatcccatt
     1381 caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc cctataccaa
     1441 tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt tttttacttt
     1501 tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac
     1561 ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca ttcccgactt
     1621 gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg atcatagagg
     1681 caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc atgatcgtac
     1741 ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc ccgaatgaat
     1801 cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta aaacgaacgg
     1861 ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg ataccgaaaa
     1921 atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag tcaaggatcg
     1981 gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat ggtaccccaa
     2041 tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc aaatcattcc
     2101 atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta agtcctggac
     2161 gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa ggagctggaa
     2221 tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg
     2281 aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc cagtttgcca
     2341 ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt ttctgctaat
     2401 gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc tacaactgca
     2461 agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat gtctttccca
     2521 atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt cctagcttct
     2581 aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac agccatcagc
     2641 atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc agattcgata
     2701 ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga acgatcgact
     2761 tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc ttcacctgtt
     2821 ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat ccgtaaagct
     2881 tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg caggtccagt
     2941 aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc
     3001 agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg ttgcatccca
     3061 ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt ttctaatgct
     3121 ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa cccgtattct
     3181 gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac catattgatt
     3241 ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc ttctccatca
     3301 atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat caatgtggat
     3361 ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac gtcaaaattc
     3421 acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt cggctggtga
     3481 tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt taaatgtttt
     3541 acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt ctttctgaaa
     3601 aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa agaaatttgc
     3661 ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta gccaagttaa
     3721 tattttttta atatttcaca caatcattct gcaagctgat gctccgggta aaaaaagttt
     3781 gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat
     3841 ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc gataactgtg
     3901 gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg cattgctttt
     3961 tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg cttcgtttga
     4021 ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg cttctccaca
     4081 cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata ttgcaatagg
     4141 aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg tacttccatg
     4201 attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga agcattgtac
     4261 gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg gttttcaacg
     4321 atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat ttttttcttt
     4381 ccagacattt tctttcctct ttcttttttt actttccctc atcttactat gctctagcaa
     4441 aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt gatctgttcc
     4501 ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt taaacgagta
     4561 tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt cgccatccta
     4621 ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg gctgacggaa
     4681 caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta acgctacctg
     4741 tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat aagctaaacc
     4801 gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc gatggatagc
     4861 cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg aataagctgc
     4921 tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt cggatacttc
     4981 cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga ccaatcctgt
     5041 aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa caactgccag
     5101 gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag cataaaaatt
     5161 ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc ctgctttttt
     5221 aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt gaggaaaatg
     5281 atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac gtgttttttt
     5341 caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag aaggagctac
     5401 ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca actttgcaga
     5461 acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt
     5521 gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag gaatgaagga
     5581 ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga tcctcgccat
     5641 atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt gcggtagaaa
     5701 tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct gcatccatcc
     5761 cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg taccaaacaa
     5821 tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca gataatagat
     5881 cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg caatctgatc
     5941 gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga gtgtacatag
     6001 ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt cgtccaatag
     6061 tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg ctatctcgta
     6121 atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc gtttcttgcc
     6181 gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac gtggatcata
     6241 tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct tttcgattag
     6301 tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac caagaccata
     6361 ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca tcatattcag
     6421 accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa gatttcgttc
     6481 taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa ccaaataatc
     6541 tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg gtaggtcctg
     6601 aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg ttacgcccgg
     6661 ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag ttttcccgct
     6721 gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat caagattagt
     6781 tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc gattggatct
     6841 cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc catatccatt
     6901 aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt ctctcctgct
     6961 tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt gacaaagatt
     7021 tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa aatggcattg
     7081 ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat attttgcaaa
     7141 tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa attctgcttt
     7201 gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg tattaaaatc
     7261 attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat cattctcaca
     7321 tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga gcgaatcttt
     7381 agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac aattttttct
     7441 ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt caaaaacaca
     7501 aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt
     7561 actaagagtt ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc
     7621 atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt
     7681 ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta
     7741 gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca
     7801 gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat
     7861 caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc
     7921 caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata
     7981 cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca
     8041 agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag
     8101 aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttccatcta
     8161 ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat
     8221 tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt
     8281 tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg
     8341 gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga
     8401 acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc
     8461 cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt
     8521 tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc
     8581 aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg
     8641 ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg
     8701 aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt
     8761 tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg
     8821 aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat
     8881 tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg
     8941 gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa
     9001 acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac
     9061 aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg
     9121 atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg
     9181 tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt aacttaggct
     9241 cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga
     9301 ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa
     9361 acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg
     9421 caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca
     9481 tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag
     9541 ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa
     9601 ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc
     9661 caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc
     9721 aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa
     9781 gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt
     9841 attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata
     9901 tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc gatcaaggca
     9961 tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa
    10021 caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa
    10081 tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc
    10141 aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa
    10201 aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat
    10261 atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc
    10321 ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta
    10381 ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat
    10441 cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc
    10501 atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa
    10561 gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag
    10621 cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt
    10681 ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac
    10741 aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga
    10801 acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa
    10861 acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat
    10921 acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat
    10981 aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat
    11041 gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc
    11101 tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat
    11161 ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag
    11221 tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt
    11281 cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat
    11341 agattttccg gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca
    11401 gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag
    11461 gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt
    11521 ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga
    11581 caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc
    11641 cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc
    11701 tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt
    11761 cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag
    11821 acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg
    11881 taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata
    11941 aactgactaa tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt
    12001 cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg
    12061 tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag
    12121 gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa
    12181 aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat
    12241 aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt ttcaatattg
    12301 gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct
    12361 ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag
    12421 gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg
    12481 cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa
    12541 cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt
    12601 caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta
    12661 tttggctggg cttattactt gatgtaggac atctatttta caacctcaga tcatttattg
    12721 cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac
    12781 atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta
    12841 gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa
    12901 ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt
    12961 agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa
    13021 actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc
    13081 ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc
    13141 cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg
    13201 aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc
    13261 aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc
    13321 tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat
    13381 ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc
    13441 cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac
    13501 gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa
    13561 tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg
    13621 tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa
    13681 attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca
    13741 atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag
    13801 ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc
    13861 ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat
    13921 tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt
    13981 cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg
    14041 catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg
    14101 gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt
    14161 agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt
    14221 cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg
    14281 ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt
    14341 tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat
    14401 attttggtat agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc
    14461 aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga
    14521 tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct
    14581 ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac
    14641 attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct
    14701 ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc
    14761 atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa
    14821 ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa
    14881 actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat
    14941 agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac
    15001 caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc
    15061 gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg
    15121 attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat
    15181 cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg
    15241 tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac
    15301 atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag
    15361 gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag
    15421 accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga
    15481 caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc
    15541 tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc
    15601 atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat
    15661 attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt
    15721 gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt
    15781 tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa
    15841 tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat
    15901 caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt
    15961 gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc
    16021 gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc
    16081 atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa
    16141 ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac
    16201 aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa
    16261 cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct
    16321 tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa
    16381 ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc
    16441 agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt
    16501 acataaacat aaataca
//
LOCUS       DACEMK010000060        15798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEMK010000060 DACEMK010000000
VERSION     DACEMK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.062"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(240..440)
                     /locus_tag="IW715_001760"
     CDS             complement(240..440)
                     /locus_tag="IW715_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1022366.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(745..1977)
                     /locus_tag="IW715_001761"
     CDS             complement(745..1977)
                     /locus_tag="IW715_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1022367.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2234..2803
                     /locus_tag="IW715_001762"
     CDS             2234..2803
                     /locus_tag="IW715_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1022368.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2981..3421)
                     /locus_tag="IW715_001763"
     CDS             complement(2981..3421)
                     /locus_tag="IW715_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1022369.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3579..4343
                     /gene="map"
                     /locus_tag="IW715_001764"
     CDS             3579..4343
                     /gene="map"
                     /locus_tag="IW715_001764"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1022370.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4375..5298
                     /locus_tag="IW715_001765"
     CDS             4375..5298
                     /locus_tag="IW715_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1022371.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5374..6513
                     /locus_tag="IW715_001766"
     CDS             5374..6513
                     /locus_tag="IW715_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1022372.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6506..7306
                     /locus_tag="IW715_001767"
     CDS             6506..7306
                     /locus_tag="IW715_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1022373.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7306..8133
                     /locus_tag="IW715_001768"
     CDS             7306..8133
                     /locus_tag="IW715_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1022374.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8111..8845
                     /locus_tag="IW715_001769"
     CDS             8111..8845
                     /locus_tag="IW715_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1022375.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8945..9811
                     /gene="rfbA"
                     /locus_tag="IW715_001770"
     CDS             8945..9811
                     /gene="rfbA"
                     /locus_tag="IW715_001770"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1022376.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9825..10397
                     /gene="rfbC"
                     /locus_tag="IW715_001771"
     CDS             9825..10397
                     /gene="rfbC"
                     /locus_tag="IW715_001771"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1022377.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10419..11447
                     /gene="rfbB"
                     /locus_tag="IW715_001772"
     CDS             10419..11447
                     /gene="rfbB"
                     /locus_tag="IW715_001772"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1022378.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11545..12396
                     /gene="rfbD"
                     /locus_tag="IW715_001773"
     CDS             11545..12396
                     /gene="rfbD"
                     /locus_tag="IW715_001773"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1022379.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12430..14463
                     /locus_tag="IW715_001774"
     CDS             12430..14463
                     /locus_tag="IW715_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022380.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14507..15787
                     /locus_tag="IW715_001775"
     CDS             14507..15787
                     /locus_tag="IW715_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1022381.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         4851 a         2725 c         3281 g         4941 t
ORIGIN      
        1 cctcaaaaag agtatacgaa cgaatgttct ttttgtgaag gcggaattta atatactgaa
       61 taatagaaaa caagtacttg tgccaaatta tgtgccaaaa aaattcgaat tcaacaaaat
      121 gtaaacaaat agaaaacctt ctatattaag atttcctgca ataacaaaca caacagaaga
      181 cgtgtaatag ttagtcagga acgtacaaat aacccctgta tcctttgcgg tacagggggt
      241 tattttgtat aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg
      301 ctaagtatat ttactccttg ataaagtaaa atagatgcat gcgtatgtct tagtccctca
      361 ataaacagtc gaagagaaaa taggcaaaaa ttgattacta aagaaaaact tactaggtcg
      421 acggtgtagt tgattttcat acttcaacag attcgaatta aacgatttcg attggcttat
      481 accagtccta tccgacgaaa gatatatcgt tcttaactct agcggtataa cttgaagctt
      541 tacgtaaagc caatgggaat gaactggaga tgacttttta ttatttttag taatagctga
      601 tgggttaata cttacaactt tccttttcaa agtaccatat aaaaaaacgg tggggttgtt
      661 tagcgaaata aaggatctaa gccaatagct tagatccttt attctatgct ttatattatt
      721 ttttgcatgt gtgcgaaagt atactcaagc ccaatcgcca tttcggaaaa tcggtactgt
      781 agagccatct tcacggattc cgtcgatatc catcttgtca gatcctacca tgaaatctac
      841 gtgggtttga cttctattca atccagcttc tgctaattct tcatcggaca tttctgtacc
      901 gcctttcaca ctgaaggcat aagcagaacc tagtgctaag tgattcgatg cgttttcatc
      961 aaataatgta ttgaaaaaga tgatgccaga ttgagaaata ggtgaagggt caggtacaag
     1021 tgcgacttct cctaaacgac gcgcaccttc atctgtatca agcagttttg ctaagacatc
     1081 ttctccttgt tcggcagaaa aatcaatgac ttttccatct ttaaaagtaa atttcatacc
     1141 cgagatgatg gtgccagcat aactcagtgg ctttgtactt gaaatgtaac catctactcg
     1201 atgactgtca ggtgctgtga acacttcttc cgtcggcata tttgccatga atttttctcc
     1261 acgagcatta tagcttccag cgccttccca aagatggttt ttaggcaatc cgataatgat
     1321 atctgtaccg ggagcagtat agtgcagggc agaaaattgt tctcgattca attcttctgc
     1381 tttttttgct aatttttcat catgcttctt ccatgctaag acagggtctt cttcatagac
     1441 acgagtcgtt ttgaaaatct gatcccataa tgcctcaact tgtttttctt ctggcagttc
     1501 tgggaagact tttgctgccc attgtttacc agccgcagcc acgacagtcc aactgacttt
     1561 atttgcttga gttgccttgc gtaaattcat tagtgctttg ccagccgctg attgataaga
     1621 cgctactcga tggctatcta caccagcaaa tgcatcggga tcggcagaaa caacactgat
     1681 cctgcttgct cctttttcaa gccattcatc cgcttgatcg attttagatt gtggtacatt
     1741 ctcaatacga tccgttgcag catgtaaaag aaattctctt tggatctggt cgtcagtcca
     1801 ttgaacgata acctcagcgg ctcctagttt ataagcttct tgtgtgatca atcgagcaag
     1861 gggtgcctgt tccacgctga tctgtaaaac aactgtgtgt cctttttctg tggctacgcc
     1921 agtttctgca attaagcgtg catatttttt tagaagttca ttaaaatcag ataacataat
     1981 aaaaattcct cctcaccatt ttttctggta actattagca tgataccatt taattatgat
     2041 tcaaacaatg aggcctttaa ataaaaaaaa gaggttattt ttttaaggtt aagtttcatg
     2101 atatatgtta ctacttgcta tttcagcttg tgaagaaaat caaaaactaa ttttgcatat
     2161 caaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa aaaattaagg
     2221 agattaaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     2281 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     2341 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     2401 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     2461 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     2521 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     2581 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     2641 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     2701 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatac aagacagtat
     2761 agatgctatc ttgaaattca aagaaccaat agaaatcgat tgaatatact caaaagttcc
     2821 acaaatcagt agataacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2881 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccgtac
     2941 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     3001 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     3061 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     3121 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttcccg
     3181 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     3241 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     3301 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     3361 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     3421 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     3481 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     3541 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     3601 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     3661 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3721 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3781 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3841 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3901 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3961 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     4021 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     4081 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     4141 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     4201 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     4261 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     4321 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     4381 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     4441 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     4501 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     4561 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     4621 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     4681 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4741 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4801 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4861 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4921 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4981 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     5041 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     5101 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     5161 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     5221 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     5281 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttataa
     5341 taattatgac tcttaattaa agtaggtgcc catatgcttg tttcttttag tatggttata
     5401 agattttttc tgacaatttt attatcactg atattaacac ctgtattcaa aatcatttca
     5461 gtacaaacag gtatggtcga caaaccaaat gaaagaagaa taaataaagt tccaatgcct
     5521 tcagcgggcg gactgcctat ctttatttca tttgttatat cgactttatt cttattccgt
     5581 aaaattatcc ctcaatcgta tattgtaccg atcttgattg cttctttagt aatcatagtc
     5641 acaggagtgc tcgatgataa atatgagctg acacctaaac aaaaaagtat agggatcttg
     5701 attgcttctc ttattattta ttttgtagca gatatacgta ttgattcctt cacgcttcct
     5761 tttattggat atatccaact aggctggctt agttttcccg taacgatttt ttggatcttc
     5821 ggtatcacga atgcggtcaa tctgattgac ggtttggatg ggctggcggc agggatttca
     5881 ttgatcggtt taattacgat aggaatcatt ggatattttt tcctccatgc ttctacagtg
     5941 tatattccag ttgttatttt ctgtcttgta gcaagtatta tcggtttttt tccttacaat
     6001 ttttatccgg caaagattta tttaggcgat acaggagctc tattcctagg gtttatgatg
     6061 gccgtccttt cactacaagg gctgaagaac gtgacttttg tttcatcgat ttctttgctg
     6121 atcgtcatgg gagtgccggt aacagacacg ttttttgcta tcattaggag aaaagcaaac
     6181 cgtgtttctt tttctacagc agataaaaaa catcttcatc accgactaat cagtttaggc
     6241 tttactcata aaggtgctgt cctgacgatt tatgcgatgg ctttgatgtt ttctttcaca
     6301 gctatggtca tgaattatac tggaagaatc ggaacgatca tattgattat tgccatgttg
     6361 tttgctgcaa ttttattatt tgaattgatt ggtctgatca atgagaaaca tcagtttatt
     6421 ttaaaaacat tgcgattttt agggaataaa gagtaccgta cgcaagtaaa acagaaatat
     6481 ttgaagaaat ggaaccgtca tatagatgaa taacaaatta aaccagccga tccttattag
     6541 tattgtgacg gcaaacagtg ataaaatttt tcggacacta gataatttta tttcaactgt
     6601 aaaagatgaa acagcagtaa aaatccgaat ttttgacaac aattcacttc ccgagtacaa
     6661 aaaaagacta acagagtata ggaaatatcc ctacatcgat atctattttc atgatgaaaa
     6721 ttccggattt ggttatggac ataatcacaa tctgatagct agcgattctc gctatggcat
     6781 tatttgtaac ccagatattt tagtggatga agagacagtt acagcattgg tcttgttatt
     6841 gaaagaacat ccggaatgtg cgatgttagc acctaagatt cttaatgaag atggaacaac
     6901 tcagcacctc atccgtgagc agctggctgt ttttgattac gttttgcgct ttgtgccttt
     6961 caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg tttgaatgcc gaggattgcc
     7021 tactgatgct aatagttatg tacggatgat ctcaggtagt tttatggtag ctgatattga
     7081 taaatttaaa gagataaagg ggtttgatga gcgtttcttc atgtactttg aagacaacga
     7141 cttgtgtttg agttttgaaa aagcaggtta caagttattg tacacaccct tttatgcagt
     7201 tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa ctgtttcgcg tgtttttgac
     7261 ttctatgatg aaatttttca ataagtgggg gtggcgtttt ttctgatgaa accagaaata
     7321 ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat ctccagctta tggaccatta
     7381 agcaaagcac ttagcgaaaa gcaaattcag cttattattt acgataatag tcctcaaagg
     7441 cagcaaaatg agctgtttga aaaagaaaat atcgtttatc atcatgatcc gcaaaatcct
     7501 ggtttagcgg tggcttataa ttttgcgttg tctcaagcag atgaaggaac acgttgtctc
     7561 gtcactctgg atcaggatac gaggctagtc gacgattatt ttgaactatt gcgtaaagta
     7621 acctttacag atgaatgtgt tgctgcagtt ccgatgattt tttctgggag ccgacaagtt
     7681 tctcccctat attcagatcg ttatatcaat cgtacagctc aagcagtaga agttgaaggc
     7741 ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg cctggtctgt caaatttcta
     7801 aaaaagattg gcgggtttaa tcctgctttt tctttagatt ttttggatca ttggctgttt
     7861 tggaaagtgg atcagttgga aaaaactgtg gagatccttc ctgtacgaat ggaacatgat
     7921 ctttcggttt tagactatga aaaagtttca agtaatcgat accgttctat tttacaagca
     7981 gaaaatcgat tttatcatga atatgatcaa aggcatttat ccagtcaccg acgacaatta
     8041 ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc ggcatatctg gcggatgact
     8101 ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg cgttcggttt gtatagctac
     8161 ctttaatgga ggcagatatt taagagagca gttagatagt attttatctc agctttcagc
     8221 agaagatgaa gtagtcattt ctgatgatgg gtcagtagat gatacatggg atatattaac
     8281 tgaatacgca gaaaaagata agcgaatcca gcttttttcg gggcctcgtc aaggattgat
     8341 cgctaatttt ggttatgcga tccagcagac gaaaggagat ctaatctttt tggcagacca
     8401 agatgatgtt tggttggaga caaaagtgaa aaagatcact gactattttg agcagaatcc
     8461 ggatcagcta gtagtagttt ctgatttaat cattgtggac agcgagctgc aagagatcca
     8521 tgattcgtat ttttcgtttc gaaattccaa agaaggatgg cttaataatg tgataagaag
     8581 tcactatatt ggtgctggta tggctttccg gtcttcttta aaacccgata ttttgccgat
     8641 cccgtcagaa gtgccgatgc atgatatgtg gattggtttg ctgggaggaa agaagactgg
     8701 gttcctaaga gaaccgttaa ctctgtaccg aagacatgat ttaaacgcga gtgagattga
     8761 aacacattct aaattacggc aaaaaatttc ttggcgtgtt catctgatta cggcgttgat
     8821 caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa tccttaatat ttccatgaca
     8881 aatataacaa ttgatgagat aataacaaag tgaaatttga aatcaaaaag gagagcagat
     8941 atccatgaag ggaatcattc ttgctggggg cagcggaaca cgtctgtacc ctttaacaaa
     9001 ggcaacatcg aaacaattga tgccgattta tgacaaacca atgatttatt atccaatgtc
     9061 tactctgatg ttggcgggaa tcaaagaaat tctgattatt tcaacgccac aagatacacc
     9121 acgtttcaag gaattatttg gaaacggaaa tgatttagga ctgcagatcg aatatgcagt
     9181 acaagaaagt ccagatggat tggcacaagc gtttatcatt ggagaagaat ttattggaga
     9241 tgatagtgtc tgcttggtat taggggacaa tatttactat ggtggcggtc tgtctaaaat
     9301 gttgcaacga gcagcttcaa aagatacagg cgcgacagta tttggctatc atgtcaatga
     9361 tccagaacgc tttggtgtag tagaatttga tgagaatatg caagctttgt cgattgaaga
     9421 aaagcctgct cagccaaaat caaattacgc tgtaactggc ctttattttt atgacaatga
     9481 ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt ggagaactag agattacgga
     9541 tgtcaataaa gcatatttgg ataaaggaaa actatccgta gaacttatgg gacgcggatt
     9601 tgcttggctt gatacaggaa cacatgaatc attgcttgaa gcatctacct ttatcgaaac
     9661 aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa gaaattgctt accgtatggg
     9721 ctacatcgat aaggaacaat tgctggccct tgcacagcca ttgaaaaaaa atcaatacgg
     9781 tcaatattta ttgaatttgg ccgctgaata aggagtcctt acagatgaaa gtaacagata
     9841 caaaattaac agatgtcaaa atcgtagaaa tggacgtctt tggcgatcat cgcggtttct
     9901 tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg attgaaatat gactttgttc
     9961 aagacaacca ttcactatca gctgaagctg gtgtccttcg ggggcttcat ttccaaaaag
    10021 gagaagctgc acagacaaaa ttgattcggg tagtcactgg agcagtttta gatgtagtag
    10081 tggatatccg caaaggtagc ccgacttatg gtcaatggga cggatttatc ttatctgaac
    10141 ataatcatcg acagttgctt gtacctaaag gatatgccca tggattcgtt actttgacac
    10201 caaatgtgaa cttcctttat aagtgtgata attactataa tgcagaagca gatgggggca
    10261 tcgcatttga tgatccagat ttagcgattg attggccaat tgatccatca agagcgatta
    10321 tgtcggaaaa agatcaccgt catccgacat taaaagaatt tgaagcagaa aatccatttg
    10381 tttacggaga aatctaagaa ttaaggagaa attttcttat gaaaaacatc attgttaccg
    10441 gaggtgccgg attcatcggc tctaatttcg ttcattacgt ggtaaacaat catccagaag
    10501 tgcatgtcac tgttttggat aaactgactt atgcaggaaa caaagagaac ttggctggac
    10561 ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc agatgcagaa ttagtggacc
    10621 gtctagtaaa agaagcggac gcagttgttc attacgcggc agaatcccat aacgataatt
    10681 cattaaaaga tcctttccca tttgtacaaa cgaatatcat tggtacgtat acacttttag
    10741 aagcttgccg caagtatgat gtacgctatc atcacgtatc tactgacgag gtttacggcg
    10801 atcttccatt acgtgaagac ctgccaggac atggggaagg cgaaggagaa aaattcactg
    10861 cggaaacacc atataatcct tcaagtccat attcttctac aaaagctggt tcagatctgt
    10921 tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac catttcaaac tgttcaaaca
    10981 actatggtcc ttatcagcat attgaaaaat ttattcctcg ccaagtaacg aatatcttaa
    11041 gcggtattcg tccaaaatta tatggcgaag gaaagaatgt tcgtgactgg atccatacga
    11101 atgatcattc ttcagcagtg tggctgattt tgacgaaagg cagaattggc gaaacctatc
    11161 tgatcggtgc agacggtgaa gaagataata agaccgtgat ggaattgatc ttagaaatga
    11221 tgggtcaacc aaaagatgcg tacgaccatg taaacgaccg tgcaggacac gacttgcgtt
    11281 atgcaatcga ttcgacaaaa ttacgggaag agttaggatg gaagcctgag tttacgaact
    11341 tccgtgatgg gttggctgac acgatcaaat ggtacgaaga acatgaagac tggtggaaaa
    11401 aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg acaataatac agagaaaatg
    11461 tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc gcctgtctct tttttaaaat
    11521 aaaaatcaag gagcggataa tcatatgttt ttattaactg gaggaaatgg gcaactaggt
    11581 acagaattgc gccatttatt agatgaaaaa aatctgcaat atgtttcaac agatgcaaaa
    11641 gacttggata tcacagatgc cgagaaaaca atggcttaca tcacagaatt aaaacctgat
    11701 gttattttcc attgtgcagc ttacacagca gtagataaag cagaagaaga agcaaaagaa
    11761 ttagatgaga agatcaatgt tgatggtaca agaaatgtag cacttgcagc aaaagctgtg
    11821 aatgctcaat tcgtctatat cagtacagac tatgttttcg atggaaaaaa caaaaatgta
    11881 gaatacgaag tagacgacca aacgaatcca ctaaatgaat atggtcgtac gaaattatta
    11941 ggcgaaaaag cagtagaaga gattttagaa gactattata tcatacgaac ttcttgggtt
    12001 ttcgggatct atggacacaa tttcgtcttc acgatgcaac gcctagcgga aacccgcgac
    12061 aaactgactg tagtaaatga ccagtttggg cgaccaactt ggacacgtac tttggcagaa
    12121 tttatggtat atgtcatcga agaaaaagca ccatttggta tttatcattt gtcgaacgaa
    12181 aatagctgtt cttggtatga attcgcaaaa gaaatattga aagatacaga tgtagaagtt
    12241 gcacctgtta cgtcagaaga atttccgcaa aaagcaacac gtccgcagta ttcagtcatg
    12301 agcctgaaaa agacagaaga gttaggattt gtcataccaa cttggcaaga agcacttgcg
    12361 caaatgctgg aagctgccaa aaaaatgaat aagtaatttc cttttatcaa atagaatttg
    12421 gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc aaaagcgaac
    12481 caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct tattggcgtt
    12541 ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg tgtttgggat
    12601 aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg acagaatcgg
    12661 gaggtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca agctgaagag
    12721 gactttccgt tggtgaacga ggattattca ttaaacggac aaaacatgat cattgcgtat
    12781 aattctcctg caaaggatat tacggttatc ggaaaaccat tcaactgggg attctttttc
    12841 ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt catggtcttg
    12901 cttggattcg aagtactgat gatattaacg aagagaaaaa aagctctatc gctaatcggt
    12961 gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca tgtgggcgat
    13021 ttgattttct ttactttggg actgatgact gctttttatc attatttcta caatcatgat
    13081 aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat tgggtttatt
    13141 ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt ttattttgtt
    13201 ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt agtgttgatt
    13261 ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac gtcaaaagac
    13321 gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac tggaggaaag
    13381 tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt tgaagatatc
    13441 aactattcga ataactctga agtggccctg ttctatcact tttttccaac tgtattccta
    13501 gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact gggacgtatt
    13561 ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc aaaatggcta
    13621 gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac ttttagtttt
    13681 gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga tgttattcct
    13741 atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta tgcgattacg
    13801 cattcgaaaa tagataatta tttttcggat tttgaaacca tcgcagttgc tgtgttggga
    13861 acagtgattt tagcatttgc attatttggc tggagacgct tgttttacct cgcttttgcc
    13921 gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac aggagcgatt
    13981 tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc tggtaaagtt
    14041 tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa atccttcaat
    14101 acagtacgct tttatccaga tatagctgca tggaaggaaa tcgatccgaa agaagaatac
    14161 gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga agatacaaag
    14221 ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga cgcagaaaaa
    14281 ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca attatatgaa
    14341 gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt taaagtagaa
    14401 tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct agtcgtacag
    14461 taataggcta gctgagaata atcctgaaat attagtagaa aggagagtgg agataattca
    14521 agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt tgttgctgac
    14581 gctagtcttt tttgtttgga cagcagggtt aggttttcca gcaactttgt ttatatataa
    14641 caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa atgtttataa
    14701 agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca ttttcttttc
    14761 ttactttcgg gaattaagaa atgagtcttt atttggagat ttactgtttc ctttgattat
    14821 tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg gtatttatct
    14881 gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag agatccaagt
    14941 accagaagga caaagcatct gggctccagg caattcgctt tctgatattt ggatcaatac
    15001 gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat ttgccagttc
    15061 ttttgaacga tggtatgtac gaatcatcgg tgcagtagca gttgttgcag cttttctcgg
    15121 gatttgggtc tgccaatcta gaggtgcatt agtcgcgttg attgtttttg cgatattgga
    15181 tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgctt acacggttac
    15241 gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag tcaatttgtt
    15301 taccggtcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa aatctgaaca
    15361 agttttacta ggaatgaaaa catttatctt ccaacgagga aaccaatttt taggaaacca
    15421 caatagttat aattctattt tgaatatcta tggattgata ggatttggga ttgctacttt
    15481 gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca acgggcagat
    15541 gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg atacattgac
    15601 ttcataccca tgggttccga ttatttatat cctgttggct atggccgttc atcgatatga
    15661 tgaaccagaa gttgcggagg aattctatgt tatggaagag gcatatgacg atatgactta
    15721 tgatgataca tcttatgggg aacaaccaga tagcgctcat tcttccagga tccaacgcta
    15781 tcattgatag gcacaaaa
//
LOCUS       DACEMK010000061        15730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEMK010000061 DACEMK010000000
VERSION     DACEMK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.063"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(52..522)
                     /locus_tag="IW715_001776"
     CDS             complement(52..522)
                     /locus_tag="IW715_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1022382.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IW715_001777"
     CDS             complement(681..1139)
                     /locus_tag="IW715_001777"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1022383.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IW715_001778"
     CDS             complement(1310..2455)
                     /locus_tag="IW715_001778"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1022384.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IW715_001779"
     CDS             complement(2468..3385)
                     /locus_tag="IW715_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1022385.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IW715_001780"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IW715_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1022386.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="IW715_001781"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="IW715_001781"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1022387.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="IW715_001782"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="IW715_001782"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1022388.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="IW715_001783"
     CDS             6911..7792
                     /locus_tag="IW715_001783"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1022389.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="IW715_001784"
     CDS             7789..8505
                     /locus_tag="IW715_001784"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1022390.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="IW715_001785"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="IW715_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1022391.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="IW715_001786"
     CDS             9222..10223
                     /locus_tag="IW715_001786"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1022392.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="IW715_001787"
     CDS             10220..11581
                     /locus_tag="IW715_001787"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1022393.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="IW715_001788"
     CDS             11541..12692
                     /locus_tag="IW715_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1022394.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="IW715_001789"
     CDS             12667..13836
                     /locus_tag="IW715_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1022395.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="IW715_001790"
     CDS             14011..15390
                     /locus_tag="IW715_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1022396.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5140 a         2771 c         3346 g         4473 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aaccattagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatttg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa
//
LOCUS       DACEMK010000062        15351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEMK010000062 DACEMK010000000
VERSION     DACEMK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.064"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..563)
                     /locus_tag="IW715_001791"
     CDS             complement(<1..563)
                     /locus_tag="IW715_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1022397.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IW715_001792"
     CDS             complement(750..1400)
                     /locus_tag="IW715_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022398.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW715_001793"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IW715_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1022399.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IW715_001794"
     CDS             complement(2912..3910)
                     /locus_tag="IW715_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1022400.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW715_001795"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IW715_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1022401.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IW715_001796"
     CDS             complement(4926..5657)
                     /locus_tag="IW715_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1022402.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IW715_001797"
     CDS             complement(5661..6926)
                     /locus_tag="IW715_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1022403.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW715_001798"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IW715_001798"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1022404.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW715_001799"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IW715_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1022405.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IW715_001800"
     CDS             12268..13770
                     /locus_tag="IW715_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1022406.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IW715_001801"
     CDS             13772..14509
                     /locus_tag="IW715_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022407.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="IW715_001802"
     CDS             complement(14554..14727)
                     /locus_tag="IW715_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1022408.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..>15351
                     /locus_tag="IW715_001803"
     CDS             14703..>15351
                     /locus_tag="IW715_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1022409.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAF"
BASE COUNT         4519 a         3288 c         2590 g         4954 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg aattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt t
//
LOCUS       DACEMK010000063        15241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEMK010000063 DACEMK010000000
VERSION     DACEMK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.065"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(58..360)
                     /locus_tag="IW715_001804"
     CDS             complement(58..360)
                     /locus_tag="IW715_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022410.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IW715_001805"
     tmRNA           complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IW715_001805"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1117..1890)
                     /locus_tag="IW715_001806"
     CDS             complement(1117..1890)
                     /locus_tag="IW715_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1022411.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2034..2384
                     /locus_tag="IW715_001807"
     CDS             2034..2384
                     /locus_tag="IW715_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1022412.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2365..3081
                     /locus_tag="IW715_001808"
     CDS             2365..3081
                     /locus_tag="IW715_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1022413.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3081..4529
                     /locus_tag="IW715_001809"
     CDS             3081..4529
                     /locus_tag="IW715_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1022414.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4599..5108)
                     /locus_tag="IW715_001810"
     CDS             complement(4599..5108)
                     /locus_tag="IW715_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022415.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5098..5823)
                     /locus_tag="IW715_001811"
     CDS             complement(5098..5823)
                     /locus_tag="IW715_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022416.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6046..8166)
                     /locus_tag="IW715_001812"
     CDS             complement(6046..8166)
                     /locus_tag="IW715_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1022417.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8396..9574
                     /locus_tag="IW715_001813"
     CDS             8396..9574
                     /locus_tag="IW715_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1022418.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9590..10348
                     /gene="aroD"
                     /locus_tag="IW715_001814"
     CDS             9590..10348
                     /gene="aroD"
                     /locus_tag="IW715_001814"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1022419.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10371..10856
                     /gene="ybaK"
                     /locus_tag="IW715_001815"
     CDS             10371..10856
                     /gene="ybaK"
                     /locus_tag="IW715_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1022420.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10927..12180)
                     /locus_tag="IW715_001816"
     CDS             complement(10927..12180)
                     /locus_tag="IW715_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1022421.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12297..13646
                     /gene="gdhA"
                     /locus_tag="IW715_001817"
     CDS             12297..13646
                     /gene="gdhA"
                     /locus_tag="IW715_001817"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1022422.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13758..15104
                     /locus_tag="IW715_001818"
     CDS             13758..15104
                     /locus_tag="IW715_001818"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1022423.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5017 a         2713 c         3088 g         4423 t
ORIGIN      
        1 cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta
       61 atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc
      121 gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat
      181 tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa
      241 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
      301 gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat
      361 aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat
      421 tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg
      481 ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
      541 taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg
      601 aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg
      661 attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt
      721 ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc
      781 acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt
      841 tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga
      901 aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg
      961 gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa
     1021 agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga
     1081 ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa
     1141 tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa
     1201 tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc
     1261 attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag
     1321 acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc
     1381 attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga
     1441 taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac
     1501 atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat
     1561 tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt
     1621 ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa
     1681 agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc
     1741 tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat
     1801 agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc
     1861 aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt
     1921 ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg
     1981 gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa agaatgattt
     2041 ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa
     2101 aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt
     2161 ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat
     2221 tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc
     2281 tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg
     2341 acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt
     2401 tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat
     2461 ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta
     2521 gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca
     2581 agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc
     2641 ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct
     2701 cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt
     2761 tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa
     2821 gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt
     2881 actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg
     2941 gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa
     3001 aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc
     3061 gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa
     3121 tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga
     3181 ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc
     3241 aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc
     3301 tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg
     3361 aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat
     3421 ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac
     3481 aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag
     3541 cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga
     3601 acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg
     3661 cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg
     3721 gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg
     3781 atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa
     3841 atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa
     3901 tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg
     3961 cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg
     4021 attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag
     4081 aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat
     4141 tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa
     4201 tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta
     4261 ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt
     4321 ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa
     4381 atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc
     4441 ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt
     4501 tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga
     4561 tcttctatac cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga
     4621 tcaatataag gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt
     4681 tcttcatgag ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt
     4741 tcatcaaatg tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat
     4801 tgcagcttta ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga
     4861 gagatatcaa gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca
     4921 tccaacagga catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg
     4981 atcgcaaaat tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta
     5041 tgctgttttt cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca
     5101 ggtttcatta cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa
     5161 tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag
     5221 tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat
     5281 cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt
     5341 ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac
     5401 gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat
     5461 aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt
     5521 gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa
     5581 actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt
     5641 agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg
     5701 caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca
     5761 atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt
     5821 catacttttc acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct
     5881 ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga
     5941 agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg
     6001 agcagcgaca ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact
     6061 tgtggtcgtt ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg
     6121 tccgttgtgt tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt
     6181 taatttctct aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact
     6241 attcgtatag aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt
     6301 atcaactttt tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat
     6361 agcttcacca gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac
     6421 aaaatcacca tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc
     6481 taactgaata taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc
     6541 aacttgtccg ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat
     6601 ataagggaca cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc
     6661 aactaattca gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag
     6721 gacgatgact gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac
     6781 ggcggtatct aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt
     6841 ggctaacggg aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac
     6901 agcaatgaat tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg
     6961 gaaaaatggt ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata
     7021 agaagaatca aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa
     7081 tgtaccagcg ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc
     7141 aggtgcagct tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa
     7201 gccaaacaat gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg
     7261 atggaaaaag ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg
     7321 tgtaacttca tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg
     7381 gaagctttct aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc
     7441 atttggtgct gcataatgtt cttttacata attttctact tctttcatat cattgggact
     7501 tgcttctgcc cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc
     7561 attcaagcca agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg
     7621 acgatcagct tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat
     7681 cgcaaaagcc attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag
     7741 cagaacagtc aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg
     7801 aatggcactt tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt
     7861 aatgaagtcc gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag
     7921 aaaataaatg accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat
     7981 tccaagtaat aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc
     8041 actttcgatt ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa
     8101 aacgactagt aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca
     8161 tttcaagata atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta
     8221 cacagctcac ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca
     8281 gaagtttatg ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga
     8341 caagtcctat gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa
     8401 agtaaaagta aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa
     8461 agaagacctt gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca
     8521 aaaccaattt atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt
     8581 gagccagcag aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa
     8641 agaaaaaaga actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg
     8701 agaaggagac gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc
     8761 ttggtataac gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat
     8821 ctacccggaa atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga
     8881 gagccagttc atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt
     8941 aaaatttgcc gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga
     9001 agtacgggga tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag
     9061 ttataccggc gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt
     9121 cgatctagca aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga
     9181 tgtcgatcag caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag
     9241 aaaacaattt ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa
     9301 aaaagtcttt cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt
     9361 agcagaagac gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt
     9421 ccaaacaatg attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt
     9481 atatcgtctg ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa
     9541 agtgtttgtt tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt
     9601 aaagggattg aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt
     9661 ccgagaggaa gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga
     9721 atggcgagtc gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt
     9781 tcttatccat gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga
     9841 aggcggagaa caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca
     9901 cacaggatta gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag
     9961 actgatagag gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa
    10021 agaaacacca gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc
    10081 cgatatcgga aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga
    10141 gttgactaga cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa
    10201 tcttggtaaa atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc
    10261 cttaagcagc gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat
    10321 tattcaatta ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga
    10381 aaaaaatagt aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag
    10441 agcacgaata tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc
    10501 cagaaagcca atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc
    10561 ttgttgctgt gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag
    10621 ggaataaaaa agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac
    10681 gtggtggctg ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg
    10741 cagaaagcta tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag
    10801 cacctcaaga tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca
    10861 tacgtataaa agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt
    10921 tatacattat gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc
    10981 tgtgatatta aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag
    11041 accatcaatg attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc
    11101 gccagttaca aatgatttct caataggaaa agttttgttg attgatagct taaactcttt
    11161 gcatagttga acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc
    11221 catttcaagc cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc
    11281 attgaccaag ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg
    11341 tttggtttgt gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa
    11401 ttctttattg acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt
    11461 gaaaagtaaa tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa
    11521 agtgatgtct tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga
    11581 tatcaagggg gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat
    11641 cttgtaccca tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat
    11701 aatggcagga gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt
    11761 gtggatcaat ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg
    11821 attgagcaat gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg
    11881 atcctcttct tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt
    11941 ccattgcgca aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc
    12001 atcaacagga cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt
    12061 tcctgctttt ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat
    12121 cgctgccatc atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat
    12181 tccctcacct caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg
    12241 agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga
    12301 caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc
    12361 aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca
    12421 ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta
    12481 ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc
    12541 gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg
    12601 tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg
    12661 gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata
    12721 atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat
    12781 cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg
    12841 gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt
    12901 tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac
    12961 atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg
    13021 ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat
    13081 gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag
    13141 aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta
    13201 tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat
    13261 gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca
    13321 aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg
    13381 aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg
    13441 ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc
    13501 agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg
    13561 gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag
    13621 caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt
    13681 ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc
    13741 tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg
    13801 tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac
    13861 gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg
    13921 acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag
    13981 tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct
    14041 tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct
    14101 ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc
    14161 gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt
    14221 tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta
    14281 tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt
    14341 gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg
    14401 gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg
    14461 atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag
    14521 caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac
    14581 caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag
    14641 accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac
    14701 aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc
    14761 gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag
    14821 gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag
    14881 acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt
    14941 atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc
    15001 cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac
    15061 caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc
    15121 tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt
    15181 taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt
    15241 c
//
LOCUS       DACEMK010000064        14994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEMK010000064 DACEMK010000000
VERSION     DACEMK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.066"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW715_001819"
     CDS             complement(84..263)
                     /gene="rpmF"
                     /locus_tag="IW715_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1022424.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(321..890)
                     /locus_tag="IW715_001820"
     CDS             complement(321..890)
                     /locus_tag="IW715_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1022425.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1076..1978
                     /locus_tag="IW715_001821"
     CDS             1076..1978
                     /locus_tag="IW715_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1022426.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW715_001822"
     CDS             complement(2013..3797)
                     /gene="pyk"
                     /locus_tag="IW715_001822"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1022427.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW715_001823"
     CDS             complement(3871..4833)
                     /gene="pfkA"
                     /locus_tag="IW715_001823"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1022428.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW715_001824"
     CDS             complement(5001..8315)
                     /gene="dnaE"
                     /locus_tag="IW715_001824"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1022429.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8546..8731
                     /locus_tag="IW715_001825"
     CDS             8546..8731
                     /locus_tag="IW715_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1022430.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8793..9989)
                     /locus_tag="IW715_001826"
     CDS             complement(8793..9989)
                     /locus_tag="IW715_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1022431.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10159..11595)
                     /locus_tag="IW715_001827"
     CDS             complement(10159..11595)
                     /locus_tag="IW715_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1022432.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11888..12697
                     /locus_tag="IW715_001828"
     CDS             11888..12697
                     /locus_tag="IW715_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1022433.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12883..14880)
                     /locus_tag="IW715_001829"
     CDS             complement(12883..14880)
                     /locus_tag="IW715_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1022434.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4377 a         3178 c         2660 g         4779 t
ORIGIN      
        1 ttttttattt accgactaca aaaaaataaa cctcaaggct ccatggttag ccttgaggtt
       61 ttacattttc taattaaaat gtattaagct tcttttgaca ttacgtcctt accatcatag
      121 taaccacaat ttgcacatac gtgatggctt tttttcattt caccacagtt tggacattcg
      181 tttaaccctt tgattgtcaa tttgtagtgt gtacgacgtt ttgctttttt agcttttgat
      241 gttcttctag ctggtactgc cattttgcta cacctccttg taaagtcttg tatctaaatt
      301 agacacaata tattccaatc ttacttgtta tcgtcgtctt ttgatttaga atcaaaaagt
      361 tcagatagtt ttgcaagacg aggatcaatt ttattctcgg cttcttccat ttttctgcgc
      421 tggtattctt cttcggaaac aacttcccaa tcattgcctt ttggaagttc tgttgctgct
      481 tcctcttctt ttgtcaatac ccgcattgga atcgccaata agatgttgtc ttcaatcgac
      541 tctgctaaat tcaataatgg accttcaatt agcaaaatct catctgcaga catttgttca
      601 tctctacttt ggaattgttc ttctgtcatg aatacttcgt ctacagaaaa atccatgggc
      661 agttcaacag gttctagtga acgtgttgaa ggaactgtca aagtcgtctg cacagtgtaa
      721 tgaacgatat agcctttctt atcaactgag accaaacctt cgacttccac tggcgcaaca
      781 tcaagtatta aattgtctcg cttcatgaga gcggctttca agtcgagcgt ctcatgaaaa
      841 gttaacggtg tctcctggta ttttctcaat tccaataaag accatttcat ttttcatcac
      901 tcctaagcaa caaaagcaat tatacagggc aaaaagcatg ttgtcaatga aattttcttg
      961 acaccttgca gttcataaaa aaacagaaaa atctttatta gaaagcctct gtttgctatc
     1021 atcctcagaa tcatttacaa taatcattat tccattaaag gagcggatga tcttcatgaa
     1081 cacgaaagat atcgaatact atgtccaaat cgtcaaacaa aaaaatttta cgaaagtggc
     1141 taattatttc cgtgtgagcc aacctactat tacatatgca ttacaacgtc ttgaaaaaga
     1201 gtttgacacc gtacttatta caagagatcg atcgcatcac gaattaatcg tcacgccttc
     1261 tggaaagcag ctgtttctcc atgcacaaac cattctccaa gagatttcta ctgcaagagc
     1321 tgaaatcact cagttgcaaa agaaaaagct tcgttttggc atgcctccta ttatcggcaa
     1381 tgaatacttt cctaaactgt caagttactt tatcaagcat gaattgatga atcaaattga
     1441 gattaccgat ggaggctctc gggacttata cgggatgatc cgacaaggga ggatcgattt
     1501 agctgtatta ggctcaacac agccaatcgt ggatcaatat ttagaaacag agttactttt
     1561 ggacaaacgt ttcatgatcg tcgtgccacc tacacatcct ctttccaagc aggatactgt
     1621 ctctttcaaa gatatagcag atgaacagtt tgtactattg aacgaacatt atgtccaccc
     1681 cgcagcattc actaagcttt ctaaacaaac acatactcat ccaaaagttg tttatcaaag
     1741 caatgacttg acgattttaa agagtatggt ccgagaaaat gtgggtatcg gttttttggc
     1801 agagattgct gtccatcctg aagatcattt agtttgcctc cctctttcag atgaagttca
     1861 gcctcgcttc ttgatttcat tggcaaaacg aacacagcag atcgtcacgc ctcttcaaga
     1921 acaagtagtc caagttatcc atgaattcgt tacagcacaa aaaaacagct cttcgtaaaa
     1981 gaagagctgt ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa
     2041 acgatgccgc gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg
     2101 cctttagcac caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa
     2161 gtcaatccgc cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct
     2221 ttgtcagttg atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca
     2281 gcagatgaag caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct
     2341 aataatttag aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact
     2401 ggtacaccag cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct
     2461 tttttagctg ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg
     2521 taaacgcccc agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag
     2581 atatctgcat ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca
     2641 gcaacgattg ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt
     2701 tcagttacat ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca
     2761 ttttcagctt cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg
     2821 tttgcagatt caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg
     2881 tcagaagctt ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta
     2941 gcagtgataa ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt
     3001 ggtactagtt cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca
     3061 gaaactttga tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag
     3121 atttgaacat gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt
     3181 gctttacgta caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata
     3241 tcgtcagcat cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc
     3301 cctttacgtg atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct
     3361 tcgtcttttt caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca
     3421 acgtgaacat cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca
     3481 tgttcaggat ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca
     3541 cgaccaaaat cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta
     3601 tctaaaagaa taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata
     3661 cgtgataatt gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca
     3721 gattcaatca attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg
     3781 attttggttt ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac
     3841 acagcttaaa tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata
     3901 tagtgataag tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac
     3961 gatttcgttg tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac
     4021 agcgtaagca ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg
     4081 gacaacgtgt cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc
     4141 agcgaattca tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc
     4201 gcgatcgcgg ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg
     4261 gatgatgatt tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt
     4321 gcgtcccatt acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg
     4381 gtcaattgat tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc
     4441 aggaatatca ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa
     4501 agccattgcg ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa
     4561 ttttttcaat tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg
     4621 tgcagaatac aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag
     4681 acgacgaata tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag
     4741 tccttcgtaa atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc
     4801 tccgccactg gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact
     4861 ttatcctaag tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta
     4921 acattatttt tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa
     4981 gtagtatttt ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata
     5041 agttgcccct ttatactctt gctgtcatcc acccaataag catctgacag aactactttt
     5101 cgttgcgttt tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc
     5161 acttgtaatt gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa
     5221 gtttgtgaag gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt
     5281 tgcagttcct gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca
     5341 acggcaaaca aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct
     5401 ccactactgt cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct
     5461 ctaacttcct tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc
     5521 agctgaactt gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa
     5581 taaataccta agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact
     5641 tcttcttctt ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata
     5701 ttttggattt ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca
     5761 aaaactccta ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc
     5821 cgtatgagga attgatccac tgactggaac gggccatttt gttttctttc gtttaatata
     5881 tcttctacaa aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc
     5941 ttattgacca actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt
     6001 ttatttttac gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa
     6061 ttcatcaggc tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat
     6121 cccacgaatg aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg
     6181 atatattcat atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat
     6241 acaaaatgcg acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc
     6301 agaatatctg actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt
     6361 tcctgataga cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg
     6421 tagcgaatgg cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg
     6481 cctggtcgat acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt
     6541 cgtaaaacgt tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt
     6601 tgaaacaaga gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac
     6661 ccttctactc gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct
     6721 aaaaagtcca ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga
     6781 aaagcttcat cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata
     6841 actacacctg ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt
     6901 ttgaataaaa gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt
     6961 ttataagctt gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt
     7021 gcttcacttt gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc
     7081 attgtgccaa atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa
     7141 ttcaatactt cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag
     7201 cgttcggggt ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg
     7261 atttctaaca cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg
     7321 attttgtttc tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat
     7381 cccatttgat ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag
     7441 tcagtgattc ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt
     7501 tttccattag gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa
     7561 atcgattcag ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat
     7621 cgtttgatca tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca
     7681 gataaatttt ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca
     7741 aaaagttctt cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct
     7801 tttatcacat actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata
     7861 taaaaatttc tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc
     7921 tctttattga aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg
     7981 gtttctgtca tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc
     8041 atcagctgct gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct
     8101 tcataagtat aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata
     8161 cgatagaatt caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag
     8221 ccttgttttt ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa
     8281 taagaagtaa ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt
     8341 aaaattcact agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga
     8401 agttgattat tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag
     8461 aactcaaatt ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa
     8521 ttaagataga aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt
     8581 tctaggtcaa gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac
     8641 tttgacaaca atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc
     8701 gccaagaaaa gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca
     8761 agctcttttt tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga
     8821 aagttgaata aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca
     8881 gcactgtaat tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca
     8941 aagttgctaa acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc
     9001 ttactgatca gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag
     9061 cgaagtatac ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat
     9121 gataaaatga gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct
     9181 agtttcggtg aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca
     9241 aaaaggatat tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg
     9301 atcgtagtga ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag
     9361 agatgatcca tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga
     9421 tggaaatctt ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct
     9481 gtgatcaaga atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt
     9541 ccgatcagat ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt
     9601 tctctaggtg cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc
     9661 ccattccaaa accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc
     9721 attgttaatg tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca
     9781 gctagatttc cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct
     9841 agtcccgaaa acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt
     9901 atgaatggca taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc
     9961 actaaatttt ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata
    10021 cctattttcg aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg
    10081 tcattttata tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt
    10141 ccaatccctt ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat
    10201 atcaacgatc cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat
    10261 tgtatagaaa ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa
    10321 gatcgatgca aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa
    10381 agaagccatc atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac
    10441 aatggcgatc agtggagaga ataaactact taatgcgaat agcactgcca ctactactga
    10501 agtcaaacca gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt
    10561 attcgatgta ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt
    10621 atccattttt gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa
    10681 aatacctgtg cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa
    10741 actgaatgcg atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga
    10801 ttgcatgcct tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt
    10861 acctaacgaa ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc
    10921 aaggactgtg gtcactacga taccgataag aatagcccca cggacatttt ttacaactaa
    10981 aatggctgtc agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc
    11041 taacgcaggg acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac
    11101 agagctcgaa atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc
    11161 gacatacgca acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat
    11221 cgcccgaata atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca
    11281 aataaacacc attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa
    11341 agtgaaaaat gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa
    11401 tcccattacc agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa
    11461 gggcatacct gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa
    11521 gaatgtcgtt actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc
    11581 gaaaaacttt tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat
    11641 aataaaaaat aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt
    11701 cattcgcttt tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt
    11761 tagcttatgt ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt
    11821 gctcttgtta attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg
    11881 acccttattg aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga
    11941 ttcgatgatt acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca
    12001 tgtcagtatt gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga
    12061 attaacaaca ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg
    12121 ggatggtgaa agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa
    12181 aaagcagcta aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag
    12241 ttttgatttc tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct
    12301 ttctcctaaa aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt
    12361 tgcaggagct gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac
    12421 atggggtgga cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg
    12481 cgtgcaagag attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga
    12541 cgagcataat gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg
    12601 tactgaccaa ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg
    12661 tttagctgtt tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa
    12721 agaggttgtg acaatttttg tcacaacctt ttttgccgag taaacggtgt tcaaaagcca
    12781 gtttctcggt atgaagtcaa atttaagcaa aaatgtgaga agctattttc acaaaatttt
    12841 tcttcatact cgaaactaag cggctttttc acgacctctt ttttattttg ccatgaacat
    12901 atcataatac gcgtccatta tttctttagc aatcgtcaag tttatgtggt cttgttcgtc
    12961 taataagttc ggcaagacaa cactgatggc aatttccgga ttgtctgttg gtccgtatgc
    13021 cacgatatta ctattgactg tcgcaatatc tggatgacct tccgcaaccg tttctgcagt
    13081 ccctgttttt gcggaaaggt ccattttcgc actggctaat ggtttggctg ttgtgtaagg
    13141 atcggtacca tggacaactt ggtggaatcc ttcacggagg atcgccatat tttctgcaga
    13201 aatattgacc gtattttcta ctgttgtgcc aatttctttc ttcacttcac ccaagtttcc
    13261 attttcgtca ttgccataga tcgctttgac taggtgcggc tgtactcgtt tcccgccatt
    13321 agcgactgtt gaagcatatt gagctaattg gattggtgta tatgtgtcaa actgtccaaa
    13381 tgccaaatcg gtaaagttac ctggagcata ccattctttg ccattatccg cattgaactt
    13441 cttcatataa tcaacagaac gtgagatccc aggagattca ttcggcaaat cgatcccagt
    13501 cgttgtacca agaccaaact ctttgaaaga gtctctcaat tgctgataag ctttagcttc
    13561 ttcatctaaa ctaggtagtc ccattccagg tgtatagtct agtcctaaca tctttaacgc
    13621 cactttgatc atatacgtat tagaagacaa ttccgatgct ttgactgcat caagagcaac
    13681 ttgtccgctt cgattaaaga cagagctttt ttctgaagca ccttgcagac gaatcggctc
    13741 atcaatcaaa acttgatttc cgctgatcgc gttacttgcc cagcctgctg tcaaagtacc
    13801 agccttgact acagaccctg gatcaaaggc gctagtaatc gtgcctaaag cattctcggt
    13861 gatttctttg gaaccttgtt catgactgaa accagtcatt gctagcactt ctcctgtctg
    13921 aggattcata gccactgcat aagcaccagg cgaatactgt gctttgccgt tgttgatcaa
    13981 tgtttgataa ttccttttca aaatttcttc tacttttgat tggaattctg cattcataga
    14041 taacattaaa ttagaacctt tttccccaga gaagatttct ttttgattcg aaacatttcc
    14101 ttcattatca agggaaactt catattgggt tttggttcct tgcaggacat cttcatattg
    14161 tttttccaaa taactttgtc ctacacggtc attccgagaa taaccttttg ctaggtattt
    14221 ctctgcttca tctgctggaa gtccttgctt ttcagtcgtc acactgccaa gaatactttt
    14281 cagtgaactt gctgcattat attctcgtgt ccagtctgtg ccagtcgaaa ctcctggaag
    14341 ttcagaagcg cgttctgcca caacagctaa ctcttgatca gtaacaccag agtttttgat
    14401 atagacagtg tttaattcat atgcactatt catccgttta aagatggtag caatttttaa
    14461 ttgctcctcg tttaaattaa tctcatcagc tgtgacttta ttgaccatcg cactatattg
    14521 ttcgctgttc cctaattgct tttcttttgc cgaaagtctt tttgtggctt ctttcaaatg
    14581 tttgggatca gccagccaaa aatctttttt atctctatct gtcagatttt catctaccgg
    14641 aacatcgatc agcgagttca gcttcgttgc gatttcaagc aaatctttag ccgtaacttg
    14701 tgttcctctc gtatatgtga tggcctgatt cgctttattc gtaactaaag cttttcctgt
    14761 ggagtcgtag atcattccgc gaggagtact ttcttggacc gtaatcgtag aagtagactt
    14821 cactcgcgcc tccatctctt cgccgttcac gatctgcaaa taacccaatc tagcaatcaa
    14881 cgcaacaaat aacgtaaata taatgaaaaa aagaaaattt aagcgaaaag gaacatggga
    14941 ccggcgaaac gttttgttct taggttcttg tgcacctttt cttatttttt ctaa
//
LOCUS       DACEMK010000065        14872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEMK010000065 DACEMK010000000
VERSION     DACEMK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.067"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(16..834)
                     /gene="purR"
                     /locus_tag="IW715_001830"
     CDS             complement(16..834)
                     /gene="purR"
                     /locus_tag="IW715_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1022435.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(1001..1825)
                     /locus_tag="IW715_001831"
     CDS             complement(1001..1825)
                     /locus_tag="IW715_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1022436.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(1798..2478)
                     /locus_tag="IW715_001832"
     CDS             complement(1798..2478)
                     /locus_tag="IW715_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022437.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(2501..3439)
                     /locus_tag="IW715_001833"
     CDS             complement(2501..3439)
                     /locus_tag="IW715_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1022438.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(3684..4535)
                     /locus_tag="IW715_001834"
     CDS             complement(3684..4535)
                     /locus_tag="IW715_001834"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1022439.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(4689..7262)
                     /gene="mprF"
                     /locus_tag="IW715_001835"
     CDS             complement(4689..7262)
                     /gene="mprF"
                     /locus_tag="IW715_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1022440.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7403..8209
                     /gene="proC"
                     /locus_tag="IW715_001836"
     CDS             7403..8209
                     /gene="proC"
                     /locus_tag="IW715_001836"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1022441.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(8288..9844)
                     /gene="rny"
                     /locus_tag="IW715_001837"
     CDS             complement(8288..9844)
                     /gene="rny"
                     /locus_tag="IW715_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1022442.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10255..11304)
                     /gene="recA"
                     /locus_tag="IW715_001838"
     CDS             complement(10255..11304)
                     /gene="recA"
                     /locus_tag="IW715_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1022443.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(11413..12654)
                     /locus_tag="IW715_001839"
     CDS             complement(11413..12654)
                     /locus_tag="IW715_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1022444.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(12894..13472)
                     /gene="pgsA"
                     /locus_tag="IW715_001840"
     CDS             complement(12894..13472)
                     /gene="pgsA"
                     /locus_tag="IW715_001840"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1022445.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(13681..14565)
                     /locus_tag="IW715_001841"
     CDS             complement(13681..14565)
                     /locus_tag="IW715_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1022446.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         4265 a         3303 c         2586 g         4718 t
ORIGIN      
        1 ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa tatttttcgt
       61 ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg cgcgatgacc
      121 gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga attcttcaat
      181 caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca ctacaaggac
      241 acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc tttcagaaga
      301 tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt cccggcgtac
      361 aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta cgccttttgt
      421 ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa tgattcttcc
      481 aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat atcctcctgg
      541 aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt ttttcgcctc
      601 ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga ttgtttccaa
      661 ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt cactgataga
      721 agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg
      781 attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt tcacctattc
      841 caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc aaaaattcga
      901 gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa gaagcctata
      961 aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt tttcttctga
     1021 aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata aagactaatg
     1081 tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct gacagcatcc
     1141 ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg cctaatcgca
     1201 taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg gcaatcggga
     1261 tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc aaatgaacgg
     1321 gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc ggccgtttga
     1381 ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg acttgctccc
     1441 atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct gaattccctc
     1501 cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa atggcaatgg
     1561 agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca gctgctaata
     1621 caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct aacgctaccc
     1681 ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg atcactaaaa
     1741 acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac gctcttctca
     1801 tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc ggatctgtct
     1861 gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg atttcccatg
     1921 gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt catccatacc
     1981 tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac gcgctaaact
     2041 gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca tatcccacat
     2101 ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga gacgcttgaa
     2161 ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac taggaaaacc
     2221 tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt ttccttcttg
     2281 atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta acgatgcttt
     2341 caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt cacccgcatc
     2401 tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga
     2461 tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata ctttctttta
     2521 aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg atttgttcat
     2581 tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca ttcgacaatg
     2641 tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg ttgtttttct
     2701 tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc
     2761 ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct
     2821 gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt tgatccagtg
     2881 cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt ttatctggat
     2941 atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat accggatcga
     3001 gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt
     3061 cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc atcaaggtct
     3121 gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct gaactattat
     3181 acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt tcgaaatcat
     3241 gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct acgatctctt
     3301 ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct tttttgtctg
     3361 cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt accgtagctg
     3421 tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa ttttacgatt
     3481 tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat ccatcaattt
     3541 ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc
     3601 tagccatttc actacaataa aaaactgaag aataccattt taggattcct cagtttttca
     3661 tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac ttcctcacaa
     3721 aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca caaggcaaaa
     3781 acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat catacgctct
     3841 ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact gtttcccatt
     3901 tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc gtggatgtct
     3961 tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga gacactcatt
     4021 cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg gatttcttcc
     4081 cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc tgtaccgact
     4141 tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa ttggttgatc
     4201 cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc tactggaata
     4261 ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt tagcaataaa
     4321 gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt tgtttctaca
     4381 atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac
     4441 atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc cagtcctaaa
     4501 tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg aaaagtctaa
     4561 gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc ctagtggttg
     4621 attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca aacaagttct
     4681 agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt tcctttttct
     4741 tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc tttcctataa
     4801 gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata agcagactgg
     4861 aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc ttgaaagaaa
     4921 gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc ttccgagtag
     4981 cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa ttcctcagat
     5041 tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat cggctgagcg
     5101 gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct agataataca
     5161 tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg aagccatttt
     5221 tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa
     5281 tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg agacaagtct
     5341 tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa acccaattca
     5401 tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata gcctaaacaa
     5461 tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt gccaactgga
     5521 tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac ttgatcttgg
     5581 tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg gcttgtagct
     5641 gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc actgccaagc
     5701 ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa tgagatgccc
     5761 aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa atgatttcct
     5821 gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta aacgataaac
     5881 aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac gaattttgtt
     5941 cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata taccaatatc
     6001 aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag taaagcaatc
     6061 gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc gagtaataaa
     6121 aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg caataatact
     6181 tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac catcatgatc
     6241 cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc
     6301 ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa caatagaagt
     6361 cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca aactactgct
     6421 acatcttgcc gaacagacaa ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc
     6481 attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa cataggatag
     6541 atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa taccgcaagg
     6601 gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt tggataaaac
     6661 tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa
     6721 ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg aataaaaaag
     6781 acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa tagtgcgact
     6841 tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa actagcccgc
     6901 agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt tgttacccac
     6961 gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc taagactctg
     7021 atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc cagaatcatg
     7081 agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg ccaactcatg
     7141 ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc gaaaaaaatc
     7201 agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg tttatttttc
     7261 acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta tcttatttat
     7321 tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag aaagatatcc
     7381 taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga atatggcaca
     7441 agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc
     7501 cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa
     7561 aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga aaccaaaaat
     7621 cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat tcatttctat
     7681 tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc aggcctccat
     7741 cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa
     7801 aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg aagcaatcgg
     7861 cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg ctggcagctc
     7921 acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac tgaatggttt
     7981 gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt gtcaaaaatt
     8041 cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc ctggcggtac
     8101 aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa tcatcaaagg
     8161 aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat gatagcaatg
     8221 ttttttcgta ccttacaaaa aaagaagagc tgtggctttt gccgcggctc ttcttttgta
     8281 ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt actttgatgt
     8341 gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact aaacgaacag
     8401 cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt ccagcttgaa
     8461 ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga cttcttaaac
     8521 gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct gagagcgcat
     8581 ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca tgatgagaag
     8641 cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc gcaccgatct
     8701 caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg agcaacccgg
     8761 cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca gctaattttg
     8821 caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat cgcagacgtc
     8881 cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac gctgcctctt
     8941 ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc atttcttcga
     9001 tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc cgtgcgattt
     9061 ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca tcgatgatta
     9121 aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt ccaatgatac
     9181 gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct gaaacttgat
     9241 ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga tctgcttctt
     9301 ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc agctcttctt
     9361 cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca attttttcta
     9421 gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct tcaattaatt
     9481 gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt ttttctaacg
     9541 agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct tgagatttta
     9601 attctaactt actttctctc agctcacttt caacttcaga acggtatttc tgattttctt
     9661 ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt gcactatcga
     9721 tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga
     9781 ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga atgttgaata
     9841 ccataaaatc cacctccata ctatcttttt catttttatc atctatacag cttctatgat
     9901 aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa aacatgcaca
     9961 actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag aaacggcttt
    10021 ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat ataggtaaac
    10081 ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga tatttttatt
    10141 ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata aaaaaacggc
    10201 aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata atttttattc
    10261 ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga cttcttcacc
    10321 aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag gatgattagc
    10381 catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat aagaatacca
    10441 agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt ctccttcttg
    10501 tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag gtgctacttt
    10561 gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt gtttcaattg
    10621 ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg
    10681 agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt tgatgaaaat
    10741 agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt gagacatcaa
    10801 acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg ctcgtggaac
    10861 taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata ctaaagcatc
    10921 agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt
    10981 cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa tgaaagcggc
    11041 cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg ttttacctga
    11101 actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga cacctaaagc
    11161 gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga ttttttcgcc
    11221 caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg cagcatctaa
    11281 ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa atcagagcaa
    11341 catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa
    11401 tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc cttgcataac
    11461 cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg aaaccacgcc
    11521 gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta acgtgtccgg
    11581 tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac gtgcttgaat
    11641 ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata gctccggatc
    11701 aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc ctggaaatac
    11761 ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg tcaaactttc
    11821 cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt ttgctagcga
    11881 attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt tttcttctaa
    11941 tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt
    12001 cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct caatcagatc
    12061 ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa ctttagaaat
    12121 tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt ccacaaacat
    12181 tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat ctgtttgata
    12241 aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt cgataatctg
    12301 ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat atgccaataa
    12361 ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg cagttgcttc
    12421 ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga gttcacttct
    12481 tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta caactgtttg
    12541 ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg cattcgtatt
    12601 cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt tcattacttc
    12661 ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa agagggcaag
    12721 gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt tctcctgcca
    12781 aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt tgaaattatc
    12841 ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga tacttacatt
    12901 gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata gatagtaaag
    12961 atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct gaatgggata
    13021 ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac ttttcccggc
    13081 caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc tgtgacggct
    13141 aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg gtcaaccaac
    13201 atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa
    13261 ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt gatactagct
    13321 aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc tcctacgtta
    13381 aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat catacaaatt
    13441 ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt ccctattcta
    13501 cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa tttattcaaa
    13561 ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc gacacagatt
    13621 tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta atttatacga
    13681 tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt tttcccctgg
    13741 attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg caccaatcgt
    13801 gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat ttgctgccag
    13861 tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac gattgacagc
    13921 agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga tggcactgcc
    13981 agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg ttgttgattc
    14041 tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact cctcgactga
    14101 tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt gacgatcttg
    14161 ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta ataaaatgac
    14221 aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg cttttctaga
    14281 agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt tgcttttccc
    14341 atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt attgccgaat
    14401 aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc
    14461 caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata tttttttatt
    14521 caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt catcttctcc
    14581 agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg tttgggtgct
    14641 gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc ctgccaactc
    14701 ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa cttgttcctt
    14761 gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt caatcagctg
    14821 ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc gt
//
LOCUS       DACEMK010000066        14567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEMK010000066 DACEMK010000000
VERSION     DACEMK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.068"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IW715_001842"
     CDS             complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IW715_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1022447.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1993..3216)
                     /locus_tag="IW715_001843"
     CDS             complement(1993..3216)
                     /locus_tag="IW715_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1022448.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3247..4086)
                     /locus_tag="IW715_001844"
     CDS             complement(3247..4086)
                     /locus_tag="IW715_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1022449.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4103..4783)
                     /locus_tag="IW715_001845"
     CDS             complement(4103..4783)
                     /locus_tag="IW715_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1022450.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4785..5858)
                     /locus_tag="IW715_001846"
     CDS             complement(4785..5858)
                     /locus_tag="IW715_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1022451.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6505..7176)
                     /locus_tag="IW715_001847"
     CDS             complement(6505..7176)
                     /locus_tag="IW715_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1022452.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IW715_001848"
     CDS             complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IW715_001848"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1022453.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10600..11037
                     /gene="ndk"
                     /locus_tag="IW715_001849"
     CDS             10600..11037
                     /gene="ndk"
                     /locus_tag="IW715_001849"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1022454.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11059..11925)
                     /locus_tag="IW715_001850"
     CDS             complement(11059..11925)
                     /locus_tag="IW715_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1022455.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12085..14178)
                     /locus_tag="IW715_001851"
     CDS             complement(12085..14178)
                     /locus_tag="IW715_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1022456.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4229 a         3109 c         2480 g         4749 t
ORIGIN      
        1 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
       61 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
      121 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
      181 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
      241 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
      301 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
      361 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
      421 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
      481 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
      541 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
      601 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
      661 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
      721 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
      781 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
      841 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
      901 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
      961 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     1021 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     1081 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     1141 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     1201 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     1261 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     1321 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     1381 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     1441 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     1501 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     1561 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     1621 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     1681 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     1741 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     1801 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     1861 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     1921 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     1981 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     2041 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     2101 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     2161 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     2221 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     2281 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     2341 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     2401 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     2461 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     2521 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     2581 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     2641 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     2701 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     2761 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     2821 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     2881 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     2941 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     3001 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     3061 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     3121 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     3181 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     3241 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     3301 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     3361 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     3421 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     3481 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     3541 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     3601 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     3661 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     3721 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     3781 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
     3841 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
     3901 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
     3961 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
     4021 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
     4081 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
     4141 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
     4201 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
     4261 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
     4321 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
     4381 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
     4441 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
     4501 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
     4561 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
     4621 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
     4681 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
     4741 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
     4801 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
     4861 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
     4921 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
     4981 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
     5041 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
     5101 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
     5161 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
     5221 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
     5281 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
     5341 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
     5401 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
     5461 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
     5521 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
     5581 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
     5641 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
     5701 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
     5761 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
     5821 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
     5881 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
     5941 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
     6001 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
     6061 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
     6121 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
     6181 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
     6241 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
     6301 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
     6361 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
     6421 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
     6481 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
     6541 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
     6601 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
     6661 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
     6721 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
     6781 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
     6841 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
     6901 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
     6961 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
     7021 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
     7081 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
     7141 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
     7201 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
     7261 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
     7321 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
     7381 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
     7441 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
     7501 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
     7561 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
     7621 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
     7681 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
     7741 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
     7801 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
     7861 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
     7921 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
     7981 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
     8041 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
     8101 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
     8161 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
     8221 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
     8281 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
     8341 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
     8401 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
     8461 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
     8521 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
     8581 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
     8641 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
     8701 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
     8761 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
     8821 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
     8881 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
     8941 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
     9001 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
     9061 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
     9121 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
     9181 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
     9241 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
     9301 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
     9361 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
     9421 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
     9481 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
     9541 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
     9601 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
     9661 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
     9721 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
     9781 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
     9841 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
     9901 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
     9961 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    10021 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    10081 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    10141 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    10201 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    10261 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    10321 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    10381 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    10441 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    10501 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    10561 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    10621 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    10681 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    10741 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    10801 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    10861 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    10921 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    10981 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    11041 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    11101 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    11161 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    11221 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    11281 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    11341 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    11401 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    11461 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    11521 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    11581 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    11641 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    11701 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    11761 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    11821 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    11881 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    11941 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    12001 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    12061 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    12121 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    12181 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    12241 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    12301 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    12361 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    12421 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    12481 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    12541 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    12601 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    12661 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    12721 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    12781 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    12841 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    12901 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    12961 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    13021 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    13081 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    13141 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    13201 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    13261 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    13321 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    13381 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    13441 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    13501 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    13561 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    13621 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    13681 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    13741 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    13801 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    13861 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    13921 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    13981 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    14041 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    14101 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    14161 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    14221 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    14281 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    14341 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    14401 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    14461 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    14521 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattg
//
LOCUS       DACEMK010000067        14294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEMK010000067 DACEMK010000000
VERSION     DACEMK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.069"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..2604
                     /locus_tag="IW715_001852"
     CDS             133..2604
                     /locus_tag="IW715_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1022457.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2707..4443)
                     /locus_tag="IW715_001853"
     CDS             complement(2707..4443)
                     /locus_tag="IW715_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1022458.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4440..5144)
                     /locus_tag="IW715_001854"
     CDS             complement(4440..5144)
                     /locus_tag="IW715_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1022459.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5275..5778
                     /locus_tag="IW715_001855"
     CDS             5275..5778
                     /locus_tag="IW715_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1022460.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5738..6076
                     /locus_tag="IW715_001856"
     CDS             5738..6076
                     /locus_tag="IW715_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1022461.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6097..7515
                     /gene="ffh"
                     /locus_tag="IW715_001857"
     CDS             6097..7515
                     /gene="ffh"
                     /locus_tag="IW715_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1022462.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7627..8205
                     /locus_tag="IW715_001858"
     CDS             7627..8205
                     /locus_tag="IW715_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1022463.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8209..8730
                     /locus_tag="IW715_001859"
     CDS             8209..8730
                     /locus_tag="IW715_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1022464.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8809..9363)
                     /locus_tag="IW715_001860"
     CDS             complement(8809..9363)
                     /locus_tag="IW715_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1022465.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9616..9891
                     /gene="rpsP"
                     /locus_tag="IW715_001861"
     CDS             9616..9891
                     /gene="rpsP"
                     /locus_tag="IW715_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1022466.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9903..10154
                     /locus_tag="IW715_001862"
     CDS             9903..10154
                     /locus_tag="IW715_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1022467.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10279..11070
                     /locus_tag="IW715_001863"
     CDS             10279..11070
                     /locus_tag="IW715_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022468.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11240..11761
                     /gene="rimM"
                     /locus_tag="IW715_001864"
     CDS             11240..11761
                     /gene="rimM"
                     /locus_tag="IW715_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1022469.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11751..12512
                     /gene="trmD"
                     /locus_tag="IW715_001865"
     CDS             11751..12512
                     /gene="trmD"
                     /locus_tag="IW715_001865"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1022470.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12648..12995
                     /gene="rplS"
                     /locus_tag="IW715_001866"
     CDS             12648..12995
                     /gene="rplS"
                     /locus_tag="IW715_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1022471.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13253..13546
                     /locus_tag="IW715_001867"
     CDS             13253..13546
                     /locus_tag="IW715_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1022472.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4795 a         2567 c         2905 g         4027 t
ORIGIN      
        1 tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
       61 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
      121 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
      181 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
      241 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
      301 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
      361 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
      421 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
      481 ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata
      541 gatatagaag accttgtaat agatgacatt gttattttag agactggata tcaaattcca
      601 gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa
      661 gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc
      721 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
      781 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
      841 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
      901 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
      961 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     1021 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     1081 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     1141 aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     1201 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     1261 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     1321 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     1381 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     1441 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     1501 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     1561 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     1621 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     1681 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     1741 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     1801 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     1861 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     1921 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     1981 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     2041 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     2101 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     2161 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     2221 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     2281 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     2341 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     2401 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     2461 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     2521 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     2581 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     2641 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     2701 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     2761 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     2821 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     2881 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     2941 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     3001 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     3061 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     3121 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     3181 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     3241 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     3301 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     3361 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     3421 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     3481 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     3541 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     3601 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     3661 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     3721 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     3781 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     3841 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     3901 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     3961 ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     4021 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     4081 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     4141 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     4201 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     4261 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     4321 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     4381 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     4441 catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg
     4501 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     4561 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     4621 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     4681 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     4741 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt
     4801 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     4861 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     4921 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     4981 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     5041 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     5101 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     5161 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     5221 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     5281 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     5341 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     5401 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     5461 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     5521 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     5581 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     5641 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     5701 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     5761 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     5821 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     5881 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     5941 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     6001 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     6061 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     6121 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     6181 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     6241 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     6301 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     6361 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     6421 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     6481 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     6541 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     6601 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     6661 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     6721 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     6781 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     6841 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     6901 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     6961 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     7021 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     7081 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     7141 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
     7201 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
     7261 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
     7321 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
     7381 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
     7441 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
     7501 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
     7561 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
     7621 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
     7681 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
     7741 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
     7801 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
     7861 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
     7921 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
     7981 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
     8041 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
     8101 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
     8161 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
     8221 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
     8281 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
     8341 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
     8401 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
     8461 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
     8521 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
     8581 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
     8641 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
     8701 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
     8761 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
     8821 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
     8881 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
     8941 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
     9001 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
     9061 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
     9121 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
     9181 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
     9241 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
     9301 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
     9361 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
     9421 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
     9481 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
     9541 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
     9601 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
     9661 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
     9721 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
     9781 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
     9841 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
     9901 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
     9961 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    10021 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    10081 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    10141 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    10201 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    10261 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    10321 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    10381 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    10441 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    10501 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    10561 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    10621 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    10681 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    10741 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    10801 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    10861 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    10921 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    10981 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    11041 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    11101 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    11161 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    11221 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    11281 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    11341 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    11401 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    11461 tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct
    11521 agctgaaaat gaattctatt accatgagat catcggtctg catgtattag aagatggaac
    11581 tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca
    11641 acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga
    11701 tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg
    11761 atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta
    11821 aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata
    11881 acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag
    11941 tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa
    12001 gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt
    12061 cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc
    12121 gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag
    12181 gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga
    12241 cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc
    12301 cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga
    12361 ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt
    12421 tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag
    12481 aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg
    12541 ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg
    12601 ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa
    12661 tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca
    12721 ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag
    12781 gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt
    12841 ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg
    12901 aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg
    12961 gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
    13021 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
    13081 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
    13141 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
    13201 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
    13261 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
    13321 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
    13381 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
    13441 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
    13501 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
    13561 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
    13621 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
    13681 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
    13741 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
    13801 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
    13861 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
    13921 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
    13981 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
    14041 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
    14101 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
    14161 aagcaatact tcaattaatc aagttttatc tttttttttc aataatcgta aaaaaagagt
    14221 attttttatt taaaaataaa atctttgtat taaaggacaa gactaaaata accttagaat
    14281 aaaaagagaa aaaa
//
LOCUS       DACEMK010000068        14195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEMK010000068 DACEMK010000000
VERSION     DACEMK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.070"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1127)
                     /locus_tag="IW715_001868"
     CDS             complement(<1..1127)
                     /locus_tag="IW715_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1022473.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1210..1545)
                     /locus_tag="IW715_001869"
     CDS             complement(1210..1545)
                     /locus_tag="IW715_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1022474.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1555..1929)
                     /locus_tag="IW715_001870"
     CDS             complement(1555..1929)
                     /locus_tag="IW715_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1022475.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1942..2256)
                     /locus_tag="IW715_001871"
     CDS             complement(1942..2256)
                     /locus_tag="IW715_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1022476.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="IW715_001872"
     CDS             complement(2369..5596)
                     /gene="fss3"
                     /locus_tag="IW715_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1022477.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            6384..6668
                     /locus_tag="IW715_001873"
     CDS             6384..6668
                     /locus_tag="IW715_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1022478.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            6671..7588
                     /locus_tag="IW715_001874"
     CDS             6671..7588
                     /locus_tag="IW715_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1022479.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            7581..8363
                     /locus_tag="IW715_001875"
     CDS             7581..8363
                     /locus_tag="IW715_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1022480.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            8364..9044
                     /locus_tag="IW715_001876"
     CDS             8364..9044
                     /locus_tag="IW715_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1022481.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            9037..9933
                     /locus_tag="IW715_001877"
     CDS             9037..9933
                     /locus_tag="IW715_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1022482.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            10128..11777
                     /locus_tag="IW715_001878"
     CDS             10128..11777
                     /locus_tag="IW715_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1022483.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            12371..13306
                     /locus_tag="IW715_001879"
     CDS             12371..13306
                     /locus_tag="IW715_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022484.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            13299..14069
                     /locus_tag="IW715_001880"
     CDS             13299..14069
                     /locus_tag="IW715_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1022485.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         4184 a         2724 c         2831 g         4456 t
ORIGIN      
        1 tgatcgcgga ttccgtctcc taaatatttc gcatccggcc tttggcaagc caaaatcaga
       61 aagaaaccag attgacgacc caacatcacg atttgtttga gcttattcaa aatcaccgca
      121 ctttctttcg tcgttaacat ctccatataa gccacgtatt catcaaagat taaaaagttg
      181 ggtggaagtc caagataggc ataattctct cctgttttgt agttagacat ttctttcatt
      241 gccttgctac gagccatcat acgttcatag aaatcttcca cacaagcaga aatttcttcc
      301 ttttgtgaat aaacatgagg catcaccgta cctaaatcag ctaaatctgc atttttggga
      361 tcgaggataa acaattctgc atctgacttc aatagtgctt cgataatggt gaggaggaaa
      421 taggtcttcc ctccacccgt gccaccagcg attaacatat ggggtaaaga atcataagcc
      481 catacttgat ttttcatcaa tcgcagagtc ccattttctg ccactacttc gtctatccca
      541 attcgattgg cgatcatatc atacagtaaa gtatactcca cataggagtc tttgagttcc
      601 ttttctacta actcacaata cagtccgctt tctaacttct tttccaattt taaaagctga
      661 tcttggtatt tcccaagtga aatttgaacg cgaatagaca gtaatccatc tttcattcga
      721 taatagattt tggggaagta actgatagtc tctctggttc ggctactatt caaatctttg
      781 aaaaagcctt cactttgagt ttgtttcact tcataccaat ggttctctag aatcatccga
      841 gctagctttt gtcgatgcca caagcttcgc caatgatcaa gaaaaaagtg aatataaagc
      901 aatatcatag ctcctgaaaa agcgagactt ccaagcagac atactaaatc ttttatagac
      961 caaacgattt caatagtaga aagtgatagc ttagaaatat caaattgtct aaactcttga
     1021 aaataaaaga cgctcatcca accgatgaac agagcgagta gccagccaag aacaaagtga
     1081 taaaccaagt gctgatctcc tgcgcgtatg cgatgtcctt tgtacatttt gatggtttcc
     1141 acctccaatc gaaaaagccc aataggattt cattaggcaa tttcaaaaag ttatgctagt
     1201 catttactgc tacgaggagg ggagttgttt cgtttcaacc aattgattta aaaagccttc
     1261 aatagcatcc ggcatctcca caccaatttc ttccaaaatg gattctgcct gccgacataa
     1321 gttttcattt acttcgacaa ctataaagaa agactcttct tctcctaacc agtctagtac
     1381 atcagagtct tcttcactgt cgctattttc tccaagtaaa ccttgatgta taatcagaaa
     1441 cattttttca tcagaggtta atgctttaac gagttgttcc tcagtcagct cgccttttcc
     1501 atattgatcg agacaatcta aaatatgttc aacattaatt tccataatct tcacctattt
     1561 ctttctttca ttttggttcg taggatttcc ttgattcggc tgccgcttca agactaaatc
     1621 atccgcctta atgaaccaat taacggtagt tccttctcga aaaacataat tggcaacggt
     1681 atccacgatt ggattgacca actctacctc tgcatcataa gcaaactcgc gtaagggtac
     1741 attagctggg atactaactt gaatcattcg cccttgtgca gaggatttta aattataggt
     1801 tcgttctttt acctcattac caattgttcc atcctcattt tgcttccgta cttctcggcg
     1861 taatgcggaa aaacgcaact tcccaaatgt taaacctgaa tctacgacaa taccttctgt
     1921 taatctcatc ttcttttcct cctatgcttt cactaaatca tcaacataca acacgtaatc
     1981 agtaaaccca cgattctcaa tctcatagcc tttggcaacc aaatggggat tcacggcttt
     2041 tagcggttgg tcttgatcaa aatccttttc accggcttcc gcagctacta ccacctcaat
     2101 atcatcggcc cgttgcttac tggaatacaa atgataactg cgaccaatca ccgtagtatt
     2161 ccgtccatat ccttctgtta taatttctcc ttcaccggca taacttaact ttccaaaggt
     2221 ttcagccata tctggcacga caaatttcaa ttccatcttc ttttcctctt ttcattgtta
     2281 gttttggata caaaaatagc gcgactactt ttttaggagg cggtcgcgca cgttggttct
     2341 taggatttac ttgtatcatc tcatcacctt agttccggcg attccagaaa taatagcccg
     2401 ccgtcgcaaa aatcaaagta aaaccaatga acagtaaaac attggaattt ttctccccgg
     2461 tttgagggaa ggtcttcact gtgggactag attcttgaga gccacttgga acagttggtg
     2521 tttccggttg tttcggcgta cttagtatgg ttggaacttc ttttggtgtt aaggtttgag
     2581 atttttcttt caaatcttca ttgtgttttc cattgatatt cccatcttta tcgtaattga
     2641 tttccgcaaa agtaaacttg gttccttctg gatattttcc ggtatctact tttttcgcaa
     2701 gcacagtttt ggtgaattct ttatcattta cttcataatc aatcttgcca gatttccaaa
     2761 tttctttgtt tgtaccatcc ggtaataaag catacagaat tgtttcgaaa gcttcttttg
     2821 agccatccag tacatcatga gtaatcgaca catcatcaaa catatccacc acatcaccat
     2881 gagtaaaggt ctgagaaccg ttttctaggt gggcctttgt ttggatagaa acatggtgtt
     2941 ccactgtaca actgacagtt tgggcttggt tattcaagct aacatccttc gctactggct
     3001 tatcgcctgc ttcaaaagct tccttatttt catacacata gttgaataag acaatagttt
     3061 tatttaaaac ttgttcggcg gttaatttat gctgaatttt ccaagttcct gttttctctt
     3121 tgttcgccaa cgttgcgctt gtttctgcaa tcaccactgg tttggctttt tcatcttttt
     3181 cttgggcagc ttttgtcgca tccacatcaa tggcttgcgc taccacatac cagtcttctt
     3241 tgatttcatg tagattgtaa ctcaatttat caactagctc ggtagaatcc agagaagtta
     3301 attctttgtt tccgtctttc caagtcgcaa gagaagttaa actatcttct tcctcaggca
     3361 aatcatagag cattaaacgg ttcaaactaa cagaaaatgc tttgttcgtc agcttctcgt
     3421 aaggaacggt caccgtttta attggttgtt tttggtcttg ttcggtaatg gtaaagacat
     3481 actcactctt cacaaagtct tctttgtttt ccttaaaagt agaacggatt tctaatggcg
     3541 taatcttttg aaaaccttct ggggcttcta cttcttcaag taaataatcc ccataaggaa
     3601 gattttcaaa cttgccataa ccatcaaacc ctaattgctc gttataagct gtggtcgctt
     3661 catcttcggc acctgtgatt tcattggtcc cttccaatgg cgacacttta aaggtcagat
     3721 cattgaagcc agtttcggta gtgcccgcag ctgatccagc aaatttaaag aaatcaaagc
     3781 caaagcgaat cacttgttct tttgccgtaa catttcgggt aatcacggca tttttttcgc
     3841 tatcatccac ctttttgatg gatacaggat actttgtttc atcaaggcta tatccttcgg
     3901 gcgcttttgt ttcttgccaa taatactcgt tgatagctaa atgtttaacg gcaacttggt
     3961 tcttttcgtc taaagtcaaa gtgaccgttt catcagaacc tttcgttccc ttcgctaact
     4021 ctggtttaaa agcctcactc catttaacag cttttccatc cttagcagta aacaaggtgt
     4081 attcagcacc ctcaaagact gccttacctt gtgctttatc cccagtatct ttgtcttctt
     4141 tcgtcaaagt ggtttcgcca gtgacttctt gattttgccc ttttacgtta ctggtaacaa
     4201 gggctacggt ttgattggca tacttcaatt cgacttttac aggtttgaac gtattcacga
     4261 acccatgact agctttagtt tcggtcacat aataggttcc caattccaac gcattagcaa
     4321 tctcttttgg tgtttccgca tgaccgtttt catccgtggt catttcttga acgatttcac
     4381 cggttggact gtctttgcga atggcaaacg tattaccagc taaggaataa ttgtcattcc
     4441 aaagatcact ccctgtttca actccgcttt tatccagaat gatttgccct ttttcccgtg
     4501 cgttttttga agtcacgtaa atggtttcgc ctgctttaat ggtggtagtc atgggggtgg
     4561 tatcaatcgt ataaggcgcc ggtactgatt tttcggtaat ggttactttt gtaccatgag
     4621 gaatctcatc caatgtagca atgccttctt tgtcagtagt cacgtctttt gcaggtaaag
     4681 tttttccaaa gtctaaatgg aaaaccgttc ctggtacaac atcacccgat tctttatcga
     4741 cttttttgat ctttaaagaa ccctccgttt ccacatcaat tttgattgtg taggtattag
     4801 gtttatcaat cgccccggcc attaaagtct gttggccagc ctttttatag gcgacaggtg
     4861 ttccggtacc agcagatttc ttaagcgtca gcacaccact ttcttttgaa ttggcatttg
     4921 gggtgattat caactgatta ccattcacac gataatcaat gtttgcggca ttttccacaa
     4981 cctcatcgaa ctctgacaaa ttcagattat tcgtatccgt cacagtagtc gattgcccta
     5041 atactgtttt agccgtacta ttatggaagg ttggtttctt ctgataatcg tcaatggctt
     5101 gatttatttt tgcctcaatg gctgcgatat ttacggcact accatctgat cgttttattg
     5161 agtgaagggt atacccatta acttcttgcc agatcatctt ctgtgccaca atatgagtat
     5221 ctgcatctgt acctactttt ttccataaaa cggaaactaa tttggcttta tcggacatat
     5281 ctggtaacgg gtttttctca tagccaggag tgaactcatt gtaaatcggg actcctggtt
     5341 caatacagaa aatatcttgt ttaaagccgt tgtacactgc ataatgcgga acagtgactt
     5401 ctgacattgg acgaccatca ctaaaagttc cttcaacagc atattgatgt gctaaatcta
     5461 gttctacggt taccgtttgt ggatgggtaa tttcatcagc aaaagtaatc gtaggagcaa
     5521 ttgctccaac aattgcttga acagccaaac tcaaaatagt taatacacat aatttctttt
     5581 tttgtttttt atacatgaaa tgttctcctt tcaaattaag cacaaaaaaa taagatgctc
     5641 gcttgaacat ctttgtttat aaagtctttt taaaattttt attgactgaa tagaatggat
     5701 ttgagaatga atgagcctaa aaatttaatc taaaatacga aatatactgc ctttaaatta
     5761 gaacacattt tagaacacaa acatttcaaa tttaatcaaa tataataaaa tatatttacg
     5821 ggaaaacctt tataaatcaa ggtttttcaa acttattcaa tgatacgaag aaaaaaccag
     5881 gtcttaagaa agcccgtaaa gcttcacagt tctcaaaacg ttaattgcga ttatcgttcg
     5941 agttcacgag ttacgagaat cgtactgggc gaagcaatgc tgagcgcagt taccttaatc
     6001 ctaacttttc tggcactttc cacaatttgg aaagtgcctt ttttgtgttt ttagctatgt
     6061 taataggtag gattcttggt ctataatatc attcgatcta taaacaaacg ggaaaaagat
     6121 aaagtatcat caaacaatat tatttatccg cttctgaggt tgtgacagaa gtggtcagct
     6181 tcaagaaata agaaggaatt tacaaaaatt gcttctcatg tttgataaat attgtgactt
     6241 gtgaccgagg agttggcagg agaacaccgt ttattcggaa gaagggattg tgaaaagtct
     6301 tttgtcataa cctcttattt ggtagctaat tgtaaatccc aaacggcatg tattattttt
     6361 cataagtccg tttttaagga ttaatgtttt cttttgcttt ttatatggtc tggcttcagc
     6421 gagaagttcc aacgcatata caaggacgtc tgatgtcaac gataacaacg attaccatgg
     6481 tggccagtcc acttggatta gcactgtttg gtgcgctctt tgattggcag acaactcaac
     6541 cacgtctgcg agacagtctg atttttcttg aaattcaatt ggaaaattta cactttcttt
     6601 atacatttct ggctgctttc tttatacccg tcaactatac ttcacttatc aataagaaag
     6661 aaggataagt atggattttg gaaatgaaac ggtcttgaaa gcaactggta taaccaaaaa
     6721 atacggtgct gccaaagcat tagataaagt atcgatcgaa atcaaacgtg gaatgatcta
     6781 tggtttgatt ggggagaatg gagcgggtaa gtctaccttt atgagaacta ttatgggttt
     6841 gatctcgatt gatgaaggtt cgatcgaatt atttggaacg acggatttgc aggcagcaag
     6901 acgacgtatg ggtcaatcga ttgaaacccc tgctctttat ccagaattaa ctgcaagaga
     6961 taacctacgt atccaagcag cgaacggcgg tgtgtcggat agagaaatcg aagacctgct
     7021 gaaaatgatg cgattggaaa atacagggaa aaagaaagca aagaatttct ctttaggtat
     7081 gcgccagcgt ttagctattg caaatgcact gatcacgaat ccagaatttt tgattttgga
     7141 tgaaccgacg aatgggatgg atccagcagg gatggcagaa atgcgagaaa tcatccaacg
     7201 tttggtaaaa gaacggggca tcaccgtctt gctttctagc cacttgctag atgaattgtc
     7261 acagatcgcc actcactacg gtattttgca tgaaggacac ttgatcaaag aattatcgaa
     7321 agaagaattg gcacaagaat ctcgtcaatt tataaaaatt gacacttcgg cgacggaaca
     7381 agcagtaacg gttctcgact ctcttggtta tagggattat tttgtccaat ctagccgtgt
     7441 tatccagctt tttgaaggaa ttgaccaagt agcagccatc aatcaagcat tggtagaggc
     7501 aaaagttcct gtggatggta tccacctagt tgggcaaaaa ctggaagatt atttcttaca
     7561 attaactgga ggcaatccac atgtttaatc tattaactgc tgaaaaaata aaagtatgga
     7621 agagccgtaa aatgtgggtc tcactaggac tgatggtcgt acttccgctt ttttattctt
     7681 ggaacgaatg gtatatgcat acaaaatacg gcaatgaatt aaaccaagca acggatacag
     7741 tcatcaacgg tgcaacaggg attttaatgg tggaaaaaat ggctgcatgg attcttctgg
     7801 ctttcgcagc atttgcgtgc ttttatattg gggaagaatt tcaaaacggt acgattcgca
     7861 atacgttatc tttaggacgg aatcgaatga cttattatgt gtcgaaactt ctcgttacgc
     7921 ttttgatcac aacacttggc gtggttttga ttacggggct agccatgatt gcttacacac
     7981 ttgctttcgg atttggtgaa gttgagggaa tcaaaaacta tggaaattat gttctaaaag
     8041 tatttcctgt attgcttctg ctgatattag cgacattatc tgttccagtg gctttgacat
     8101 tcatcactag aagtaccagt gtcagtttgc ttctttcttt tctttatatc atgggaacgg
     8161 ccttcgttcc cggtgttttc gcaaaaatca aaggcttgga atttctaacg gagtggttta
     8221 cggaaacgtg gctcatgtat acggactttg cgcaacaagc aacttattcg caagcaccga
     8281 aaatgattct ggtcagtcta gtaacaatcg tggtatcatc agctctagga atgtttattt
     8341 tccaaaaatc tgacattaag taggtgttcc tgctggaaac tattttgatt atcgaagacg
     8401 atgaagctgt tcattcttta ctggaggagg ttcttgaaca gagatacaaa cttcttgatg
     8461 cttattcagg tacggaagga aaattattgt taagcacata tcctgtcgat ttgatccttc
     8521 tagatctcat gcttccaggg ctttcaggtg aagcattgtt agcagaaatc cgacagacat
     8581 cgaatgttcc gatcatcgtt ctttcagcaa aaagcgatca aagagataaa gtttccttgt
     8641 tggcagcagg agcggatgac tatgtgacga aaccatttga tatcgaagaa ctgttgttac
     8701 gaatcggcat ccaacttcgt catcagactt ctgtccaagt aaaagatctg tcgcaacgca
     8761 tctattacaa ggaaatctta ctggacaaag agtcgaggga tgtaaatgtg aatggtgtgg
     8821 ctgtccattt gacaggaaga gaattcgatc tgctgaagct ttttttagaa catccgaaaa
     8881 aagtattttc gcgagcaaat ctttatgaaa cagtttggca agaaccatat tttgatagtg
     8941 agaaaacagt gaatatgcat atttccaatc ttcgagggaa actagcaaag gcacatgctg
     9001 tgtatattca tacggtgtgg ggagtggggt ttagatttga ttaagtggct gttgcttctc
     9061 gttgttatct ttatgggaag ttttattttc ttcctgcaaa gggaaatcaa gagattaact
     9121 aagaaaatcg ccactctttc tgaacgacaa gaatatggtg gtcgtctttc agtcgaattt
     9181 cgagaaaaga atctaatgga cctagtagat gcactgaata catgggtgga tgaaaatgaa
     9241 caaaaaggac aagcatttca agaaatggaa gaaaacatca gactatcgat cgtcggcctt
     9301 tctcatgatc tcagaactcc tttgactgct attaacggtt atgtacaatt actggaacag
     9361 gtagaggata cggagaaacg ggagaagtat ttaacgatca tcaaacaatc tgttgcaaga
     9421 ttactcagta tgacggatca gttttatgat cttgctcgaa tagagacgaa gcaaaaagaa
     9481 atggatctcc atacgctttc tttttctaaa gaggtggaag aagtattttt ctctttttat
     9541 gaacaatttt ctgagaaaaa tatcgaggta gcgttcgctg aacactcgat aaatacaact
     9601 gtattagcag atccgctcat gttgctgcga gtgctgcaaa atatcataca gaatttattg
     9661 cgttatgcag atggacaagc aaaggtgaca ttttcaaaag aggattcatt cattcgatta
     9721 acagtggcca atcctatcaa accaggagcg caaacctctg ttgaaaaagt cttccagcgt
     9781 ttttataccg aaaataccag ccgcacaaat acggaatcta gcggaatcgg gctttacctg
     9841 tcaaaaaaat tggtggaagg aatgggagga gagatgaccg ctagattaga cgggggcatc
     9901 ttctccatct ccgtcaagtt gcggagggtt taattggctc taatttacct gaagtgaatg
     9961 acaaaaaatt gtatctcgtt ttaaagacat cttctttttt ggaaggtgtt tttattttgg
    10021 tgtgattgat acgaattagg taacgaagac agttattttt gtaccccctt ttcaaatagc
    10081 gcaacagtgc tatactaaaa ctaacaaatc aaggaacgga gagggatatg ggccaaagag
    10141 aatttatcgt tttaattatt attgcagttg ttattttatt agttgtttta gacatttttt
    10201 cacgtttgaa agtaaaggaa acagttcgta gtaattgggg aaaaattcct tatcaaccga
    10261 gatttgataa agaagaaagt ttgaaggatg cgtggctcac ggagaaaaaa ttccgttcat
    10321 gggatagtga aatcgacgat cttacttggt atgacttgga tatgttcgaa gtatttgaag
    10381 ggatcaacag tacctattct agtgtaggtt cagaagcgtt gtatcagcgt ttacgtagct
    10441 ttgattttgg agaagattat cagttagaaa aactaattgc tttttatcag gaaaatcctc
    10501 aactgagaga aagaatccaa tatcaattcg ctcgtttagg aaaaaaagat cataattttg
    10561 caaaacaata tctagcggat ggaaaaagta aaaagattgg acgcgtaggt ttatttattt
    10621 ttcttggctt gcttccgctg gtgggtgtag gtttgcttct tttagggcaa gtactaggaa
    10681 tctatctagc aatcggtggc ttagtattca atacggtcta ttatctagtg aagaaacaaa
    10741 cactagaaac agagctgaat agcatgagtt atttggtttc aacgatttca gccgccaatc
    10801 agttagcaaa aatgacgaca ccacttcaaa aagagctgaa acagcaaact aaaccattaa
    10861 gagacatccc taaattaggt ttttcatttc gaatgaagag cgggagtgaa gcagaattga
    10921 tttttgaata tgtcaacatc atgttcatgc tgccatttat ctcctatcat ttcgttctca
    10981 ctaaattaga aaagcattca actgaagcca tcaagctgtg ggaattgctg ggagaattgg
    11041 aagttgcagc agccgttttg aattatcgca cctatatgcc gatcacttgc cgaccagagt
    11101 ttactgaggg cggtgtgaca gcgaaagata tctatcatcc attgttaaaa gaagcggtag
    11161 ttaatccagt caactggcag aagaatacat tagtcacggg ttccaatgca tcaggaaaat
    11221 ctacgtatgt caaaagtata gccatcagct gtattctagc acagacgatc caaacagcga
    11281 ttgcagaaca gtttaccctg caacctggac atgtgttgac ttccatggca atcgaagatg
    11341 atttatttga aggcgacagt tattttgttt cagaaatcaa atcgatcaaa cgtttgctgg
    11401 accaagtggc ttccaaagaa cgctgctact gcttcgtaga tgagatttta aaaggaacaa
    11461 atacgatcga acggattgct tcttccgctt cagtcgttcg gtggcttgcg gagtatccat
    11521 ctttagcatt tgtcgccaca catgatatcg aattgacaga gattttaaag aattattgtg
    11581 agaatgtcca ctttgaagaa caagtaacag aaggaaaagg aatcagtttt gatttcaaat
    11641 taaaagaagg accttcaagg acccgaaatg cgattgcctt gctgaaagtt ttgaattatc
    11701 ctaaatccat agttgaacag gcccaaaaag aaagtttgtt gttcgatgaa cagcgtcagt
    11761 ggtatccgtt tgattaactt tatcaaacga agcatgatgc gtgggtccaa aaagacgatt
    11821 atcaaaagaa gtacattgat gccaaaaaag tggcagaatt caatgggaac gagtctgctg
    11881 tccagatgat cttggaagat ttagcaaaag acgatacaaa ataaaccacg ctgtcttcct
    11941 tctagcagct tttatcgaaa aaacttagca tcagcgacga gacgcacgtt ccatggcaaa
    12001 gttctccgta aaacaaacag cgaaaacaga aattgaaaat aactatttcc attttattta
    12061 tttcggttaa aataaaagaa aaaagctttc gataaaagga cggaggaaag cttaacatga
    12121 ataaaaaaga caagaacctc agttataagg atcttctata tgtcatcgta gtagttgctg
    12181 tcggtacttt tatgatttta aaagttccag agttgtttag atagttattc tatcgaagat
    12241 gatcatgaac tagaaaacac atctcctctt tgttagcagg tgtgtttttg tgtggaagcc
    12301 aagttgtttt ttcatctgct gtattccatg atacaattaa gaatcatgtc tataaggaga
    12361 taggaaataa atggcaaatg cgcttgaaat aaataatttg aaaaagatct atggaagtgg
    12421 ggtcgaagca ttgcggggaa tcgatctttc ggtgaaagaa ggagatttct atgcgctatt
    12481 agggccaaat ggagctggga agtctacaac gatcgggatt attacttctt tagtcaataa
    12541 aacatctgga aaagtgaatg tcttcggtta tgatctggac aaggatctag ttcgagcaaa
    12601 gcaacagatt ggattggttc cacaagagtt caattttaac ccttttgaaa cggtccagca
    12661 aatcgtcgtg aatcaagcag gctactacgg tgtctctcgc aaagaagcga tgaaacgcag
    12721 cgaaaaatat ttgaagcagt ccaatctttg ggaaaagaga aatgtccgtg cacgtatgct
    12781 ttccggggga atgaagcgaa gactgatgat tgctagggca ttgatgcatg aaccacgttt
    12841 gctgatcttg gatgaaccaa cagcaggagt tgacatcgag ttgagacgcg agatgtggga
    12901 gtttctacga gaattgaacg aaaatggtac aacgatcatt ttgacgacgc attatctaga
    12961 agaagctgaa atgctttgtc gcaacatcgg aatcatccaa tcgggtgaac tgatcgagaa
    13021 tacaagtatg aaagagcttc tgtcaaaact gcaatttgaa acatttatct ttgatctagc
    13081 gccatatgat accaaacctg tgattgaagg atatgcttgt acatttgaag atgagcagac
    13141 aatcgctgtc gaagttgaac gaaatcaagg ggtgaacgga atcttcgatc agcttacaag
    13201 acaaggaatc aaagttcttt ctatgaggaa taagtcgaat cgtttagaag aactgttctt
    13261 gaaaatcaca gaagaaaata atcatacgga ggagaaaaat gtttaatctt tattttactg
    13321 cattaaaaag tttagctatc aaggaaacga accgctattt gcgtatatgg gtccaaacac
    13381 tggttccgcc agtgatcacg acatccttgt attttgttat tttcggaaaa atgatcggtg
    13441 ggcggatcgg tgacatgggt ggtttttcat atatggaatt catcgttcct ggtttgatta
    13501 tgatgtctgc tatcactagt tcgtatgcca atgtttcttc gtccttcttc tctcaaaaat
    13561 ttcagaaaaa tatcgaagaa atcttggtag cacctgttcc tacgcatgtg attatttggg
    13621 gctttgtgat tggtggtgtg ggtcgcagta ttctcgtcgg ctctctcgta acgatcattt
    13681 ccttgttctt tgtaccactt catgtgtatt catggttcat ggtcatcatt acattattga
    13741 tgacggcaat cttgttttca ttagcaggac tgctcaacgg gatttttgct cagtcttatg
    13801 atgatgtatc gatcgtgcct acatttgtct tgcagccgtt aacttatcta ggtggcgttt
    13861 tctatgccat ctctatgtta ccgccctttt ggcaagctgt ttcaaaagtc aatccgatcg
    13921 tttatatgat ctccggtttc cgttacggat tccttggaac gatcgatgtg ccagtagcaa
    13981 catcgatgat cgtacttgtg ttattcatcg ttgttttgta cgccgttact tggtatttga
    14041 tcaataaagg aagaggattg agaagttaga aaagaggttg tgaaatcagc gttttgacct
    14101 gagtattaga gaaacaaacg ggaaaatagt tcctcatatt ttgttgtttt ttgagcgaat
    14161 accgagggtc agctgattga acaccgttta gtgga
//
LOCUS       DACEMK010000069        14094 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEMK010000069 DACEMK010000000
VERSION     DACEMK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.071"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..319)
                     /locus_tag="IW715_001881"
     CDS             complement(26..319)
                     /locus_tag="IW715_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1022486.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="IW715_001882"
     CDS             complement(347..1012)
                     /locus_tag="IW715_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1022487.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="IW715_001883"
     CDS             complement(1152..1784)
                     /locus_tag="IW715_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1022488.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /locus_tag="IW715_001884"
     CDS             complement(1791..2858)
                     /locus_tag="IW715_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1022489.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /locus_tag="IW715_001885"
     CDS             complement(2855..3586)
                     /locus_tag="IW715_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1022490.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IW715_001886"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="IW715_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1022491.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IW715_001887"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="IW715_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1022492.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="IW715_001888"
     CDS             5709..6869
                     /locus_tag="IW715_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1022493.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6909..7856)
                     /locus_tag="IW715_001889"
     CDS             complement(6909..7856)
                     /locus_tag="IW715_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1022494.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7989..9380)
                     /locus_tag="IW715_001890"
     CDS             complement(7989..9380)
                     /locus_tag="IW715_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ1022495.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            9506..9916
                     /gene="tagD"
                     /locus_tag="IW715_001891"
     CDS             9506..9916
                     /gene="tagD"
                     /locus_tag="IW715_001891"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ1022496.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            10096..11385
                     /locus_tag="IW715_001892"
                     /pseudo
     CDS             10096..11385
                     /locus_tag="IW715_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11452..12879)
                     /locus_tag="IW715_001893"
     CDS             complement(11452..12879)
                     /locus_tag="IW715_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1022497.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(12876..14018)
                     /locus_tag="IW715_001894"
     CDS             complement(12876..14018)
                     /locus_tag="IW715_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ1022498.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
BASE COUNT         4606 a         2698 c         2083 g         4707 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 tttttgtttc aatcgatttc tgtctttaaa taacgttcca ttccgttcaa tatatcaaat
     6961 ttgggttgat aacctaaaga acggatcttt gaaatatccg ctaaagaatc acgaatatcc
     7021 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
     7081 tgaatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
     7141 gctttttctt ccttagcaac aagtaaaagt gcttgtataa catcttctat ataaacgaag
     7201 tctctagatt ggctaccatc accaaataaa gtaaattctg ttttttctcc tgccaattgt
     7261 tttttgtatc ggtccactag aatcgaaata actccgctat atggagagtt aggattttga
     7321 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
     7381 taatccaaaa catattgttc tgatgcaaac ttatcgatgg catatggtgt taaaggacgg
     7441 atgaccgatt cttctttttt tggcaacgtt ggttcatcgc cataaactgc agctgatgaa
     7501 ctaaatacca atcgttttaa accagattga tattttcgta ccaattcaag caataaaagc
     7561 gcactgtcaa aatttacacg atgagtttct agaggacgtt ctacagaatc tgccacacta
     7621 gctacagcag ctaaatgaaa aatataggca aactgatact gtttcatgat tctttccatc
     7681 aattgaggat ctgatacatc cccctcaatg aaagtcactt gtttctccat atccaagttt
     7741 tctttttttc ccattgataa atcgtcgact actacgattt tgttttcttt tcccaaataa
     7801 ttcgctagcg ttgaaccgat aaaaccagct ccaccagtaa tcaatatagt atccatcaaa
     7861 ttcactccaa ttatgcattc tcatacttaa taaagttagt ttaccatttt tatattgtaa
     7921 aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt atatacctaa
     7981 aaatagtttc aattagttac tccttcttct ttagatatag ttttttggat agaacccaga
     8041 tatccccaaa aaataaatga acctatcgta ctaaccaaaa tgagttctgt gataaataat
     8101 gccgaaactg ctattgtaag aatcattaaa atagcaaaaa agtgcttatc ttttactaga
     8161 tttggttgtg tgaaacaata acgcaaagtt ttaaagcatt ggataaataa gaaggttaac
     8221 aatgtcaata gacctaatcc tcctgtagca gccataacta agaaaataaa attatgagca
     8281 gttaaagtat cttttcccat taatgtatat ggtagtttat catgtgcgta ttcaatatag
     8341 tatcctggag aaccgccgga tatcggctta ctcttgaata tttcaattgc acttttccaa
     8401 agtccaaacc ttaaattaga aacatccccg ccatcagtgt cagggcgttt taaatcaact
     8461 ttattgctgc tagttgttcc tgatgaatca gatatatctt tttttatttc tgtaatatgc
     8521 ggaacatgtg cccatgcttg agcagttata tctgttacat aatacatcgc aattatactt
     8581 agcaatccta ctactaatgc agttagccat ctttttaaag tattagtttt aagaaaacga
     8641 ctactataac ttgtaaagaa actaaaaaca aaagttgcta ttaataaaca aactaatgtg
     8701 gtgcgtgagc ccgataaagc aataaacgag aattgaataa aaatattaat tgcaaaaaat
     8761 attttaaata gaagatttaa atgcttgtaa gtgaccaaat aatatatagc aataatgatg
     8821 acaattaatg atataaccgc accaaaattt ggatctgtat atacaccaaa taaacggttg
     8881 cctaaccacc ctaaacgaac tgggtaatac ttatatttta ttggaatttt tatgccatat
     8941 tgaatgacaa accagtataa tgatactaaa ttagctaaaa accataccaa tgaaagtatc
     9001 caagtaaata ctttgaaaaa aaagttacta ctgtagtctt tgccaaactg ataaatcata
     9061 aagaaaaaga ttaattgcca catgattaac ttgatatttt cgctatatcc atattttaaa
     9121 ttcattaaac tgcttactag catcgcgcca ataaaagcaa ttaatagcca atcatatttt
     9181 ttaagatttt tttctctaaa tgcaataatt gcatctataa tgatcaagac aactcccgct
     9241 attccagcag gccaaaaaag taaagtatca tatctagtag gtaatagtaa atacgggcct
     9301 attgctcgta acattagaat aaaaccaaaa taaataaaat accaaccttt cacatatttt
     9361 aaaaattttt cattcaacat ttttcctcca tccacctatt tctcataaac atttcttatc
     9421 ctttttagga taaactggtt ctttcaattc taacaaaaaa atgctaaact aacaatgtat
     9481 attaatgaat ttcgagaggt gaaagatgaa aagagtaatt acttacggta cctttgattt
     9541 gcttcactac gggcatatca atttactgaa acgagcaaaa caatatggag attatcttat
     9601 tgttgccctt tctacagatg aattcaattg gaatgcgaaa caaaaaaaat gttattttag
     9661 ttatgaaaaa agaaaacagt tacttgaagc tattcgctac gttgatttag ttattcctga
     9721 agaaaattgg gaacaaaaag taaaagatat aaaagaatac catgcagatt atttcgtgat
     9781 gggcgatgat tgggctggac aatttgatga acttgaacaa ctaacagatg cacaagtaat
     9841 ttatttacca agaacacctg agatctctac aactcaaata aagaacgaac ttaattcttt
     9901 aaatagtgat aaataatatt ttttaaacaa tgaaagaaca atatatctgg ataagacgta
     9961 aaaaaatggc tctataattt ctaggactga tagaattttc tgtcgatcct agattttctt
    10021 gtaaaaaaaa cagacataca aaaatttcga taaaatagag tcgtctaaaa catcaatctg
    10081 aaaggaagat tttgtatatc catttctcgt tctatcaaag agcacttcac tattaaagat
    10141 aaaaatattg aaattaattc ttttcaaaaa actttttata aggacaagaa agccatcgta
    10201 atcgaaggta cattagttcg cacttttaga cattgtctat tttgtaaaga aactgcgaag
    10261 cagctcgtga aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa
    10321 taaacctatt tgcgtcttag aaaacaacat taccactacc aatcatgcgg aaaatatttt
    10381 actgctcata cgtatcttgt cgttccttca tgttttattt cgaaacaagt tcattacacg
    10441 atattagaag agttgaccga gcgtcaagcg ttaaagacac ttacgaaacg ttgtgatctt
    10501 tcagtaacga catttcaacg gacattatct gctctatcgg caatgctgcg acagatgcta
    10561 gactatttac ctcattgttt actattcgac gagtttcgct ctctttcaat ttcccatgga
    10621 aagttctcct tttcttgtat gaatagagag acagggaagc tctttgacat tttgtccagc
    10681 cggagaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg
    10741 gttcgataca ttgtcacaaa tatgaatgcc ccttattttt cattagtcaa agagtccttt
    10801 ccaaacgccc aaatcatcat tgattgcttt cacattggac aacacttgaa caagtttttc
    10861 gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aagacacttc acaagctaag
    10921 tactatcgac aattgaaacc tttgtataaa ttacttctaa aatcagatga cgagttggat
    10981 tacacaacat tcaagaaatg gcagaatttt caatggcgct atttaacaga aagtgaagta
    11041 gttgatcgtc taccctcaat ctcttatgaa cttaaaattg ctaagcagtt tatcaaaagc
    11101 tgcttagcaa ttatcacaag aaacagctag ctacttcgag aaacgaccaa agaagttccc
    11161 tcgaaacttc actatctcaa aaagcctttt tcatatctga aaaaggcttt taactagcat
    11221 tttcgaaaaa gtattctaat aacaaaatta gagaatcttc gcacacatat caaaacattg
    11281 aaacgatttg cttatgggtt tcgcagtttt accaatatgc gcttgtggat ttttctaatc
    11341 aacaggttaa tcagctatgc attaaaagct agtaactctt actaactttg ttgttcataa
    11401 atgaccttat cagcccgatt tgacaaagaa ctactttttt acaacattat tttattttcg
    11461 agtaatcttt cttatttcat ttcgtatatc tggaaaaaga aaataaacaa acggaagata
    11521 aataatgaaa atcattattg atttaattgc catatctaaa aatgaagaag agaataattg
    11581 tggaagaaag taataggccc aagtagtcaa agtaagattg actataaaaa ataagataac
    11641 gttcttccca aagaaatagg atatttttat ttcttttcgt acaaaaaata ctctaaagaa
    11701 aaagactaat aattctacta ccacgagtac gatcgtagcc gctacagcgc cataagtagg
    11761 cacaaaagct acatttccaa taatacttac aatcgcacca atgatcattg gataagcata
    11821 ctccttatct ttttctatag caattgcaaa ttggttagca aaaactccac caattggact
    11881 aacaactata attagacaag aaataagcat gtttattgtc atcggtttga aattctcccc
    11941 aaaaaaccat ggaacaaatt cttttgtatt gataaacacc acactaaaaa gaattaatgc
    12001 aatgataatc gtgtaatcaa aagatttttt tgtgtaaact aaaatatttt ctgatttccc
    12061 ctctgcctgt gctttagata ttttaggcaa cataacaata gtaattgatg ttaagactgc
    12121 caatataata cgagcaattt tttgagactg atcataataa gataattccg ttggatccac
    12181 aaaataccca actatcgttt tgtctaaagt tgtgtacact tgcataaaca actgaggagt
    12241 caaaataata aaagctggtt tccacaactg tttaaaaaca ttagagttaa agattggttt
    12301 acctactttt ccgtggatac ttaaccagaa aactaaactt cctaaaaagt tagtaattga
    12361 agtaatgaac acgtaaatcc acaaatcatc tttagttgaa acaaaaatga ttattaacaa
    12421 taccgatgat aatttgataa ctgtatttct aataaccact ttcgaaaatt cttcaattcc
    12481 ttgaaaaaac caagaaatat ctaatgttag ccctacaata aatatcaatt gaattaagta
    12541 taaattttta tactttacat caaaaaaata aatataggct atatagagaa aggaaacact
    12601 caagcctatt aaaaattgaa taaagtaaat attccagaat tgtaaagata gtgctttttt
    12661 atctttcctt gatttagcga tatttcgaat acgaaaagga ttcattccca cagcaatgac
    12721 agtcattaaa aactgtgata ttgagaaaac atacccataa atccctaaac cagttgaacc
    12781 aattctacga ctaagtatag gtatagtaag taagggtaaa acaatcaaaa agacttgata
    12841 tatcgcatta taaaatatat cttttactgt ttttttcact ttatatccct ctctatcaac
    12901 tcaagaacct tttctgtact tgctcctgtt tcataatttc caatataatc aagtaactct
    12961 tgagaacttt tcaaaagctt ttcttgttgg tttttaaaaa catcaacaag gccataacta
    13021 tcttgtacgc ttataattgg taactcactt aactgtatat aaaaacctct ttctaagcta
    13081 tattcttttt catcatagtt aaaaagaata attggttttt ttgttagcgc aaaatcgaaa
    13141 aaaatagatg aataatctgt gattaaaaca tctatctctt caaaaagcat ttcaagagga
    13201 atcccatcat taattaaagt aatttgatgt tttttcatat attctatact tttagaatca
    13261 gttgctaatt tactatgaac attaggatga aatttagtca taaaatttaa ttcttgttgt
    13321 tgctttttaa aaaattcacg taagttatcc acacctaacc attctagttg cgtaattccg
    13381 ttgtcctcaa tattatttcg aaaagttgga gcaaataaga cattgatttg tttctttttc
    13441 aagtcaaaca cttgctcttt actagaggct aattcatcgt ttctaggagt tcctacttct
    13501 aatattttag aaggaacgat attgaatgct gaaacatact tcttagaact atagcgatta
    13561 ccagataata aatatgtaat tgactcacta tcttttttcg catagcttag gtaacgttta
    13621 ggtaacttat cttttgcatc aaattcaatt ttttttaaag gcgttccgtg ccatgtttga
    13681 aaataaagtt gttttttcct ttttgcatac ttaatcgttt tccgactatc gtctatccaa
    13741 tacttagctg tatataaata aaaggcagct aaaaaagaat cggagtcaat tcttttaata
    13801 taatctggta aatcagttat aatcgcttct tttttgatta accaaataat tctgtaattc
    13861 aatttttttt catgaatttt ctctgaaata actcttggat ttccagaata gttacgcccg
    13921 ccaaaacttg aaaatacaat taaatttttt tgaataggta atattttaat aaaaaccggg
    13981 aaaatattcc ttgaaaaaaa tttttttata ttcttcatct cttacctcac agattctatc
    14041 ttaatcaaga atttacttgc tatcttacta cttgacatat agtaaggcaa gttt
//
LOCUS       DACEMK010000070        14018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEMK010000070 DACEMK010000000
VERSION     DACEMK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.072"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            134..454
                     /locus_tag="IW715_001895"
     CDS             134..454
                     /locus_tag="IW715_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022499.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            906..1106
                     /locus_tag="IW715_001896"
     CDS             906..1106
                     /locus_tag="IW715_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1022500.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1253..4042
                     /locus_tag="IW715_001897"
     CDS             1253..4042
                     /locus_tag="IW715_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1022501.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4089..4616
                     /locus_tag="IW715_001898"
     CDS             4089..4616
                     /locus_tag="IW715_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1022502.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4637..5827
                     /locus_tag="IW715_001899"
     CDS             4637..5827
                     /locus_tag="IW715_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1022503.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5867..7165
                     /gene="asnS"
                     /locus_tag="IW715_001900"
     CDS             5867..7165
                     /gene="asnS"
                     /locus_tag="IW715_001900"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1022504.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8218..8892
                     /locus_tag="IW715_001901"
     CDS             8218..8892
                     /locus_tag="IW715_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1022505.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8889..9230
                     /locus_tag="IW715_001902"
     CDS             8889..9230
                     /locus_tag="IW715_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022506.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9260..9619
                     /locus_tag="IW715_001903"
     CDS             9260..9619
                     /locus_tag="IW715_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1022507.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10143..12224
                     /gene="cadA"
                     /locus_tag="IW715_001904"
     CDS             10143..12224
                     /gene="cadA"
                     /locus_tag="IW715_001904"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1022508.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12535..14001
                     /locus_tag="IW715_001905"
     CDS             12535..14001
                     /locus_tag="IW715_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1022509.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4643 a         2428 c         2997 g         3950 t
ORIGIN      
        1 aggttgtgac gtggtcacga cctctttttg acgattatga tacaaaaaac atcatttatg
       61 aacaaaatgt gaataacggg cgttcttgag atatgatttt aattgggaaa aacttatcga
      121 taaggaggtc tttatgaaaa aaagactgtt atggtgtttg ctgtttctat ccctaagttt
      181 aacaagtcag ccagtttttg ctgaagaaac acagcggtct cctattacag gcacacgtct
      241 gtacaaaaat gtttatgtac agacaacaga tcttgattct gcgattcctg aaacagaaga
      301 acggtctttg cagccaagat ttggttactt ttggtactgt ccgacctgtg attatgaaag
      361 ttgctggcat ttcttacatt ctacagctgc agcttttgct tttactcata ctcagaaata
      421 tgatggacat catcctgtta tttatgaggt atagatatga gcaaaaaaga aaaactaatc
      481 tcgttgatcg tattggtttg ccttgttctt ctggtaactg tcataggcta tcagaaatac
      541 catgagaaca tcagacctac taaagaatct tccccagcct cgaccgttga aacagaaccg
      601 ataagtagtc agaaagaata atttttttag tttaaagaag aacatcagag caaaaaaaca
      661 ccaactgaac aatatgtcgt ttattttaaa aaaagttcgg tttttcactt tatgtaatcg
      721 ctgagaaaac tttttttaat aaaaaaagtc gaaaaactct agcttttttg taaacggtat
      781 gatatactat aggagtagtt tttgttcggt aatctgtggt agcttgtttc aaataaacat
      841 cttccagata tacaccattc aagtaattgc aaaatatgtg ttgatacaca aggaggattt
      901 ttattatgga aacaggtaca gtaaaatggt ttaactcaga aaaaggattt ggattcatca
      961 ctgcagaaaa cggaaacgat gtattcgttc atttctcagc tatccaaggc gacggattca
     1021 aaactttaga agaaggccaa gcagtaactt tcgatgttga agaaggccaa cgcggacctc
     1081 aagcaacaaa cgttactaaa gcttaattaa cctatactaa aaaataaacg cccagacatt
     1141 tgcgtgtctg ggcgtttttg tctattttct ttaaaaagcg atgagagtat ttttctgcac
     1201 tttgtagtat aatttcaaaa gaacattaga ggcaggagaa gaggaagata ttttgaaacc
     1261 aagaacttat gcagtcgttg atatcgaaac gactgggacg aatccaaaag aagatcggat
     1321 catccaattt ggctgcgtca tgattgaatc aggtcggatc acttcacgtt tttcgatcga
     1381 tattcatcca gggcggaaga tttcaaaaca aatccagcat ttaacaggga tcacgaatca
     1441 acgcgtccaa aaagcgcctt atttcgaaga tgtcgcccaa acgatttaca atcttttggc
     1501 agacaccata tttgtcgcgc ataatattta ttttgattat aattttttga accatgaact
     1561 catgcgatgc ggacttccta gtttgaagat accaggcatc gatacagtag aacttgcaca
     1621 aattttcttg ccgacggaac caagctttcg cttagctgat ttgtcagaaa gccttggatt
     1681 tattcatgag aatcctcatc aggcagatag tgatgccgaa gtgacgggtc aacttctttt
     1741 gctgatcgaa gaacggatga gaaaattgcc aatcatcacg ttagagcaga ttgcgcgatt
     1801 aagtagacac acaggtatgg atacgagtcg tttcatttat catgtgattg aagaaatgaa
     1861 agaaaaacca gagccacttg atccctcatt agaaatcgtc gatgggctag ctttgcagaa
     1921 aaaagaagtg gaactattca catccgtcca ttatggcgag cggacatatc caagaaaaaa
     1981 acaagcaaaa gaaaaaatgt ttggtaaaac actgatgtat cgaaaagaac agaacagact
     2041 gatgaatgcg gtctacgacc atttcacaaa agatgaatca aaagatttga tgatcgaagc
     2101 agctacagga atgggtaaaa caatcggtta tctgctgcca ttgagttatt tggctacacc
     2161 tgaaaaaccc gctgtcatca gtaccgtttc gctagttttg cagcagcaga tcatagaaaa
     2221 agacattcca ttgctgaatc aattgctaga tcagcccata caacctgtaa ttatcaaaag
     2281 ctatcgccac tatattgatt tacagcgttt taaaggcaca cttgtggaac cgccagaaca
     2341 aaaacaatat gctttgtatc aaatggcggt cttagtttgg ctgacgcaaa cgaagaccgg
     2401 tgatttggat gaattacatt taacaaattt gaaccattca ttctttgcag atatcgcgca
     2461 cagaggtatt ggatttttag caagaaatca gtcattttac gaacaagact ttgttcgttt
     2521 cttgcaaaag aaaatcaggc aaagtaattt tttgattgtc aatcatgcgt ttttaataca
     2581 agaaacacag cgaaaagaac cattgcttcc agctagtcca tttttgttga tcgatgaagt
     2641 acatcatttg ccggaaatcg ctgaaaaagt aaatgacaaa cgattaagta ttaactattt
     2701 ccataaacaa atccagcaat tagaagaacc aaatcagctg tttgaccgaa tacaaagcta
     2761 tttaccgaaa gaacatgaag ccttacgctg gttatcgatt tatcgagaag aactttctgc
     2821 gctatgcgaa gcacagattt gtatggcaga aggattaatg agtttagtga gcggcaagca
     2881 acctaacgga cagtatccag aagaagtcat gatgacaaaa gagatgttgg atcagctgcc
     2941 actggaatct gggacagcca ttgatcacct gcagcttttt tatcaggaga tccagcagct
     3001 acagtcacaa atgcgtctga cttttgagat tatccaagaa agctggacaa accagatgcg
     3061 gatcgattta gcaagtttat atagcttatt tgatcaatta gacaagcagt acttgctgat
     3121 ggatcaatgg ctggaagact ggcgagatca tttgatccat tggttcgttc ctgggaacac
     3181 agcgcagcag atgatttttc atctccatga ttttcaagcg gctatgttgc cgtcgactgt
     3241 ttggtatcct cgatatgatc gtattcttta tattggagga acgctgaaga tcggccctaa
     3301 tcgacactat ctctctaagc ggcttggcat tgaagcagca cctcttaaag tgattgcctc
     3361 tccttatgat tatgagaaac aggcaaggat ctatgtgcca aaagaggcac ctgccgtcca
     3421 tcaagtcagc aatcaagcat atatcgatta tttagagaca gcacttacag gtctgatcga
     3481 acaagaagat cggccaatac ttgtgttatt cacttctcat gatgttttgc agaaggtcta
     3541 tcagaaaatg catctgtcta tgctagaaaa aggacgagaa atactagctc aagggtttgg
     3601 cggaagccgg gagaagcttt tgaaacgctt cattttgtca gaaaaaagcg tattgtttgg
     3661 cgctgacagc ttttgggaag gtgtagattt gcctggggat gctttgcaga tcgttgtagt
     3721 cacacgttta cccttcgata atccgcttcg cccgatggtc aaagctagga acgcttattt
     3781 agaatcagag gggatcaatc ctttttacca ggaatccgta cctaaagcag ctttgaagtt
     3841 acgccaagca ttaggtcgct tgataaggac ggagacagat cgtggcgtat tgcttatttt
     3901 ggatcgaaga ctagtcactg caaaatatgg tgcacggatc atccatgcat taccgaaaaa
     3961 agtaccgatc aaagagctag ctatggagga aatctatcag gacatccatg aattcttaaa
     4021 gaaaaatgaa gaaggaagtt agaggagctg tcttttttca taattaaaat ctgctataat
     4081 gaaaaggaat gaagataaag gagcgacgac ccttgaagaa aaaacagaca aaggaaaaaa
     4141 cgattaatcg cgtgttactt ggcttgatcg tatttttatt agcggtcatc gtgatcagct
     4201 cgatttttta cttgcgttcc agtcgtccgg cggtgcaggc gaagaaagaa gcagtagaaa
     4261 tcgcaaagaa atatgcaaat cttgaggaag ccgatcattt ttattggttt actcgaaaga
     4321 agacatactt ttcactgaca ggtacaaatg ataaaggaaa agaaatcgcc gttatcattc
     4381 ctaaatctgg tgaaaaagta aaagtattgg atcaaacaaa gggactgact gaaaacgaag
     4441 caaaacagac aattgctgcg gcccatcctg aaatccaaat cgaaaaagct gctttaggga
     4501 tgtatgatga tcagcctgtg tgggaagtag taggaaaaga aacaaatggg aaactgaatt
     4561 attacctgat tggctttgaa tctggagatg aagtcaagac aatcaaggga atttaaaagg
     4621 taaaggaatg aatgggatgg agttatcaaa aagaacaaaa cgattagaac cgtctgtcac
     4681 tttggcagct gcggcaaagg ccaaggcatt aaaagcaaaa ggcagagatg tgctgagttt
     4741 gactgtggga gaaccggatt ttgcgacacc agaaaacatc caagaagcag caatcgaagc
     4801 aatccgcaac gggaaagcta gctattatac accaacagca ggaatcccag agcttcgaca
     4861 ggcaatcgtt gattatctga agaaaaacta tggacttgtc tatgaacctt cacaaacagt
     4921 cgtaacagac ggagcgaaat ttgctttgta tacccttttc caaacgatcc ttgacccgca
     4981 agatgaagtc atcattcctg tgccttactg ggtcagttac ggtgagcaag taaaactggc
     5041 agaaggactt cctgtctttg tgaaagggga agaatccaat agctggaaag ttactgttga
     5101 acagctagaa caagcacgtt cagaaaaaac aaaagccgta atcatcaatt ctccatcgaa
     5161 tccgactgga atgatttata gcaagaatga actgcaggca atcggtgaat gggcagtaaa
     5221 aaatgatatt ttgatagttg ctgatgatat ttatggtcgt ttagtttata atggcaatga
     5281 gtttactcca attgcaacga tttcagaagc aatcaaaaat cagactatca tcatcaacgg
     5341 cgtgtctaaa acatatgcga tgacaggctg gcgcattggt tatgcggtgg gaaatcctgt
     5401 gatcatcaat ggaatgatcg ctgttgcttc ccagtcaacc agtaatccga cagcagtcag
     5461 tcaatatgca gctgttgaag cattgaaagg cgagcaagat acagtggaag aaatgcggaa
     5521 cgcctttgaa gaacgcctga atacgttata ttcgcttgtg gcagaactgc cgggtgtttc
     5581 tcttgaaaag ccacaaggag ctttttatct ttttcctaat gtaaaagaaa cgctacgcat
     5641 gtgtaaatat gaaaatgtca cacaatgggt agaagatctt ttagaagaaa caggggtagc
     5701 gcttgtgact ggcgaaggct ttggggcacc agaaaatgtc cgcatgagtt atgctactga
     5761 tcgcgctacg ttagaagaag cggtacgcag aatcaagcaa tttattgaga gtaaaagtca
     5821 aaattaatcc taaagaaatt caaggaatat aaaaggagag tcatacgtgg aacaaattca
     5881 aatcatcgat tcgaaaaacc atgtgggaga aacagtaaca atcggcgctt gggtcgcaaa
     5941 taagcgatca agcggaaaaa tcgcattttt acaattaaga gacggaacag cctttttcca
     6001 aggtgtagtt gtaaaaagcg aagtgtcgga agaagttttc caaacagcta aaaatctaca
     6061 ccaagaaaca tccgtgttag taactggaga aatccgtgaa gacagtcgtt caaaatttgg
     6121 ttatgaaatc ggaattacaa atgtggaagt ggtaagcgag agccacgatt atccgatcac
     6181 accaaaagaa cacggaacag acttcttgat ggatcatcgt catttatggc tgcgttcttc
     6241 aagacaacat gcgattatgc agatcagaaa tgaaatcatt cgtgcaacgt atgaattctt
     6301 taatcaacat cattttgtca aaattgatcc gccgatcttg actggttctg caccagaagg
     6361 cactacggat ctattcgaaa caaattactt tgatcaaaaa gcttatttat ctcaaagtgg
     6421 ccaattatac atggaagctg ctgctatggc tttcggaaaa gtcttttcat tcggtccgac
     6481 tttccgtgct gaaaaatcaa aaacacgacg tcatttgatc gaattctgga tgatcgaacc
     6541 agaaatggct ttcactcatc aagaagaaag tttagaagta caggaacaat atgtagcatt
     6601 cttagtacaa aatgttttag atcattgcga atatgcatta gatgtactag gacgagacaa
     6661 agaagtctta aaaacttata caaaattgcc attccctcgt attacttatg atgatgcgat
     6721 cgaattactg aagaaaaatg gatttgatga catcgaatgg ggcgaagact ttggttcacc
     6781 tcatgaaacg ttcattgcga attcattcga acagcctgtc tttatcctaa actatccaaa
     6841 agccatcaag ccattctaca tgaaaccaca tccaacaaga gaagacgtgg tgatctgtgc
     6901 agatatgatc gcaccagaag gttacggtga aattattggc ggaagcgaac gggcaacaga
     6961 ctatgatttc ttgctagaac aaatccgtaa tcacggtttg gatgaaaaag aatattcatg
     7021 gtatcttgat ctacgtaaat acggttctgt tccgcactct ggttttggtc taggtcttga
     7081 acgaacagtc acttggattg ctggtatcga ccatgtacgt gaagctattc cattcccacg
     7141 tttgttgaac cgaatttatc cataagaggt gatgaaaaag ctgttctgca tcaagtaatt
     7201 agaacagcca agcaaaaaca gctgttcatg tttttgaatt ggcgggactt attaccgcag
     7261 atgtaagctt ttgaataccg tttagtggag aggttgtgaa aaagctgttt agtttcgagt
     7321 attaagaaaa attttgtgga aatggctttc cacatttttg cataaatttg gcttaatacc
     7381 gagaaactgg cttttgaaca ccgtttaagg aagaggttgt gcgcctgccg ttcagctccg
     7441 agtcacaagg agtaatttca aaaaacagtt tttcatgttt gatgactatt gtgacttgtg
     7501 accgaggagt tggccggaga gcaccgttta ttctaaaaaa gaactgcgaa ctctcgcagt
     7561 tcttttttta aaatacggga taaaggaagt ttccagcaga caagagaaga aagcaaaaga
     7621 aattgctatt tgattcacaa ggttctataa cgaaatcgca atcaatcaaa catttacaaa
     7681 gaaggtaatt caaatggaaa tctgatttga tgatgcttct gctgttgaaa taaagcagca
     7741 tctagcacct gaaaacaaac taatagtaac attcgaagat ggagtaggtc cgtattttca
     7801 gcatgcaata atccatatgt aagttcaatt cacccttgaa atcatcggac cagataaagt
     7861 tatgaccaat ctatcccaag caatctagaa gagatattag taaagggaca tttactagaa
     7921 gtcttagatg agcctataaa tatatgttct gatggcgtca tcagtggtct gattgacgga
     7981 agcttaggtg tgatcgatct ctctggacca aacaacgaag gaatcaaggg gaatccggca
     8041 cgttaagaaa aaaatagcat aaaaaactag acacttgatg agcgttcatt caattgtttg
     8101 aagggactag gctggtgtca ttttttatac atatattttc tctttcgtct tgatagggct
     8161 tatgggataa tataaggaag aaaatcatcg ttttattcaa aaaggagttt ttgacaaatg
     8221 aaaaaacgta tattatttat cggtgatagc attacagacg caggaagaga ccgagcagaa
     8281 gcaactgatc taggtaaagg ttatccactt ttgattgccg gaaaactaat ctcccaatat
     8341 ccaacgatgg aatttgaatg catgaaccgt gggataggag gagacaaaat tgcagatctg
     8401 caagtacgct ggcaagaaga ttgtttagct ttacaacctg actatgtatc gattttgatc
     8461 ggaatcaatg atacctggca taatactagc gatcaggcta tattcggaac tgagaaatct
     8521 gctcagcaat ttgaatccgt ttatcgaaaa ttactaaatg aacttcaaac agtgggaatc
     8581 tgcaacatca tcttgatgga gccgtttgta cttcctataa ccgacgaccg tcatggctgg
     8641 cgtaaagact tagaccctaa aatccaaatt gttcgtaaac ttgctaagga atatggcgca
     8701 ttgctggttc ctttagatgg tatattcaat gctttaggga ttgtttatgg tttttcgaaa
     8761 tatacaagag aagatggggt ccacccaacg attggcggac atgagatcat tgcacaaagc
     8821 tggatagatg caattgccgc agtcgatttt tttagtggag ataaaaatga gcgaaagaaa
     8881 agtgagaaat gatggaaata cggtgggcag taaaagaaga tctacctcaa atcaatcagc
     8941 tgattactga tcatccagaa tcattcgtaa aagcaaaaac ggcagctgac ctaaatgata
     9001 agaataaact attgctcgtt gctgtaacaa aagaaaaacg gattatcgga tttgcgcaag
     9061 cggacgtgca acagtcagaa gaacgattgg atcagcgacc atcaggaaaa ctaatccaac
     9121 acacattagt aacaaaagaa aatacggaag aaatccaaaa aaaatttgca gaatatttga
     9181 tactggaagc taaaaaaaga agaataaaat cattttttgc ccagttttaa tcaaaaacag
     9241 taatagaagg agaaatacaa tgagttgttt aggaaatgtc atctggttta tattcggcgg
     9301 actaatcggt ggtcttagct ggttagcagc ggggatactt tggtgtatca cgatcatagg
     9361 tatcccaatc ggtatgcaat gttttaagat cgcctccctt tcttttgcgc cttttggcaa
     9421 agaagtaatt tatcaccagt ctggcatgtc attgattgtt aatatcatct ggctgatcgt
     9481 ttcaggccta ccaattgctc tcgcacatgt cgtcagcggc atccttctat gtatcacgat
     9541 catagggata ccttttgcca agcaatcttt caaacttgct cgattggcgt tgatgccatt
     9601 cggtgcagaa attgtataaa agagaataat aaaggttaga taaagcatag agtcaggtgg
     9661 gatacatgta tttcacctga ctctatgcgt tctcctgtat aagttcttcc atgtgacttt
     9721 tacgtctttt atttatatgg ggttactgtt tatggaaagt tgaaataatt ttcattaaag
     9781 aactctttct atatttttga aagcgtagtg gagcttcgaa tatctgggaa ggacattttg
     9841 cagggattga aaaaagatat tgtcctatcg aaaaattaat gaatttagac aaattgccag
     9901 caacaggctt caaacttagt tgctatcctg tcaagatcaa gaaggcaagt gcgggttgga
     9961 taagagcagt ggcaatgatc gaaaagaaaa agaaagaata agccaaaaca tctgtatgga
    10021 agaaattttc cgatgcagat gtttttcatt ttttctttcg gataccttga catatgaata
    10081 accgttcatt tataattagt tatgaagaga tattcatatg ttgaggcgag gaggtcatta
    10141 aaatgaaaag ttacagatta gaaggtttag attgtgcgaa ctgtgcaatg aaaatcgaaa
    10201 aaggagttca gaagatcaat ggagtcaagg aagcaacggt aaacttcact tctggaaaac
    10261 taacgattga cgcagaggaa gatcatttgg ctacgattga acaagaaata aaaaaagtag
    10321 tgaaggaatt agaaccagat gtcaaagtaa cagaaataga taaggaaaaa gtatctgaac
    10381 atggaaatga aaaagagaga aatacgctgt tccggatttt attttccctt gcaggtatcg
    10441 ctttattgct attatttgat tttaatgaac caattcgctt gatagggtat ttattgattt
    10501 atctgctgat aggatatgat gtagtgaaga aggcagtgat gaatatcgtc aaaggaaaaa
    10561 tctttgatga gaattttctt atgtctgttg ctacgattgg cgctatgttg atcggtgaat
    10621 atccagaagc cgtagcagtg atgctatttt atcaaatcgg cgagtatttc caaggattgg
    10681 cagtcagtca ttcaagaaaa tcgattcgcg aattgatggc gatccgtcca gaaacagctc
    10741 acgtgcagac gacggaagga ttgatgaccg tgaaccctga agacgtgcag atcggtcagt
    10801 ttgtacttgt gaagcctggt gaacgtgtac cgttagatgg aacgatcatc gaaggagaat
    10861 cattagtcga tacgtcagca cttacaggag aatcagtccc taaatctgta tatgttggtg
    10921 aaacagtatt aagtggattt atcaataaaa ataaaccatt tttagttcaa gtagaaaaat
    10981 cttatgaaaa ttctactatc agcaaattat tagaattagt agaaaatgcc agcagtaaaa
    11041 aagcaccagc tgaaaatttc attactaaat tcgcgcgcta ttacacacca gtcgttgtag
    11101 gattggccgt tttattagct gttttacctc cacttgttgt ttctggagcg gcgttttctg
    11161 aatggatcta tcgagcattg acatttttag tgatctcatg tccatgtgca ttagtgatct
    11221 ccgtacccct tagtttcttt ggcggtatcg gaggagcaag taaaatcggt gtattagtaa
    11281 aaggttcaaa ttacttggag ttgcttgcac aaactgagac agtggtattc gataaaaccg
    11341 gcacattaac gaaaggtgac ttttcgatcc agacgatcga tacaaaaatc gatccaaaga
    11401 tattcatgca atatgttgcg agtgcagaac agttttctac acatccgatt gctcaatctg
    11461 ttttagaagg ctacgcaggt ccgcttttac caacagctaa tatccaagaa tttgcaggtg
    11521 aagggatctt agctgaagtt gatggcaaac aagtattagc aggtaatcac aaattgatgg
    11581 aaagatttga aatctctttt ccttcaagtc aagaaatcgg tacattgctt tatctagcga
    11641 tcgatcaaag ttacagtggt tatcttgtta ttgcagatac cttgaaagaa gatgccgtag
    11701 acgcattggt tcaattaaaa aaggctggtg tgaagaacac agtcatgttg acgggcgatt
    11761 cgaaaaaaat tgctgatcat atcgggaagc aagtaggcgt ggacaagata tatagcgaat
    11821 tgcttccaga agataaagtg cagcggttag aggaaatctt acaaagaaac aataaaaaga
    11881 ccgcatttgt cggagatgga atcaacgatg caccggtgct agcaagagca gatgtaggaa
    11941 tcgcaatggg tggattggga agcgatgctg caattgaagc agcagatgta gtcatcatga
    12001 atgatcaacc atcgaagatt gcagaagcaa tccatttagc aaagaaaaca ttgaaaatcg
    12061 tcaaacagaa cattgtcttt gctattggga taaaaatttt ggtacttgcc ttaggtgctt
    12121 ttggttttgc atcaatggga gatgcagtat ttgcagatgt tggggtgaca gtattagctg
    12181 ttttgaatgc catgcgaagc ttgcatgtaa aaaataaacg ttgatcaatg aagaggacga
    12241 atgaacgtcc tctttttttt atatcctttt cttaatgaaa aatgtaaaga aaatagatag
    12301 gattataaaa taactaatgt ttttaatttt gcagaaaatt caaaatagct tgaaattcaa
    12361 taaggtaaat gaaataaaag aaaataactt atttttttga tggaaaaaat atgaaaaaac
    12421 gatgtgtatt aaataaaaga atacacacat aaaataacac tgtcttgtaa gtttgttaaa
    12481 aattgaatat gattacatct ggataacaaa tttttatcat aaaggagaga acgaatgaac
    12541 aacaaacaat tacgctggtt tactgttgcg ttaattgcat ttaacatggt ttggggcatg
    12601 gggaatgtag ttaataacta tgctcagcaa ggagtatcag ttgtcacctc ttggctattg
    12661 attttggcca tttattttat tccatacgca ttgatcgttg gacaattagg gtcagccttc
    12721 aaggatagta aaggaggcgt cagttcatgg gtcgaaaata cgacatctaa taaacgactg
    12781 gcatactatg ccgcttggac ttactgggtc gtccatattc cttatttggc acaaaagcct
    12841 caagcaatcc tgattgctgg cggttgggca gtccaaggaa atggaaatat cgtaaatacg
    12901 atgtcagtac aaatggtagc agttatttca cttattatat ttcttgcctt tttatattta
    12961 tcaacaaaag gactttctac tttgaaagtg attggcggac tggccggaac agcgatgttc
    13021 gtcatgtcga tcttatttat tcttttagct gtaacggcac cgttcatcaa tccaacgatg
    13081 gaatttgcca caccacatat gaatcaagtg aaaacatata ttccaacatt tgatttcagt
    13141 tatttcacaa ccgtttcgat gctagtattt gcagttggag gagcggaaaa aatatcccct
    13201 tatgtcaact ctacaagaaa tcctgcaaag gaatttccta aagggatgat cttcttagca
    13261 gcaatggtag ggatttgtgc tgtattaggc tcagtcgcaa tgggtatgct gtttgctagc
    13321 gataatattc ctaaagactt gatggcaaat ggggcataca gtgcttttca aattttaggt
    13381 aattactatg gtatcggtaa ctcattgatg atcatttacg cattgacaaa catgattgga
    13441 caaatctccg cattagcctt ttcaattgat gcaccactaa aaatcttgtt agcagatgcg
    13501 gatccagaat ttgtaccaaa ctggctacgt aaacgtaatc gtaaaggtac attggtgaat
    13561 ggttatcttc tgacaggtat actagtagga atcattattt tgttgccgat ctttggtatc
    13621 aaagatatga atgaattagt aaaatggttg acgaacttga actcagttgt catgccaatg
    13681 cgttacctat gggtgttctt ggcatatatg atgttgaata aagcttataa gaaattcaat
    13741 tctgaatata aatttgttaa aaatccaaaa atcggtttta tctttggtgc ttggtgcttt
    13801 gccttcactg cgtttgcatg tatcctagga atggtgccaa aagtagatta tactgctgat
    13861 cctaaagcat ggtggttcca attattcacg aatatcatca caccaattgt cttgatctta
    13921 ttaggtatga tcttgccagc aattgctcgt cgagacaaaa acaaagaaat cactgaacca
    13981 gaaacagtgg ttcagcctta aaatagaaat acttgtta
//
LOCUS       DACEMK010000071        13979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEMK010000071 DACEMK010000000
VERSION     DACEMK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.073"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW715_001906"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW715_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1022510.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW715_001907"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW715_001907"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1022511.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW715_001908"
     CDS             complement(2169..2906)
                     /locus_tag="IW715_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1022512.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW715_001909"
     CDS             complement(2997..3524)
                     /locus_tag="IW715_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1022513.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW715_001910"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW715_001910"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1022514.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW715_001911"
     CDS             complement(4401..4886)
                     /locus_tag="IW715_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1022515.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW715_001912"
     CDS             complement(5039..5236)
                     /locus_tag="IW715_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022516.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW715_001913"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW715_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1022517.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW715_001914"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW715_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1022518.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW715_001915"
     CDS             9211..9423
                     /locus_tag="IW715_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1022519.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW715_001916"
     CDS             9425..11104
                     /locus_tag="IW715_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1022520.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IW715_001917"
     CDS             complement(11358..11558)
                     /locus_tag="IW715_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1022521.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IW715_001918"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IW715_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IW715_001919"
     tRNA            complement(12249..12322)
                     /locus_tag="IW715_001919"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IW715_001920"
     CDS             complement(12634..13032)
                     /locus_tag="IW715_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022522.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IW715_001921"
     CDS             complement(13025..13477)
                     /locus_tag="IW715_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022523.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13970)
                     /locus_tag="IW715_001922"
     CDS             complement(13464..13970)
                     /locus_tag="IW715_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022524.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
BASE COUNT         4118 a         2943 c         2348 g         4570 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagagt acaggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaaggtgtg gcaacaatcc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catatatcat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaatact
    13861 tgtaaaaaat tatctgtaag atgttcatct tcaaagatag tttcttcgag atatttaatc
    13921 gccaattcta aattgctatc attttgttga accaaccact taatgtccat ctcactaaa
//
LOCUS       DACEMK010000072        13885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEMK010000072 DACEMK010000000
VERSION     DACEMK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.074"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            144..2309
                     /gene="acm"
                     /locus_tag="IW715_001923"
     CDS             144..2309
                     /gene="acm"
                     /locus_tag="IW715_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1022525.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2581..3066)
                     /locus_tag="IW715_001924"
     CDS             complement(2581..3066)
                     /locus_tag="IW715_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1022526.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3546..4187
                     /locus_tag="IW715_001925"
     CDS             3546..4187
                     /locus_tag="IW715_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1022527.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4206..4841
                     /locus_tag="IW715_001926"
     CDS             4206..4841
                     /locus_tag="IW715_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022528.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4859..5674
                     /locus_tag="IW715_001927"
     CDS             4859..5674
                     /locus_tag="IW715_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1022529.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5993..7429
                     /locus_tag="IW715_001928"
     CDS             5993..7429
                     /locus_tag="IW715_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1022530.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7618..10065
                     /locus_tag="IW715_001929"
     CDS             7618..10065
                     /locus_tag="IW715_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1022531.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10295..11362
                     /gene="rlmN"
                     /locus_tag="IW715_001930"
     CDS             10295..11362
                     /gene="rlmN"
                     /locus_tag="IW715_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1022532.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11555..12658)
                     /locus_tag="IW715_001931"
     CDS             complement(11555..12658)
                     /locus_tag="IW715_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1022533.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13211..13759
                     /gene="aac(6')"
                     /locus_tag="IW715_001932"
     CDS             13211..13759
                     /gene="aac(6')"
                     /locus_tag="IW715_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1022534.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4756 a         2300 c         2940 g         3889 t
ORIGIN      
        1 ttatttttta taaatatttg taaaataact atcaattttt tagttaaaag attggaaatg
       61 agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt ttatttttag
      121 ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta tcaatgttat
      181 ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag
      241 atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa
      301 acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc
      361 tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc
      421 cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg
      481 ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga
      541 tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga
      601 gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg
      661 ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt cgatggtttt
      721 tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt gatgaaatcc
      781 aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac
      841 agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc cccagcgcta
      901 catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa aactttgtca
      961 atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa caaataaatt
     1021 ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt gatggagaaa
     1081 gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt acagttagag
     1141 gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca aatgtgactt
     1201 tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc ctgttgactt
     1261 cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat gttgtaaaag
     1321 aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa ctcaaattct
     1381 ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag aaaaaggtta
     1441 cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg agtatttatt
     1501 ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca gaaacaaaac
     1561 gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat gatgatagtg
     1621 gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt
     1681 caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa gaagtcattt
     1741 cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa cgaccgacga
     1801 cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa
     1861 aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg caagaaatca
     1921 tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac gggtataaca
     1981 tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc catataacac
     2041 cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta ttctctcaaa
     2101 aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat tatattagta
     2161 agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct aaaacaagta
     2221 gtaaaaaatc tagttttgct gtaatcattg gtttattgat agtaactatc tgtgctggga
     2281 tattcttaca aaaatataaa aaagtttagt tagaatataa tattttttgt taaatatgac
     2341 tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt ttatatatgc
     2401 ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa tactcgtatt
     2461 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
     2521 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
     2581 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
     2641 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
     2701 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
     2761 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
     2821 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
     2881 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
     2941 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
     3001 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
     3061 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
     3121 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
     3181 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
     3241 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
     3301 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
     3361 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
     3421 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
     3481 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
     3541 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
     3601 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
     3661 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
     3721 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
     3781 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
     3841 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
     3901 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
     3961 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
     4021 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
     4081 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
     4141 caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
     4201 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
     4261 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
     4321 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
     4381 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
     4441 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
     4501 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
     4561 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
     4621 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
     4681 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
     4741 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
     4801 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
     4861 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
     4921 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
     4981 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
     5041 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
     5101 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
     5161 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
     5221 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
     5281 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
     5341 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
     5401 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
     5461 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
     5521 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
     5581 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
     5641 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
     5701 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
     5761 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
     5821 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
     5881 aactaagcga ctttttcaca acctccttta catattttta ttttgttgtg gaaggggttt
     5941 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
     6001 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
     6061 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
     6121 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
     6181 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
     6241 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
     6301 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
     6361 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
     6421 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
     6481 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
     6541 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
     6601 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
     6661 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
     6721 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
     6781 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
     6841 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
     6901 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
     6961 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
     7021 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
     7081 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
     7141 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
     7201 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
     7261 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
     7321 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
     7381 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
     7441 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
     7501 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
     7561 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
     7621 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
     7681 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
     7741 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
     7801 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
     7861 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
     7921 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
     7981 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
     8041 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
     8101 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
     8161 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
     8221 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
     8281 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
     8341 gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     8401 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     8461 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     8521 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     8581 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     8641 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     8701 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     8761 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     8821 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     8881 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     8941 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     9001 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     9061 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     9121 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     9181 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     9241 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     9301 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     9361 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     9421 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     9481 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     9541 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     9601 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     9661 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     9721 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     9781 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     9841 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     9901 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     9961 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
    10021 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
    10081 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
    10141 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
    10201 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
    10261 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
    10321 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
    10381 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
    10441 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
    10501 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
    10561 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
    10621 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
    10681 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
    10741 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
    10801 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
    10861 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
    10921 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
    10981 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
    11041 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
    11101 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
    11161 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
    11221 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
    11281 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
    11341 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
    11401 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
    11461 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
    11521 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
    11581 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
    11641 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
    11701 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
    11761 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
    11821 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
    11881 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
    11941 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
    12001 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
    12061 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
    12121 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
    12181 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
    12241 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
    12301 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
    12361 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
    12421 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
    12481 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
    12541 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
    12601 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
    12661 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
    12721 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
    12781 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
    12841 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
    12901 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
    12961 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
    13021 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
    13081 tttttatttt ataagtaaga taaataatga aagaatagag atactgattg gtagctaaag
    13141 atttttgagc gagaacattt cgttttttca aaaggactgt tagaataagc aagaacaagg
    13201 gggaagacgt atgataatca gtgaatttga ccgtaataat ccagtattga aagatcagct
    13261 ttctgattta ctgagactga cttggccgga agaatatgga gacagctcgg cagaagaagt
    13321 agaagaaatg atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt
    13381 aggatttatt ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt
    13441 tgtagaaagc tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa
    13501 agaagtagct tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg
    13561 aacaacgtta agtcaaacgg acctgtatga gcatacattt gataaagtgg cttccatcca
    13621 gaaccttcgt gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt
    13681 cttaccaaat gcaaatggct gggacaaacc ggatatttgg atggcaaaaa cgattattcc
    13741 tcgaccaaat tctcaataat aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata
    13801 agaaggaatt tacgaaaatt acttcttgaa tttttgtgaa attccaactt atttccgaag
    13861 aagctacttt tggaacaccg tgtac
//
LOCUS       DACEMK010000073        13874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEMK010000073 DACEMK010000000
VERSION     DACEMK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.075"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(6..662)
                     /locus_tag="IW715_001933"
     CDS             complement(6..662)
                     /locus_tag="IW715_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1022535.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            855..1133
                     /locus_tag="IW715_001934"
     CDS             855..1133
                     /locus_tag="IW715_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022536.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1152..2360
                     /locus_tag="IW715_001935"
     CDS             1152..2360
                     /locus_tag="IW715_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1022537.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2508..2873)
                     /locus_tag="IW715_001936"
     CDS             complement(2508..2873)
                     /locus_tag="IW715_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1022538.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3020..3793)
                     /locus_tag="IW715_001937"
     CDS             complement(3020..3793)
                     /locus_tag="IW715_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1022539.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3967..4827)
                     /locus_tag="IW715_001938"
     CDS             complement(3967..4827)
                     /locus_tag="IW715_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1022540.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4827..6245)
                     /locus_tag="IW715_001939"
     CDS             complement(4827..6245)
                     /locus_tag="IW715_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1022541.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6302..6751)
                     /locus_tag="IW715_001940"
     CDS             complement(6302..6751)
                     /locus_tag="IW715_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1022542.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7196..7459)
                     /locus_tag="IW715_001941"
     CDS             complement(7196..7459)
                     /locus_tag="IW715_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1022543.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7558..8853)
                     /locus_tag="IW715_001942"
     CDS             complement(7558..8853)
                     /locus_tag="IW715_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1022544.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8850..10121)
                     /locus_tag="IW715_001943"
     CDS             complement(8850..10121)
                     /locus_tag="IW715_001943"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1022545.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10286..11155)
                     /locus_tag="IW715_001944"
     CDS             complement(10286..11155)
                     /locus_tag="IW715_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1022546.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11447..13057)
                     /locus_tag="IW715_001945"
     CDS             complement(11447..13057)
                     /locus_tag="IW715_001945"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1022547.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3948 a         2921 c         2369 g         4636 t
ORIGIN      
        1 gttcatcaaa agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg
       61 tctttaacgg tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta
      121 gcagttgaga tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc
      181 atcccttctt ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca
      241 gctcccagct ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga
      301 tagcggtaat actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa
      361 aagcgcacac gattgattcg ataaaactct gcatattctg attcaactcg aatattatac
      421 ggcgaatcga tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg
      481 atatagcgaa ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc
      541 tctttcaaga tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat
      601 cctttgcgta taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc
      661 aatgatgctc ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt
      721 taaagtccaa aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac
      781 ttatgcgatt tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa
      841 tagagggaaa aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat
      901 atcaaacaag atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact
      961 cgatcgaatt gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa
     1021 atctgtttat gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca
     1081 attatcacga tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat
     1141 gtataacata catggaaaag aaattattta ataaatattt tattggaatc acattgatca
     1201 attttattgt ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag
     1261 acaccttaca cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa
     1321 cattgctggc acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct
     1381 taagaggcgg cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta
     1441 tcggggcatt gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag
     1501 ctacaaatgc tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt
     1561 attatggatt aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc
     1621 tgaatatgac tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta
     1681 cgattgcctg ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat
     1741 ctcttcgctc atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa
     1801 ttgccttttt gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa
     1861 aagtaatcca cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca
     1921 catttacacg tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt
     1981 atcctagcta tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg
     2041 tgacattgct tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg
     2101 gacaggctgt ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct
     2161 actttattgg tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat
     2221 ctcttacttc atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag
     2281 cactctatct tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat
     2341 cattgaaaaa tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa
     2401 taatctgagc ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt
     2461 tatttttaaa gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc
     2521 cgttcatctg gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca
     2581 ccgatcaata gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa
     2641 tatagataga gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg
     2701 ttccctactt tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt
     2761 accccaacaa tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac
     2821 agaatcgccc agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc
     2881 actcctttct cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat
     2941 accatcggct gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt
     3001 agaacattca cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg
     3061 catactgttc ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa
     3121 tggcttgatg catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt
     3181 ctgattgttt tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc
     3241 cttggctgaa atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg
     3301 catcgttata ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca
     3361 atacagatac ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg
     3421 taatgatctg caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac
     3481 tggaatccaa gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct
     3541 tacgtttttc tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt
     3601 ttcgattgtc aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca
     3661 gaaaaacaag gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg
     3721 tgactcttgc aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca
     3781 cttcattcaa cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt
     3841 gacagaagtg attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac
     3901 atcgtgtgtg gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt
     3961 ttatttttag tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac
     4021 cacttcttgt acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg
     4081 attttcactc aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa
     4141 attcactttg gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga
     4201 accgccatgt aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat
     4261 atttaatttt ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc
     4321 aagtgtatcg attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat
     4381 gattgtttca gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc
     4441 cactgagaca tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc
     4501 gtcgaatgat ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg
     4561 taagacatct tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt
     4621 ataagcatag gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg
     4681 atgaatttct aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat
     4741 catatcatag ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt
     4801 caataactct ttcattgtat ataacattac gcttccacct catttgttag attttcattt
     4861 ttcgtccact tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag
     4921 gctgtagtga ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca
     4981 tttggaatta gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc
     5041 atggaaatcc ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg
     5101 tcagataccg caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat
     5161 gattgacctg aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa
     5221 gcaataccta ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact
     5281 ccagctgaca tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata
     5341 tcagctgcca tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag
     5401 ctattcagcc aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat
     5461 accatcaaaa gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata
     5521 gcagaaaatt ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct
     5581 agcacgccac caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg
     5641 gcaacaattc ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct
     5701 aacgcaggaa ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct
     5761 aacacattat agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt
     5821 gcgatgatga ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa
     5881 tgattataga ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg
     5941 aatacgccaa tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg
     6001 cttgtagaga cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct
     6061 actgcaagaa tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt
     6121 tttacacctt gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat
     6181 ttttctgccg acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt
     6241 ttcatgatta ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt
     6301 ttcattcttg actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct
     6361 ttctaaaatc tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat
     6421 ctgctgattt ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc
     6481 cgctatagtc cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt
     6541 tgctttttgc atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc
     6601 gtttgaatag tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca
     6661 catcccctat tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca
     6721 gttcttctgc gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta
     6781 acgttttctt ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa
     6841 tgaacagagg aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc
     6901 tcacaaacaa tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc
     6961 ttctaatttt aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat
     7021 aaggtgtttt tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca
     7081 ttatgatcct acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa
     7141 aaacagacta tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt
     7201 ccgcagcgtt tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg
     7261 cttgtgtgaa tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac
     7321 ggatgactgg gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg
     7381 aacctgacaa gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg
     7441 gatggatgtt ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag
     7501 agttgatttg tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta
     7561 tcttttcaga tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa
     7621 aaattgctga ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa
     7681 tgaatctcca gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat
     7741 caataattct tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg
     7801 ttcagataat tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata
     7861 gatcacgtca tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact
     7921 gccaccttct tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc
     7981 tgggtcaatc ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg
     8041 agcaagtcta gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg
     8101 cattgcgcgt tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa
     8161 tgtagaagat accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg
     8221 ttcatcaatc agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc
     8281 gatttctttt aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg
     8341 accaaaaccg attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt
     8401 ttccagttga agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc
     8461 ttttgcttct tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg
     8521 ttccgattct ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa
     8581 tcgtctgatt tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg
     8641 ccgtaaaaaa ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa
     8701 tccttgtttc tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt
     8761 actgtaataa ggaattttaa attctttggc ataactataa acttctttta acgttttgtt
     8821 ctctagttct gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca
     8881 attgatgccc ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa
     8941 tctacattgt aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca
     9001 cctgcccgaa gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg
     9061 atgatcatat ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg
     9121 ttcacacgtt tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt
     9181 aacgcagtaa gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg
     9241 tttgcgggtt ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata
     9301 tggacaccaa tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt
     9361 ttcactttga taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg
     9421 cggtcaggaa tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc
     9481 acatctgtac ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct
     9541 atgatttccg gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca
     9601 gctaacatca cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca
     9661 cgcaatcctt tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca
     9721 cctagagctt caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca
     9781 cctggcaggc ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa
     9841 gaagcacgca aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga
     9901 tcgatttcta gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca
     9961 attaatgaat gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct
    10021 aagattgcag ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca
    10081 ccagctaaag gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat
    10141 taatcttttt tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg
    10201 gtctgccgtt ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta
    10261 tatatcgtat ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat
    10321 gagatttagc atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga
    10381 atcctttacc ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg
    10441 ataattggaa ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga
    10501 tttgatcttg aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag
    10561 catcagcaat tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac
    10621 catggatgtt accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc
    10681 attcttcaat atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg
    10741 aaccaacttc acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag
    10801 ctaatttcaa gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc
    10861 ctaattcgat acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg
    10921 gaacagtgat gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac
    10981 cacccatgta tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt
    11041 cagccgcttc aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag
    11101 cgtaaccgcc tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa
    11161 aattcctcct aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta
    11221 ttttaccaaa catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa
    11281 gttttttgca aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc
    11341 tatctttttt cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc
    11401 ttatcccctt ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt
    11461 gatctgctaa tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag
    11521 aaatcaattc tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga
    11581 tttctactaa acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca
    11641 attgacgata tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg
    11701 ctccatcata tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa
    11761 ggcgtaaagt tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat
    11821 tgtttgctgt atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac
    11881 gtgcaaattc gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat
    11941 tttcgcgagc ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac
    12001 caggaaccaa gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt
    12061 cttgtgcttt gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca
    12121 atgcttcaac tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa
    12181 tccgtacttt tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt
    12241 ctgcttctgg tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt
    12301 gtaaaactaa tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg
    12361 gttcgacatc acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg
    12421 tacgtaccac caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct
    12481 gcgttggttt agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga
    12541 tatacataac attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg
    12601 gcaaagattc tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag
    12661 tcattttagc tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt
    12721 gaacagttgc tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt
    12781 ttcctgtcgt cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc
    12841 ccagatccaa atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg
    12901 ggctcatcgt ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta
    12961 agccgcggtt tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg
    13021 aaacgacacc gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat
    13081 ttatgtgcag gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg
    13141 gagccgaaat ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa
    13201 gctgactaag ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt
    13261 tcgatatcct ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc
    13321 acataataag cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt
    13381 actgaaaaat tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa
    13441 tatcccacct aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc
    13501 gtagaaacaa tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca
    13561 cagttctcgg atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc
    13621 actaattttt tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa
    13681 ggtaacgaat cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt
    13741 ctaaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca
    13801 tgagaagctg tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc
    13861 tctggataaa cggt
//
LOCUS       DACEMK010000074        13852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEMK010000074 DACEMK010000000
VERSION     DACEMK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.076"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW715_001946"
     CDS             168..362
                     /locus_tag="IW715_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022548.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IW715_001947"
     CDS             631..930
                     /locus_tag="IW715_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022549.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IW715_001948"
     CDS             complement(1027..1461)
                     /locus_tag="IW715_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1022550.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IW715_001949"
     CDS             complement(1693..2403)
                     /locus_tag="IW715_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1022551.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IW715_001950"
     CDS             complement(2393..3253)
                     /locus_tag="IW715_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1022552.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IW715_001951"
     CDS             complement(3258..3896)
                     /locus_tag="IW715_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1022553.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IW715_001952"
     CDS             complement(3911..4210)
                     /locus_tag="IW715_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1022554.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IW715_001953"
     CDS             complement(4253..5737)
                     /locus_tag="IW715_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1022555.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IW715_001954"
     CDS             complement(5757..6218)
                     /locus_tag="IW715_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1022556.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW715_001955"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW715_001955"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1022557.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IW715_001956"
     CDS             7521..8291
                     /locus_tag="IW715_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1022558.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IW715_001957"
     CDS             complement(8564..9391)
                     /locus_tag="IW715_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022559.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IW715_001958"
     CDS             9959..11194
                     /locus_tag="IW715_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1022560.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IW715_001959"
     CDS             complement(12193..12453)
                     /locus_tag="IW715_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1022561.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IW715_001960"
     CDS             complement(12480..13781)
                     /locus_tag="IW715_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1022562.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4459 a         2833 c         2179 g         4381 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta at
//
LOCUS       DACEMK010000075        13691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEMK010000075 DACEMK010000000
VERSION     DACEMK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.077"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(221..1177)
                     /locus_tag="IW715_001961"
     CDS             complement(221..1177)
                     /locus_tag="IW715_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1022563.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IW715_001962"
     CDS             1281..1883
                     /locus_tag="IW715_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1022564.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IW715_001963"
     CDS             complement(1971..3470)
                     /locus_tag="IW715_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1022565.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IW715_001964"
     CDS             complement(3486..4010)
                     /locus_tag="IW715_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022566.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IW715_001965"
     CDS             4308..5057
                     /locus_tag="IW715_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1022567.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IW715_001966"
     CDS             5048..5833
                     /locus_tag="IW715_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1022568.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IW715_001967"
     CDS             5802..6518
                     /locus_tag="IW715_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1022569.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IW715_001968"
     CDS             complement(6620..7639)
                     /locus_tag="IW715_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1022570.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IW715_001969"
     CDS             complement(7658..8716)
                     /locus_tag="IW715_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1022571.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW715_001970"
     CDS             complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW715_001970"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1022572.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10161..10997)
                     /locus_tag="IW715_001971"
     CDS             complement(10161..10997)
                     /locus_tag="IW715_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022573.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11181..12398)
                     /locus_tag="IW715_001972"
     CDS             complement(11181..12398)
                     /locus_tag="IW715_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1022574.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12781..12864
                     /locus_tag="IW715_001973"
     tRNA            12781..12864
                     /locus_tag="IW715_001973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12815..12817,aa:Leu,seq:gag)
     gene            complement(13104..>13691)
                     /locus_tag="IW715_001974"
     CDS             complement(13104..>13691)
                     /locus_tag="IW715_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022575.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4138 a         2794 c         2365 g         4394 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat
     8941 gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat
     9001 acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag
     9061 ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag
     9121 tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat
     9181 tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca
     9241 tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct
     9301 ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca
     9361 gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat
     9421 ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc
     9481 gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact
     9541 tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa
     9601 ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt
     9661 gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg
     9721 attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa
     9781 cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga
     9841 ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag
     9901 ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg
     9961 ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca
    10021 agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt
    10081 ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag
    10141 tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag
    10201 ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc
    10261 acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga
    10321 ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta
    10381 taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg
    10441 atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt
    10501 ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta
    10561 gagtagctta atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg
    10621 aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt
    10681 tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa
    10741 caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc
    10801 atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc
    10861 ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa
    10921 agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac
    10981 tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat
    11041 tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt
    11101 tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact
    11161 gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc
    11221 ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc
    11281 tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc
    11341 aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata
    11401 taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag
    11461 atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga
    11521 taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg
    11581 gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg
    11641 accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta
    11701 acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact
    11761 aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg
    11821 atttcttcta ataattcttt tggataaagt gccccagtag gattattagg attaatgata
    11881 acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat
    11941 tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc
    12001 gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc
    12061 tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc
    12121 acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc
    12181 cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact
    12241 tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta
    12301 atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg
    12361 acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc
    12421 aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat actccatcct
    12481 agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt
    12541 gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc
    12601 acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac
    12661 acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc
    12721 tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac
    12781 gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg
    12841 tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt
    12901 ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga
    12961 acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt
    13021 agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac
    13081 taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata
    13141 ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt
    13201 agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc
    13261 cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc
    13321 attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt
    13381 ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga
    13441 taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc
    13501 caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca
    13561 aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt
    13621 tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc
    13681 atgtctaaaa a
//
LOCUS       DACEMK010000076        13540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEMK010000076 DACEMK010000000
VERSION     DACEMK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.078"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1010)
                     /locus_tag="IW715_001975"
     CDS             complement(63..1010)
                     /locus_tag="IW715_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1022576.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1200..1469
                     /gene="rpsN"
                     /locus_tag="IW715_001976"
     CDS             1200..1469
                     /gene="rpsN"
                     /locus_tag="IW715_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1022577.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1645..2007)
                     /locus_tag="IW715_001977"
     CDS             complement(1645..2007)
                     /locus_tag="IW715_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1022578.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2018..3139)
                     /locus_tag="IW715_001978"
     CDS             complement(2018..3139)
                     /locus_tag="IW715_001978"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1022579.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3155..3505)
                     /locus_tag="IW715_001979"
     CDS             complement(3155..3505)
                     /locus_tag="IW715_001979"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1022580.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3635..5146)
                     /locus_tag="IW715_001980"
     CDS             complement(3635..5146)
                     /locus_tag="IW715_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1022581.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5468..6820)
                     /locus_tag="IW715_001981"
     CDS             complement(5468..6820)
                     /locus_tag="IW715_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1022582.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6973..8049)
                     /locus_tag="IW715_001982"
     CDS             complement(6973..8049)
                     /locus_tag="IW715_001982"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1022583.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8245..9990
                     /locus_tag="IW715_001983"
     CDS             8245..9990
                     /locus_tag="IW715_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1022584.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10239..10706)
                     /locus_tag="IW715_001984"
     CDS             complement(10239..10706)
                     /locus_tag="IW715_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1022585.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10690..12063)
                     /locus_tag="IW715_001985"
     CDS             complement(10690..12063)
                     /locus_tag="IW715_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022586.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12384..12872)
                     /locus_tag="IW715_001986"
     CDS             complement(12384..12872)
                     /locus_tag="IW715_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1022587.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12894..13496)
                     /locus_tag="IW715_001987"
     CDS             complement(12894..13496)
                     /locus_tag="IW715_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1022588.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3864 a         2938 c         2458 g         4280 t
ORIGIN      
        1 attttttgtc tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt
       61 tcttattttt cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga
      121 tgtgcttgga tgacatgctt aagggaagca gacagattca ttgaaataaa aacatgaaag
      181 gctccttttt ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt
      241 cttggaacaa acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg
      301 tcatttaatg caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg
      361 atacttcctt tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga
      421 tttgttttat cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt
      481 ttactggatg cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt
      541 gacccgaccg cacccgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc
      601 aaatggttga ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc
      661 caactcattt tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg
      721 taagtcccgc cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct
      781 gaaccaatct ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat
      841 gttcgtatct ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat
      901 gcttttattt ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct
      961 tcaaaaacat cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc
     1021 tccctttttc tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt
     1081 tttggcgcat gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt
     1141 atctaaaagc gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga
     1201 tggctaaaaa atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg
     1261 ctgctttacg ccaggaatta aaagcgcaaa aagattacga aggcttgcgc aaacttcctt
     1321 tagatgctcg tccagaacga ttgaaaaatc gtgatttgat cgatggacgc cctcgcggat
     1381 acatgcgtaa attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga
     1441 ttccaggagt aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg
     1501 gcgcatggac cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg
     1561 ctggtactag aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa
     1621 ggctgcatgg ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac
     1681 actaattttc aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt
     1741 ttctttcaat cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct
     1801 ttctattcct gttccagtag ctttgatccc aatatgagtc ggaagcttag gtttagccat
     1861 ttttgccgtt atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg
     1921 aataaccaaa actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc
     1981 aaaataaata tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta
     2041 tccgctgtcc taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat
     2101 cctgcatagt gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag
     2161 gaaattcttg aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac
     2221 agtagttccc ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat
     2281 aaccgatcgg tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc
     2341 cgattccttc acctgctggg agttttttga cttgaattaa ttcagaaacc agctcaagtg
     2401 ctggctgcag tggataaact tccggtagtg cgtgcccaga gggattcagt ccgtacattg
     2461 ctactccata acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat
     2521 tgctgacatg tatatatcga ggaagttcgg gaagtttttt tagcacttct ataaagcgtt
     2581 ctttttgcag attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata
     2641 aaccttccca ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt
     2701 caggtgtaca aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag
     2761 gcgtctctag cttacttttc tctaaaacag cagctgctgc ctgcaaccat tcccgtgtac
     2821 ctgcagttac tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat
     2881 tcaagataag gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat
     2941 ttgaaacaca aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc
     3001 catgaccgta tccgtttgct tttactactg caaacaattc ttttccttgc ggaagtcttg
     3061 caacttcatt tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc
     3121 ggtgccaagc gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa
     3181 aagctgtttc atttgtatga gtaatactga cccatacttt gccttcatga ggtgaacgcg
     3241 tcacaacagg ctgaccgttt tcatttttta aaatctcgac atcttgaaag gtaacttttc
     3301 cgatcccagt tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa
     3361 attctacttg gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga
     3421 ttcgtgcaat gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg
     3481 catcgatccc aattcctttt atcatatgct tttctctttt caataaattt agaacactct
     3541 cattctacca aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag
     3601 tttctcttaa aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa
     3661 atccgcgttt tttatcgttg tgacgtttac cgccatcttt ttggctgtta ttatttttgc
     3721 tataaccgcc ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt
     3781 ttccgccacc acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt
     3841 ctggtgtgat cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa
     3901 gtgcaactaa atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtact
     3961 tatctagacc attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac
     4021 ctttgaatgc ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt
     4081 tttcaatcac atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc
     4141 cttttccggc acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga
     4201 tatcatagtt gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg
     4261 tcgctacaag gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt
     4321 gttgagacaa atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg
     4381 ccaattcgtc tacacggcgt tttgtacgtc cgaagacgat tgttagttca ggtgtttgaa
     4441 catcaaacaa gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt
     4501 gatcgatcaa gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca
     4561 tgaatttaac gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt
     4621 gtctttgatc aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca
     4681 acatttcatc tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc
     4741 ggttgatgtg gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca
     4801 agccacggat ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt
     4861 tgtcgcgccc taaacgatat aattcttctt gtgtttggat agctaactca cgtgttggtg
     4921 cgataactaa accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc
     4981 caaatgcagc tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta
     5041 atgctagtgg aatcgtttct gattggatag gtgttgcttc ttcaaaacca gcgcgttcga
     5101 ctgatttcaa caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat
     5161 atggttgtta actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg
     5221 tattgtttct ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac
     5281 caccgaaaaa tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc
     5341 tttatcatta ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat
     5401 ttctcttcat ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta
     5461 cagattttta tttcatttgc tgaagctgtt ctaccacttc aaataagccc atgctgttgc
     5521 tgccttttag cataatacta tctgttgctt caagattttg ttttacagca tcaatcaatt
     5581 tattcttttc tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt
     5641 cttttagtgc atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga
     5701 tatgttcact catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg
     5761 ctaacacagc gattctgcgt ccttttgttg gtagattttt aaaagtatct aataccagtc
     5821 ccatggctgt tggatttgca ttatagacat cgctcaaaat agctgcacca tttcctgccg
     5881 ttagccattc ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag
     5941 gtatggacag tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat
     6001 aacttccagg cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt
     6061 ttccttttgc tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg
     6121 tttgtactaa tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta
     6181 atccgtctgg tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg
     6241 acccaagatt ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag
     6301 ccataagtga aagttccgtg atttcattgg cgtgatccat gcccatttcc aagacgatct
     6361 tttctgtatc ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat
     6421 ttccttgcgt cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg
     6481 tggtggtttt accattgctt cctgtaattg caacgacttc tgcacctact ttttccaagt
     6541 aataggttgc caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat
     6601 cttttggagc ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg
     6661 cttgctggat aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt
     6721 cggttatctt ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc
     6781 tgcccacggc ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag
     6841 gttgtgaaaa aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa
     6901 tttctctatt cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcttctt
     6961 ctttatttct ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat
     7021 tcctaattga atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa
     7081 gagtgggtac atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa
     7141 ttcgttttta tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt
     7201 gtaagctaac ttcgcgtact cttgcgcttt ttgataaact tcttccggca cttcggctgg
     7261 aatctgcatt tcgattttat tattgatata tttggcttca taatcataga atgctacgtc
     7321 ttttacgact tcgccaggca aagtcgtccg aacatcttca tttcctaata cagcaacttc
     7381 gatttcgcgc gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt
     7441 tgctaaagca ttttgcagct cttctcggtt ttctgccttt gtaatgccga cactagaacc
     7501 catattcgca ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac
     7561 ttttttagga ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac
     7621 accagcagct tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa
     7681 tacgcctgcg ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc
     7741 ttccccgttt ggcccgtgta aaactggaaa aacgatggct ccttcttctt tgatttctcc
     7801 cggattgatc acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag
     7861 atgcaagaca tctttgctag caggtttttc tgttagtaat ggacctttga cccattgtcc
     7921 ttcttttgta ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac
     7981 tgaaaatgcg gaaagaatgg aaacttcatg ctctgcgctg cgtccgccat atagtaaagt
     8041 aatcttcaaa agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt
     8101 agcacaaaac aaaaataaac gaataactta tttataaaaa aacgagttgg tttctaaaag
     8161 aacaaacgtc atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta
     8221 tgataaagaa aaggagggat gatgatggcg caaacaaatc cacgtagccg cttggcagaa
     8281 atcattcgag tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc
     8341 gaacaggtaa aaaaagcctt tgaagaacta ggccccacct tcattaagat aggacagatg
     8401 ctttctgttc gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa
     8461 gatagtgtac ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct
     8521 ctttcattat cagatatatt cgatgatttt tccaaatcgc cttttgcctc tgcttctatg
     8581 gggcaggctc atcgcgccaa gctgaagaac ggtgactcgg tcgtcgttaa gatccaacac
     8641 cctaacatcg ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg
     8701 atcaaataca ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa
     8761 cgttccttga tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat
     8821 caaaagaaca atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg
     8881 aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact tgaaccattt aatgaacgcc
     8941 gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt
     9001 gcaaagcttt tagttgagaa ctttatgaaa caggtatttg atgatggttt tttccatgct
     9061 gatcctcatc cagggaatct gctattccat gtactaaaaa aagaagaaca gacacaagct
     9121 agccgaaaaa ctgaaatagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc
     9181 acttctacag aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg
     9241 gggcatcttt ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg
     9301 aaagatgctt atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt
     9361 gatgaaagtc ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct
     9421 attgatgaaa tcaacttgca agaagtattc acacacgtcg tgacgatttg tcatcaaaat
     9481 aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg
     9541 gtgatccgcg tccttgaccc tgaagtttcc ctcatggaag tcgcttcgcc ttttgcccaa
     9601 cattattttc tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta
     9661 ttggaaggga tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg
     9721 tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc
     9781 aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta
     9841 gggtcctccc tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta
     9901 gggatattta cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg
     9961 atccaaatgt ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc
    10021 aagaaataag aagtgatttt gcataaattg tttctcaaat ttttgtgaaa attcggctta
    10081 tttttcgaag aggctacttc tgaagcaccg agaataaaaa atgtgaaaaa gctgttctgc
    10141 acccagtaat aagaacaatc aaaaaggaac atggagcaca tttatcctgt ttccctgttc
    10201 cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt
    10261 ggtaaacctt ggtcaattgc cttttttaac gcttgacaaa aagacatatg atctttctcc
    10321 gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa
    10381 cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac
    10441 actcggacgc ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag
    10501 tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg
    10561 tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg
    10621 ataaccacgt caagaccata ggattctcct aattttatga catcttcttt tactggagaa
    10681 ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt
    10741 tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac
    10801 actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc
    10861 catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc
    10921 tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag ctgcatcagt
    10981 ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa
    11041 gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag
    11101 caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt
    11161 tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc
    11221 gtacattatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt
    11281 tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa
    11341 aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct
    11401 ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa
    11461 taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta
    11521 acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa
    11581 gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca
    11641 gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg
    11701 aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact
    11761 tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc
    11821 accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt
    11881 gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag
    11941 ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt
    12001 acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa
    12061 catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa
    12121 ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaatacaga aagaagcata
    12181 agcttctttc tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa
    12241 aagtcttgcc cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt
    12301 gcctccaaaa tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat
    12361 ttactttaac acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt
    12421 tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta
    12481 taaacctcga ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt
    12541 gtttgcgcag caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt
    12601 actttattta gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca
    12661 gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt
    12721 tctttttgaa tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga
    12781 ccaatgatcg gcacaaaagc ttccggccat acttcttgta tgactgccat cagctcatcc
    12841 agttcatttt ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa
    12901 tgaccttgcg cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccac
    12961 ggtttcaaga aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct
    13021 actagacaga tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt
    13081 ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat
    13141 aaaataagct ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc
    13201 ccaatcacta ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg
    13261 cccgctactg aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac
    13321 agaacaaata tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg
    13381 ctcgatcgaa aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga
    13441 ccaataatgg gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat
    13501 attccccaca tcttttttca tttattaaaa ctgcctcctt
//
LOCUS       DACEMK010000077        13048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEMK010000077 DACEMK010000000
VERSION     DACEMK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.079"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW715_001988"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="IW715_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1022589.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="IW715_001989"
     CDS             1873..4539
                     /locus_tag="IW715_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1022590.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="IW715_001990"
     CDS             4903..5655
                     /locus_tag="IW715_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1022591.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="IW715_001991"
     CDS             complement(5677..6831)
                     /locus_tag="IW715_001991"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1022592.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..8251
                     /locus_tag="IW715_001992"
     CDS             7016..8251
                     /locus_tag="IW715_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HAQ1022593.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKSFISLRHLNGAVN"
     gene            8251..9456
                     /locus_tag="IW715_001993"
     CDS             8251..9456
                     /locus_tag="IW715_001993"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1022594.1"
                     /translation="MEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIPMGIAQN
                     FQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKSEYQAVI
                     NAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDVKDAMGA
                     NMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNKEIGEQI
                     AKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAARNGLYQ
                     GLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQVIAAVG
                     LAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKMNQQTAI
                     QILEKIREK"
     gene            complement(9786..10490)
                     /locus_tag="IW715_001994"
     CDS             complement(9786..10490)
                     /locus_tag="IW715_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022595.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10677..10922)
                     /locus_tag="IW715_001995"
     CDS             complement(10677..10922)
                     /locus_tag="IW715_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022596.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10939..11790)
                     /locus_tag="IW715_001996"
     CDS             complement(10939..11790)
                     /locus_tag="IW715_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1022597.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11956..12960
                     /locus_tag="IW715_001997"
     CDS             11956..12960
                     /locus_tag="IW715_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1022598.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4168 a         2315 c         2716 g         3849 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa gttttatcag
     8221 cttacgccat ctgaacggcg cagtcaatta atagaaaaaa atgtattgac acaagaaact
     8281 gcgttgattt ttcaggaaca aacactttct gaagagctct ccgatcacat gatcgaaaac
     8341 caagtgagtg aagtggaaat cccaatgggg atcgcgcaga attttcaaat caatggcaag
     8401 aaaaaatgga ttccgatggc aacagaggaa ccatctgtca ttgcagctgc aagtaatggt
     8461 gcaaaaatct gtggaaatat ctgcgcagaa acacctcagc ggctgatgcg agggcaaatc
     8521 gttctatcag gaaaatcgga gtatcaagca gtgatcaatg cagttaatca tcgaaaagaa
     8581 gagctgattc tttgtgcaaa tgaaagttat ccttcaatcg tgaaacgcgg gggcggtgtt
     8641 caagatatct ctactcggga attcatgggg agtttccatg catacttatc tattgatttt
     8701 ttagtggatg taaaagatgc aatgggcgct aacatgatca actcgattct tgagagtgtg
     8761 gcaaacaagt tacgggagtg gttccctgaa gaagagattc tattcagtat tttaagtaat
     8821 tttgctacag aatcattggc ttctgcctgt tgtgaaatcc cattcgaacg cttagggaga
     8881 aacaaagaaa tcggtgaaca aattgccaaa aaaatccaac aggcagggga atatgcgaag
     8941 cttgaccctt accgtgcagc aacgcacaac aaagggatca tgaatggtat agaagctgta
     9001 gtggcagcta caggaaatga tacacgtgcg gttagcgctt cgatccacgc ctacgcggca
     9061 agaaatggat tataccaagg attaacagac tggcagatca aaggggataa actagttgga
     9121 aaactaacgg tgcctttggc tgttgccaca gtaggcggag cttccaatat cctgccaaaa
     9181 gcaaaagcat ctttagctat gttagatatt gattcagcca aggaactagc acaagtaatt
     9241 gctgcagtag ggttagctca aaacctggct gctttgcgtg cattagttac agaaggaatc
     9301 caaaaaggcc acatgggact tcaagctcgt tcattagcga tctcaattgg ggctattggt
     9361 gaggaaatcg agcaagttgc gaaaaaatta cgtgaagcag aaaagatgaa ccagcagaca
     9421 gctatacaaa tattggaaaa aatccgggaa aaataaaaaa gcaatatctt tagaagaata
     9481 aagcaagatt tacagttatt ttatttactg cagatcttgc ttttttctct atcagaaaat
     9541 atgtttatta ttagaatttt ttatagttaa ggttaggcat cttaagaaat caggcaactc
     9601 caagatatct tcaggtagaa gagaagtcgc ctaacattgt aggatcatag gtgatttgtt
     9661 ggacaggtca agctgatatt tgagcctgat gttagaagga atttcttttt acttgctgta
     9721 gaaattatta tatgactaaa aaggctggga ttttaaatcc cagcctttta tgacaaatag
     9781 aatttttatc gatcagtaat cgctgtcgct gcaaattgtt cgatcaaagg aatcaaagtc
     9841 aatttgactt tgtatttccg ttcgagtgct ttttcgataa cgaactcatt ttcagtagtc
     9901 atagaaatag ttgctaattt acgaggttca tcagcatttt tttcattaat aactacttca
     9961 tgttcatttt gttctgtgta aaatttagga tactcagcaa cggcttttga aaaatcagca
    10021 aaacttaaag cggcattcgt atcagttgag cgtgtatttt cagttgtgtc cgcttttggt
    10081 ttttccgttt tttcttctgg cacactgact gtttcttctg taactggtgt cgcaacagct
    10141 tgcgcagttg gttcatcgtt tttcaatcgt tgatcattcc agtaagacac gatttcaata
    10201 agaagattgt cacggaatcg ttcgaaaatc ccgttttctc ctgaacgaat tctctttaat
    10261 acagtattga agttgtcatc gattggcacg ttgatccgtg tatcttcatt tgttttccca
    10321 gctaatccga tcgttaagat ttgacggttt ttgggatcaa gcatgacttg taacggcaaa
    10381 ccataacgtc gcaagctctc ttcttctaga gaacggaata attgtcctct tacaccatcc
    10441 aatgaaggac ggaattgatt taataatttt tgtttctgtt ttttcttcat atgttctctc
    10501 cttcgctcat accctttcga ttataagaaa aaaacagcca aatgaaaagg agtaatgcaa
    10561 aaaaacttgc gtatacgcaa attacaacag aaaaatttgc gtattttccc gttttttgaa
    10621 agaaaaataa aaaatggaac ggattatgta tatatacata atccgttcca ttctttctat
    10681 tttttcggta tcaaggcaaa aacaggtagt tcaggtgaat aaatgacttc atgttcgtgc
    10741 cagggtttga ccattagcga atgatcgaac cgttttttca gttcttcgat gatttgatca
    10801 tgtgtccagt ttcttgcagg aaaatgttgg attttctctg catcgatcaa aaagacaaaa
    10861 tcggaatgaa aagtctgggt aacttcttcc gagagagaat tcaagcgttc gattggatcc
    10921 ataaaatcac ctttcgactt aaaattgggt agtatctggg tcttttgctt tgccgactac
    10981 accatctaac tgatcgattt gtttcatttc ttcttctgtt aattcaaaat caaataattc
    11041 agtattttct ttgattctgc ttggtgtcac tgacttagga agaggaagga attcgtgttg
    11101 cagcgaccaa cgcaaagcaa cttgtgcaat cgtcttgtca tgtgcttcag cgatttgttt
    11161 catttcaggc acatcgaaga ttttaccagt gcctaatgga ctatatgctt cgaggatcat
    11221 atcatgtttt tttgcatact caactacagt tgtctgtaat tcgcctggtg caaggaaaac
    11281 ttgattgacc atcggcataa tttttgctgt ttttgctagt gtatctagat ggtggggtaa
    11341 gaaattgctc acaccgattg ctttgatttt acctgcttca tacagttctt caaatgctcg
    11401 ccatgtatct gcatttgctt gttcccaatt atcacgaaaa gcaacagggt ttggccagtg
    11461 gatcaaaaac agatctaaat aatctgtttg gagcttcttc aatgattctt caaaagaaga
    11521 catcacaagt tcatagctgt ggtttgcatt ccataatttt gttgtcaaga atatctcttc
    11581 tctaggaatt ccactttcct taatcgcttg gccgacgctt tcttcattct tgtatccttg
    11641 ggcagtatcg atatggcgat aacctgcagc cagcgcttct ttaacagagg aaacagcgac
    11701 atccccatca ggtgtttgcc atgtgccgaa tcctactacc gggatttcgt aaccatttga
    11761 tagacgataa gtgcttgtta atgaattcat actttcagct ccttttttat tcgttcaact
    11821 ataagtgtag cgcatttttc gaaaaaaaga atccattttg tttctactct tgaaattttt
    11881 gtttaaaaga aaatgatttt gattctggta tttttgattt tttttccgta aactaagaca
    11941 gaagggaatg agaaaatgga tatctattta aaaaaagcaa ttctgcatat tattgacaga
    12001 gaaactggtt ctccagtctt ttcacaaaaa gagctggatc tgacaaaaga atatattcgt
    12061 gattttcttc aaaagaagat ccaaaaaatc tcttctgctc aaacaaaaac tggacaacta
    12121 gcagaagaaa gcatgatcgt tgaatcactt aaaatcgctc aagaaaattt tgtggaagca
    12181 agtgaagcaa tcgttcagcg gtggttcgat atctaccaag aaagtgaaga agcacctagt
    12241 gcagatgtat ttgttgtctt gtatgaatta gatacagtga tgtacttatc tttattgaaa
    12301 gtcaattatc gtgaggctta cactcacttt gttgaagcag acgaagcagg gatcgacaac
    12361 cgcttgattt tgaatcgagc aattctaggg gcgaaaacac aaaaagcaga tgaagcattc
    12421 gctgtgaatt tgactgattg gacctacgaa ttaattgaaa aaaaatatga gttttctggc
    12481 aaaaaagagc tttatttttc tggaagagtg atcgaaagtg ttcctgcacc ttctttagaa
    12541 gaaaatgtaa aagtgatcca aaaagtagcc aagcatcttg gaaaaaaatt tgagacagaa
    12601 gaatttgata tcatggctga tttgaaagaa gctgtctatg atacgataga agaaaaaggt
    12661 cggttggatc atgagatgat tgcggaaaaa gtcttcaagg aaaacatcac ggcaaaaatg
    12721 gcttttaaag aagaagtaca agagcaagga tttgtcccag aagcacctcc tgtaaaagaa
    12781 gtacaagaaa tttctgagaa aaaatttgga aaacaaaaat tcaagctatc aaacgggatc
    12841 gaactgatcg taccagtaga tgtatatcgc aacccagatt tgattgaatt tgtgaataat
    12901 ccggatggta cgatatccgt gatgatcaaa aatgtggatg aagtattgaa tcgtttataa
    12961 aaatgactag aatattaggt gttcaagtac cagtgatcag ttaaatgaat tgtgtcaaga
    13021 gaaggatcgg aaaagacgaa tataacaa
//
LOCUS       DACEMK010000078        13026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEMK010000078 DACEMK010000000
VERSION     DACEMK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.080"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(319..1038)
                     /locus_tag="IW715_001998"
     CDS             complement(319..1038)
                     /locus_tag="IW715_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1022599.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1087..2733)
                     /locus_tag="IW715_001999"
     CDS             complement(1087..2733)
                     /locus_tag="IW715_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1022600.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2912..3502
                     /locus_tag="IW715_002000"
     CDS             2912..3502
                     /locus_tag="IW715_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1022601.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3594..5099
                     /locus_tag="IW715_002001"
     CDS             3594..5099
                     /locus_tag="IW715_002001"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1022602.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5183..6535)
                     /gene="celB"
                     /locus_tag="IW715_002002"
     CDS             complement(5183..6535)
                     /gene="celB"
                     /locus_tag="IW715_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1022603.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNSSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6535..7053)
                     /locus_tag="IW715_002003"
     CDS             complement(6535..7053)
                     /locus_tag="IW715_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022604.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7069..7395)
                     /locus_tag="IW715_002004"
     CDS             complement(7069..7395)
                     /locus_tag="IW715_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1022605.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7408..9387)
                     /locus_tag="IW715_002005"
     CDS             complement(7408..9387)
                     /locus_tag="IW715_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1022606.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPSVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9408..9722)
                     /locus_tag="IW715_002006"
     CDS             complement(9408..9722)
                     /locus_tag="IW715_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1022607.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9890..10183
                     /locus_tag="IW715_002007"
     CDS             9890..10183
                     /locus_tag="IW715_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1022608.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10261..11373)
                     /locus_tag="IW715_002008"
     CDS             complement(10261..11373)
                     /locus_tag="IW715_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1022609.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11760..11939
                     /locus_tag="IW715_002009"
     CDS             11760..11939
                     /locus_tag="IW715_002009"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022610.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12133..12303
                     /locus_tag="IW715_002010"
     CDS             12133..12303
                     /locus_tag="IW715_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1022611.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12727..>13026)
                     /locus_tag="IW715_002011"
     CDS             complement(12727..>13026)
                     /locus_tag="IW715_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1022612.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4103 a         2621 c         2217 g         4085 t
ORIGIN      
        1 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaga
       61 ggttgtgaat tcagcgtttt ggcctgagta ttagggagat cgtgtcctta ctatctaact
      121 ctgagtcaca aggaacaatg gggactgtaa caaaagtctt gtcacagtcc cttattccga
      181 acaaacggtg ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaaagcca
      241 tccgaaagtc agacggacaa gtctgctttt cagatggcgc tacaactact ctgcttattc
      301 ttttagctat ccattcaatt atttttctct cacttgttct agctcttctt tggtcatggg
      361 tcgatattgc cctttattca aatctttagg cagccaaaat tttcccatac ggattctttt
      421 taaatataag acttcttttc ctactgcttt gaccattcgc ttaacttggt gaaactttcc
      481 ttcatgcaaa atcagacgga taattgaagt ttctgtctct tgatctactt gatcaatttg
      541 gagttttgcc ggcaatgtct gctcatcgcc atcaataatc agtccttctg caaataaacg
      601 gatatcttct tctgtcatta taccgctaac ctcagctaga tattctttct ccacatgttt
      661 tttaggtgat agtaactgat gggcaaacac cccgtcattc gttaaaatca agaggccctc
      721 tgtgtcttta tctaatcggc cgactggaaa aagatcttcc cgataatcct ggtcttccaa
      781 taaatcgatt acggtttgat catgattatc ctgagtagca gaaatgactc ctgcaggttt
      841 atgcaaaatg tagtaaaaat ctttttgata cgtaatgatt tctcctaaat aagtaaccgc
      901 atcttcgaat tcatttactt gcgctttatc gcttttcacc acttgtccat tgacttcgat
      961 cgttcctttt ttgattaatc ctttgacttc tttgcggctg ccaagtccca tatctgctaa
     1021 aaacttatct aaacgcatgc gtttccccca tttcaaaaga gcatcaatct agctcttctg
     1081 attcacttat tttattttta atttatgtcg taaacgcgct attcctggtc ctagtagtct
     1141 ttctgccaag cggattttca aagctaaata accatacact aaagccccta tacctgctac
     1201 tacaagaatc aacaatagag actggaattt gctatcttcg tttaaaaaag caccaaaaat
     1261 catctttgtg atccacgaag ccaaaagcat aatcaaggta agaataaaga tcagcaatgt
     1321 cctgcgccag acaaaacttg gtttaaagcg ggctacttca tggattctct ttaagatcaa
     1381 ggtacaagac acagcaaatc cgatcattgt cgccaataaa gggccgtata cttcaaataa
     1441 acgaatggct gggtattgaa gaaggagttt cactagaaac ccaataacca aataattaat
     1501 tgccgccttg ttttcaaaca ttccttgcag catgttggaa actagcatat atagtcctag
     1561 gaagagtcct acaaaacttg cttggatcag tacattactt cctagagaat ccggtgtata
     1621 aaataatgta tttagcggat aggcaagtaa cataacacca aatgtcgccg gaaacataaa
     1681 aaatgaaaac aactgtaaat ttccgcttgt caattttgcc agacctactc gatctttcaa
     1741 cgtaacagct tctgtaatca acggtaaccc agtagacgcg atcgatgttg ccaatgcaat
     1801 aactaccatc gtcaacttat ctggattggc actaaaaatc gaaaaaagat ccagtaactg
     1861 tgcattggaa tactcggtgg atgcagacat gatcttcata aaagtgaact gatcgactaa
     1921 tttgaagatc gttacccctg aacccacaat aataaacgga atcgcttctt taacggtatc
     1981 taacaacaat tccttcgttg caatatttat tttgttttcg ctgtagtgaa taaacgccga
     2041 tgtatacgct tgatgtttct tcaaaaaata caaaaggaca agaatacttg ctagcatacc
     2101 gataaatgcc gcgaaagttg attgcgtgac cgccgtcaca tagtcaccat ccatgacttt
     2161 catgatgatg aatgtagcca atagcatata aaaaatacgt gccacctgtt ctacaatctg
     2221 cgacagcgca taaggcatca tttcttgatt gccttgcaaa taccctcgta tcacactcat
     2281 acaaggaaaa accaatacag cgatactcaa agaacgcatc gttggaatca gttcttctcc
     2341 gccaccagaa gcatgagcta gccaaggaga ggcgatgtac atgaacaaag caaacaacag
     2401 gcctaacccg cccatcatct gtaatgcacg gataaataat cgtcgtgaag tgccatattc
     2461 attcaatgaa ttatagcgtg cagtttgttt tgcaatcgca gcaggtattc ctgctgtaga
     2521 gatcagtaga aacaatgcat atatattgta tcccatattg aataatccat tggcagcctt
     2581 agcattttcg cccatccaag cataccaggg aatgatatag acagcgccta gcaatcttga
     2641 aatgatatta ctggcggtca tccaagcaga gcctcgtgcc attttttctt gcggtgttaa
     2701 ttgatctaat gctggttttc tttgattatc catcggtcag attccaactt tctacaaaac
     2761 attactatca ttttaattgg taaaacagaa actggcaatc gattttatat aacttttata
     2821 agaacaattg tttttttgaa ttcggtttcg tttttttggt aaagtaaaga aatatacagc
     2881 cttttatggc taacatttat aggagatatc aatgacacaa gaaataaaaa aactacccgt
     2941 tatattttta ggtcttttct ttatcacagt tggattgaat tggttcttgc tccctcacga
     3001 tatagcttcc gccggagtgg gcgccatcgg acatttagtt gaaacggcct tttctatcca
     3061 acgctcagtt actgtttgga gcatcaatct tatgatgctg cttctagccg ctgtcctatt
     3121 aggaaaatca gtatttttta agactgtagc gggaagtctg ctttttcctg ttgttttaga
     3181 gattgttcca aaagtggaaa tgatctcctc acatttcttt tcactgattt caggaagtct
     3241 tttgttcagt cttggggttt acacattgta cacagtcggg gcctcaaatg gcggtataac
     3301 gataccgccg attattttcc acaaattttt tcggcttcca atgccgcaag gacttttact
     3361 taccaatagt cttatcgtct ttttgaatta ttatgttttt ggattgttgg agacactatt
     3421 cgttctccta tctattacag tcagttcatt gtttatgaaa atactcatcc gcttgtcccc
     3481 tgtgtcaaga aaatttgaat aaagctggat tttgtcatag cggaaacaaa cgatatatta
     3541 aaaagagtgt gctataataa accaagaaat tgaataaagc gagggatcaa tcaatgagct
     3601 tctctttaga agacatccgt catttactat taaaagaaaa tctattaaaa gaatttgttt
     3661 caacaagagg ctggcatctt gatgtaccag aaaaagctga atttcttcag ttaagttatg
     3721 attcaagaaa agctgatgct tccactttgt ttttctgtaa aggaatgaat ttcaaagaag
     3781 aatatttgga ttcagcaatc gaacagggta ttcaatatta tgtatcagaa caaccatacg
     3841 aaaattcagc agtaggaatc atcgtaacgg atatccgtaa agcaatggcg cttcttgcta
     3901 tggcttttta cgactatccg caaaataaat tgaaagtgat cggattcact ggaacaaaag
     3961 gtaaaacaac tgctgcgtac tttacaaaag caatcttgga tcacacaaca aataaaaaaa
     4021 cagcattgct ttctactatg aatacaacgt tagatggcaa aacatatttc aaatcccatt
     4081 tgacaacgcc tgagtcatta gatttatatc gtatgatgtc agaagcagtt gaaaatggta
     4141 tgactcatct agtgatggaa gtttcttcac aagcttacaa aacacaacgc gtatacggct
     4201 tgactttaga tgtcggaatc ttcttgaata tctcgcctga ccatatcagt ccgatcgaac
     4261 atccgacatt tgatgactat ttctattgca agcgacaatt gatcttgaat tcaaaaacag
     4321 ttgttttaaa tcatgaaagt gactattttg atttacttag ggaaacagcc gatctattcc
     4381 agattccaac gatcacttat ggtcgttcgg aagaaagcaa ttatcaagtg atccgttcag
     4441 ataaaggtac tcacggtttt actttatctt ctcatgaaga tcgcttagct atctgcgata
     4501 ctgcttatga tattttgctg gctggaggat tcaaccaaga aaatgcagcc agtgcgatca
     4561 ttgccgcagc actaacaggt gcctccagtc aagatgcaca agacggatta aaagaagctc
     4621 gtgtaccagg ccgaatggat caattgatcc aatccaatgg agcgcatgtc tatgtggact
     4681 atgcacataa ttatctaagc ttgaaaacat tgctgaaatt tgccaaaaat gaacatccag
     4741 atggccgtgt aattgttgta ttaggcagcc caggaaacaa agcaatctcc cgtcgacatg
     4801 attttggaaa agtgctatca gaaacagctg acatcgcctt tttgacggca gatgacccag
     4861 catttgaaga tccgaaaaaa atcgcggaag aaatcaatga agcaattact aatccagatt
     4921 tgatcgttca ttatgaaatg gatcgtcctg aagcgattcg acgcgcttta gcagaaagcg
     4981 ctccgcaaga ttcagtcgtt attgcgggaa aaggcgtgga tccctatcaa aaaatcaatg
     5041 gtgtagatga accttatgaa ggggattatg caatcgtgaa gcggttgatt gaagaataat
     5101 ggtatttata tactagtggt ttcccatttc taagaaaaaa agggactgtg attttttgtc
     5161 acagttcctt ttttcttaga aatcatttta acttttcact agctaattct gctgcctttt
     5221 ctctttcttc atttaataat ttattatcat agaatttgat aaatgggaac cagataatga
     5281 atgcgatgat tgcacaaatg actgctaaca cagcagcttt ccaatcacca ccactaccaa
     5341 caaatgctcc gatcccgact ggtgaaggcc acggcatctg tgcaagcatc ggtttgacga
     5401 tttccagccg aatggcaaaa taagcgagtg tagcagaagc catcggagct aagaaaaacg
     5461 gaaccgctaa atagggattg tagacgattg gcatcccgaa aatgattggt tcattgatat
     5521 tgaataatcc tggtacaagt gaggcttttc ctaacacttt cagctgatca gatttcgcga
     5581 ggaatgcaat aaagatgatc aatcctaaag tcgcacctga tccaccaacg gttacataag
     5641 aactgttgaa ttctcctgcc aacggataat ttgcgccttg tgcgttagct gccatattag
     5701 ctaatacgat cggggtcaag aaagaagtga tgatattcgc tccatggatc cctacgatcc
     5761 acaatgcatg gatcaagaaa taaatcacca tgacaccaag ccatgtattc gtcaactgcg
     5821 taacaaaccc aaatgggata gcaatcattt tgaagatatc cgttcctagt gcaactaata
     5881 atccattgat gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga gcagtgaaag
     5941 aacgagaaac tcctggcgga acaacatctg gcatttttat gacccaattt ctcttgacac
     6001 acatacaata caattcgacc gcaataatcg acataataat agctgtaaag attcctgaag
     6061 ttcctaaacg ttcgacaaaa gagcccatcc taataccatt tactacagtt tctgtctcat
     6121 tgatagaagc aatcaagctg attttaccat cagtcatgat caattcaggg atacacataa
     6181 agaatgcaaa aacagatagt aatgcccccg tgagcggatt aacatttaat ttttgttcat
     6241 ctcgttcaat tgacgttaat tcataagcaa acacaatggc aaagtaaata gataaaatcc
     6301 ccatcgtcat tgtattgacg atcatgtaca agtcactaac tttgaaaaaa gttgcattaa
     6361 aaaatccttc taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa aacatcgagc
     6421 ctacaatcgt gaaaggaata gaagccatcc ccgctgccat gacagcccga acgattttaa
     6481 gttgcgcaat tttccccata ggtcccatca ggtatttctg caaaaaatca aacattagtt
     6541 attctccttt cctaaatata acgctttttc atattgttta atacggatat actgttggtc
     6601 ttggttgagt gaaattttct ttaaacgata ctgtataaat gcactggcaa aaataccgct
     6661 tacaacaaga atgaaaacaa ataactgtcc tccattgtta tttgccataa aaggatacaa
     6721 gaatgtaaat cctgaggtaa acaacactgg aatcactaat aaattaaaga cactctggaa
     6781 caaaaaatac cactttgtcc atggaacgtt cgtttggtgt tttcgataaa gctttacttg
     6841 ttctcctact gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac ttcctaaact
     6901 tttagctgat agaagcagaa tcatccattg catgttcatg aaaaagaaaa aggcagtcag
     6961 gtaacgtacg attagataac gattgaaata catgtttttc aaactaagtt gctttttttc
     7021 taattcgatt ttccttttta ctgtatcctg cacaatgatc cttcctttct atttattttt
     7081 ttgatacaaa taattcattt cctgtgcaac ttccaataat gtcatcgtcg tcatcagatg
     7141 atcttgtgca tggaccatga taatttccat ttctattttt gtcccgctgg catattcttt
     7201 cagaagttga gtttgtgact gatgcgcatg aagaatctct tcatttgctt gtcgcaactt
     7261 ctcttctact tcttcaaaca tctctttacg catcgagcga aatgcttcat gtatcagtgt
     7321 ccgtgcattt ccgctatgga aaatgatatc aaaagaagtc acttgtagtg tttcactgtt
     7381 tattttttca gccatctgtt ttcctcctta ttcgatcagt ttgataaatt tactgctgaa
     7441 attctcaaaa ataggttcag ctaagatttc ctgctgtact tttagctggt caactaacct
     7501 aacgattgct ttagtcacga ccgtaattcc ttcattttcg acataagaag gcgaaactaa
     7561 aaaaacaaaa tgaatttctt gatattgatc ccactgcatc ccagaaggaa tcaatgcaac
     7621 ggctaccttt gtttcgattc caactggaac agagggatgt gggactgcca ctgtatcact
     7681 gaatatgatt tgtcccatat tttcacgttg ctggatctgc tttcgcattt ctcttatata
     7741 tccttcttct tcatttatgc tcagcttttg cagcaaatca tgaagaattt cttccttttt
     7801 cggttcgtac gtataagtct tgaaaaattt ttcagacaac tgttcggtgt aaatctgctt
     7861 tttttgctgt agagaaaatg ctggtatcgg tctatctgag gaatgatagg gtatagattc
     7921 tccaactacc tttcgtattt tacgaacatc atcttcattc aaaaaaatac tgacatgtaa
     7981 aactggaatt ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca ggtcgattcc
     8041 ttttaaagtt gtttcattga tctcataata gccttttaca cttaccactg taatattttt
     8101 accgaattca ctgactacgc gattctttaa tagttgggca ctgccgtaac ccgttgcaca
     8161 gataatcaat gcatgtagtt tatgcgcagc tctttctttt tccaatgccg ccatgagatg
     8221 caaagccaaa taggcccatt catcttcatt gatggtatag tttttcaaag ccggcatgtt
     8281 gctgaaatgc ctttttactg cgtcaaagac tttgggattt tctttcttta tctcctcagt
     8341 caatgggttc tcaatgcgaa tacctcggct caatcgaatc atcatcggtg acaaatgatc
     8401 gagtagtcca ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt cctgagacaa
     8461 tcttttcaga actttcgcta attcctttga tagttcctga tcattttgat tttctttgtg
     8521 attcgactta gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg gaaacgatac
     8581 acctgcgatt acctcaatac gttgaacgat ccgctgcgcc acttgatact ctactcgctt
     8641 agaaacttct tgttcgatgc ctaattcctt gatttcaaac ccttctcgga gccgtttgat
     8701 ccccaaagcc aagtgtaaag tcagattctg tatgataaaa tccgaaactt ttaacttagc
     8761 ttctcttgtt tcatcgagaa tgataatgac tagctcttca aaactgatgt ctgggaaaaa
     8821 acggctttgc cctaaatatt ctttcaatga atttccataa tggtttccaa aaaaagtatc
     8881 catgataaaa tgccgtcgat ttctttcttc cccttcgatc catgtccctt gaccatgctt
     8941 agaaacgatt tttagtgaat atggaatcaa tcgctccttg atttcttgca tgtcctttgt
     9001 caaactggaa cgactgatat aaagctcttc tgccaaatca tccataaaaa gaatggcatc
     9061 ttccaaaagc aattggttca atatatactt ttgtcgatct tcggactcat aaaatccttg
     9121 cggttctcta gcatatccag gtactatttc ttgccgactc aaaaacaagt caaatgtcag
     9181 tctttgtaaa atatgcagtt ggtagccata acctggttta gcaatgatct tacctccgtt
     9241 tttttctact aactctttga tccgctgcaa gtaattacgg attgtccgat cagataataa
     9301 caattcagaa gctaaacgtt gactggtgac atactccttc ttgtgatcaa tcaaatatct
     9361 caacagcaat ttttccttct gtttcattat catccactcc tattccatca tatgttctct
     9421 tcgatcacct ctgctaactt ctggatgcct gtagggattg ggacatacgc ttggaaaggg
     9481 atactaacaa caggctttcc aacagattct cctaattttt tgaatttatc caaaaacatc
     9541 gtcgtttgcg ggctgatcag aaataaatca aaatcactat tcttaatcat attatcgcct
     9601 tctgtagctg aaactgcatc tacgagtacc tcttctcttt tttgcttaaa gaaatccgtt
     9661 gtctttgagg ccatcatcga ggatgacatt cctgccgcac aaataatcaa tgcttttttc
     9721 ataaatcaat cactcctttt atttttataa accaagcata tcttgtaagc gttttctatt
     9781 caaaatatat atttccgtag cattgcggaa aaaatgtgta tcatatcttg aataataatt
     9841 tctggcaccg tgatatacta gtgatagaat tttttcagga ggggatttag tgagtatcac
     9901 agtgaatatt ttgtatacaa gtacaaatgg acaagccaga aaatttgcag aagaaatggt
     9961 cgaaagaaac attgtcaaag cgattcgtgc agaagaagga aacgagaaat atgactatta
    10021 ctttccacta gaagatccag aatctctttt actgatcgac cgttggaaag atgaagaagc
    10081 aatcgaaaag catcacaaat ccgaaatgat ggctcaaata aacgaactcc gtaaaaagta
    10141 taaattaaaa atgaaagtcg aacgtttcac tgatttaccg taaaaagata cacatggaat
    10201 gatagcaatt tatcatccat gtgtatcttc actttttcct tattttttat tcgatctcat
    10261 ctaatgattc aaaccgtgtt tttctcttat ttttcggata agtatttcag tcaccccaat
    10321 atacatgatt cctaaacata ccccaaggat attctcaaaa atgcggatcg ttactgctcc
    10381 tggaaccccg aataaactgg cagtgattgc caaagcacca aaagaattaa aaactgtttt
    10441 atatctatag gtagaacaaa cacctaaagc tagaccacct aatataccga cccagttaaa
    10501 aggaataaat tggctgatac caataaataa aacacatcca atcaatgagc caatgattcg
    10561 gtctactgct cgttctttga aaccaatcga catcaatcca tagctagata caatagaaga
    10621 aaatgcaaac cctgcccaca tcaatcgctg aaaagggaca tactctcctg caacgatcaa
    10681 aagactaatg ccaaatgcat agatgagcat ccaaattttt tcctgcgaat acatgccttt
    10741 caaaaaaatc tctttaaaca agcttttgtc tttgttcttt tctcgatgtt ttctgtaaag
    10801 gagaactgaa aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg taaagaaatt
    10861 cttgttcaac gaatctttag gtaacgaata aacaataaag agatatgaaa agccgtacaa
    10921 agaagcattc cccattttgg gatcactagc ggttgccatt aatatgctgc ttagtaaaat
    10981 aaaatgaatc aacatgccca taactgacca tttgatcagc tggacatacg gtgctactaa
    11041 tagtgagaac atgacgattg ctaaacctat gatcgaatgg gaaatcttat aaccaaagtc
    11101 aacaaaacga atactcatca acatacagaa taaaaccaca gcgtatggtt tatgcttcga
    11161 ttcaaataat gttgtaatta taatgataaa acaaatggca aacaatacga tgaataatga
    11221 tcgtaaaaac aatgaacaaa tatacatgca cttttctttt ttagaagctg cttctcgtat
    11281 tttttgtttt aaaataaacg gatctagttg tagagactga taaaaatcca cctgttgtcg
    11341 tcctttcttt ttacttttat tttttacagt catctatgac aggttgtaaa tactatggaa
    11401 catcatatgg cgttgtcatt ttcttgtcaa ctatttttcc tattcatgtt tttataaaaa
    11461 aacatatcgt tttcttgtat gattaaatca ccacaaaata aacaactgag agaacgatat
    11521 gctttatgaa tggttctatc aaaaaaattc tgagatcaat agacaaaaat ttgatgatta
    11581 cagaggtctc ttacgaaacc ttacagaaga aaaaaacatt gatcgttgct gcagttctct
    11641 tccctgctcc atgtgtctgt agaaattgtg gttctgctgt ggtagatgga aataggaaat
    11701 taatggtggt gaaaaacaag aagaaggaaa ccattgtcca ctttgaataa tataatcata
    11761 tgccaatggt tatacgccta aaaaagcagc gctatacctg taaaaactgt cgaagccatt
    11821 ggaacgctca aagttatttt atccgtccaa ggcactcgat ttcaaatcat gtcagacata
    11881 aaatcacttc tttactgact gaaaaagtgt cgttattttt tatttcgaaa agttgttagg
    11941 tatctttgac aaccgttatt cgcacattga aagagttgag aagctattta ccaaagtctt
    12001 ccaaaagaat tctgtcttga gtgttgaggg ttgtgaattt cgttctcacg tttccgttga
    12061 ggataaactg agttttattt ccgcggatgg cgagacagaa aaattaatcg agaaaattta
    12121 cctacacaaa aaattcctcg attaacaaac tatttcctta gctgcactaa tcctaaagaa
    12181 atagaattct tagttacaga catgaacgcc acctactttc agctcaccaa acatgttcta
    12241 ccaaatgcga aattggtgat tgatcgatta cttggttttt ccacgccttt caaggaggcg
    12301 taacctttat tcacgagttg gttgaagctt tccgagacaa aagacccgac ttgtttttct
    12361 ccttattgac aaagagccat taaaactaga gaaaaaatta caactgatcg ctgattattt
    12421 gtagaaagaa aatcaaagta ttacccaata aacttgagtc attaggcaaa aaacactgcg
    12481 aaactcaaga atgattttcg cagtgttttt gtatattttt tattaactag tattactaca
    12541 aaggtttatt tttcacttct caacgcagca caaaattgtc tttacttatt tactggttgg
    12601 gttcaacctt ctctactccg atactaatat ttgtttgtat accccttcta aaatcggtag
    12661 tttccattca attgatgggt actagttgat tttaaaaact atgcttattc aattttttat
    12721 tttagctcat atatttcgtt tctttctaaa ttttatactg atgaaaacaa gtagcacacc
    12781 caatattgac aatataaact ggctaatgat ggtttctcca gtttttggaa gatgcttatt
    12841 tccactgctt gttggattgt ttcctcttat atctgagcta tcagcaactt ttctattctc
    12901 ttttgttaaa aagatcgcca cttcttgttc tttttcgatc ttgaaagtta ctggtgtcgc
    12961 atccaattca taccccgttg gcgcttgagt ttctaccagc tgatatgctc ctggtgctaa
    13021 gccatc
//
LOCUS       DACEMK010000079        13006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEMK010000079 DACEMK010000000
VERSION     DACEMK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.081"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW715_002012"
     CDS             complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW715_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1022613.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1258..3099)
                     /locus_tag="IW715_002013"
     CDS             complement(1258..3099)
                     /locus_tag="IW715_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1022614.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3281..5674)
                     /locus_tag="IW715_002014"
     CDS             complement(3281..5674)
                     /locus_tag="IW715_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1022615.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5781..6641)
                     /locus_tag="IW715_002015"
     CDS             complement(5781..6641)
                     /locus_tag="IW715_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1022616.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6700..7221)
                     /locus_tag="IW715_002016"
     CDS             complement(6700..7221)
                     /locus_tag="IW715_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1022617.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7541..8260
                     /locus_tag="IW715_002017"
     CDS             7541..8260
                     /locus_tag="IW715_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1022618.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8286..10877
                     /gene="mprF"
                     /locus_tag="IW715_002018"
     CDS             8286..10877
                     /gene="mprF"
                     /locus_tag="IW715_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1022619.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW715_002019"
     CDS             complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW715_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1022620.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12620..12976
                     /locus_tag="IW715_002020"
     CDS             12620..12976
                     /locus_tag="IW715_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1022621.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3738 a         2718 c         2310 g         4240 t
ORIGIN      
        1 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
       61 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
      121 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      181 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      241 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      301 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      361 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      421 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      481 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      541 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      601 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      661 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      721 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      781 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      841 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      901 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      961 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     1021 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1081 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1141 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1201 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1261 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1321 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1381 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1441 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1501 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1561 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1621 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1681 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1741 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1801 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1861 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1921 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1981 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     2041 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2101 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2161 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2221 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2281 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2341 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2401 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2461 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2521 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2581 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2641 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2701 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2761 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2821 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2881 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2941 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     3001 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3061 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3121 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3181 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3241 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3301 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3361 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3421 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3481 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3541 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3601 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3661 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3721 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3781 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3841 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3901 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3961 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     4021 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4081 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4141 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4201 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4261 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4321 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4381 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4441 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4501 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4561 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4621 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4681 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4741 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4801 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4861 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4921 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4981 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     5041 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5101 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5161 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5221 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5281 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5341 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5401 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5461 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5521 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5581 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5641 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5701 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5761 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5821 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5881 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5941 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     6001 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6061 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6121 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6181 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6241 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6301 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6361 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6421 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6481 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6541 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6601 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6661 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6721 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6781 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6841 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6901 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6961 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     7021 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7081 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7141 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7201 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
     7261 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7321 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7381 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7441 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7501 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7561 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7621 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7681 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7741 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7801 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7861 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7921 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7981 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     8041 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8101 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8161 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8221 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8281 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8341 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8401 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8461 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8521 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
     8581 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
     8641 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
     8701 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
     8761 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
     8821 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
     8881 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
     8941 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
     9001 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
     9061 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
     9121 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
     9181 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
     9241 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
     9301 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
     9361 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
     9421 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
     9481 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
     9541 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
     9601 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
     9661 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
     9721 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
     9781 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
     9841 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
     9901 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
     9961 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    10021 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    10081 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    10141 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    10201 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    10261 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    10321 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    10381 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    10441 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    10501 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    10561 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    10621 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    10681 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    10741 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    10801 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    10861 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    10921 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    10981 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    11041 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    11101 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    11161 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    11221 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    11281 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    11341 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    11401 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    11461 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    11521 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    11581 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    11641 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    11701 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    11761 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    11821 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    11881 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    11941 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    12001 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    12061 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    12121 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    12181 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    12241 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    12301 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    12361 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    12421 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    12481 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    12541 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    12601 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    12661 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    12721 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    12781 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    12841 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    12901 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    12961 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaa
//
LOCUS       DACEMK010000080        12962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEMK010000080 DACEMK010000000
VERSION     DACEMK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.082"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..149)
                     /locus_tag="IW715_002021"
     tRNA            complement(77..149)
                     /locus_tag="IW715_002021"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IW715_002022"
     CDS             530..1099
                     /locus_tag="IW715_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1022622.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IW715_002023"
     CDS             1086..1946
                     /locus_tag="IW715_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1022623.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IW715_002024"
     CDS             2136..2840
                     /locus_tag="IW715_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1022624.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IW715_002025"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IW715_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1022625.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IW715_002026"
     CDS             4677..5993
                     /locus_tag="IW715_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022626.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IW715_002027"
     CDS             5994..6863
                     /locus_tag="IW715_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022627.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IW715_002028"
     CDS             6918..7727
                     /locus_tag="IW715_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1022628.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IW715_002029"
     CDS             complement(7830..8459)
                     /locus_tag="IW715_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ1022629.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IW715_002030"
     CDS             8629..9921
                     /locus_tag="IW715_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1022630.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IW715_002031"
     CDS             complement(9997..10659)
                     /locus_tag="IW715_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1022631.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IW715_002032"
     CDS             10884..11168
                     /locus_tag="IW715_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1022632.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IW715_002033"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IW715_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1022633.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4321 a         2240 c         2804 g         3597 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aa
//
LOCUS       DACEMK010000081        12673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEMK010000081 DACEMK010000000
VERSION     DACEMK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.083"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(147..1178)
                     /gene="queA"
                     /locus_tag="IW715_002034"
     CDS             complement(147..1178)
                     /gene="queA"
                     /locus_tag="IW715_002034"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1022634.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1371..2546
                     /locus_tag="IW715_002035"
     CDS             1371..2546
                     /locus_tag="IW715_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1022635.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2550..3191
                     /locus_tag="IW715_002036"
     CDS             2550..3191
                     /locus_tag="IW715_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1022636.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3188..4105
                     /locus_tag="IW715_002037"
     CDS             3188..4105
                     /locus_tag="IW715_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1022637.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4102..4773
                     /locus_tag="IW715_002038"
     CDS             4102..4773
                     /locus_tag="IW715_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1022638.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4969..5803)
                     /locus_tag="IW715_002039"
                     /pseudo
     CDS             complement(4969..5803)
                     /locus_tag="IW715_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6004..6270)
                     /locus_tag="IW715_002040"
     CDS             complement(6004..6270)
                     /locus_tag="IW715_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1022639.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6270..6533)
                     /locus_tag="IW715_002041"
     CDS             complement(6270..6533)
                     /locus_tag="IW715_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1022640.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7008..7193
                     /locus_tag="IW715_002042"
     CDS             7008..7193
                     /locus_tag="IW715_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1022641.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7335..9158)
                     /locus_tag="IW715_002043"
     CDS             complement(7335..9158)
                     /locus_tag="IW715_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1022642.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9755..12460)
                     /locus_tag="IW715_002044"
     CDS             complement(9755..12460)
                     /locus_tag="IW715_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1022643.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3651 a         2637 c         2389 g         3996 t
ORIGIN      
        1 ttcaaggtgt cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa
       61 catcaaaaag tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
      121 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
      181 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
      241 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
      301 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
      361 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
      421 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc
      481 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
      541 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
      601 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
      661 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
      721 ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca
      781 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca
      841 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc
      901 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
      961 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     1021 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga
     1081 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct
     1141 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     1201 gtgcttcatc attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc
     1261 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     1321 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     1381 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     1441 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     1501 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     1561 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     1621 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt
     1681 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     1741 tgccccgtga gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta
     1801 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     1861 gtgcgcttga tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc
     1921 gcttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa
     1981 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct
     2041 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     2101 ccaagccaaa tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac
     2161 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     2221 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     2281 agcgaggaaa agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag
     2341 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc
     2401 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     2461 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata
     2521 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     2581 aaattctctc aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta
     2641 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     2701 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     2761 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     2821 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     2881 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     2941 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     3001 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     3061 attaccaacc agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg
     3121 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     3181 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     3241 gagttgttct ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     3301 tggaatcact tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta
     3361 tacagataaa aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc
     3421 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     3481 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     3541 atttgagaaa agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     3601 ctttcttgtt cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa
     3661 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     3721 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     3781 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     3841 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     3901 cccaccatat gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt
     3961 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     4021 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     4081 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     4141 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     4201 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     4261 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     4321 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     4381 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     4441 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     4501 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     4561 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     4621 ggagatatca ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt
     4681 gcattgccaa ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga
     4741 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     4801 cgaccatttg ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca
     4861 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
     4921 tatactccaa cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt
     4981 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
     5041 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
     5101 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
     5161 acatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
     5221 aacttgccat cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg
     5281 gaggtttgcg gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag
     5341 acttggcggt acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
     5401 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
     5461 tgatactgct attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat
     5521 tcatgttcgg caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt
     5581 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
     5641 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
     5701 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
     5761 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
     5821 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
     5881 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
     5941 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
     6001 acttcactca taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac
     6061 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
     6121 tctcagcttc tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
     6181 ttcttcaaat tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga
     6241 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
     6301 ttcgtaaagc cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
     6361 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg
     6421 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
     6481 aactgataaa aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct
     6541 cctccttttt ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa
     6601 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     6661 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     6721 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     6781 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     6841 acctgaggat acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     6901 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg
     6961 atttgatact tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt
     7021 atggtaacaa aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc
     7081 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     7141 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     7201 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     7261 tgggaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     7321 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     7381 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     7441 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     7501 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     7561 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     7621 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     7681 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     7741 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     7801 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     7861 aaaggccaaa tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg
     7921 aacgcacctg taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat
     7981 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     8041 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     8101 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     8161 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     8221 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     8281 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     8341 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     8401 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     8461 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     8521 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     8581 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     8641 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     8701 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     8761 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     8821 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     8881 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     8941 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     9001 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     9061 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     9121 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     9181 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     9241 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     9301 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     9361 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     9421 atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga
     9481 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     9541 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     9601 tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     9661 gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
     9721 tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
     9781 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
     9841 ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt
     9901 aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
     9961 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    10021 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    10081 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    10141 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    10201 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    10261 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    10321 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    10381 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    10441 ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    10501 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    10561 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    10621 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    10681 ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat
    10741 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    10801 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    10861 cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg
    10921 ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac
    10981 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    11041 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    11101 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    11161 ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    11221 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    11281 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    11341 ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt
    11401 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    11461 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga
    11521 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    11581 caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt
    11641 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    11701 caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt
    11761 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    11821 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga
    11881 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    11941 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    12001 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    12061 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    12121 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    12181 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    12241 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    12301 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    12361 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    12421 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    12481 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    12541 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    12601 ataattaagg attacagact ttactttatc atgaaataaa agggaaagat aattttatgc
    12661 caaagttttt atc
//
LOCUS       DACEMK010000082        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEMK010000082 DACEMK010000000
VERSION     DACEMK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.084"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW715_002045"
     CDS             6..305
                     /locus_tag="IW715_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1022644.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW715_002046"
     CDS             378..1739
                     /locus_tag="IW715_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1022645.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW715_002047"
     CDS             1761..2021
                     /locus_tag="IW715_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022646.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW715_002048"
     CDS             2035..2787
                     /locus_tag="IW715_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1022647.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW715_002049"
     CDS             complement(2881..3486)
                     /locus_tag="IW715_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1022648.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW715_002050"
     CDS             complement(3539..4513)
                     /locus_tag="IW715_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1022649.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW715_002051"
     CDS             complement(4891..5670)
                     /locus_tag="IW715_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1022650.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW715_002052"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW715_002052"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1022651.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW715_002053"
     CDS             7161..8558
                     /locus_tag="IW715_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1022652.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW715_002054"
     CDS             8639..9079
                     /locus_tag="IW715_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1022653.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW715_002055"
     CDS             9227..9553
                     /locus_tag="IW715_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1022654.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW715_002056"
     CDS             9554..10303
                     /locus_tag="IW715_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022655.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW715_002057"
     CDS             complement(10383..10838)
                     /locus_tag="IW715_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1022656.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IW715_002058"
     CDS             complement(11265..11540)
                     /locus_tag="IW715_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022657.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IW715_002059"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IW715_002059"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1022658.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2176 c         2586 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACEMK010000083        12295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEMK010000083 DACEMK010000000
VERSION     DACEMK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.085"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IW715_002060"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IW715_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1022659.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IW715_002061"
     CDS             complement(740..1462)
                     /locus_tag="IW715_002061"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1022660.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IW715_002062"
     CDS             complement(1598..2479)
                     /locus_tag="IW715_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1022661.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IW715_002063"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IW715_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1022662.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IW715_002064"
     CDS             3719..4198
                     /locus_tag="IW715_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1022663.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IW715_002065"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IW715_002065"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1022664.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="IW715_002066"
     CDS             5499..5627
                     /locus_tag="IW715_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1022665.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="IW715_002067"
     CDS             5631..6176
                     /locus_tag="IW715_002067"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1022666.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IW715_002068"
     CDS             complement(6629..8320)
                     /locus_tag="IW715_002068"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1022667.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IW715_002069"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IW715_002069"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1022668.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IW715_002070"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IW715_002070"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1022669.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IW715_002071"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IW715_002071"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1022670.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3373 a         2542 c         2210 g         4170 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttatt
//
LOCUS       DACEMK010000084        12215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEMK010000084 DACEMK010000000
VERSION     DACEMK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.086"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            592..1920
                     /locus_tag="IW715_002072"
     CDS             592..1920
                     /locus_tag="IW715_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1022671.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IW715_002073"
     CDS             2122..2955
                     /locus_tag="IW715_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1022672.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IW715_002074"
     CDS             2971..3708
                     /locus_tag="IW715_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1022673.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IW715_002075"
     CDS             complement(3863..4831)
                     /locus_tag="IW715_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1022674.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IW715_002076"
     CDS             5063..5896
                     /locus_tag="IW715_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1022675.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IW715_002077"
     CDS             5930..6769
                     /locus_tag="IW715_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1022676.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IW715_002078"
     CDS             6795..7355
                     /locus_tag="IW715_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1022677.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IW715_002079"
     CDS             7554..9275
                     /locus_tag="IW715_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1022678.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IW715_002080"
     CDS             9440..10582
                     /locus_tag="IW715_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1022679.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IW715_002081"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IW715_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1022680.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4170 a         2053 c         2533 g         3459 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cagaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt gcgaaaaagc
    12181 tgccctgcat caagtaataa gaacagccaa acaaa
//
LOCUS       DACEMK010000085        11915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEMK010000085 DACEMK010000000
VERSION     DACEMK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.087"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..927
                     /locus_tag="IW715_002082"
     CDS             <1..927
                     /locus_tag="IW715_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1022681.1"
                     /translation="IFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNK
                     WFRLLNIPVIIHLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIE
                     PNCKTVVVENSIVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKK
                     FIFEIAGAGDEEISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGL
                     PISILEAISYGMPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVK
                     FSRCSKDLAITKFNDGNFFDRILNLYQKGSKE"
     gene            1495..2556
                     /locus_tag="IW715_002083"
     CDS             1495..2556
                     /locus_tag="IW715_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1022682.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            2874..3464
                     /locus_tag="IW715_002084"
     CDS             2874..3464
                     /locus_tag="IW715_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAQ1022683.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            3491..4801
                     /locus_tag="IW715_002085"
     CDS             3491..4801
                     /locus_tag="IW715_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1022684.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            4798..5516
                     /locus_tag="IW715_002086"
                     /pseudo
     CDS             4798..5516
                     /locus_tag="IW715_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            5531..6616
                     /locus_tag="IW715_002087"
     CDS             5531..6616
                     /locus_tag="IW715_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1022685.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            6752..8284
                     /locus_tag="IW715_002088"
     CDS             6752..8284
                     /locus_tag="IW715_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1022686.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            8462..8683
                     /locus_tag="IW715_002089"
     CDS             8462..8683
                     /locus_tag="IW715_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1022687.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            9265..9882
                     /locus_tag="IW715_002090"
     CDS             9265..9882
                     /locus_tag="IW715_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1022688.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            9879..10409
                     /locus_tag="IW715_002091"
     CDS             9879..10409
                     /locus_tag="IW715_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAQ1022689.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(11275..>11915)
                     /locus_tag="IW715_002092"
     CDS             complement(11275..>11915)
                     /locus_tag="IW715_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ1022690.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         4250 a         1311 c         2294 g         4060 t
ORIGIN      
        1 atatttaaat ctttatattt tatgtatgca tttgctaaaa ttataggtat ctctttattg
       61 agtaaaccta atattattca tatgcacatg tcgtacagag ggagttttag taggaaatat
      121 gtgctgaata agtggtttag attattaaat attcctgtaa tcatccatct tcatggatcg
      181 gaatttaaaa aatggtatga tagtgtttct gtaaaaaaac aaaatcagat tcgcttgctg
      241 ttaagagagt ctgattccac gattgtactt ggagaaaaat ggaagaagat aattaaagaa
      301 attgagccaa attgtaaaac tgttgttgta gaaaattcta ttgtagttcc agaatatacc
      361 ataaagtgga attcaaaaat gtgccgattt ctgtttatgg gagttttgat aaagcgaaaa
      421 ggtgtacacg atttggtaga agcagttaaa aacattgcag aacaatatcc agaaaaaaag
      481 tttatttttg agattgctgg agcaggtgat gaagaaatat cgctcaaaga attgatagaa
      541 gaatataatt tgcaatcgta ttttgaattt acaggttggg ttgtgggaga aaaaaaggag
      601 gagtgctata gaagatcaaa tgtgatgatt ttaccgtcct ataacgaggg attgcctata
      661 tctattctag aggcgatttc ttatggaatg cctgtaattg ctacagatgt aggagatatt
      721 tcttcagcag tacgtgatgg agaaaatgga tatttgtttc aacctggtga cgtatcagcg
      781 ttgacatctg caatactaaa aatcaatgat aaagataaat acgtaaaatt ttcacgttgt
      841 tcgaaggatt tggcaataac gaaatttaac gatggaaatt tctttgatag aattttaaat
      901 ctgtatcaaa aagggagcaa ggagtgatga caaggaaaat aaaaaatgtt tcgattatgt
      961 aatgaactta ttaagtgaat aaaatagaga gaaaaaatat tgaaatagac gatgtaaatt
     1021 tttatccgtg taagaattca aatgaatagt agtgttttag tgcttgtaat agagcaagag
     1081 tacaagcacg gtacattata taaaaacatt aatgatattt ctataaataa atagattttg
     1141 tattctggat gatgaagagg aagatattat ctgataactt accctaaact ttgcaggggg
     1201 tggttataag ttgtgtatat gtggcagtaa tgaatgagtg aaataatcat attaagaaat
     1261 aaaattgcaa tattgtgtaa tacaaaagta tattttcatt attccaaggg aaagcatact
     1321 tacgaggatc atattattga tttaataaat aatttaaagg aaataatttt tctagaagaa
     1381 gatagtatgt cattcacatc atcaagatca gagtctattt ttttcagaga atttaagaaa
     1441 atcgttgatg ccgtcattga ctaattgaga aaacgtgagt gaaaggatag aaggatgaat
     1501 tcgagtgatc taattagtgt aatcatacct gtatataatg ttgaaaagta tgtaggaagt
     1561 tgtttggaat cagtgtgtaa tcaaacatat aagaacctag atattataat tgtggatgat
     1621 ggatctaagg attcatctaa taaaatatgt gaagactatg cattaaagga ttcaaggatt
     1681 cgtattattc ataaaaataa tgctggcttg ggattggcaa gaaatacagg acttgaacat
     1741 gtaaaaggaa aatatgttgc ttttattgat tctgatgatt ttgttgcagc taattatatt
     1801 gaagcaatgt taaatggtat aaaaaggagt aatgcagaca catgttatgc tggatattat
     1861 gaatattatg atgataataa tattatttca aagccagcaa gatatgatag tacgatattt
     1921 acaaataacg ctataattga aaaagtgttg cttggaatga ttggcactga gccgagttat
     1981 aaagtcgatt ttatattgcc aatgtcagtt tggcatgcgc tgtattcatc tgagataatt
     2041 caacagcaca atatcaagtt tccgtcagaa agggattata tatctgaaga tatcatattc
     2101 catatttcat atttgcaaca ttcaaaaata acaagtttta ttaaagattg tatctattat
     2161 tatagatgta ataataataa ttcattgaca cataatttta gagataatga gttcgattgt
     2221 aatattgaat tagttaaaaa aattgaaggc gatttaagct atatattaga aaatgagata
     2281 tttgtaaaca aaacaaggag acttttgttg ggacgctgtc gtacttgtat tcaaaaggca
     2341 gttagatatg ctagttttaa taaagaattt gatttaaact cctatttgtc taagatactt
     2401 atgaacgatg agttgattgc ctgtattcac tcatacccat ataaacaaaa tccaattaaa
     2461 caacgaatat ttaactattt tttactaaac caatccatta ttggggttaa aacattagca
     2521 tatttaagcg gaatgggtag aaataggaga ttttaatcat aaaaaactgt actatagtga
     2581 atttttggag atttatattc cagttatata ttaatgattg tgatacttgt actattgtaa
     2641 aaattcgtta ggtttaaata actggaaata tcggaaaatg ggcattatac atcacggaca
     2701 tcccttcttt tggaaaaaat gttacgattt ttataagtaa tattgtatcc ggtggaatca
     2761 acgatgaaga taacatatcc atcgttgtag attcagtagc attgaatgat gctcttgttg
     2821 caagaaatcc gaataaaatt gttaagtata aattgaatta ataggagata tgaatggaaa
     2881 ttagcagtaa agaatattta aaagcggttc ttcgtatgga aaagaatcta tatatagaat
     2941 ctaaaggaca acacacaaaa ttactaaaaa taatgaaatc acatccggac tacaacgctt
     3001 ggcggtatac tcgtcgaatg agaattacat cttattatta ttcaaagaga aagaaaaata
     3061 tcatttatgc gattatgtat attgtgtctt gcagaagaat gaatagtttg ggaagaaaat
     3121 gtggtataga gtgtggtgaa aatgtatttg atgaaggtgt aagattttac catacacaag
     3181 gaattgttat taatggtaac gccataatag gaaaaaattg ttacttatat gggaataact
     3241 gtattggaaa tgatggaatc gatcctaagt gtcctgtaat tggaaataat gtacgtgtat
     3301 gcgtgggtgc aaagattatt ggtaatgtaa aaatagctaa caatgtagtt gttgcggcag
     3361 gagctattgt tatcaaagat tgtctagaga atggtgcaat tcttgcaggt gtaccggcaa
     3421 aaataatagg gtatgttaat ttaacaaaag tatttttaga ataattttta gcgaaagaag
     3481 gtgaataacg atgcgtattt caataaaaag atggtcagga gcagattgga cattgttcac
     3541 gttggcattt tttgcaatct ccatagttct tgtatatact tttaacgccc caagagcaat
     3601 tttatatttg ggagatataa ttaatatctt tgtgttatta aatgcactta gctttagacg
     3661 cagaaaagga atatatgcta gcaaaaatga gatgctgata gttttgtttt tactagtttc
     3721 tatttgtagc tcagttatta atttagaaaa tataggattg attatctggg ggatacgaaa
     3781 taacactcgt atgttcatag tatatataaa ttgtgcaaca tatttaacaa ttaaagatta
     3841 ctataaagta ttcgctataa ttgaaaaatt attctggatt tcactgccac tttgtttaat
     3901 tgaaagattc atggtcacct atccaactgg aacaattgta ggagatatga taggtggagt
     3961 tttttggaat tattcaggtt gtaatatgcc attgaattta attttgtgtg tgacagttac
     4021 tgttgttaca atcggatatt tcaagggaga aaaaaatttc atccatttta ttttatgtgt
     4081 ttgtgcagca ctttttatgt ctgcattagc tgaattgaaa gtttttattc ctgaattatt
     4141 tataatattt gttgcaatag tattcacttt aaaagttgat tttaaagtag tgatcaagat
     4201 attatcatgc gtaatacttg tgtcgatagt attatccttt tttataacaa tatttattac
     4261 attgaacgat aatggacaaa gctatgctga taatttcaca attcaaggtc ttttagaata
     4321 tgcgtcaaga gattctggat atgatggtgt tggtgattta aatcgaataa ctggtatctc
     4381 tagagtagta gatgaaatat ttaataataa ttttagatta gtcctaatgg gagtaggcat
     4441 tggcaatgca gagtttacca atttttttgt cagtgagttt tataaatcgt ttggtaatct
     4501 tcattatcag tattttcatc atatttggtt atttatagaa gttggaatat taggagtttc
     4561 gatattccta ttttttataa taaagacatt tttcaatgca aaaagatctt ttatgaagga
     4621 taaagatgca agtcttgtta aagttattgc aattatgtcg gtattctttt ttgtgtataa
     4681 cacaacactt aggggtgaaa caacggggcc actgatttat atgattttag ccatgcctga
     4741 agttgtgaaa aggaaagagc attattatag tgaaaaaaat acaaaaggag agatattatg
     4801 attcctaaga agatacatta ctgttggttc gggggaaatc cacttccgaa aaaagataga
     4861 aagtgcatag aaagctggaa aaagtattgc cctgattatg agattattga atggaatgaa
     4921 tcaaattatg atttaaataa gaaccgttat atggccgatg catataaaga acgtaaatgg
     4981 ggttttgttc cagattatgc aagatttgat attatttata atcatggtgg attttattta
     5041 gatacagatg ttgagctaat taagtcgtta gatatacttc gtgaaaatca tgcctatatg
     5101 ggatttgaag gggaaatctg ggttaatggc ggtattggtt ttggtgcaga agaaggacat
     5161 tctgttatta aaggtttaag ggatatttac gattcattaa gtttttatca aaatgatggt
     5221 acattgaact tgaaaccgag tccgtattat attacagatt atcttgaatc tattggattg
     5281 gttcgcgata acagcgaaca aataataaat ggcctgcata tctatcctac ggattatttt
     5341 gctgccaagg attatgatac tggaaaaatt tataaaacta gtaatacaat aagcattcat
     5401 caatataatg catcttggaa aacacctaaa caaaaatttg tttatagatt aaaatcaaca
     5461 attggacaaa aaaatttaac aggttacttg cactgaaaaa atttttacac ttataaagga
     5521 gaaaagtact ttgaagaaaa tttgtttcat tagtggtgta atttctcgtt ctgggggaac
     5581 tgaaagagta gggagtatta ttgccaatag cttgtctgat ttaggttatg atgtcactat
     5641 tttaagcttt tggaatagtg gagagcctta ttttgggtta gataagacga ttaaggttga
     5701 ttatatgtta aatcctaaaa cggagggaaa attatataga acttatttat atccggtttg
     5761 gaaattgcac aagtatatat gcaaaaatga atttgatata gtaattgata ttgatacttt
     5821 gttgtctttg tatactacaa gggcaattaa aggaacaaag tgcaaattga tttcttggga
     5881 acattttaat tactggacaa tggaaatgct gaaagaaaag aagagattca aggctaaaaa
     5941 attgataaaa aaatatgcaa agaggcttgt ggttctcaca gaagaagatc gtcaaaaaca
     6001 tattgatgaa tatagtttat cagaagattt tgttataact atgccaaatc cgtgtatatc
     6061 taatatcgag tacgaatatc attatgataa aaagagattt cttgcagtag ggcgattagt
     6121 ggaagagaaa aattttgctg acatgataag tgcttggaaa attgtagaaa aaaaagtaga
     6181 tgattgggat cttatcattg taggaaaagg tgaattagaa aaagatcttc gccagcagat
     6241 aaaagattta aatctgcaaa gagtaactat agcagggcat tcagaccatg tggaggaata
     6301 ttataaatct gcatcttgtt atgtgcttag ttctgtttat gaaggatttc ctatggttat
     6361 attagaagca cagtcttatg gattaccagt tatttcttat gattgtaaaa ctggtccgag
     6421 agatttggta catcataatt ttaatgggat gttggttgaa gataagaata tagaccaatt
     6481 agctaagagc atgataatgt ttactaagaa tactgattta gcaatgagaa tgtcactgaa
     6541 cgcatataat aatgtacaaa aatttaattt gaaagaaatt acaaaacaat gggttgcttt
     6601 gattgaaaat gtatagtttt ttgtggttgt ccaaacatga aaattagcgt aattaatcaa
     6661 agagctagaa gaaacctatt acatttcaag attttagaaa ttatatagta ttaggtaaca
     6721 ataaaaaata gaaagtttgg agccatatga tatgataatt aatcgtaaac aaaatgctaa
     6781 aaggaatata attactggaa caatttttaa gttatatcag attatcattc ccttttttat
     6841 gcgtacactt atgatttatc agatgggaat ggagtatgct ggattgaaca acctgttcac
     6901 atctatattt caaatgttaa atttagcgga attgggaata ggtgctgcac taactgtaag
     6961 tatgtataaa cctatagcag aagacaatag aaaagagatt tgtgctttac tgaacgcata
     7021 taaaaagtac ttttcaatta ttggatggat agtgtttaca ttaggattgt tatgcattcc
     7081 ttttttgaga tttttagtta aaggttcgat tcctggaaat ttaaatatga cagttttgta
     7141 tttaatgtat ttgataaata ctgttttgtc gtattggtta ttttcatata gaaagagtct
     7201 aatatatgca catcaaagaa atgatgtaat taacgtgtct atgctaatta cttttacatt
     7261 ccaatttgta atgcaggcat atgttctgat tttcttaaaa aattactatt tgtatttatt
     7321 ggcttctatt ttgggacaaa tagttcttaa cttaatgtgt gctgttgttt ctcaaaagat
     7381 gtatcctgat tatactcctg ttggtgagtt agagaaagaa actattgacg atattataaa
     7441 aaaagtaaaa ggattagtta caaacaaggt aggcggtaca atattacgat cttcagattc
     7501 aattattatt tcatctttct taggtctttc agtacttgcg atttatcaga attattactt
     7561 tattatttct gccataatga gtatatttgc aataatttat gaatcatgta tagcaggtat
     7621 aggtaacagt cttattgtag aaagtcaaga aaaaaattat atagattttt gtgatattac
     7681 aacaattata ggttggtttc tggcaattag ttgtgcatgc tttattacat tatttcagcc
     7741 gttcatgatt atctgggtgg gaagtgataa tcttatgagt atttcatttg ttatatttat
     7801 gatagttcat tttttcttat atgaaattga tcagttaatt gggttataca aagatgctgc
     7861 tggaatatgg tacgcagaca ggtttagacc attaattaca gcaatgataa acattgtatt
     7921 aaatatcatc ctcgttaatt ggttcggatt atatggagtt ttggcggcaa ccattgtttc
     7981 agtattgttg gttgatttac cctggttatt atataatgtt ttccaaaata tatttaaatt
     8041 tgaatcggca ggtaaatatg tttggaagct cataagtttg tgtgtaatag cttttgttgc
     8101 ttcttattta agttattttg cttgttcgta tgttaatatc ggaaatttca aagtgataag
     8161 tttagtgata aatttagtta tatcggttgt catatcgatt ttagttattc ttctgtttaa
     8221 tatccgtaat aagaagttta agtatatttt gaaagttctt acgtcggtat tggtaaaaag
     8281 atgagaagag acatcagaat gatatataaa gtaactacaa tttttaggct gccgaatagg
     8341 cgaacgatgt atagtttttg ttccaacgaa gactcttgtt gaaattggag atgatatgaa
     8401 ggagttacaa ttttgactca tgtttatgat tgatcggttt taaaagtagt atggagaagc
     8461 tttgggatca tcggagagga tcaaaatagg taataatgtt tttataggaa tgaagtctag
     8521 aatactaaag ggtgtacata taggaaataa tgtagttata ggtgcaaata gtttagtaaa
     8581 caaggacgta tcagacgatt gtgttgttgc aggaaatcta gcaaaagtaa ttatgtccct
     8641 agatgaatat tataaaaaga taaacaatat gaagaatccg taaaacttgt gaaactatac
     8701 cgagaataat atgggaaaaa tattgatgaa aaagcattat atgagttttt agggctgttt
     8761 acagatggag atgacaattt gtcgaagagt tgggagcaat gcaaaattta gtaggggatt
     8821 ataaacagac tgacaaggta tttaaagaac atgcacaagt atttcataat attgaagaat
     8881 ttttgaatac tgtgagatag gagttgctac attgagaaaa gtatgtattt ttccaatgta
     8941 aaagattacg agtttgaaaa cgacaccata atattatttt ttaagaagat attatagagc
     9001 ttaggaacgg aagtttattt aaggatgttg aataaaatga aaaataagat taggtggatg
     9061 aaaaaggcag atgatttttt actataatta aaaatattgt tactatgtta tcaatcgtat
     9121 aaaaatactt actcgttatc aagacgatta tttcgaatat tttaggaaaa ataaattgaa
     9181 tattagagaa tgtgataata cactctaata tgatcatgtg taattggaag cgataagtgt
     9241 atacttgata ttggagaaat aattatgata atagtgattg cagaaattat tacagatata
     9301 ttatataaat tatatgaacc ctttgggttt gctgttttat tgacaatttt aggactggtc
     9361 tattttatgt atgcaacaga tggcattgct gcaggtgagg gctataaacg tatactaatg
     9421 gaactttatc ttcatttaaa gaaggataga aaatatagaa aagtttgtgc tttgatattc
     9481 tatactgcga tgattttatt tacaacatta atcaatcgaa caatttggtt aaatccttta
     9541 tctgatgtta tgggagactg gtggatttat aagtataata caacaactgg aacaaaagag
     9601 ttatctacag cgtgtttaga aaatattatt ctttttattc catatgttta tatggtaatg
     9661 tctattaaat atgaaaatat aaagaaaccc aaaatatggt cctcaatagt atatggagga
     9721 aaaattgcat tttatttttc agcaataatt gaatttttac aacttttttt gaggctagga
     9781 acgtttcaaa tttcggattt tgtatataat acgttgggcg gtatattggg tgtaatcctt
     9841 tataaactaa ttttgattac aaaaagagat ggtgaaagat gagactcgga ttgataataa
     9901 agaatatatt aaatttacct aaaacgatat acttcaatta taaatttctt catatttcag
     9961 atgcgtggag attacctatt ttggtgcatc gtaaagtacg gattacacgc aaatcgagag
    10021 ggaaaattga gatagtatta caatctggaa aaatagttta tttgggcttt ccagtattaa
    10081 atactgcggg aaatgaagaa acgattataa gtattgaagg aatactaaga atacaagggc
    10141 cagcgtttat cgcacaaggg gctcatattg acataaggaa aaatgcagtt ttagatattg
    10201 ggtcaaagtt taattcgaca ggaaaaatga gtataatttg ctttaaggaa attcaattca
    10261 aagatggctg tacagtatcg tgggatacat tatttatgga taccgattca catacgattt
    10321 ttgaattgaa tactcatagg attttgaata aaaataataa aatagttata ggtaaacatt
    10381 gttggattgg agcaaaaagc gtgatataga aaggtgttca tttagcagat aatattgttg
    10441 ttgcttgtaa ttccacggta gtaaaaagtc atgaaaataa taatgagatt ataggggggg
    10501 ctccagttcg aacgttgaaa agaaatacaa gttggtgttt gaacgagcca aagtgaaacc
    10561 aaataattat aaaactatgg aagtataata aagtttttga gaaatttgaa cgacaagacc
    10621 ctaaaaaaat ccatggacca ttattatgga tggacttccc aaaaggaata gccacttttg
    10681 agcacgtaaa tcccctaata agtggatcga catcgcttgt gcagcagatg atactatcaa
    10741 gaaaggtgtt tataatgtag aacttgaact aaatagattt gtaatcaccc attaggattg
    10801 taaagaatat gaataggata tatcttaaga tgaaaaaatt tgcaaattct aatgtatatt
    10861 tattaagggc actttgctgt gtttttttca gagataagat gaaagaaaga tggaagtctt
    10921 ggagtatacg aaataaagaa aaatattata tctgtttaat aagttgatat tgaacaaagg
    10981 actagtcgga gtcagcaaca cataagttgt gagtgatgct gactcgtttt tggcagaaaa
    11041 gaatcgaata gtaagtaggt gttttgatgt tgcttgacaa tggagagaga acccaagaag
    11101 attactagca aagcttatag atgttactgt agatttttaa ctccagatac agagtttgat
    11161 gaatattctg attatatcaa taacactata aaaaaggaaa tttccttttc acaggagaaa
    11221 ggtaaaagat attttcatag gagaatcaat gttaatttta aattatatga tttgtcaaat
    11281 taagctagaa taaaatgctt cttgtacata gctcaaaaat atttcgattg gtgttctgta
    11341 attcaatgat tttcttggaa tatgattgcg ttgattgctg acactggaaa taaatgtctg
    11401 attcacttct ctaaaatcta ttgatttcgg cagtccatta cgacgcagaa tcccgttaga
    11461 attttcgttt aatgggcgtt gagaaggtgt tccagggtcc gcaaaatata tatcaatatc
    11521 atgttggtta ctaatggctt tccagttaga aaattctttt ccacagtcaa acgtaataga
    11581 tttaaagaag tttttaggga agcgagaaaa ccattgatta agggcagttt caatatctaa
    11641 tgccttacgg ccgttgggtt taatcgtgat aatgacttta gataatcttt caactaaagt
    11701 aatgacggca cttttatgat gaatgccaac gatcgtatca ccttcaaggt gaccaaactc
    11761 agaattgaaa tcaggataat tatcaggtcg atcatggatt gagcgctgat actgttgttt
    11821 tccccgtttt tcctgatggc cattgggttt tcttttacct ttcatcggta gtgtgtcaat
    11881 atcaaatatt cctttagaaa ataaacgata aagtg
//
LOCUS       DACEMK010000086        11860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEMK010000086 DACEMK010000000
VERSION     DACEMK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.088"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(315..398)
                     /locus_tag="IW715_002093"
     tRNA            complement(315..398)
                     /locus_tag="IW715_002093"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="IW715_002094"
     CDS             926..1426
                     /locus_tag="IW715_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1022691.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="IW715_002095"
     CDS             complement(1616..1960)
                     /locus_tag="IW715_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1022692.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="IW715_002096"
     CDS             complement(2114..3034)
                     /locus_tag="IW715_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1022693.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="IW715_002097"
     CDS             3221..3712
                     /locus_tag="IW715_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1022694.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="IW715_002098"
     CDS             complement(3736..6111)
                     /locus_tag="IW715_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1022695.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="IW715_002099"
     CDS             6437..7063
                     /locus_tag="IW715_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1022696.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="IW715_002100"
     CDS             7086..7790
                     /locus_tag="IW715_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1022697.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="IW715_002101"
     CDS             7982..8815
                     /locus_tag="IW715_002101"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1022698.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9012..10940
                     /locus_tag="IW715_002102"
     CDS             9012..10940
                     /locus_tag="IW715_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1022699.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11078..11533
                     /locus_tag="IW715_002103"
     CDS             11078..11533
                     /locus_tag="IW715_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1022700.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
BASE COUNT         3592 a         2224 c         2143 g         3901 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaccgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctcttatt tgagaaatgt tctggttttt tattttcttt aataataatc
     8881 attataaata ataaaaacaa catcaagctt aatcaccaaa tgataaccat tatcgttctc
     8941 atttagctgt tattattatc aattacattt ttattatgct atccttgttt tgacaaggag
     9001 gtctaataac gatgactaat cagaaaaaag cttttgcagc aacaatcggc tgccttttat
     9061 ttatgatctt cggttttatc ttcgaccgat tctctcttac catcacccca cttttttata
     9121 tcagtgccat tatcatcggt ggatataaac aaacatcgga aggcttgaaa gaattgtgga
     9181 atgatcggac actgaatgtg gatttattga tggctttagc tgctattggt gcctgtttga
     9241 tcggtaatta ctttgaagga gcaatgttga cattcatctt ttgtttaagc ggtgcattag
     9301 aggaatacac aactaataaa agtcaaaaag aaattactgc tttgatgaat cttcaaccac
     9361 aaaaagccca attattaaaa gaaaatggac aaatgcaaga agtagatgtt tctcaactaa
     9421 aggtggataa cttggtattt gtcgctaaag gagcagctgt tccgatagat ggctttttag
     9481 aaagcacaca ggcaacgatc gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa
     9541 aatatttagg cgatgagatg tatgcaggaa cgctgaatca aggaaatccc atcacgattc
     9601 gagtaacaaa aacgagttcc gaaactgtct ttgctaagat tatccaatta gtagaagaag
     9661 cgcagaatac gccaactcaa acagcttcat ttatcgaacg aatcgaaaat aattatgtaa
     9721 aattgatttt gcttgctgtt cctctaatga tccttctccc ccactttttc ctaggttgga
     9781 gttgggatga aagcttttat cgaggaatgg tattattagt tgtggcttct ccttgtgcgc
     9841 ttgtcgcatc tgctacacca gcttctctag ctgctctttc aaattctgca aaaaatggga
     9901 ttttaatcaa aggcggtatc catttagaaa agctttctga attaaaagcg attgcctttg
     9961 ataaaacagg aacgctcact aaaggaaaac cagttgtaac tgacagcttt ttctttgatg
    10021 aaaaggattt ggcacaacaa cttcttgttg ctatggaaca aaaaacgacg catccacttg
    10081 ctcatgcgat tttgcaatat ttcgattgca caatacctga agaaatccaa tacttaccaa
    10141 tagaagaaat tactggtttt ggattacaaa ctacctattt aggtgcgcaa tggaaagttg
    10201 gcaagcacgc atatgatcct gagacgatga taatcagcaa agagattgcg gaaatgatcg
    10261 aacgtttgga aaatcaaggc aaaaccgtga tttatctttc taaagatcag cagttaattg
    10321 ctgttttagg tctgctagat attccaaaag caaataccca acaagtaatt tcctacttta
    10381 aatcccaaaa catccataca agtatgataa ccggcgacca ttctggcaca gcaaaggcca
    10441 ttgctgaaca agtagggatt gaacattact atgcaagctg tactccggaa gaaaaaacac
    10501 aactagtcaa aaaagagaat gaactttacc aagtaaatgc gatggttggt gatggagtca
    10561 atgatgcgcc tgctttagca gccgcttctt taggtgtcgc tatggggcaa gggacagatg
    10621 ccgctatgga tatcgcagac atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca
    10681 gtcatcggct atctttaaaa atgcggcgga tcatcaaaca aaatattatt ttttcgattc
    10741 ttgttattct cttgttgatt gcttctaact tcctgcaatt tttaaatcta cctcttggag
    10801 ttgtaggaca cgaaggaagc acaatattgg ttattctgaa tggcttgcgt ttactaagac
    10861 ctttacctga tctttctgat tcttcaaaaa aatgtaagtc ttgtccacta tacaaagcgc
    10921 ggctgtcttc tgtcaaataa gcaaagtgtt tttttatgat tttgtttatt ttatgaaaaa
    10981 aacagcttat gaataggtct tgaaggatga attcctactt ttgtaacttt ctaaaaacat
    11041 gttatgattg tttcgaagaa agtgaggaat atatttcatg gaaagttggc tttttttagg
    11101 aattatttta cttgtagcaa ttttagctaa aaatcaaagt ctgatgatag cggcagcttt
    11161 tgttttgatt atgaagctca taccagcctc ccgttccttt ttcccttggc ttcaaggcaa
    11221 aggaatcaat attggggtga cgatcatcac agcggctatt ctaatcccta tcgcaacagg
    11281 agaaatcggt ctacgtgatc tattagaagc ctttaaatcc cctattggct gggtagctgt
    11341 tttttgcggt gggctagtgg ctgtcttatc ttccaagggt gtaggattga ttgcttctga
    11401 tccacaaatc actgttgcac ttgttttcgg cacgatttta ggtgttgtct tcttaagagg
    11461 aatcgccgcc ggaccggtca ttgcttctgg tatcacttac tgtatcttac aagtatttgc
    11521 tttaggaaaa tagaacaaat tttgtctatt ttaggagatt cttaacaccc ccaatttact
    11581 gatccgtgat atactgacaa cgaggtgata caatgttcga aaaattaaag tatttcatag
    11641 gaacaatcgg ctggtttctt ctagcggtac ttttgattct gggcttagct tatctattat
    11701 ttcctgattt gatacaaaat gtgtttgata ctgctttttg attgacctat taaaacgata
    11761 taggggatgc tcctgcgatg caggggcttt ttttaatcaa tgttttgtaa taatttaggc
    11821 cgtgccaatg agacttccta tagaattcga tgctcttttt
//
LOCUS       DACEMK010000087        11744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEMK010000087 DACEMK010000000
VERSION     DACEMK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.089"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(123..647)
                     /locus_tag="IW715_002104"
     CDS             complement(123..647)
                     /locus_tag="IW715_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1022701.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IW715_002105"
     CDS             complement(640..1113)
                     /gene="tsaE"
                     /locus_tag="IW715_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1022702.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IW715_002106"
     CDS             complement(1236..2216)
                     /gene="pta"
                     /locus_tag="IW715_002106"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1022703.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2369..3049)
                     /locus_tag="IW715_002107"
     CDS             complement(2369..3049)
                     /locus_tag="IW715_002107"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1022704.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3175..4044
                     /locus_tag="IW715_002108"
     CDS             3175..4044
                     /locus_tag="IW715_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1022705.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4070..4585
                     /locus_tag="IW715_002109"
     CDS             4070..4585
                     /locus_tag="IW715_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022706.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4632..5195)
                     /locus_tag="IW715_002110"
     CDS             complement(4632..5195)
                     /locus_tag="IW715_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1022707.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5250..5429)
                     /locus_tag="IW715_002111"
     CDS             complement(5250..5429)
                     /locus_tag="IW715_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022708.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5506..6015)
                     /locus_tag="IW715_002112"
     CDS             complement(5506..6015)
                     /locus_tag="IW715_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022709.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6111..6491)
                     /locus_tag="IW715_002113"
     CDS             complement(6111..6491)
                     /locus_tag="IW715_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1022710.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6821..7570
                     /locus_tag="IW715_002114"
     CDS             6821..7570
                     /locus_tag="IW715_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1022711.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7754..9031)
                     /locus_tag="IW715_002115"
     CDS             complement(7754..9031)
                     /locus_tag="IW715_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022712.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9110..9754)
                     /gene="pcp"
                     /locus_tag="IW715_002116"
     CDS             complement(9110..9754)
                     /gene="pcp"
                     /locus_tag="IW715_002116"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1022713.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9953..10897)
                     /locus_tag="IW715_002117"
     CDS             complement(9953..10897)
                     /locus_tag="IW715_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1022714.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10899..11585)
                     /locus_tag="IW715_002118"
     CDS             complement(10899..11585)
                     /locus_tag="IW715_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1022715.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3355 a         2479 c         2057 g         3853 t
ORIGIN      
        1 agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc
       61 ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc
      121 ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag atctgttctt
      181 gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct
      241 ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc
      301 caactgatga tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga
      361 atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat
      421 gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg
      481 tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc
      541 aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca
      601 tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca
      661 tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac
      721 ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg
      781 cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata
      841 ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa
      901 cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg
      961 cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc
     1021 accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg
     1081 ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct
     1141 ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca
     1201 gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct
     1261 gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc
     1321 ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca
     1381 attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca
     1441 gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat
     1501 tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca
     1561 actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct
     1621 acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc
     1681 aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga
     1741 tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg
     1801 atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt
     1861 ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc
     1921 ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt
     1981 tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct
     2041 ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt
     2101 tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa
     2161 acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga
     2221 tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt
     2281 attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac
     2341 tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg
     2401 gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa
     2461 agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct
     2521 tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt
     2581 tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc
     2641 gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct
     2701 gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa
     2761 gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag
     2821 ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa
     2881 cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct
     2941 tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa
     3001 attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct
     3061 cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt caccctcagc
     3121 acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc
     3181 aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct
     3241 gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact
     3301 ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg
     3361 atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg
     3421 attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat
     3481 gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt
     3541 gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc
     3601 tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa
     3661 gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta
     3721 ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa
     3781 aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct
     3841 cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc
     3901 agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa
     3961 gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg
     4021 cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt
     4081 tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg
     4141 acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta
     4201 tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg
     4261 ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc
     4321 cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata tctataatca
     4381 tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt
     4441 ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg
     4501 tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt
     4561 ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca
     4621 ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc
     4681 tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca
     4741 tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct
     4801 aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa
     4861 taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga
     4921 tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa
     4981 ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg
     5041 taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct
     5101 gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt
     5161 tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct
     5221 aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta
     5281 atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact
     5341 ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt
     5401 ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc
     5461 ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca
     5521 tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt
     5581 gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt
     5641 catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc
     5701 ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac
     5761 ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt
     5821 ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc
     5881 tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc
     5941 ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa
     6001 catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga
     6061 acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc
     6121 agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta
     6181 tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca
     6241 atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt
     6301 tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat
     6361 ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta
     6421 acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt
     6481 gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag
     6541 ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa
     6601 ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat
     6661 gaaagtaaga aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt
     6721 tataattgaa agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata
     6781 cagaacacac tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt
     6841 tttagctcat cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtct
     6901 tggctgccag gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt
     6961 tatcggtacc acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa
     7021 cggtatttta ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa
     7081 ctatttaagt atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt
     7141 aagtaaagaa tggaatcata atatggcttc aaaaatccga aaattcactg gaaaaagctg
     7201 gtttatcgca atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg
     7261 ttatacagat gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc
     7321 tggtatcatc agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct
     7381 tgctcaaatg gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt
     7441 cgtgaacagc tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta
     7501 taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca
     7561 acacgcataa aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
     7621 aacacgtata aacggcggaa tcagaagcaa ctccttcgga aataagctga aattcacaaa
     7681 aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc
     7741 cagcctctat tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca
     7801 gtgattcttc taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat
     7861 aatgttttcc tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca
     7921 ctactagttg ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag
     7981 cttctcgttc tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt
     8041 cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt
     8101 ttggttggat gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg
     8161 ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat
     8221 gtactggcac ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt
     8281 tgatcaagaa ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt
     8341 tttttatctg tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat
     8401 tttccaatgc ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga
     8461 gccgctgagc taattttata tccggtaaat atggctgttt catccgacga ttttggtaca
     8521 agacaaaaca gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc
     8581 ataataattc gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca
     8641 ctgtcggaga gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc
     8701 cggtttttgg cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca
     8761 atttttgctg gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc
     8821 aatctgttgc agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt
     8881 gcttatcgac tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac
     8941 tgcttgtgta ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc
     9001 attcttcaaa gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa
     9061 agctggctac taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg
     9121 ttccggcatt tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca
     9181 aagaagaagc aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt
     9241 gatcgggtga gaatgacacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga
     9301 tcaaatgaag caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga
     9361 tgtacgctgg tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg
     9421 taaagtaagc ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat
     9481 tatcagggat tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc
     9541 gcccacctgc ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt
     9601 tgactacttg actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac
     9661 cgctgatttc atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac
     9721 caaatggatc aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt
     9781 aacatatggt tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat
     9841 agtttaaata acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact
     9901 acaatgttag aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa
     9961 atagaatcat ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt
    10021 tgatcactcc gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag
    10081 cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta
    10141 acgctggatt cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg
    10201 tgatggccgc aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa
    10261 tgatgatgcc gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca
    10321 ctgtcccaac tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca
    10381 tgcttaacgg ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc
    10441 gattatctcg agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc
    10501 cgactaaagc acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca
    10561 aagctggaat aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg
    10621 atggaagccg gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa
    10681 agatcaagcc accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta
    10741 agaccgtcca gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc
    10801 ttactccagt gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat
    10861 cgcctaataa aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa
    10921 tatttcttcg ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc
    10981 gtgatagcca ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga
    11041 tctttcagtg ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca
    11101 aagaaccagc agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta
    11161 ggatggactt ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga
    11221 cgaacaaatc cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag
    11281 atcccataaa tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa
    11341 gccgctgatt ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata
    11401 aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga
    11461 atgccaagac cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag
    11521 tctaatttaa atgcaaaccc gagaataaca atcaagacgc ctaaaaggct aagataattt
    11581 tccatatatt ttcctccata aaatgacaat atttttttaa ttataaagca aaacacataa
    11641 aaaatatagt tgttttctca cgaaatagac actattttta aatatttttc gttatttttt
    11701 tatcacaaaa attatcttac ttattgtaag gtaaagcttt tttt
//
LOCUS       DACEMK010000088        11340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEMK010000088 DACEMK010000000
VERSION     DACEMK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.091"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..936
                     /locus_tag="IW715_002119"
     CDS             <1..936
                     /locus_tag="IW715_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1022716.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IW715_002120"
     CDS             939..3122
                     /locus_tag="IW715_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1022717.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IW715_002121"
     CDS             3119..4120
                     /locus_tag="IW715_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1022718.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IW715_002122"
     CDS             4135..5049
                     /locus_tag="IW715_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1022719.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IW715_002123"
     CDS             5294..5410
                     /locus_tag="IW715_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1022720.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW715_002124"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW715_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1022721.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW715_002125"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW715_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1022722.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IW715_002126"
     CDS             9380..10642
                     /locus_tag="IW715_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1022723.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11340
                     /locus_tag="IW715_002127"
     CDS             10887..>11340
                     /locus_tag="IW715_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1022724.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         3735 a         1897 c         2376 g         3332 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
//
LOCUS       DACEMK010000089        11254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEMK010000089 DACEMK010000000
VERSION     DACEMK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.092"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(41..1420)
                     /locus_tag="IW715_002128"
     CDS             complement(41..1420)
                     /locus_tag="IW715_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAQ1022725.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1441..2988)
                     /locus_tag="IW715_002129"
     CDS             complement(1441..2988)
                     /locus_tag="IW715_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1022726.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3237..4016)
                     /locus_tag="IW715_002130"
     CDS             complement(3237..4016)
                     /locus_tag="IW715_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1022727.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4091..4549)
                     /locus_tag="IW715_002131"
     CDS             complement(4091..4549)
                     /locus_tag="IW715_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1022728.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4561..5820)
                     /locus_tag="IW715_002132"
     CDS             complement(4561..5820)
                     /locus_tag="IW715_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAQ1022729.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5936..6250)
                     /locus_tag="IW715_002133"
     CDS             complement(5936..6250)
                     /locus_tag="IW715_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1022730.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6396..7892)
                     /locus_tag="IW715_002134"
     CDS             complement(6396..7892)
                     /locus_tag="IW715_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1022731.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8070..8549)
                     /locus_tag="IW715_002135"
     CDS             complement(8070..8549)
                     /locus_tag="IW715_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAQ1022732.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8925..9170)
                     /locus_tag="IW715_002136"
     CDS             complement(8925..9170)
                     /locus_tag="IW715_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022733.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9275..10048
                     /locus_tag="IW715_002137"
     CDS             9275..10048
                     /locus_tag="IW715_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022734.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10277..10525)
                     /locus_tag="IW715_002138"
     CDS             complement(10277..10525)
                     /locus_tag="IW715_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1022735.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10624..10938)
                     /locus_tag="IW715_002139"
     CDS             complement(10624..10938)
                     /locus_tag="IW715_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022736.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3501 a         2317 c         1684 g         3752 t
ORIGIN      
        1 atcatttctt ccgttttaaa gaagaaatga ttttcataga tcaaccttta actaaagcta
       61 gattcttttc taaaaagaat ggtgtgtagt agcccacacc gctggacaac ggtaaaactt
      121 catccggcaa ctcgatgccc agttcgttac ggatgtactc ttggcgagaa atgattcgcg
      181 accaaacatt tggatattct ttggctagtt gatcacgaag cgactcatca gctagcgcaa
      241 tcccactctc acagcttgcg ccggcatagc cagcaactga tggaataata tcaatttgta
      301 gtaacattcc ggacttaaga gtaatttctg agtctttttt cattggcgaa gacatccact
      361 cttcatcagc cgtaagatgc cccggattta agtgccattt gtattccgat ttaggcaaga
      421 ctttgcctat tactgaatat agctctttac cttgcattcc tattttgata ttttccaacc
      481 atgcgaccac ggcgtcgtag tatggagctg caactttttc aatgtaatcc ttttgccctt
      541 ctggtaggtc atctgtcgtt gcaactgcat agccacttcg acttgctaat ccgcctttaa
      601 agcccgtagt aattgacatt ttatcgccta gtttaacttg tttatcagtt ggatatatat
      661 aggcgttctc aaaccgttcc ccagttgcag caattggaat gacattattt ggttggccgt
      721 acttaactaa gtggttacca atttccattt ctgtttttcc tacctctact tcatcaatag
      781 cgttcatcac acaatctgaa gccaaggatg agccaaattc ataatatgcc acttcattag
      841 cagtatttaa tgagcgtact ccgtaatcac tgtgaatgaa caggtcggtg cgattgctaa
      901 cggcatcttc ccctactaat tctacaatcg aacttacaat gaatgccggc aaatcttgga
      961 tttggtagtt atcctcttct gaagtaaaca tcttccaacc aacaagccca actttcttac
     1021 ctttactgat tttagcattg gtcaatactt cagttagtgg tttctcgtta tccatcggtt
     1081 gatccggtaa tgagaagagt ggcgtgtgga ttaattctgc ctcaattctt gaatagtcca
     1141 ccattttagt attttcattt cctagaacta agaaagcttc acccgtctga tgtaatacca
     1201 acaagctttc ttcaaagcga gtaacaaaac ctgttaaata agagaagttc ccgccatgtt
     1261 ccaaatcaca atagattacg agggtatcta tctctgactt ttccatcgct gcaagtacct
     1321 tttctttacg gaacttcatt gtttcatcag tcaattgata tattttagct tcattatctt
     1381 ttttaggtgg gttaactttt tttagagtta ccttagtcat attgtcacta ctttctgttt
     1441 ttatttacgc ttcatttcat ttttcagaga tcgagttgcg ttttccactt gtcctccata
     1501 taccacttgt aaattatttt cgttaatttt aacgattccc attgagccgg tctttttcaa
     1561 gatatgttca tcaattgttg ctgtatcttt aagaactagt ctcagccgag agatacagtt
     1621 atcgatttct tgaatattac cagcaccacc aagtccttgc aagattagtt tgccaagttc
     1681 gttttctgtc atttcttcgt cctgatcttc caacacatca tcgttggatc gaccaggagt
     1741 tttcacatca aatcgctcaa tgacatactt aaatacaaag taatatgcaa ctgcataaac
     1801 aaggcccaat gctacaacta agtaccaacg agtataagta ccttgtaaaa ttccgaagat
     1861 agccaaatca ataaatccac caccggcatt cccaactgcg actccgaata gattcattaa
     1921 cataaaggat aaaccagcca tgaatgcatg gaagacccat agaatcggtg agataaagat
     1981 aaaggcgaat tcaattggct cagtaatccc ggttacaaaa caagtagccg ctgctgcaat
     2041 caacatccct ttaatctttg gacgttgcgc caaatatgaa gttttgtaca ttgcatagca
     2101 tgctgccggc aatccgaaga ccataaatgg atgagtacct tgtgtcaaga agcgagtagc
     2161 acttctcaaa atatccatgt cttttactgg caatgctaat tcagtattga agatggttaa
     2221 tgctccagaa actgtttcac cattgatagt tgccgtaccg ccgattggtg taaatcgaat
     2281 caactggttt agtacatggt gaagacctgt tggaatcagc aggcgctctg agaagccata
     2341 taaaaaagtt ccaattacgc ctgacttacc aatcaaaccg cctaaagcat tgatagctgt
     2401 attgacaaaa ggccagatga aaaatgaagc gattgagatg attggaatag ttaggatgat
     2461 cataattgga acaaaacgat tgccactaaa gaaactgaaa gctgctggta attgttttgt
     2521 gtgatagcga ttgtggatca tggctgtcca taatcctgca atgataccgc cgaaaacacc
     2581 catacgatat gtgaatgtgc caagtaaagt ttcatacata ttggaagttt ctactgcttt
     2641 gatttgactc attccgtcag acatcaatgt ttcaacagaa gtgctttccg ctgtgatgcc
     2701 tctaagctga agcagataat tgataactac atggaacaga ataaatgcaa taaccactga
     2761 aaatgctgca atttccttat cttctctaga catcccgatt gctactgaaa tagcaaatag
     2821 aataggaagt agtccaaaca tcgtaccaac aatagcctgt actccaccaa caaatccttg
     2881 gatcacggtt gcattagcga agctttctcc caccaaagta ggattttgta aaattgaaac
     2941 gattcctagt agtagtcccg atgcgacaat aatagacaac ggacccatta atgatttccc
     3001 taacttctgg atataagttc tcaatataaa caccttcttc tttttagttg atataaacgt
     3061 ttacatccct tgtacaacga aagtatagca ccctgaaaaa gcgctaacaa tacgataggt
     3121 ctgataaaaa tcaacattca gcacttggtt gttcattcaa aacgtgaatc ttcaatcaca
     3181 gaaaaatacg cgctatcaag cacgtatttt aggtgtaaat atatgcatca actttattaa
     3241 tcaacgctac tttgtttgta atagttaata tacccttcat acagtaagct aagagcaaga
     3301 ttcaaggtgc agaatgatga gttgttcaac tcattcacat cattaatgtt ggtcacttga
     3361 tagtataggc tatagtctga cagactggct aagttatttt gaccaaatcg tgtaactgag
     3421 accaactgtg tccctttcat atggattctt cttaaagtat catcaatccg atcaacacgt
     3481 ccggacaatg atatgacaaa tagtaaatca ttcgaggtca aagcttttga aatcaggtct
     3541 aattcattct tttccggtac aacgataatg tagattccca agttccacat gcagcgtgca
     3601 aactcattga gcatcattct ttgtccatat cctgttgcgt aggcaaatat cttatctgcc
     3661 tttctaatcg tttcatatat atcctcaaca cttttatcat cttcaaacaa tttggttgtc
     3721 gcttgaacat ctccgagtaa tacttccggt aaaaacaaac tacttttatc gactctggtt
     3781 ttttgcatgc gtgcaaattc ctctttaagg taatatcgaa attcgccata tcctttgaat
     3841 ccgagtttct tagttagtct aataatcgtt gagtcagaca catttgcttg ttttgctaat
     3901 tccgatacag tttgactgat gctgatttcc atattttgag tgataaaact taatacataa
     3961 tcatcattct cattcaattt actgtgatgt tcatttacta attgctccaa tgtcataatc
     4021 agacctccaa aaataataaa aaagattgct ctctcatttt aatacgaaag cacaatcttt
     4081 ttaagtacta ttaaattttg aaaagaagtt ctccttcaga aacctttttt tcagagactt
     4141 ctagtttaat acccttatct tcgggaaata gaaccataac aacatctgac agtttttccg
     4201 ccttgatttg gtcacaatca aaaatcacca atgactcgcc ggatgatact cgttgacctt
     4261 cttcaacgag cacttgaaac ccgactccct ttaactgaac agtatcaatt ccaatatgaa
     4321 tcaataaatc tctaccttga ttactagtca atgtgagtgc atgctttgtt ggaaatatcg
     4381 atttaatgac accgtcaatc ggtgctacca ccttagcgtt tgttggttca acagcgaatc
     4441 cttcgcccat catcccttgc gaaaatacag gatcgcttac ttggctaagg ggaatcagat
     4501 ttcctgatac tggagcaact aatttttctt ttcgactaaa aaatcccatt gacttactcc
     4561 ttaatctttt gaaatgtcca tcacaaggtt taaaagaaca tccgcccctt ttttgacatc
     4621 ctcaaaatca gtccactcat caggatgatg tgtaatgcca cctttggatg gaatataaat
     4681 catatttgtt gggacaaagt cggtcatcat catcgcatca tgtccagtac cattgttaat
     4741 gacttgatag ctatagccca actgatcgca aatatgtttc atcttctcaa cattaccttg
     4801 atccataaca cttggatttg taggattact gtagttaatt cgagccattt cctccagctc
     4861 agttctaact ccagtttccc cttgaatcac tgataactca ttcaacaact tttctttaat
     4921 ctcttgagga ttagttgtct ttgtatcaaa ggttctgatt tcgattaaag cttcgacaaa
     4981 gtcagggaca aattgatttg agttcggttt gattgtaagt ttaccgacgg ttccggcaat
     5041 cccttcacca agattcttca tccactgttc aacaaaaaga acaaatttag aagcagcaac
     5101 taaagcatct ttccgtttat tcattggagc agtcgagtcc tcagtttctc cataaaaacg
     5161 tgctttaaaa cggccgatgc caggaataaa ttcaatcaat ccgatatctt tgccttcatc
     5221 ttccaggact ggtccttgtt cgatatgaag ttcaataaaa tttttgattg tttttggatc
     5281 tcgcttagcg gcctctaaat ctggttccac tccataagca cgaagagctt ccaattttgt
     5341 ttgtccatgg cgatttttta ctgtttccaa ctcactcatg gttaatgttc ccatcatagc
     5401 tcgtgaattt gagacacccg tacttggccc aaatgtctcg ccctcctctg cattcatcag
     5461 aattaggtca attgggtaat cattttcaaa attattttct tccagaatcc gcattgtttc
     5521 cagtgcagta acaacgccag ccactccgtc aaatctgcct ccattaacaa ctgaatcata
     5581 atgagagcca atcatcactg atggtgcatc agccttttta ccggctctac gaccaaataa
     5641 tgtagagaag ccgtcttccc aaatctctaa acccagttgt tccatttgat tgatcaaata
     5701 atttcttgct tcacgatctt ctttagagta agcagagcgg gtcactcctt cacctggagt
     5761 tgcattgtac tcggctaaat tttcgatatc cttttttata cgctcaaaat ttgtcttcat
     5821 aattaaatcc tccatttcct acaagtgaat gttagcaccc atagaaagcg gctacaatat
     5881 aatcaatgaa gattgaatcg atattcgaaa actgaatatt gcttcggtta atgacttaga
     5941 aatcattatc gtagattcca ccataagaac tttttctcgt ctcataacac aactcgagag
     6001 tggattggtt taaaaaactc tcgataaatg cgtccacttt attgacacag atccatggat
     6061 cgatcgcacc ttgaaattta aacccagaaa tagtcccatc atcttctata ccgattacca
     6121 gcttacctcc atcggcattt gcaaacgcta ctatgtgttt tataatatct ctaggcttaa
     6181 tccgtgcact ttttctatca aaatactgat cttctttttc acttataaac ttttgaatct
     6241 cataatccat ttaaaatttc ccttcttaaa tttttttatt atagagccgt atattcaatt
     6301 tccacaaaaa aataatctaa aacaaaacag acaccttcat tttgatatgt tctcccacat
     6361 atgaatcggt cgctatacag cattacatat agtaattatt catactgcga aataaatagc
     6421 tccaaatctt tagctggcgt agttaagctt aaattcgaaa tgaaagtttc attgactatt
     6481 ccactttcat caaatccctt cttcaaccaa tcagagttcc acaacgtaaa tcctttagga
     6541 ttagaaactt tcttacataa ttttgcacta taggatttct ctcttgaatg aattaaagaa
     6601 agtctttgta accaaatttc aactatatcc gtattaggag tattaacaaa ttttcttaag
     6661 attttatcca agtataagtt gatttcttct acagcaataa actcaaaaag cttacctaaa
     6721 atcacaatac aagcctccaa agttctagga ttcttttgca ttatattaac aagaatacta
     6781 atcaattggt agatatcatc tggtcttttg tttaaagtag aaactctatg tgtatagact
     6841 tcacttagcg ctttacttaa actcccgcaa tttggatatt tgtctcctaa aagctttacc
     6901 tgtagcaaat gcttttgaag gctaagctta aacttccact tattattact tttctctgta
     6961 aaacttgcat gtttaagatc ccagtaaatt ttatcaggct taattgcatc taaaattata
     7021 ttatccgata agaatgtttt atttgaattt agcttcaaat ttagatccga taatacttca
     7081 gataacaatc tgataatcac ttcagcttga tcttttcgaa ccgaaaaaat tctgtaatca
     7141 tctctgtaac gaagtatttt gaactcttta atctctgttt tttctatctt gttcagtaac
     7201 attttatcag catacccaag tactatttca gctacaaaat ccatcaatac atttccttga
     7261 ggtataccgt tcgtttgtcc attttgtaaa tatcgtattt tagaatctat taagtttcca
     7321 acgccttgag tatcttttct atgtgtctta gcccattctt ttgaatgtac tgcccacggg
     7381 atagtatgtg tgtacattga gctataacaa tcggtaatat ctgaatgaat gcagtagtca
     7441 tactccaaag cataactaat ctgtgcttgt tcaaggttct cccaccaatt taaaatagtt
     7501 tccgcagtat cgctctttga agaaatagat tccacaggaa gtgatataca ttcaattcgc
     7561 ttgtcggctt ggaattcttc aaacttattt ttgatgtgat tccaattatt tttctgtgta
     7621 atcccgttta ctaagtctac atatactaat ggatgtaata tttgaagggg tctccaagca
     7681 taattaccat ctttattagc taatattcga tgattaacat caactgcctc agagagcgat
     7741 ctcttatgtc taactagttc agtaaatggt ttgtttttta ataaatccac tgctttctca
     7801 atgcctactg ataaatcaat atagttgggc aacgatgtac taaaaaaact tgatgttttt
     7861 aaaaagaatt gctttgcttc ttgatgattc atctctaaaa tactttttct agtcctagtc
     7921 aaattcaaaa tctcctagat caattaagtt gtaatgtaag aggataccct aaggttacac
     7981 accctattta ctattatacc agtattcatt cgtgatacat aagggtgtaa cattaagaac
     8041 tctatctaaa agactacaag tagtcacctt taattgtgag taattttctt tatccaatcc
     8101 ttgtgcaacc ttgtcgtcac cccttgtata tccccgtacc ccggatgttt cggaatatca
     8161 aaaatgtgct caaattcctg attttcatca gtctcccact catgattcaa cggttcatgc
     8221 cactctataa accttgacca taaaatgttc tttctataaa actcaatagg aatttcaaaa
     8281 cagtaaaaat tgtatttgct aaaatctgta aaattatctt tacgagcata ctttaaatgg
     8341 caggtatctc gccacaacca aattaaatct cgatccaatg gtaactgaat tcgtttcgta
     8401 tactcttctt caagccaatg aagtgcacca actgcattcg gattatcgaa aaagacagat
     8461 aattcagtat agcgtttctt tggataaata aatccttctg aatcaacaga ttgcaaaaaa
     8521 tctactggtt gaaccgtgta cactttcatt tattcgccac ctttttttga ctacttatag
     8581 tcatttacca atttgaaatc tgcatacttt ttcattcaat tgagcccagc attaattgta
     8641 cacgagtttt cttcactaac ggatagtatc aggatatact aatactatcc tgaatagcga
     8701 aatgaggtag ccaaaatgag aaaagtcgat gaatatatca aaaagaaatc ccaaaaaagt
     8761 aaagtatggc aaagaacatt tgagttagag tataagaaac tctcagatag cctcaaaaag
     8821 gacacttata atccaaatga acaaagtaaa gcagataacc aataaagatt accctcccat
     8881 atttaacatt gcttcacgaa tcagtaatca atgactacaa gtagtcattg aagcaaatct
     8941 tcataagata cctgtagaat atccctaata gctctcaatt gatccagctt aatatgttgc
     9001 cggccacctt caatcttaac caatgtttct cttgtcatag cgattttact gagctggagt
     9061 tctcgcacca gttctgtctg tcccattccc ttttctaatc ggattcgtct gatattgcta
     9121 ccgattacat tttctttatc tattaacttt tcttccttca aaaatagcac gccttttgga
     9181 ctaaaattag tcctttttct tattaatctt aaagatttgg tatgctacaa tgggactagt
     9241 attagtccat attcaatttt aaggaggaat ttttttgaag aaatttgcaa tcattctaac
     9301 ctgtgcttta ctcacgggag ctataagtgg gtgcgccact tctgacacaa attctacaag
     9361 tactgaaaca agtacaacaa cctcagaaaa gaattatcct atacattttt ttccagttcc
     9421 agatggattt gaagcttatt atcatgataa aaaactagat gttccattgt cagaaagtaa
     9481 ctctattgaa aaagttttgc caattgaaat tgaaaaaaat caagagaata taaaactcaa
     9541 ggccgagctt cccatactga acagcacaga aattgattgg cacgagaata ttaaagtaac
     9601 tattgaaaaa ggagcgttag cacaacttta tactgaaaaa gttgaacaat tttttgagtc
     9661 tggtgccaat aaagactttt caaaaataag cgattattcg gataactttt ttaatgagac
     9721 ccaagatgaa atcacgaact caacttttat ggctgaagat atgtacaata tgacgttaaa
     9781 aaaaataaaa gcttacgacc atccattaaa tctgacacta aataatggat tactctcttt
     9841 tgtattagca ggttacgttg actatgaata ttcaccagat tatttggaga cacctcttca
     9901 acacgagaac aatgatatta attttagatt tgtctacgag tcaaaaagta aaacatggtt
     9961 agttgatcaa ttaagcacta taaacacaag cttccatgaa ttacctaaac ctgaaaatga
    10021 agctgatatg gtcattaaaa tcttctaaaa aagaagcgtt gaaggagaaa tattcctttc
    10081 aacgctcttt tttatatgct tagaaacata aataaattgt atactttctt tttagcccct
    10141 attacttttc acgaaaaata tcttttttcg acactagcgt ttctctactt ataatattgt
    10201 acacagtttt tgtattattg aaaaattact cattatttag cattaatcca catcgtttta
    10261 tcaatcgacc ttcatactaa actactttaa agtcatcttc cttctcattt ttcattaaat
    10321 cagatacata agcccatgta cccgttcgtt gtaaataaag ttcttcttgt agcagctccc
    10381 actttctctt agaaatcata atgacgccat cgttcaatcc atttttagaa tccctagtaa
    10441 tttctatttc agaatttgaa tgaactactt cttgcaacac tttacctata ttttttttga
    10501 agtttgttgg agtaatagta tccaaaatga acacctctct tctctatacc tctattatga
    10561 agcgcccatc aaataaagca agcgttgagg aataattctt tcaacgcttg ctttattaag
    10621 ctttcatttt gtagtttgac taataccaat aatctgatct ctaaacaatc gaaaaacttc
    10681 ttgtggttga taataatctg tatctatttc aaatgtttct gagaacaatt cattgaattt
    10741 ttctactgaa atgatgattt taccattctc ctctcgactg ttttctatta tttctgcttc
    10801 taattcaaat aaatctagtg acgaactaaa atcataaatc ctgtcatatt gaacaaaaaa
    10861 atcaagaaaa agttgctcaa actcttcctt atttatcact agtgtttcaa gcggaacaac
    10921 tttattttca tcagccattc ttccaatttc ctcactttca ttttcaaaaa atcgtaaaat
    10981 tgatatagat tctttgtctc taatcactcg tgatattatc ttgacagtcc tacatctaat
    11041 tcaattaaag tataagccac tttcaaataa aagattacaa agtaaatggc aagccaacaa
    11101 aataaccatc tgcttcacgt attacgattt gataattctg gatttcgctc ggaagttcat
    11161 aataataatt aacagtttct gctttgccac tttcaatata ttctggatta ttcaatatca
    11221 tagcatactc ataaagttct tcaccatttt tatt
//
LOCUS       DACEMK010000090        10929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEMK010000090 DACEMK010000000
VERSION     DACEMK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.093"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            1..407
                     /locus_tag="IW715_002140"
                     /pseudo
     CDS             1..407
                     /locus_tag="IW715_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(309..1025)
                     /locus_tag="IW715_002141"
     CDS             complement(309..1025)
                     /locus_tag="IW715_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1022737.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(1149..1334)
                     /locus_tag="IW715_002142"
     CDS             complement(1149..1334)
                     /locus_tag="IW715_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022738.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            1561..2834
                     /locus_tag="IW715_002143"
                     /pseudo
     CDS             1561..2834
                     /locus_tag="IW715_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2941..3972)
                     /locus_tag="IW715_002144"
     CDS             complement(2941..3972)
                     /locus_tag="IW715_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022739.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(3979..4809)
                     /locus_tag="IW715_002145"
     CDS             complement(3979..4809)
                     /locus_tag="IW715_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1022740.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(4806..5612)
                     /locus_tag="IW715_002146"
     CDS             complement(4806..5612)
                     /locus_tag="IW715_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1022741.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(5618..6442)
                     /locus_tag="IW715_002147"
     CDS             complement(5618..6442)
                     /locus_tag="IW715_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1022742.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6432..7532)
                     /locus_tag="IW715_002148"
     CDS             complement(6432..7532)
                     /locus_tag="IW715_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1022743.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7710..8495
                     /locus_tag="IW715_002149"
     CDS             7710..8495
                     /locus_tag="IW715_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1022744.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8528..8911
                     /locus_tag="IW715_002150"
     CDS             8528..8911
                     /locus_tag="IW715_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1022745.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            8987..9118
                     /locus_tag="IW715_002151"
     CDS             8987..9118
                     /locus_tag="IW715_002151"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1022746.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9087..9323
                     /locus_tag="IW715_002152"
     CDS             9087..9323
                     /locus_tag="IW715_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1022747.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(9401..10372)
                     /locus_tag="IW715_002153"
     CDS             complement(9401..10372)
                     /locus_tag="IW715_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1022748.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3249 a         2541 c         2088 g         3051 t
ORIGIN      
        1 agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat tatccgcaac
       61 ggttcctata cgacacgtac tcagatgttg aaagtcaaag aaaagttgac tattttagaa
      121 ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catagaactg
      241 tacgaaaatc aatcacgtaa tctgaaaatc agaagaagta ccgtcggctc aagcgatatt
      301 ggaaactctt actcaaagat tccacgacat tggagccgct caaacggcat tatcatcgac
      361 tgtttaaacg accaattagt caaacagaaa tcgtcgatga accgttgtgc acaatggtgt
      421 cctgaacgta cagcgacacc ttccatatcc agagcagtcg ccacatcacg gggatggatg
      481 ccatccaaat taaaagcaac cacgccagta tgatgttcag gatcttgcgg tccgtaaatg
      541 gtgataccgc cgatcgcttg taatttaggt aaaacgtagg caaccagttc ttgctcataa
      601 cgatgaatat tctccatacc aatctcattc aagtaatcga ttgctcctgc aatgttcgga
      661 gtaccagctt caaatttcca tggcagctct ttccatgaac tatcgtaaag attgacaaaa
      721 tctatcattt caccgccgaa ttcgactggc tccatttgtt ctaacaattg acgtttgcca
      781 tataatacac cgattcctgt cggcccgcac attttatgcc cgctaaaggc gaaaaaatct
      841 gcatccaacg cttgtacatc aactgtcata tgcggtgctg cttgggcacc gactggacta
      901 tcctcaacta caacagcctg ttcaaccctc agccgtttca agcacaacaa ataaccttct
      961 ggatctggtt tgccgacctg aagatcatcg atcgtcacaa aggaatcaat tttttcctta
     1021 atcatcggat gtgcttcaaa aaaatagtct agctcccgca gttcagaatt gagactactc
     1081 cgattttcca gtgctgctga tccgctgctc aaaatgttca gcatccactg gtctcccaac
     1141 cgcttgactt atcccttcat aaaaggacag ttctgctgcc gtcccaacac cttgttgata
     1201 gtctgcaaaa ggaattttat agccgaactg ctccagtaaa taggcttcag taaatagtga
     1261 tgaatactct cgtaaaacat ccattcactg tcaatcatcg tcccatcaaa atcaaaaata
     1321 agtgctttgg tcatcttctt tcccccttac ccaaaacaag caaaagctgt gtcctgctct
     1381 ctctacagta tcagcataaa aactgccgtc tgaaaagtca aaggtgaaag aaatgtggcg
     1441 ctataatttc taggactgat agtaaatcat tctatcagtc cttttgagca taaaaaaaca
     1501 aaacatacag aagctccgtt agaattgaat caccacaaca caatcaaagg agcgttctgt
     1561 atgcctgatc ttcatcttat caaactatta ctaggaatca aagatggaaa cattcaaatc
     1621 actcatgtcg agaagaaaaa aatacattca atcaacagta tcgttgtttc tgccacgctc
     1681 attaaaacga ttcagccctg tcctcagtgt aaacaggtga atcaacatgg gctgatcgtt
     1741 aagaacggaa agaaaaaatc gctggtcctt ctcaataagt gtgcgaatca acggacttat
     1801 cttgctttgg caaagcaacg gtacctctgt cgtggctgtc acacctattt tacggcgaat
     1861 acctacattg ttgaaaaaaa ctgttttatc gccaaacaag tacattataa aatattggag
     1921 gaattgactg aaaaacaagc aatgacgacg atttctaaac attgcggcgt ttcttggtca
     1981 accgtttcgc gaacactcgc ttctctgctc cccatgacaa acgtgaaaat aaattggttg
     2041 cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa atcaagtcgg tccctattct
     2101 ttttcttgta tggatggcga tactgggaaa ttactggata ttcttccttc acgtaagaag
     2161 aaagatctca tttcttattt tatgcaattt gaacgacgtg cacgtttaaa cgtaaaaata
     2221 ctcgtaacag atatgaacgc ctcttatgct ttgctgataa aagagtgctt tcctcatgcc
     2281 aaactcgttg tggatcgctt tcacatcgtc aagcacctca ttcgcagttt taaggatatt
     2341 tgactatgag tcatgaaaag cttcgaccga aacgatccaa tacaagccaa acactatcga
     2401 caagtgaagg ctttatctcg acttctgatc aaacgacagg acacgcttgt ttatgataaa
     2461 tggacaaaat ggcgtaattt tggttgggcc tatttaaccg aaagtgaagt cgtagaacgt
     2521 ctactttcta cttctgatga actacgaata gcttatgctt actatcaaga aattcttcaa
     2581 gctttttatg acaaagaagc ggacatcttc tttcagcttg taaaaacaat gcccaagtcg
     2641 gttccaagag aactgcatca tataaaaaaa gcgtttataa actatgaatc cggcattcgt
     2701 ttagccttag agttgcctta ctcaaatggc aaaatagaaa atctgcatac ccacatcaaa
     2761 gcattgaaac gaatagctta cgggttagga tgagaaccag aattttctta atcaatcatc
     2821 tgatcactta ttaaacataa aaaaacatct aaagcagaaa atcaattcta ccttagatgt
     2881 tcttatatca ctctatcagt cctatttgac aaagagccag aaatgtttgc cgactataca
     2941 ctagctttgt tgtgcaaaat gctgagtatc aatttccaca gggattgcta gaatatcttt
     3001 cagctgaatg tataaggcat cgctaatttt ctcttcccga tcgttcaacc gatccactaa
     3061 aatttctttg ccctccagcg tttgaaaaac ataccgttga atgttgccta aaataaatga
     3121 ttccttccat agaaccggaa tcttaacccc ctgaggaacc ggttcaaatt gaatcagctc
     3181 cggtcttaaa taataaatgc agcgattgcc cttagcggaa aactcgtggg tatactgatt
     3241 cagctcatac cattcgaaac gattatagtt gccgataaat tccgcaacaa atcggctctg
     3301 aggattctga tacaattcct tcgaggtgga aacctgagcc acccgcccag cttccatcac
     3361 aaaaatccga tcactgattc gcatcgcctc ttcttggtca tgagtcacaa aaatcatcgt
     3421 catccctagc gattgctgga tagcgcgcaa atcccgctgc agctgttttc taattcgggc
     3481 atccaaagca cttaatggct catccagcag caggactttc ggttcagtga ccaatgagcg
     3541 agccagtgcc acccgttgtc gttgaccgcc agaaagaaac tggacatttt tatctgcctg
     3601 ctccgtcata tccaccaatg atagcatctc aaaaaccttt tcctgaatca cctcaggact
     3661 cagcttcttt accttcaaac caaaggctac attttcaaaa acagtcatag ttggaaacaa
     3721 tgcatactgt tgaaaaacaa agccgatatt gcgatccttc gtaggcttat cggcaatatt
     3781 ctcattgtcc agcacaatct caccgctctt tggttgttcc aatcccgtga tacagcgcag
     3841 caacgtagac tttccgcacc cgctgggacc tagtaaagta accagctcac ccttttcaat
     3901 ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa aacagattga gatcatgtat
     3961 ttctaaaaac attttccgtc acaactcctt taagggcttc tttttcgact gcttctcttc
     4021 cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa gtcagcataa aggtagttaa
     4081 agcaattccc accataaaca gcaaatacaa aaccatcaca gcgctagcca aatggccgtt
     4141 ttcgttcatc cgccgcagca tatagatgcg taccgtctga aatcgaccgc caatcaacag
     4201 gttcgtcagc atgtactccc caaaaaccga agaaaaaatc atcagagaaa ctagggtcac
     4261 accgatacgg atgtttggaa acagcacctg gataataatc gtagacatcg gggcgcccaa
     4321 ggtttctgct gcatccacta attccttcag attaattaag cgcatctggt tgttaagact
     4381 aagatagaca atcggcaacg ccgtaataaa caatgcacct accaacacta caaacattgg
     4441 gataccagtt tcagcatacg ttcgaagcaa agccgttacc aaaatcactc ctggtaaagc
     4501 atacggcagc aggagcagct tttccagcca acgattcaaa cgagggaaat acaaatgaat
     4561 ccaaataatg gtgggaatca tcaacaacag aatcgtcacc aagaccacac ctgctaataa
     4621 aagtgagcga cccaccgcag caataaattc cctgtccgtc agcagctgtt ggaaccattg
     4681 caaggtaaaa tcactaggta aaagcgattt tgaccattta gtggagctgg catacaaaat
     4741 cgttccaata atcggcagca agtaatacag gacaaagcag aacaaaatcg gttttattaa
     4801 ctttttcacg tctgcaccac cttgcctttc ttttgaataa tcaacattgc cgttaccagc
     4861 aggacaccta aagcgactgc caacgcactg cctaaattag gcttggcgaa cacatctccc
     4921 gacactaaag ccgcaattcg aatcgttaaa agatttacat tactgctggt cagggcatac
     4981 gctgtttcat aggtccccat gccattagca aataagatga caaaggtgct ggcaatcgat
     5041 ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg ctgatgaagc gcccagcaat
     5101 tgtgcggact ccagccattc ctttttcact tccttcatgc tgggatacaa aaacaagacc
     5161 cctaacggaa tttggaaata ggtatacgtg atggtcaatc cccaccaaga atacaaattg
     5221 aagtccgcta gaaatggcaa aatcattttc agcacaccta cattccccaa caaaataata
     5281 aaagagaatg ccagcggaat cccagcaaaa ttagctgcca aattggaaac aaaggtgatt
     5341 ttctcctgca atttagtcga tagattcatt aagcataaac ataaaataaa cgtcccgatt
     5401 aacccaatca cactggagct gacagcaatc agtacgctgt taacaaaggc ctgtgtgtaa
     5461 tatttgttgc tgaaaatatc actgtaattg gccaatgtcc actggttagt taaatcactg
     5521 tgcaagctgc acagaaccat aaaaagcagc ggcagcagta aaaatgcaca gaagataaag
     5581 gaggcaggaa ccagaaccaa gcgttttttc atctctttca tcccctcatc aattcttgaa
     5641 tgtcctgcat tttctctgac ggtgcaattc ctaataacca gcaaaacaat ggagccgcca
     5701 gcagctgtgg tacagtttgt tccttcagct tttggcgttt atttgaaatc aaataaaaag
     5761 gaacttcacg atgtgcagcc aagctgccgc catgcagtcc gtgcgcatcc atcccgtgat
     5821 ccgcagtcac cacgacttga taaccttttt gcagccattc aggaaggtaa atagccaata
     5881 atctgtccgc ctgattaacc gctgctgcat attcatgaga atcggcggta aatttgtgac
     5941 cgacatcatc aatattcatc gaatgtacga ccaaaaagtc tggctgatac tgattcacta
     6001 aataatgggc atccgcaaat aaatgcgaat cagggtaagt gtcctcacta taaaagatgc
     6061 catgctgaat cagtcgctcc tcctgatgct ggatgcgatc aaccaaacga ttataggggg
     6121 cttgattgta caactcgctg taccagtggt aagcggcggc tcctgttttt ccgccagctt
     6181 ccttaactaa ttcaaacagc gaccactctt tggatcgctg agcatggtaa ttactataga
     6241 tgccattttc ataggctgga acgccggtca tcagtacttc gtataacgga cgtgaattgc
     6301 tgggcagctc actttttacc ttcaataagc ttgcttgctg gtgctcaact aaatgattca
     6361 tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc gtctagtata actacaatca
     6421 gtttgtcaag tctactttgc ataggtcagt acctccgcct gccaaagctc tggaagctct
     6481 acagaggtct tgttccaagc ttcattgtct tcaactggca ccgccgcttc ataagcatcc
     6541 tccggcagta atttttcagc tacttcttta ggcagctcta ccttgcgaat aggtcgcgca
     6601 tagccctttg ccaaattgat ttgcccttca tcagataaaa tatattcccg tgctagcttt
     6661 gccgcattgg gatgaggggc atttttattg attaaggttg tatacccgct aatgatggag
     6721 ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa tttggtcacg ataatttagt
     6781 ccattgaagt cccaaacaac tgccacatcg atttcacctt tttcaagatt tgctacgctg
     6841 gcatcaacgg tggacaaacg gcctttttct gccatttttt tgtagtaatt cagacctggc
     6901 tgaatatcct tttcgtcacc tccgttggca atcgccgcag ccaatacagc aaattgtgct
     6961 tggttggcaa tcgtcacatc ccccacgcta acttgatagt caccctcttg cagctccttc
     7021 cagctagtcg gtgcttgctt cacattctct ttatcggtga taaaggaaat ggttcccgta
     7081 tagcttaaca accaatcccc atcatcatcc tttgcccaat ccggaatttc ctcccaatag
     7141 ttggtcttat aaggtaaggt taactctttg ctttcagcca acggaccgaa gctgataccg
     7201 acatcaccaa tatcggcggt tccatttttt ccttctgatt caaacttagc caattcttct
     7261 gcactggaca tatccgtatc cacgtgactt aagccgtatt ctttttcaag gtcgttccaa
     7321 gtgcctaccc aatttgccca agtatccggc attcccaagc tggcaatctc tccttcttcc
     7381 ttggcttttt ctataatcgt atctaaagag tcgtctgccg cagccgattg cttcgttgct
     7441 tcacctgaga cgcagcctgt cagcaggata cttactgttg ctgcacaaaa taaaccgata
     7501 gtcatgttac gaattttttc attcattttc attcattctt ctcccctttc gataattcca
     7561 ttctaacaaa gctttgtaat gagagaatca gtttcttgaa aaggtttcat gaacaatgta
     7621 aaggttgtat atggtttgta aatttcgacg aattcccgtt aaccttctaa agcttttgcg
     7681 gtatgatagt agaaaaaagg aggctcccta tgcagtatta tgatcagcat ctccacacgt
     7741 atttttcctt tgattcagag gaaaaatttg aaaactattt agcctatcag ccggagtttt
     7801 tcgtttctac ggatcacttt gatttaaaca atccatctac cggaaaagat gacattccgg
     7861 attatccagc ctatataaac aaactggact ctttaaaaga aacctattcc accaaatttc
     7921 tgcgaggtat cgagattggc gttgttccag gacaagaaga gcggatcgtg gattacctcg
     7981 ctcagcatcc ttatgattta aagctggtaa gtattcatca aaatggccgc tttgattata
     8041 tggacgatgt ggttttaacc aaagatccca ttcaaactac caaggaatat tttgatcaga
     8101 tgttgcaggt cttgacggat tttccagaag gagatatttt gactcacttt gattatggat
     8161 tacgccgctt caccttcaca gcggaagaat tcgcggagca ttttgaggct cagctgctaa
     8221 cgattttcaa aaaggttgtt gaacgagaat tggcgatgga gttaaatgcc aagagcttct
     8281 tgaaatacgg caacgaagac ctttaccgtt atgccgttcc tttgtatcaa agtgtcggcg
     8341 gaaagctctt taccttaggc tcggatgctc atgttgccga agattatcag ctgggcttta
     8401 aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa gttgctagtc attcaaggaa
     8461 aagaacgccg cctgacgccg ctgccgattt tgtgattcct atataataga agaaagtggt
     8521 gaaaaaaatg agcaacctgt gggactacaa tcaagaggca cccatccatt atttaattgc
     8581 gcgacattgg gatgcgttga aaattgaggc ggtttgccga tccttgttag cagccgttcc
     8641 aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg cagcgggaaa aagttcaggc
     8701 ttattttgca gcctttaaag atcagccttt ggaatatttg catgcgcagt ttcatctttt
     8761 ttatcaagta gccgccccag atgactacaa cgatttacga ggacagctgc aattgacctt
     8821 tcaagcggat gaaaccgcct ataccgtttt attgggaatg gctcgccttg gtgatcaggc
     8881 gaaggtcgag tggcggattt tcgatatttg atggcaggat caaggcgttt cagttgcaaa
     8941 ccttcctctc tttcaattag aaaaaccgca gcaggaagaa gtttgtgtgc tggtcaccga
     9001 tgctggtgaa attcgtctcc gcttatttcc tgaccaagct cccctggctg tagccaactg
     9061 gcgctcccta gctgagcaag gcgtttatga caacacacct ttcggctcgt gtcattaagg
     9121 aatttgttat tcaaggtggc gctttggatg gttctggtga tgaagctgtt tccagctatg
     9181 agggcttttt cgctgatgaa gtgcatcgcg gactttatca ttttaatggc gccttggcct
     9241 taggggacca cggcccccac accaacggca accagttttt tattgtgcaa aacacaaagg
     9301 cgcaggctga cctgctaatg taaagcaagc atacttggag cttggaggac taccggagct
     9361 ggatggtcgt tatactgttt ttggacaggt ataccaaggg ttagaaatcg tagcagcgat
     9421 cgcagctcag ccgacaaaca gtgaagatgt accgattgag ccagtgttca ttcgacaaat
     9481 taacttcgaa aaaacatcgt aaaaagacac tcactgactt cagcaaaagc tctattcttg
     9541 agctggctgc agctagtgag tgtctttcat ttatccataa gataaagctt ggattcaata
     9601 aataaataaa tgttttggtt aaagctgtga ccagcaaatg aggaaccgtg cgagccagtt
     9661 gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg cgtgtctgcc aacggatact
     9721 ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg gtacggtagt aattctaacg
     9781 gtacgaaccg atcaagatct gccacaaaac aagcaatcgt cagcagctcg gtcaggttca
     9841 tattgatacc tggaaccaca ggaactcgaa ttcgcacttc gcagttagga tactcaatta
     9901 aaaagcgacg aatattcttc aaaatttgtt ggttatctaa gccggtccat tgtttgtgga
     9961 gcttgctggt aaacattttt acatccacaa acaatgtatc cacataggga gctacctttt
    10021 ggatttcatt ataagcacca caaaagctgg tttccagtac ggtattgatt ccaatgtatt
    10081 tcgatttttg caaaatttct ttagcaaaat ccgcctgaac cagtgcctcg cctccgctaa
    10141 tggtcactcc gccatccgag tgaaagtaaa aaacagcatc cttctcaatc tcatccatta
    10201 cctcttcagc ggtcatggtc tggccaaatc ctttttcaat ctgcttgctt tgtgattcgg
    10261 gaatcgaaca ccaggcacag cgtaatgggc agccctttaa ataaacaacg gtccgtaaac
    10321 cagcaccatc atgtagagaa ccctgttcta ttcttaatac tgttccttcc atatagattt
    10381 ttgaattaca cactagttta aactaaaaca cacaaggaaa aacttaccat ttaatcataa
    10441 aattagctca tataaatata ctaggatgta aaatagactg gtcaaaatct ggtcaaaaaa
    10501 gaggactaat acaggtagtc ctcttttttg ttattttaat attctaaatc attttttatt
    10561 ggtttattcc cttattcgat gtagactcaa atcactatat cactagatat caaacggcgt
    10621 catacggaat caaaaacatc aaatttgacc tgttaaatgg tcattgaata acattcaaaa
    10681 tcaataaaac ggagtcagac acggagtcaa aaacgctgag agtaaaaaat atatacagcc
    10741 ttgtaggtaa agtaccacag ggctgtatat tttttatttc agattacctt aaatgattcc
    10801 atttactttt tatctcattt tttcctacta gttagtatca aatactctcc tattttctca
    10861 ataaactatt tttggataca tggtatctga aacagctaaa accttgtcat tacagcaaaa
    10921 aatcacgtt
//
LOCUS       DACEMK010000091        10652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEMK010000091 DACEMK010000000
VERSION     DACEMK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.094"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            87..305
                     /locus_tag="IW715_002154"
     CDS             87..305
                     /locus_tag="IW715_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022749.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(594..1106)
                     /locus_tag="IW715_002155"
     CDS             complement(594..1106)
                     /locus_tag="IW715_002155"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1022750.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="IW715_002156"
     CDS             complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="IW715_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1022751.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3559..3993)
                     /locus_tag="IW715_002157"
     CDS             complement(3559..3993)
                     /locus_tag="IW715_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1022752.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4003..4791)
                     /locus_tag="IW715_002158"
     CDS             complement(4003..4791)
                     /locus_tag="IW715_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1022753.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4788..5729)
                     /locus_tag="IW715_002159"
     CDS             complement(4788..5729)
                     /locus_tag="IW715_002159"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1022754.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5906..6493)
                     /locus_tag="IW715_002160"
     CDS             complement(5906..6493)
                     /locus_tag="IW715_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1022755.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6714..8027)
                     /gene="obgE"
                     /locus_tag="IW715_002161"
     CDS             complement(6714..8027)
                     /gene="obgE"
                     /locus_tag="IW715_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1022756.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8315..9325
                     /gene="gap"
                     /locus_tag="IW715_002162"
     CDS             8315..9325
                     /gene="gap"
                     /locus_tag="IW715_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1022757.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9410..10345)
                     /locus_tag="IW715_002163"
     CDS             complement(9410..10345)
                     /locus_tag="IW715_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1022758.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10531..>10652
                     /locus_tag="IW715_002164"
     CDS             10531..>10652
                     /locus_tag="IW715_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1022759.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         2979 a         2393 c         1844 g         3436 t
ORIGIN      
        1 atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt
       61 cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa
      121 ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt
      181 ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg
      241 agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat
      301 cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg
      361 tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg
      421 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
      481 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
      541 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
      601 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
      661 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
      721 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
      781 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
      841 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
      901 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
      961 attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca
     1021 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     1081 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     1141 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg
     1201 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     1261 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     1321 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     1381 ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     1441 caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg
     1501 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg
     1561 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     1621 ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc
     1681 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     1741 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     1801 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     1861 accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg
     1921 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     1981 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     2041 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     2101 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     2161 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     2221 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     2281 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     2341 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     2401 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     2461 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     2521 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     2581 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     2641 cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat
     2701 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     2761 aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     2821 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     2881 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     2941 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     3001 gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa
     3061 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     3121 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     3181 gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc
     3241 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     3301 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     3361 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     3421 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     3481 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     3541 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     3601 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     3661 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     3721 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     3781 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     3841 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     3901 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     3961 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     4021 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     4081 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     4141 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     4201 atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac
     4261 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     4321 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat
     4381 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     4441 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     4501 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     4561 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     4621 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     4681 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     4741 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     4801 tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac
     4861 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     4921 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     4981 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     5041 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     5101 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     5161 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     5221 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     5281 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     5341 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     5401 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     5461 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     5521 aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata
     5581 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     5641 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     5701 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     5761 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     5821 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     5881 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     5941 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     6001 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc
     6061 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     6121 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     6181 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     6241 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     6301 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
     6361 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
     6421 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
     6481 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
     6541 tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
     6601 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
     6661 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
     6721 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
     6781 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
     6841 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
     6901 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
     6961 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
     7021 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
     7081 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
     7141 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
     7201 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
     7261 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
     7321 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
     7381 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt
     7441 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
     7501 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
     7561 tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga
     7621 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
     7681 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
     7741 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
     7801 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
     7861 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
     7921 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
     7981 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
     8041 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
     8101 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
     8161 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
     8221 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
     8281 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
     8341 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
     8401 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
     8461 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
     8521 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
     8581 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
     8641 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
     8701 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
     8761 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
     8821 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
     8881 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
     8941 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
     9001 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
     9061 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
     9121 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
     9181 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
     9241 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
     9301 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
     9361 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
     9421 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
     9481 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
     9541 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
     9601 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
     9661 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
     9721 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
     9781 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
     9841 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
     9901 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
     9961 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    10021 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    10081 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    10141 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    10201 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    10261 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    10321 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    10381 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aacccgcgaa
    10441 ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat tgtatttccg
    10501 acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac catagacgaa
    10561 ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga
    10621 atgggacgtg ctatacaaac tatttataat gt
//
LOCUS       DACEMK010000092        10415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEMK010000092 DACEMK010000000
VERSION     DACEMK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.095"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IW715_002165"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IW715_002165"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1022760.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IW715_002166"
     CDS             1007..1927
                     /locus_tag="IW715_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1022761.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IW715_002167"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IW715_002167"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1022762.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IW715_002168"
     CDS             3233..4711
                     /locus_tag="IW715_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1022763.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IW715_002169"
     CDS             4988..7048
                     /locus_tag="IW715_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022764.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IW715_002170"
     CDS             7131..7700
                     /locus_tag="IW715_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1022765.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IW715_002171"
     CDS             complement(7875..8681)
                     /locus_tag="IW715_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022766.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IW715_002172"
     CDS             8819..10333
                     /locus_tag="IW715_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1022767.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3694 a         1739 c         2049 g         2933 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagtg
//
LOCUS       DACEMK010000093         9873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEMK010000093 DACEMK010000000
VERSION     DACEMK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.096"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(22..267)
                     /locus_tag="IW715_002173"
     CDS             complement(22..267)
                     /locus_tag="IW715_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022768.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="IW715_002174"
     CDS             complement(381..959)
                     /locus_tag="IW715_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1022769.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="IW715_002175"
     CDS             complement(1036..1566)
                     /locus_tag="IW715_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1022770.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IW715_002176"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IW715_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1022771.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="IW715_002177"
     CDS             complement(2423..2818)
                     /locus_tag="IW715_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1022772.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="IW715_002178"
     CDS             complement(2815..4227)
                     /locus_tag="IW715_002178"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1022773.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IW715_002179"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IW715_002179"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1022774.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="IW715_002180"
     CDS             complement(5082..6542)
                     /locus_tag="IW715_002180"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1022775.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="IW715_002181"
     CDS             complement(6712..7869)
                     /locus_tag="IW715_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1022776.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IW715_002182"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IW715_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1022777.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="IW715_002183"
     CDS             complement(9336..9863)
                     /locus_tag="IW715_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1022778.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DACEMK010000094         9717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEMK010000094 DACEMK010000000
VERSION     DACEMK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.097"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            170..1561
                     /locus_tag="IW715_002184"
     CDS             170..1561
                     /locus_tag="IW715_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1022779.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1634..2281)
                     /locus_tag="IW715_002185"
     CDS             complement(1634..2281)
                     /locus_tag="IW715_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1022780.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2289..2975)
                     /locus_tag="IW715_002186"
     CDS             complement(2289..2975)
                     /locus_tag="IW715_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022781.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2981..4054)
                     /locus_tag="IW715_002187"
     CDS             complement(2981..4054)
                     /locus_tag="IW715_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1022782.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4276..4950
                     /locus_tag="IW715_002188"
     CDS             4276..4950
                     /locus_tag="IW715_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1022783.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4955..6295
                     /locus_tag="IW715_002189"
     CDS             4955..6295
                     /locus_tag="IW715_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1022784.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6444..7430)
                     /locus_tag="IW715_002190"
     CDS             complement(6444..7430)
                     /locus_tag="IW715_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1022785.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            8154..9185
                     /locus_tag="IW715_002191"
     CDS             8154..9185
                     /locus_tag="IW715_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1022786.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            9294..>9717
                     /locus_tag="IW715_002192"
     CDS             9294..>9717
                     /locus_tag="IW715_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1022787.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         3123 a         1756 c         1672 g         3166 t
ORIGIN      
        1 taatatgata attattatac gaagggcgct aaagggaatc acaccaattt tcataccaat
       61 tggtttcatt tatgaagttg attccttcga ttaatgagcg tttcattgaa agaaaagggt
      121 tgagggagaa aaacatatga tcaggttgtt ggaacgaaac tatagaaaga tggaaaaaat
      181 cattcgaatt ctgtccattt ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat
      241 tggtagttca gaatctacat ttgtaaggta tatcgaagag atcaaaatcc gctggggaac
      301 ggatgtaacg attcaatctt cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc
      361 agcttattta aatgttctga tggaaatgtc gcaaagttct acaaacagtc gtttattaaa
      421 tgagattatt gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag
      481 ccggtcttct tttacacgta agttgaaaca atgcaatcaa attttagaaa gctatttatt
      541 aaaagtgatc gttgaccaag gttttcagtt aactagcctt aaaagtgagc tccagttgcg
      601 tatctttgtt acctttttct ttttaagtta ctacggaaga cgcacgttgc catttccaat
      661 cgaaaaaaag aagatcaaag aaattatggt caagaataat tgtcagatga atttgtttca
      721 ggaagaaagc ccatatgagc aatctttctt tattatgtat tttgttgtgc atctcatgag
      781 agaatcccaa ggaactccat ttgaatggaa agctggagac tcttatgtac agttgataaa
      841 tatcgataaa aaagacttct taatcttgaa aaatctctta ggaaaattga agtacagcag
      901 ctatataaaa gtagtgaact attttgtcaa tacgattttt tcagaatttt cggagaaaga
      961 aaaaaatgaa atcagtgagc agatacgaaa aaatatgtcc tactgttcgt tttcatccgc
     1021 tgattgcaaa gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa tttatttttt
     1081 ctcccgtatt ttcccatacg atacgactca tttgacgatc cgtgcaactt actttgcaca
     1141 gcagatgcaa caccgccaga tacgtctata tgctggattt cgtgcagaac ttcaatttta
     1201 ttccactcta ctgaatcaga atctgattta ttatttatct agctatatct tttggatgct
     1261 tatcggtaat cctgaaattc accattatac ttctgataaa aaaatactga ttatcagcga
     1321 cttaagtctt cgacatagtc aatatattga agaatatatc agtgatattc ttgctgtgca
     1381 taaaattcac tctgagacaa ctgctattac tgaagatcag ctgtcgaaat acaatcttct
     1441 cgagtatgat ctgatcgtta cgaatcaacc cattttgaat gcccatgttc ctcatatatt
     1501 gattgatgat agtgtgtcat tcgcaaatga agaagaactc atccgcttat tcgagttatg
     1561 aataatcaaa aaacagctat gtataaacca ccctgataaa aatttcagga caaatacaca
     1621 tagctgtttc tttttattgt attccttcag caatccaacg agatttatag cgaaagtctt
     1681 ttaacaactc ttcttttgtc caattatttg caatgagatc cgtcaaagtg ttcattacga
     1741 gggtcatgga aaagtatagg aaactaactg cttcttccga cgattgaatg gagaaatgat
     1801 tgattcttaa taattccagc catttttcgt tcattggaga atcagccgat agttctctac
     1861 gtttggttgt ctgctgatca tttcgctggg ttaacaagta taacctttgc caatagatac
     1921 tttcgtgatc taagtcgcag caccactcta aatatttttc gatccccaaa aagaaatgtt
     1981 gtttgttttc gctaatgaat tttaagatat cttgatggac tatgtgagaa ctgcttttga
     2041 tcgtatacaa ataagcatct tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca
     2101 tccccatctt gtcaacgatt tctgagactt tgacttggct gatgtgctgt gagcaaaata
     2161 tctccagtaa taggtcatcg attctctgtt tctttgcgtt cgataaattg aaaaaagttt
     2221 cttttggcat ccacatctct cctttatctc tgattatacg tggatagccc ttcattgtca
     2281 tgaaggattc attccttctt tttctcttct tctgtcaagt acccatcttc cattcgataa
     2341 acgcgatcac tccattgaat catacggaca tcatgagtaa ccataatagt cgctttttgt
     2401 ttctcatgag cttctttaac tagtaatttg acgacttcat atgcatgatc tgtatccaaa
     2461 ctagctgttg gttcgtctgc taagatcaag cttggctcat tatataaagc gcgagcaatt
     2521 gccactctct gtctttctcc accagaaagg tctttaggat aactattttt aagtgactgg
     2581 atatctaaag attgcagcaa ggaatcgatt tttgttgaat cttctttttt tgaagtaact
     2641 tggtcaacta gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt agagctttgg
     2701 agaataaatc ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt
     2761 agtgagtgac cgttgatcac tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg
     2821 gtaaggaaag tacttttacc tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc
     2881 tttacataaa aatcaattgc tttcaaagct gagacctttg tatgaccaga accaaatgtt
     2941 ttggtaactt ttttcatcgt taatattttt ttcattttta ttaacctcct attgctgtaa
     3001 ttgggtctac tttggttatc gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga
     3061 tcaaaatccc actatatagc aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg
     3121 gtaaaatcag actagtcaaa taagcaaaca aaagagcaag cagaacgcct accaccccaa
     3181 caataaagga ctgtgctact aaagatttag cgatgaagaa atttttgatc ccttgtgctt
     3241 tcattactcc aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca
     3301 cggcagcaac cactaagaac aaaaagtata tcatcgcatt taaggtcata ttttgggcag
     3361 aatagccagg aatactttca atgaatgtcc caatagttaa taactgtaga cccttcgatg
     3421 ctttttcttt cgaaatttta gctgaatcct ttattacgat cccattgatc ggctgttctt
     3481 cttcagaagc aaacggctgt tgtccatact tcaaagcagt ccacgtatct agggacgcat
     3541 agatgacggg agaaactgtg taataagttt caggaaagat accgacaatc gtcaaaggat
     3601 cgtcgtaact gccaattttg atcgtatctc caattttata gcctgaatca gccagatttt
     3661 gagagatgat gatttcattc tttgttttaa attcatgccc tttcgtgagt tttggtaata
     3721 aaaatgcttc tttcgttgtg ccgaaaactg taacattttc tttgtttttt cctttgattg
     3781 ctccgctgta caatccgatt ggtgctttct ctcctccttc aatgtatttc agatctcctc
     3841 ttgttaattg tgaagctgca aatacttgat ttgcatcctc cgacaaaaca atctcttgtg
     3901 cgtcccaatc atcgattgct tttttaaact cttctgaaag tccattcgct agtcctgaca
     3961 acatgaatac gacatacgca atcaagaaca taataccgac gatcaagcca tatcgtaatt
     4021 tagaatagcg aatttctttg atagctagaa acatttgttt ttcctcccta tgatagattg
     4081 atagtgacaa aatgtcactt gttgaatagt gtaactctaa agtgacaatt tgtcacttgt
     4141 gttgtttttt tcttatcaat aaattgtcta agacatcttt cgataaaacc ttaacatgag
     4201 tcataaaaaa tttatttaag ccttatttat gctttccatg ataaactaaa gtgaccaaat
     4261 caatgaggtg ggaaaatgat taaactatta gtagtagaag acgataaagt attatccaat
     4321 aatataaaag aaatactcgc tgcaatcggc gagataacac aagtttatga tggtagtgaa
     4381 ggactgttcg agttggaaag tggagtttat gatcttgcaa tcttggattt gatgatgccg
     4441 gaaatgaatg gatatgaagt gttagcaaaa atacggaaag aaaaaataca gactccagta
     4501 ttgattttga ctgcaaaaga tagtttggaa gataagattg aaggatttga acgaggtgca
     4561 gatgattatc taacaaaacc tttctatcga gaagaactga tcatgcgcgt gaaagcatta
     4621 ttgaaacgct cgctaggtct ctttgatgaa aatgttctga agtataagga tatcagttgt
     4681 gatttaaaaa gcaatgaagt cttgtataaa gaacaagttt tgcccataca aggaaaagag
     4741 tttgaattgc tcgtttattt tttacaaaat aagggaatca ttttgacaaa agagcagata
     4801 tttgaccgga tctggggatt tgattctgat acgacgatta cagttattga agtttatatg
     4861 agcaacttac gaaaacatct gcgaagtact ggattggata aagaaatcaa aaccttacgt
     4921 aatgtcggtt atatactgca aagggtgtga tgcaatgaat ggagatatta cacgaaagca
     4981 acgtagtcgc ttttttgcga caaatgtagc agcatttgct tttatctttt ttcttttggg
     5041 attcattact ttacagatat taaaccaatc tgcatacaga gaaacagatc tgtcattaga
     5101 gaaaataaat tttgattctc gaacagtaca attagagata caaagatatc agcaaggcga
     5161 tcccttttta aacccgactc aaacggaaca aaacagaaca aacatggaac agccattaat
     5221 ggaaccatca aatgaccgtt ttaacactca agtgatcttg tggtctaaaa atggtgagat
     5281 attgaataaa gcagctattg gtgggcgttt caaccaaatc aaagaattaa aaatggatac
     5341 gaaaaatctg aataccatcc aaaatattga attaaatgaa gacagtggct catctgccct
     5401 ctctttccgt tcgattacga aaaaagcgga gacgaatcaa gatggcgttg cctacatcca
     5461 aattttagaa aatacaaatc aaatcaagaa ttcattagcg acttttcgca cgattatgat
     5521 tttgtgtatg attgtttttt ggattctctc tattggtatc agttattact tgtctagtct
     5581 aagcatgcgc cctattattc tttcttggag aagacaaaaa gaatttgttg agaatgcttc
     5641 tcatgaattg cgcacaccgt taacgattat tcaaaatagt ttggaacatc tttttaccaa
     5701 gcctaatcat actattattg aagaatccga aagcatcgct caagcattgt cagagacgag
     5761 gcgcctcact ggattgactt cggatttgct gacaatcgct cggaatgatt ctgatcagca
     5821 gctgctttca aagcaaatga tcaatacaca agaatatatc gaacaacttg ttaaaccatt
     5881 tcaagagatg gccatcatag atggaaaaga gtttattctc cataacgatg caacattaga
     5941 agtaccaatt gatgaaaaaa aaatccatca agttttggtt attttgctgg ataatgcatt
     6001 aaaatatacg aatccagaag ataagatcat tttggattca tctgttaacc ataaagaatg
     6061 gctgattcac gtaaagaata ctggcccttc tatcagtgaa gaagatcaag caaggatttt
     6121 tgatcgattt tatcgggaag atcaatctcg ggcgaaagag actggcggat acgggcttgg
     6181 cttggcaatt gcaaaacagg taatcgaaca gcatcatgga cagatttctg tatccaatta
     6241 tcttccacaa ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa aataatgctt
     6301 atccctaaaa aagggctgtg acaaaaatct tgtcacagcc cttttttgtt gcgaataaac
     6361 ggtgttccag aagtagcttc ttcggaaata agccgaaatt tctttaagct gaccacttct
     6421 gtcacaacct ctttagctta cgcttacgat tttttattgc ggtcttcaat gtgtacatca
     6481 ccgcttgtgt caatatccaa ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct
     6541 tcatgttctt ctttatgaat cccaacttct tcacgaataa ctgtatgttt gcttacatct
     6601 acttgctctt ctttgattgg gatagtaatc gtgtcatctt ccaatttttc gtctgttccc
     6661 tgtgaagatt gatctgttac tggtttgcgt tcgatgacga tttcttcacg tttgaccggt
     6721 acttctaccg tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc
     6781 acatcatgtg tatttacatc taatttttct tcttttagtt gaattgtatc atcatctaga
     6841 tttggtttgc gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt
     6901 acgtccatcg gatcagttcc tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct
     6961 gtcgtgccac ctacgtccat tggatctgtt ccagtgccag tttctaatcc agtccccatt
     7021 gtgcttgctg ggctaacagt atcttgtgta tccattgtat cttctgttgt ttctggctga
     7081 cgatagcctt ttactgtgat gacaaatttt ccatcagaaa tatctcctct atattgatat
     7141 aaaggatcat tttctggtgt agccgtctca gaatcatagg tatcaaaaga aaaagtatct
     7201 tttatttttt cccataaaga acggtcttct tcaccatcca tccgattttc atctgtttca
     7261 acatctatac cagacaatcc gtcaaatcct agtctagatg ctgcttcatc agtagtataa
     7321 ataacgatat catctcgttc atacccttcg ttacgcaacc gttcaattac tgataatgct
     7381 tcttccctcg tactatagct tccgatgacc gttaaatttt tctcttccat gtaaattcct
     7441 cctagcaaat agatttatga ttggtatatt gttacttata aatttattct ataggagacg
     7501 attgttttct acaaatattt agattggaat ttgattaaaa caatttaatt gaaatttgaa
     7561 aacatttatg ttaatgaaag tcatattcta ataaaatgtc atttgtttct atatgaattt
     7621 ttatgaaaaa tttcatataa tttccactcg ttgtgatggt ttaatttatc caatccatca
     7681 ataagcaaac aacttatata aaaaaggatt aattctcaat ataattgctc ctaccataga
     7741 ataaatcttc atgtaaagta atgaagtacc aactatttct aatacttctt taagaattat
     7801 cccaacaata tcgttagaca aaccattatt gaataaatgg agagccaatc gattctgaaa
     7861 aaatgttttc taatttttcg gaaataaaag aaagagcctc ctgccattag aacgaaccaa
     7921 atccatttca ttgctcatga agtaaccaca tagctaaaaa gaggataaat caattaaaag
     7981 atcaaaagaa tgactgcact aataaaataa ctaaaaatgt atcttcttac ttcatatctt
     8041 ttagctagta cttagttaaa aatttgcttc ctaagatcaa gacaaactgt ctatcttcat
     8101 ttgatgaaat gcggtaaaat atagaaaatg aacactattg gagggaattt gtaatggaca
     8161 gcaaaactag aaaacaactg caagaaaagt taaagcaacg tacacacttg ctcccttccg
     8221 tctttcaaca gtttttagaa gaaaatcgtt cttccctttc attaggcagt cgctatgaat
     8281 acgcaaaaga ttttcatctg tttactgatt atttgagaaa agtattttct gaagaagtgg
     8341 atgatcagct gattatccat ttagaaaaac aagattatca gaatttctta gctaaaatct
     8401 atcgacatac tcgttcgttc caaactacag cgaaccaaga aacggagcaa ttgtttgaaa
     8461 atagctatta cgggatttca aggaagcaat attcattaaa tcatttttta cgatttttgt
     8521 atcaaaaagg actgattgaa gaagagattt ctcttttggg tacaagttta ccggaaacaa
     8581 ccaaagcagc tccgcgatat atcgatgcag aacttttcaa agattttgag catttgttta
     8641 tcccgattga aggctttcaa acaagcagag aagctagctt tgaagaaaag aatagaccga
     8701 ggaaccttgc aattactgcc attcttctgt attgcggatt aaagatccat gaagtagtag
     8761 aacttaaacg taaagatgta tctttgacca agcagattct taatctgaat agaaaagaaa
     8821 acagactgaa taaagtacca ataccaaaag aagcgatgcc atttctggag aactatttta
     8881 agttaacagc acaaaatacg gacgaaaatt cttttgtttt tcgttcctca catgatcagc
     8941 aaatttctcc tcggactatt cgccaaattt tgagcaaaat tacagtatat aaaaacgtat
     9001 cgccagaact atgcagaaaa acatatcgtt attatgcaga actatattta gatgatgctg
     9061 acgcggtaaa ttatttatgt ggtaatcgat acctctcatc ccattctttt caagatatct
     9121 gggaaaagat ggttgatttt tcttatcacg aattagcggc attcgttcgg gtttcttcca
     9181 tataaaacaa atgtatgatc tttttttcac aataaactat gttattatat aaaggattat
     9241 gttacgatta tcttgaattt gtatattact taacaaaaga taatggagtg tgaatgatgg
     9301 agaccataac gtattcccct ttgaacttat ttacgaattt tcaagaagct gcagctaact
     9361 tttccgagac cccgattatc tttgatgagc cactttcagc tttcccagaa ttaggacttg
     9421 aaacaactta caaaaaatgc tgcgaaagtg tgttgaaacg ggcttatcaa ttggctcatc
     9481 ttggagtgaa atccggagac aaagttatta tttataagag ttccgctttt gacacatacc
     9541 ttctagctgt ttctgtgtct taccttggtg cagtgcctgt catgacttct tatcatctac
     9601 caacaacaac aatggaagta tttatcgatc gtttagaaga ccctttcatt ttatttgatg
     9661 atgagacaaa aaagcgagtg agagaaattt caaatggaac aaaatcaaaa cagattc
//
LOCUS       DACEMK010000095         9453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEMK010000095 DACEMK010000000
VERSION     DACEMK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.098"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            226..1032
                     /locus_tag="IW715_002193"
     CDS             226..1032
                     /locus_tag="IW715_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1022788.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1044..2264
                     /locus_tag="IW715_002194"
     CDS             1044..2264
                     /locus_tag="IW715_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022789.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2254..3840
                     /locus_tag="IW715_002195"
     CDS             2254..3840
                     /locus_tag="IW715_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1022790.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3879..6017
                     /locus_tag="IW715_002196"
     CDS             3879..6017
                     /locus_tag="IW715_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1022791.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6305..7696
                     /locus_tag="IW715_002197"
     CDS             6305..7696
                     /locus_tag="IW715_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1022792.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7755..8564
                     /locus_tag="IW715_002198"
     CDS             7755..8564
                     /locus_tag="IW715_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1022793.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8536..>9453
                     /locus_tag="IW715_002199"
     CDS             8536..>9453
                     /locus_tag="IW715_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1022794.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKK"
BASE COUNT         3270 a         1446 c         1902 g         2835 t
ORIGIN      
        1 aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta tagaacccct
       61 tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct
      121 tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa
      181 atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt taaagatttg
      241 tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt ttcgatcaat
      301 gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt tatcaatccg
      361 ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc acaaatgatc
      421 gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg gttcttcttc
      481 tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt agtaaagaaa
      541 gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct gtacacccat
      601 ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata tccttcagca
      661 atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac aggattgact
      721 tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat tacgatcgtg
      781 ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt tgattcaggt
      841 cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta tcgtgagtca
      901 tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt ctgggttttc
      961 acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc acatttttca
     1021 gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa gatatcacaa
     1081 aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc aatcctttta
     1141 agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa ataaaaaaag
     1201 gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg ttgaaaatca
     1261 ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa atctcagctt
     1321 tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat atctatttaa
     1381 atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat gatatcattg
     1441 aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca agtgggatgt
     1501 tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg attgtggatg
     1561 aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa tttacagaaa
     1621 ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg attcgccgtt
     1681 tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt gatacgatgg
     1741 aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca gtagatcgaa
     1801 ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg aaagccgaat
     1861 tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa ctatacgtta
     1921 aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga attgctctgc
     1981 gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt atcaagatcc
     2041 cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt ttgttagctg
     2101 gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct ttggtataca
     2161 agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc atcgtggtct
     2221 tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga aatggaccta
     2281 gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag taaagtttta
     2341 gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa acaatgtgaa
     2401 gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt tgcccaagat
     2461 ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg acagacattt
     2521 gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa aacattgaag
     2581 aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc taacttagca
     2641 cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc ttatggtctc
     2701 ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt ttttgaaaaa
     2761 gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga tacagaatta
     2821 aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat gcgaccattt
     2881 ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca aacttccatt
     2941 gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca aacgaaacaa
     3001 gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga gacactaagt
     3061 ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca acctagcttt
     3121 atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc aaatgctgta
     3181 gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga gttcgctcta
     3241 acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat cggggaattg
     3301 acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt cacaaagcag
     3361 ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga ggaaaataca
     3421 agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt ggaaagtgat
     3481 gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa aaaaatacaa
     3541 gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac aaaaatatta
     3601 aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta taagttatca
     3661 gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt caatcaagca
     3721 gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg cgagcaagaa
     3781 cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat tgaaatgtaa
     3841 ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa gaaattgccg
     3901 tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt gtgggctggg
     3961 caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa aatgtgattg
     4021 ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt gatgttaaga
     4081 cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg atcttagatt
     4141 ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa tacccttatg
     4201 atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa gggataaatt
     4261 atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa ttgaaaccag
     4321 gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg aaagagcaaa
     4381 ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg cctgtctata
     4441 acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa acgtatgatc
     4501 actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga aagtgtctgg
     4561 aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa aatggacata
     4621 tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata gcgctcttag
     4681 ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg ctaaacatac
     4741 atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat ggcaatcgct
     4801 ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat aattatatct
     4861 ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc cgtaaaggct
     4921 acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc cctgcagatc
     4981 atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga tcaaccgcaa
     5041 gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg acagatgccc
     5101 tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga ttttatgaga
     5161 ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg aagaatggct
     5221 atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat ccaaattatg
     5281 aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta tttgcagaat
     5341 acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca ttcaattatt
     5401 cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta ttcttgaata
     5461 acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat gcacagtttg
     5521 atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg cagcatgcag
     5581 gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat gatcgaacac
     5641 attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc actgcagctt
     5701 gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa acattagaag
     5761 tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt tacaatgtct
     5821 atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat gaagatacac
     5881 cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag tggccttctt
     5941 atattgctca tgacccattt tataacgata atctaactaa agaaggaatc ggcgattttt
     6001 cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga aaatacagtt
     6061 tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga acacccattc
     6121 agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa tatacatata
     6181 aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt ggttattcac
     6241 taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg ttaggagtgt
     6301 atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta tcatcatgga
     6361 tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc aaggagaagt
     6421 cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca ttatctttat
     6481 tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca gtgatatcgt
     6541 cttcattact gttattggac aggtgctgat agcattgggg atcatggtag ttacttttgc
     6601 tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac ttggcacgat
     6661 attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca gtcgtgataa
     6721 gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa actttcaatc
     6781 taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt acgaaaacac
     6841 aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc cagaagacga
     6901 aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt taaatacaac
     6961 ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg aaagtatcat
     7021 tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc tttttgatat
     7081 catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca cagcgatttt
     7141 ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta cgaaaaatca
     7201 aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag caaaatcagg
     7261 accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat ttatccgtgc
     7321 cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata tgtcgatcgt
     7381 cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa atccttatta
     7441 ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat acggaaaata
     7501 tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa agtattcctt
     7561 actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata agatgtcctc
     7621 acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact tttcacaaga
     7681 gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga acattggaaa
     7741 ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag tatttaaagg
     7801 aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta ttagtttatg
     7861 acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa aaagatcctc
     7921 acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct ttagcaattg
     7981 gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat atcatggaac
     8041 cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata gcgattgttg
     8101 ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga aggattgtac
     8161 ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt aatcatatga
     8221 cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca ttaattggat
     8281 ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg tataatatcc
     8341 aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt ggaggaagta
     8401 aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt ctaggatcac
     8461 taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta ccgaataaaa
     8521 ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc tataatcata
     8581 cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt aacgcaaggt
     8641 tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag tagtgacatt
     8701 ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga aaacgctggt
     8761 ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt aggattttta
     8821 gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga ctaccatagg
     8881 aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga aaatgcaaat
     8941 ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga gcaaatgttt
     9001 agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga attattcaat
     9061 catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta tgaagctttt
     9121 agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta tcaaaataat
     9181 gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt gcaaagaaaa
     9241 atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc caatgctcga
     9301 ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca aaatcagaaa
     9361 gaaaagcaaa aatattataa tcagtctaaa agagaaataa aaagtatata ttcaaaaata
     9421 agaagtaaca atgagatatc aattgctaaa aaa
//
LOCUS       DACEMK010000096         9411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEMK010000096 DACEMK010000000
VERSION     DACEMK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.099"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..551)
                     /locus_tag="IW715_002200"
     CDS             complement(<1..551)
                     /locus_tag="IW715_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1022795.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
     gene            complement(548..967)
                     /locus_tag="IW715_002201"
     CDS             complement(548..967)
                     /locus_tag="IW715_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1022796.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(964..1209)
                     /locus_tag="IW715_002202"
     CDS             complement(964..1209)
                     /locus_tag="IW715_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022797.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(1169..1525)
                     /locus_tag="IW715_002203"
     CDS             complement(1169..1525)
                     /locus_tag="IW715_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1022798.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(1525..1830)
                     /locus_tag="IW715_002204"
     CDS             complement(1525..1830)
                     /locus_tag="IW715_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022799.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(1827..1988)
                     /locus_tag="IW715_002205"
     CDS             complement(1827..1988)
                     /locus_tag="IW715_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1022800.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(1985..2287)
                     /locus_tag="IW715_002206"
     CDS             complement(1985..2287)
                     /locus_tag="IW715_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022801.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(2449..2718)
                     /locus_tag="IW715_002207"
     CDS             complement(2449..2718)
                     /locus_tag="IW715_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022802.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(2730..3479)
                     /locus_tag="IW715_002208"
     CDS             complement(2730..3479)
                     /locus_tag="IW715_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1022803.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(3482..4168)
                     /locus_tag="IW715_002209"
     CDS             complement(3482..4168)
                     /locus_tag="IW715_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022804.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(4174..4845)
                     /locus_tag="IW715_002210"
     CDS             complement(4174..4845)
                     /locus_tag="IW715_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1022805.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(4838..5179)
                     /locus_tag="IW715_002211"
     CDS             complement(4838..5179)
                     /locus_tag="IW715_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022806.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(5357..6055)
                     /locus_tag="IW715_002212"
     CDS             complement(5357..6055)
                     /locus_tag="IW715_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1022807.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6142..6612
                     /locus_tag="IW715_002213"
     CDS             6142..6612
                     /locus_tag="IW715_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022808.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(6655..6834)
                     /locus_tag="IW715_002214"
     CDS             complement(6655..6834)
                     /locus_tag="IW715_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022809.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6821..7159)
                     /locus_tag="IW715_002215"
     CDS             complement(6821..7159)
                     /locus_tag="IW715_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1022810.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(7175..7378)
                     /locus_tag="IW715_002216"
     CDS             complement(7175..7378)
                     /locus_tag="IW715_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1022811.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(7393..8169)
                     /locus_tag="IW715_002217"
     CDS             complement(7393..8169)
                     /locus_tag="IW715_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1022812.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            8468..8788
                     /locus_tag="IW715_002218"
     CDS             8468..8788
                     /locus_tag="IW715_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022813.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            8806..9237
                     /locus_tag="IW715_002219"
     CDS             8806..9237
                     /locus_tag="IW715_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1022814.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         2567 a         1919 c         1440 g         3485 t
ORIGIN      
        1 tctaccacct cttccactgt tccattcacc aacaatgctg cagcatccgc tgttgcttta
       61 tcagtgaaaa gcaatcgatg agatggattg cattcaaaac tataatttac tccatctttt
      121 ccccataaag cacatttagt aagatagttt ccatcggcat tctttattgc ccataacggc
      181 tctttctcaa cctcgtagcc gttgtatagg ctcaatagtg tttcatatga ttgtcttgtt
      241 gcccaagcga gtaattcttc tccttgctct tgcgtcacta tgccatctcg tacaaaccaa
      301 tcacagaaat aatatgaatc accatcttta gatttgatta aatacgctat tttttcagct
      361 tttgtaaaag ggtccgtaga tttctcaagc caaccggcca cgagttgtgg aataactggt
      421 ttctgcggtt cgtctatttt tttcattaac gtaatacctg aatttatact ctcgttatag
      481 caagcagcta ttgcatcatt tcctatcgct ttaatacttt ctaacttttt gattgcttcc
      541 tgtttattca tcgctgttcc tccaataact cgctattctc gtatatattt ccggtgactt
      601 cgtacgtgta ttcttcaaac agctctgtgt taaaaatttg atactctaag tcttcatcaa
      661 cagtggcaca aactaatccg gaataatcta ttgaattttt gacaatacag actttattat
      721 ctaagtaatc gaagccattt cgcacgctga ctaatactat atccccttca aatatatcca
      781 caccattctt atctttcatt cctgtggatt gcatgaggtc gatgtttcgc acgtgatatg
      841 atttcaattc ggtaggattt atccaatatt caataaagtt tgttttgccg tttttagtaa
      901 aatgcaagac ggctacatct ctcattacgt tctttcgttt atcccacgct ctaaacttcg
      961 gtatcattcg ctgtcctcct cgtatttttc aatcaattcc attacttttt tcactatttc
     1021 aaactcaacc gcttttgatt cttcgaaatc atgaacaatt tctggaaaca gtacatcatc
     1081 aactacccac aaaataatct ggcgctttcc accaaaattt atgattagat ggtccgattc
     1141 cacagatagt gttgctcctg attcgatatc atataaatcc atgctgaatt gaacgagttt
     1201 ttttatcatt tgctgtcctc ctcaaatact cttctaatat ttctttatac ttctctacaa
     1261 atttgaaacg atcttgatga agtttcttgc tccaatttgt ttgccgatcc agctcacgca
     1321 tctgatcgaa ccctttttga atttcgttgt aataaaattc aatgtttgct gctgctttcc
     1381 aatgcctgct acttcgcact cctgctcctg tttcagccat ttctaactta actaattcag
     1441 ctcgttcttt tgatttttta tctttctgaa tcttcatcat gattttcttg aggatgatgt
     1501 cactgtattg tgtaatgaga tccattatct ctcctccaca tacctaaact gtcgtccttt
     1561 tgaatcaatc cataagctcc tagctctatc ccagataatg tttttgctta atccagtaat
     1621 ttcagataac tgttcagcag tacctgttac taaaattcgg tcatcatgcc agattgcaat
     1681 ttttctcggc gttctccgtt tgggcttttc agtccacatt gatttaccga gcttttggac
     1741 ttctgcaact atttctttgt cttcttgcca agattctgac ttggttaatt cagcaattct
     1801 tttcattgct gctttcttat ccacgctcat tcctccaatc gatggatttc ccttcttaaa
     1861 ttctctatgt gcaaatcgat tgcctttctc gccgtttcat tgaccatcac tgcctttgtt
     1921 cgttccagat cgtcaatctc acgctgaagg cttcgaatac gcatttgaat cacttcttct
     1981 gttgtcatga tgattcctcc acgtatctaa acgttctctt cttagcgtct gtgtagccac
     2041 accttgctct tttcctcacg attttctcgt gcaagcctgt gagacttgct aactgttcag
     2101 ctgtccctat cactagaagt ttgtcaccgt gccaaattgc gatttttcgt ggtcttggct
     2161 tgttgctctt gtctgcccac atcgctcttc caagcctcat cacttctgaa gcagcttctt
     2221 tgtcattttg ccaatcttct gaataagtca attcgataat tcgctgcatt gccgctttct
     2281 tatccatccc gacattctcc tttcagtaat ttgagtactt gatcaagtgc gctctcacgt
     2341 ccgccatgga acgtgttgag ccacttgtct tcgtacgata cactttgtct taaagcttct
     2401 tgatgcatta gttcaatctg tgctgtaaat gtctttaggt ccatctgatt acacctgctc
     2461 aagttcatta agatgttttt gcaagccttt aacacaatca acaaatagta atttggtata
     2521 tgctaaattt cttaattgtg ttgtatcgat ataaagtgcg aaatagtatc tgagcttact
     2581 ccaactcgaa cgatcattct taattccttc aattccagct tcttcgagtt gatcatatac
     2641 gtctctcagg acgtctaatt cctcgccctt tttataattc gctatttcat taattagttc
     2701 tagataatcg atcttcattt ttccacctct tagaatggtg cttttgattg tctattagct
     2761 cgttctagcg cttttttctt gagataggct tcttggtcga ttgcccattc aggaagcttc
     2821 tctcgtcttc ctgtgcgctt gtatccactg cttgcgttct taggttcact tttttctttc
     2881 cttgcccaac ttcgaatagt tgccaaatag tttttataag tcttaccaga tgattcacaa
     2941 tactctgaca gtcgttcgat tcgctcttgg taatcattag ggaattctgt tttgagtttc
     3001 tccatctgct catctgacaa aagaacattt ttatactctc cgtatttatg acggacgggc
     3061 ttagccttcg attttttcga aggcggtaac tctcttatat attcttttgt attattaaat
     3121 gtattattaa tagatgtatt attatctttg actttttcgt caataggggt attgcgtttt
     3181 tcgtcaatag ggtattgatt aattcgtagg tacctattga ttatttgatt ggtaccctct
     3241 ttgtaaatga tttcccgatt caagtatcca aacttaatca aatcacttac ccatcgcgat
     3301 atggtctctt tattcacacc atataaatct gcaaagtact cattgcctgc ccaacaaaag
     3361 cctctttcat tacacaaggc cgttatctct ccgtataaca acttagtatt tggtttaagt
     3421 cttttgtcgt accttacgtt ggctggtata atcgcataat aacttcgatg ttctgtcatt
     3481 tttaccctcc aatatttaac tttttgattg ttttctggtt taatttgatc cctttgattt
     3541 gatatttatt tttgaaatta atcacaccta ttttgtgctt ctctgtgtga tggattctgc
     3601 agagtgctgc aaatgtgtac tctgcatgat caacttcttt gcgctttcgt cttcctagcg
     3661 ctttgtcaaa gtgatcgatg tcagctcctg ttttgccaca gatgcagcag actctttttg
     3721 tgatgcattt gtagaagtaa tattcttgat tcgcaggtaa aatctcatag ccttctttga
     3781 aaggaatatg atgttcaaag atgaaatcta agatgatatt tgctaagaca ttggcatcac
     3841 tcacagttgt attcgattcg tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac
     3901 gaaagtagaa gaattccttc cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc
     3961 ctatcagcgc atagatgaag tttcgttgct gtacggtgaa acgtctagga tcaataaaac
     4021 gaatttcaat gactcgatca ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt
     4081 tcacttcctc attgatggtt gcgcttatgt cttttccttt gaactttttc agaaccgctg
     4141 aatatgaatc gattaatggt ttaaacactc atatcacttc ttatctaatt cttttctctt
     4201 agctgctatt gctcgctcca tcaaggcaca ttgctcatag cttaactgtt caatagtttc
     4261 aacgttatca gctaagagcc ctaatttatc tgtctgctca ttaacatatt cgattaaggt
     4321 tttggtcata tctttaccca tctgctcatt gaaagcttct agaatcgtct ctagcatgtt
     4381 taatttcttt gtatcgattc taggtggtgt tggaatatct tccccttgaa atacatataa
     4441 tcccagtccg tgtagagcca atgctttcac aaagcatcgc ttcaatgagt tattgatttg
     4501 cattgcattt ggtttaacaa ctggttggtt tcgataatct aaaacaggaa ataattcggt
     4561 ttccgtgtgt cctttaaccg ttactgagac agatacataa gtcccagttt catccataag
     4621 aaaaggttta tattcctcaa caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg
     4681 tttgtactca ttaatagtta ccgttgcctg tggatcattc tttttcataa tctcccacgc
     4741 gtgagcccaa gataaataat caaaatttcc ttttttcttg agaattttat ttaacttacg
     4801 actaaaaagt ttttcaaagt tcgttgtccc tttgatttca ctcatcaaat tctgcctcca
     4861 tttcagcaat gtatttctta cctggtccgt aataagagat atcaatcaag ttatctctgt
     4921 cgtactcttc tagcgcatca atcaagccat cttcgatgac gtaaatatat tcaggtttat
     4981 tcgaatgctt cgatagatgg ataagataaa catgatccca aatagttaca tggttgccta
     5041 aatcatcttg atcccaagct agttcttcat tcgtcaaaag atttcgtctg atttttcgac
     5101 cacttgtttc ctcaattttc ggcttgcccc aatcaggatc aatcaaatat tgatctagag
     5161 tggaaagttc tttttccata tgctaaaatc tccttatgat gtgttttctt tgtgactctt
     5221 tgcttgccgg cggagtcact tttttatttg ttgccatgct ttttgcttgt caatatgttg
     5281 ttggcttagg atgattggtt tatagtattt ccaccagcaa ttagcaattg ccgtccctat
     5341 tcttagcgct tcagctctat tcattgtcat caccaaaaag tctctgttgt ctgttcagtt
     5401 gatcaatttc catacggatc gcagtttctg gcaaccacat ttcaataaat gaaacagcat
     5461 catcgaatct cttacgaggt aactcgccat atcttgggat tgaaaaggta cgtttaaatt
     5521 cagaccaaaa ttttgagaat acttttttgc tgatttcttc ataagctcgg ctttcttttc
     5581 cccctagaac tcccataact ttcatatttc ctttttgctt aatttcaaac tcttgttgtc
     5641 cgctaattcg catagtatct ttaagcatgg aaacatcttt tttaacatct ttcatttctt
     5701 ctaattgata gatcatcata tcttcaattg tttgaggaac agtattcttg cgaataacat
     5761 cttccatttc gttgaatgct tcaatgtatt tttgtttgaa gtagatagct ttctttcctg
     5821 taaaccccat agccagcaag aaaaatccat ctctactaat gaagaaaact cgtcgatttc
     5881 tgccatatga atctggttca ttaccttcta caaacatctg tccaaaattg gacacatctt
     5941 tttttagtgc atcaatatct cttaaaacat gttgatgttt tttctcgaag ctttctgcca
     6001 cttgtaagct cgtagtcaca gcttctttat ttttcaaaat tactaattct tgcattattt
     6061 cttctctcct ttttggtata atttagttaa aaactggtgg tgtttatttt gaattttttt
     6121 cacttaacaa tttttcaatg gatgtcaata ataagtttta tattgagtac gtttttaaca
     6181 gggataaaaa taaaaggaat gcgccctcaa ctagaagttg aacttaatgc ttcttacttc
     6241 gctgcagata tgatttatac aaaagtaatc atttctaact attcaacaga accagctatg
     6301 ttagttaatt tagaactttc atccgctaac ttaaaccgca gatggtcagc tactccattt
     6361 aagaaattaa ttgctaaagg aggatcagtc gatgataata gaatatactc tgaagctgta
     6421 ccgctaaata ttcctcctaa aagtgctata tcttgttacc ttgcatttga agtagggaaa
     6481 attaatttta ataaactcct taatcaccaa acaaaaatga tatttactct aaatagaacc
     6541 caaatccaca aaattgttga tatcaaaaat acaaacttcc caatagagaa attagtgaaa
     6601 gaattaaact gagtaatagt aatatacatt ctagtttctc ttttctattc atttttagtc
     6661 agccccctcg gttggctttt tcgctctgta ctcagcttca gcttcatcaa gccccataaa
     6721 aatccaaacc atgtaaacaa tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa
     6781 agcgtagatg attagcggtg cgctgaacac tagcgctcta ttaaattttc ccatccgctt
     6841 gcctccttaa gatttccgaa aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa
     6901 atagatatgt gtctcttcct tcaactggat aatagacaaa tccatttttg tttttctcga
     6961 tatcgataat atttctatat cttgggttct ttaaaactct agaagtaaac caatcatatt
     7021 ttctgttaat ccgttctagc acttctggca acgtcatcca tctaccagta tcatcagctt
     7081 tttttaactc ctcataatcc acttgggaga taattacata gccttctgga attgggattt
     7141 ttgcttctag atattgcatt agctgttcct ccctttacaa ttcgtacata gtgataatcg
     7201 aatctataat tctgtttgct tctgcagaag tctttttacc gtttaaaatt aaagataagt
     7261 agcttttgct aattccaaat ctttcagcaa gcatggtgta agttaagaac tttgaacttt
     7321 cgacatattc tttgattttt tctctatctc gttgagtgat ttctgcaatg tcagtcatac
     7381 taaaactcct ttctaaccaa tttcctctaa atccatttga gggtaatatc cttctttttt
     7441 tagtaattcg tagataaata gacgcccttt ctgtgtccat ttggtattca ttacaatttt
     7501 agtgccacca tcggctttcg ggatctcagt tgtatgagat tttgtgtatc cttggttcat
     7561 gtgttttttg cacaataacc attggttacc gacttttttc tgaataccta gtttatgaag
     7621 taatttattc atctgttgtg gagacatccc ataatctgct gcaatctgac taattgttac
     7681 tgaatctgta gaagataata tgctatctaa ataggagatt ttaggttcgt actctgcaat
     7741 cttttgttct gcgattaatc ttccagttct ttcttctttt agttgagttg ctaattgaat
     7801 gattgtatct ggattaagca aagcttcttc tactttttct ggagttagat aacctccatg
     7861 ttttctaatt gctggcaaca cttcacttgt tacccaacgt ttaaattttt tggcagaggg
     7921 aagttttgat tttaagatta aactgtacaa accagattcg ttgatgattg tcatctctct
     7981 tgattgacct gaggtcgtga ttcgcgaccc catcttatct tccaaatcta catgccgcga
     8041 caaagcatct ttagtgtttg aataacccaa aacactcgca acgtctttgc ctacaaaata
     8101 tggttcatca tttactaaaa ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg
     8161 tgtgttcata tttctcattc ctttctttta tattcgtaaa caaatttaac aacttttttc
     8221 taaattcaat tgacagtatc tgagtaatac tctatactaa gagcataatt aaataagaca
     8281 taaaaacatt gattttaaag ctttcttggc ggtctgcatt tacatatcaa tagtgttttt
     8341 tgttgtcttt ttagttgtta aatctgttta caagatatag tatagctcat tactcagaat
     8401 tagtcaaatt atttctgcgt atttttctaa actttttttg taaacattta gaaaggttgt
     8461 tttatcgatg aatacctatg aaataataaa agacttggcg aagaggaaaa agatatctat
     8521 acggcagtta gaaattaatt ttggatattc taacggttat ctagcaaagt ggaaaaataa
     8581 tactcctaat tcagatgaat taagtcggat tgctgactat ttcgatgtct ctgtagacta
     8641 ccttctaggt agagaagaaa gagaaacccc taaacatgtg gatttatcag aagacgatac
     8701 tgttttttct tttgatggaa aagaaatatc taaggagaca atgcgtaaag cgattgcaat
     8761 tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg tttgtatgta tttgaaattg
     8821 aaagaaatgt tgagcgaata caatttgaaa gtagtttata tggagatgaa agagcctggt
     8881 ttctactatc caaaacccag aatcattttt ttgaatgaaa atctatacgg tgaaactgca
     8941 gaagcctttc atttatctca tgaacttgca catttcagcg cctctcattt tgaattttca
     9001 gttttgtacg atacttctac aacttttcac tcaaaatttg aaacagaagc agataaaata
     9061 gctattctaa ttttattaaa tatctatatt gagaacgaac tgactgataa atctcagttt
     9121 aatctcgaaa aatttatgga gtattattct attcagaata agctcagata cacttgttat
     9181 gctgtctgcc aatgctattt taagaaaaaa tattcttacg caaggcaata tgtatgaata
     9241 catcaagaat tattaaaaaa accgatttca gcaaaacatt aaaaaagccc gtgctgcaac
     9301 acgggctctt tcctcatata tgagcttcta caaaaaaaat catatcacag aaatgaggaa
     9361 gaaaaaatgg aaaaagagat tttaataaaa tctccaggaa aaaccttgat t
//
LOCUS       DACEMK010000097         9108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEMK010000097 DACEMK010000000
VERSION     DACEMK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.100"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            17..1204
                     /locus_tag="IW715_002220"
     CDS             17..1204
                     /locus_tag="IW715_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ1022815.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1185..2489
                     /locus_tag="IW715_002221"
     CDS             1185..2489
                     /locus_tag="IW715_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1022816.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2515..3528
                     /gene="citC"
                     /locus_tag="IW715_002222"
     CDS             2515..3528
                     /gene="citC"
                     /locus_tag="IW715_002222"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ1022817.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3532..3825
                     /gene="citD"
                     /locus_tag="IW715_002223"
     CDS             3532..3825
                     /gene="citD"
                     /locus_tag="IW715_002223"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ1022818.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3830..4735
                     /gene="citE"
                     /locus_tag="IW715_002224"
     CDS             3830..4735
                     /gene="citE"
                     /locus_tag="IW715_002224"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ1022819.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4728..6287
                     /gene="citF"
                     /locus_tag="IW715_002225"
     CDS             4728..6287
                     /gene="citF"
                     /locus_tag="IW715_002225"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ1022820.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6284..6841
                     /gene="citX"
                     /locus_tag="IW715_002226"
     CDS             6284..6841
                     /gene="citX"
                     /locus_tag="IW715_002226"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ1022821.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6834..7697
                     /gene="citG"
                     /locus_tag="IW715_002227"
     CDS             6834..7697
                     /gene="citG"
                     /locus_tag="IW715_002227"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ1022822.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7931..8340
                     /gene="tnpA"
                     /locus_tag="IW715_002228"
                     /pseudo
     CDS             7931..8340
                     /gene="tnpA"
                     /locus_tag="IW715_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8977..>9108)
                     /locus_tag="IW715_002229"
     CDS             complement(8977..>9108)
                     /locus_tag="IW715_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1022823.1"
                     /translation="RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3174 a         1420 c         1848 g         2666 t
ORIGIN      
        1 aaaaaggagt gaaggtatgc ttgatgaagt tttagaaatc cataaaaaaa atgtaggtgt
       61 tttacggatg gaaacaacag aagccgtgca agatgatcaa gatttagcaa aaatgtacac
      121 cccaggtgta gcagaactaa gtttaatgat tgagaaaaat catgctttgg caagagaatt
      181 aacaattagt ggtaaattag tggccgttat tacagacggg tcggcggtat taggtcttgg
      241 aaatgtagga aaccaagctg gattaccaat cgtcgaaggg aaagcgctgc tttataaaaa
      301 tttagcaggt gtcaatgcga ttccattagc catcgaacaa aaatcagtag acgagattgt
      361 acaaacaatt gtcaatttgc aaaatagttt tgcagggatc catttagaag atattgcagc
      421 accgaagtgt tttgagattg aagaaaaatt acaagaaaaa ctttctattc ctgtgtatca
      481 tgacgatcaa gaaggaacag cgatagttgt tttagctgga ttgatcaatg ctgctaagat
      541 ccaaaggaaa acattaactg aacttcgagt tttgatcaac ggcatgggag catctggggt
      601 tgcaacagct agactattaa tcgctgctgg tatcaaaaat ctaacattgg tggataaaca
      661 agggagatta aagaagcaag atagtacatt aaataaatat caaagagagt tgttggatca
      721 tatacatgat acaaataata atgcaagcgt tttggaagat gcaatcgttg gacaagatgt
      781 ttttatcggt ctttcaatag gaaatgtttt aacgcctgaa atgattggaa aaatgaatga
      841 taatccgatt atttttgctt tagccaatcc agtaccagag atcacacccg atttagcaaa
      901 agaatctggt gcaaagctaa tcgctacagg ttcttcgaaa tatcctaacc aagtaaacaa
      961 tattttagta tttcccggat tgttcaaggg actattacta tcgaaagcga ataaagtcga
     1021 tctttcatta caacttgaag tagccaaaca attagctgct aaggtcgcag agcctacggt
     1081 agaaaaattc atccctaatg tttttgatga aggagtcgca aacgtcgttt cacaggcagt
     1141 tttggattat ttacaaaaac taaaagaaaa cgaggataat atacatgctg aaaacattaa
     1201 atagaattaa aattggtttt attcctttat ctctttatat cattttggta ctcgcgtatc
     1261 ttgcaatgac gataagcaat atcgttccag aaaatgatat gttagtttct ttgggtatta
     1321 tgactgtttt tgcttattta ttagaagaaa ttggtaaaac tattcctatt ttaaaagatt
     1381 taggtggtaa agtattagtt gttacatttt tgcctgcatt tctagtctat aaacattggc
     1441 tacctcaaag tactgtaact gtggtttctg attttatgaa taataacaat ttcttgatgt
     1501 tctttatcac actactagtt gtcggcagta ttgcttcaat gaatcggcaa acattggtta
     1561 aagcaacttc acgtattatc attattttaa ttttatgcga tgtgattggt tctttgatag
     1621 gaacaggtgt gggaatactc cttgggctat ctgcttggaa tgcgtatttc tttatcgttg
     1681 cgccgattat ggctggtggt gttggggaag gagcgatgcc attatcgatt ggttatgcag
     1741 caatattatc tggtatggga caaaatgata tctttgggaa gattctaccg tgtgtgatgt
     1801 tgggaagttt agtcgcagtt ctactgtctg gtatgctcaa aaaaattgga gagaaatacc
     1861 cacaattaaa tggtcagggt aatttgatcg atggggatga tgaacattta gagaacttga
     1921 agggttcagc taaaccagtc aatattgaaa aaacattgtt agcaggcgtg gtagcaataa
     1981 cgatttattt tgttgctgtc tatgttgagc acttattaaa atcactcctt ggcttttcta
     2041 ttccttcacc tgtgatctta ttagtctctc ttatgggggc taaaatgctt ggttttatcc
     2101 ctaaaaatat cgaagaaggt ggaaattcgt tgtatggatt cactgttcgt gggatcacac
     2161 ctcccttact ttttggcgta ggggttgcaa aaacagattg gttagagctg atcgaagtat
     2221 ttaaaaatcc tgcaacgata gtaacaatca ttgtgacggt agtagctatt gtcgctacag
     2281 cttttatcgc cgcaaaattt ttaaaaatgt acccagttga tactgccatc gcagtttcat
     2341 gttgtagcgg acaaggcggt acaggtgctt tagctatctt agctgcgggt gatcgcatgg
     2401 aattaatgcc atttgcacaa gttgctgttc gactaggggg tgctatgacc gtaacgttcg
     2461 ctatcttttt aatgggtctg ctatcataag aacataaaga aaggacgtga ccatgtgttt
     2521 gatcaaacag atatccaacg attattttta aaaaataact atatcaaaga ccaatgggaa
     2581 aatcttttgc ttgattcagg aatacaaaag aagcaaatcg aagattttca acatttagat
     2641 caaacattgg gtatatacca taaagaaaag ttagtaggaa cagtatctta tcaaaacaac
     2701 gtgattaaat atatcgccgt gtctgaaaaa tataaagatg gtggacatac atttaatgta
     2761 ctagtctctg caataattca ggcaatgagt caacaagaga tttttcatta tattgttttc
     2821 acgaaaccgc gctatcaaaa aagttttaac tttttaggtt ttgaaacaat cgtatctacc
     2881 ccaaaaatag ctgtattaga aatgggagat agttctataa aacagtacct agagaggatt
     2941 cctaaagtga atggtgaaaa aaaagcagca attgtcatga atgcgaaccc atttacagaa
     3001 gggcattatt atttagtaaa acaagctgcc aataacaacg attgggtata tgtatttgta
     3061 ctcgaaaaag aacaaaatct tttttcgacg aaggaccgac tcaaattagt tcaacaagga
     3121 ttacaaaaac tgaaaaatgt cattgttcta tcaggtggcg agtatattat tagccctgca
     3181 acgtttccca cctatttttt acgagaagat gatgaaatag caaaggagca aatggtcgta
     3241 gatgctactt tattcaaaga aagaattgcc acttatttag atatctctgc tagatatgtg
     3301 ggagaagaac cactttcccc aatgactcgt tcatataacg acatactgga aacgatttta
     3361 ccccctgaaa taaaagttca tgtccttgca aggaagaaaa cggaacaaca ccaagttatt
     3421 tctgcttctc aagttcgtaa agcgtatctt gctgggcaat tggaaaaaat aaaatatatg
     3481 gttccagaaa caacctatca atacttaaaa aataaaaggg agcgttaagt aatggaaata
     3541 aataaatcag cgattgctgg aacattagag tcaagtgatg tccaaatcat gatttcaccg
     3601 aatacagaag gaatagaaat cgatttagac agtcaagtga ttgagcaatt tggaaaacaa
     3661 atcaaaaaag ttatctatga cacactagca gtttatcaga ttaaaaacgt aaaactcaag
     3721 gtagtagata aaggggcatt agattgcgtt attgttgcac gattaaaaac agcgatccat
     3781 cgttccttag gtatccaaca ccaagaaaca ccgtgggagg tttaattaga tgatgaaaga
     3841 acgtttgaga agaacaatga tgtttgttcc aggaaataat ccagcgatga tcaaagatgc
     3901 aggtatttat ggtgctgact cgattatgtt cgacctggaa gatgcagtgt ctatggcaga
     3961 aaaagacagc gcaagaatgc ttgtggctga ggcattgaaa acagtggatt atggtgacac
     4021 agaattaatt gttcgaataa atgacctgca ctcagaattt ggacgtgcag acatttttgc
     4081 aatggttaaa gccggcgcgc atgtattacg tttacctaaa acagaaacct ctgaagatat
     4141 aacagaagta gaaaaagtga ttatagaagc agaaaactat cataatgttc cgatagggcg
     4201 aacattgatg atggcagcca ttgaaggtgc ttctggtgta ttgaacgcac ctgcgatcgc
     4261 caaagcatct gatcgattga ttggaatcgc cattggggca gaagactatg tgacaaatat
     4321 gaaaaccaaa cgatatccag atggaaacga attgtttttt gctcgcagta tgatcgttca
     4381 tgctgcacgt gcagcaggca tttccgcatt tgacactgtt tactctgatg tggatgatat
     4441 ggaaggattt ttaaacgaag tacgtttaat ccatcaatta ggttttgatg gaaaatcttg
     4501 tatcaatcca cggcaaattc cagtagtcaa tactgtttat cagccgtcaa tcaaagaaat
     4561 caaacgatca ctagacatca tctcggcaat cgaagaagca aaagaaaaag gttctggcgt
     4621 aatttcttta aatggaaaaa tgatcgataa accaattgta gaacgtgcac aacggacatt
     4681 gatgttagca aaagcatcac gattgattga tgaggaggga aatatccatg cataataaac
     4741 aaagacgtag cattccatca agtgttttaa caaagtataa aaaagaattc caaagtgttt
     4801 cattatcgcc acaaacgatt caacgttgtg caccaaaagt aacgactgta actaatcaga
     4861 caaataaatt gatgcactcg atcaaagaag ctatagaaaa atcgggctta aaagatggaa
     4921 tgacgatttc atttcatcat catttgagag aaggtgattt tgtttttaac caagtgatgg
     4981 ccgtgatccg tgaaatgggc atcaaagaat tgaccttagc accaagttct ttaacgaaca
     5041 ctatgaatga gatggttatt gaatgcatca aggacggtac agtaactcat atcacaagca
     5101 gtgggatgcg cggcagctta ggtgaattta tttctcatgg aggaatggac aatcctgtga
     5161 ttttacgttc tcatggtaat cgtccacgtg caattgaaag tggtgaattg aaaatcgatg
     5221 ttgcatttct aggagtacca acatctgatg attatggaaa tgctaatgcg actgttggaa
     5281 aatccgtttt tggttcatta gggtatgcaa aagtagatgc acaatatgca gataaagtca
     5341 ttcttctaac tgatcattta gttccttacc ctaatacgcc cattagtatt ccgcaaacac
     5401 aagtcgacta tgtcgtggaa gtagaagcaa ttggtgatcc gaataaaatc ggtgctggtg
     5461 ctacacggtt tactaaaaac ccaaaagaat taaaaattgc tgaaatggtc aaagatgtca
     5521 ttgttcactc accttatttc aaagatggct tttcttttca aacaggaacg ggtggtgcag
     5581 ctttagcagt aacgcgttac ttagctagtg aaatggaaga aaaacagatc aaagcttctt
     5641 ttgcattagg tggcatcact aaaccaacgg ttgatttatt ggaaaaaggt cttgttgaaa
     5701 aagtgttaga tgtccaagac tttgacaaag gagcagctga aggaatggcg cggcttgaga
     5761 accaacagga gatcgatgct tccttttatg ctgatccaag taataaagga gcggttgtta
     5821 accaattaga tgtcgctatt ttatctgcat tagaaattga cacaaacttc aatgtaaatg
     5881 ttatgactgg ttcagatggt gtgctaagag gtgcgattgg tggacatcaa gatgcagcaa
     5941 cggctaaact tactatcatc tcagcgccgt tagtacgtgg ccgaatacct acagtagtaa
     6001 atgatgttac tacattgatc acaccaggga aaagtatcga tgtattagtc acagaagtag
     6061 gggttgctat caatccccaa cgtaaagatc taatagctat ttttgaaaga attccacaaa
     6121 ttcctgtttt tacaatagaa gaattacagc aaaaagcaga aaaaatagta ggcatcccgg
     6181 aacctttgca attcacagat cggatagttg cctatgtaga ataccgagat ggttcaattt
     6241 tagatgtcgt tcgtcaggtg aaagaatttg aagaagagag atcatgatgt ttgatactgg
     6301 agaaattcaa gaaatcgagg atattttgat ccataaagaa caacgtgcta acttgcaaag
     6361 caacctgctt tccaagtatt caaatagttc attgatggtc tttacattga atattcctgg
     6421 gccgatcaaa aataatccaa tgattgagca ggtatttcgt cagggacagc agtcaattca
     6481 gcaaatgttt ttaaaaaaaa aggtggaaat gacgtacaca aaagaaatca atttgccaag
     6541 tggattggac tggtacgtaa tagtaacaga agcacctttg aatctcaaaa aatggttggt
     6601 cacgttagaa gagcaagacg cattaggtag actttttgat ttagatgtct tgttcaaaaa
     6661 aggagagcag ataacaattg tcagtcgcca agctcttaat catggggaac gtccttgttt
     6721 tatttgtggg aaatcagcaa aggtatgtgc acgtaatcgt acacatactg tgaaagaaat
     6781 gcaaaagaaa atcgaacaaa tagtagccga ttccttaaaa ggagcattga aacatgcatg
     6841 ataatattca aaaaatagca caagaagcac aattagcgat gctttatgaa gttacatgta
     6901 tcaataaacc aggattggtt gatccagtag accccgggtc tcatcaagat atggacattt
     6961 ttacttttct acaaagtagt gtagttttga ctccctattt tgaagaattt atccaaacag
     7021 gattggaccg acaacatcaa ccaattgaag aaacctttat ggcgattcga cagattggtt
     7081 tagaggctga aactgcgatg ttctcagtga caaatggtat aaatacacat aaaggtgcta
     7141 tcttttcatt ggggattttt cttgctattt gtggccggtt aactatttgg aaagttcctt
     7201 gtaaaaaaag tttctttcaa aaagaaattc aaaaaatgac gaaaaatcta cttaacgatt
     7261 ttgggaagat tgatcaaaca aaaccaaaag catggacttg gggagaatat ctatttgtta
     7321 accatggatt gacagggata cgaggggaag ccaaaagagg ttatccttgt gtttttgacc
     7381 gaggattgcc ttattatcaa gcatatcaag ggtcgcaaca agataagatg atagacactt
     7441 tactttttct atcaatggaa gtagaagata ccaatttgat caaacgaagt aataatagac
     7501 atgtttttga agagtatcaa ttacttattc gtccttattt tgaattaggg ggtgtaaaaa
     7561 ccatacaagg taaaaaatat ttggattacc taaatcagca atttaaaaag aaaaattgga
     7621 gtattggtgg aagtgcagac ttattgatcc ttacaatctt tttagataaa atcatggaca
     7681 aaggatggtt atgttgatac tataatacat tattttgtat tagtaagcta ataactaaaa
     7741 aatggttcaa tacaaatatt ttataaaaaa tggtagtgcc tatcctttca gattgtagac
     7801 aaagaaaaat ttctttgtct acaatttttt tgttaatgac ttcagtcagt ttcgtgcgca
     7861 aagcgtgcga aacaagaaaa ccatgcgttt ctacacgtgg aagaactagc atatcgaaag
     7921 gagttttttc atgtcaaatg acgataaaag tttagcccat actcgttgga attttaagta
     7981 tcatattgta tttacaccaa aatatcgacg aaaagtaatc tataggcaat tacgaaaaga
     8041 tattggaaaa atacttagga agttgtgcaa aataaaaaac gtggaaatta tagaagcaca
     8101 tgcaacgcca gatcatattc acatgcttgt tcaagtacca tcaaaaataa gtatatccag
     8161 ttttatagtt atctgaaaga tcgtagtgct gtgctaatcc acgagcagca cgtaaatcac
     8221 aaatatagat acggaaataa atcattttgg tcggaggaat attatgtgag tacagtcgaa
     8281 ttgaatcaaa agacaattga aaaatacatc cgtgaacaag aagctgatga tcgcatttga
     8341 gatagtataa gtaaaagaga gtagatgttg caataacaaa agggtaaaaa tggactacct
     8401 ccagtaaaat accgaaaaat agtttaactt ttggagataa gtccatttaa gggtctatag
     8461 aatgacaatt tttttagggg attttactat tattacttca agacccgctt ggattcgcaa
     8521 agtgagtgaa ctcttaaaac tacctgatag taggttgttt taagagttat ctgaaagtaa
     8581 aaattgcatt tatatttttg ataaatacgt aaatttatat atacgtattt atcaaagttt
     8641 taggccaaag gaatattgtg taaatacact aggattgaat gaatagacca gaagaaattg
     8701 cgtccaagaa caaaagaagc atgatagtgc tctatataaa ttgggcattc aagaatctga
     8761 aatttaaagg tagaatttta gttagacttt tagattttaa acacaaaaat aaccatacaa
     8821 gcaacaagaa gcaaagtgct atattataaa ttgtccgtcc gtttacttac ataagatcat
     8881 tggggctatt tttaaaagag aataatagat aataaatgaa aaaaaagtca agtccacaat
     8941 cctgtgtaaa atactataca atgctctcac tattttttac ttggcaaatt taatatattt
     9001 tcttgatgaa gaaagccact catcgtgttg atcaataagg actgctccaa ttaaacgatt
     9061 ggctgaaacg cagtttggaa agatacgaat aattttttct cttctgcg
//
LOCUS       DACEMK010000098         9087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEMK010000098 DACEMK010000000
VERSION     DACEMK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.101"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            806..1006
                     /locus_tag="IW715_002230"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IW715_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IW715_002231"
     CDS             1493..1672
                     /locus_tag="IW715_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022824.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IW715_002232"
     CDS             complement(1718..2323)
                     /locus_tag="IW715_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1022825.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IW715_002233"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IW715_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IW715_002234"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IW715_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IW715_002235"
     CDS             3358..4329
                     /locus_tag="IW715_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1022826.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IW715_002236"
     CDS             4469..5782
                     /locus_tag="IW715_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1022827.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IW715_002237"
     CDS             5825..6691
                     /locus_tag="IW715_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1022828.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IW715_002238"
     CDS             6688..7536
                     /locus_tag="IW715_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1022829.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IW715_002239"
     CDS             7550..9022
                     /locus_tag="IW715_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1022830.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
BASE COUNT         2894 a         1513 c         1853 g         2827 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgcca
//
LOCUS       DACEMK010000099         8817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEMK010000099 DACEMK010000000
VERSION     DACEMK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.102"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(310..807)
                     /locus_tag="IW715_002240"
     CDS             complement(310..807)
                     /locus_tag="IW715_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1022831.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IW715_002241"
     CDS             complement(847..1656)
                     /locus_tag="IW715_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1022832.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IW715_002242"
     CDS             1990..2847
                     /locus_tag="IW715_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1022833.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IW715_002243"
     CDS             2840..3742
                     /locus_tag="IW715_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1022834.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IW715_002244"
     CDS             3775..4125
                     /locus_tag="IW715_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1022835.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IW715_002245"
     CDS             4159..5097
                     /locus_tag="IW715_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1022836.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IW715_002246"
     CDS             5175..6068
                     /locus_tag="IW715_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1022837.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IW715_002247"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IW715_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1022838.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IW715_002248"
     CDS             6683..6871
                     /locus_tag="IW715_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1022839.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IW715_002249"
     CDS             6884..7786
                     /locus_tag="IW715_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1022840.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IW715_002250"
     CDS             7818..8060
                     /locus_tag="IW715_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1022841.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="IW715_002251"
     CDS             complement(8316..8687)
                     /locus_tag="IW715_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022842.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3073 a         1522 c         1705 g         2517 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
     8761 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataat
//
LOCUS       DACEMK010000100         8680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEMK010000100 DACEMK010000000
VERSION     DACEMK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.103"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..245
                     /locus_tag="IW715_002252"
     CDS             <1..245
                     /locus_tag="IW715_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022843.1"
                     /translation="IYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYN
                     PLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(371..1048)
                     /gene="nth"
                     /locus_tag="IW715_002253"
     CDS             complement(371..1048)
                     /gene="nth"
                     /locus_tag="IW715_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1022844.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1055..1753)
                     /locus_tag="IW715_002254"
     CDS             complement(1055..1753)
                     /locus_tag="IW715_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1022845.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2021..3205
                     /locus_tag="IW715_002255"
     CDS             2021..3205
                     /locus_tag="IW715_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1022846.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3211..3654)
                     /locus_tag="IW715_002256"
     CDS             complement(3211..3654)
                     /locus_tag="IW715_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1022847.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3880..4167)
                     /locus_tag="IW715_002257"
     CDS             complement(3880..4167)
                     /locus_tag="IW715_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022848.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4338..5690)
                     /locus_tag="IW715_002258"
     CDS             complement(4338..5690)
                     /locus_tag="IW715_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1022849.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5963..6409)
                     /locus_tag="IW715_002259"
     CDS             complement(5963..6409)
                     /locus_tag="IW715_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1022850.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6645..7571)
                     /gene="cysK"
                     /locus_tag="IW715_002260"
     CDS             complement(6645..7571)
                     /gene="cysK"
                     /locus_tag="IW715_002260"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1022851.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7711..8598)
                     /locus_tag="IW715_002261"
     CDS             complement(7711..8598)
                     /locus_tag="IW715_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1022852.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2366 a         1774 c         1434 g         3106 t
ORIGIN      
        1 gaatctatat ttttctgatg agcctgaaac cgcccaaatc cattagttta tgtttagtta
       61 taaatggtat ttttttatta tcacttatca tttttattac acctttagat agatacgtca
      121 cttatttata taatcctttg gaatactgga cttattttat aggtatattg gaaggcatta
      181 ttttctacat cgggtatttt cccaataaaa ataacaactt ttatttttat cgcaataaat
      241 tgtaataagt taccatccta gctactgagt atgtacattt actttttcat gcaaaaaaga
      301 taaacgaaac agcttttctc cataataaca agataattca tacatttatt tgcaattaaa
      361 gaaaccacgt ctacaggcgt ggtttctttt ttagcgcctt ttcttttaga cgcatccgat
      421 ttttcccatc ttgacatatt gatagcaaag gacatacctc gcacttggga tttcttgccg
      481 tgcaatgata tctaccaaaa aaaatcagtg tatgatgtgt ctttacccat aattcttgtg
      541 gtactttccg catcagtgtt tcttctactt ccatgaccgt agcatctagt ttgcagatcc
      601 tcaggcgttt tgagacacgt tccacgtggg tatctactgc gattgctggt atcccgaatg
      661 catcccccaa tacgacattt gctgtctttc tgcctacacc aggaagagac atcaattctt
      721 ctcttgacgt tggcacttgc ccatcgaaac gttcaatcag ctgctgtgca cacgctttga
      781 tgttttttgc tttatttcga tacaaaccaa tcgtttttat tttaagaatg atttcatcga
      841 ttgaagcttc tgctaacgca tcgggtgtcg gaaaagaggc aaacaaatct ggcgttgctt
      901 tattcactga tacatctgtc gcctgggcac ttaagatcac tgcaatcaat aactcaaatg
      961 gattattgtg cttgagttct ccatgtgctt cgggaaacat tccatacatc gtttctagag
     1021 cttccattgt tttttgtttg cttaacatat tccgctactc ctccaaccaa tttttcagtg
     1081 agactttcgg caattcttct tgttgagctg actgttctac ttctctttga agaagctgct
     1141 gttttcttcg tttttggtct tcttccactt gttgttttgt acgtagattt tttctttccc
     1201 acgatagcaa aatgcggtca acgtaattaa aactataagc ctgatttaat acggcttccc
     1261 gcagtgctag cttcaaaatt tccggctgat aatggtcttc ttctagccat tgtccgatcc
     1321 gctggaactc gatcgaagaa agcgggcgtc caaattcttg ttcaaataat tgatacactg
     1381 actggatttc tttttcttgt gttttttctt cgtatttttt ttcttgtgtt tttaaatact
     1441 cacctaatag atcatagatg gggtaaagat tatatcgatc tgctttttta ccgttgacaa
     1501 tcgtcgtttc tatcttgata aatccatttg ttacgagacg atccagtatt tgaaaaatct
     1561 gatcttgttt gattcccata tccatagcaa tcatctgcaa atcagggaaa gtatctcctt
     1621 caagctgtgc catatgcaac tgtagaacaa ataaaaattc ttcagattgt agaccgatcc
     1681 tatgataatt tttaagtaaa agattagata tgactgtttg tccagcgctt aagtaatttt
     1741 ctaaattcaa catgatttct cctccgctca atcacagtat acgtaaaaaa aacatgggaa
     1801 gcaagaacag gtttcttttc tatttacttc actgatcggt cagatttaca atgtcgtcta
     1861 cttgattttt gtttgttcat gtttttgatg cgtatcttca cttttcggaa taaataaatt
     1921 aagtagattt cgctgttcct tagaatgttg acaaggaaac aaaattacta tacactattt
     1981 ttgtttccaa gttaacatta ttgcataaag gagtttttac atgtacagtt tcacaaataa
     2041 acattctaaa gtttttggat acgtctctgt cttattcact ggattaggat ttactatcgt
     2101 cagtcctgta cttccgtttt taacgctgcc ttattctcac tccatacatc agcaagcttt
     2161 ctatatcact ttgctcatgt ctgtttatgc tctagctgcc ttcttatctg cccccattct
     2221 cggtagctta agtgatcatt ttgggcgtca tccgatcctt attatcagtt tattaggatc
     2281 aagtatcgga tatttgcttt ttggtttagg aaattcgata tggatgttgt tccttggtcg
     2341 tatcattgaa ggattgacgg caggagaaat ctccacctta tatgcttact ttgccgacat
     2401 cacggaacct aatgaaagaa ccaaagtttt tggatggatg ggtgctttag tcggtatagg
     2461 aacaacgctg ggtcctatta tagggggtct attagctgag ttggggaatt ctgttcctat
     2521 atttattgga gcactcttta ctttcttaaa tgccgtttat ggctacacat ttatgctaga
     2581 aagccttcca ataaaaaaac gttcagtaga tttaagtttc tcccatgtaa gaccttttca
     2641 ccaattacaa caattattca agatttcatc cgttattcct ttattgacag caggttttgc
     2701 tgtttggttg gcagctggct ctcttcaatc tatcttttct caattttcga ttgacgcctt
     2761 tcagtggaag gctgggttgg ttggtttaag cttctctttg attggaatct tggatactgt
     2821 ctcgcaattg ttcattatgc ctcgattgct caaaaaattt tcagaacagc agatcacaag
     2881 aatagggatg ttcagtgaaa tcctcgccta tctattcatc acattatctg gaattcttct
     2941 actgccgatt ttgtttttat ttgggattat ctgttatggt ttcggtgatt ctatttttac
     3001 tcctgtattc aatggccaac tatccaattc tgtctcagat aaccagcaag gtcttgtaat
     3061 gggcggtacc caaagtatac aatctctatc gcgcgtcatc ggcccgctaa tcgcaggaca
     3121 gctttatgct gcagcaccat ttcttccgac ggtattcgga tttttgctta tgatttttgc
     3181 ctattatcac tacaataaaa aatagtagct tcatttgcta cttcgtataa attctctgta
     3241 tcgaccgata aaacgaaata ctgtgtcaaa ttcttctttt gacatatttt caaatacagt
     3301 agcatctcgc tgtacccatt tttgatggag ttgctcgtgt ctattcctta ttttttgtcc
     3361 gattggcgtc aattgaaaat agatttcttt tttattctct tcgctttgat aagtgtttat
     3421 gatccctcta gctatcagtt tttttgttag cttactgatg gctcctctag tcatgtaact
     3481 agcattcgct aattttgtca cgttaggatc agggattttc ccgatatgtt caatcacttc
     3541 tagttcattt agagtaaatc ctgcaaattc tttttccatt tctggtttat tacgatgaat
     3601 aatctcatta gctaaatcca gatattcttc tattagcagt tgtcttttat ccatttctac
     3661 accttctttt tctttcatta tattgttttt ttgtttccta ttcaacaaag ataaaaatat
     3721 tttgtaggaa aacccaatct gaaaccaaga aaaaaagggg ttgacaaagc ttgtcataac
     3781 ccctttttct gattaaaaga tagtaaagag acagtcatcg gtaattgtga tgattttttg
     3841 tcatcactta aggatgagcg gccttttcaa aaactgtctt taaagatcat catcagtttc
     3901 tagcacatat ggctgacctt catatgtgta ctctgtattc aatgtgccgt cattttcatc
     3961 tgagtagatg cgtctactga aaggaaaacg atgagaaaca ttatctttgg cgtcatttgc
     4021 tggcatcgtg taatattcct cttgcgtttc attcaactga gagagtactt gatctgccaa
     4081 ctcatcaata ttgatcgtta atttcttcga agcactttta taatcttcta aaaatgcatc
     4141 ttgttggaca aaatattctt tattcatctt ctttctcacc tttcttttca aaatcaaata
     4201 aagctgcttg ttcttttatg taacagcttt atctttgctg cttattgtga caaaaaattc
     4261 gaagaaagac aactaattgt acttttcaca aaaaaagaca gagggtcgtg acaaaattgt
     4321 gtcacaaccc tttttcttta ttgtgcagtt tcgatttcgc ctagcgctgc ttgtgcaagt
     4381 aggttcaagt aattccatgg gcggtcaaaa tgtggttgga agaagaaatc tgaaagtgcg
     4441 agatcttcta cagtcatttt attttgaaca gctaaagaca tggtatttgc tgattgtgta
     4501 atgtcgtatt tggacatgaa ctgagcacct acgatacgat tcgttccttt ttcatagact
     4561 agctccatca ttactttttc agtagttggc atgaactctg gacgataatt atcttcaaat
     4621 acagtcgctt ggacttctag atcgttcaaa gttgcgcttt ctttggtcac acctgtcgaa
     4681 ccgatcgtcc agccaaatag gtaaagtcct gaagttcctt gagtaccacg atacgccatt
     4741 ttatgatctg tcaagttatt tcccacaagc aaaccttgac gaacggcatt tgttgccaaa
     4801 ggaatatagt tttgcgtgcc acttggattg tagttgacta ctgcagagtc tccagctgca
     4861 aaaatgtctg gattacttgt ctgcatgtat tcattcactt tgattgcccc gtttggcagt
     4921 gtatctactt ttccttcgat cagttttgta tttggacgga agcccacaca taaaatgacc
     4981 atatctgtat caaatgtatc tgttggtgta gctacttgtt tcacttttcc ttcttcatct
     5041 gtgataaatt cagtaacatt ttcgcctagc gcaagagtaa caccgcggtc tgtcaattct
     5101 ttttctaaga tatctgtgaa tggtttatct agatatttat tcaagatgcg atctaatcca
     5161 tcaatcaatg tcacttgttt tccagattcg acaaaagctt ctactaattc aataccgata
     5221 tatccaccac caacaattac tacacgtttt gcttctttcg cttgtgcaat aatttcattt
     5281 gcttgattgt agtttttgca taggagtaca tttttcgcat cgattccact aatcggtggg
     5341 ataataggcc atgaaccagt tgtcatcact aatttatcat aagaaaccgt ttctgtttct
     5401 cctgttttca gatcttttgc cgttactgtt ttattttctg tatcgacgtc tgttacatcg
     5461 tgttccattt taactgttgc tcctaatgaa gataattctt caggatttga gtagaacaag
     5521 cctgctgggt cctttactac tccgccgaca taaagtgcga taccgcaaga taagaaagaa
     5581 atattgtcat ttctttcata tactgtcact tctgcttctg gatgattttt taaaatactt
     5641 tttacagctg cagtacctgc atgagtacag ccaattacta ctactttcat ttatggtccc
     5701 tccgattaac attttgtcct tcgacaaaac tactataaca gaaataaaac ggatacaaca
     5761 gtttaatgct tgtgaattca caaaggtttg tttgtagttt cattcctttt caagcataag
     5821 aacccaatat ctacaagatt cttattttaa taatattcac ttttatatct aagattgtga
     5881 ataaaagaca aaaaaataaa aaagctagcg ttttttttac acagctagct ttttttaggt
     5941 ataacacttg tagaaactgt ttttacattt gcgccttttt tgctgactct ttacaatccg
     6001 gacagagtcc atacaattcc aaccgatgtt cattaatctc aaaatcagtc agtctgcttg
     6061 cagccatttc tatatcttca agccctgggt aatgaaaatc aacaatctta ccgcagtttt
     6121 cacaaattac atgataatga cgctttgaac tgaaatcaaa tcggctagaa gcatcgccgt
     6181 acttcatttc ttgaacaaat ccaatcgaag taaataagcg aaggttattg taaacagtcg
     6241 caacactcat atttggaaac cgatgttcaa gtgctttata aatctcatca gccgttggat
     6301 gggaatgatg ttcaatcaaa tattctaaaa ccgcgtaccg ttgaggtgtg attcgaatgt
     6361 tctctttttt taacttttcg ataacctctt cgactaactt attttccatg taaacacccc
     6421 acgttgttat tctcgtctta tcatactatt tttacatttt aaaatccact taacatgaaa
     6481 agaaaattca attttattgt attttaatga aaaaagacgt aaaacagtcg cgttcactat
     6541 tctacgtctc tattcaaata atttttctca tcataaattt ttgtgtgttc ttttcatctt
     6601 taaaagtttt ttacataaac ctatctggag aaaaaccaag tgccttatat ctcttggtaa
     6661 agggctgtag ataaataacg ttcaccattg tcgggaacca cagctaatac cttcttgcct
     6721 tttcctaatt cttttgcgac ttttagcgcg gcagcaattg cagcgccaga tgagatgcct
     6781 actaaaatcc cttcttttcg cccaacttct ctagctgttt ccatcgcttc gtctccacta
     6841 atagaaagga ctttatcata cacagaagta tccagtgttt taggaacaaa acctgtaccg
     6901 attccttgga ttttgtgagg tcccgcttct ttcccttcta aaatagcaga ttctgctggt
     6961 tctaccccga ttaattcgat tttcggatac acacgtttta attctcggcc agcgccagtg
     7021 atcgttcctc ctgtaccgat acctgcaaca aacgcgtcca agccatttac tccaaatgct
     7081 tgatgtatct caggaccagt cgtcttctca tgcacaagag gatttgcttc attttcaaat
     7141 tgtaatggta aaaagtagcc gttttctttt gccaaacgtt ctgcttctct gattgatcca
     7201 gaaataccat ccgcaccagg agtcaaaatc aactctgcgc cgtacccttt catcaataaa
     7261 cgtctttcga tactcatcgt ctcaggcatc acgatgatta ctttatatcc ttttgcaact
     7321 cctaccatcg acaatccgat tcctgtattt ccagaagtag gttcaatgat agtatcacct
     7381 ggtttcagta gaccatcgtg ttctgctttt tctatcatac ttaaagcaat acgatctttt
     7441 acacttcctc ctggattaaa aaactcaagc tttacaaaaa catctgctga atcttctggt
     7501 acgattttat ttaatttgac gattggcgtt ttgccaatca attcggtgac tgagttatag
     7561 atagctgtca tcttcaattc ctccttgtct ttgctcttct tacttatcat aatacaatct
     7621 gttcatttat ggcgagtgat tcgtctttga ctagcaaaaa aaagacaaag atataccata
     7681 tatggtaatt tctttgcctt tttatctaaa tcaaacgttt agttcttgac caactaataa
     7741 agaataactg gacatagaca tgccattttt cgccattaga tcatcaacag acatgccaaa
     7801 tttttgtgca atatcccata gcgtatcacc tgacacgact gtatatgtct gaccattatt
     7861 tgtatttaca tctgttgttg aagatgatga cgttgaagtc gtcgtagtag tcgttgttgt
     7921 tgtacttgtg attgttgtag ccgaacctgt tgatggcgta tcaaaacggg ttaaattata
     7981 agtattaatc aatgagttca atttggcccc ataatttgga tcggtagcat aacgacctgt
     8041 caagtacgct gttgcatcat aaaaacttgt tgtattgctt ttccacacgc cagcataatg
     8101 ataatcgccc gtggaaaaac tggttgaacg aagaacattc gcatggtctt ggaaggattc
     8161 ccaataagaa gaatattttc ggaatggctc attcatcgtt acccattgtc cgtttacata
     8221 ttctttggtt gccatataaa cgacctgtcc gttatagctt cctttcacac cgaatagatt
     8281 ataatttggc gcactagcta atccggaagt gccatatgca ctttccaaca acgcttgagc
     8341 gatcatgact gaagcataca agtcattact tgcagcaact tctgacgcag accagccgat
     8401 ttgatcgata aacgcttgag gatcagcgac tgtttgagat ccttgttcat ctgcatgaac
     8461 atgttgcggt accgctgtaa ccatcatcgg cccaactaaa agactcgttc caattaaagg
     8521 agcaatcgct tttttattga tcaccatttt tcttttaggt ctatggtgcc gggcactgcg
     8581 ggtcttcagc tcttgcattc aaattcctcc tcaaaataaa aaaacttaat aaaaagttct
     8641 cttcttaaaa caaacttaat taattaaatt atacttttaa
//
LOCUS       DACEMK010000101         8576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEMK010000101 DACEMK010000000
VERSION     DACEMK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.104"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(312..842)
                     /locus_tag="IW715_002262"
     CDS             complement(312..842)
                     /locus_tag="IW715_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1022853.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1024..1680)
                     /locus_tag="IW715_002263"
     CDS             complement(1024..1680)
                     /locus_tag="IW715_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1022854.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="IW715_002264"
     CDS             complement(1807..2280)
                     /gene="dfrF"
                     /locus_tag="IW715_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1022855.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2699..2917)
                     /locus_tag="IW715_002265"
                     /pseudo
     CDS             complement(2699..2917)
                     /locus_tag="IW715_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2948..4162)
                     /locus_tag="IW715_002266"
     CDS             complement(2948..4162)
                     /locus_tag="IW715_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1022856.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4503..4745)
                     /locus_tag="IW715_002267"
     CDS             complement(4503..4745)
                     /locus_tag="IW715_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1022857.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4777..5334)
                     /locus_tag="IW715_002268"
     CDS             complement(4777..5334)
                     /locus_tag="IW715_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1022858.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5347..5535)
                     /locus_tag="IW715_002269"
     CDS             complement(5347..5535)
                     /locus_tag="IW715_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1022859.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="IW715_002270"
     CDS             complement(5548..6114)
                     /gene="amaP"
                     /locus_tag="IW715_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1022860.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            7344..7817
                     /locus_tag="IW715_002271"
     CDS             7344..7817
                     /locus_tag="IW715_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1022861.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7826..8053
                     /locus_tag="IW715_002272"
     CDS             7826..8053
                     /locus_tag="IW715_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022862.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2607 a         1684 c         1274 g         3011 t
ORIGIN      
        1 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
       61 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      121 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
      181 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
      241 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
      301 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
      361 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
      421 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
      481 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
      541 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactct
      601 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
      661 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
      721 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
      781 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
      841 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
      901 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
      961 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1021 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1081 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1141 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     1201 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     1261 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     1321 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     1381 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     1441 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     1501 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     1561 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     1621 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     1681 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     1741 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     1801 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     1861 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     1921 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     1981 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2041 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2101 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     2161 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     2221 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     2281 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     2341 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     2401 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     2461 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     2521 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     2581 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     2641 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     2701 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     2761 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     2821 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     2881 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     2941 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3001 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3061 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3121 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     3181 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     3241 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     3301 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     3361 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     3421 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     3481 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     3541 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     3601 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     3661 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     3721 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     3781 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     3841 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     3901 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     3961 aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4021 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4081 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4141 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     4201 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     4261 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     4321 aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt tatttttaaa
     4381 gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa agaaccgcag
     4441 cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt tcttcaattt
     4501 gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc accgatcaat
     4561 gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca
     4621 attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga tgatccttta
     4681 ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc ccaaataaat
     4741 cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg gttcgttatt
     4801 ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg tttggcgtga
     4861 agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg tttcttgatc
     4921 tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt cgataacatc
     4981 taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt tgtaaatatc
     5041 ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt gtttcttacc
     5101 tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt cagccatatt
     5161 tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg cataaccgat
     5221 gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc ggttttctcc
     5281 tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt ccatctttgt
     5341 ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat aaaaaccgat
     5401 tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc caatcgttaa
     5461 ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct tgtatgctga
     5521 aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt agtctcttgt
     5581 atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt cttttgttcc
     5641 gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt tccctactaa
     5701 tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt tcgttgaacg
     5761 tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc
     5821 ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa cgaaagttcc
     5881 tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa cggcaaatac
     5941 tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa agaagcgaaa
     6001 tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg ctagaaatac
     6061 tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat tcattttcca
     6121 cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca gtatagatag
     6181 cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg atcataacgg
     6241 cgttttattg ccagtctgat actgaaataa caattgctta cttattctct atttactttt
     6301 gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag attatgctca
     6361 gttattatta caagattcta tttatttatt aaattttttc ggagaattaa aaaatacaat
     6421 aaataacttt agttacttta atgcgttctt actttatttt tatattatca tttttcttat
     6481 tcacttcttt ggtttatact attcttataa ataaaaacga ggtatgtcct atgatgaaaa
     6541 gattgtacta ttcattgatc ataacgattg gctatttgat tgtatcaaat ctaggaaata
     6601 tggtatttgg tatcagtaaa gaattcagtt ggactactac attatgggaa tctctattct
     6661 ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc
     6721 tgttggcaca tgttttagga tttatggtac tttttattcg gcaatccatt ttatttttct
     6781 acttcttctt aatacctcta acacttttga tttataaact aatccaatgc atctacttgc
     6841 gcttattaag ctatttcagc tagatagtac caccccatgc gtcaatgctt gattttcctt
     6901 ttgtataaca ttgtataagc aataaaggac aaataagtgg tgattacacc aaaccattga
     6961 ataagaaaaa ttctgatacg taaataaaca tgaacttgaa aaaattaatg aatcggttag
     7021 ctactacgta gttgtctttt ttctcataca gttctgttaa cttacacttc tcttcgctag
     7081 ataataaaaa atcctccaac tgtattgtcc aatataatcc caggcatcta aactagcatt
     7141 atagaagaga cacacataga atctaaaata actggtttta aatgtttctt acaatcaaaa
     7201 aaagctgtta aatgcactcc acaccttcat actctatcat tttttataaa cgatatattt
     7261 ttattgtttt tcgataaaaa atagttgttt tacaataatt attctgctat attttagcta
     7321 tcaaagataa aagagggata aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt
     7381 taggtataag tgccgtattt attttgttcg gcgtactttt ttatactcag atcgctacaa
     7441 gcgaaaaagg attcaatttt gattggcaga ccacattatt tgtttctgtc atttactttg
     7501 cccttctcct agggatcgtt attgcttatt tcttattgaa actattgaaa aaaatcgaag
     7561 ataaccaagc tttttccact caaagcttaa acatactaaa aaaaatcgaa cgaaccattt
     7621 tgtttattgg tttcgccttt tttggcttac ttcctaaatt tatgaaagta gcggatttag
     7681 acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt attcattcct tttgcagtat
     7741 acacattaac aagcgtgctg gaaaaactgc ttgaacatgc gattttactt aagacagatc
     7801 atgatttgac agtgtgaggt gcgacatggc aattatcgtt aatttagatg tagtattagc
     7861 tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg ggcattacga tggcaaacat
     7921 ctctattcta aaaaatggaa aagcaaaagc catccgtttt tccactctcg aaaaaatctg
     7981 ccaagttttg gagtgtcaac caggtgattt gttgacctat aaaaatgact cgaatactat
     8041 accagaagaa taaaataaag tagaacaaac agtaaataaa aaataatctg aatgatagat
     8101 gtgtgagact tttagaagtt atttacgtct attattcaga ttttttattt cgattgaata
     8161 ccgttttatc tataaataag atatgtactg actcatacaa agggggcctt ttcttctcca
     8221 tgtactatca cttttcctga tacaactggt ttcctgttac gttgtagttg gatctgcttt
     8281 tagtataata ctttattcat tccaccagac tgatagcagt ttgagcagtc taaataatca
     8341 tgtagtatta caggccattc tgtttcatac cttgaagtgc ttaaaacttc agctaatggc
     8401 ccattgtgtt aactcttact ccattctttt ttagtaagct cccatatata ctgtaaagcc
     8461 ttctatatat tatgtcatgt tgctttttaa catttactat gtagttttga taaaatgatt
     8521 tgaataacag tgatggacaa ttctttttta tttttaacat tattttgata ataatg
//
LOCUS       DACEMK010000102         8574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEMK010000102 DACEMK010000000
VERSION     DACEMK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.105"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(151..1104)
                     /gene="menA"
                     /locus_tag="IW715_002273"
     CDS             complement(151..1104)
                     /gene="menA"
                     /locus_tag="IW715_002273"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1022863.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1117..2220)
                     /locus_tag="IW715_002274"
     CDS             complement(1117..2220)
                     /locus_tag="IW715_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1022864.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2310..3245)
                     /locus_tag="IW715_002275"
     CDS             complement(2310..3245)
                     /locus_tag="IW715_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1022865.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3547..5481
                     /locus_tag="IW715_002276"
     CDS             3547..5481
                     /locus_tag="IW715_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1022866.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5938..6354
                     /locus_tag="IW715_002277"
     CDS             5938..6354
                     /locus_tag="IW715_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1022867.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6368..6934
                     /locus_tag="IW715_002278"
     CDS             6368..6934
                     /locus_tag="IW715_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1022868.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6957..7943
                     /locus_tag="IW715_002279"
     CDS             6957..7943
                     /locus_tag="IW715_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1022869.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8098..>8574)
                     /gene="scm"
                     /locus_tag="IW715_002280"
     CDS             complement(8098..>8574)
                     /gene="scm"
                     /locus_tag="IW715_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1022870.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2521 a         1658 c         1738 g         2657 t
ORIGIN      
        1 cgactttttc acaacctcct gataaacggt gttcaaaagc ttgcatctgc ggtaataagc
       61 ccaaccaaac gaaaaatata aggagctatt tttatttggc tgttcttatt acttgatgca
      121 gggcagtttt ttcacaacct cttcatgctg ttaaatggct tgcataatga ttgttagtaa
      181 tagccccaat acataactgg cattgaataa gaccatattt ttgatggaat agctgaaact
      241 gataggctgg ggtaacgttt gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt
      301 caagaaggtg accaagatcg gccattcata gataccgctc aacaacccca ttaaaatcac
      361 gaaatagcaa gcatacatca atagctggaa taagactgtt cccgcaggac gtccgatata
      421 aaaaactagt gtatagcgat gattttcaat atcacggtca agatcccgca aattattagc
      481 caacatgata ttggcaatcg taaacaccaa gggtaaggaa gctaatacga ttgctgctat
      541 agcccaaaga tttcctacca aatcaaattg tccagatgaa aagttgactg tcagatcata
      601 gacatctgaa ccgaatgtat tgacataaac gaccatggca aaaattccaa ggcccatagt
      661 caccccactg aaaatttccc ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata
      721 gaatactcca ataaaacaac agataaatcc catcaaaagt aaaagccaac ccgtattgat
      781 cgttagaata acgccgataa caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat
      841 caatacagga gatagatttc tgcggccaat gacattttct tcatacttat atacttcaga
      901 atgagccttt tcataatcca tgtaattatt gatagcggta gtcgctaaat caaaaactag
      961 cattccaata aaaaataaaa aagtgtttag ccaatgtatc tcatgaaaaa aagcgataga
     1021 gaataatacc cctaccgcaa aaggaaatac actagccacc tttgttcgta tttcaactac
     1081 ttctaaaaag atttttagtg tcatggttcg tactccttat tttagttcag aacggtcacc
     1141 catcgtgtaa tgggagtcct ccgataattt gaaggtgtct ttgattggat cggtcacata
     1201 tactttatcg tcttttgtca cgaagatcgc ttccgtcccg ttattcaatt cgctttcgac
     1261 gtattccagt ccttttttca cacccattga aaagactgct gtagacaacc cgtcaccatc
     1321 aattgattta tctgttatga tcgtgacact tgcgatatca ttgtcgaacg gataacccgt
     1381 ttttggatca aacaaatgat ggtatttttt gccatctact tctaaataac gctcgtaaat
     1441 tccagaggta actaatgttt tgttccgttc tttaatagaa cctaaaacag atccgcgagc
     1501 catgttcgga tcttggatgc cgactgtcca gtcttggttt tcgcctcttg ggctgtggcc
     1561 taagacatag acattaccgc ccaagtcaac gatggcagtg gtcactcctt gttttttcaa
     1621 gactttcact acttcgtccg taatgtaacc tttggcgatt gcacctaaat cgatgatcat
     1681 tcctttttct tttagataaa cagttttttc tttgtcattt agttcgatct tatgataatc
     1741 cactaatttc aatgcctgat cgatttcttc ttgggaaggt tttctggcgt catcaaaacc
     1801 gattcgccat aattgtgtga ttgcaccaat cgccatatcg aatccgcctt gagaatcttg
     1861 gctatattcg taggcccgct tgactaacgt gtagacgtca tcagaaactt ttactggttt
     1921 gatcccagct tgctcattca cttcatcgat ctcagaacct ttttgattga tcgtgatttt
     1981 atcaccaagt tcttttactc gatcgaaggc aggtttcaat gctgattctt tgccttcatc
     2041 atacacacgg atacgaacgt aagtccctaa caagaactgt tcatcagaat atggatcttt
     2101 caatatcttg gtagctgctg aactttcagt ggttgtttta tttgctgagt tgctgcagcc
     2161 agttagcacc ataaaagaga tgcccagtgt gattaaggaa aatagtagtt ttttccgcat
     2221 ctttttcctc tcttcatttt gaaaggtgag acgactgcct aatctggcta tcgtcccacc
     2281 ctccaacttt tattagtatg attatgtgat tatttttctg ttacgatatt atctacttcg
     2341 attgtttgag tgtcgccttt ttcagctgca ttcactaatt gttgtgcgta catgatgaat
     2401 gaatgtgagc tgtgtgtagc acctgtaaca acttctacat tagcaggtga gccatcttct
     2461 tcgcccattg ctttgacgaa ttcgtcgttc agagtaggga tgtaagtttt tggttctgta
     2521 ccgctttttg ctttcatatt ttcattatat tctgtatcat cttgttttga tttaccgtct
     2581 ttgtcgatat aatcaaagtt tgattcagtt actttgccgc cagcaactgt catttcgaat
     2641 tgcacacggt agccgttaga gtagttcttt tcttcaagct tgtaagtgcc gtctttcaaa
     2701 tcagcaccat tgtcgatttc gattgtatct gtgttaccag cttgagcagc ttggatcaat
     2761 tgttgtgcgt agttttggaa gctttcagat gagtgagtag ctccagttac gacttccaca
     2821 ccgcttgcgc tttgtgcttt taagaattgg tcattcaact caggaatgta tttttctggg
     2881 tttgtgccag attttgcttc catgtttttg ttgtattctg catcttttgt ttttgattcg
     2941 ccgttttcat tgacgttatc atattttgat tcagtgattt tgccgtcttt tacaaccatt
     3001 tcaaagactg cacggtaacc atttgaataa tttttttctt ctaatttata tgtgccgtct
     3061 tgcaaatctc cgccagctac aacttttgca gatgattctg ttgtgctttt tgcagtcgaa
     3121 gattgtgctg tagatgattc agaagaagag tttgagcttc cgttgtcatc tgcgccgcaa
     3181 gcgcctaaca ctagagctga aaatgctaat acggcaaatc cagttacaaa acgttttttc
     3241 ttcattttaa taccccacca ttccatttct agtccacaat ttttctttgt gattcattat
     3301 acattctaat ctttttagcc tataagtcaa ctatttttga taaataaccc atgtaatatt
     3361 tcaaacgttt tctttcagca aatttcgtga aaaaacgctt tattttaaaa tttgaaaaaa
     3421 atccaaaagg agttagacaa aagaacagaa catctatata atatatggag attgtgtaat
     3481 aagtatcaaa ctaaaagaat tatcatatat tcattatata gatagtctat aaggagcgga
     3541 tgatcaatgg caaagcaaaa tattgtcgtt gtaggagcag gatatgccgg tgtttcagct
     3601 acaaaatact tggcaaagaa actgaaaaaa gaagatgtag caattactct gatcgatcgt
     3661 cattcgtacc atactatgat gaccgagttg catgaagtag ctggcggacg tgtagaacca
     3721 gaagcaatcc aatacgatct gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc
     3781 acagacactg taatcggtat cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc
     3841 tatccttttg atcagctgat ccttggaatg ggcggagagc cgaatgactt tggtacacct
     3901 ggtgtcaaag cacacggatt caccctttgg tcatttgaag attctgtaag gattcgggaa
     3961 catattgaga agacagtagc aaaagctgca ttggaaccag atgatgaact gcgtaaagcc
     4021 atgctgactt tcgttgtctg tggttctgga tttaccggta ttgaaatgat tggggaactg
     4081 atcgactgga aaaatcgcct agccaaagat tataaattag atccaaacga aatcacactg
     4141 atggtcgtag aggcaatgcc gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa
     4201 gctgaacgct atttgcgtaa gcacaacgtg gaattattat tgaatgcacc aatcgtagaa
     4261 gtggctgctg atcatatcaa attgaaagac ggacgcaaca ttccgactca cacactgatt
     4321 tggacagctg gtgtcaaagg aacttctgac gctgctgact ttggattaga atcagcacgc
     4381 ggacagcggt tgatcgccaa tgaatatatg caggcaaaag gctacgaaga taaaaacatc
     4441 tatatcattg gtgatttagt cttttatgaa gaattcccag gaacaccgac accgcagatc
     4501 gttcaagctg ctgaacaaac aggacacaca gccgcagcga atatcgtggc ttcgatcaaa
     4561 ggcggagaaa agcataaatt taaaggaaac tatcaaggat ttatggtttc tattggtgcc
     4621 aaatgggggg tagctaacct atttgataag atccatttaa gcggattttt agctatcgta
     4681 atgaaacata tcgtgaattt gaaatacttc tttgatattc gttctggtta ttatatgttc
     4741 caatatttaa tgcatgagtt tttccatatc aaagatgatc gcaacgtgac tcgaggacat
     4801 tcttctcgtt acggcaatgt cttatggagt gtgccacttc gtattttcta tggtttagtt
     4861 tggttcgttg aagcttccaa aaaaatcatt gggaatggtg aagtgttcaa accaagtact
     4921 tggtttggtg aaggatcatg gttcactgga aacagtgtgt tcccttggac atgggaatac
     4981 aatgtaacag atgttgccag tggtgcttct gctgctggtg atgcaaccac agctgccagc
     5041 ggagcagggg caagtgccgg agaagcagcg actcaagctg cacattttgg tttgagttat
     5101 gcatacggtg aagaaccaat ggctgtgatt gagaagtcac cagactggtt cacaaagatc
     5161 atgcaagtga tcatgccaaa caatgatgta gcaatgttct tccaaaaatt catggtattc
     5221 tttgaaattg ccttggcatt agcattgatt gctggattat tcacatggct atgcagtgcg
     5281 acaacgattg tattggtgat cacgttctgc ttatcaggca tgttttactg ggtaaacatt
     5341 tggttcattt ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat
     5401 cgctgggtaa ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt
     5461 tcccagtacg gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag
     5521 aaaggggctg agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg
     5581 cccgagataa gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt
     5641 tataaacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt
     5701 gaaaagtaat tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc
     5761 tcttgttata aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa
     5821 acatggaaag ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac
     5881 aacctcttgt ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg
     5941 agccttaaag aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg
     6001 cttttgactg ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca
     6061 acagcagaaa aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg
     6121 atggctggag aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg
     6181 atcgagatag atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc
     6241 gtacgtcgag gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta
     6301 gtgatcgaag tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc
     6361 acacaccatg agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt
     6421 gatcattggt ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa
     6481 actaggctta gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag
     6541 cttcttacta ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac
     6601 attcctttat agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca
     6661 attaggacca aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa
     6721 tgtcggacag cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt
     6781 gccagtcctt tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta
     6841 tcttttacga catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa
     6901 acaaaaatgg agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga
     6961 agctacatcc aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa
     7021 aacggatgga agaaactgtc cgcctgaaaa acaaacctgt cgaacaagca atcaaagaaa
     7081 cgatccatgc cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg
     7141 gaccagagca agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata
     7201 cggccacact gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta
     7261 atatccgtag tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct
     7321 gctgttttaa actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc
     7381 gcagtatgga aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg
     7441 aactttctgt aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat
     7501 taagttgttc tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag
     7561 gagatatcgg actttctatc gggatggcct ttcagattat cgatgatatt ttagattata
     7621 caaaagagag ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt
     7681 ctttaccgtt gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga
     7741 agaaagcggc aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag
     7801 gaggcgtgga agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa
     7861 taaagaaatt acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac
     7921 tgattttgga tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga
     7981 aggaaatcta cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc
     8041 aaaaataaag agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta
     8101 ccttttcgat ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac
     8161 gcctgcgagt gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga
     8221 tttgtttcca ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga
     8281 acttgttttt gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc
     8341 tgtactatcg cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt
     8401 actgtcactt gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct
     8461 atcacttgtg gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc
     8521 acttgttgtg gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgct
//
LOCUS       DACEMK010000103         8425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEMK010000103 DACEMK010000000
VERSION     DACEMK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.106"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            472..2109
                     /locus_tag="IW715_002281"
     CDS             472..2109
                     /locus_tag="IW715_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1022871.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2254..2994)
                     /gene="truA"
                     /locus_tag="IW715_002282"
     CDS             complement(2254..2994)
                     /gene="truA"
                     /locus_tag="IW715_002282"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1022872.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3185..3820)
                     /locus_tag="IW715_002283"
     CDS             complement(3185..3820)
                     /locus_tag="IW715_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1022873.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3967..5208)
                     /gene="sstT"
                     /locus_tag="IW715_002284"
     CDS             complement(3967..5208)
                     /gene="sstT"
                     /locus_tag="IW715_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1022874.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5406..6402
                     /locus_tag="IW715_002285"
                     /pseudo
     CDS             5406..6402
                     /locus_tag="IW715_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6418..>8425
                     /locus_tag="IW715_002286"
     CDS             6418..>8425
                     /locus_tag="IW715_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1022875.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2786 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aaactaatca gaatcgtaca aatatttaat aaccaaatat atagaacata acttaatatt
       61 aagttatttg aaattccata aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa
      121 tagttcagtc catctccata attgttaaat gttgtatagt tgattggccc ttcaaaaata
      181 gaagataacc ctttgattcg tgaaagatga aaagataaat cacctgttgt ataaatttct
      241 tgagcatttc tcataaaatt tactgaatat aaagtactaa gtaagataag aactactaaa
      301 agaaatgctt ttttacttat tttcatagat ttgttgttct ccttttttaa ttctattttc
      361 agtatactag aattgtcttt ttttaataag ttttgcgtta aatttttcat agcactagta
      421 tgatatacta gaaaagaatc aatttttatt acttttaaat aggagaaaaa aatgaaaaaa
      481 aacaaaaacc aaataataga tatcattttt atgttatttc taagttttgc ttacgtcatc
      541 cctctattca tgtctatggg aatctatcat agtgtcaatc aggatactta ctttcattta
      601 tctcgaatca taggactaga taatgtatgg agttctccag taaacttcaa taacttcgat
      661 catcatggaa caatgatgaa tattttttat ccatggctta ctctttatcc agctttttta
      721 ttttataaaa tgatgggaaa tcttgtttta agttataata tctactattt ttttatcact
      781 tttttaacaa tggtcgtttc ttatttttct atgaaacaaa taaaaaacaa tcgttatatt
      841 tcgctgttat tttcgattat ttatacattt tccgcctatc gtgccattga catattccat
      901 cgtgcttcat tgggagaagc tgtcgcttta acatttttgc ctttgatact aatggggtgt
      961 tacgaaattt atattagaga ttaccaaaaa tggtactggt tgtctattgg catgacgcta
     1021 gtagtgtata ctcatttact ttctgtcgct atggtttctg tatttattgg aggaacgttg
     1081 tttctttctt tttatttttg ggaccaaaaa atcgctagac tcttaagtct tttaaaagct
     1141 accgtcctca cctttttttt aagtgcggga ttcttaattc cttttattca acaatctaga
     1201 gcacaagaat tgaaagtccc actgggaaaa gaattaagtg gtatggctcc tagtgatatg
     1261 ctgacacaca ttttaaataa taactataac aactatacta ttggattatt cttatttctc
     1321 ggattgattg gtgcttttat ttttataaaa aaattaactg ctgatgattt atttattttc
     1381 tttcttggtg tatttgttct tttttgttca accaatttat ttccttggca actttttaac
     1441 catacaccag ttaagagtct acaatttgtc tggcggttaa atggtttttc ttctttattt
     1501 atagcctata cgatgagtat agtgatctat tatatttttt ctactaaaaa taattcatgg
     1561 aaaataatgg tctttacgtt tcttgcacta atattgcatc tctcaggagt aagcaattct
     1621 atccatgcta acaaagattc attaacactt atagcacctg aagacgctgt tgctatagct
     1681 gaaaattaca atcatactga ttatgcaaac aaagagtcaa tctatcatcc tgatatgatt
     1741 aataatgatc aatatttact aggtaaccag gaaataaatc caacaactca ttttatgagt
     1801 aacaaactta caattgaatt agataattcg aataataaaa atactgtctt gacgacacct
     1861 atttatcgtt ataaagggca agtggcttct attaatggca aactagtaca aacaaaatta
     1921 tctaagtttg gcacaactga attaactatt cctccaggta taaataaagt cgttattact
     1981 tatcagtaca caaaactagc aattgcttca agatatttat ctatcgttac tttgatttta
     2041 tttttacttt atcgatttac tttttctaaa caaaaacaac cgagaagaga agtacaacat
     2101 tctcactaga tttttattta agaaagactt tttagctgta cacataaaca ggctaaaaag
     2161 tcttttttta tatttcttta atttgcttca tacattattt aacatcgata tttatactgt
     2221 gaaacatgcg tttcatccat tatcaaagta tttttatcga taataaactt catccaaaaa
     2281 cagaccttgt gcagggacgg tttctcctgc atgttgccgc actttgcttt caaatatttc
     2341 atcgattgtt tctatcggca tggcacctgt gccgatttct agtaatgtcc ccatgataat
     2401 ccgcaccatt ttatataaga atccgtctcc gacaaaagta aaatgaagca tttctccctt
     2461 tcgttcgata gataattcat caatcgtacg cactgtggat ttttttgatt ttttcaatgc
     2521 agaaaaacca atgaaatcat gtgtacccac taatttttcg caagctgcat tcagcttgtc
     2581 catatccaac tcttgaggga aataaaagct gtggtttcgc tcaaatgctg aaggaatcac
     2641 gttgttccaa acataatagc tgtatttttt gcccaccgca ttatatcttg aatggaagcg
     2701 ctcaggcatt tcttcgattc ttttgatgac aatatcccgc ggaaggtaac gattcataaa
     2761 gtcgatcatt tctgccagtc caatttctgt gtttgtttta aaatttgcta cttgtcctcg
     2821 cgcatgggtg ccagcatctg ttcttcctga tccaataatt tctatctctt catttgtcat
     2881 ctgatgcaat actttttcga tttttccttg aatggtttta tcgtcatctc caagtctttg
     2941 ccatcctgaa tatctttttc cgtcatattc gattgtcaac ttgatgtttc tcatatttta
     3001 gtggctcctc tgtcttccat ttggtcttct ctctaccttt ttaagattgt agcacaaaat
     3061 atagctcctt tcttctctaa aacagaagct atacaagaaa actgtggaaa tatatatttc
     3121 agaaaaacaa aaaacagcca ccggatgaag tatccgctga ctgttcaatt tgacggctca
     3181 agttttactg tttgagatgc aagatactca accaggcaat gggcacataa ttaaagaaaa
     3241 aactgattct ttggcaaaaa cctattttat tcagtaatgg ccaagaattt atagaaggaa
     3301 ttcttgccag tccataaatc gccgcaaaaa gcaaactgat cacagacagt ccaaggtata
     3361 agtggcttac gtccatttgc ccattttttc gatagatcaa gaaaagaaga actggaaaca
     3421 ataaaaaacc actgtaacct aaacctgatc cgatattatg gacccatgtc gagacattcc
     3481 acgaagattc atgggtattg atactgaaca aacctgtaaa gatacaatca cctataccat
     3541 agaacgcgat ggctgctgta actagggtag ctaacacacg agatgtactt acaaatgcag
     3601 catacaatgc aggcaatgac aggatgaaaa agatccctga aaccacagac caaataagaa
     3661 acgcttttct tacaggactg gaaacctctc caaatacact gatcacgctc ttcatttgat
     3721 ctaattcagg ataatacaat cctaatacgt acggagtcaa aaaatcactc agcacgccta
     3781 ataataaaaa gaaaaaacca aatttacgta aaaaatccat gatacgcctc caataacaat
     3841 tacctatgct tttaattgtt attataagcg actttatctt gatttaacag aaacctctat
     3901 ttacacgaac atctatataa ctcattaaaa actcgaatca ttttaaaaat actaaatatc
     3961 taacgttcaa cgttcactta atccatctaa acaatctttc aatttgattt tttccccact
     4021 tcttttacgc gcgcctagct ctcctattgc agtaaacaac agatcacttg acgaatttaa
     4081 ggcagtttcc attgaatcct ggataacacc gataataaat ccaataccga cgatttgcat
     4141 cgcgacatca ttagagatac caaacaaact acatgctaat ggaatcaata acagtgaacc
     4201 gcccgcaaca cctgaagctc cacaagcagc taaagcagac aaaatacata aaaggacttt
     4261 caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca gtcaacgtca tcaaggaaat
     4321 ggtaatcgcg gcaccgccca tattgatcgt agcacctaac gggatcgata tggaataaga
     4381 ctcttttgtc aatttcaatc tttcagccaa catcatgttt atcgggatat ttgcggcaga
     4441 acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa caaaagaaaa tcaatggata
     4501 gggattttct ttaataaaca aaaacgtgag gaatgggtac acaactaatg cgacaaacag
     4561 catcgtaccg atcaaaacaa gtaaaagctg cagatactct gccaatccag ctattccagt
     4621 cgtagcaatt gattgataaa ccaatcctaa aatccctatc ggagcaaatg caataatcac
     4681 ttgtacgact tgtgacaacg ctatagatat ctgatccacc aactcttttg tcgattctga
     4741 acgtgctcga aaagctagtc ccaacaatac agcccagaat aagacgccaa ggtaattcgc
     4801 atcaataaga gattgaacag gattttgtgt gacattcgtc aacatagtag ataaaacact
     4861 ttccaaactt tgcggtgctt tttcagatac tgtttcttgt aaagtaatag gaacagtaaa
     4921 aattgaactt acaataactg ctacaagtgc agaaagcaat gttcccacta aatataagat
     4981 caaaataggc ttcacgaatg tcttattccc aattttatgc ttggcaattg acgccatcgt
     5041 caagaaaaaa accagaatag gtgcgattgc cttcaagcaa ctgacaaaca acgttcccaa
     5101 tattgaaata aatgtccatg tggggaaaaa gataccaaac aatattccga ctaaaatacc
     5161 aacaacaata cgttgaacta acgacatatc caaaaacttc tctaacaaat gaccaccctt
     5221 tcacaatttt aacaatcatt gtatcatttt atgcaaagaa actaaaggta taattttcta
     5281 aaaattctaa caatacaaac aaccatttct tgctaatttt gtactttttc tgtctacacg
     5341 ttgttttttg agatattcta agttaggatg ttattaatgg gcaacttatc aggaaacgtt
     5401 ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt cttgaaaaaa ataaagaagg
     5461 agagttatgg cttcctgaca atctttgtcg cttgtataat gatggtttta cagcgtatat
     5521 tgatttcagc agtcactatt cctacatatg caacaaaccc tggtcgattc tacatctata
     5581 catttttaca aacttccgtt gttattggtg caaatttgat cccactttac atgggttacc
     5641 aatatcaaaa aatcgcccgt ttaaaagttt tacattattt aagtcgtttt gcctttatat
     5701 tcctaatagc cacactagtt tgcaatatct tttttttacg tacaagctgg cagcttgaat
     5761 attcgtgatt attggcttat cctaacacct atcagtcaga attactttac ttatgctgtt
     5821 tcttgtactt tgcttttttg tagtattcct tatacagttg gatttttaaa taaattatcg
     5881 aaaacaacaa ttaaatatct actattattt ttaacaattg gattagttgg ctgctctact
     5941 ctatttaata aagacccgtt tgcattacag aatggacaga gtattttatg gattttttat
     6001 ttatttttaa tgggttatgg tttgaaagaa tggaactgga aagaaaaggt ccgctataag
     6061 cttcctcaag tatttatcag cttgattgtt ttattcggtt tgattatttt gatgacacaa
     6121 atttcactca tcatcagagg aaatacagat acagcgctga ggttttctaa accatattct
     6181 ctcttcgcaa tgtattatac tttctcttcg tttttatttt tagaaatgct atctgataaa
     6241 ttaggtataa aaggaagagg aaattttctt gctgtatttt tgattgtcac tcaagttata
     6301 tgtaatttcc ctttagtttc ttatcgcgta tctacattta tgaaacgtga atttccaaac
     6361 tcagggaagg catggctgat aaatatcggg cagtttttta tttgctattt attagcagtg
     6421 tccctcctag ttatcttttt attttatata caaaaaatcc gtttcattca tcgtttcatc
     6481 gaatacttcg cttttgattc gattgaacaa ttaactcagc gttggaaaaa aagaattcat
     6541 tggctttttg ttcataaaag ttatttttta gttgcagcat ttttttactg ctttaccttc
     6601 gtgcagattt tcgttttaga accaaaagaa ggttggaaag aaactatcca ggttgcctta
     6661 aaaatattta ctcagcgaca agcacccatg atcctgacaa tcatcattat tatgggattt
     6721 ttcttcttac tattgttgat ttccaatcgt ttttggtacg ccttgactgc taccttgatc
     6781 attaacttac tgctcactat ttcgacagta atcaaaatgg aaatgcggga agagcctgtc
     6841 tttccttcag atttaaaaat actgaccgga ttgtcagaac tattatcgat ggttagccca
     6901 gtcttattaa tcgtgggcgg attgatcctt ctcctattat tgattactag tattattgta
     6961 caacgtagac tacaaaaaca gtattccttg aagattcatt ggaaacgtcg atttatcagt
     7021 atcgctgtat tattaggatg tttttcgggt gtctttttca ttaatcacaa aaattcgcct
     7081 tctttcttgc tgtttaattt atttaaagta aacaagactt tctttaacca gcaaggcgcc
     7141 gtaaaagata acggaccagt cattcaattc ctaaacaata tcgacattaa aatcatggaa
     7201 gaacctgctg gctattcgaa agaaaaaatt aatggaataa tgaaaaagta caacacaaag
     7261 gcagatgaaa tcaaccaaac aagaagtgac tggttaaaca atcagacgat cgttctcaat
     7321 ttaagtgaaa gcttcagtga tcctgcacgt gtccctaatc tgacagtacc tacagaccct
     7381 attccttaca tcaaaagcat tataacagaa ggaacaggag gattgatgtt atccgtcggt
     7441 tatggtggcg gaacagctaa catggaatgg gaaggactga caggcttgag tatcagtaac
     7501 ctgtctgctt cgttagttac accttatacc cagctagtgg agcgtcagaa aataagtcca
     7561 aatattaccg atctattcga tgaaaaaata gccatccatc cttttacagc tgctctttat
     7621 cgcagaaaac aagtgtttga taaatttggt tttgacaggt tctattattt agacagtccg
     7681 gataaattaa cttatacgga taaaatagaa caaagcccac gcatatcgga tgattcagca
     7741 tacaaagaaa ccttgaaagc tttacaaagc aatacagaaa cgtcacaatt catccaatta
     7801 tccactatgc aaaaccatat gccttatgat gattactata gtgaattgaa ctacactgct
     7861 gaaggaactg ctgtcattcc taatagaaga aaagaattac aaacttatat gcaaggcctt
     7921 cactataccg atacagcagt aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg
     7981 attacctttg ttttttatgg tgaccattta cccgcaatct atagtgggaa tgattcgaaa
     8041 aaatatggat tagaacagca ccagacagat tatttcattt ataacaacaa gtatagtcgt
     8101 gaacaagcag ctacgttaaa taaagaagtt gttgctccta ataactttgg tgctatggct
     8161 cttgagcatg cgaatatcaa agtaactcct tactatgcgt tgttgacaga agtatctaat
     8221 catctacctg cattaacaat tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa
     8281 caggtctttg ttacagagga tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa
     8341 aaagaaattc tagaagatta tcgtctgata caatatgatc ttgttgcagg taaccagtac
     8401 agtgcaacat gggcgacaca aaaaa
//
LOCUS       DACEMK010000104         8424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEMK010000104 DACEMK010000000
VERSION     DACEMK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.107"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            351..1790
                     /locus_tag="IW715_002287"
     CDS             351..1790
                     /locus_tag="IW715_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1022876.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2085..2303
                     /locus_tag="IW715_002288"
                     /pseudo
     CDS             2085..2303
                     /locus_tag="IW715_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2552..2767
                     /locus_tag="IW715_002289"
     CDS             2552..2767
                     /locus_tag="IW715_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022877.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2984..3240)
                     /locus_tag="IW715_002290"
                     /pseudo
     CDS             complement(2984..3240)
                     /locus_tag="IW715_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(3427..4338)
                     /locus_tag="IW715_002291"
     CDS             complement(3427..4338)
                     /locus_tag="IW715_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1022878.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4357..5361)
                     /locus_tag="IW715_002292"
     CDS             complement(4357..5361)
                     /locus_tag="IW715_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1022879.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5358..7481)
                     /locus_tag="IW715_002293"
     CDS             complement(5358..7481)
                     /locus_tag="IW715_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1022880.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7486..8406)
                     /locus_tag="IW715_002294"
     CDS             complement(7486..8406)
                     /locus_tag="IW715_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1022881.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2418 a         1716 c         1556 g         2734 t
ORIGIN      
        1 aatgtatttg aaaagcaaat tggaaaacaa ataggtgcaa caccataaat atgaaaaaat
       61 tgggaaaaag tagaatggaa atttgcttac gtctcacgag atagctcaca ctattaatct
      121 gaagaacaat gaagtcgact tttagaaatt gctagaagaa agtaaagcga attatcacta
      181 gttactatag agtaagttca aataaagcat ctccaactta catggttaca tgaggatatc
      241 aaatcgaaat tactcaagct tcctatggct agagtcgcga gaagttgttg ggcagtagac
      301 ataggttata ttgttcttag ctacacgatc attagaaagg aaatcctatt atgtatagtt
      361 atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat tttgattgct atatctatgt
      421 ttaccttcat gtccacattg gatggcagta ttgtcaatat tgcactacct accatctcag
      481 atgatatggg cataccaatg aatcaagcgg aatgggtagt gtccgtttat ctgatgattg
      541 tctgttcttg tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa atcaaagtat
      601 ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc aatcattcgc
      661 taggtatttt attgttcgca cgggtggtac aagccattgg tgctagtatg actatggcta
      721 caaatgccgg aatcatcacg gaagtctttc ccatacatga acgtggtcgg gcattgggag
      781 caataggtgc gtttgtttca ttaggctcga ttgctggacc aggtcttggc ggcttgattt
      841 tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt accggtggga atcgtaacga
      901 tgttgattgc tgaaaaattc ttaccaaaag atatcaccat gagcggccat acaatcgatg
      961 gagggggctt tgctgccatt gcggctacga ttatcagctt ttttggagca atctttttgg
     1021 ggcaagaaag tggctttttc catccagtcc cccttgtact gttcgtaatt tcagtgattg
     1081 cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc tccattgatt tctttcggta
     1141 tttttaagaa caagattttt accatgagct tgattacagc cttgttgatt ttttcttcca
     1201 acttctttgt caatgtagtg attccttttt atctgcaaaa tgctcgcggg ttgagtccta
     1261 gctatgctgg gctgttgatg atggtctttc ctttgatgat ggtcgtcggg gcgcctttaa
     1321 gcggctacct gaccgaccgg gttggcccgg agattttggt attagtaggc ttgggcttat
     1381 tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg tggctataca
     1441 ttgtcgctac tgccatcatg ggtgccggca atgccttgtt ccagtccccc aataatacga
     1501 tggttatgag ctcggtcact atcgaaaact tgggactggc aggctcgatg aattcctttg
     1561 cccgaaattt ggggatggtc agcgggattt cgctttctac gacggtctta tatcaagcga
     1621 tgtctgctaa gatgggccag caagtaacta actatatcgc aaatcggccg gatgtctttt
     1681 tatatggaat gaagattacc tttttaggca gctttgtttt gtgttttgtt gcctttttcc
     1741 tgactatctg gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga aaaaaccact
     1801 gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga tttccatgga tgatggaaga
     1861 tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac tagtgctcaa
     1921 tgagatgctt gatttgatca caagaagact gcgaaagccg ctggcaaccg caagctaggt
     1981 atcccaccga tggactacgt tttagaggct actggttatg aatacggagc caacgctctc
     2041 atctgcataa gaatacatca cccagattat gtaaagattt tcgatacttc tgtcaaaaaa
     2101 tcattaagat agataaatga tttccgtcta gtcaaattgg ttcagaatgt agacacacag
     2161 atggtaagaa gtcactcaca attcgagaat ggacttgtcc tatttgccgt atacatcata
     2221 atcgaaacgt caacgatagt atcaatattt tgaccaaggg ctatgaatac aagcattgcc
     2281 ttagaaaaca tcaagaacct taggaacaac ggggatagct tggttaataa gagataccgc
     2341 tgtttaggct tgcaagtata ctcttttatc aagaattccg tggctttagt catgagaggc
     2401 ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct aattataata
     2461 agatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta ccattttcaa
     2521 agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg atatgaattt
     2581 aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta ctggtatcaa
     2641 tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc tgaatctttt
     2701 agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga ttgaaatatg
     2761 gagataatta tcgtctagtg aattacttgc cacttagctg aatttaacga tccttggtgc
     2821 ttgaaggggg agattcttgg gaatagagca tacttgcaag cctatgtctt tactaacaac
     2881 gatatctctt attaaccaag ctatccccgt agttctaagg cttttacttt tatctaagca
     2941 ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc agacgtgagc
     3001 ttaggcattg ctgaattttg ttaattcggt tttaggttgt gctttgagca taatatgaaa
     3061 gtattcttta tcacgatttc attcatctaa ggtaatgtga tgtgattctg aaattctttc
     3121 aaaggcagtt tttgcataag ctgacattac atcatcaatg acatgtttac aatgtttcac
     3181 tactaaaata agggggtaac caagaagaaa aactgaatga ttatttgtat ctaattccat
     3241 ggtatatcaa ccatttccta tatagactga ttatgacatg gattaaaata aaaaggcact
     3301 atgtcttaat tgtcggtttt cgagttaccg aatggcattc atcacccacc tatagaggat
     3361 gggcgacttc tgcctgaaat aagttacact acttataact tctaataaaa ttagattgag
     3421 tcattattat ttaacatcaa cgattttcca gtttccattt ttttgtaata ctaatttgta
     3481 ctgaaagtag ctagtggttt ttgatacatt atcaaggtat tttatctttg tactcactag
     3541 aaaatttttt ccgttttgta gataaactgg attctctaac tccgaaaact ttaagtcaat
     3601 attgattggt cgcatcgcac cattttcaac ataatactcc aattcttttt cagaagctga
     3661 tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg ttttggcatc
     3721 aatttctgaa tcgttttcta cttgttttgc ttcatatttt gccttgtctg gctttccaat
     3781 cattgttggc aaactagtta ctaaatgatt cctgttttca tcttcaaaga ttgtcactcg
     3841 atatgcagaa gaaattgatt gcgtcttttt tccctctgta atatgttgct ccactaaaaa
     3901 tgaaacgaca aattcttccg agtgtttttc aacatctaga atttttacag attgaacttc
     3961 ggaactcgtg ggaatatctg ctcgaaccat atcttggatt aaagcgagac cgttttctgt
     4021 aaggtactgt tcgagattac tcattctctt ttcaattgct tctttgttat ttttccatga
     4081 aaaatattct ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat tcactacctt
     4141 ctcttttata accacttttt catgagttgt atgttgatca atggcggtga aatttttata
     4201 aacgccaaaa gcaagactac aagacaataa aagccaaagg gcaagcacca tttttcgatg
     4261 cttacccacg gatacaactc gttctttttt cttttttggg ctcttttcct tttgattccg
     4321 ttcaattttt atcttcattc tttttcctct tttctcttat ttgtatcggc cagctccgat
     4381 aagatgctgc cgccaatagt tttccgttag attcgtccac cccactggat ctcctgcatg
     4441 aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc acataagtac cggcatcata
     4501 ggttgaatga aagaaaacta aatcacctgg tttggcatct tttagatcaa tatgagtcat
     4561 ggcatcatat tgatcctgag ccacccgagg aagtttcagg tcaactttgg cataacacca
     4621 ttgggtcaag ccactacaat caaaccccgt ttctggactt gagccaccaa acacataggc
     4681 cgttccttca tacttaaatg cctcatcaaa aatttcttgg actattttgt catcaaactg
     4741 agttgtagtt aggtattgct tcactaataa acagtaaaac atattgccat aattataccg
     4801 ccacccacca ttttccttaa tcgcgattgg gttggggtaa tcgacttttt gaccacctga
     4861 tttttccttt gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt ttttcccgac
     4921 atgatctaga tacccgccac catagttata ggcctgaata atactatctt gatcacaccc
     4981 tagttctttg gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt tgatggatgc
     5041 ctcggtactt aatgaattag gtggtaatcc ggctgactca gaactttgca taacgtcttc
     5101 cattgtgcca ccagattcca cttgaataat cgccaaaaga attggaatat actctgttat
     5161 atccgattct ttggcatact tttccaccat tgcacggtgt ttcaatactt cttctgagac
     5221 actagttccg ccaggagaag tgccagaact accatttccc tcactgtctt caccaaacaa
     5281 caaacccaca cacatcaaaa tacccataaa agagatggtc agaaaagtta atgaaataac
     5341 acccactttc ttcaagttca ttttttctca cctgaccttg attttggacg atggttcatt
     5401 cttttttgta ctttttgatt ggtctgcttt ggcagagtgg acctctgttt cacttgttgg
     5461 aaagaatttt tcttacttgc tgattggctt ttcccttttg aaatgatttc ttgactagtt
     5521 agttttcgtt tgatttcggg attcagattt gctctcacaa tccgctttgg tgcttctttt
     5581 ttcagcatca tatctttctg aggttgagct gctttatcta ttggaatatt cgcccttctt
     5641 tttccctgac gatttgttgc ttcactcagt atcttgcgtt tttcgtccat tttcgcattt
     5701 cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg ctttttcttg gaacagtccg
     5761 cgcttgaatt cttctggtgc ttttttcgac ttttcgattc ctttatggag attgtatttc
     5821 aaattcgttg gcgtattcct tacctgatcc tttacttgtt tagtcttatc tttcaccaaa
     5881 gcttgggtat ccaaaacttt gccgattttt ctcccagcca aattcattcg ggattgtttc
     5941 ttattatttg aaactggatt ctccttagtt aagtcagata gtatttcgtg atcgttcggt
     6001 aatcgtgaat tctttcgtag agaagaaccg gatggcttaa atttggattt tgattgttcc
     6061 tttgccaact tatttccgac tgctgcacca ccaccaaaag ccaaagctcg tcccacattt
     6121 cgattgagtt gccgcaattt tcgattcatt aacatttgcg gttttcgcat gacgcgtctt
     6181 cccattgact ggctatcatt actctggagg ttgaacatac tcataatgtc gcccaatttg
     6241 aaatagatac cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa tggataggtt
     6301 gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac caaagtaaca
     6361 cccgctcgca tcataatcgt attgaataaa cgaataatcg ccttctttcc taaactttca
     6421 taagtcggca acatggacaa taagaaacta attggcagaa acatcgcaaa gataatgaag
     6481 agaatttgcg agaacaacat aatccctgtt agaagaaaaa caaagaagga aatgccaata
     6541 ttgaacaata ccaaaaagac cacggtccct aaacggttca tggtcttggt aatactgaga
     6601 ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt cttcccgatc tttgcctttg
     6661 ttttcatctg gactcacaga tagaatcttg ttgacccggt cttcaccaat ttcttctatg
     6721 ttggaatcat caaattgcag tagtaaccaa ggttgttgga cttgaatcga aaacaaacta
     6781 tcccgaatca aatccacact atcttttcct tggctttctg aattgggtac aacgatctcg
     6841 gttccaaggg taagggccgc ttcactaaca tctgagctaa agtcattgat ttttgtgata
     6901 taggtaggcg cataagcaat gaacgaaccc gatagtagaa aaatcatcac aaaattaatc
     6961 acggctcgaa ctgctttcgt ggtttctcgt tttaataatc cggtatacgc cacatacacc
     7021 cccagagcta aaatcatcaa taatagaaac ccaaaataaa atccggaggt ctggagtcca
     7081 ttttcagtaa taccagccaa tgtttggata ttctttccaa tactttcagc cgtatccgaa
     7141 atgaaatcta atttgtaagc ctcttgaacc acataacccg tggcgttcga caaatacaaa
     7201 ctcaccgtcc aaatgaaatt agtaatggca taaagaccat acatcacgct tttccctaag
     7261 ccatcacccc aattccaagg caaccaatcc caagagctat ccacaaagaa atctagttga
     7321 tagttgttca gtgaatattt ggaatataga tttcctgctt caactgtatc atcaactagc
     7381 cctgtcgcct cggctaccgt accaactaaa ctcaatagaa ctatcagagt gagtatgcta
     7441 ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact ccattctctc
     7501 ttgaacaggt ggacgggtat caaaggcatg gaagagttct tcaaagactg gatgtacctg
     7561 aaccacgcct acccgaccat acaaatcctg catcaagcat tggccatttt ccaaatctcg
     7621 taggcgtttt tgattgcttt catcttctcg atccaagcca aagaattcca aggttttctt
     7681 gatttcatga atatcggtac tgcgaaacgc aaacttcaac ccgatattat tcttcaagcg
     7741 ttcatccagt aaatcatcac tattttgcgt gacaaagtaa accccggcat tcatagcccg
     7801 cccagcacga acgagcttat tcgataatgt tttcccttgc gctacttgaa gaaaactcca
     7861 cgcctcatct aagtctacaa ttttgaagat ttctcgatta ctatggataa agtccaaggc
     7921 aaaagtggaa atgacgatta gcatggaaac actaagcaat tccatggtgg tatattcttc
     7981 aaaagtggtt tctgcatctg gtaaaaccaa atccgctact tgaatgatat ttaactgcct
     8041 atctaaacta atcgactggg taatcgaacc atctgagaaa agcaaatggg caaaatcata
     8101 atcggtaaag gactcgatgt gatccgcgat attttccgca actggtgagc cttcttttcg
     8161 aagctcatta attacttgta ataatccccg ttgattgctt tgggtaaccg tccgaatggc
     8221 cttacgcaac actggaaact tctccccatc tcgactagaa attcctgtta aaaaggtcag
     8281 aatatcaatt gctagacttt cagaatcctt ctttttctcc ataatcacat aaggatcaag
     8341 taatccttga ttttttggct tactagttaa gttgagaata ttgatttcat ccgcaatttc
     8401 tggcaaggtt tctttccagc cacc
//
LOCUS       DACEMK010000105         8368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEMK010000105 DACEMK010000000
VERSION     DACEMK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.108"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            76..456
                     /locus_tag="IW715_002295"
     CDS             76..456
                     /locus_tag="IW715_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1022882.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            438..1352
                     /locus_tag="IW715_002296"
     CDS             438..1352
                     /locus_tag="IW715_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1022883.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            1592..2281
                     /locus_tag="IW715_002297"
     CDS             1592..2281
                     /locus_tag="IW715_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022884.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2401..2487)
                     /locus_tag="IW715_002298"
     CDS             complement(2401..2487)
                     /locus_tag="IW715_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1022885.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            3133..4065
                     /locus_tag="IW715_002299"
     CDS             3133..4065
                     /locus_tag="IW715_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1022886.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            4082..4552
                     /locus_tag="IW715_002300"
     CDS             4082..4552
                     /locus_tag="IW715_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1022887.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            4636..4953
                     /locus_tag="IW715_002301"
     CDS             4636..4953
                     /locus_tag="IW715_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022888.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRI
                     LLF"
     gene            5243..6424
                     /locus_tag="IW715_002302"
     CDS             5243..6424
                     /locus_tag="IW715_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1022889.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            complement(6653..7801)
                     /gene="tnpB"
                     /locus_tag="IW715_002303"
     CDS             complement(6653..7801)
                     /gene="tnpB"
                     /locus_tag="IW715_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1022890.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(7818..8222)
                     /locus_tag="IW715_002304"
     CDS             complement(7818..8222)
                     /locus_tag="IW715_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1022891.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT         2958 a         1388 c         1394 g         2628 t
ORIGIN      
        1 tactcattgt gcctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact
       61 gaaagggtgg gacttatgag cgaacaaaat caaaatttgg ctagcgatca ttctaaaaaa
      121 tatacctatc gatccaagcc aaaacaaatc agttttcgag tgagcgaatc tgaatttgca
      181 aagctgaagc agtcagctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag
      241 gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgccca ggcaatcgcg
      301 cgcgatttag ccaaagctgg agggaatatc aatcaaattg ccaagtggtg caatacacac
      361 caacatgacg ttgctcctgg agacgcccaa cgtttatccg aaaacctaaa aatcatgcaa
      421 aaggagctac aaaagatatg gcaacagtta aagtaagtcg aaccacttcc tgtagccgag
      481 caatcaatta cgcagaacca cgagcaacca ttaaaacagg aataaactgt gacgtcgatt
      541 atgccaaaag tgaaatgaaa caaattcgca tgctgtatgg aaaagacgac cgtgtacagg
      601 cacacttatt gattcaatct tttcgaccag gagaaattac cgctgaaaaa gctaatcaac
      661 taggggcaga gtatgcaaaa aaaatagccc ccgatcacca aacagctatt tacacacata
      721 cagacaaaga ccatattcat aatcacattg tcattaactc cgttaatctg gagactggaa
      781 aaaaatttca agctcatggt caagcatttc taaacaaatg ttatgaccta aatgacgaga
      841 tttgtcttgc acataactta ccaatcactg agcgaggaaa gaaaccagaa aaacgaacca
      901 tgtctgaaat aaaattaaaa gaaaaaaatg aacctgtttg gaaagacgaa attcggattg
      961 cgatcgatca aacaatgaaa aacaaaaaaa ccagaaccta tgaccaattt tgtaattccc
     1021 tcaaaacatt ggggattcat tgcttcaatc gcgggaaaaa tttcacttac gaattgatcc
     1081 acaaaaagaa aaaagtacga tccaacagac taggaaagga ctacgaaaag gagacgattt
     1141 tacatgagct ggacagacga gaacgaacct ataaccaaca gcgaaccaac gagctacgaa
     1201 gagctcgagc tagcattcgc gaaactgcag aagaagtacg aagagaacaa cgaactctta
     1261 cgcctgaaca accggaaatt agaccggctc gaagaaacca tgaacaacac gttgaaagag
     1321 gaacagacca ccaacaagaa ttatcgctct aaaacgttaa aagacataga aaacgaaata
     1381 aagaaacaac gtgcttttct cgttccttat acgcaagaaa ggatacaaac tacgattgat
     1441 ttacaagtta aaaaactcca tcaagagcta ttagaccagc gaaataagga acagaaaaag
     1501 gtgcttattc aacgatcatt ggattttctt gcccccttat gttgcttagt cctgacactg
     1561 ttcgcatttt ggttagtatg gggaggctaa aatggcattt tatttaccct atctactcat
     1621 ttttgttagt atttcaggaa gtatttggct catctataaa atttttcaaa caagatattc
     1681 attaaaaggc tctaaaattc gatttaagcg atttttcttt cttggctgta tattttcctt
     1741 gataatcgtt tcaagcggct tattcggcgt tttagaggga aataagcgcg tttctagaag
     1801 tattttatta ggaaatgtca ctcaaaagta tgaatcagcg cgtgataaaa agaaaaaaga
     1861 gcaagcatta gcacaaaaaa tggaagagtt taccacttgt tatgaggaaa tgaacgatat
     1921 ttttgtgaat caagaaaaac gtttgacgga taaaaacatg gaaaaactca ctcgtcttta
     1981 tcaaaacttg ccagaaaaac agcaaaaaga ggttcaagac aactatgagc gaaccaaaaa
     2041 agacgtgcaa tatgtgaaag atacgaaagt ggaagaagct tgtagtgatt tatttggaga
     2101 tacaattcct ttttcaacta gcgaacaaga acgaaaagaa cgacaacaat cagtcacata
     2161 tgaacgctat gaaatcttgc ttcaacaagc aattaacata caaaatctga ctaaaaaaga
     2221 aacagcttta aattatttaa aaagcgtcaa ggaatggtta gatcagcaac agcaaaattg
     2281 aatttgattg aaattcatgt taaactaaac atgcaaatgt tcaaacatta gatacacgaa
     2341 agccccagag aggtcggatt ctctggggct ttttttaggt tgatgtcgcc tttactgatt
     2401 ttacttcttg tttcggttat tcaaccaata actgaataca gagatacaaa ctcctacaag
     2461 aagggggcaa ataaacagtt caaacatcag atacacctcc ttccagtcgc aagactgtcg
     2521 gataggcgac aagttattat accctacaaa attaaatctg tgtggcaatt ttaagtatct
     2581 cttatttccg ggttgttagg gcttgccctg accgcctgta gggcgcttta aattccagat
     2641 aaagtttttt atactaagac tgtcttaaaa cggcttagac aagctcttag agcctcaacc
     2701 ctcctttttg aacaatggtt ttagcaaagg agctggaggc gactgcgcag gtataatgtg
     2761 agagcgtagc gatcacacct ttttggcgac cgtagggagc gaggttttga attagcagtt
     2821 tagaaaagtt cgcgaacctg tttaaagaaa aaataattgt ttttttaggt gggaaatttt
     2881 gtctccctct tttataatct ttttttaaac ctctttttaa acctctttaa cgtcggctgt
     2941 gtccttactc ccccaaggta tacaaaattt tataggcatg aaattatctg tttataggca
     3001 tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat tatctgtttt
     3061 aggtattgtt tttagacata atatgtccta aaatgatgtc aataaaatca tttagactgg
     3121 agtaacaact aaatggcaaa tgaaatcgta aagtatgaac cagaattaaa taccattcct
     3181 ttaagaaaat ttaccccaat agaaatgaac ttgttttttt ctattgtctc tagaatgcgc
     3241 gataaaggta atcaaaaaat acgcttttat ttcagccaac ttaaagattt aagtgcgtat
     3301 aagcagactg caggagaagt ctttataaaa gatttggcaa gaacatacga tcatttgatg
     3361 gatttacgtt tcggaagaaa aagcaatggt ggattaacaa tagaacgatt tacaatgttt
     3421 actgattttg aaattcatgg agaagtcgat aacccttatg tagatataaa agtccacgaa
     3481 aaagctttgc ctttacttaa taatctagaa agctgggtac gtttctcttt aacagaattt
     3541 cgagatttaa atagcagtta tgctaaaaca atgtttagat tgctaaaaca ataccgaact
     3601 caaggatggg ctgaattttc taaggaagat tttttagagc tattagatat tcctaaaagc
     3661 tatagacaaa caaatataaa tcagtttgta ctcaaaccaa taaaagaaga actaactcca
     3721 ctatttaaag gattaactat tcgtaaaaaa tatggtaaag gtcgaggtaa acctgttatt
     3781 ggttatcgtt ttacttggaa agctgaaatc aaccatgctg acgatttttc aaaagggaag
     3841 caagaagatt tacgtataaa attatttaat attgaacaca atggagaatt aacacaaaaa
     3901 gaaaaatggc gtgctaaaga tcgaatacta aatcttccgt taggtactca tgaagctgat
     3961 tttaaaaaac aacaacaaac cgaaaaagaa gaagcggaaa aacaatcaat ctcaaatgag
     4021 cttaaacaag atttattaga aaacttacaa aacttattcg attgaagcat aaggagtatt
     4081 tatgatagat aaaacgatca aagaactcgc ggaagaattt catgtttcaa aacaagcaat
     4141 taggaaaagg ctatcagctg attttagaga aaactatgtg caaactgtta ctaggaacgg
     4201 agtgaaaacc ttagttgtca gctacaaagg atatttgctt ttaaaacaac attttttagg
     4261 tagtaacacc actggtaacc aagtagaaac cgttgctagt aacgatgaaa actcgattat
     4321 tgttttttta catgaacagc tccaagaaaa agatactcaa ataaaacaaa tgcaaaagtt
     4381 actcgaccag caacaacaac tcaccttgca ggcaaatcaa cagatcgaaa aactgcaaga
     4441 acagttacaa ttagcttata cggaagaacc tgaagaagag gaattaactt ctgtcaataa
     4501 agaaaacaac aatgcaataa caacagaaaa aaagtggtgg aaattttggt aacaattacc
     4561 aactttccat aaagaagaaa tcaatttcta tagactctat ctttctaaga ataaaaaata
     4621 tgtttaggtg atcaaatgac tataagtaaa gtaatcaaac aaaaaagaat agaaaaacaa
     4681 ctaactcaag aagatttagc tgaaatgctt ttagtatcta aaaaaaccat ttctaattgg
     4741 gaaaatggtc gaacaatacc agatacagaa aatttaattc agtttgctaa gttatttgat
     4801 ttatcactag acaatatttt gttaggagaa gaaaaaatta tggtaaaaaa ttatggtaaa
     4861 aaatcataca aaaacactaa tactattatt tcattttgta atttttttaa gtttagtttt
     4921 caccttaatt cagtccggat tcttttattt tagccattca attgccactt ggttattaat
     4981 attttttcta agtttactat taataattaa tacattaata attaatactt taattattat
     5041 ctttaataaa catagaaact tatttcttca cagcatattt ccaattgttt tattaatttt
     5101 tctatacttt atataaaaag atagaactat ttagtaagat aaaaaagtag ctaattgtaa
     5161 ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt gtgttatttt tatttataaa
     5221 ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc tacaaaaatc agttgaatta
     5281 tgggctttta ttgccagttt ttctgctatg tatcattgga ttactttctt tatatgttgc
     5341 tttatatcac gactcttaca ctaattcaaa aatcacaaaa gaattaatta aacaagtatt
     5401 gtggtatagt attgggttca cttcagcaat aactatttat ttttttaatc ctaaaatttt
     5461 gtggaaatta actcctatat tttacggatt atgcatcata atgttattac ttctcttgaa
     5521 attctataat ccacaattag cagtcttaac tggagcaaaa aattggtttt atattgctgg
     5581 tttttcgttt caacctactg agttagtcaa aataggatac acccttatgc tagctaaaat
     5641 aaccgtcaat tatcagcaaa aatctattac tagtaatacg aaagatgatt ggatttattt
     5701 acttaaaact tttattgcta ctcttccaat aggaatatta ttattttttc agaacgatct
     5761 aggaacaatg ttagtcttca ttgttattta tatttttatt ttgtttacgt ctaacataaa
     5821 tataaaaata attgccccaa taatcgccat tggtgttgtt ttatccttaa tatttttata
     5881 tttggttata tcagatacag gaagagattt cttattaaaa ataggatttc acgactatca
     5941 gtttaaaaga atagactcat ggttgaatcc atttttagat ccaaatggat ctagctatca
     6001 gttagcaaat agtttaattg ctattggctc tggcggactt ttaggtactg gttttaatgt
     6061 atcaaacatt cacgttcctg ttagagaatc cgatatgata ttttctgtaa ttggagaaaa
     6121 ttttggcttt ataggaagtt gtcttgtaat attcctatac ttttatttaa tttatcaaat
     6181 gtataaaaca tgttttaaaa gcaataactt atttttcacc tatatcggta taggcattgt
     6241 tgcaatgatt ttttttcatg tttttgaaaa tataggtgca agtatcggat tactacctct
     6301 aactggtatt ccactcccct atattagtca aggtggttct gccattctta gtaatttttt
     6361 ggggttggga tttatgttgt gttgtagtag atatactaac gaaaaaaaag gaacacaaaa
     6421 atgaattcaa aataacactt tatgaataga atgtctattt caataaaaat tatgatacct
     6481 taaagttaat cagtgaacta ctcaccactt agctaaacct gacggttctt aacgcttgaa
     6541 gtgggagctt cttgggaata gagcatactt gatagcgtat ttctttacta acagcggtgt
     6601 ctcttattta ccaagctatc cccgtagttc ctacggttct tgatgattgg atctaagcca
     6661 atcccataga atgtagtctt agaccttcgg tcagaatatt gatactagca ttgatatcac
     6721 gatcatgatg agtatgacaa acaggacaag tccattctcg aatttcaaga gatttcttgc
     6781 catctttatg tccgcactct gaacaaatct gactagacgg aaaccattta tctactttga
     6841 tgatttttcg tccataccag tcagccttgt attgtaattt agtcacaaac tttgaccaag
     6901 aaacatcaga aatactttta gctaatttat gatttcgcaa catacctttt gtgtttaagt
     6961 cttcaataca gataatatcg tgatttttga tcatttctgt actcaactta ttcagaaaat
     7021 cagtacgttg attcattact ttttcatgca atctagctac tttgcgtttt tgtttttgat
     7081 aatttttagc ttcaaataga ttggtacctt tatttttagc caataaagca cgcctagaca
     7141 atttgcgttg ttcacgcttt agtttctttt ccattttgga cgtgaattta ttattatcaa
     7201 ttttttgtcc atcagaaaga atcgcaaagt ccataatacc taagtcaata ccaattgtgg
     7261 aattagtttt aggtaattcg ataatttctt ctttacacaa caaagaaata tagtatttac
     7321 cgctagaatg acgtgatatt gtagcggatt tgataatccc ttttggttgt ctatgaagct
     7381 taattcttac taatgacttc aatttaggaa ctttgatgaa tttactatca atcaaagcaa
     7441 ctgtaccatt ttgattattc gttgtataac tctgaacagg atttttctta cttttgaaac
     7501 gtggaaatcc aacggattta tcccgaaaaa aattcttgta tgctttatct aaattaagtt
     7561 gggcattggc taaagcaagg ctatcaattt ctttcaaaaa cggaaattct ttcttgtatt
     7621 tagctggtgt cggaaaagtc atttttttag aagaatcatt tttaacttct tcatacgcct
     7681 tttttcggtc atcaagcatt agattgtaga ccttacggac acaaccaaaa gatttggcaa
     7741 agaagatttc ttgttcctct gttggataga ttctgaattt gtatgctttt agttgttcca
     7801 taaagttcac ctactttcta tttatgattg tttccttgat tttggatata cttcttaatc
     7861 acatcaattg gtgcgccacc agttgtcaga agacaaaaac ttttagacca gaacatttct
     7921 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
     7981 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
     8041 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
     8101 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
     8161 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
     8221 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa ataaaaaggc
     8281 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
     8341 catcacccac ctatagagga tgggcgac
//
LOCUS       DACEMK010000106         8287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEMK010000106 DACEMK010000000
VERSION     DACEMK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.109"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            213..1406
                     /locus_tag="IW715_002305"
     CDS             213..1406
                     /locus_tag="IW715_002305"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1022892.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1536..3008
                     /locus_tag="IW715_002306"
     CDS             1536..3008
                     /locus_tag="IW715_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1022893.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3091..3879
                     /locus_tag="IW715_002307"
     CDS             3091..3879
                     /locus_tag="IW715_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1022894.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3946..4614)
                     /locus_tag="IW715_002308"
     CDS             complement(3946..4614)
                     /locus_tag="IW715_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1022895.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4729..6441
                     /locus_tag="IW715_002309"
     CDS             4729..6441
                     /locus_tag="IW715_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022896.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6444..8216
                     /locus_tag="IW715_002310"
     CDS             6444..8216
                     /locus_tag="IW715_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1022897.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2539 a         1387 c         1822 g         2539 t
ORIGIN      
        1 aaaagcttag cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa
       61 tgtaaaatat ttttatattt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
      121 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
      181 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
      241 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
      301 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
      361 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
      421 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
      481 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
      541 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
      601 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
      661 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
      721 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
      781 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
      841 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
      901 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
      961 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     1021 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     1081 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     1141 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     1201 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     1261 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     1321 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     1381 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     1441 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     1501 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     1561 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     1621 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     1681 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     1741 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     1801 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     1861 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     1921 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     1981 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     2041 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     2101 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     2161 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     2221 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     2281 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     2341 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     2401 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     2461 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     2521 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     2581 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     2641 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     2701 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     2761 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     2821 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     2881 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     2941 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     3001 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     3061 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     3121 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     3181 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     3241 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     3301 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     3361 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     3421 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     3481 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     3541 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     3601 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     3661 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     3721 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     3781 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     3841 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     3901 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     3961 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     4021 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     4081 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     4141 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     4201 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     4261 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     4321 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     4381 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     4441 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     4501 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     4561 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     4621 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     4681 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     4741 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     4801 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     4861 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     4921 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     4981 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     5041 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     5101 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     5161 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     5221 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     5281 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     5341 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     5401 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     5461 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     5521 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     5581 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     5641 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     5701 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     5761 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     5821 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     5881 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     5941 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     6001 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     6061 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     6121 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     6181 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     6241 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     6301 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     6361 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     6421 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     6481 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     6541 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     6601 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     6661 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     6721 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     6781 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     6841 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     6901 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     6961 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     7021 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     7081 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     7141 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     7201 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     7261 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     7321 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     7381 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     7441 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     7501 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     7561 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     7621 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     7681 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     7741 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     7801 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     7861 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     7921 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     7981 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     8041 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     8101 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     8161 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     8221 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     8281 ggcagaa
//
LOCUS       DACEMK010000107         7761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEMK010000107 DACEMK010000000
VERSION     DACEMK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.112"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1094
                     /locus_tag="IW715_002311"
     CDS             147..1094
                     /locus_tag="IW715_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1022898.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="IW715_002312"
     CDS             1106..2026
                     /locus_tag="IW715_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1022899.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="IW715_002313"
     CDS             2067..3524
                     /locus_tag="IW715_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1022900.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="IW715_002314"
     CDS             3869..4483
                     /locus_tag="IW715_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1022901.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="IW715_002315"
     CDS             4476..6200
                     /locus_tag="IW715_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1022902.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="IW715_002316"
     CDS             6197..7648
                     /locus_tag="IW715_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1022903.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1291 c         1543 g         2309 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DACEMK010000108         7624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEMK010000108 DACEMK010000000
VERSION     DACEMK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.113"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(15..707)
                     /locus_tag="IW715_002317"
     CDS             complement(15..707)
                     /locus_tag="IW715_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1022904.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            792..968
                     /locus_tag="IW715_002318"
                     /pseudo
     CDS             792..968
                     /locus_tag="IW715_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="IW715_002319"
     CDS             complement(1144..1713)
                     /locus_tag="IW715_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1022905.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="IW715_002320"
     CDS             complement(1787..2110)
                     /locus_tag="IW715_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022906.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="IW715_002321"
     CDS             complement(2264..3076)
                     /locus_tag="IW715_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022907.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="IW715_002322"
     CDS             3430..4278
                     /locus_tag="IW715_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1022908.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="IW715_002323"
     CDS             4423..5364
                     /locus_tag="IW715_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1022909.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="IW715_002324"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="IW715_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1372 g         2469 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacctatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caacgtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtgtgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg taggatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcggcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttc gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 acttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gttacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgagaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatga atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagctttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaacg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctcgaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caac
//
LOCUS       DACEMK010000109         7596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEMK010000109 DACEMK010000000
VERSION     DACEMK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.114"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(135..425)
                     /locus_tag="IW715_002325"
     CDS             complement(135..425)
                     /locus_tag="IW715_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022910.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IW715_002326"
     CDS             complement(543..803)
                     /locus_tag="IW715_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022911.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IW715_002327"
     CDS             complement(940..1353)
                     /locus_tag="IW715_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1022912.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IW715_002328"
     CDS             complement(1809..2294)
                     /locus_tag="IW715_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022913.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="IW715_002329"
     CDS             complement(2433..2678)
                     /locus_tag="IW715_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1022914.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="IW715_002330"
     CDS             2871..3671
                     /locus_tag="IW715_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1022915.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="IW715_002331"
     CDS             3690..5558
                     /locus_tag="IW715_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1022916.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="IW715_002332"
     CDS             5551..6042
                     /locus_tag="IW715_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1022917.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GIRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="IW715_002333"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="IW715_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1022918.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..>7596
                     /locus_tag="IW715_002334"
     CDS             6602..>7596
                     /locus_tag="IW715_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1022919.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1442 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagaag ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtatacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACEMK010000110         7387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEMK010000110 DACEMK010000000
VERSION     DACEMK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.115"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            362..805
                     /gene="rplM"
                     /locus_tag="IW715_002335"
     CDS             362..805
                     /gene="rplM"
                     /locus_tag="IW715_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1022920.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            821..1213
                     /gene="rpsI"
                     /locus_tag="IW715_002336"
     CDS             821..1213
                     /gene="rpsI"
                     /locus_tag="IW715_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1022921.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1302..2468)
                     /locus_tag="IW715_002337"
     CDS             complement(1302..2468)
                     /locus_tag="IW715_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1022922.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2523..3173)
                     /locus_tag="IW715_002338"
     CDS             complement(2523..3173)
                     /locus_tag="IW715_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1022923.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            3248..3451
                     /locus_tag="IW715_002339"
     CDS             3248..3451
                     /locus_tag="IW715_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1022924.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            3459..3785
                     /locus_tag="IW715_002340"
     CDS             3459..3785
                     /locus_tag="IW715_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022925.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            3940..4983
                     /locus_tag="IW715_002341"
     CDS             3940..4983
                     /locus_tag="IW715_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1022926.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            4985..5203
                     /locus_tag="IW715_002342"
     CDS             4985..5203
                     /locus_tag="IW715_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022927.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            5190..>7387
                     /locus_tag="IW715_002343"
     CDS             5190..>7387
                     /locus_tag="IW715_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022928.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
BASE COUNT         2607 a         1102 c         1380 g         2298 t
ORIGIN      
        1 agcctgccgt tcagctccga gtcacaagaa acaattttaa aaaacagctt cttatgtttt
       61 atggatattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcaaaataag
      121 gggttgtgac aagacttttg tcacagcctc tttttgtttt tcattttgac aggaaacgac
      181 ccttttatat aaaagaaaga aaagaagtaa agtttcatat aaatttttca atcaagcaga
      241 aaaacgttga cattgactag attagcgggt aatatgttaa atggtattgt ttgccccaca
      301 aagagccccg gaaactcgaa gtgtatgtca aacatcagaa cttaaattgg aggaaaataa
      361 cgtgcgtaca acatatatgg ccaaaccagg cgaagtagaa cgtaaatggt atgtagtaga
      421 cgcaactgat gttccattgg gacgtctttc aacagttgtt gcatctgtac tacgtggtaa
      481 aaataaacca actttcacac ctcatttgga tactggcgat ttcgtcatcg tcatcaatgc
      541 agacaaagtg aaattaacag gtaaaaaagc gactgacaaa atttattacc gtcacagcaa
      601 ctacccagga ggattgaaat caatcactgc tggcgaattg cgtgctaaaa attctcgtcg
      661 tttgatcgaa acttctgtaa aaggtatgct tcctaaaaac actttaggcc gcaaacaatt
      721 tacaaaattg aacgtatacg gtggagcaga acatccacat gcagcacaac aaccagaagt
      781 tttagacatc acaaacttaa tctaatagga gggaatttca ttggcacaag ttcaatatat
      841 cggcacaggc cgtcgtaaaa acgcagttgc tcgcgtacgt ctagtaccag gaactggcaa
      901 aattactgta aataaaaaag atgttgaaga atacattcca catgctgact tgcgtgaagt
      961 aatcaatcaa cctttcgctg ttactgaaac aaaaggcgca tatgacgtat ttgttaacgt
     1021 aaacggcgga ggatacgctg gacaatcagg agctatccgt catggtatcg ctcgtgcatt
     1081 gctagaagtt gatcctgact tccgtttagc tcttaaacgt gctggcttac ttactcgtga
     1141 cgcacgtatg gtcgaacgta aaaaaccagg tcttaagaaa gcacgtaaag cttcacagtt
     1201 ttcaaaacgt taatcgttgt tataccaacg atttcagaca ctttctcatt gcgagaaggt
     1261 gtcttttttt cgttctggta atgagaaagg taatgacaaa attataaacc agtgtaagca
     1321 atgaattttt ctaacgcatt tcgttgagct ttttcagtaa catgagaata agtgttaagg
     1381 gtagtttcag catttttatg acccaatcga tcttgtgttt ctttgatact tgctccacct
     1441 tctagtagaa gagtagcgtg agtgtgccgg aatccatgaa tagtaacgtg cttaaattta
     1501 tatttcttct ctaaacgttt caatatatta tttgaatagt ccgcatgcaa aggttgatta
     1561 atgtcaccgt ctgcatttac ataggtaaag ataagttgat tttttgtatt tgtatttatg
     1621 ccaaactgca aaagctctcg agcttgttct tttttccaga tttttaacat ttcaatagtt
     1681 tgtgcatcaa ttggtaatat tcgttcggaa gatatatttt ttgtattttt ttgaatcctg
     1741 acagtttttc ttcttgcatt cttctcttca tttcgaatag ttgcatagct ctttctaaca
     1801 tacattagct gttcttcaaa ataaatgtca gaccaatgaa gagcagctag ctctccttta
     1861 cgtataccag tagtaatcaa aagctggaag attaaaatat gttttaaagg ctcttcttta
     1921 ttgagaatat cccaaaaagc ttttagttct tctaatgaaa agtaattttc attttctttc
     1981 ttttcccgct ttggtagatt tgtatttgcc ataggatttc ggtgtaatag ctctgcaaaa
     2041 atggcatact caaatatttg agagctgtaa gacttaagct gtttgatatt cttgaatttt
     2101 aaagcccatt cattgactgc attctgacag tgaacaggag taatcttaga aatggactta
     2161 ttcccgaatt ttggtagtat atgcaggcgg aaaaggtctt ttgtacgttg atatgtcgtt
     2221 tcttgtacac gattcctata agaaggcaac cattcttcat acactgttat aaacaagtca
     2281 ttagaaggct tatgatagcc ggtatcttca atctcgacct tttttcttgc ttcgtgtaac
     2341 ttggcttctt tttcagtttt aaaccctttt tttacatagt attcttgctt tccattttca
     2401 tctttacgaa catacaagcg gacactatat cgtgcaccgc tttttgttgt gtacttttgt
     2461 attgacataa ctaatctctc caatctgata acttggcgag ttggctgatt gaataattag
     2521 ttttattgaa gttttgatat taatcgagct gttatctctc tttttagatt gttaacctgt
     2581 acttgggtta agttgccaaa taactcagat tctaactctt ttctttcatc gatatttaag
     2641 tttaaagctt tattaataaa cttcttttgt ttggcaaata gaatcgtgta ttcaaaggag
     2701 tcctctagaa tactttttaa aaaaagaaag tcggagtcat cttcttgaag tttgacttca
     2761 agtaaagaag tcatggaaaa gtatagatta gataattcag taaaataatc tgagtcaaga
     2821 ctggcaatat tcttgactat tttgtccttt ttttctaaac gagcagtttt tattctatca
     2881 agattaggtt gttctatgga atttgtttcg tctaatccca tcaaataact tacaggaaca
     2941 tggaaataat cagcaaggat attccatgtt tcagtatttc taggatttcg ctcaccacgg
     3001 taatagttcg taagagttgt atagttaata ccagtttctg tactaatttg tttaatggtc
     3061 tttttactgg aatcaatata aaattttatt ctattcaaag taacaccccc tatagtaaat
     3121 tccaaactat tatagcataa aaaccacaaa ttgagtaatg ttttaattga cattactcgt
     3181 ttcgtggttt aaaatgtgct tattaagata aaaaccacaa agtgaataat ttaaaaggag
     3241 gcgctgaatg ataactctta atgatcaatt cattagaagc ttaagaagac atagagccga
     3301 tcttatactt actaaaaacg atgcagcaaa attgataggt ataaatcgga aaacatatgt
     3361 caaaatcgaa aatgggagca aagaaagtat tagagcatct acttatcaaa agttggtaaa
     3421 ttggttactt aatgatttga aatctaaata aatacaactt ggcgagttgg ctgattgaag
     3481 aaatagaaaa tgagaggaga aaaataatgg atgcaggaat tacagtaatg ttagataaac
     3541 aacaaacgaa tcaattgaaa aattatgttt ttgaaatgac aaaagaggct attgatcaag
     3601 caagaattga taccgggtta gaaagaccat ttctaaaagg aaaagagatg gctaaatatt
     3661 tgaatgtatc atatacaact tttttgaaat ttaaaagaat gggattgcca gttattttat
     3721 tagaaaaaat ggagttgttt tcaaaagaag aatgcaaaaa atggatattg agtcatcaaa
     3781 tttaaaaaac gaggtgtttt tttgaataat gatgaagtag aaaaaattaa aaagagtgtt
     3841 cccgtcagtt tgcagatcgg gggaacactc aataataaag ttctgagaaa actttatttg
     3901 aatctattat agcaaataaa atcagaagca ggtgtgacaa tgaatgaaaa agaagttgaa
     3961 agcttagttg aaaagcaagg ctattctaat tctgatatat atgcagaatt atactatcaa
     4021 tatcctattt ttttaaacgt aaatgaaaaa tataagaaaa tgaaactaag ttctaagtta
     4081 gtttatatgt tactaaaaga tagaaataga ctttcattga aaaataactt gtatgatgag
     4141 aatcaaaaaa tttttttgat atttgaaatc aaaaatctcg tagaaatttc gggaatgtca
     4201 aaaaatacaa tcttatcagc actagatgaa cttcaaaaag ttaaacttct tgtgaaaaga
     4261 aaaaatggct tcaataaaaa taaaatgcaa atgttcccaa atttttatta tttgttaaag
     4321 cctgtactgt gcaaagatga tgtttataaa attaataaga taacccatca agaaaatagt
     4381 caaaaaaact tgatagttca aaatttgaac tatggaacaa atcaatattc tgttgatagt
     4441 tcaaaatttg aactatgcga cacatcaatg aaaacaagag acaaaaacaa ttttgctgat
     4501 agttcaaaaa atgaactaga attatataag tcttataaag atagtaaaga tattaaagaa
     4561 tcacataatg atgatctttt tgaatcactt tcaactaaaa ataataaatt agatgaagaa
     4621 ttgatcgaaa catttattga agaaaatctt cttaaacaaa tgtggggaga aagtattgtg
     4681 aattgcttaa aactggctag caatagtgat tatcgtcaat tcgacaactg gtacaaaaaa
     4741 ttcaattatg caattaaatc tgctgaaaaa gaacaaaaag ttcaactgaa aattatctat
     4801 gaaatttgta ataatagtta ttttgtagat catgttagag aacagctagc aatgacttta
     4861 cgtgacttga tacgaagagc aaaaacagat catcgtatca aacaaaaaaa taattatatc
     4921 tttacttctc taaaaaacaa agcgttagaa ctaatcaagc tacaaaaaaa agagggaatt
     4981 taaaatgatt gaaaatcatc tagtagataa gattcactgt cttgatcaaa acgcagatac
     5041 tcttgagtta accgagagta tctgctcaaa tactattgtt ctcgatttaa aagctgttaa
     5101 tgatgattac gtaactcata tcataattga cagaaaaata gctcagaaac tctctgagag
     5161 cctaagaaaa tgggctgagg gaggtaacaa tgaaaataat taaaaaaaag attgctaata
     5221 gaaaaacaaa ttggttaccg gttatttttc ttgttctaat agctgtgatt ctttttacga
     5281 gtaacattgc agaaggcagt attttcaatt ggtttgatga tactgagagt caaactgagt
     5341 ttttaactaa tccgctttac cagaattatt tgaaaaaata caacggaaat ttttacagcc
     5401 agttcggtat gtggataggc tggtctgtta taaaatcaat gttcgctgtt gtcgatggca
     5461 ttcaaaatat gattcccgaa gttttagatt tgtttagctt tgtagaaagt accggattga
     5521 acaaagtcta tcaagctgtt ttaagtacca ttgttgttgg cttaatgata ctttcactag
     5581 tgattattgg ttataaaatg attacaggta agggaacggt tgatctaaaa tctgttggaa
     5641 tgaatgttgt aatgtcaatt tcattgattc ttttaatgcc aacgttgatt cacagcggaa
     5701 tagagttttc taaaacattt tataatgata cgactacgat tacaaattcg gacgaaggcg
     5761 tggcgtggtc gctgattgaa caaggggtta ctgatttagc ttatatcaat aaaacagatc
     5821 aatattccat gatcgataaa acgaatgacc ggaacaatct aacaaaagaa aatttttgga
     5881 aaacggatct tacgcaagta ttgacagata atgtactcga caaaatggaa aaagagaacc
     5941 caatagctga taatctgcgt tatgaattag ttgaaaattc tgataatgaa tttgttgcta
     6001 ccaaattttc ggataatttt ctgtcgacct ttgttgaggg cattaaatca ggatattatc
     6061 gctatcaagc aaatctatgg gggatttctt ttggattgat tgctttagcg gtagcgtatg
     6121 tttttagtgc tttcattata attacagcaa tactcgagct agcgtttaaa cgcgtacttg
     6181 gagttttagt atttgctact gacttggaaa caggccaacg atcaaagcaa gttttgtcag
     6241 atattttaca gtgttatttg acagtcggat ttcaagggtt cggattgtct atgtttgcaa
     6301 tgtttgttaa ttatctcgga acaggtagcg gttcttctgt aaatcctttt ataaaagcag
     6361 tagcgtacgt ctgcgctgtt attgtgctga taaagggtag tggaacagtc atgcgttact
     6421 ttggcgtgga tattggttta aaagaaggct atggccagct tgcttcagca tttggcatgg
     6481 gagctatgct gtttcgtaaa ggaacaggtg gtatgaaccg atctaaaggt gggaaaaata
     6541 gtggtggaaa cgatcaagcg gaaggggaag aacgaaaacc tgaacgcaac tttggtcaat
     6601 cactttctca aaaagctaat aaaacgggga aaacacttgg gtatgctcgt gaacgaggat
     6661 tatcaggctt agcttcggat ggagctacaa tggccagtga aagagcaaca aaaccattta
     6721 agagtatgcg tgatatggct aatgatacca agaataaatt caaagaaggt ttagatgatg
     6781 gtaccgtgta cgctattaat aaaaatagca aacctatagt agctaaaaac aaagaagatg
     6841 aaactggaaa atatgctgat tctatgccaa cacgtctttc ggatagaaaa gatggcgcta
     6901 aagttgaaaa cgctgataga attatgtcta gttctgaacg tatgcgtgaa gcaatgaaaa
     6961 ataatgctga atcaaatatt aatcctgcaa gtgcaattca gcaaaaagtt aaacaagata
     7021 ttgaagaacg taaaaatgct atgcacggtc aagctaagtc tgctgaagta ttaattaatc
     7081 agaagcgaca ggaagcaaaa tacacgcctg aagcaatgaa tcgtgaggaa atgttaagga
     7141 aacgtgtaga agggcgtaca ggcgctaata tggacgaaaa agaagcgttg actaaagaaa
     7201 gaatccaaga agctaaaaat tcaaataccg gattagaaaa atcagtcaaa gaaaatctac
     7261 aaaattctac aagtagtcaa gataggcgct cagtagatgt tcgagaaaac ttacaaaata
     7321 ctacaagtag tcaaaatgga aatcgaacag tatatgtacg agaaaatgta caaagtagtg
     7381 ggacaag
//
LOCUS       DACEMK010000111         7187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEMK010000111 DACEMK010000000
VERSION     DACEMK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.116"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW715_002344"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IW715_002344"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1022929.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IW715_002345"
     CDS             complement(1172..1867)
                     /locus_tag="IW715_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1022930.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IW715_002346"
     CDS             complement(1880..2596)
                     /locus_tag="IW715_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1022931.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IW715_002347"
     CDS             2926..3534
                     /locus_tag="IW715_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1022932.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IW715_002348"
     CDS             3859..4182
                     /locus_tag="IW715_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1022933.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IW715_002349"
     CDS             4364..5734
                     /locus_tag="IW715_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1022934.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IW715_002350"
     CDS             6081..>7187
                     /locus_tag="IW715_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1022935.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACEMK010000112         7153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEMK010000112 DACEMK010000000
VERSION     DACEMK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.117"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1368)
                     /locus_tag="IW715_002351"
     CDS             complement(join(<1..795,795..1368))
                     /locus_tag="IW715_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAQ1022936.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIK"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="IW715_002352"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="IW715_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1022937.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="IW715_002353"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="IW715_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2652..2882)
                     /locus_tag="IW715_002354"
     CDS             complement(2652..2882)
                     /locus_tag="IW715_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1022938.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3112..3930)
                     /locus_tag="IW715_002355"
     CDS             complement(3112..3930)
                     /locus_tag="IW715_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1022939.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="IW715_002356"
     CDS             4091..4780
                     /locus_tag="IW715_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1022940.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="IW715_002357"
     CDS             4794..6296
                     /locus_tag="IW715_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1022941.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="IW715_002358"
     CDS             6309..6785
                     /locus_tag="IW715_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1022942.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="IW715_002359"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="IW715_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2316 a         1131 c         1295 g         2411 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgt
//
LOCUS       DACEMK010000113         6974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEMK010000113 DACEMK010000000
VERSION     DACEMK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.118"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(362..1480)
                     /locus_tag="IW715_002360"
     CDS             complement(362..1480)
                     /locus_tag="IW715_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022943.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="IW715_002361"
     CDS             1951..3258
                     /locus_tag="IW715_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1022944.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="IW715_002362"
     CDS             3377..4576
                     /locus_tag="IW715_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1022945.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="IW715_002363"
     CDS             complement(4603..5871)
                     /locus_tag="IW715_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1022946.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="IW715_002364"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="IW715_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
BASE COUNT         2113 a         1457 c         1241 g         2163 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 ggcatctgcg gtaa
//
LOCUS       DACEMK010000114         6944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEMK010000114 DACEMK010000000
VERSION     DACEMK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.119"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW715_002365"
     CDS             <1..1023
                     /locus_tag="IW715_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1022947.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IW715_002366"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IW715_002366"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1022948.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IW715_002367"
     CDS             3389..3985
                     /locus_tag="IW715_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1022949.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IW715_002368"
     CDS             3998..4897
                     /locus_tag="IW715_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1022950.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IW715_002369"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IW715_002369"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1022951.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IW715_002370"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IW715_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1022952.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IW715_002371"
     CDS             5430..5663
                     /locus_tag="IW715_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022953.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IW715_002372"
     CDS             complement(5822..5944)
                     /locus_tag="IW715_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1022954.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IW715_002373"
     CDS             6134..6358
                     /locus_tag="IW715_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022955.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            6801..>6944
                     /locus_tag="IW715_002374"
     CDS             6801..>6944
                     /locus_tag="IW715_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1022956.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCG"
BASE COUNT         2470 a         1239 c         1470 g         1765 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaagggaaa tatctaagct acccacgaac agatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagctaa agacagtcgg
     2341 aaaagtacca gtagatctag gttggaaaaa gctgtttggc aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcgatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtcttgtcgc ttttcgttga
     6721 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct
     6781 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
     6841 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
     6901 aatgtatcaa aagctattgt ttgtatcaat gtccaacatg cggg
//
LOCUS       DACEMK010000115         6651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEMK010000115 DACEMK010000000
VERSION     DACEMK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.120"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            54..506
                     /locus_tag="IW715_002375"
     CDS             54..506
                     /locus_tag="IW715_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022957.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IW715_002376"
     CDS             520..1209
                     /locus_tag="IW715_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022958.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="IW715_002377"
     CDS             1334..3685
                     /locus_tag="IW715_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1022959.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="IW715_002378"
     CDS             3765..4160
                     /locus_tag="IW715_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022960.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="IW715_002379"
     CDS             4485..5102
                     /locus_tag="IW715_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1022961.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="IW715_002380"
     CDS             5143..5442
                     /locus_tag="IW715_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022962.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="IW715_002381"
     CDS             5476..5643
                     /locus_tag="IW715_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1022963.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6251)
                     /locus_tag="IW715_002382"
     CDS             complement(5688..6251)
                     /locus_tag="IW715_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1022964.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6279..>6651)
                     /locus_tag="IW715_002383"
     CDS             complement(6279..>6651)
                     /locus_tag="IW715_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1022965.1"
                     /translation="LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQS
                     VGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRR
                     NGTLFGFSVSTEIKVLMGITA"
BASE COUNT         2248 a          949 c         1329 g         2125 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttcggttct gttgcgaagt
     6241 ttgaaaatca aacgcgccct ctctgaactc caaatatctt aggctgttat tcccattaat
     6301 accttgattt cagtagacac cgaaaagccg aagagcgttc catttcttcg gttcttttta
     6361 tatattcctc gaatggtctc catgccctta atcgtggaag aggctgtacg gagactttga
     6421 taaaatttat ttcgtcgttt aataggtcga tggtcttgtt ctattaaatt gttaagatac
     6481 ttcacagttc ggtgctctgt cttagtatat aaacccacac tctgtaactt tctaaaggcg
     6541 gagccaagag aaggtgcttt atcggtcaca attgctttcg gctcaccaaa ctgtttatgg
     6601 agtcgtttta agaaagcata ggctgcttgc gtatcccgtt tctttcgtaa c
//
LOCUS       DACEMK010000116         6452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEMK010000116 DACEMK010000000
VERSION     DACEMK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.121"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..550)
                     /locus_tag="IW715_002384"
     CDS             complement(95..550)
                     /locus_tag="IW715_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022966.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="IW715_002385"
     CDS             complement(704..1912)
                     /locus_tag="IW715_002385"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1022967.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="IW715_002386"
     CDS             complement(2024..2356)
                     /locus_tag="IW715_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1022968.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="IW715_002387"
     CDS             2504..3379
                     /locus_tag="IW715_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1022969.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="IW715_002388"
     CDS             complement(3461..4042)
                     /locus_tag="IW715_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1022970.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="IW715_002389"
     CDS             complement(4048..5307)
                     /locus_tag="IW715_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1022971.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="IW715_002390"
     CDS             complement(5309..6355)
                     /locus_tag="IW715_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1022972.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1798 a         1334 c         1100 g         2220 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataaata aa
//
LOCUS       DACEMK010000117         6182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEMK010000117 DACEMK010000000
VERSION     DACEMK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.122"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW715_002391"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IW715_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1022973.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IW715_002392"
     CDS             complement(2203..4389)
                     /locus_tag="IW715_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1022974.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW715_002393"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IW715_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1022975.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IW715_002394"
     CDS             complement(4611..5051)
                     /locus_tag="IW715_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1022976.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IW715_002395"
     CDS             complement(5125..5664)
                     /locus_tag="IW715_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ1022977.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1603 a         1390 c         1090 g         2099 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccaacccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aa
//
LOCUS       DACEMK010000118         6124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEMK010000118 DACEMK010000000
VERSION     DACEMK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.123"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..189
                     /locus_tag="IW715_002396"
     CDS             <1..189
                     /locus_tag="IW715_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1022978.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="IW715_002397"
     CDS             complement(275..1369)
                     /locus_tag="IW715_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1022979.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1558..2319
                     /locus_tag="IW715_002398"
     CDS             1558..2319
                     /locus_tag="IW715_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1022980.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IW715_002399"
     CDS             complement(2434..2874)
                     /gene="fabZ"
                     /locus_tag="IW715_002399"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1022981.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3094..3849
                     /gene="fabI"
                     /locus_tag="IW715_002400"
     CDS             3094..3849
                     /gene="fabI"
                     /locus_tag="IW715_002400"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1022982.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3959..4972)
                     /locus_tag="IW715_002401"
     CDS             complement(3959..4972)
                     /locus_tag="IW715_002401"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1022983.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5137..6057
                     /locus_tag="IW715_002402"
     CDS             5137..6057
                     /locus_tag="IW715_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1022984.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1880 a         1214 c         1094 g         1936 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtattt
     1501 tccctctatt ttgttatcat taaagataac aaaatagtaa attggaaagg aacagttatg
     1561 aagaaaaata aagttatttt aagtattagt ttagcagcag cattgctgtt tgctgctggt
     1621 tgttctgcaa acaacacgac ttcttctagt tcgtctacag aaagttcaaa aagtacatct
     1681 actgcggaaa gcacgactgc cagtagcgtc gatttgaacg cattggaatt accacaattg
     1741 acgaaagatg tcgccgaaaa tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc
     1801 aaaatcaaat tatttcccga acacgcacca aaagcggttg aaaactttat gacacatgca
     1861 aaagacggtt actacaacgg actgactttc catcgcgtca tcaaagattt catgatccaa
     1921 ggtggcgatc ctaaaggcga tggcactgga ggggaaagca tttggggaga aggatttgaa
     1981 acagagatca acaaccacct ttataatata agaggtgcgt tatctatggc tcgctcacaa
     2041 gatcctaaca gtaacggcag ccagttcttt atcgttcaaa atacagatga tatgcatgat
     2101 ggtcttttaa aagacgacta tcctcaagca atcatcgacg cttataaaaa cggcggttat
     2161 ccttcactag atggccatta tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg
     2221 gatgctattg caaatatcga aacagatagt tcagacaaac caaaagaaga agtaaaaatc
     2281 gataaaatca atatactgca agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg
     2341 catagatgac aacgtatgct aggattcagg cgtcttcttt ttacataaaa agcaagtaag
     2401 cttccttttg ttttgctata tcaggtttta tagtcaagca ttttggttga cgatgaacgt
     2461 gaactcgcac tcacagactt ttttatcatc tacatatgct gcagataacg ctgtaccgac
     2521 tgatcccttc attttggtga tttcaaaatg acatttcagc acatctcctg gaacaacttt
     2581 ttgacggaat ttggccttat tgatcccacc gatataagct ctttttccta gaaactcttc
     2641 tgattttagg ataagtatcg aacctgcttg tgccagtgtt tctagaatca acgcacccgg
     2701 catcactgga ttgtcaggaa agtgaccttg gaaaaaatct tcattgattg ttacattttt
     2761 tgtcgcaacg atcttttttc ctggttctaa agtatctaca taatcaatat aacaaatagg
     2821 ataacgatta ggaatcaatt ccattacttc agtagcagat aatacgtaag acataattaa
     2881 actccttctt tcaggcaatt gccattttca ttactttgac tatcaaagta tcatattcct
     2941 aaatatttag caatcttttt ttaatttaag aaaaaatagt ttgacaatca aaacattttg
     3001 actaataatt aagaaatcaa gaagttattt tagctattat tctctatttt tttacgacta
     3061 tcaaattttt atcagaaaca aaggagagtc attatgtcat ttctagaagg aaaaaaaatc
     3121 gtcattatgg gcgtcgctaa caaaaaaagc attgcttggg gttgtgctga agcaatgttg
     3181 aaacaagggg cagaaattat ctatacttac caaaatgaac gaatgaaaaa atcattagtc
     3241 aaattggtcg gcgaagaagc attcactgta gaatgtgatg tttctagcga cgaaagcatc
     3301 gaaaaagcaa tgaatgccat cggtgaatat gctggggaga tccacggcct tgtccatgca
     3361 gttgcttacg ccaacaaaga agaattgtcc ggaaatgtgt ctgatatttc cagagacggc
     3421 tatcaattag ctcaagatat cagcagctac tctttaattg ctgtatcaaa atacgcacaa
     3481 ccgtttcttg caccaaacag cgggatcgtt agcatgactt atctaggttc agaacgcgca
     3541 atcccgaatt ataacatgat gggtattgcg aaagcttctc ttgaagctgc tatccgttat
     3601 ttggcagctg agttctcgcc taaaggaatt cgggtcaatg gaatctcagc tggggcaatc
     3661 aaaacattag ctgtcacagg tgttaaagac tatcaaaaac tgatccagtt atctgaatct
     3721 cgtacgcctg atcagcaagg tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta
     3781 atcagcccac tttccagcgg aatcatcggt gatattatct acgttgacaa aggtgtccat
     3841 ctttcataaa cttttgttgc attctttaaa gtaaaacata taaaaatcag gaaaaaactt
     3901 tttaaagtct cttcctgatt ttttcttttt acgttttttt caactcatta ctttgaaatt
     3961 attccacgac ttcaatccag ccttctggtg cttctatgtc tccaaattga atacctgtca
     4021 attcatcata caaccgtttt gttactggtc ctacttccgt ttcactataa aatacatgaa
     4081 gattgttatt gtgataaatc ccaccgattg gagaaataac agccgctgta ccacatgcac
     4141 cagcttctgc aaattgatct agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc
     4201 ctagtcgttc ttcagctaac tgcaacaagg aatatttcgt gatacttggt aagatagagg
     4261 gcgattgcgg agtgatgaat tttccgtctt ttgtaattcc aaagaaattt gctgcaccta
     4321 cttcttcgat tttcgtatgt gtggctggat caagataaac acaatctgag taatccagtt
     4381 cttttgcttc tgttcctgga agtaaactcg ctgcgtaatt tccgccgact tttgctgcac
     4441 cagtcccctt aggagcagca cgatcgtaat cagatacaat aaaattcgtt ggttttagtc
     4501 cacctttgaa atacgctcct actggcgtac agaagacgac aaataaatat tctggagctg
     4561 gatggacacc aatgttttca ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc
     4621 ctgttccata gggcggcaca aaatcttcat ttgcttttac cacttgcttg acagcatcaa
     4681 cgaacatctt ttcagggacc attggcatca gcaatcgtct agcactttga ttcatccgtc
     4741 gactattttg ttcaggtcga aagagattga ccttgccgtc ttttcgacga taagctttta
     4801 gaccttcaaa acattgctga ccataatgaa gcgctggaga gccttcatga atcgtcaggt
     4861 aattatcttc tgtcaattct ccttcttccc attgtccgtc tttccatttg gcaataaaac
     4921 gccaaggagt tttgatataa gaaaaaccta gattgttcca atcgatttgc atatacttac
     4981 caccttcgtt attttctcca cattttatca ttatccatat tatttgtcat tttattttca
     5041 gcttttttcc ttcattatag ttatttttgc tatttccttt tttgaaaact tcttggtaca
     5101 atacagaaag aatcaaattt tagaaaagag gattttatgt tttcattatt agcaactgcc
     5161 ggtacgacag actcttctgg cgagacattg acagatcaag ctgtcagcaa agtaaatgcg
     5221 tttcagcggt tttgggacgg gattgattgg gacgccattt tggctgcctt tatccaaaaa
     5281 agtttatatc tgattttttt gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat
     5341 ctgattgata agtcttttaa tcagtattct aaaaaagcca ttctaaacga gagccgctta
     5401 aagactttac atagtctttt aaatactatt tttcattata cccttggttt cttttttatc
     5461 tatgcaattt tatctgctat cggtgtgccg atcggatcac ttttggctgg tgccggaatt
     5521 gcaggtgtgg caatcggtct tggtgcccaa gggttcatga gtgatgttat taccggattt
     5581 tttattatca tggaacaaca gatggatgtt ggggactaca tcaagctagc aaacttaacg
     5641 attgaaggaa ccgtcgtatc tgttggtatc cgtacccttc agctgaaagc aacggatggt
     5701 actgtccatt tcatcccgaa tcgaaacatc accacgatca gcaatctctc tcgtgcaaat
     5761 atgcaagtac ttttagatat tcggattgtt cctgaagaag gatatgacaa aatctatgag
     5821 atcattgatc gagtaaatcg tacattagct gaaaaatacc aagaagagct ccaaactgaa
     5881 ccttctatct ttggattagt ggatcttgga aacagcaatt ttgcgattcg tacggtctgc
     5941 tacgcattga acggaaaaca atatgcctta aaagaagagt tcttgagctc ctatgtcaaa
     6001 gaattgacag cttctggttt cacgattccg aacagtccaa ttgctgtcgg aaaataattt
     6061 ggacaaacga tgcaacgaat ctcactaaaa agagcgtggg acaaaagtaa agaacacttt
     6121 tgtc
//
LOCUS       DACEMK010000119         5907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEMK010000119 DACEMK010000000
VERSION     DACEMK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.124"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..667)
                     /locus_tag="IW715_002403"
     CDS             complement(95..667)
                     /locus_tag="IW715_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1022985.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW715_002404"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW715_002404"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1022986.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IW715_002405"
     CDS             complement(1352..1867)
                     /locus_tag="IW715_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1022987.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IW715_002406"
     CDS             complement(1869..2153)
                     /locus_tag="IW715_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1022988.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IW715_002407"
     CDS             complement(2187..3515)
                     /locus_tag="IW715_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1022989.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IW715_002408"
     CDS             complement(3529..3984)
                     /locus_tag="IW715_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1022990.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..>5907)
                     /locus_tag="IW715_002409"
     CDS             complement(3987..>5907)
                     /locus_tag="IW715_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1022991.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2005 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatttt
//
LOCUS       DACEMK010000120         5874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEMK010000120 DACEMK010000000
VERSION     DACEMK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.125"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            641..1375
                     /locus_tag="IW715_002410"
     CDS             641..1375
                     /locus_tag="IW715_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1022992.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IW715_002411"
     CDS             1391..2560
                     /locus_tag="IW715_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1022993.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IW715_002412"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IW715_002412"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1022994.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IW715_002413"
     CDS             3650..4576
                     /locus_tag="IW715_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1022995.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IW715_002414"
     CDS             4595..5845
                     /locus_tag="IW715_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1022996.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DACEMK010000121         5662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEMK010000121 DACEMK010000000
VERSION     DACEMK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.126"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(72..542)
                     /locus_tag="IW715_002415"
     CDS             complement(72..542)
                     /locus_tag="IW715_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1022997.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IW715_002416"
     CDS             872..2512
                     /locus_tag="IW715_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1022998.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IW715_002417"
     CDS             2666..3988
                     /locus_tag="IW715_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1022999.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4487)
                     /locus_tag="IW715_002418"
     CDS             complement(4056..4487)
                     /locus_tag="IW715_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023000.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4552..5388)
                     /locus_tag="IW715_002419"
     CDS             complement(4552..5388)
                     /locus_tag="IW715_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1023001.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1696 a         1127 c         1037 g         1802 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4201 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4261 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4321 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4381 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4441 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4501 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4561 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4621 gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg acaattactt gaaaaataaa
     4681 ggtcactttt tttggagaca aaaccctgct aaatcttgct ccccacaaac ctccaataac
     4741 agcagcgggg ataacataaa acaacataga taaatcaaag cgcataaagc tacttgttaa
     4801 agcaatcgtt accaattttg ccagttgaga aaagaaaatc gtagatagag aatatagtgt
     4861 tgcttctttt attggcaatg caaacatcag catcaacaaa gaaacattga ctggtccacc
     4921 accaatacct aaaaaactag ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt
     4981 ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa
     5041 ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg
     5101 taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac
     5161 ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac
     5221 aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc
     5281 gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc
     5341 aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct
     5401 ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa
     5461 acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aacgaaaaga
     5521 ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
     5581 ataattttcg tttggttgaa cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     5641 aagaggtcgt gagcctgccg tt
//
LOCUS       DACEMK010000122         5501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEMK010000122 DACEMK010000000
VERSION     DACEMK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.127"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(163..621)
                     /locus_tag="IW715_002420"
     CDS             complement(163..621)
                     /locus_tag="IW715_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1023002.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(634..1959)
                     /locus_tag="IW715_002421"
     CDS             complement(634..1959)
                     /locus_tag="IW715_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1023003.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1976..3388)
                     /locus_tag="IW715_002422"
     CDS             complement(1976..3388)
                     /locus_tag="IW715_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1023004.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3405..4169)
                     /locus_tag="IW715_002423"
     CDS             complement(3405..4169)
                     /locus_tag="IW715_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1023005.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4273..5148)
                     /locus_tag="IW715_002424"
     CDS             complement(4273..5148)
                     /locus_tag="IW715_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1023006.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5403..5487)
                     /locus_tag="IW715_002425"
     tRNA            complement(5403..5487)
                     /locus_tag="IW715_002425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5451..5453),aa:Leu,seq:caa)
BASE COUNT         1695 a         1153 c          806 g         1847 t
ORIGIN      
        1 agaatcctga aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca
       61 aaaatcaaaa tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat
      121 tcacccataa atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact
      181 atctttaatc tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga
      241 aactacaatg atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc
      301 taacagtgtc ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc
      361 ttcgagttca actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc
      421 gatcgttatg gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg
      481 gatatatcca tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac
      541 ctcgtctttt actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt
      601 tcttgtttta aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt
      661 gtttactttg atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg
      721 attcaatgta aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag
      781 taacttctct actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt
      841 tcttagtgta attggctcca caccagttgc accaacatat gccggtattt caacaactgc
      901 atctgaccgt agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg
      961 atcatcattc aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt
     1021 aaagttcaat acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt
     1081 tttctctcgt ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac
     1141 gaccatatca ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt
     1201 tttcgtgatg actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa
     1261 atcggcgact tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg
     1321 ttgcaattct ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt
     1381 aacgatccaa ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat
     1441 agtcatatca caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga
     1501 aacgatcgac tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat
     1561 atgatcgaca atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag
     1621 tccacacgtt tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg
     1681 catctccaat cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag
     1741 ctttggatca tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa
     1801 tttaacaatc aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc
     1861 agataatggc aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt
     1921 actcccgcct ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt
     1981 cttctaataa tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt
     2041 gataattttt ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt
     2101 cctcgaccat ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat
     2161 ccaggatatt ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat
     2221 ttttacttgc taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct
     2281 tagcttcacg tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt
     2341 aatacaaggc aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc
     2401 gaaaattgat gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga
     2461 ataagtaaca gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag
     2521 gagaaagaaa catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc
     2581 ctgcaccgct tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat
     2641 agatagctag acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa
     2701 acagtccagc ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct
     2761 tgctaggaga tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca
     2821 gtccaaaggg gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca
     2881 tatcaacgag cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc
     2941 ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc
     3001 ccgcaaaata agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg
     3061 ccgctactaa accggaaata attccaccaa aaatccccat attcacttct gggtccataa
     3121 tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt
     3181 tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc
     3241 caaaaacggc atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata
     3301 aacgcatgat caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa
     3361 gcttctgcac atttgattta acattcattt ttattcctcc tctattaaat atcctcatat
     3421 gttactaact caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa
     3481 aattcgactt ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc
     3541 aaatacgctg cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa
     3601 cttgccaaat attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct
     3661 acgacatcga aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag
     3721 ttgctccgaa ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca
     3781 tccaaagtat gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa
     3841 attttttgaa tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga
     3901 tgctgataat aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg
     3961 aggagtggtt tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc
     4021 tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc
     4081 aaaattcctc gctgataagc atccataatc ccataattca caccatggct atagccaaaa
     4141 tcatccgaat taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag
     4201 gcgcaaccaa ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt
     4261 tacgccctta ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc
     4321 ttgaatggca agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata
     4381 cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc
     4441 ctgataaatc aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag
     4501 tgattcttta tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt
     4561 catttctact atttttaagc tctttaaact attaggctct cctagctcag cattaatgat
     4621 atgcatagtg atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat
     4681 tcgctcgacc acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg
     4741 caacataatt gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg
     4801 atctgtcaat gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag
     4861 ttcaatcact tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact
     4921 gaatcgtttg atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc
     4981 aaatggctta ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat
     5041 tccatcgtca tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa
     5101 ttcacctcct ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt
     5161 ctccaaacaa tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc
     5221 ctaattgaat agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg
     5281 ttatcaatat cttttttact cgttttatga taatacttgt atcgaattga tttggaggta
     5341 attacataaa aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat
     5401 tataccggcg gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag
     5461 cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata a
//
LOCUS       DACEMK010000123         5353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEMK010000123 DACEMK010000000
VERSION     DACEMK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.128"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            20..994
                     /locus_tag="IW715_002426"
     CDS             20..994
                     /locus_tag="IW715_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1023007.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            976..1437
                     /locus_tag="IW715_002427"
     CDS             976..1437
                     /locus_tag="IW715_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1023008.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1487..1951
                     /locus_tag="IW715_002428"
     CDS             1487..1951
                     /locus_tag="IW715_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1023009.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKNLDQEVKIFVQQVPSSSIENVDFSK"
     gene            1965..2714
                     /locus_tag="IW715_002429"
     CDS             1965..2714
                     /locus_tag="IW715_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1023010.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2714..3544
                     /locus_tag="IW715_002430"
     CDS             2714..3544
                     /locus_tag="IW715_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1023011.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3544..4191
                     /locus_tag="IW715_002431"
     CDS             3544..4191
                     /locus_tag="IW715_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1023012.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4289..5065)
                     /locus_tag="IW715_002432"
     CDS             complement(4289..5065)
                     /locus_tag="IW715_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1023013.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5147..5290
                     /locus_tag="IW715_002433"
                     /pseudo
     CDS             5147..5290
                     /locus_tag="IW715_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1825 a          731 c          962 g         1835 t
ORIGIN      
        1 tagagtgagg gataaagaaa tgagacaact acacaaagaa cagattgaaa cagcaatggc
       61 agcgttaaag gaaaagacaa ttgatcatgt atattttaca gcatgtgggg gttcacaagc
      121 cgttttaatg gctggtcaat atctatttga taaagaatta actgtacctt gtcatgttta
      181 tactgctaac gaatttaact atgctatacc aaaaggatta tctgaaaatt ctttagttat
      241 cacttgttcc cattctggca caacaccaga aactgtagaa gcaacaaaaa aagcaagaga
      301 atttggagca atgactattt gtttatctaa tgaagaagga tctcctctat gggaagcagc
      361 tgaatatcct gttcactatg attggggtaa agatgttgat gctagtgatt taaataaagg
      421 aattctttat ggtttattat ttaatatttt acttgtttta gatggctcta aaaaatggga
      481 aacttgcttg aatgaatttg ataagttaaa tgatcttgtt attgctgaaa agagcaaatt
      541 tgaaataact gctaaagaat ggggtaaaaa ccaaaaacga gataaaatta tttacacaat
      601 tggtagcggt attaattacg gagaaattta ctccacagca gcttgctggt tcatggaaat
      661 gcaatggatt aattctaatg ctattcattc tggggaattt ttccatggtc cttttgaaat
      721 cactgattat gatgtaccat tcatcttagt gaaaagtatt ggagatacaa gatttttaga
      781 tcaacgggta gaagacttca ctaaaaaatt cagtaataaa ttaatggttt tagaccaagc
      841 tgattttgac ttagaatcta tagatagtgt agcgaaagaa tatattgcag ctgtacttag
      901 tggcgttgtt attcgccaaa tggtagaagc gattgctttt gaaagaggcc attccttaga
      961 tgttagacgc tatatgtggc aaatgaaata ttaattaata ggaggttgca aaagatggca
     1021 aatcaattat ttcacattgt gattacaaca cattctaccc tttgtgaggg ttatttaaag
     1081 gcaacaaaat taattttaat gaatgacgcc acaggtatta gtgctgttcc atttaaagaa
     1141 aatactagtc cagatgattt tgaggagagt atgaaagaaa taattagtca gcatcatttg
     1201 caacctctaa ttattttaac ggatttaatt ggaggaacac ctaataatgt ttctattaaa
     1261 cagttgaaca acaatagaat tcaaattatt gctgggatta atttacctct tttactggag
     1321 ttattaatgg cccagtcagc tggtaaatct atagaagaaa tagattttaa ggaattactg
     1381 aataactctc aaaaaagtat ggttcatatt aattcgcttt taaaagatgt cagttaaggt
     1441 ggcaatttct attctataaa ttttaaaaat tgaaggagga aatagaatga ttaaattagt
     1501 gcgtgtagac cacagattac ttcatggaca agtaatattt tcttggacta aacagtatga
     1561 tattaaccat attattgtgg ctgataacaa agttccagga gatccaatgt ctgtgatggc
     1621 tttgacaatt gctaaacctg ctgattgcaa attagacatt gttaaaatta gtgatgttaa
     1681 agggattatg gaaacatccc caaatgataa ttacatgatt ttaattaaag gacctaagga
     1741 agcttttgaa ttagttcaaa atttaccaga aattaaagaa attaattttg gcggtgtgtc
     1801 taagaaaagc gagtccaaac aatatggaaa ggcagtcttt ttaactcctg aagaattaga
     1861 agccacaaaa aaaaatcttg atcaagaagt gaaaatattt gtgcaacaag tcccatcttc
     1921 aagtattgaa aatgtagatt ttagtaagta aggggggaaa ataaatgtta ttaaaagcca
     1981 ttttattagg tttagttggt gtggtagctg ttattgactc caggttaatt ggtcgacaaa
     2041 atattggtag accactaatt ttgagtacac ttgccggact tgtgctaggt gacttgcata
     2101 cagggattgt tcttggggca tctttagaat taatttctat gggattcgtc tcaattgggg
     2161 cggctggacc tcctaatatg caacttggta gtattattgc cactgctttt gcaattttaa
     2221 ctaatagtag tacagaaaca gccttaacaa tagctatccc agtggctgta gctggtgaat
     2281 ttatgagtat tatcatgcgc atgataattg ctcaatttgg acatgctgca gataaagcca
     2341 ttgaaaataa taaatttaga aaagctcaaa ttattcatat ttattggtca tttattttta
     2401 atgcttttgt ttactttatt ccaatctttt taacagtata ttttggtgct gacgttgtgg
     2461 cagatttagt agataaaatt ccagaaatga ttacagacgc cttgactgtc tctggaaata
     2521 tgctttccgc attagggttt gcaatgctat taagcacaat gttgagtaaa aaattatatc
     2581 cttactttat ttttggattc tttattgttg cctattcagg tctttcacta attggcgtga
     2641 caatttttgc agctctgatt gcctttgtaa tggatcaagt taaatatgga aaaagagaag
     2701 aggcgcatgg ttaatggaaa atagtacaga aaaaaaacgt ttaatcacaa aaaaagatct
     2761 tcataagatg tgtcttcgtt catggacaat catgtgttcg tggaattatg aaagacaaat
     2821 gcatatggga tttatgtatg ggatggcgcc aattttaaat aaagtttata aaaatgatga
     2881 agagaaaaaa agagaagctt atactcgtca tatggcattt tttaactgta cgcctcaaat
     2941 gactccattt attatgggac tttgtgcttc tatggaagaa caaaatgctc aagcagaaga
     3001 aggaaacttt aatactgaat ctatctccat gattaaaacc agtttaatgg gtccttttgc
     3061 tggaattggt gattcatttt tccaaggtac tttaagaatt attacatttg ggattggatt
     3121 gtcttttgct aagcaaggta gtattttagg accgatttta gctgttggcc tttttgctat
     3181 accttctatt ttagctgttt tttacggcgg taaattagga tatgagtctg gcaataaata
     3241 ccttactaaa ttgtatcaag aaggtcttat ggatcgagta atgcaatttg cttctatcat
     3301 tggtttggct gtagtaggtg gaatggtggc tagcatggtg ccaattgtca cccctttaaa
     3361 attcactaca gggggaacag aacttgtttt acaagactta cttgattcca ttattcctgg
     3421 attattacca cttgtatgta ctttgggaat atataaattg attaagaaaa atgttagtac
     3481 aaatatgctc ttattaggtt gtattttggc agggtttgtt ttaagtataa taggtatctt
     3541 ataatgatta aggctctaat ttttgatatg gacggagttt taattgatag cgagattatg
     3601 tatcaaaagt gggtatttaa ttttttaaaa caagaaaatt taagctatcc tttgtattct
     3661 tatcaaaaaa agattggaac tacagcaagt atttttgatg atatagaaaa gtacaatgag
     3721 ggatgtaaca gtttagaatt gaagggaaaa tttactaaat attggaaatc gcaaaagatt
     3781 aattacttgg atatttttcc taatatacta attgaacagc ttgaatggtt aaaaaagaaa
     3841 aaaataaaaa tggcaattgc ttcttccagt ccaatggata ctataaatga agttgttacc
     3901 acttgtaaaa tagagtcata ttttgattgt ttaattagcg gaagagattt gccagaaagt
     3961 aaacctaatc ctactatatt ccttaaagct tcagaacagc tcagaattcc agttgaagaa
     4021 tgtctggtga tagaagattc ttataatggt ataaaagctg gtaaaagagc aaatatgaag
     4081 gttcttgcaa ttaaagataa acgtttttct caagatgtaa gtctagcaga tgatgttatt
     4141 tacgatatta aatatctgcg acaagaagta caggtgaaat ttggaatata aataagtttt
     4201 ttgatgtatt tagagaatat tagtaaaaat aaaaatcatg cccaaatcta atcaaaattg
     4261 attgctttgg gtatgatttc ttttaatatc aatctattac tttaggatct gaatcatttt
     4321 tatgcaccgt atctaaagtg atattttctg tgacttcaat aacataacta attctatcac
     4381 aacgaactgt ggattgagaa taatgaaatg gttgattatt tttatctttg ccaacctttt
     4441 ctaaaataaa tagcggatct cctactatac attgcaataa gtcggaaagc tcaggtgttg
     4501 cagataaacc gtttatttcc ataatagagc tcgtagcttt gacatcatag tcatcttcta
     4561 gaagtttata taatgaagta tattggctac ctttttcaaa taaagttgga aatttttctt
     4621 ctgatgcgaa aaaattatca attcccatag gggcaccgtc ttcccacatt aaacgtttaa
     4681 tattgtaaac tctatcagtt acattgattt gtaatttatt agctaaaaac tcatcagcct
     4741 ttataatttt agaatgtaaa acagtatatc taattttatg tcctcgggta gaaagagaat
     4801 cgctaaatcc atgaactcca gctaatttta aactttcttt tttttgagtt acaaaggtcc
     4861 ctttccccct taatcgttca actgttccac tttctgttaa aagtttaaaa gcttttcgaa
     4921 tggtaatctt acttactcca taaattttac ataattcttc ttctgtaggt aattgctctc
     4981 ctggaagata ttttttttca cgaatatcgt tacgtataga agcttctact tgtagatata
     5041 atggtctatt atcttgaaaa ttcatagtac actccctaaa aagttatact tcattatatc
     5101 acaatttatt aaaataaaag attaagatat aaatataaat gaactagtct ttgactaacc
     5161 aatgcccgct tttgaaagaa aacaaattca ttctgtttta agtaaaaatc cttatgttac
     5221 cactcattct gaaggggatg agccttatcg ctacttggtt gtggaaccaa gtgataagac
     5281 aattttataa attcaatatt aattaaaata aaaacaaaca aagtagatta ttttactttg
     5341 tttgtttttt tat
//
LOCUS       DACEMK010000124         5239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEMK010000124 DACEMK010000000
VERSION     DACEMK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.129"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1804
                     /locus_tag="IW715_002434"
     CDS             <1..1804
                     /locus_tag="IW715_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1023014.1"
                     /translation="LWINSLEKEAIYQGFQQLRDAEETYPKFEEAQARQIADWLIGMN
                     GSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNG
                     AFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSLQ
                     SKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKA
                     EAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVA
                     MFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTK
                     GEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATR
                     ANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQ
                     KGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCR
                     EQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGS
                     FEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            2045..2638
                     /locus_tag="IW715_002435"
     CDS             2045..2638
                     /locus_tag="IW715_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1023015.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            2651..3550
                     /locus_tag="IW715_002436"
     CDS             2651..3550
                     /locus_tag="IW715_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1023016.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            3553..4026
                     /gene="ssb"
                     /locus_tag="IW715_002437"
     CDS             3553..4026
                     /gene="ssb"
                     /locus_tag="IW715_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1023017.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            4392..4652
                     /locus_tag="IW715_002438"
     CDS             4392..4652
                     /locus_tag="IW715_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023018.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            5096..>5239
                     /locus_tag="IW715_002439"
     CDS             5096..>5239
                     /locus_tag="IW715_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1023019.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCG"
BASE COUNT         1937 a          932 c         1065 g         1305 t
ORIGIN      
        1 actttggatc aactccttag aaaaagaagc catttatcag ggattccagc agttacgaga
       61 cgcagaagag acctacccaa aatttgaaga agcacaagca cgccagatcg ccgattggtt
      121 gatcggcatg aacggtagcc cgctgtatag tttactcttg cagcaaaaag gcataccagg
      181 aagtttttcc ttaggcagag tccaaacccc aacgctttac atgatttatc aattacagga
      241 aaagattaga aacttccaaa aagaaccata ttttgaagga aaagcccaag taatcgctca
      301 aaatggcgca tttgatgcga agcttgatcc gaatgaaacc caagcgacgc aagaagcatt
      361 tgaagcctat ttaaaagaaa aaggtgttca attaggaaaa cagccaggta cgatccacca
      421 agttgaaacc gagaaaaaaa gtgcggcaag tccacgtctt ttttctctat ccagccttca
      481 atctaaaatg aatcagctca tgaaagctag tgccaaagat actttggaag ccatgcaagg
      541 gttgtatgaa gggaaatatc taagctaccc acgaacagat accccttata ttacagaagg
      601 ggagtatgcg tatctgttgg atcacttaga cgaatacaaa cactttttaa aggccgaagc
      661 cattcctact cctattcata caccgaatag tcgatatgtg aataataaga aagtccagga
      721 acattacgcg atcattccta caaagacggt catgacggca gcggcttttg aacaattaag
      781 tccactgcag caggctattt atgaacaagt gttaaaaaca acggtggcca tgtttgcgga
      841 aaagtatacc tatgaagaaa caacgatctt gacgcaagtt cagcagcttc agctaaagac
      901 agtcggaaaa gtaccagtag atctaggttg gaaaaagctg tttggcaaag agagcgacag
      961 aaaagaaaag gaagaagaac cgctacttcc taaagtgaca aaaggagaag cggtgacggt
     1021 agatctacaa gtccttgaaa aagaaacgaa gccaccacaa ccttataccg aaggtacgtt
     1081 gatcacagcg atgaaaacag ctggaaaaac cgtggatagc gaagaagcac aatccatatt
     1141 gaaagaagta gaaggaatcg gtacagaagc aacacgagca aatattattg aaactttgaa
     1201 acaaaaggaa tatatcaagg tagaaaaaaa taagcttgtg gtgacaaata aagggatttt
     1261 actttgccaa gccgtggaaa aagagccgct gctaacaagt gccgaaatga ccgccaaatg
     1321 ggaaagttac ttattaaaga ttggtgagca gaaaggaaca caagctactt tcttagctaa
     1381 tatccaaaaa tttgtctctc acttgttgga agtcgtaccg ggacaaatcc agtcaacaga
     1441 ttttggatca acgttacaag aggtgaaagc agccagtgaa aaacaggaag caacgcgtca
     1501 tttaggtgtt tgtccaaaat gtcgggaaca agaagtcttg ctttatcaaa atgtggcggc
     1561 atgtacgtct gaagcctgtg attttaaatt atggacaacg attgccaaaa agaaactcac
     1621 agccacacaa ctgaaagaga tcattcaaaa cggccgaaca agccaatcag tcagaggact
     1681 aaaaggtcag aaaggttcgt ttgaagcaac gatcgtttta aaagaagact ttacaacagg
     1741 ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa agaacacgcc gcactacaaa
     1801 ataatgaagg ggttatccta tgcgaaatac aacacacaaa caacaagaaa tcgaaaatca
     1861 aaaagcaaaa attcgtttag aacaagagaa attacgtgca aataaaagaa gactgcaaca
     1921 attgaagaac cagcagcgca ttcaaaaaga aatcgaatta gatcgataga cgttaatctt
     1981 gttacaaata aactactatg atatatttga tgtagtgtat ttgtaacgaa gggaggggaa
     2041 gtaaatgcaa gataaactga aagaattgtt ggctattcgc gatggaaatt tatctatgaa
     2101 ggaagccagg aaatttggaa tcgcagcaac cactgtacaa agattggtga atcaaggaaa
     2161 attgatcaaa gttgatcgtg gttattatgt agaggatggt catggaatag atgatttatt
     2221 ttggcttcag caacgatttt ccaggggaat cttctctcat gaaacgactt tagatattta
     2281 ccagctttct acgaatatgc cgaaattcat tcacttaact tttccccatg gctataacgt
     2341 tgatcgttca attacaaaag agcagcagtt acaatttcat ttcgtgaaga aagaagttta
     2401 tgaactagga aaagtagagg gaacttcctt tcaaggaaat cctattacta tgtatgataa
     2461 agagcgtaca ctttgtgata tttggaatcc gagatatgaa tttgaatatg aaatgaaatt
     2521 agaagcatta aaagagtaca tggaagaccc attgagagat ccaagtaaat taagagatta
     2581 catgacaaaa ctacatgtag aaaaagaaat gaaataccac atgcaaagct tatattgatg
     2641 gaggaaaaag atgacaccga cacaatttgc agcaaaaaca agaaatttag caaagagtac
     2701 aggattaaat gcacagttag tgcaacggca ttttatgatg gatcgtctaa ttgaacaaat
     2761 ttctgaatca aagtacaaaa atgacttcat tttgaaaggc ggctttttat taggatcaaa
     2821 atatggaatc gatagaagga caacaattga tatcgatacg acatttagaa atagcaaatt
     2881 aactgaagaa aaattgaaga gcatttttaa tgatttaact agaaaaccca caaaagaagg
     2941 cattgttttc tctattcaag gaatgaagga aacaagagaa gccgattttt atcctggatt
     3001 tcaaattaaa ttggtggcta atttagagaa agtacgtgta ccgtttaaaa tggacgtgac
     3061 aacaggagat tctatttatc ctgaagtaga tacacactat caccaattaa tgtttgaaga
     3121 taaaaaagtg gagattccag cttatccaac agaacaaatt attgcagaaa agctaagtgc
     3181 tacgttcagc tttggcacag ataattcgag agcaaaagat tactacgact tatttaccat
     3241 tccgaaacta gaaaatatcg atacgaaaga gttatacaaa agtgttcgaa atacatttac
     3301 aaaacgtggg gatacaaaac cgttgtcctt ttattatgaa agagaaatgg ataagatcag
     3361 aggaaatgac tatttggaaa agcagtggga aaaatatcgc tcagttaaca cgtttgcgaa
     3421 ttccatttca tttacagata cagtcaacga aattgatcac ctaatgaaaa gtgtgatcaa
     3481 aattgaaaat caagaaagaa aacggcagtt taaaagatca caacaaatga atcaagaaat
     3541 ggagagataa agatgattaa taatgtgaca ttagtaggac gcttaaccaa agatcccgat
     3601 ttacgttata ctgcaagtgg cacagcagta gcgactttta ccctagccgt caaccgaaat
     3661 ttcgcgaatc aaaacggcaa tcgagaagcc gattttatta actgcgtgat ttggcgcaaa
     3721 ccagcagaaa caatggcgac attagctaaa aaaggcggct tgatcggtgt tgtcggacgg
     3781 attcaaacac gaacttatga caaccagcaa ggacaacgtg tgtatgtgac tgaagttgta
     3841 gcagataact ttcaactatt agaaagtaaa gcagcgacag agagccgtgc gcacgctgat
     3901 caatcaacca ccagtccaag ccccactacc tttgaacaac gggatacaaa caatggattg
     3961 gacgcttcac aaaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctacca
     4021 ttctaagtag cattttctat tggttgcatt tgctacggtt tatgttataa tttagtcaac
     4081 aaaaagaatt ttcaatctat aaccaaaaac cactagctgg cgggctagtg gttttctttt
     4141 acgtttctat tataagaatt aacgggtagg cttactgtct ttcttggcaa atttacaatc
     4201 aagccatttg cagatatagt gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa
     4261 aagaattttc aaacctataa cctcctcccc ttgccagatt aggccggggt agcagacagc
     4321 aagactatta tagcataatt gaaatattca aatgattgga tttttgctat aattttagcg
     4381 aggtgattgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacgc
     4441 aaccgttatt ggttcgcttt actttacaaa cgattcatga atcgattaat tgtgtagttg
     4501 cagatattga aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg
     4561 tcacaggtca cttcaataaa cgaaaccagc tagttattgc tagcatgtat gttagaaatc
     4621 ccgatcagtt tacgcgattg atgggcatat aaaaaaagaa gagttgcaat cctaatgtag
     4681 gactgcagct ctttttgttt tacatagaaa caagaaaaat gaaaaggcct ttttaggaag
     4741 gcgcgagcgc cttctcaatt gaaaaacctc tctcactcca accgccaccg ccgttcgaga
     4801 agaaaggaag tgaacgttgc acgttcacta agataaaggg tcttgcaaag caagacaaaa
     4861 tagaaaaaac ttcctctcac caaaaggttc gttcctgttc gttatctctt tattgatttg
     4921 ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc
     4981 acgctgcgcg cccttgtctt gtcgcttttc gttgattcat tctggccaac aaatcaataa
     5041 agaaagaact aacgaaagga agtaaaacct ttcctaagag gaggcagaat caatcatgcg
     5101 aaagaaattt gattattggg gtgttccctt cagcgaatta ttcccaaact atcatgcacc
     5161 acatactgtc gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct atcgtttgta
     5221 tcaatgtcca acatgcggg
//
LOCUS       DACEMK010000125         5238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEMK010000125 DACEMK010000000
VERSION     DACEMK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.130"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW715_002440"
     CDS             <1..1023
                     /locus_tag="IW715_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1023020.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="IW715_002441"
     CDS             1157..2500
                     /locus_tag="IW715_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1023021.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2612..4015
                     /locus_tag="IW715_002442"
     CDS             2612..4015
                     /locus_tag="IW715_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1023022.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            4364..4996
                     /locus_tag="IW715_002443"
     CDS             4364..4996
                     /locus_tag="IW715_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1023023.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         2025 a          702 c          998 g         1513 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctgggaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2641 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2701 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     2761 aacagcagag agtagtgaac ttttgacaac ttcatcaagt gaaagtagta cgacttcaaa
     2821 tgccgagtct tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc
     2881 aacaacttcg tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag
     2941 tagtacaagt gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa
     3001 aaaggaagcg ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga
     3061 atcaacagaa tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga
     3121 caaaaatgaa tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg
     3181 tcagaaatat gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag
     3241 tggtcaaagc cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta
     3301 taatggaaac tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac
     3361 gacccaatca aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa
     3421 attattaact aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa
     3481 tagtaaaaca tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca
     3541 atactatttg aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga
     3601 agttaaaggt ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat
     3661 aaccagtttg tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac
     3721 acagggtgag ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta
     3781 ctcttgggga aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca
     3841 ccagcaacaa tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta
     3901 tgtaggaagt actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag
     3961 tagtctttct cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag
     4021 gtaatcaatc tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata
     4081 ttgagactgg aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat
     4141 cgaatatcga gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat
     4201 gaaaacaatt tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa
     4261 ttagtcataa aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt
     4321 aattgaaatt atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg
     4381 tcaccttact gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg
     4441 tgactgtggg aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag
     4501 ttgtggatgt ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt
     4561 tggtaagtta gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga
     4621 atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga
     4681 tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg
     4741 attacttact gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg
     4801 tcattgtgag tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac
     4861 tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat
     4921 agagtgtcta acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg
     4981 ttctaaagag aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc
     5041 gtatgattct attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca
     5101 acctttgatt gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga
     5161 tatataaaca tctataatat attattcata ctattgatat ttagtataat cctatggaat
     5221 tgaatatcat agcaatgt
//
LOCUS       DACEMK010000126         5168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEMK010000126 DACEMK010000000
VERSION     DACEMK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.131"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..348)
                     /locus_tag="IW715_002444"
     CDS             complement(<1..348)
                     /locus_tag="IW715_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1023024.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTE"
     gene            683..1117
                     /locus_tag="IW715_002445"
     CDS             683..1117
                     /locus_tag="IW715_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1023025.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1140..1460
                     /locus_tag="IW715_002446"
     CDS             1140..1460
                     /locus_tag="IW715_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023026.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1601..1792
                     /locus_tag="IW715_002447"
     CDS             1601..1792
                     /locus_tag="IW715_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023027.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1863..2120)
                     /locus_tag="IW715_002448"
     CDS             complement(1863..2120)
                     /locus_tag="IW715_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1023028.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2278..2541
                     /locus_tag="IW715_002449"
     CDS             2278..2541
                     /locus_tag="IW715_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023029.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2565..2753
                     /locus_tag="IW715_002450"
     CDS             2565..2753
                     /locus_tag="IW715_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023030.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2836..3021)
                     /locus_tag="IW715_002451"
     CDS             complement(2836..3021)
                     /locus_tag="IW715_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023031.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3236..3607
                     /locus_tag="IW715_002452"
     CDS             3236..3607
                     /locus_tag="IW715_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1023032.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3757..3829)
                     /locus_tag="IW715_002453"
     tRNA            complement(3757..3829)
                     /locus_tag="IW715_002453"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3794..3796),aa:Gln,seq:ctg)
     gene            complement(3875..4303)
                     /locus_tag="IW715_002454"
     CDS             complement(3875..4303)
                     /locus_tag="IW715_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023033.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4363..5121)
                     /locus_tag="IW715_002455"
     CDS             complement(4363..5121)
                     /locus_tag="IW715_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1023034.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1776 a          814 c         1022 g         1556 t
ORIGIN      
        1 ttccgtttga accgtttctt gtacaacagg ttgttcagct tgaacaggtt cagcttggac
       61 tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc cttctgttgg
      121 gattgttaat tgttgtcctg agtagatcag gttaatatca gaaattgaat ttgattctgc
      181 aatggcattg atcaaggagt tatcaccaac gtatttttga gaaatcgttg atagagtatc
      241 acctgattga acggtataag cttcgtccgc atgagcagtc gttcccatga agaatgcagc
      301 gccggcagcc aaagttgttc caaaaagtaa agttttaagt gatgtcatga aaaatctcct
      361 ttattcgtta aaaagtttta tttgttgtgt tcaacgagga atactttaac acgtgaaaat
      421 atttcatagg taaaagaagc atgacaattt atgacaaatc cacgtttttt gtaacagaat
      481 gatgttcgct cgatgtgaaa aatgatgagg aatttcacat aatgcagaaa taatgggatt
      541 agaacaggtg ttctgatgag aaaagaaatg tataaataac ttgaaagaaa ttgcttttgt
      601 tcatcatttt atttacatat tgcgtatatt ttttgtattt ttgcttgaga tgttacaata
      661 aaagaaaaag gaggcggttc caatgagtaa aagataccag aacatcatgg tagcagttga
      721 cggatcaaga caatcgatca aagcgttcgc tgaagccctt gacttagcaa aggacaatga
      781 agcccactta tttatcgtgt caatcatcaa caaagttgag ctgacccata gtgcctatgc
      841 cttttcaaaa atctatgcag atgaaaaaca aagaacagaa gtagccatgc tgaaaaaaat
      901 caatgatgcc aaagagtacg gcattgctga gatccacgca atagttgaaa ctggagatcc
      961 aagaaatttg attgccaatg tcatccctgg acaagaaaat attgatttga tcgttatggg
     1021 agcgacagga aaaggtgcta tacaacaagc attagttgga tcgaccgctt cttatgtcgt
     1081 atcacatgcc ccttgcagtg tgttggtcgt gaagtaatga gataagggag gaatagaaaa
     1141 tggttcgtcc tcaagaagtc cgtgcgccaa aagaaaaaat agaaatttta gcaattattg
     1201 aagacggcac acaaacaaag aagggatact cgatcgcttt agtaaagtgg aaaggaaaaa
     1261 aaggtgttgc tctgagatgg gatggagata accaacaaga taaaggattc cctgtcacag
     1321 ctaacggtta tcatccagcg tggtttgttc tgcctgataa atttatggaa ttatacagct
     1381 atgattatgc aagagccgca gcatcaatca aagcattaga taaattaaca gaagagcaga
     1441 atgaaatgga ggaaaaataa aaaagacgaa ccaatcagcc tttagaaaac tgattagttc
     1501 gaggagtaaa aatgaaaaaa ggttatttgg gatgaaacta gaataatgcg tataacttaa
     1561 ataaaactta aagcaataaa atggcaagag ggtgaggcaa atggaagcag gaaaagtatt
     1621 acaggaagta aaagcactat gtgcagataa aaaatatgaa gaagcgaaaa gcctgatcga
     1681 aaagaacaaa gaaaatttgg aagaaaagta cccgatcgca caagaattta tagaattaaa
     1741 acaaggaacg atccttcacc gattcaaaca tttttttggt ataacagaat agaaaaaacg
     1801 attaaagatc ggcagaaatt tgctgcatct ttaatcgttt tctttttaaa catgcgaggg
     1861 attcaattct gattcttata taaaaaatag atccaaataa taagaaaaat tactgaaagc
     1921 gtcaattcga tgatgtgtgt tattaataca gacgttgttt cccaatgcct aaaagaaaaa
     1981 atttgataag taaaatatac agcaggtaaa gaggccagta aaactgaaaa cttctttttt
     2041 gtccatttta tgtaatagaa aagaatgctt atcataataa aaaaaggaat ataaaaaaca
     2101 aattgaccga tcgcgatcat actattgcct ccgataaata aagtccatga tacaaaataa
     2161 cactttgtgc gaaatggcgc aagcagtttt gtggatttat ccggtaaaat ctgtatcgat
     2221 tttgaaatta gaggagaaac ttgctataat caaagtgaaa gaaggagtgg gattttcatg
     2281 gatcatgtag tgttagaaat cgatttgcat ttgatcaata atattcgggt gatttactat
     2341 gttgtaagta acagtgtcga acaacgtgta ctgacgaata aaataaatgg tattttagca
     2401 aagaaagacg ttcaccgatt caataatggt gaaggaagtt attatagtat cccagtagaa
     2461 aaaattattt atacgacagt caaagtcaga gaagatctag ataccaaaaa agcatatgaa
     2521 cctattttta caacttattg attgacagca gatggaggaa aaaaatgaat ccatatgaac
     2581 aacttattca agatttgatc gatggaaaaa tcgaaaagat tgatgtaaaa agagaagaat
     2641 taatggcttt tcgagaagcg tggctgaagt tggagaatcg taaatatgtg gtgggaaaag
     2701 cctcactgaa cggcaatgtg acttatcatt acgatccgac acgtttattt tagtatgaag
     2761 tattcagaaa agaaccaaag gaggacgatg cagcagcatt gtcctccttt gattttacaa
     2821 aagtgaaagt tttacttatt tgaatagatt tttaaatttg tttttgacac tgtcgatatt
     2881 tacatctttt aaaagatttt gggctttctc tttataaggt cctaattgtt ctttatgttc
     2941 ttcgataaat cgttttgcgc catcgaaatt gccgttctta atcatttcct tgactttttg
     3001 aaataattct tcttgattca ttttgaacac atcctttgct atcaacatga ttttttacat
     3061 ggacaaaacg agaaaaactg atttatccac acgtcaataa tatcaaataa cgatcatttt
     3121 tactaaaaaa acactcgatt ctcgcttcta aagtaggaga aaaatctcca acttgggacg
     3181 gatggaattt ctttaactga cccatatact gaatatataa caaggaggta gaaagatgaa
     3241 agtgattaaa tatttgattg gactggcggc gattagtggt attttgctat ttggactgaa
     3301 atattatact gaaggaagtt atggagaaat ccctggtata cttgatcagc taaatccatt
     3361 agttgaaaaa ggagaggtct atgtaaagac acaaaaacca gaggaagtaa atgaatatgg
     3421 tacagcaagg tatgtgcaga aagctgctga tgccaatggg aaagaacgaa cggttgaatt
     3481 taatggtctt tctgtgttaa aagaaaatca ttatttgaaa attagcaata aaggagcaca
     3541 catagaaacg tatgaggaag ttcctagaga aaaagttccg gagaaagcat tagcaattat
     3601 tgggtaaatc agtttttgta ttaagaatac gcccgatttt attattcata atttaattat
     3661 gtaaactaaa tacaagtagt aaaaataata caataatccc cctgcaaaaa gattgttctt
     3721 ttacaggggg ttcatttttt cagcaagcac atcaaattgg gatggcaggg ttcgaaccta
     3781 caattgcttg attcagagtc aagtgcctta ccagtttggc tacatcccat ttattcaaca
     3841 aaataatcat agcactttcc taaaaaaacg caagttattt ttcttgatcg gatagttgtt
     3901 gctttactcg taaaaggcgg cgtactagtt tcttttctaa tccagcagga agggcaatca
     3961 gttctgctac tgacaagacg gtgtccaata actgatattc tttgacaggt tcattcataa
     4021 tatgatagat gtttgctaaa cgttcaatat attcaaagcg agtatactct gttcgagcaa
     4081 ttagaaattt caataaatcg gtggctttct cgtactcttg atctgcgtaa taaacaagag
     4141 attgacgata caatttgatt tccagttcat tgagggtagc aggtctgcga cgctcgttcc
     4201 actcattaat agagacttta ccttgaacat ttcccttgca gtcacggaca aaaataatcg
     4261 gaatttcatt tggatccatt tttttatgtg tgaatttttc cattatctac gctcccttca
     4321 aaggtttggt atcttgagta taagggattt ttgtttgaat tactagcttt tctccaaagt
     4381 tagcatctgt ccacccattg cagtgaaaat cgccgaaaaa tcgttgatcg gtactgctcg
     4441 attttttaca ccatagggat cgttgacata aatattttct ttgtcatatc cagtgactac
     4501 acaagcatga acaagaggag tgactcttac ttctccatta tttgtggtcc aagtcataaa
     4561 atcagtttca tctgggactt caaaattgat agtcacagaa gtccagacag gatttccttt
     4621 agatagtgct tttttgatat catcgagttg agcatcggtt tttggaaaag tgtgataatc
     4681 cgtttggacg atttgctgag caagggaggc aactggggga acatagacga ccatagccca
     4741 gtctccgcca ctgatatcac caacaaatcc ttcattagga tcgccatgag tcccatctgc
     4801 attgaaaaca gggacgtaat ccaattgctc agctaactgg tttttagttg tttcataacc
     4861 ataaaaagat aaaagcatag aaagcgaagt gacttcacag ccattttcta aagaaggaga
     4921 atcaaactgg ctctctaatg gaacatctag taaaacttct gctttctctt ttgtcttttt
     4981 cgttttttgt gttgaacttt tttgtgcagt gttcattgta tgttctggag aatggatatg
     5041 taggtaataa cttgtaccgc ttaataaaag agcagctgtg ccccctagaa acaatagttt
     5101 tttgaacgtt tttttcttca tttgaaatct cccttttcag ctgtttatct ttcctattat
     5161 aaatgtat
//
LOCUS       DACEMK010000127         5021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEMK010000127 DACEMK010000000
VERSION     DACEMK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.132"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(207..1169)
                     /locus_tag="IW715_002456"
     CDS             complement(207..1169)
                     /locus_tag="IW715_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1023035.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IW715_002457"
     CDS             complement(1171..2610)
                     /locus_tag="IW715_002457"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1023036.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IW715_002458"
     CDS             2795..4741
                     /locus_tag="IW715_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1023037.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1630 a          837 c          813 g         1741 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa t
//
LOCUS       DACEMK010000128         4898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEMK010000128 DACEMK010000000
VERSION     DACEMK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.133"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IW715_002459"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="IW715_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1023038.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="IW715_002460"
     CDS             complement(2066..3064)
                     /locus_tag="IW715_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1023039.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="IW715_002461"
     CDS             complement(3290..3943)
                     /locus_tag="IW715_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1023040.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IW715_002462"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="IW715_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1023041.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1447 a         1020 c          753 g         1678 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttc
//
LOCUS       DACEMK010000129         4807 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEMK010000129 DACEMK010000000
VERSION     DACEMK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.134"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IW715_002463"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IW715_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1023042.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IW715_002464"
     CDS             complement(3250..3768)
                     /locus_tag="IW715_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1023043.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1579 a          786 c          730 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACEMK010000130         4726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEMK010000130 DACEMK010000000
VERSION     DACEMK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.135"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IW715_002465"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IW715_002465"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1023044.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IW715_002466"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IW715_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1023045.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IW715_002467"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IW715_002467"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1023046.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DACEMK010000131         4571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEMK010000131 DACEMK010000000
VERSION     DACEMK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.136"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(338..1291)
                     /locus_tag="IW715_002468"
     CDS             complement(338..1291)
                     /locus_tag="IW715_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1023047.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="IW715_002469"
     CDS             complement(1314..2069)
                     /locus_tag="IW715_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1023048.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="IW715_002470"
     CDS             complement(2066..3025)
                     /locus_tag="IW715_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1023049.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="IW715_002471"
     CDS             complement(3018..3968)
                     /locus_tag="IW715_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1023050.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="IW715_002472"
     CDS             4118..4570
                     /locus_tag="IW715_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1023051.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1490 a          962 c          775 g         1344 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt cacgttgaaa
      181 aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc tgattgattt
      961 tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt ggcacctcga
     1141 tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta ctgcttttct
     1201 ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc gtacaattcg
     1381 tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa ctgcccatct
     1441 ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa a
//
LOCUS       DACEMK010000132         4466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEMK010000132 DACEMK010000000
VERSION     DACEMK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.138"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            162..392
                     /locus_tag="IW715_002473"
     CDS             162..392
                     /locus_tag="IW715_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1023052.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IW715_002474"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IW715_002474"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1023053.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IW715_002475"
     CDS             1803..1979
                     /locus_tag="IW715_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1023054.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IW715_002476"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IW715_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1023055.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="IW715_002477"
     CDS             2594..3061
                     /locus_tag="IW715_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023056.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="IW715_002478"
     CDS             3146..4030
                     /locus_tag="IW715_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1023057.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4147..4380
                     /locus_tag="IW715_002479"
     CDS             4147..4380
                     /locus_tag="IW715_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1023058.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1524 a          678 c          970 g         1294 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa accttt
//
LOCUS       DACEMK010000133         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEMK010000133 DACEMK010000000
VERSION     DACEMK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.139"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            9..911
                     /gene="vanY"
                     /locus_tag="IW715_002480"
     CDS             9..911
                     /gene="vanY"
                     /locus_tag="IW715_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017153051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ1023059.1"
                     /translation="MFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLENS
                     GTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSN
                     IYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEH
                     NSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVG
                     LPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYE
                     ISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1064..1549
                     /gene="vanZ"
                     /locus_tag="IW715_002481"
     CDS             1064..1549
                     /gene="vanZ"
                     /locus_tag="IW715_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1023060.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1836..2165)
                     /locus_tag="IW715_002482"
     CDS             complement(1836..2165)
                     /locus_tag="IW715_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1023061.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2470..2799
                     /locus_tag="IW715_002483"
     CDS             2470..2799
                     /locus_tag="IW715_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1023062.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2789..3076
                     /locus_tag="IW715_002484"
     CDS             2789..3076
                     /locus_tag="IW715_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1023063.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3355..3918)
                     /locus_tag="IW715_002485"
     CDS             complement(3355..3918)
                     /locus_tag="IW715_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1023064.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1569 a          665 c          747 g         1480 t
ORIGIN      
        1 tgaagaagtt gtttttttta ttgttattgt tattcttaat atacttaggt tatgactacg
       61 ttaatgaagc actgttttct caggaaaaag tcgaatttca aaattatgat caaaatccca
      121 aagaacattt agaaaatagt gggacttctg aaaataccca agagaaaaca attacagaag
      181 aacaggttta tcaaggaaat ctgctattaa tcaatagtaa atatcctgtt cgccaagaaa
      241 gtgtgaagtc agatatcgtg aatttatcta aacatgacga attaataaat ggatacgggt
      301 tgcttgatag taatatttat atgtcaaaag aaatagcaca aaaattttca gagatggtca
      361 atgatgctgt aaagggtggc gttagtcatt ttattattaa tagtggctat cgagactttg
      421 atgagcaaag tgtgctttac caagaaatgg gggctgagta tgccttacca gcaggttata
      481 gtgagcataa ttcaggttta tcactagatg taggatcaag cttgacgaaa atggaacgag
      541 cccctgaagg aaagtggata gaagaaaatg cttggaaata cgggttcatt ttacgttatc
      601 cagaggacaa aacagagtta acaggaattc aatatgaacc atggcatatt cgctatgttg
      661 gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt tctcgaggaa tatatggatt
      721 acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg ggaaaaatat gagatctttt
      781 attatcctgt tactaaaaat accaccattc atgtgccgac taatcttcgt tatgagatat
      841 caggaaacaa tatagacggt gtaattgtga cagtgtttcc cggatcaaca catactaatt
      901 caaggaggta aggatggcgg aatgaaacca acgaaattaa tgaacagcat tattgtacta
      961 gcacttttgg ggtaacgtta gctttttaat ttaaaaccca cgttaactag gacattgcta
     1021 tactaatgat acaacttaaa caaaagaatt agaggaaatt atattgggaa aaatattatc
     1081 tagaggattg ctagctttat atttagtgac actaatctgg ttagtgttat tcaaattaca
     1141 atacaatatt ttatcagtat ttaattatca tcaaagaagt cttaacttga ctccatttac
     1201 tgctactggg aatttcagag agatgataga taatgttata atctttattc catttggctt
     1261 gcttttgaat gtcaatttta aagaaatcgg atttttacct aagtttgctt ttgtactggt
     1321 tttaagtctt acttttgaaa taattcaatt tatcttcgct attggagcga cagacataac
     1381 agatgtaatt acaaatactg ttggaggctt tcttggactg aaattatatg gtttaagcaa
     1441 taagcatatg aatcaaaaaa aattagacag agttattatt tttgtaggta tacttttgct
     1501 cgtattattg ctcgtttacc gtacccattt aagaataaat tacgtgtaag atgtctaaat
     1561 caagcaatct gatctttcat acacataaag atattgaatg aattggatta gatggaaaac
     1621 gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg aaaaccggtg ataaagtaaa
     1681 aagcttacct aacactatag taacaaagaa agcccaatta tcaattttag tgctgaggaa
     1741 ttggtctctt taataaattt ccttaacgtt gtaaatccgc attttcctga cggtaccccc
     1801 aaactaagat ttcatagata gattttctga taactttaaa aaacacgaat agcggaaagg
     1861 atgtcttcat tcgctaaaga gtaataaact acttttccat cttgacgtga ttttgccatt
     1921 ccatgttttt ttaaaaaacg taaatggtgt gacgttgtag cgacactagc agtaagtatt
     1981 tctgctaaat cacagacgca catttcctta taggtttcta aagcatagaa aattttaatt
     2041 ctagaaaaat cgctgaaaca tttccctaac acaagtaagt ttgaaaaatc tttttgatcc
     2101 aatttatttt ttattccatt tactttttct ttatgtacga cagtcacttg gcaaactttt
     2161 ttcattcatc tcacctcaca ttcaaacgat tatttgaatg aagtatacct atttgatcac
     2221 tcttttgtca atctacctta attccaatac actattataa gcaatttcat gtattgaagt
     2281 aaacttactt gcaagtgcgt ttttgattcc aagttaaaaa catattatta ttctacataa
     2341 tttcctaatc aaataaaact cctttgaaaa ttgttctaaa ggagttttat tattttgtat
     2401 ttctactaaa aatagcgtac aatgtaatta ctaagtaatt acgaaatgta aaataatgga
     2461 ggaaataata tgattacaaa aacaagaaaa caaggaaatt ctattatgtt aacggtccct
     2521 aaagaattcg atgtgcccaa tggtgttgaa gtagaagcta agcttgtaga gaatggtatc
     2581 ctttatgagt ttgtagagcc taaaaaagaa ttttttgatt ttagtgaaga tattttggct
     2641 gatattcttt cagaaggtta tagcaaacaa gagatcttaa aagaatttag aaatagaaaa
     2701 aatgcgttaa cttctgcatt taaatctatt gctgaagata ctgttgcaaa ttctaaaccg
     2761 atgacaaaag aggagttggc agcagaaatt ggcttatgag attctacctt ctaaacacgt
     2821 aataaagtat ctaaaaaaat taaaagagaa aacattaaaa gaacaatttt taacgataat
     2881 ttatgatgag atagcagttc gtcctcattc gggagaacaa aaaacgggtg atttatcagg
     2941 aatttgggct attggattca agtatgcagg tacgacatat cgcgttgcat atgaaattaa
     3001 agataatgca gtgataccta tcttactatg tggaacgcat gaaaattttt atgaacaatt
     3061 aaaaaagatt aggtaagaac ccaatgtttc aacttgagag gatagacgac actttaaaag
     3121 ataattttta acagctgaga gttgtaatac cctataattt taaaccacta acctatatat
     3181 ttatgaaaaa tagaaaaaat taaaacgcat tttaagcctc tttcaaatta aaagcttcat
     3241 ccttatgcac aaagagggct taaaatgcgt tattttatat tgttttttag taataataaa
     3301 aaaatcactc taattctccc ataaatatta tagattccta aagattaaaa tagattattc
     3361 cctattgtct tttaagtagc ggtagagtgt tgccttgcta ataccagtca tttccgtaat
     3421 ttcttttacg ctataaacct gacttttata caattttact gctcgatcaa catctttctg
     3481 gttcactcgt ggacggccac ctttacgacc tctcgcacgt gcacttttca aaccttcttt
     3541 ggtacgttca acaattaaat cacgttcgaa ctgactaaat gcttggaaga cagtcatcat
     3601 taagcgtccg tgaggcgtgc tggtatcgaa gttttctttc aagctcacca gcttaacatg
     3661 gtgttcttca aaataggtaa caaggtcaat caaatccttt gtactacgac ccaatcgaga
     3721 aaaactttct acaattaccg tatctcccgg acgaatttta tcttttaaac gatttagttg
     3781 gggacgaacc gctttggttc ccgtcatttt ctcagttagg atttcattac aattttgttc
     3841 attgagtaag tctaattgac gatttagttc ttgtgaacgt gtactcactc gggcataacc
     3901 ataaatgtat ccactcatgc ttttcttcct ttcgtacttt aattctggct tgattgtatc
     3961 ataaaaggga gttatagata catagttaat gcaacaagtt atgatacaaa atatgacctc
     4021 ttttatttaa taaaagaggt catttcattt tgtctcataa tcgattgttt ttgatacgag
     4081 tgtgtatacg aaataaacaa aaaaatctta agtaaatttc tagataccta atttaatcct
     4141 aactagtgta tcctaaagtt aataacgagt ttatcacaat gaaaattata aaatgctccc
     4201 atattagata taacatgcaa tatttgaatt agaactacaa aatttattcg ttttataaag
     4261 taaataatgt tgagatacta aggaatagaa tattataagg aattgagctg aatgtattgg
     4321 ataaaaaaag aataacttta ttagagatgg actataaaaa tgctcgtaat tttcttctga
     4381 agtctgaaag ctatttgaat gctcagctac cagaatatat tagttttgac gaaactttaa
     4441 aatctgctaa aaaagtttta a
//
LOCUS       DACEMK010000134         4430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEMK010000134 DACEMK010000000
VERSION     DACEMK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.140"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(543..2048)
                     /locus_tag="IW715_002486"
     CDS             complement(543..2048)
                     /locus_tag="IW715_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1023065.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IW715_002487"
     CDS             complement(2045..2731)
                     /locus_tag="IW715_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1023066.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW715_002488"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IW715_002488"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1023067.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DACEMK010000135         4391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEMK010000135 DACEMK010000000
VERSION     DACEMK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.141"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            708..2567
                     /gene="ltrA"
                     /locus_tag="IW715_002489"
     CDS             708..2567
                     /gene="ltrA"
                     /locus_tag="IW715_002489"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1023068.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
     gene            2688..2945
                     /locus_tag="IW715_002490"
                     /pseudo
     CDS             2688..2945
                     /locus_tag="IW715_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2948..3101
                     /locus_tag="IW715_002491"
                     /pseudo
     CDS             2948..3101
                     /locus_tag="IW715_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            3124..4329
                     /locus_tag="IW715_002492"
     CDS             3124..4329
                     /locus_tag="IW715_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1023069.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1584 a          750 c          893 g         1164 t
ORIGIN      
        1 ggttaggcac ttaaaagaag aaaacataag tttcctttta aggagaactg atattttgag
       61 cagtggaatg atggggtaac gccctgaaac gctcccttaa tactccgact ggctgtttaa
      121 catatagctg ttagatggtc agaagctcgg tgaagtcggc tgaaagttac cgtaaatatg
      181 ataaattcat atacgaaagt tgttcagagg tgaatgatgt aacctatagg tcggaggtct
      241 ataaaatact cacggtgaga atgtaagtaa tactactgac gaacttgcga atgaaagaat
      301 ctatacacgg actgtataga aatgtacagg attctaccgt atcaacggaa cagaatgtat
      361 gttgtggttt agtaagatta ttggttatga acaaccatgc aatgttacag gcattggaaa
      421 tatacaggtt caaagcaagc acctaaaagt atatataaag ataaaaatat cgaaacatgg
      481 gaagctggca atgtggagga aactatattc cattgaaaca tttgtgacga agaataataa
      541 gcacatttat gccagtgaaa gtggtggcac agtaccaatg aggatataga aatatatcgg
      601 aggaatagcc actagtcaaa agaaatcgtt aattaaatga tgtatttcat agaatcgagt
      661 aagacaaaat aaacactaca acctaagtaa acaaaggtag tggtgctatg aataaagtaa
      721 aaacaaataa aacacttcgt catagtgaat actatggcat gacacaaata tttgatgaac
      781 tgtatgtaaa aagtgataaa aaagaagttt ttacagagct catgacaatt attatgagtg
      841 atgaaaatat tcgactagca tacagaaata tcaagaataa caagggcagt tatacagcga
      901 gtaccgataa gaaaagtatt cgagacatta aaacactgtc agaagatacc tttctaactg
      961 aggttaaagt tctttttcgc tgttacaaac cacgagcagt tagacgtgta gaaattccca
     1021 aaccaaatgg aaaaacacgc cctctgggca ttccatcaat ctgggatagg ttaatacaac
     1081 agtgtatttt acaagtctta gaaccgatat gcgaagctaa atttcacaat tatagttatg
     1141 gatttcgacc aaaccgttcc acacatcatg cgattgctac ctgcaatcga ctggcaaata
     1201 gggggaagct acactttgta gtagatgttg atattaaagg attctttgat aatgtcaatc
     1261 actctaaact tttgcgtcaa atgtggacga ttggaatccg tgataaatgg ctattaggta
     1321 ttattcgatc catgctaaat gctcctatag tcctaccaac cggtaaagtc gaacacccta
     1381 aaaaaggtgt acctcaaggt ggtgtcctat cccctttact atctaatatc atattgaatg
     1441 aattagattg gtgggttgct tcacaatggc aaaataccac gattgagaca gaaaagatta
     1501 tggataggcg agataaaggc aaaggcattg ataagactaa tcagtatcgg gcattaagac
     1561 gaacaaactt aaaagaaata tacattgttc ggtatgctga cgacttcaaa atcttttgta
     1621 gaaaacgaag tcatgcagat aaaatatttc acgcaacgaa aatgtggctt aaagaacggt
     1681 tatcacttga aattagcgaa gaaaaatcca aaattgtcaa tctcaaaaaa ggtaaatcag
     1741 aatatctagg atttgaatta aagctaatca agaaaggtaa aaaatacgta gtcgaaagtc
     1801 atatgagcaa aaaatcaatg acaagagtta aaatccaatt aaagaaacaa ttacgtaaag
     1861 ttgccagacc gaagaatcat tgcgaacaag caaaagaaat tggattatat aattctatgg
     1921 tgattggaat ccatcaatat tatggaattg caacctgtgt aaatctcgat tgcagtaaaa
     1981 tagcctatac actaaaaccg ttcatcaatc acaaactgcc gacaaagaaa catggaaaga
     2041 tattaaatac atttatcaaa tcgaagtatg gaaaaagtaa agaaatgcga tggttgaata
     2101 atgtgccaat tgtaccgctc aactatgttc agtttagact tgctgtcccc ctatcagagg
     2161 gaacttgcaa gtacactgaa agtggacgaa gcaagataca cagtaaatta aaattagacc
     2221 tagccctttt acttcacatg atgagaaata gtaattatga aagaagtatt gaatttgtgg
     2281 ataacagaat atctaaatat tctgctcaaa agggaaaatg tgctatcacg ggtaaatttc
     2341 tcgaatacga agaaatccat tgccaccata tcgtaccagt aaaacaacag ggtacagaca
     2401 aatacagcaa cctcattatt attcacaaaa atgttcacgc attagtccat gctatcgaag
     2461 aaaaaatcat acacaaatac ctgaatgtat taaatctaac caatgaacaa atagaaaaac
     2521 taaatgaatt acgtgtcaaa gctggtaata gtgttttaac agtttaactt gaaaaatata
     2581 tcatacttaa cattcttttg atggaacgcc gtatgaagtg aaagtttcat gtacggtgtg
     2641 aagcggggga aaatctggag atgacatcaa aggattacct atcgctattt aatttcagag
     2701 tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt gacgtacaaa
     2761 aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga acaagtgtca
     2821 tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag gaaattaaaa
     2881 agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc gcaggtgtgg
     2941 actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct cggtatctaa
     3001 caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca
     3061 agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca tacgcctttt
     3121 tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa agaaaaacgc
     3181 ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac aaggcagtat
     3241 ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca gtccctttgg
     3301 gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga ttatgtaaga
     3361 attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca actgaaactg
     3421 tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta tgctttaggc
     3481 gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga attgaaaggt
     3541 cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg gtatgagttc
     3601 tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat
     3661 aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga agaatgtatc
     3721 tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa agaggaaaag
     3781 gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta tttctgtatc
     3841 tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga cgcagaagta
     3901 aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt ccgtgattta
     3961 ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta tatccgtttt
     4021 gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga atgggcttgg
     4081 tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc ttactccttc
     4141 caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt tgcgattaaa
     4201 cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc gaaactgaca
     4261 gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt gataacaaca
     4321 aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt tatgaatttt
     4381 ggacaaaacc t
//
LOCUS       DACEMK010000136         4381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEMK010000136 DACEMK010000000
VERSION     DACEMK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.142"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            149..1072
                     /locus_tag="IW715_002493"
     CDS             149..1072
                     /locus_tag="IW715_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1023070.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1090..2331
                     /locus_tag="IW715_002494"
     CDS             1090..2331
                     /locus_tag="IW715_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1023071.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="IW715_002495"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="IW715_002495"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1023072.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1509 a          694 c          943 g         1235 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     4321 tttccatttg tttctctaat actcaggaca aaacactgat tttacaacct ctgataaacg
     4381 g
//
LOCUS       DACEMK010000137         4374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEMK010000137 DACEMK010000000
VERSION     DACEMK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.143"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(83..1051)
                     /locus_tag="IW715_002496"
     CDS             complement(83..1051)
                     /locus_tag="IW715_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1023073.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IW715_002497"
     CDS             complement(1498..2904)
                     /locus_tag="IW715_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1023074.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IW715_002498"
     CDS             complement(3126..3488)
                     /locus_tag="IW715_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023075.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IW715_002499"
     CDS             complement(3492..3800)
                     /locus_tag="IW715_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023076.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IW715_002500"
     CDS             complement(3809..4228)
                     /locus_tag="IW715_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023077.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IW715_002501"
     CDS             complement(4221..4361)
                     /locus_tag="IW715_002501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023078.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACEMK010000138         4274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEMK010000138 DACEMK010000000
VERSION     DACEMK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.144"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..530)
                     /locus_tag="IW715_002502"
     CDS             complement(27..530)
                     /locus_tag="IW715_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1023079.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(653..1417)
                     /locus_tag="IW715_002503"
     CDS             complement(653..1417)
                     /locus_tag="IW715_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1023080.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1525..1800
                     /locus_tag="IW715_002504"
     CDS             1525..1800
                     /locus_tag="IW715_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1023081.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2238..3188
                     /gene="yidC"
                     /locus_tag="IW715_002505"
     CDS             2238..3188
                     /gene="yidC"
                     /locus_tag="IW715_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1023082.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(3367..3567)
                     /locus_tag="IW715_002506"
     CDS             complement(3367..3567)
                     /locus_tag="IW715_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023083.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         1259 a          908 c          752 g         1355 t
ORIGIN      
        1 atatggtcta ttattggttt tatctattaa atcgaggatt ctttcccaaa gtaacgttgc
       61 catctcttac gcatacttgg tgacataggc tgagcggctt ctttgactgc gcagtatttg
      121 tctacgaatg ttacgatata tccttctttg tacttaggcg gtgcaatcgt tgcgccccac
      181 atgtgcttca aaatgatatc tcgctcaaga tccgacaatt cggtgatctt ttctgcattc
      241 tttacagcaa ttcttggatg gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc
      301 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
      361 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
      421 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
      481 tcttctacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
      541 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
      601 ggattattct agtcgccttg gataaaatag ccaaagctta atagttcctt aatcataaag
      661 aaaaatgata aagaacaatc cctgccgcaa ccccaacatt caacgattcc gcatttcctt
      721 tcatcgggat atatacgttc tgatcagtca aagacaagat ttcctgactt accccctgac
      781 cttcgttccc catgattatt gcgaagttat ctgatttttc tactttgtct aatgcgtgtg
      841 cttcttcatt taattctgtg ccatacactg taaacccttt gtctttcatt aaaggaatca
      901 cttctgtcaa atctttttgc aacacaggca aatgataatt gcttccctgc atgctgcgca
      961 gcacctttgt actataaata tctgccgttc ctttgcctaa taaaacacca gccatatcaa
     1021 atgcatctgc cgtacggatc atcgtcccta catttccagg atcttgtaca ttatctaaca
     1081 acagccatcc tcctgaaaaa tctggcgtgc cttcagccgt taacgccatc actgctaaga
     1141 ttccttgtgg tgtaggtaga tccgatacag catccatgac ctcatccgaa actagaaaag
     1201 tagaaatctc tttgtttgcg gccagccagt ctccccattc tttcatgcct cgctgtgtca
     1261 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcaatcaga tggaaccctt
     1321 ccaaaagata ttcttccttt tcttcccgat atttcttttt atgtagtttt tttaattctt
     1381 tgattcttgg attttttgct gattggatct ctttcacatt cctcacctcc ccatgattat
     1441 atcatggaat aggtgcttga gcttgtttca tcctaaaaaa atcggtatca ttaatttata
     1501 aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc
     1561 caaggcgtag gatttcgcta catgaccaag atggtcgctg atcaactagg cgtgactgga
     1621 acggtaaaaa acgaagatga cggctcagtc acgatcgaag caatcggcaa tgacgacatc
     1681 atccagaaat ttattgaaga agtcaagaaa tctccgagcc caagtggacg agtgcagtat
     1741 gtggatgtcc aagaagaccc gatgattgag gaacgaaaga aatttgatgt ggtgggataa
     1801 ggttgtgaaa aagctggtct gcatcaagca ttaaggacag gttgtgaaaa atagctctcc
     1861 atatttttca acaggctgga cttaatgccg tagatgcaag cttttgaaca ccgtcgataa
     1921 ggttattctt gaggcgcttt gcctcaaaaa ttaaggacag cagtgaaaaa gctgatctat
     1981 cacttgcagt gagttcattt gactccgctt tttcgggcag aaaaaaagtt tttagtttat
     2041 taaagtaaga aagaaacact tggaatcatt ctactttttc ctagtatttc tttcttgatt
     2101 ttttgtcttt ttttcaaaat ttttcgctac gtaaaagaac gaatatttat atgaaatttc
     2161 tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat
     2221 agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca
     2281 gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt
     2341 cgggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg
     2401 aaaatttcaa ttggtcttat ggctgggcag ttattgcaat gactgtgatc gtccgtatca
     2461 tcatcttgcc actaggtatt agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc
     2521 aagcgttaaa accacaagtt gaagcagccc aacaaaaatt gaaagctgca acaacacgtg
     2581 aagaacaaat ggcagcacaa gcagaaatgc agcaagtata ccgcgaaaac ggattgagta
     2641 tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt
     2701 actttacggc acgttataca gaaggtatcc gggaatcatc tttctacggc atcgatttag
     2761 gctcaccaag tatggtactt gttgccattg caggggtcgc ttatctgctt caaggataca
     2821 tctcaacgat cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct
     2881 ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca ctttactggg
     2941 tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg atgaaaccac
     3001 gtttgaaagc acaagtggca gaagagttga aacaaaaccc accaaaacaa gtcgtcactc
     3061 caagaattga tgtgacagaa tcagctaaac cagctgacag caaacaagca accaaacaaa
     3121 tcaagcaacc caaaaacagt acaaacaata aaggccgcaa cgcaggaaag cagcagaagc
     3181 ggaagtagat agtaaataag agtgtaataa aaattcatta acagttttag caattaaaaa
     3241 tatcgaccaa gatttttgaa aaagaaatct ggtcgatatt tttatttaag tgaatgttta
     3301 atttgaatca tcgattgatc gctatctaat tgcagcagca tctttttatg aagaaacaac
     3361 tttttcttat ctatcaacgt agacaggtgt tgctgtttct tctaatagtg tacttaattc
     3421 cttaatattt tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt
     3481 aacaaaaggc gccactccct tcgcccatgt tgctcgccca cataagacac cattctccat
     3541 aaaggacaac gcggagatac cacccatcta aataacatcc ataaaccttt ctagtagaag
     3601 aatagtgata gtatccatcc ctttttctac tgggaataca attacacgat tcaaagcttg
     3661 atacacaaca tttcccttct tataaaccgc tattgtaacc gaattgtttt gaatcacctt
     3721 tttgggatag aaactggctc ctttccgcaa cttcttaaat gttggtttct tttatacttt
     3781 ctttgtagca actacaaatt tcttcaaatc tctaaatgtt tgcataacaa ccgcctttct
     3841 gacttttact caggatggta tttaaaaatc agcaaaataa ggcttctctc catctttttt
     3901 atttcctagc tgctttaaag acactcttgc tgttttttaa tctctatcaa gggggctctg
     3961 aatctatttt gatgaactta tcttattaat ttctcaaatg agctgtcaga cacaactgta
     4021 ttttttcaat ctcataccgg acaactatgc cagcttaaaa aaatttcctc tggcaaaact
     4081 tctttattga aaaatgacaa tgaaaaaaga ccattctttt tgttgaatgc tcattgtgta
     4141 aacatatttc ctgcgactgc tcttcgaacc attctcagtg caatccctaa tttatctatt
     4201 aaatcttctt ttagttcctc atctttcata ttcaaaattt cctttgtatg ttacacaagt
     4261 ttgttctcta aata
//
LOCUS       DACEMK010000139         4266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEMK010000139 DACEMK010000000
VERSION     DACEMK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.145"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1485
                     /locus_tag="IW715_002507"
     CDS             <1..1485
                     /locus_tag="IW715_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1023084.1"
                     /translation="CEAKFSENSYGFRPNRSVENAIKATYNRLQISQLHYVIEFDIKG
                     FFDNVNHSKLIKQIWAMGIRDKHLIFILKRILKAPIKMTNGTITYPEKGTPQGGIISP
                     LLANIVLNELDHWVESQWQENPVTKNYVVHINKSGSPCKSNAYKEMKKTKLKEMYMVR
                     YADDFRVFCRYKESAEKAKIAITQWIEQRLKLEVSQEKTRIVNVRKRYSDFLGFKIKM
                     IPRRKKLVVKSHISDKQFKNQKDKLVSQAKKIASPSKGKTELEETRLYNSMVLGMQNY
                     YQIATNVNLDCMKLNRAVMAIFTNRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYV
                     AGEGEPIFPVGYIQHRKPMAKVYLANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENR
                     TIEYADNRISLFSAQHGKCAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAV
                     HRLIHAKTEETIQKYIALLKLNKVQLIRLNKYRVLAGNQELNLI"
     gene            1589..1813
                     /locus_tag="IW715_002508"
     CDS             1589..1813
                     /locus_tag="IW715_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023085.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            1826..2329
                     /locus_tag="IW715_002509"
     CDS             1826..2329
                     /locus_tag="IW715_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1023086.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            2389..2778
                     /locus_tag="IW715_002510"
     CDS             2389..2778
                     /locus_tag="IW715_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1023087.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            2765..>4266
                     /locus_tag="IW715_002511"
     CDS             2765..>4266
                     /locus_tag="IW715_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1023088.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT         1427 a          696 c          919 g         1224 t
ORIGIN      
        1 tgcgaagcta aatttagtga aaacagctat ggttttagac ctaataggtc agttgaaaat
       61 gcaataaaag caacctataa ccgcctacaa atcagccagc tacattatgt gattgaattt
      121 gatattaagg gtttctttga caacgtgaac cattccaagc tgataaaaca gatatgggct
      181 atgggaatac gggacaagca cttgatattc attttaaaaa ggatattaaa agctccaatc
      241 aaaatgacta acggtacaat aacatatcca gagaaaggaa ctccgcaagg gggaataata
      301 tccccactac tagcaaacat tgttcttaat gagttagacc attgggtaga aagccaatgg
      361 caggaaaatc ctgttacaaa aaactatgtg gttcatatca ataaaagcgg tagcccatgt
      421 aaaagcaacg cttacaaaga aatgaagaaa acaaagttaa aggaaatgta tatggttcgt
      481 tatgcagatg attttagagt tttctgccgc tacaaggaaa gtgctgaaaa agccaaaata
      541 gcaatcactc aatggattga acaacgatta aaattggaag tatcacaaga aaaaacaaga
      601 attgtaaatg ttagaaaaag atactccgac tttcttggtt tcaagattaa aatgataccg
      661 agaagaaaga agttagttgt taaatcacat ataagtgata aacaattcaa aaaccaaaaa
      721 gacaagctgg tatcacaggc aaagaaaatt gcttctccct caaaagggaa aacggaatta
      781 gaagaaacaa gactttataa ttcaatggta ttaggtatgc aaaattatta tcagattgca
      841 acgaatgtaa atctggattg tatgaaactg aatagagctg taatggctat cttcactaac
      901 agattaggta caaacaaaag gggcagattg agaaaaacag gcagacaact tacgacaaag
      961 gaggcaacaa gatatggtaa atctaaaatg cttcggtatg ttgctggcga gggcgaacct
     1021 attttccctg ttggatatat ccaacacaga aaaccaatgg caaaggtgta tttagcgaat
     1081 tgctacactc tagagggaag aaaatttatc cacaccaatc taagtgttga taaattactg
     1141 atgtaccagt taatgaaatt atctttggaa aatcgcacaa tagaatacgc agacaatcga
     1201 atttcgctat tttctgctca acatggaaaa tgtgctataa ctctggaaga atttcaacaa
     1261 gctaatgaaa tacattgtca tcatattgta ccaactggag ctggtggaaa tgacgattat
     1321 aaaaatctta ttttggtaaa agaggcggta caccgtctaa tacacgctaa aacagaagaa
     1381 acaatacaaa aatatatagc tctattgaag ctaaataaag tccagctcat aagactaaat
     1441 aaatatagag tattagctgg caatcaagaa ttaaacttga tataggtcta ctacataaat
     1501 acttttcaat ataaacacga tggaacgcgg agtgcggtga aagtcgcacg ctccgtgtga
     1561 atggggggaa aagtttgaga tatttataat atcaggaact tacctatcca tatatcaatg
     1621 gtttttaacc aatgcccaat ccttagtctt actggcaatt gtggtcattg gtttgttttt
     1681 aggatttaag cgggagtttt ccaaactgat tggctttttg gtgattgctt tgattgcggt
     1741 cggattggtc tttaatgctt ctggggtgaa ggatgtctta ctcagtttgt ttaaccgaat
     1801 tgtcggtgcg taagggggaa gcacgatgga acagatgcgt gtttatattg cgaacctcgg
     1861 gaaatataac gaaggtgaat tggtgggggc gtggtttacc ccacctgtcg attttgatga
     1921 agtgaaagaa cgaattgggt taaatgacga atacgaggaa tatgcaattc atgattatga
     1981 acttcccttt gagattgatg agtatacgcc aattgaggaa attaatcggt tgtgtggctt
     2041 agcggaggaa cttgaaggaa cgccagttgg tgaagttgct tctgagattc aacatgcttt
     2101 cttcaattct tttgaggaga tggtagagca tgtggatgat attatctatt atccagattg
     2161 cgatgatatg agtgatgtag catatttctt gattgtagaa gctggtgatt ttggagaagt
     2221 accagaacac ttgaagacgt atattgattt tgatgcgtgt ggtagagact tggagattgg
     2281 aggcaattat ttggtaacga gtcgaggtgt ttttgagtat ccatattaac aaaattattg
     2341 aagggtagtt gatttctaaa ctatccttct ttttaaagga atgatttgat gaagaaaata
     2401 aaaagctaca ccagtatttg ggcagttgaa aaggtcattt atgcaattaa tgactttcaa
     2461 ctgccttttc ctgtgacttt taaccagatg gcttggtttg ttttatcctt gctgtttgtc
     2521 atcgtatttg cccatgtgcc tccactatct atgatagagg gggccttttt aaaatactta
     2581 ggtataccgg tggcagttac ttggttcatg tcgcaaaaaa cgtttgacgg taagaaacca
     2641 ttgggatttt tacgatcttt tattagctat cacttacgat ttaaggtcac tttcgcagga
     2701 aagaaagtca aagaacagaa gaaacgatgg gacgaaccca ttactctggt aaggagtgtg
     2761 aactatgtac ccgattaagt atattgaaaa taatcttgtc tttaatcaag agggagaatg
     2821 ttttgcctac tatgagttaa taccttacaa ttactccttt ttgtcacctg aacagaaata
     2881 tcaagtccac gataactttc gtcaattgat tgcgcaaaat cgtgaaggca agattcatgc
     2941 cttacaaatt gccacagaaa gtagtattcg tgccacgcaa gaacgatcaa aaaaggaaat
     3001 taccgggcga ttaacagaag tagcgaaaca acgaattgat ttacagacag aggcattagt
     3061 atccatgatt ggcgatagtc agattgatta tcgctttttt attggcttca aactgattgc
     3121 gacagatgaa gaggtcaatc taaagaatct caagaaatct ttcttctccg gctttcaaga
     3181 atttgtctat ggagtgaatc atcatttgat gggcgatttt gtttccttat ccaatgaaga
     3241 aattcgccga tataccaagc ttgaaaaact aatggaaagt aaattggctc gaagatttaa
     3301 agtcagacga gtcacaccga gtgatctgat gtatttgatt gaacatatct atggcgaaaa
     3361 gggtacacca tttgaagaat atgaatttca gctaccaaag aaaaagctga agtcggaaac
     3421 tttggtgaaa cgttacgact tattacgtcc cagtcgttgt ttgattgaag aaaagccccg
     3481 gtacttatgc atggaacatg agaatcatga atcatatgta gcgtacttaa caattaatac
     3541 aattgtgggg gaaatggagt ttccctcctc agaacttttc tattaccagc aacaacaatt
     3601 tacattccca attgacacta gtatgaatgt ggagattgtg acgaacaaaa aggcacttgc
     3661 taccgttcgc aataagaaaa aggaattaaa agacttggac aaccatgcct atcaatctga
     3721 caatgaaaca aattccaatg tcttagatgc attggattcc gtagatgaat tggaaactac
     3781 cttggatcag tccaaagaat ccatgtacaa actgagctat gtggttcgag taagtgccga
     3841 atcagtagat gaactaaagc gccggtgtga tgaagtgcta gatttttatg atgataccaa
     3901 tgtaaaacta gtccggcctt ttggcgatat gatggggcta catgaagaat tcttaccttc
     3961 tagtaagcga tacatgaatg attatatcca gtatgttacg tctgattttc tcgccggact
     4021 tggttttggc gccactcaga tgttagggga gttggaaggc atttactttg gttataacgt
     4081 agatacgggg cgcaatgttt acctgaaacc ggcattggcc tcacaagggg tcaagggttc
     4141 agttaccaat gcgctagcag cggcattcct cggttcactc ggtggcggaa agtcctttag
     4201 taacaatctc ttagtttatt acgccgtcct ttttggtgga caagctgtga ttgtcgatcc
     4261 aaaagg
//
LOCUS       DACEMK010000140         4253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEMK010000140 DACEMK010000000
VERSION     DACEMK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.146"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            689..1501
                     /locus_tag="IW715_002512"
     CDS             689..1501
                     /locus_tag="IW715_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023089.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3136..3690
                     /locus_tag="IW715_002513"
     CDS             3136..3690
                     /locus_tag="IW715_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1023090.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3944..>4253
                     /locus_tag="IW715_002514"
     CDS             3944..>4253
                     /locus_tag="IW715_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023091.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         1564 a          528 c          678 g         1483 t
ORIGIN      
        1 atgtacttgg gtcatgtaaa agtcctcctg ggtatgtttt tgtcgttaaa aacattgtac
       61 cgtaaaagga ctgttacatg gcctttttac ttttacacaa ttataaggac tttatcgaaa
      121 aaaagcagtt atttgttaaa atcattgata ttattaataa taagtatttt ttgaaagggg
      181 aaatataaaa ggtttttgaa aataaataaa aaatagtatt acttgttcta ttgctagtta
      241 ttgctagtct ttattctttt ttgtttttta gggatgtaca aaagatctca accacaggag
      301 acattgcttt tcacttttct agaataaaat gcttgtcctc agtattttct ggtccaataa
      361 attttaccac atttaatagc tatggcggag gcgtgaattt ttttatccat atctaacatc
      421 tttccagccg taatatttta ctggacaaca aacaatttga ttctaagtta tatacttact
      481 atacgttggg atactgaata tctgtacaat tctagtgagc tatcaatatg gacaaaagaa
      541 tagatgcagc cttcttattc agctgtttgt atagctttta tggattgata tatattagcg
      601 tactgcaatt tcagaatcaa ttgcgttgac gattgtacca gttgtcttgt attacacata
      661 tactcttctt tatgaaagaa aaaactgcgt gattctttta gctgtcagta tgagtttgct
      721 catctatacc catattctct ccacctttat gagtgtgatt gttattggct tcttgtttat
      781 cggctctctt ttctctaagc ggaaagagag aaaagaaatg tcgaatctga tatcaaatat
      841 gataaaagct gttggttgga ccattctttt aacagcttac gtttggtatc cgatgttaca
      901 acaagtccta catcaaaaaa ttaatcgtcc ttttcgcaca aaacttcaag aaagggcatt
      961 aaacatttct gatagtttaa tcggtgcagt aaataatgat ttagttacat ttacaatggg
     1021 aacaattggt gtggtttcat ttattcttcc acttttcttt aaaaaaaaat ttgccaataa
     1081 agaaaagatt attaattttt gtgcagtgac cacttggttc ttatcaacga atctttttcc
     1141 atgggaattt ttacaaaaaa cacctattca aatcattcag tttccttgga gaatcttggg
     1201 ttttcaagtg ctttttggtt cattgatctt aattatagtg tttttaaagt ggaaaacaag
     1261 caacaaaaaa agtatgtgta gccttgtagg aatagtcctt cttattttta cagtgactgt
     1321 agcaacagaa gcgaattatt cgcagaaaat tcaatcgtat aaaggtcgtt taattatgac
     1381 caaaaaagat gttacacttt atacaacctc gagaactgga ggtctttatg actatgcacc
     1441 attagatgca ttgaaatata aagatcattt aaagaaacat gaagtaaaag tctatgattg
     1501 atgggagaat gcatctcata aagcacataa ttctaaaata atctatacag taggagatgc
     1561 gaatgagcaa aatatcacgt tacctgtatt taattactgg gaacagttat gaaagtaaat
     1621 gggaaaaaga gcactataga aaatgacaat ggtttagtgt ctattaaagg tacgggaaaa
     1681 agaactatag ttgaggtttc atctacgtat ccaactacga tgattctatc tttattaatt
     1741 agttcaataa cttttatatt cttatttaca cgttatttaa taagatgctg cggttaaatg
     1801 aaaatagatt agatagatat tataaatatg ttgattctat tttcatagca atggagaaaa
     1861 tacattagca atatataaaa gaaggtatca agagtcacgg tgaatttatt ccgaaaagtg
     1921 aaaagaacaa atataaaatt taataaaaat atagaactaa ataggaaaac gtagacgtaa
     1981 ttttctacgt tttccttttc atatatatta aatattacgg aaatttatat aaactaattt
     2041 agtgttttgt ttgtaattcc taattttgct ccacttttag ataattttta ggtctattta
     2101 actcatcaga cattctatgc tcattttctc caaatttgtt tcaactcttt cgtacttaat
     2161 tttgcgtatt tgaaaattct tcttttagag tacttttatt acgatactag tatgatatat
     2221 atctatagca aacagttaaa atcttcctaa aataagcgaa tagcattcct aaaacctata
     2281 aaatttttag atgaagcgac aagtggacaa ttaataaact acttgaacat gaaattatgg
     2341 atcacctttc aaatttagaa aatggaattc ttatacttat tgcgcaacat ttattgattg
     2401 caaaattatg tgacaaaaag aactagtaga agaaggaata atggcttgat aataaagtac
     2461 tacctattaa aggtagtact ttatttaatt tagtataaat taacatacta ctatatttta
     2521 agtttcatat tatataaatt aataatatac taccattttt tggtagtata atttaaaggt
     2581 aataagttat tcttttatta ctatgtttgg gttttatagc aaggaaagtt taataagagt
     2641 ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta gtgatataac
     2701 gaaatatcta cacagacaaa attatgatct tgcgtaaagt tataaaatat taataaaaat
     2761 attttattaa tgaaaagctt ggtagcacgg ttattcatat gctaatttat ttcactttat
     2821 agtaagggga gttcttagta tattgtccaa atttaaattt catataattt aattatgtaa
     2881 attagctatt ctaagaactt ctacggataa ctgcccgtct aaaatttaat taagagctta
     2941 gaatcttcat ataagtaagg ttaatttttt tgtacaaaat agtcggaata ttctgagtta
     3001 ctgaaatgat ataaaatcat aaactaaacc ccttgatttt cttcactttt aacagagtat
     3061 atatataagt tcatgattat ggtaaaatat atatataatc atgaactcag aatattttta
     3121 agaagggaaa caatagtgag ctataatgta caaccgttaa ggacaaatga agagatacaa
     3181 gattttcagt tttggctaag aagaacaagt aatccagaaa gagatagttt tttatttttg
     3241 tttggaatca ataatgggtt acgtatgtca gatattatag gattaaaagt tggagacata
     3301 cgaggtaaaa gtaagcctat tattgttgaa cataaaacag gtaagcgaaa accgattttt
     3361 atagataatt tgagagaaga gattttatta tatactgaag gtaaagaaga aaatgattgg
     3421 ttatttccaa gtcggcaaca aggtcgacat ataacaaggg atcgtgtcta tcagatatat
     3481 gcagatatag cagaaaaatt aggaagagat gatataggaa cacatacatt aagaaaaact
     3541 tttgggtatc actactacaa gaaaactaga gacattgcta ccttaatgtt tatttttaat
     3601 catagctcgc aagcaattac aaaaaggtac attggtataa ctgaagatga aataggggca
     3661 tcgttacgtg gttttaaatt gggaggctaa ttgagatatt aggatatatt ttttgtgtat
     3721 aatgaaaact tagaaggtga gattaatgaa gataactgaa ttttcggaag tatttaaaac
     3781 gtttagtaac atttacaata gaataccaga gatagaacaa aatgttgtag ttgataatga
     3841 ttattatata gagaatgatt ttaataaacc aagaaaatat gacaacctat acaattttat
     3901 acacaataga acaacaataa ataacaacag taacactcga gttttgatta attcagcata
     3961 ttttcatatt aaatatttaa agaaatatga gtatgaagaa gttgtattag attatgggct
     4021 aaatagacag tcatttttaa tttatgcaat aaataacgga aatttgccaa gcaataaaaa
     4081 gaacataatt attgaaacat atgcctatct ggagggggga tctaaaaaac taattaagtc
     4141 atgtaatata gagtatcaat tatcagtagg agaaattaaa tgtgttttcg aagaagattt
     4201 agaacttgac ttaaaaaaaa tttttgccag cattcatgat tatataggtt tga
//
LOCUS       DACEMK010000141         4247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEMK010000141 DACEMK010000000
VERSION     DACEMK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.147"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..853
                     /locus_tag="IW715_002515"
     CDS             <1..853
                     /locus_tag="IW715_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1023092.1"
                     /translation="LTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIE
                     QIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIR
                     SEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYY
                     TKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHIT
                     IYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLV
                     DNFNNIS"
     gene            870..1304
                     /locus_tag="IW715_002516"
     CDS             870..1304
                     /locus_tag="IW715_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023093.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1921..>4247
                     /gene="esp"
                     /locus_tag="IW715_002517"
     CDS             1921..>4247
                     /gene="esp"
                     /locus_tag="IW715_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1023094.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1558 a          589 c          799 g         1301 t
ORIGIN      
        1 tttgactttg tattctttag gagacattcg agatattctg attttagttc attatttttt
       61 tactggaaaa atagaagatc tattccatga accgttaatt gaatatacag aaaatctaag
      121 taaaactatc gagcaaatca aaatagataa tctgttatca caaaattacg atccagaaat
      181 atatctgttt ctttatgaaa ataaaatact ggaatatgtt aaaaatggag atattcgaaa
      241 tttagaaaac atggttttta atctgagtaa tggaataatt ccaagtgtta gtggagatac
      301 gattcggtct gaaaaaaatt attctatcat tgtttttgaa aagttagcac aaacgtctat
      361 aacattagga atggatataa tagaagcgta tcaaagtaga gatgctttga tacaagaaaa
      421 cgaattagct gtatcgttac ctgaggtctt aaaagtgagg gattctggga ttgtttatta
      481 tactaaggaa atcggaaaaa ctaagataga gcatctttct cccttaattt cttctgtcgt
      541 tcaattcatc ggcttaaata tctataaaag aattactgta aaggaaatag ctaattattt
      601 ttctgtaagt gaaactaaat taagagaaag ctttaagaat gaaatgcata ttacgattta
      661 taattatatc tctaagcgaa aaatttctac ggctaaaata atgttgaaat caaaccatac
      721 tatcagtgaa gtttctttag gtttaggatt ttcagattct tcacactttt cgagagtatt
      781 caaaaagtac gcaggtgtat ctcctaagca atatcaatta ggtttagtgg acaatttcaa
      841 caacattagc tgagaggttt tattttatta tgagactata tcatttacgt agtttggagg
      901 agttgaggaa attggatttt aacaaaaaat ttttttggga aatttctgat ttgagttgtt
      961 tcgtagaatc tgagcgtcta agtaatggta ataatagtgt gttaaatgaa tatgacaaag
     1021 tagtaattaa taaagtaaaa agtttatgtg aagaagctag agaatctatc agtcatatta
     1081 atggtagcaa ttttaaacaa acattattta atctgattaa gctagatgct aaaatatcaa
     1141 gttatttatt ctttttgata catgatgaat ttatggatta tcaaaaattg gatcagttaa
     1201 ttgaaagaga aagctgggaa gattattacg tgggtctaac tttttcagaa aagttaaaca
     1261 actacaagtt aatagattat aagtatttga gtgaaagcaa ttaattattc atgtagagta
     1321 ctagacactt taaatatgga caattcaatt ataaagaaaa agaatcaata atttaatgag
     1381 gctgtgacaa atgtcatttt ttgtttcgct aaaaacgagt gaaaaaatgt tgataactcc
     1441 aatcattcgt ttaagattct aggaatcatt cagaaatgct gtgaaaccga tactttataa
     1501 ttataaagaa ggtgatttca aattccgaag aaggaatttg aagtcacctt tttacttatg
     1561 tcacagtctc attcagtaat gcgggataag gctccttatt ccgttatttg aatactctga
     1621 agtttaatct aagaaagggt aatattttga aaacatgctt catcgcatag aaatgaactt
     1681 aatttcagaa agaatacata catatctttt tatatgtttt cagctgctga atggttaaaa
     1741 agcacaaata tccgaaaaaa acatgaagaa atgtactttt tttgttgtaa tgttcatgaa
     1801 attctctaag taaattagtt aacataaggt tagtaagatt cctattctgt gaataatacg
     1861 tatcagattt atcttttatt cttactagga aagtaattat taagggagtt gatttgaata
     1921 atggttagca agaataataa gagagtattt ctagaaaaaa caaagaaacg agtattgaag
     1981 tacagtatca aaaaattaag tgttggggtt gcatctgttt tgattggtgt tggtcttgtc
     2041 ttgggaacaa ctgaactagt taaagcacaa gatgaaattt ctccaagcag cccacttgaa
     2101 acagcgataa gctccgtaca agtaggtgac aaagtagctt cggggaatac ttttcaggag
     2161 aatcctggtt atacgaaaaa ttataacttt tcggatttac aatttagtcc tcaagaacta
     2221 actggagaca cactaaaagg caatacaatt ggatttgaag tttacggaaa acataatatt
     2281 gcagcatcaa ctaaaaactg ggaaatccgt cttcaattag atgaacgatt agctaaatat
     2341 gtagaaaaaa ttcaggtcga tccaaagaaa ggaattggat cttctagacg gacctttgta
     2401 agaattaatg attcgctggg tagacctaca aatatttgga aggtcaatta tattcgtgca
     2461 agtgatggat tatttgctgg ggcagaaaca accgatacgc aaaccgctcc taatggggta
     2521 attacgtttg aaaagagcct agatgaaatt tttaaagaga taggcataga caatctcaag
     2581 accgatcgtt taatgtatcg aatctacttg gtaagtcatc aagacgatga taaaattgta
     2641 ccgggtattg atagtacggg ctacttttta acagattcag atgatttcta taatagctta
     2701 gatgtatctg aaaataatcc tgaccagttt aaacatggat cagtaagtgc aaaatatgag
     2761 aaacctaata ctcaaacaaa agatggttca ggatccacag gtgctaatgg agctataatc
     2821 ttagatcata agttaacgaa aaattataat ttctcatatt catcttccgc aaagggcacg
     2881 ccttggtatg ctaattacaa aattgatgaa cgattagttc catatgtagc gggtatacag
     2941 atgcacatgg ttcaggctga caaagtaaca tatgatgttt cgtttgaatc cggaacaaaa
     3001 gtagctgatt tagcaatcga gcgacgggaa ggtcatgaaa attatggcat aggttcaata
     3061 actaataatg atttaactaa acttatcgat tttgctaatg caagtccacg tccagtcgtt
     3121 attcgatatg ttttacaatt gactaagcca ttagatgaaa tcttagaaga tatgaaagca
     3181 acagcacaag ttgaagaaaa taaaccattt ggtgaagatt tcatctttga ttcttggttg
     3241 tcggatacga ataaaaaatt aatccagaac acttatggaa caggttatta ttatttgcaa
     3301 gatattgatg gtgatggaaa ccctgacgat aaagaagaga gcggagacac gaatccatat
     3361 atcgggaaac ctgaattaga agaagtatat gatgttgaca caacagttaa ggggaaagta
     3421 ttcatccacg agttagcggg aacaggtcac aaagcccaac ttgttgataa agaaggtact
     3481 gtattagcag aaaaaactat cgctccaaat gaaaaagatg gggctccaat ttcagatact
     3541 gtagaatttg aatttacggg tgtagattca agtaaattaa tcgcgaaaga tgaattaaaa
     3601 atccaaatcg tttctccagg ttttgataaa ccagaagaag gttcaaccgt tattaaggaa
     3661 tcaccaaaag cggttgataa acaaaccgtg gtagttggat ttaaaccaga tgctaaagaa
     3721 tcaattcgga ataataaaaa cttacctgaa gatgcagagt attcatggaa aacagagcct
     3781 gatacttcta acgttactga tagtacgaaa ggtattgtaa ctgttaagat cggaaatcga
     3841 actttcgacg tggatgtaga gtttgctgta aaagcttctc aagctatgga aaatgatgca
     3901 acatacgtac ctataacaac aactccagaa acgacagttc aaagtggaaa acctacattt
     3961 gataaaccag atgttccttt agctaaagat gccttttcaa tttcagatgt ctataataag
     4021 gacttcggta atgcaagtgt tgatgcaaac actggtattg ttacattcac tccagctaaa
     4081 ggtgtaggag aatcggagcc gattactgga acaattccta ttaaaattgt ttaccaagat
     4141 ggttctgtag gcacgaccga tttagcagta actgtaagta aagatattta tgaaaatcca
     4201 ggagaaaaca ttcctgcagg ctaccacaaa gtaaccttca ccgcagg
//
LOCUS       DACEMK010000142         4219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEMK010000142 DACEMK010000000
VERSION     DACEMK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.148"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            627..3266
                     /locus_tag="IW715_002518"
     CDS             627..3266
                     /locus_tag="IW715_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1023095.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3434..4132)
                     /locus_tag="IW715_002519"
     CDS             complement(3434..4132)
                     /locus_tag="IW715_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1023096.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          806 c          911 g         1224 t
ORIGIN      
        1 ttcttctttc tgttctgtcc aaattattct aagatttctc tttaccaaca tcatttgaca
       61 aagagccaga aaaagccgag ctctcgataa gctcggcttt cttgtacctc ggaaggattc
      121 aaataaacag aaatgattgt aactttccat catacatgca aagcacggtg ttgaatttct
      181 tcatgttaaa caaaaatttt gttttcgtta catcttcaac atttgaaaca tctcttgttc
      241 ctattcaatt ttcagacgtt tacgttattc aattgttacg tatcgcatta cttcttgttt
      301 cggatacttc gtgatccaaa catccatacg cattcactat ttataggttt gtactctaca
      361 cgcatgtctg ggtgaaacct gtacttctaa caactttatt tgcttacact gttctatcga
      421 gaaattcttc tgtctatttg atggtatcct ccttccttac atctatataa tagcatcctt
      481 tggtagcgtt tgcaaatata agcactttaa attttacgct ttcttaacaa ataatcgata
      541 agccctttat ttgaagtaaa taaaaaaagg atgttatagt aaaataagta cctttttttg
      601 tacgaagatt ttaaaggagg aataggatgg aagaatacaa gcagacaata gaagaagtaa
      661 agaaggtaac agaagcagat ccagatactg ggttgtcttc acaagaagtg gatgaacggc
      721 gaaaaaagca gggattgaat aaattcgatg aggcgccgaa agaatcgatg atcaaaaaat
      781 tctttcgaag cttatccgat tttacaacga tcatccttct tgtagcagcg gtgatttcct
      841 tttataccgc atttgctaca gaacatgggg atttatttga aggactgctg atcatcgcta
      901 tcgttgtgat caactcggta ttggcgattg tccaagaagg aaacgcagaa aaagcactgg
      961 aatccttgca agatatgaat aaacaaacgg caactgtcat acgagacggc aaagtgacca
     1021 ctgttgagtc agagcaactg gtagtgggtg atattcttgt tttagagtcc ggtgatgcta
     1081 tttctgccga tgcccgtttg atcgaagcat cacaattacg tgtagaagaa tcggcgttga
     1141 ctggagaaag tgaagcagtt gaaaaagatg cagcatttat tgctgaagaa gatgagtcat
     1201 taggtgacca acttaatatg gtatttaaag gatgtacggt tgctgcaggt cgtgggaaag
     1261 cgattgtcac tgccattggt atggctacag aaatgggtaa gatcgcggat cttttgaacg
     1321 aaaatactat gcaaaaaaca cctttacagg tccgattgaa tcagctaggc aaacgaatca
     1381 gtatgatcgc tttagcggct gcggctttag tatttgtgat tggtgaactg caaggagaac
     1441 cactccttga gatgttcatg acttcgattt ccttggctgt tgcagctgta ccggaaacct
     1501 tgactgttat tgttacgttg acacttgcat acggtgtgca gaagatggcg aggaaacatg
     1561 cgatcatccg acagttgcca gcagttgaaa cgctaggaac cgctaatgtt atctgctcag
     1621 acaaaacagg aacgctgaca caaaatgaaa tgcgcgtccg ccgggtatgg agcaaagaag
     1681 atgaagtaac gaatatcgaa gattcaatga cgaacagcgc gatggaaata ttgaaaatgg
     1741 ctgcactatg tacagatgtg acggtcgagc aagaagatga cgaccttgtt atcaaaggta
     1801 atccaacaga agtagccatc gtaagggctg ttgaagagaa ttatcataca aaagcagaat
     1861 tagaagaaaa atatccacga gtaaacgagc ttccttttga ttctgaacga aaaatgatga
     1921 caacagttca ccaaatgggc gaaaaataca tctcaatcac caaaggagca tttgatgttc
     1981 ttgctccgcg ctttagttca ggcgatgtag aacaagcggc gatcgttaat gatcgctttg
     2041 gtaaacgggc actgagagtg attgctgttg gttatgctac ttatgacgaa gaaccgcaag
     2101 atatttcttc tgaagcactg gaaaaagact tacgtttgat tggtctgatc ggaatgatcg
     2161 atccaccacg tccagaaagt aaaggagcaa tcaagcgagc gaaaaaggct ggcatcaaaa
     2221 cagtcatgat cactggagat catgtggtaa ctgccagtgc catagcgaaa gaattaggta
     2281 tcttgaatga tccgtcagaa gcactctcag gatcagaact gcatcaatta tcagacgaag
     2341 aattagatgc gcgtgtcaaa gctctctctg tctatgctcg ggtaacgcca gaagacaaga
     2401 tccggatcgt caaatcttgg caacgtacgg gtgcagtcgt agcgatgact ggggatggtg
     2461 tgaatgatgc accagcgttg aaagcaagta acgttgggtg tgccatgggg atcacaggga
     2521 cagatgtggc gcaaagtgcc gcagatatga ttttgacgga tgacaacttt gcgacgatcg
     2581 tagatgctgt tagtcaaggg cgttcagttt atcagaatat tcgtaaagca atcaactttt
     2641 tactaagctg taatatttcg gagatcttta ttgttttgat tgcgatgctg ttaggatggg
     2701 gagcaccatt tacagctgtg caactactgt ttgtcaatgt agttgctgat ggacttccag
     2761 gatttgcttt aggaagagag ccagcggaac acggcatcat ggatcagcca cccattccga
     2821 aaaatgaagg gatctttgct cgaggtttat tgcaaaaaat tgccataaat gcaggggtct
     2881 tcaccatcgt aaccttgttt ggttattatt taggtagtta tgtcgatact atcagcccat
     2941 gggtagacgc aagtcagcat gtcggtcaaa ctgttgcatt cttgatctta gcttattcat
     3001 cgattctgca tgtgttcaat gtacgcagca gccaatcgat cttcaaagtc aatttagcaa
     3061 ccaataaagc tttggtagaa atggcgttac tggcattagc tattacaacg gctgttgctt
     3121 tgctgccatt tacgcaagaa ctattcggcc tagtacacat cagtttgaat cactggttct
     3181 tggtagggat tttatctatc gtaccaatcg cggtgaatga actgatcaag ttccatcgat
     3241 taccggaagc agaagaagag gaatgattaa ctgtttctaa gttgattttc tttttactgg
     3301 aactatacaa aaaggaggtg tgacatgaat gttgtcacac ctcctttttg ctaatgggct
     3361 gaatgtttct tttactgcct ctcgataaac ggtgttctcc taccaacccc tcggtcacaa
     3421 gtcacactct cttttaaata tcttttggca attgttcata aaatgctgct tttgttgcgg
     3481 aaatgtatgg gctgagccat agataaccaa taccagcagt tgctaatgct aagatatgcc
     3541 agccgataaa tgaaagatct aaccagaaaa gttgtccttt ataaccatcc atcaattttc
     3601 gacttgcagt gatcgtatcc agtactttag gtgtttgtcc tgtttcttgg acgatatcgt
     3661 agtagataaa atagctttga gaataagaat agcttttaat gattccagga ataactaata
     3721 gcaaacccca taatgttgta aagatcgaga tcagcacagc taagccgatc actccgccga
     3781 taaacaaacc tttgaatcca cgaaatgcat ctttgaatgg ttcgatctga tctttttgtc
     3841 cacgtaaaat atcaagataa gtccaagaaa taccgctcat gaacaaagcg ctaatgatgc
     3901 tggaaataat tccgccaccg cctcctgaac taccgcctcc tgaagttgtc gcatctgaga
     3961 cgctcgctga tccatcatat atggtggtgg caaataagat aataggaatc ataataagaa
     4021 gaatagcagc aataccaatc aatgtaggga ttagatttag taaaacagct tgtccccaac
     4081 gtccttttag actctctttt gcttggcgtt ttaattctgc agaagattgc atgatttttc
     4141 cctccttttc tttaataaca ccaatatacc atgtatcaaa aaatatttct gtcataatgt
     4201 ttttctcttt tttgttatt
//
LOCUS       DACEMK010000143         4200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEMK010000143 DACEMK010000000
VERSION     DACEMK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.149"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..1223)
                     /locus_tag="IW715_002520"
     CDS             complement(3..1223)
                     /locus_tag="IW715_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1023097.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="IW715_002521"
     CDS             complement(1243..1848)
                     /locus_tag="IW715_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1023098.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="IW715_002522"
     CDS             complement(1897..2829)
                     /locus_tag="IW715_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1023099.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3254..4111)
                     /locus_tag="IW715_002523"
     CDS             complement(3254..4111)
                     /locus_tag="IW715_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1023100.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1468 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgcttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga gaattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tcgttaatca atagtatcat tttttttcat atgatttaat tcatcaatta
     3301 attttttgtt tttattaatt ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt
     3361 ctgccttcga cttattttta gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta
     3421 agtaataagt caaatggtta tccgaacaaa tatcgattgc gtaatttagt agtgcatcac
     3481 ttgtttccaa atcaccgtct tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca
     3541 cccgccagat atcttctgtt gtacgaatcg gatacgtata aattttttca aacactcgat
     3601 tgaaataaac ttctgctttt tccaactctt tttctctagc atagaccatt ccaatacctg
     3661 tatatgccaa taaacgaaac aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat
     3721 ggaacaacac atccgtaatt ggtgcattcg taaaaatcat ttcaaatcct tttagatagt
     3781 agtaacgtgc aatcagctca gactcgtcaa ttaccttggg atcaatggct gctaaaatct
     3841 gtgtcaattg ttcatattca ctcgtaatca gtgaaaactc tgctttttct agctgttcat
     3901 caagttgtgt attgatcacg ttcatcgaag aaaaaagttc aactaacgga agattgattc
     3961 gattacataa tttgattaaa atttttaacg aagggacttg accattgttc tcaaatttac
     4021 ttaatgttgc ttgtgtacaa attccttcag acacttcttt ttgagataag ttggcagcct
     4081 ttcttgccgt aataaatttt tcaatgttca ttattttctc tccaatcaga cctcacacga
     4141 aaatcatttc tgtctcgatt ttagcatgtt ttctttggga atgctttaat tggttaagat
//
LOCUS       DACEMK010000144         4037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEMK010000144 DACEMK010000000
VERSION     DACEMK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.150"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            178..774
                     /locus_tag="IW715_002524"
     CDS             178..774
                     /locus_tag="IW715_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1023101.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IW715_002525"
     CDS             898..2697
                     /locus_tag="IW715_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1023102.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="IW715_002526"
     CDS             complement(2975..3187)
                     /locus_tag="IW715_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023103.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1186 a          688 c          712 g         1451 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaat
//
LOCUS       DACEMK010000145         4015 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEMK010000145 DACEMK010000000
VERSION     DACEMK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.151"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(176..1462)
                     /locus_tag="IW715_002527"
     CDS             complement(176..1462)
                     /locus_tag="IW715_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1023104.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="IW715_002528"
     CDS             complement(1455..2723)
                     /locus_tag="IW715_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1023105.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="IW715_002529"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="IW715_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1023106.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="IW715_002530"
     CDS             complement(3495..3941)
                     /locus_tag="IW715_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023107.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1137 a          816 c          707 g         1355 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaat
//
LOCUS       DACEMK010000146         3961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEMK010000146 DACEMK010000000
VERSION     DACEMK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.152"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(609..1166)
                     /locus_tag="IW715_002531"
     CDS             complement(609..1166)
                     /locus_tag="IW715_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023108.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1345..1956)
                     /locus_tag="IW715_002532"
     CDS             complement(1345..1956)
                     /locus_tag="IW715_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023109.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2069..2326)
                     /locus_tag="IW715_002533"
     CDS             complement(2069..2326)
                     /locus_tag="IW715_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023110.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2923..3201)
                     /locus_tag="IW715_002534"
     CDS             complement(2923..3201)
                     /locus_tag="IW715_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023111.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3235..3486)
                     /locus_tag="IW715_002535"
     CDS             complement(3235..3486)
                     /locus_tag="IW715_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023112.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1201 a          810 c          526 g         1424 t
ORIGIN      
        1 tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa tggtgtccaa
       61 aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat tatctccacc
      121 aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat tttcatacaa
      181 aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac tagtatccca
      241 atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct tttatgtatg
      301 ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa caagttgttt
      361 ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca
      421 tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc tcagctaata
      481 gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat ttcaatggtt
      541 ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa tggattagta
      601 gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact aataagacgg
      661 attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc tatttcaggt
      721 gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt aggattaggc
      781 tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac ataatatatt
      841 cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc acttgctaaa
      901 tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat
      961 aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata ttctaatcta
     1021 aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa tattattcta
     1081 cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat gattaatatt
     1141 ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc tcacatatta
     1201 gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt cgctatgaat
     1261 tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat gatttggctt
     1321 gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg gttcagatat
     1381 ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt
     1441 aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc ttttaaaacc
     1501 ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta cattttcccc
     1561 catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc caaaaggtag
     1621 atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca tagaacctgc
     1681 gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt taatctgtcc
     1741 atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg gttcgggtat
     1801 tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca aaaaaactcc
     1861 tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc tcattgatcc
     1921 attgtattcc attttattcc ccctattact attcatatcc gcctatctat atcgatcttc
     1981 caatgcagag ataattgctt ccataccttg attttttact gagttaccta tgcgagatat
     2041 cattctccac taaggtgcat catattgtct atctaacatg attaattctc caataggtgt
     2101 atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct accatgtttg
     2161 ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct ggattaaata
     2221 taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct tttgaatgtc
     2281 cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt taccctgacc
     2341 attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc tcctaacttc
     2401 actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc atttaattcg
     2461 gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt atcaataaac
     2521 tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca taatatcatt
     2581 gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa
     2641 gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa ctatttgtaa
     2701 tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga ttcccaagca
     2761 aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc ttatataaat
     2821 tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg cttatatcac
     2881 aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt tttcaaattg
     2941 tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt taacattatc
     3001 atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta taattaacct
     3061 tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat ctctcctaat
     3121 ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat acatactgcc
     3181 tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc gttcttactt
     3241 ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca acaataagaa
     3301 tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat aagtaggatt
     3361 agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag cacaaacacc
     3421 aaagataaca actccaaaca ttgaattttt atacaatgac catttattta taggcttttc
     3481 tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag ttcataagcc
     3541 ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc ttgttttaag
     3601 cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg ccgaaaagtc
     3661 tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac tatttgtgaa
     3721 acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag tagctcttaa
     3781 ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat cccctctaaa
     3841 aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa tagagcatta
     3901 ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca attatttgta
     3961 t
//
LOCUS       DACEMK010000147         3883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEMK010000147 DACEMK010000000
VERSION     DACEMK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.153"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..3123
                     /locus_tag="IW715_002536"
     CDS             82..3123
                     /locus_tag="IW715_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1023113.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="IW715_002537"
     CDS             3313..>3883
                     /locus_tag="IW715_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ1023114.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DACEMK010000148         3856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEMK010000148 DACEMK010000000
VERSION     DACEMK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.154"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(115..1977)
                     /gene="ltrA"
                     /locus_tag="IW715_002538"
     CDS             complement(115..1977)
                     /gene="ltrA"
                     /locus_tag="IW715_002538"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1023115.1"
                     /translation="MENVSASHSIRHMEYYGMIDSFDNLYKESSDNHVFNNLMEIVTS
                     SNNILLAYRNVKGNSGSVSPGVDNKNIDDLKEIPNADFIKIVQTKFSEYKPQPVKRVE
                     IPKPNGKTRPLGIPTIWDRIVQQCLLQVLEPIMEAKFHDKNYGFRPNRSAHHAFAQAV
                     RLAQVSKLTFVVDIDIEGFFDNVNHSKLIKQLWTLGVQDKWLLGVIRAMLKAPIIHKD
                     GRIEHPKKGTPQGGILSPLLANVVLNELDWWISSQWETHPTRHNYDWYHAEKGYWNKG
                     NKYRALRGSKLKEIYIVRYADDFKIFCRKRSDADNIFIAAKSWLKERLKLDISIEKSK
                     VVNLKKQKSEFLGFTMKLVRKRKTFVIETHMCHKAMKKVSDNLAKQIKVIQHTPTKEE
                     LQKQLVKYNSMVIGIHQYYKKATHISVDCPKINQRIMIIIKNRLDVKKNGHPPSDFVK
                     KNYACSKQLRWINGYPMIPLGFVKHSHTSLPKMGICKYTPEGRVLIHKYLTFPIDIIN
                     QLMNMNNPDESIEFRDNRVSKYSSQRGRCRVTKEFLELADIYCHRIIPKNLGGKDNFQ
                     NLVIIHRNIHQLIHENDKARATLLVMEMRITGTQLEIINQFRKKAKNCELSLNL"
     gene            3203..3817
                     /locus_tag="IW715_002539"
     CDS             3203..3817
                     /locus_tag="IW715_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1023116.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT         1100 a          771 c          701 g         1284 t
ORIGIN      
        1 tagcgatagg taatcctttg atacaatctc cagattttcc cccgctccac accgtacgtg
       61 cgactttcgc cgcatacggc gttccatcaa tgtctactaa caggtatcat acagttatag
      121 attcagcgac aattcacaat ttttagcttt ttttcggaac tggttgatta tctcaagttg
      181 agtgcctgta atcctcattt ccattacaag gagtgtggct ctcgctttat cgttctcatg
      241 aataagttga tgaatgttcc gatgaatgat aactaaattt tggaagttat cttttccacc
      301 aagattttta ggtatgatac ggtgacagta aatatctgct agctccaaga actcttttgt
      361 tactcggcat ctgcctcgtt gtgaagagta cttagagaca cgattatccc taaattcaat
      421 actttcgtca gggttattca tattcataag ttggttgata atatcaattg gaaaggtaag
      481 atacttgtgt attagcacac gtccttcggg cgtgtacttg cagattccca ttttgggtaa
      541 tgacgtatgg ctgtgtttga cgaatcctaa aggaatcata ggatagccat taatccatct
      601 taactgcttt gaacatgcat agtttttctt aacgaaatca ctaggcgggt gtccattttt
      661 cttaacgtcc aatcgatttt taatgataat cattatacgc tgattaattt tcggacagtc
      721 cacactgatg tgggtagcct ttttgtaata ttgatgaatc cctatcacca tcgagttgta
      781 tttaactagc tgcttttgta actcttcttt ggtgggggtg tgttgaatta cctttatttg
      841 ttttgctaga ttatcagata cttttttcat agctttatgg cacatgtgcg tttctatgac
      901 aaaggttttt cttttgcgaa ccaatttcat agtaaaaccg agaaactcgg atttctgttt
      961 tttcagatta accactttag atttttctat gctaatatcg agtttcagcc gttcctttag
     1021 ccacgatttg gcggctataa atatattgtc tgcatcgcta cgttttctgc aaaagatttt
     1081 aaagtcgtcg gcatatctta cgatataaat ctcttttagt ttcgaaccac gcaacgctcg
     1141 atatttattt cctttgttcc agtaaccttt ctcggcatga taccaatcat aattatgcct
     1201 tgtagggtgg gtttcccact gtgatgatat ccaccagtct agttcattta gcacaacgtt
     1261 tgcgaggagt ggggaaagta ttccgccttg cggagttcct ttcttagggt gctcaattcg
     1321 gccatcctta tggatgatag gtgctttaag cattgcccta ataacaccta aaagccactt
     1381 atcttgaact cctaatgtcc agagctgttt gataagcttt gaatggttta cgttgtcaaa
     1441 gaatccctca atatctatat ctactacaaa agtaagttta gacacttgtg ctaatcgtac
     1501 tgcttgagcg aacgcatggt gcgccgatcg attaggtcgg aaaccataat tcttatcatg
     1561 gaattttgct tccatgatag gttctaagac ttgtaataga cattgttgta cgattctgtc
     1621 ccagatagtt gggataccaa gtggacgagt tttcccgttt ggttttggga tttctactct
     1681 tttaactggt tgtggtttgt attctgaaaa tttggtttgc acgattttga taaaatccgc
     1741 attaggtatt tcttttaaat catcaatatt cttgttatca acgcctggag atacgctccc
     1801 agagtttcct ttaacatttc tatacgctaa taagatatta ttcgacgaag ttacaatttc
     1861 cataaggtta ttgaaaacgt gattatcgct actttctttg tatagattgt caaagctgtc
     1921 aatcatgcca tagtattcca tatggcggat tgagtgtgat gctgatacgt tttccatagc
     1981 accgcctccc ttcggatgtc ggtgtttatt ttgtcatact cgattctatg gattgcatca
     2041 taactgttag taaaactaat tgactcgtgg ctatccctcc acagcttgtt atcactgctt
     2101 cataggtact gtgccactac tctcactagt gtgaatgagc ttattattct tcgtctcaac
     2161 cgcttcaaag gaggttagtc ctccacgtca ctagctttca acgttccaat gattttatcc
     2221 atacatttac ttttaggtgc tcactctgaa cctgcgactt tccagtgcct ataacactgc
     2281 acggttattc atatctacga ttcttactaa accgcaagtc gcaccctgtg tcacaatata
     2341 gattttgtgc ggacaagata catttctgca ccccaagcct atagattcgt acattcgcaa
     2401 gtttgtcagt gcaattgcac attctcacca tgagtaattt ttggactccc ggcctatagg
     2461 ttacactatc caccgctgga cagctttcga ttgcagcaat gcaactgcgg taactctcag
     2521 ccgactttac cgagctcttg acaattctta ttctgaccaa gagaattgcc aatcggagta
     2581 tcagagggag cgcttcagga cgttacgcca tcatttcact cctcaaatca tcagttctcc
     2641 acaagaagaa cttgtgtgcc taaccttttc agttaggaac gtttcgcacc gtaaaagctt
     2701 ttcatatcga ttaaaaagta atcatttact ggctcttttg aataatccat cataaataac
     2761 actccattct ttcacattat acaaacatat gttcgatatt gtaaagaaga aatagctaaa
     2821 tacataaaaa aagaagcata ggtatgctat caccctgctt cttcttttga tttgtatgat
     2881 caatttattc tatcacttta ctaattaatt tgaaaatcta ataaaaactc tcactctaaa
     2941 agagtgagag actgcaaatt agagtacgga cttttttgga gttccgttct ttaatattaa
     3001 caaatgaata aaatccttac aacatttgag aataagttat cagtagacac ttacaaaaaa
     3061 ttctcatact atttcctcca tcattagttt ttgtctatat aaggtttacc ctaaatagac
     3121 actggtataa cattgaaaaa tatggcttcc tcgtgtatga ttagggtgta ttttaaatag
     3181 ttgcagaaag taggtaaaag ttttgagagt tgcatacata cgtgtctcat cgatcgatca
     3241 aaatgaacaa cgccagattg aagaaatgaa aaaattcggt gcagagagaa ttttcatcga
     3301 gaaacaatct ggcgcaacga ttactcatcg acctgttttc caagaagcat tagattacgt
     3361 ccgagatcaa gatattttta ttgtggaagc tattgatcga cttggaagaa attatgacga
     3421 gatcatcgct tcagtcaatt acttgaagaa gaaaaatgtg aagctgatca ttactagttt
     3481 accgatcatg gctgaagcaa ttggcaatcc tctcctggat aaattcatta aggatctgat
     3541 catccaaatt ttggccatga tcgctgaaca agaacggact gaatcgaaac gtcgccaggc
     3601 acaaggaatt aagatcgcaa aagcaaacgg cgtctacaaa gggcggccca aattatacag
     3661 tgcagacgca aaagatcccc aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa
     3721 aaatggtgtt gcgattgcta agattgctaa agactacaat gttacgagac aaaccgtgta
     3781 tagaataaaa aaagacagca tggtcaatga caaatagttg atcatgctgc ttttattatc
     3841 ttctcatctt taattt
//
LOCUS       DACEMK010000149         3855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEMK010000149 DACEMK010000000
VERSION     DACEMK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.155"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(872..1912)
                     /locus_tag="IW715_002540"
     CDS             complement(872..1912)
                     /locus_tag="IW715_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1023117.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2261..2587)
                     /locus_tag="IW715_002541"
     CDS             complement(2261..2587)
                     /locus_tag="IW715_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023118.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2574..3362)
                     /locus_tag="IW715_002542"
     CDS             complement(2574..3362)
                     /locus_tag="IW715_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1023119.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1195 a          643 c          526 g         1491 t
ORIGIN      
        1 aatatcttcg agactcaaaa ttcctgtata tgacataaaa taatccaccc acttacttta
       61 aatattttta acaagttttt cttgttattt ttcaaaaata gagaattgat tttcgttcat
      121 gacaaacaaa aaagagggaa cgcatccaaa catgcgttcc ctctaatttc agcgttcgtc
      181 atacggcaaa gagccacacg gacattttta taacattgtg cttaggcata gattcatcgt
      241 aatatcacgt atttttaacc ttaacacagc caaaaaaagt ggtatcactt ttgaatatct
      301 gttatactaa atccgttacg ggtaataccg taacactgaa gggcacgcat taaggcgtca
      361 tcttaatgct ttcctgtagg acactgacgg agattaccag tctcccgagc cttacagggg
      421 cctttctttt ttttgtattt tcaattatca caatttatcg tccaaaagtg cagaacgaaa
      481 atcaattcat cacaagcata tcagacaatt aaatttaaaa caactaaaaa tatacaaaaa
      541 agtgtaattg aatattagca gcactgcctg aaaagaagta atatcaggct tttcagcttg
      601 gggaaataag tgaattacct ctcttcatga attattataa ctcaaaaaag cacatatgca
      661 aacactagtt ccctattttt ttgaatgaaa aataactaaa atatctaacg attatgactt
      721 taaaacctac gtttgtgatt gttttcataa acttttaatc taaaaactcc ggaataatag
      781 ctatttattt gtaacatttg taataaaatt aaaatcataa aaaagttcta atttaaaatt
      841 gatcattttt atagtgatcg ctttttatca actactctag ccaattttcc gtactaactg
      901 ttggtaattc gttaaatgtt tctctttgtt cgtttgcaag ctttgccgca atagcttttt
      961 cgacaaacca attttttaca taaacgaaaa ttagattctg caaattgttt actttttcag
     1021 ttttttgttt ttgataagct ttcagcaaag tcatatataa tcctttgtca gcatcaattc
     1081 cataagattc cagttcttca taaacaacaa ctgtttgctc taattcttca gcttttttct
     1141 ttgcattatg aatagtttgg acggtttttt gagcgtcttc gtaagtacta gaaaatgttt
     1201 ttatcaagtt taatacattt tctggtacaa atgtatttat actgtcctct ttcatgatgt
     1261 cgacgaagtt atctagtaaa acttgatttt gtaactgatc agtttcagtg tctattatgt
     1321 gtctattagt gtctatagaa ttagaataga atagattgtc tgcgattttc gcaattacgg
     1381 ttgagtcctt gatatgactg gattttgccg tatttgcgat tttcgcaatt acggtggtat
     1441 cattgatttc tcgcggtttt gccgtatttg cgattttcgc aattacggtg gtatcagtat
     1501 tgtcttcctc ttccatgatt ttaaagacgt cttgattcgt tacttctggc ttgcctaagt
     1561 acaataaatt aggcatggat tcaatcttac cgtcacgttt atttactctt cctctcttct
     1621 gtttcagaag acctgctttt tctaattcct ttttgatttt agatacttta ccttcgcggc
     1681 attgtaacaa tttcattaat tcttcaactg tgaatatgaa aaatatatta tcatcagaat
     1741 ctacccagtt atttttgact gaataatcaa atcgatcttt aagtagcata taagcaattt
     1801 ttgcatcatt tgatagatcc atgtattttt cgttagtaaa aaatacctta ggcatttgat
     1861 aatatttttc acgataaatt tcttttgatt tgtaataatt aaaatcattc atagccaatc
     1921 ctctttctgt aggatagcgt tttgttcaaa actatgttac aatgaattaa acaaatatat
     1981 ctatccacta tatatttttc tactgctgat aagtcgccaa actttgttaa agcagtagtc
     2041 tttttttatc tttttttagt ttaccttaaa aaataaatta aagcaaaccg ctctatttca
     2101 aggtttaagc tcatagtaac tatatcacga tattacaata atattatatc acgatatcgt
     2161 aatattacaa tatgaagata agcgacttat cagctttgat actactgatt tttataaaaa
     2221 aaatatcacg atattatgat atcgtgatat tgaatgtaaa ttaaatttta ttttgtttct
     2281 ttgctagttt atatccttca atcaagtatt cttcaggtat ggactttaat agcgcatctt
     2341 ctataacgtc tggtttactt ttgtctgatt cagcaatagt cgtccaatat ttgagtgctg
     2401 acattagaat tggatttata cgaatgctat ctgctgttct tacactactt ccttgatctt
     2461 ttatgacttt acttatattt ttcttggtta attcgatttc ttctacttct tttgatttaa
     2521 aattcttttt atttttattt cctagtaatc tacttactgc agcttgactg cgattatctt
     2581 ctgccatcaa aatcaccaac ttcttttaaa tattcttctg taaatgcttt atagtcatct
     2641 tcagctgttg agggtttatt aaaattgatt agatctgatc cagcagcttg cgcttcgtca
     2701 acggttattg tcctacgaat ataagttttg aaaatttttg caccaatgac tctagaaatt
     2761 tcggttgctg cagacgtcat ttctttagaa atgttataac gcttattata cttagtaaat
     2821 agaactcctc gtacttcaag cttgtcgttc gtatattctt ttaccgtttc aatagtttca
     2881 cttaacttta ttagtccttt taatgagaaa tacgcagctt caactggaat gataatttca
     2941 tcagaaacag taaatgcgtt gactgtaagc attcctaaac ttggtggaca atcaattata
     3001 atataatcat agttgtcctt gattggttga agtactcttt taagcctgtg ttcacttcct
     3061 acttgagtaa attccctctc tgcagaagaa agggctatgt cggctggcaa aacatcaatt
     3121 cctttatagt ccagaatagc atctgtaatg tcagcctccc cttttaatac ttcataaatt
     3181 gtaggttggt ttgttccatc tatatcgaaa cctacattat ctgtagcatt tccctgagga
     3241 tcaatgtcca ctaaaagtac atttttaccg taaacaaatt tcaaacatgc tgctaaatta
     3301 acgctagtcg tagttttaga aactccacct ttttgattgg ctactgacaa tattcttgcc
     3361 attaaaatat cccccattca tcttatatta taatataatt atatcataat attgtgatat
     3421 tgcaatatcg tatatgcatt atattgtaat attgtgtgat tattttgttt taaagatata
     3481 tatcggttcg gatataaaga ttattcatat gttattcgaa attaaaataa tcacttttta
     3541 ttactttaaa attttttatt aatgataaga aatatgtttt gcgagcagca actagaataa
     3601 tttaaacatt actaaactgt ctatattgtt actaataatt cccttcttat agtgcatttt
     3661 cttagaaaaa aacaatatta cgatatcatg atattaagat atcgtaatat tatacaattg
     3721 tgattgatat tatatatgtt tttttatttt tttatcatgt ttaatcttga aggaatcata
     3781 ttctttttct aaagacagga gcttttcata aacatcagac atgtttaaac tagaggccag
     3841 gcttaaatca taaac
//
LOCUS       DACEMK010000150         3812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEMK010000150 DACEMK010000000
VERSION     DACEMK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.156"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            94..2193
                     /locus_tag="IW715_002543"
     CDS             94..2193
                     /locus_tag="IW715_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1023120.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2389..3252
                     /locus_tag="IW715_002544"
     CDS             2389..3252
                     /locus_tag="IW715_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1023121.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3357..>3812
                     /locus_tag="IW715_002545"
     CDS             3357..>3812
                     /locus_tag="IW715_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1023122.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1526 a          636 c          772 g          878 t
ORIGIN      
        1 agtaaatcct aaaaataagc tattggcgtg acgtatagaa tatgatagat taaatggtag
       61 cgtaaaccta gtaaaggagc cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg
      121 aaaaaaatgg agcagaatac tatctgtcca gactgcggtc atcaaataac tgcagaagat
      181 cagcaaataa aagaatcaat tgaagaaaca aatacagatg cagctatgca ggataaatca
      241 actgaagatg cagtctttcc tgatacagaa ttaaacgatc caatcgaatg gtcggaattg
      301 aaagatttac ctctagaatc tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa
      361 gaaccacctg ctcacgatca agtaactaat atagagaaaa atgaacagaa gaaagcaact
      421 gttcatgaga cttcggaaga atcaggaaag aatcaagaag aaacgcttga atcaaaacaa
      481 aaaagaagag tagctgaact gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta
      541 tccgcttata tcaaagcaca tcgtgaagat acaaaagaag agcacgcgaa agagctgctc
      601 aaaatgatca gtgaaaaaat tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa
      661 actgaaaaag actctgatag agaaaacgcc acaggtaaag aaacagtaaa aataaaagac
      721 gtaaaaacgg atatcacaga aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa
      781 aaaacgaata aactgccgga agcagagaaa aaagaaatac tcgaaaacac agacaaaaag
      841 tcaacagagc ccgatgaaat aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa
      901 gagggtactg atcttgaagc aaaaatagaa aaagaagaga accagcctaa gctgtttgaa
      961 gcagaagcga aatcaataac aatcgtgcca gatactgaaa aaacacatca agaatcaaac
     1021 ttagaaaaaa cagatccaga taaagcgcaa aagaaaaaat caaaaaaagt accttatctc
     1081 gttttagcag cagtacttct actcggtgga ggaagctggg cttattatga ccatcatcaa
     1141 aaagtagaag ccgaagtagc agcagaagca aaaagacagc aacaaatgga agaacttcat
     1201 gagaaacttg ctgcctttta tacagatgat caccatcaat ttattcgtgc agatatgatt
     1261 catcaagatt tatcaaaact aaaaaatgat ttatccagtc ttaagaatga aaaaggatat
     1321 tcagagttag aaacaactta ccaagatatc caatctaaga tccgagatat ccaaaaagtg
     1381 aatgagtggt tcatagcacc tgccatcgtc gatgatcatc tagcagatga catcaagttg
     1441 aaagcagata aagagatcca agcaatcgaa acggataaca cagattttgg aaaattgatt
     1501 caacaagcac aaacagaagc taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa
     1561 acagctatag tctttaaaga cggaaaagta gtggattcag caacacgcga ccaatacaag
     1621 acggcaaaag aagcagtaga aaaagtgaaa aatacagatc tatctgctca gttgaatgaa
     1681 caattgaaac aagtagataa gatgctcact gataaagaga aaaaagaaga agaggaaaag
     1741 aaaaaagcag aagaagcgaa aaaagcagct gaagaacaag caaaacaagc cgctcaagca
     1801 gcagctgcag ctcaagccgc tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg
     1861 gcatcctcga ccccttcctc ttcaaactca gcgaatcagc caatcaaatc tgccagagca
     1921 agtgatattg ccgatgcatc taatccagca tggacgtggg caccaggtgt ccaaagcaaa
     1981 gtgattgcta catgtatcca acgtggatat atcgttgaag gaggttacag attagaaaaa
     2041 gccagaatcg aaaatgggga aggctattat aatctatatg ccacttcaac aaaatctgca
     2101 ttgatgaaag gcattggaga aagtgctttg ccattttaca tcgtaaccat caactgtaaa
     2161 actggatggt tcggaggaaa tgggagcaat taattttagg caaatgaaag aggctgtgag
     2221 cctgccgttt aacaccgttt attcgaaaaa ggggagtatg acacaactgt tgtcatactc
     2281 cttttttcac ctttccaaac caaggctcag ctattcaagt agataacatt atgttatgat
     2341 agcaaagaaa tgtattaaga aaacctttca ctatagaggg agaaaaaaat gaacgattta
     2401 ttagcaacta tatttacagg tattattatt gatgaaaatg aaaaaaacta ctttgtccaa
     2461 aaaaacggac aaacctttgg cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca
     2521 gttgaaggat tcggttacca aaatcaaaaa catgaaaatc ggatcactac acaaattcct
     2581 aaaagccgta tcggtcatta cgcttttgga atcgttactg ctacacgaaa agatttaggt
     2641 gcgtttgtcg atatcggtct tccagataaa gactttgtcg tatctcttga tgaattgccg
     2701 acaatgacag agctatggcc taaaaaaggg gatcgcttga tgatttccct tagagttgat
     2761 gcaaaagacc gtatttgggg cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa
     2821 tatggaacag aagaaatgaa aaacaaagat gtgataggaa cagcataccg cttgaaatta
     2881 acaggtacgt acgttttaac ggaagatttc tatcttgggt ttatccatcc ttctgaacga
     2941 tataatgaac ctcgtctagg cgagcagata aaaggtcgag tgattggtgt ccgaccagac
     3001 ggtgtactga atctttcatt gaagccaaga gcatatgaag ctatatcaga tgatgcacaa
     3061 atgattttaa ccattttaga acgttctgca gatcagcaaa tcaactttac agataaatcg
     3121 gatccagaag aaatcatgaa agcttttggt atcagcaaag gagcatttaa gcgcgcatta
     3181 ggtaatcttt tgaaacaagg attgattaca caagaaaacg gtgtgacaaa actagtcaaa
     3241 aaatctaatt gagaatccct tgcatgtttt tcttaactag cttataataa ttttaattaa
     3301 catagtgtta tatctttttt tatttgtaat tattataaat tgttggggtt gagtaaatgg
     3361 tcatgaatgc tatttcggcg atgaaaaaaa caaaacagca gctacatgaa gcggggttta
     3421 agctgacacc gcaaagagaa gctactctgc ttgtgctttt ggaaaatgag aaagagcatt
     3481 tatctgcaga agaaatcttc tttttagtga aaaaaaagag tccggaaatt ggacttgcta
     3541 ctgtttatcg aacccttgaa atcttgacag aattgaaaat tgtcgataaa gtaagtttta
     3601 atgatggatt agcccgttat gatttgagaa aagaaggcgc caaacatttc catcatcatt
     3661 tactatgctt gaactgcggc acgatcgagg aagtagaaga ggatctttta cttgaagtgg
     3721 agcaagtagt agaagaacgt tatcattttt tagtaaaaga tcatcggcta acgttccatg
     3781 gaatctgtca ggaatgctat gaaaaagaaa aa
//
LOCUS       DACEMK010000151         3796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEMK010000151 DACEMK010000000
VERSION     DACEMK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.157"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..311)
                     /locus_tag="IW715_002546"
     CDS             complement(<1..311)
                     /locus_tag="IW715_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1023123.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CS"
     gene            1053..1370
                     /locus_tag="IW715_002547"
     CDS             1053..1370
                     /locus_tag="IW715_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1023124.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1461..1826)
                     /locus_tag="IW715_002548"
     CDS             complement(1461..1826)
                     /locus_tag="IW715_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023125.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2034..2516
                     /locus_tag="IW715_002549"
     CDS             2034..2516
                     /locus_tag="IW715_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023126.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2713..3603
                     /gene="pstS"
                     /locus_tag="IW715_002550"
     CDS             2713..3603
                     /gene="pstS"
                     /locus_tag="IW715_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1023127.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1286 a          592 c          693 g         1225 t
ORIGIN      
        1 gaacaactaa taggaaacgc tgcacgacca acaatcacat cgggtgtcca tccttgaaca
       61 acctttcttt gaatatattc tgattgttcc tcgggtaaaa caagcggacg tctaccacag
      121 tttgatttgt ttttcttata ctgttgataa tattctaagg ctgattttcc ttgcttgaag
      181 aacagatata ctttatggat cgtttgtctt gaacgattca agcaatgggc agttttagca
      241 acagattgat tcattttgaa ataagactct attattacaa gttcatccgt tgtaagatgg
      301 gtataggtca tttgtactca ctccttataa ttctttggtc ggaactattt tgagtgtagc
      361 acaaatgact tttttagttg tctagcttaa ttttacaatc ggcgacttat tgcttttgca
      421 aaatttattt acctgttttc tggatcaacg atagtgtaca ttatgctatc cttgtttgga
      481 ttttaggacg tttaaagagg cactttttta cataacttaa tccatagacc ttttaagcat
      541 gctgtgagat aataataaag aagagtttaa agcgcacccc aaaccacttc cccataagtg
      601 tattacgctt taaactcttt tatatttgaa ttcactaaga agcataccat atatttgaat
      661 ttactaagaa gcataccata tttttgaaaa aaagtgagaa aaaaggctta taattaggat
      721 ttagagtaat taattagatg tttattttat aggactgata ctatagatta caaatagtat
      781 cgtatttcgc aatcttaaaa ttatcttctt actgtgaata agataataaa atttgtattt
      841 gtccttttta ggtaacttat tttaatctat atacctttct ggatcaacga aagtatactt
      901 tatatagtca taacgccgat ggtaactatg cactgtaacg ataatgctgc taaaatatct
      961 ttttacgaaa aacatcacaa ggaaaaactt aactatggta ttatctaaac ataatttttt
     1021 cggtatttaa tatttaatta ggaggtaata ttttgctttt ttatagtaat tttatcttaa
     1081 tagtagcgat attattactt ctgaatatat ggatttttga taggagtaga aattcaagta
     1141 taggttttag aactaaacga agtttgtctt ctaaaaaaaa ttgggtatac tcacaaacta
     1201 ttttttatgg aggaattgtt ttaattagtt tactttcttc aacactttat tctttgaata
     1261 ttatagatgt ttccacctca aattcaatat ctattattgg aatcattatt gctgcaatta
     1321 ttacacaact gtttttagtg tttggagaga agaaaagaag taaaaaatag aaaaactagt
     1381 gatagttttg gtgatataag aaaaacatat atgtataaaa agaagaaagt agcttctctt
     1441 tgtacatata tggtttgttt ttatggatgc cgagcttttt tataagataa ggctaccgcc
     1501 cataacgacc caaggatttg tggaactaag taaatacctg caagaagcaa gatcagctgg
     1561 gtcggtttta aaggatgata gaagtaacgc cattcagtaa aattggcgca aaataaatag
     1621 gaagtgacag tagatataat attggcaatg aataaagata taatacgcct gttcctttga
     1681 aaataatatc cagcaaaaag agataaaaat atcaatgtga tgaacccgct tacggaatga
     1741 taacgaaaat caagcagtaa ggcgaaatgc acataaggaa caaaagataa aagcaacgga
     1801 aggtttttcc agcaagtttt tatcatcttt catcaccacc taacaagtgt tattattttg
     1861 ttagttttat tgtaaattga atcctgcaat aatcaattac tttttcatta tttctagatt
     1921 aaatgaataa actctcatgc cattatctct aaaaaaaaag tttttccccc taattttttt
     1981 agatagaaaa ttgtatagta tgatgagaga aaaagtagtt tgggggaacg ttaatgagaa
     2041 gaaaagcagt aatcgatcgg ctttatttat ttttttggtt ttttcctatc gttttgctac
     2101 cttttacatg ggtaaatgct gttgtacaag gtgcggagat attaggcctg atccaaggaa
     2161 gcggatttgt ggtttctagg aattttccag ctttgattgt tttctattta gtgataagtg
     2221 taggtctctt attaccaaaa tctctgatca atcactacaa ttggcagctg ctttactttt
     2281 cttgcttatt agtatttaca tctatgtttt ctttgctgcc tcgcttgatt atcggcccta
     2341 atggtttgag cgagagtgga ttgaaggatt tagcttattc ctccatgtat ttcttgacga
     2401 tcaaaccatg gttctattta gtaattgcct catttattct tgcaacattt ttttacttct
     2461 ggacagaaat caggataaaa agtgaacgat ctgtatctat aaaaaataac tattgatggt
     2521 ggatcatcag taaaaaaaca tatgagctgc gtaaaatgca tgtttctcat atgctttttt
     2581 tattattatg taaagaatgc gaaaaacgtt tacatgattt ttacataact taactatatt
     2641 tttacagcaa ccatacgagg gatttacagt cgactgataa actttagttg taaataagaa
     2701 aggagcaata aaatgaaaaa agtattgttt actctaggta tgatgggaat tcttttagcc
     2761 ggatgcggct caggatcagg aaatacagat ggctctgcca cacaaaataa ttcaagtgac
     2821 tcaaatgaac aagtaaaaat cgttgcggta ggttcgacag cattacagcc gttagtcgat
     2881 gcagcacaag aaagctttgt acaagaaaat ccaaattatc aaatctctgt acaaggagga
     2941 ggaagcggaa ctggtttgag ccaagtcgaa gctggagcag ttactatcgg aaactctgat
     3001 gtatttgcag aagaaagaga cggagttgat gcttcaaaat tagtggatca taaagtagca
     3061 gtggtcggga tggcaccaat cgtcaataaa gatacagatg tcaaagatat caccaaacag
     3121 gaattgattg atatctttac aggaaaaatc actaactgga aggaagttgg cgggaaagat
     3181 caaaaaatca acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa
     3241 tggggactgg atggtgctac ccctgtgcag tcccaagaac aagattcatc aggaacagtt
     3301 cggcagctgg taagtcaaac accaggagca atcagctatt tagctttctc atatctcgat
     3361 gattccacac aagcactaag tatcgacggt gtagaaccaa aagaagagaa tgtagcagat
     3421 aacagctggg gaatctggtc atatgaacat atgtacacga acggaaaacc ttcccctgaa
     3481 gtccaaaaat tcttagacta catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa
     3541 ttgggctatt taccaatcac aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa
     3601 taactagcag ataaacatgc cacggtagtc gttactatta aggaaaaatc aactatgttt
     3661 ccccacatat acatttttcc tttaattgtg aaaaatagtt cctcatattt tgccgttttt
     3721 tgagctaata ccgagggtca gctgattgaa caccgtttat tcgaaaaaaa gagctacgac
     3781 atagaactgt cgtaac
//
LOCUS       DACEMK010000152         3754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEMK010000152 DACEMK010000000
VERSION     DACEMK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.158"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            119..1066
                     /locus_tag="IW715_002551"
     CDS             119..1066
                     /locus_tag="IW715_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ1023128.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1363..1911)
                     /locus_tag="IW715_002552"
     CDS             complement(1363..1911)
                     /locus_tag="IW715_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023129.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1912..2769)
                     /locus_tag="IW715_002553"
     CDS             complement(1912..2769)
                     /locus_tag="IW715_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1023130.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3055..3342)
                     /locus_tag="IW715_002554"
     CDS             complement(3055..3342)
                     /locus_tag="IW715_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1023131.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3332..3661)
                     /locus_tag="IW715_002555"
     CDS             complement(3332..3661)
                     /locus_tag="IW715_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1023132.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         1147 a          679 c          530 g         1398 t
ORIGIN      
        1 tttcatttta aattttaaaa aaatgatgct tatttttaaa aatttaaaaa aagactaaat
       61 tcaatttttt tggtataatg agaaaaaata tctatttgaa aaagttagga gctatactat
      121 gcatctggaa ttctctgact atgaatcact ccttgtaaaa atttgtgaag actattatct
      181 cgaggatatc aatattagtg aaatttctaa aaaatatggg atcaccagat ataaagcttt
      241 aaaatatatt gaagatgcac gtgaaaaaca attagttagt atttcaatta attctccgta
      301 tgctaggaat tatgagctag aagaaatctt caaaaattac tttaaaacga atatctatat
      361 attaaaaagt agtgaagact tatcacaaca cgatttatta ttttcaaaat ttgctgccga
      421 ttacattcaa gaattaatta agaaagccaa tgtggttgca ctatcttggg gagataccgt
      481 ttacaaagtg attgatcagt ttaaaatggg tgctcgtgaa gaattaattt ttactcaatt
      541 cattggcgaa atcggaaaat ataattcctt agctggctcc acacgattag ttcaaaaagc
      601 tgctgaacgt tatgagtctt cttatttgac tctcagtatt cctttatatg tcatcaatga
      661 aactgcacgt gaactaatgg ctctagaacc ttctttagct aataccttag caaccgcttc
      721 gcatgcagat atgttaattt ctggaattgg caccttatcc tctattgatt ctgtcgaacc
      781 ctggaacaag catcgtaaaa tcctatttcc aagcatgacg gatactgttg gatttcttta
      841 cggaagaccc ttcaatatca atggtgaatt catcaaagca tctgaggata agacttttgg
      901 tctttcttta aaagaaattt tcgatattcc aaaacgaatt ggtatttgta aaagtaagtt
      961 caaagcggag agttgtttag gcgccttgaa tggtaatttt tttacagatt tatttttgga
     1021 tgaaaaaact gcttggaaaa tactttcttt gataaaaaaa gagtgagtca tgtaaaatcc
     1081 tccaaaaaat ttagattttt ggtactactt ttttggaggg cagtacaatg attcactctt
     1141 tttattggat atcaaaatcc ctttttttga gaagcttgtc ttgatatttt ttgaactttt
     1201 ccaagccata ctccattact tttcaagatt taatttcttt gtattcactg aataatttcc
     1261 acgttttttc gttaatgtta cttcttatta attttattgt acttctcatg caataggatc
     1321 atatatcctt ctaaatcttt tttatgctat aaaaaggggg ttctatctaa ctttattatt
     1381 cattccccac cctttgcgca gtgagtcttc ggtatcttgt tttatctgac tggtagttct
     1441 tattatttct ccacggtcat ctaattctgg aacaattgga tatttttctc tgaacaagtc
     1501 tagttctttc accacgtcat taaacaaatc ttgcttatct tcttgcgtaa gattctgttt
     1561 aatatactca tctaaagctt ttctaagcaa ttcatctctt gtcatattag tctcatactt
     1621 ttctactaaa actctggaag tcgcatttaa caaagtcgac agttttaaag gatgcttggt
     1681 ggaataaacc ttatcaaact ttctgattgg tgcgccaact ttttttgttc ttcctctact
     1741 ctgtctttca acattggagg aagtttttat ttcttgaact tggtgcactt ctaattgctt
     1801 attttcagat ggtgtaattg cttgctgctt tttctttgct tctgcttctt tttttatttt
     1861 ggcgagtcct ttgttgttcc caaaaatatc aagtcctttg atgtcagcca attactccac
     1921 ctccgcatac caattctcgt gttcttccaa ttctttcaaa atactgataa ataattcgtg
     1981 gcttttgcga tcccattgtt ttaagagacc tttctttgtc ttctccatcg taatacctga
     2041 tccgtcgtat cgttttaagc gcgcattttt gagtacaaca tttttaatca aatgttcccc
     2101 ataaatctct tgggcttgtt tatacatttc ctgatctact gcgtcaccgc tttccaacat
     2161 gaaaggaata acaccagcaa catataaatt tgtatcaaat tctttcacaa aaaagtccat
     2221 atactcaaga tattgcatcg catttctcat cgattttact tgggtctgta agacgacgat
     2281 tgcccagtcc gcaatgtaca tcgcggattt actatataca gaaatagaag gtgggacatc
     2341 taaatagatg acgtcaaact catcaaataa tggttctagc ttttctttca tatatttgaa
     2401 ttgggataat gaatcattat attggtcttt gatccaatca tcatacattt caaaggacat
     2461 atctgctgga agtatataga ggttattgat tactttatag acagcctctt taggtggaag
     2521 attgtttatc aacacgtcag tcacagtatt ttcgtaaaca atcggttcac cttctccgcc
     2581 tccttcttcg ttagtgaagt tcatatgcgt taagttcatc acatcagaag tatttccttg
     2641 catatccaag tcaattatca aggttctttt tccttcatta actgctgatt caaaccctaa
     2701 catttgcgcg cacgtacttt tacctacgcc acctttaaaa ttaccaatta catatttctt
     2761 cgctaccacg ttcctacctc ttttctttta gcaatattca tttgatacta ttataacata
     2821 tttattttta gttactacca aaaaaaagat atatatgtac taccttataa ttaataagta
     2881 aaaggtagta cataaataag gaaaagttga aatatattga tttatagctt tcctcaaagt
     2941 aatgacgtta tttaatttat cgagaagcag agaatactcg cgactttagt catgtagacg
     3001 aatcagattt tcgataaaaa agaagccgtc cttcttaaga acgacaacaa atcactagat
     3061 taattttttc aactgttcat aaaagttttc atgcgttcca cataacaata ctggaataac
     3121 cttgttttct ataatctcgt aagctactct atatgttgtg cccgcatatt tgaacccact
     3181 agaccaaata cctgataaat ccccagtttt ttgttcaccc ttatatggat caatcgcaat
     3241 ttcatcataa attaaattca aaaatttttc tttcaatggc ttctctttta gcttttttaa
     3301 atacttaatg acatgttttg aaggaacgat ttcataagcc aatttctgtt gccaactcct
     3361 ctttagtcat tactttagaa ttagtaagtg tatcttcagc aatatctcta aaggaagaat
     3421 gcattttgtt ttttctattc ttaaattcta caagaatctc atctttatcg tagccttctg
     3481 caattatatc agacaaaata tcttcgctaa aatcataaaa ttctttttgt ggttcaacaa
     3541 attcataaag aataccattc tctaccaact ttgcttcaac ttcgactcca ttaggaacat
     3601 taaaatcttt aggtactgtc aacatgattg aattgccttg ttttcttgtt tttgtaatca
     3661 tttaaatccc tcatttctaa cagtatgtaa ttacttagta aatacattat accacgagat
     3721 aatgtattta caagtaatta ctgtgactcg taat
//
LOCUS       DACEMK010000153         3546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEMK010000153 DACEMK010000000
VERSION     DACEMK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.159"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..1517)
                     /locus_tag="IW715_002556"
     CDS             complement(51..1517)
                     /locus_tag="IW715_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1023133.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IW715_002557"
     CDS             1904..2500
                     /locus_tag="IW715_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1023134.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IW715_002558"
     CDS             complement(2558..3040)
                     /locus_tag="IW715_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023135.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IW715_002559"
     CDS             complement(3146..3343)
                     /locus_tag="IW715_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023136.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1207 a          709 c          578 g         1052 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaat
//
LOCUS       DACEMK010000154         3442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEMK010000154 DACEMK010000000
VERSION     DACEMK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.160"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            38..589
                     /locus_tag="IW715_002560"
     CDS             38..589
                     /locus_tag="IW715_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1023137.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1265..1531
                     /locus_tag="IW715_002561"
     CDS             1265..1531
                     /locus_tag="IW715_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023138.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1589..2101
                     /locus_tag="IW715_002562"
     CDS             1589..2101
                     /locus_tag="IW715_002562"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1023139.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2098..3135
                     /locus_tag="IW715_002563"
     CDS             2098..3135
                     /locus_tag="IW715_002563"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1023140.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3295..3442
                     /locus_tag="IW715_002564"
                     /pseudo
     CDS             3295..3442
                     /locus_tag="IW715_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1263 a          589 c          685 g          905 t
ORIGIN      
        1 gagcgagcaa gacaggaacg agagaacaaa catactcatg aaaagactag aggcttcgac
       61 ctcgaacttt aataataagt tagatgtttt tgaagaaaag actaagcatg caagattaaa
      121 ctttgaaaca actgcaaagc actatattaa acgacttgat gaagataatt tgaaattaga
      181 ttttcaacaa gctattcaag atgaattaag cgatactaaa gacgaaatac gtgaagtgac
      241 gaaacaagcg agagaagaaa caaaagagta taaagaaatc ttagaaagta aaataaaaga
      301 ccataacaaa gtggttgata aatcgaatac agcgctaaaa gtgatgacaa aaggcgttac
      361 aaatatattt tttgtgctaa ttatttttgt acttgtaatg cttgtgacag gtccgatagg
      421 tcatttcttt ggtattgaac atttatatag ctttattaat ggttttattg atgaccacga
      481 aagtgcatgg cgatatttga tgttgatact ttatgcagta ccctatgtat tcttcgctat
      541 cattctgtgg ctcatagcat tgttttttag agtatttaat gacctttagg tagatttata
      601 tataaaaagg gctaaatacg caaaataccc ccttaaaatg caaaataaat gcaaataagg
      661 gggtaactcc ttatgaaaaa acaactaata gtattataac acgtttttat gccgagaaaa
      721 cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt tggacttgaa
      781 ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt aggttccgac
      841 caataaggaa attggaataa agcaacgaaa ggagttgagt aaaatgagtg agaaaacgaa
      901 gaaaatcatt cgagtatcat tagctttttc acaagtagta gtgcaatcat tgatagttta
      961 ttatacttac cagcaatata aattatctaa agagaattaa gttttgatta tgtaataagt
     1021 tttggtacta aaaaagactt gatctaatta gaccaagtct tttgttagtg ttatattaat
     1081 aacaaaataa aaataagaag tcgctcactc cccgaccaaa gtttgtgagc aaaataaaca
     1141 gtcgatgttt gtttcttaat tgtatatctt gatattaaac gatttaagat tattcttcaa
     1201 gatatatatt cgcaaatgta attggaaaag cgacttctta aacaaaatta aggagtcgct
     1261 ttttatggca aaaataacga aaaatgagca agaaaatttg gtggaaaatt acacaaaaaa
     1321 accggcgtac tctaatagcc ggttagacgc acatttcgtg tgcacttcta atccgaaatt
     1381 gagctttgac gctatgacga tagtcggaaa ccttaataaa aacagcgcta aaaagctatc
     1441 cgattttatg agtttagatc cacaaattag actttgggat atacttcaaa caaaatttaa
     1501 agctaaagca ctacaagacc ccctatgtta ggctagttgt cgtataggat aataagttat
     1561 aataaagact atacggagga tgattaccat gacaaataaa aacaatgaac gttactctac
     1621 cgaactgaaa gcgacagcca tcaagaaaat gatgccaccc gaaaataggg ggattcgcca
     1681 attaagtcaa actttgggca ttccagaagc gactctttgt tcttggcgta aaaaagctag
     1741 aattcaaggg aatactacac ccggaacgaa aaaaaatgtc gaaaaatggt caagtgaaga
     1801 taaattttta attgtgatgg aatcattctc aatgaaccaa gtagaacttg cggaatattg
     1861 ccgaaagaag ggcttattta aagaacaaat tgaggcttgg aagaaaacgt gcttatctgc
     1921 gaatgaacag gaagaaaatc gaacgagaga acttgcaact gaactaaaag aagagaaaaa
     1981 acaagcgcgt caattagaga aagaattacg tcataaggaa aaagcattag cggaagccgc
     2041 tgctctctta ttactaagaa aaaaagcaca cgcgatttgg ggggaccaag aggaagaatg
     2101 atttcatctt cagatcgcaa acaagctgtt gaacttattc aagaagccaa tcgcaatggg
     2161 gcccgtctca cctatgcctg taatgagctg aatattagcg ttcgaaccta cgaacgatgg
     2221 acgcgtgaag gtacgatagc gcatgatcaa cgccctttgg cgaaacgtcc tgtacccaaa
     2281 aataaactga cggatcaaga acgcgaaaaa ataatagaaa ccgtttcaaa aaaagaattt
     2341 atgaatttgc cacctagcca aattgtacca aaacttgcgg attgctcgat ttatattgca
     2401 tcagagtcta gcttctatcg tgtattgcga gaaaaaaata tgcaacatca ccgtggccga
     2461 agtcaagttt cacaaaaacg cataccgcca agtcaccttg cgacgaagcc aaatgaagtt
     2521 tggacttggg atattacttg gctaaaaggt ccaataaaag gactctttta ccggttatat
     2581 ctcatcattg atttatttag ccgtaaaatg gtaggctggg agatttggga atcagaagaa
     2641 gctcgtcatg ccgaaaaact tattaaacag acggtgctta aggaacgtat ccaaggacag
     2701 ccgttggttt tacactccga taatggcagc cctatgaaag ccgcgacttt tcaagtctta
     2761 ctcgaaaaat taggaattca aagttcttat tcaagaccac gagtgagtaa tgacaatcct
     2821 tattcggaag cggctttccg aacgttaaaa tatcgaccag aatatcccaa taacggcttt
     2881 aaaacaatag atgatgctcg aaaatggacg aaagagtttg tcgattggta taacgattct
     2941 catttgcata gtgggattca atttgtgtct cctgcagcgt gtcatgaagg acgacatctc
     3001 gctattttag agaaacgaaa gcaggtttac gaggacgcta aaaagcgcca tcctgaacgc
     3061 tggtcaaaag gtgttcgaaa ttggactcca catcaatcgg tggcgttgaa tcctttaaaa
     3121 gaaacagaaa gttaacagga gcataatctt tcaagattat caaataaccc tcgtcaccca
     3181 ataggggaaa aagttattct tcccctattg gataacgagg ccaaccgacc atagggaggt
     3241 tctgcttttt gataggtgga aaataaaaat acgacaacta tattgacaaa caccgaccat
     3301 cgtccagtaa agagacgcaa taaattctat cgaagtttac gcactgcctc tacaacgatt
     3361 aaaggcatgg aagccatccg aggattatat aagaaaaccc gaaaagaagg cactctcttc
     3421 gggttttcgg tctgtactga aa
//
LOCUS       DACEMK010000155         3399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEMK010000155 DACEMK010000000
VERSION     DACEMK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.161"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..277
                     /locus_tag="IW715_002565"
     CDS             <1..277
                     /locus_tag="IW715_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ1023141.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IW715_002566"
     CDS             420..641
                     /locus_tag="IW715_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1023142.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IW715_002567"
     CDS             657..1499
                     /locus_tag="IW715_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023143.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IW715_002568"
     CDS             1562..3364
                     /locus_tag="IW715_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ1023144.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1229 a          469 c          703 g          998 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DACEMK010000156         3382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEMK010000156 DACEMK010000000
VERSION     DACEMK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.162"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            210..476
                     /locus_tag="IW715_002569"
     CDS             210..476
                     /locus_tag="IW715_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1023145.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            503..802
                     /locus_tag="IW715_002570"
     CDS             503..802
                     /locus_tag="IW715_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ1023146.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="IW715_002571"
     CDS             976..1527
                     /locus_tag="IW715_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1023147.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1673..1966
                     /locus_tag="IW715_002572"
     CDS             1673..1966
                     /locus_tag="IW715_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023148.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1959..2255
                     /locus_tag="IW715_002573"
     CDS             1959..2255
                     /locus_tag="IW715_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1023149.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2330..3328
                     /locus_tag="IW715_002574"
     CDS             2330..3328
                     /locus_tag="IW715_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1023150.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1275 a          459 c          666 g          982 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
     1621 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
     1681 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
     1741 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
     1801 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
     1861 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
     1921 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
     1981 gtggtttaat ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag
     2041 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
     2101 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
     2161 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
     2221 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     2281 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     2341 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     2401 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     2461 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     2521 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     2581 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     2641 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     2701 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2761 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2821 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2881 tcaaggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca
     2941 aggtttcagt gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac
     3001 tcaacaagca acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga
     3061 agctgctaaa gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa
     3121 aaatgtatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3181 tgaaaatgga agctactatt caattaatat tgtaacggat gatgatggaa acacgaccat
     3241 ggctggtgga aaacaatttc gtgtttatac agatggaaga atttctcaac gtattggaat
     3301 gactaacaat tttcaaatga ttaaataatt gaaaatatta tttatcaaag aaaaactgat
     3361 tttgagaaaa attatttaat tt
//
LOCUS       DACEMK010000157         3376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEMK010000157 DACEMK010000000
VERSION     DACEMK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.163"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            254..580
                     /locus_tag="IW715_002575"
     CDS             254..580
                     /locus_tag="IW715_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023151.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IW715_002576"
     CDS             complement(697..1041)
                     /locus_tag="IW715_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023152.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IW715_002577"
     CDS             complement(1117..2364)
                     /locus_tag="IW715_002577"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1023153.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IW715_002578"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IW715_002578"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1023154.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          666 c          671 g         1052 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agtagt
//
LOCUS       DACEMK010000158         3373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEMK010000158 DACEMK010000000
VERSION     DACEMK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.164"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW715_002579"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW715_002579"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1023155.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW715_002580"
     CDS             complement(2260..2511)
                     /locus_tag="IW715_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1023156.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IW715_002581"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IW715_002581"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1023157.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          739 c          626 g         1088 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttt
//
LOCUS       DACEMK010000159         3365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEMK010000159 DACEMK010000000
VERSION     DACEMK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.165"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(128..952)
                     /locus_tag="IW715_002582"
     CDS             complement(128..952)
                     /locus_tag="IW715_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023158.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(1590..1847)
                     /locus_tag="IW715_002583"
     CDS             complement(1590..1847)
                     /locus_tag="IW715_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1023159.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1840..2109)
                     /locus_tag="IW715_002584"
     CDS             complement(1840..2109)
                     /locus_tag="IW715_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1023160.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(2240..2419)
                     /locus_tag="IW715_002585"
     CDS             complement(2240..2419)
                     /locus_tag="IW715_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023161.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(2800..>3365)
                     /locus_tag="IW715_002586"
     CDS             complement(2800..>3365)
                     /locus_tag="IW715_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023162.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         1026 a          628 c          435 g         1276 t
ORIGIN      
        1 ttcctacata taattactat ctataaaatt aagtcttact cagaacagat aaagcataaa
       61 attccaacaa accatttttt acttaactaa ataaagagtc tgttaatata atcttcactt
      121 atttacgtta atcccctact gtatttgttg gttccttatc tatattttct acaaaatcct
      181 tattatcttg tgatttagct tcgtataatt ctttgtatgt cgacacattg gttatactaa
      241 ttcctatagc tagttcttga gactctactt ttctctctag ctcctttgac aaagtgattt
      301 gagatttatt tggttccgtt ggagtcaatt ctatagattc ttcataaaaa tctctataca
      361 taaagctatc attaatttta taaaaaaaat taaatgtttt tagcactgcc atataacatt
      421 caaaacattc ttctatagtt ggtttacttc cttgaggaga atgaaccaat ttattacgtt
      481 tttctatgaa atttttaatt tcctttctta tttctttcgg aaactcaata ttaaagtacc
      541 ctttaaacat gctccctaat aatttgtcta cagggggaga ttgtaaattc tcaaatatta
      601 gagttaaatc aggtttctct tgttgataaa attcctttac ccctatttca caggctgtaa
      661 ttatcgaaat aaacctatct ctagtatttg atatattttt acttctttta aggagtataa
      721 gagaaaaggg gatactttcg tgtccatgca tgtaattaac taattgttga gcttcagaag
      781 ttatatctct aattatatag ttaacggaat taggaagtgt ctttaactct aatgaaattt
      841 ctatccaatt actatcttct aatctgtata tgctcgaagt ataatttgat tcgtcagcaa
      901 gcatcgctac atcccaaaca ctgatcataa aatcttttaa acgttcaatc atttcagcaa
      961 tattgctaaa atcagctaat ttttctttag aataaaaaaa aacatttata tcagaatcaa
     1021 taaattctat ttttttatta ctatatcgtc atcattatct tgaccattta tgtttattgt
     1081 tccctgagtg gggagtactt ctttcaaatt caagttaaat ttatattcca ttacttctcc
     1141 tccaatatat aaaaattact taactgtatc caaatttaat atgtaaataa atcattaatt
     1201 tttagctaaa ttctatgaga agtttatttt tgttgttttt gctaattaac taaaactagt
     1261 acaaaaaatt taaaatagat tattaaacct attgcttatt ctattcagtc atatatataa
     1321 gcgtcatcct ttagtaacta acatcccaca ctacaatagt attataacat atctattcaa
     1381 cttcaaattg ttgaaattta agccatttat atttactttt cctcaacttt agaaatgatt
     1441 ttactaacct tatatccgta atacgcgtaa caaacacatt cattaataac aatcaacact
     1501 aagctatatt agctttttaa atgactaggg taaataaaat aaggatcaaa aacttttaag
     1561 tttctgaccc tttccttact tccgattggt taatagtgat cttttgcaga ataaataaat
     1621 atcgtttcat tttcaactct atatatcagt ctatgttcat ctgtaattct tctggaccat
     1681 tttccagata aatcatgctt taatggctca ggttttccta atccagcaaa gggggaacga
     1741 tcgatatctt ttattaactt gttaatcttt tttatattgc ttttgtttcc ttgctcatgc
     1801 caataaagat aatcatccca agcatcatca gaccaagcct taatcatcag attcaacctc
     1861 gattaagtca tgtgttttaa atgcaccttt ggagaattgt tcatctcctc gacgaatttt
     1921 ttccatgacg taattattag aaagtgttct caacgtttct tgcatagaat cataatctct
     1981 ttttgataat acaacaactg tatcttctac atctttactt gttacaataa gtgtttcagc
     2041 atcctcatta acttgtttca tataactacg taaattttgg cggaaatttg aataagctac
     2101 tgcttccatt cctttcaccc cttatttatt tctataaaac aattgtacat tatattgtac
     2161 aattaagcaa atgattaata ttctaacttc ttataattaa aagtgctatc acttttagtt
     2221 tttaggataa aaagtgacgt cactttttga gtgctggtac ctcaaatcct gctttttctt
     2281 gggcaatcaa taaatctaac gcaatacaca taatcctgga ttcacttttg ccgtacttct
     2341 gctttagttg ctgcaattct ttttgtctct tttcatccaa cgtaacgggt aaccgtttat
     2401 ttcctattgc aattggcatt agtactctct cctttcatcg aaataaccct atgaaaaata
     2461 tggctgcaat catcttgaat agtagactca caagggctaa tacaaagcgc caaaagatca
     2521 aacaaaatac cagtattgcc aaaaatacta cgaagtttaa atgcacagct gcagcatggc
     2581 gcataagtat ttcgccaaca aataggacta acaatagagg aatgatttta ttcatatgga
     2641 tctccttctt tctcttaggt tatcgggtgt tgcccgctta tttcgcacca cctaagcggc
     2701 gggggcggtt ggagcgattg gtgcgaaata agaaccattc ccttagttac taggtttttt
     2761 gtccttattt cacacattct cacttttcgg tataaatgat tattgccttt ttttcctttc
     2821 aaatcgttga tatggcattc gtttacggtc aagttcattt tggatttctt ctgtatcgtc
     2881 gtaagtatag gtgtctaaaa aatcagttaa agtcgtgtac cagtcgtcgc ctgggttatc
     2941 tacttcttct acatattccc tcaaaaatgc ttcgggtaca gcgagttcat aatccattcc
     3001 cattaacacg atagattccg ttccacacgt caaaaatcta gtaaatacaa ggtaataatc
     3061 gtctatcgtc gcatctttgc tgtttagctc ttccatttct tcttccatta attccatgct
     3121 ctcaaaaatt gaataatatt ctgattctga gtaatagtgt ttcctgcaaa tcgtgtagct
     3181 gacaaagttt tcgaccagct cactcctttc taggtaaaca ttcatgatgt atttttctgt
     3241 ttctagtcgt agaagctccg ttcctttttt taccgtttca ggacgacgtt tatacacgtc
     3301 taaacgatta tggctgactg tgaccttcgt ttgactcgtt tcactttcca aatacaattg
     3361 ttctt
//
LOCUS       DACEMK010000160         3355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEMK010000160 DACEMK010000000
VERSION     DACEMK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.166"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(121..600)
                     /locus_tag="IW715_002587"
     CDS             complement(121..600)
                     /locus_tag="IW715_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAQ1023163.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(617..1078)
                     /locus_tag="IW715_002588"
     CDS             complement(617..1078)
                     /locus_tag="IW715_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1023164.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(1092..2342)
                     /locus_tag="IW715_002589"
     CDS             complement(1092..2342)
                     /locus_tag="IW715_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1023165.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(2720..>3355)
                     /locus_tag="IW715_002590"
     CDS             complement(2720..>3355)
                     /locus_tag="IW715_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1023166.1"
                     /translation="AFIKRTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQER
                     IGQNGKPFKMYKFRSMYMDAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTK
                     KTGIGQFIRSTSLDEFPQFFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGIT
                     GMWQVSGRSDITDFEEVAKLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
BASE COUNT         1033 a          698 c          455 g         1169 t
ORIGIN      
        1 atattctttt tttcccaatt aaaattgagc atttgtgtaa ttatacttgt aattccacct
       61 tttaccgagc tgtcattacc aatcataagt actctttgca taacgcttta ctccttatat
      121 ttatattttt tctattaata cctctatcga attaataatc gtttccgtgt tagatacata
      181 cttgttaaac atccgaactc ttgtttcttt aacggcatct tctaaatttt ctaattcata
      241 acaaggatag ataatattca tctcatcaaa ttgtctaatt aactgcagtt ggtgatcatc
      301 gacatgctca ccatacttag ccaatcgagg aacagcaatg acccttttac ctttttttac
      361 ggccccaatt atagcacctg tacctccatg agtaatcacc atttctgctt tttgcatagt
      421 ttcagtaaac tcatccctat ttagaaatgc tttataggaa taatttagtg gtttataatc
      481 actataacca ctctgtgcaa atgtactttc gattattact cctttttcaa ttaaatcatc
      541 aatagctttt aacaatctat caaattgaaa tttttgtgaa cctaatgtta taaatatcat
      601 ttttttctcc tattatttaa tatatcccac ccaaataaat agcattagga taaaattgta
      661 gcatagtttc ccactgaaca taaaattgat ctgcaaaatg atacatcaat tttcctgtct
      721 gagtagggct ggttactttt gcaaacgatt caatataaat cagttttttt cccatcattt
      781 ttgttattaa gcagataggg atcatagcta aaacacctgt acaaatcacg agatccggtt
      841 tttcctttat atatataata attgatttga ataaattaat aagtatagta cacaaaaaaa
      901 acttttcttt tctatttact tgtctcatgt agtacgtttt ttcgcctttt gactcagctg
      961 aatattctgt ctgttctgtt ataacaaaac tctcatactt ctccatcagt ggttttaaca
     1021 tcattaactg ttcataatgc cccccagagg aagccgcaaa acatatcttt ttgttcatat
     1081 taacctctct atcagtcacg ctgaaaaata tctctagtgt aaactttttc tttaatatca
     1141 tccaagcagg tatcatatcg attggcaata atagaatcac acatcgcctt aaactgctct
     1201 aaatcgttga taaccttact tccgaaaaaa gtctctccat ctttcagtgc aggctcataa
     1261 atcaccacag tcgcaccttt agcttttacg cgcttcataa ctccctggat tgaactctga
     1321 cgaaaattat cactgttgct cttcatggtc agccggtata taccgattaa aactggattt
     1381 tccctgttga catcatactg attatattca gcgtagttgt aatagcccgc catctgaaga
     1441 acacgatctg cgatgaaatc cttacgggta cgattagatt caacgatagc ctcaatcaaa
     1501 tttccaggaa catctgcata gtttgccaac aactgcttgg tatcttttgg caagcaataa
     1561 cctccatatc caaaggatgg attgttgtag tgagttccaa tacgaggatc gagacaaaca
     1621 ccattgatta tctgctgagt attcagcccc ttcatttcag cataagtgtc taattcattg
     1681 aaatagctga ctcgcagtgc taaataggta tttgcaaaca gctttactgc ttcggcttca
     1741 gtgaatccca taaaaagagt atccacgttc tccttaattg cacttttctg cagaagtgtt
     1801 gcaaaaacat gagcagcatt taacaagcgc tcatcagtca tatcagtacc aacaataata
     1861 cgagatggat acagattatc atataaagcc ttgctctcgc gcagaaactc tggactaaaa
     1921 ataatgttct tactccccgt tgtctcacgg actgattcag tgtagccaac aggaattgta
     1981 gacttgatga ccatgatggt atcaggattc acgtccatca caacctcaat aactgactca
     2041 acagcagaag tatcgaaatg ctgagtacga ctgtcatagt tagtcggtgt agcaataaca
     2101 acgaaatctg cggatttata agcgctcctt gcatccaaag tagcagttaa gtcaagttct
     2161 ttttctgtaa gatacttttc aatatattca tcctgaattg gagacttccg tttattaatc
     2221 agttcaatct tttcagggat aatgtctacc gcggttacct ggtgatgctg cgccaatagt
     2281 gtggcgatac tcaaaccaac ataaccggtg ccggctactg cgatgttgta ctttttcttc
     2341 acaatatctc ctctttcctc tcttaagtat ggagaatgtt actcctacgg ctattcatca
     2401 tgtcaaaata acacagctta gaatgttgat aaaataattt tccagaatgt gataaaatct
     2461 ctttcattta taaaccacac atctaatttt atctattctc cacaagacat gtagagtatt
     2521 ctttaataac aagatacgac cttactaaat acttcaaaaa attatcatat ttaattcata
     2581 aatataaatc ttcattttaa atgtatgccc aaaaatttat ctgtaatgcg tcatcaaaat
     2641 ctagattcca aatctttatc aatttttatc cactcattct accactttag taatattttc
     2701 tatttataac actttcgtcc tacattgatc cccccctctg aagtacagtt tttactgtct
     2761 taaataaaat tttaaaatct aaccccatac tccaattttt tatatattcc gtgtcaagtt
     2821 ttgctacttc ttcaaaatca gtaatatcac ttctaccact tacctgccac attccagtaa
     2881 ttcctggctt gatagcaatt cttgcacgat gatgataatc atattcttca tactctgatt
     2941 taaggcatgg tcgaaatcct ataaggctca tctttcctgt tagaacgttg aaaaattgag
     3001 ggaattcatc taaagacgtg gatctaataa attgaccgat tcctgtcttt tttgttccat
     3061 ctggaagtat tttatttccg ataattcttg gatcaaactc catcttaaac ataagattat
     3121 tgctcatctt attttctttc attaattcta cttttctttc ttctgcatcc atatacatac
     3181 ttctaaactt atacatcttg aacggtttac cattctgacc tatacgttct tgtgcaaaga
     3241 aaattgggcc tggtgaatta atataaataa gtggagcaat aaagataaaa ataattcccg
     3301 ttgcaataca tccaacaata cctgcaagaa tatcaattgt tcgttttata aatgc
//
LOCUS       DACEMK010000161         3347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEMK010000161 DACEMK010000000
VERSION     DACEMK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.167"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..238
                     /locus_tag="IW715_002591"
     CDS             <1..238
                     /locus_tag="IW715_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023167.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="IW715_002592"
     CDS             252..440
                     /locus_tag="IW715_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023168.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="IW715_002593"
     CDS             452..1063
                     /locus_tag="IW715_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ1023169.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="IW715_002594"
     CDS             1048..2439
                     /locus_tag="IW715_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1023170.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="IW715_002595"
     CDS             2655..2957
                     /locus_tag="IW715_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023171.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..3297
                     /locus_tag="IW715_002596"
     CDS             2950..3297
                     /locus_tag="IW715_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023172.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKRLFVKSCW"
BASE COUNT         1306 a          472 c          654 g          915 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa aggctcttcg tcaaatcgtg ttggtgaaga
     3301 caaatttgaa taaaacttag tccgatatgc ggcgcgttct gtgcgcc
//
LOCUS       DACEMK010000162         3299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEMK010000162 DACEMK010000000
VERSION     DACEMK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.168"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            139..1107
                     /locus_tag="IW715_002597"
     CDS             139..1107
                     /locus_tag="IW715_002597"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1023173.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1424..1948)
                     /locus_tag="IW715_002598"
     CDS             complement(1424..1948)
                     /locus_tag="IW715_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1023174.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2235..>3299
                     /gene="scm"
                     /locus_tag="IW715_002599"
     CDS             2235..>3299
                     /gene="scm"
                     /locus_tag="IW715_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1023175.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1053 a          667 c          611 g          968 t
ORIGIN      
        1 aaaaaatgaa cgcccaccct cttctttttt ctctttcctt ctttcgtttt tgctttatgt
       61 atcaaccgtt gtttcttcga aaactatttt gttctctcct tcttcctgat agaatggatg
      121 ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca
      181 ccagattttc tttccggaga aaagttggca cagcaactgg aagtttcccg gacaagtatt
      241 tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg
      301 tatcgttacc tgccctctga cgtactagat gcaaaagaaa tccagcaaga gctacggtcg
      361 tttattcctg atttgaaagt acaaacgatg ttgacttctg aatctacgat gaaagatgcc
      421 aaactggcag cagctcaagg agattctgtt tcgaagttat ttgttgctga tacacaagaa
      481 caagctcgtg gacgttttgg acggcctttt ttcgctgaac ctggacgagg aatctacatg
      541 agtctcttgc tccatccaga caaacggttt gatgaattgc ctcaatatac gatcatcgca
      601 gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc gatcaaatgg
      661 gtcaatgata tctacatgga tggaaaaaaa gtctgtggga ttttatctga agcagtcagt
      721 gatatggaaa gcggcaggat cagtcatatc atcattggga tcgggcttaa tttttctgtt
      781 cctcaaaatc attttccagc tgatctccaa gaaaaagcca tctctgtctt tcccgacgga
      841 caagctagtg tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt gttcacttat
      901 ctttctcacc cagaaaaagc gttggacttc tataaagaaa aatcattcgt attgggaaaa
      961 accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat
     1021 catggcgaac tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc
     1081 agtctcacat ctatccgtac gcaataatta tcagatttca caaaaggaca cctgatctca
     1141 tacagtgtcc ttttttctga attctcttca aaagactctc cttattctat cagctgacag
     1201 tttctctatc ctctagaaaa aggtattcta aaaatatcta taaagcatgg tacactttct
     1261 ttaaatgaat caatgcaaaa aacaagagag tcatcagttt acacgatcaa aagagatagc
     1321 aggccgcttc ctttttcaat cttatagata gaccatgggt aacgataaaa acaaaaaaga
     1381 acttgcgact tattttgccg taagttcttt tttagctagt gccttaatct cttaatccca
     1441 acaaacgtct cgcatagatc acctgaccag agtgcatagc agcatcatca ataatagaaa
     1501 cgagacgcat tcctcgtgtg actggtggtg tccagttctc gtcaattact tcatctagct
     1561 cggaaggttc cagccttctt aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc
     1621 ctagcagatc ttctatttga ttagcttgga tctgctttgc ttgttccaga ctatgcttcc
     1681 aatcttgttc gttatctgct atcgtcatcg gaaaccggct ttcccagtct tgactatgcc
     1741 aaattggaga agttcctgct aaatcagcaa tctggaaatc taattctcga gcggtatgcc
     1801 aagccagcca agtcattgat ttgatctgcg gggctctatc ttccactgga aaacgattgg
     1861 cctcctcaat agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac
     1921 tctcgcatag catatctaaa taatccattt attttcctcc tttttattta tcctacttac
     1981 tttttcctaa ttctaggata cctacttata tgttactaca cacccttatt ttttacactt
     2041 taacagtcac catcatcaat agtttggaga ttgaaagaaa aagatagtgt cgaactatca
     2101 attgaacaag aagcaacaat atgaacgttt cattaatatt ctttaaataa acaatgattt
     2161 ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa taggaaaatg tttgaaagga
     2221 aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt cctagttgct ttgttgttca
     2281 atatcttttc gtatggtgtg gccgtttcag cggaaacata cattcctaaa gaccctggga
     2341 atatttataa tgatctaact ggtaactatg gcttgttagg aattgctagt caattccacg
     2401 tatttgctaa aaataagaca actgtgaatg cacatacaga cggaaatata gcaacacatg
     2461 aacttgatgc aaaaaataat tttggtacag agcttcatga aggtttattg aaccaagaaa
     2521 taaattatat acaaaaaact gattctttcg ctaattcttc aggcatacct acaggtagca
     2581 caagaatgaa taaatttgtc gttggcagta acgtggatgt agcacttcaa gatggtcatc
     2641 ctacagtcaa tggatccagg cttgatcact taactccaga tgatttttat aaagatcgtg
     2701 ctgggcatga atatattgat ttcgagcagg aatttgctaa attgaatgta gcagcaaatg
     2761 atcttgcaac gatcactcca gctaaaacat atactgctgc agattttcca gacatgaaca
     2821 atcggacaat cgatttaact gatttatcag ataccgggtt tttattggta aatatcgatg
     2881 cagaagtgtt aacgatgaat acgccacttc aaatcattaa tccaaatgat caagtagttg
     2941 tatttaatgt catcaacagt tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg
     3001 atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa tatctcttgg aattttggaa
     3061 atgagatgac tgatttgaca atcagtgctc cttttcaagg aacaatcctt gcgcctaatg
     3121 ctactatccg cattactcaa aaccaagacg gaacgattat aggaacaaat gtgattttag
     3181 atgcagctac caatcgttgg gatcctaacg aaatatttat cagcgacaat ggtacagata
     3241 cgacagacac aagcgacagt acagatacat ctaccacaag tgatagctca gattcatca
//
LOCUS       DACEMK010000163         3246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEMK010000163 DACEMK010000000
VERSION     DACEMK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.170"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            198..1152
                     /locus_tag="IW715_002600"
                     /pseudo
     CDS             198..1152
                     /locus_tag="IW715_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1169..2193
                     /locus_tag="IW715_002601"
                     /pseudo
     CDS             1169..2193
                     /locus_tag="IW715_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2199..2678
                     /locus_tag="IW715_002602"
     CDS             2199..2678
                     /locus_tag="IW715_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1023176.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2694..>3246
                     /locus_tag="IW715_002603"
     CDS             2694..>3246
                     /locus_tag="IW715_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1023177.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          983 a          542 c          755 g          966 t
ORIGIN      
        1 gtctacagtc tggaacttgg aaaacgttgt ttctccaagt tcttttttta atttccaagt
       61 aactgaaagt tacttttgtt atttaacatt taggtcaatt ttagacagtt tttgttcaaa
      121 aagcgatatt tatagtggac ggtattactg atatattaag gatataaaat gaaagcgttt
      181 aaaaaggtgg cgtaaagatg attattaaag aagagatttt tgtacctaaa gaattagtga
      241 aagaaaaaat tgacctgctc gttgaaaacc tgacaacgat caaagacgat aatggtgaat
      301 ttttgttggg ctttgatgga ctgaaagtag atgacaagag ctggacagtg tggaattggc
      361 cacaaggtgt cggtctatat ggtatttata aaaattacca gatgacaaaa aatcctcgcg
      421 cttatcaagt ggtcaatgaa tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa
      481 acgtcaatac aatggcaccg ctattgacta tggcttactt gtatgaagac acgaaagatt
      541 cacgctattt gccttattta gaacaatggg cagaatgggt catgaatgac atgcctcgga
      601 caaatgaaga cgggttacag catgccacat atggaccgga aaataagaat cagttgtggg
      661 acgatacatt gatgatgaca gtgcttcctt tggcaaaaat cgggaaactg ctggttgacg
      721 attgctattc cagagatcat tgaaatctta gaattatcga aagaagatgc attgcgcaaa
      781 ttattgatcg aaacacttga agcacaagtg cgtgcattaa aaacttatca gtctgaaagc
      841 ggtttatggc atacgttgtt ggatgatcct tcttcttacg tagaatcttc tgcaactgca
      901 ggatttgctt acggtatttt gaaagcggtc cataaacgtt atttaccaca agaatataaa
      961 gaagtcgctt atcgtgctat ccaaggattg cttgaacaaa tcgatgaaaa aggagaagtt
     1021 caaaacgttt caatcgggac aggaatgggg gacagtttag atttttatcg gaatatcgga
     1081 atcacagcaa tgccttatgg acagtcgtta acggtgcttt gtttgacaga attgctcgtc
     1141 tcttattgtt aaaagaaagg aaacccatat gaaaaaaaca tcactgctgt ttgcagcaag
     1201 ttcgcttagc ctaggtctgc taggtctaag cggctgcgga aataattcta gtgtggcaac
     1261 tgtcgcagat gcagacgtaa atgagatagt taccttgcgc tttgcttatg caagcaacag
     1321 ccagtaattg atgcaatgaa tgaatttggg cgtttagtaa aagaaaaaac agatggttcc
     1381 gtacagatcc agtatttccc agatggacaa ctgggtggag aacgtgaact gatcgaattg
     1441 acacagtcag gagcagtaga tttcacaaaa gtaagtgcct cagcgttgga aagtttctct
     1501 aaagattatt cgattttttc cgttccttat ctattcaatg atgaagaaca tttctttaag
     1561 gtgatggata atgaagaaat aatggagcca atctaccaat cgaccaaaaa tttaggattt
     1621 accggattga cctattatga ttctggtcag ctcagctttt atatggtaga cggtccgatc
     1681 catacgccgg aagatttgaa gggaaagaag atccgtgtga tgcaaagcga aaccgctatc
     1741 aaaatggtag agcttttagg cggttcgccc gtacctatgg gaagtgatga agtgtacacg
     1801 tctcttcaat ccaatttgat caacggatca gaaaacaatg aatttgtctt gtatacagct
     1861 ggacatggcg gggtagctaa atactattcc tatgatgagc atacacgtgt gccagatatc
     1921 atcatcatga atgatgccat caaagaacag ctaactgatg aacaggaaaa agccattgaa
     1981 gaagcagcga aagaatcaac ggtttttgaa attgaagctt ttgcaaaagc aattgaagaa
     2041 gaaaaagctt tagcacaaga agaacatgga gtccaattca atgatgtaga taatacaccg
     2101 tttcgagagg cagttgcccc acttcatgat atattcaaaa acgatcctac ctacaaagac
     2161 ctttatcaaa agatccaaga gcaaggagcg tagctgtaat gaaaatgatt ttaaacaagg
     2221 tattagagat cctgggagct gttttgcttg ttgggatggt cgctgttgtt ttatggcaag
     2281 tattttccag aacggtttta ggaaatccaa atacggtcac ggaagagctt gttcgattcg
     2341 gtcttgtctg gtttgccatg ttgtcttccg cgtatgttgt gggacaaaaa gcacgtttag
     2401 ccgtaacggt tttaagtgaa aaattaactg gcaaaaaaag taaaatttta gaagttgttg
     2461 ttcaaagttt atttctggta tttgctgcga tgatcatggt gtatggcgga tggaatgctg
     2521 tcacattgac aatggggcag atctcgcctt ctttaagtat tcctatggga tatgtttatc
     2581 tgtcagtacc tgtatcgggg gtactcatca ttatctatag cctgatcaat ctaatcgata
     2641 cattaagcaa caagccagca attcaagaaa aaatataaaa atggaggaat aaaatggcaa
     2701 tagaagccgg attagtgtta ttttttgtat ttgttgtgtt gcttgtatta gggatgccga
     2761 ttgctataag tattgcattc tcatcgatga tgactttatt gttagttata ccgtttgatg
     2821 tttctgcttt tagttcagct caaaaaatgg tgggaagtat caatagtttt tcgctgatcg
     2881 ctattccgtt tttcgttctc tcgggcatta tcatgaataa tggagggata gctaagaaac
     2941 tagtggattt tgcgatgttg ttcgtcgggc gcgtgccggg ggctttggct catacgaatg
     3001 tgttagggaa tgctttattt ggttccatct ctagctcggc cattgccgct tctaccgcga
     3061 ttggtggagt gttgatccct cagcaagtgg aagaaggata tgaccggaag tttgcgacag
     3121 cggtcaatat cgcgtcagct ccgacaggta tggtcattcc tccaagtact gcttttatca
     3181 tgtattcact tgttgctggt ggtgcttcga tctctgcttt gtttatggga ggttatctcg
     3241 taggcg
//
LOCUS       DACEMK010000164         3236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEMK010000164 DACEMK010000000
VERSION     DACEMK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.171"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            204..1172
                     /locus_tag="IW715_002604"
     CDS             204..1172
                     /locus_tag="IW715_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1023178.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IW715_002605"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IW715_002605"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1023179.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IW715_002606"
     CDS             2202..2810
                     /locus_tag="IW715_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1023180.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          964 a          616 c          785 g          871 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaagat gtaaggcaga gcgatattgc
     2941 ggtcattatc tgcgtgcgct gcggcaagat agcctgataa taagactgat cgcatagagg
     3001 ggtggtattt cacaccgccc attgtcaaca ggcagttcag cctcgttaaa ttcagcatgg
     3061 gtatcactta tgaaaattca tctacattgg tgataatagt aaatccagta gggcgaaata
     3121 attgactgta atttacgggg caaaacggca caatctcaaa cgagattgtg ccgtttaagg
     3181 ggaagattct agaaatattt catacttcca actatatagt taaggaggag actgaa
//
LOCUS       DACEMK010000165         3209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEMK010000165 DACEMK010000000
VERSION     DACEMK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.172"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            116..976
                     /locus_tag="IW715_002607"
                     /pseudo
     CDS             116..976
                     /locus_tag="IW715_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="IW715_002608"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="IW715_002608"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ1023181.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="IW715_002609"
     CDS             1830..2678
                     /locus_tag="IW715_002609"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ1023182.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="IW715_002610"
     CDS             2688..3083
                     /locus_tag="IW715_002610"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ1023183.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1246 a          326 c          642 g          995 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttat
//
LOCUS       DACEMK010000166         3152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEMK010000166 DACEMK010000000
VERSION     DACEMK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.173"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(53..2545)
                     /locus_tag="IW715_002611"
     CDS             complement(53..2545)
                     /locus_tag="IW715_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1023184.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2552..3019)
                     /locus_tag="IW715_002612"
     CDS             complement(2552..3019)
                     /locus_tag="IW715_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1023185.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          835 a          694 c          517 g         1106 t
ORIGIN      
        1 cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt aactataggg
       61 aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct tttgaagcac
      121 ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg ctcaatcgat
      181 cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca ggatcaaatc
      241 cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata tcttgttctt
      301 tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt tcatctttac
      361 tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt cggaaagctt
      421 tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta acatcttgga
      481 cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa
      541 tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa tgaccatcat
      601 ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact tcatctaaga
      661 gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct tcatcatacc
      721 ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc ataaattctg
      781 acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa agtgctttgg
      841 ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca attggacgat
      901 caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct
      961 catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag cgttcgcttt
     1021 cttttttctc caactgctgt agagggacac cagtccactc cgatacaact gtagcaacat
     1081 cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt gcagaaactt
     1141 tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt
     1201 cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct agtttgatca
     1261 cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct gattcatcga
     1321 tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca gaaagattga
     1381 ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca tatcttgtac
     1441 gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact tgaatgcgag
     1501 caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca tctagggtcg
     1561 ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata ttcgaagcat
     1621 caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca atgaataaga
     1681 ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct tcaaattcac
     1741 cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg cgcttttctt
     1801 gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca gcaatggctg
     1861 ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt cggctaagaa
     1921 tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc ttgttttctt
     1981 ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt
     2041 gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca gcagaatcac
     2101 cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg atacctaagt
     2161 tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc aaaatatgtt
     2221 ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa gcaaaaactt
     2281 gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg ataccaccat
     2341 aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc atttcacgta
     2401 atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca tgttctgaac
     2461 taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat actgcttggg
     2521 ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa cttaaacgtt
     2581 ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg aaattcaatg
     2641 tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt atttggtctt
     2701 cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca tcaaataact
     2761 gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca attcgtatat
     2821 aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta aaacgggtat
     2881 taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt tcaatccttc
     2941 gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca attaaatccg
     3001 aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct taattataac
     3061 gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt tagaatcaac
     3121 aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DACEMK010000167         3138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEMK010000167 DACEMK010000000
VERSION     DACEMK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.174"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(862..1203)
                     /locus_tag="IW715_002613"
     CDS             complement(862..1203)
                     /locus_tag="IW715_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1023186.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IW715_002614"
     CDS             complement(1203..1433)
                     /locus_tag="IW715_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1023187.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IW715_002615"
     CDS             1720..2058
                     /locus_tag="IW715_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023188.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IW715_002616"
     CDS             complement(2263..2502)
                     /locus_tag="IW715_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1023189.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IW715_002617"
     CDS             complement(2478..2825)
                     /locus_tag="IW715_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1023190.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT          994 a          573 c          486 g         1085 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattccgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaa
//
LOCUS       DACEMK010000168         3009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEMK010000168 DACEMK010000000
VERSION     DACEMK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.175"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(25..144)
                     /locus_tag="IW715_002618"
     CDS             complement(25..144)
                     /locus_tag="IW715_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1023191.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IW715_002619"
     CDS             complement(159..1343)
                     /locus_tag="IW715_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1023192.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="IW715_002620"
     CDS             complement(1602..1892)
                     /locus_tag="IW715_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023193.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3009)
                     /gene="dcm"
                     /locus_tag="IW715_002621"
                     /pseudo
     CDS             complement(1882..3009)
                     /gene="dcm"
                     /locus_tag="IW715_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          812 a          722 c          533 g          942 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataa
//
LOCUS       DACEMK010000169         2992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEMK010000169 DACEMK010000000
VERSION     DACEMK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.176"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1053)
                     /locus_tag="IW715_002622"
     CDS             complement(106..1053)
                     /locus_tag="IW715_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1023194.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IW715_002623"
     CDS             1299..1763
                     /locus_tag="IW715_002623"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1023195.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IW715_002624"
     CDS             complement(1825..2868)
                     /locus_tag="IW715_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1023196.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          990 a          593 c          571 g          838 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACEMK010000170         2984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEMK010000170 DACEMK010000000
VERSION     DACEMK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.177"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..644)
                     /locus_tag="IW715_002625"
                     /pseudo
     CDS             complement(1..644)
                     /locus_tag="IW715_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(718..999)
                     /locus_tag="IW715_002626"
                     /pseudo
     CDS             complement(718..999)
                     /locus_tag="IW715_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1461..2843
                     /locus_tag="IW715_002627"
                     /pseudo
     CDS             1461..2843
                     /locus_tag="IW715_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1033 a          529 c          506 g          916 t
ORIGIN      
        1 cagaccgaaa acccgaagag agtgccttct tttcgggttt tcttatataa tcctcgaatg
       61 gcttccatgc ctttaatcgt ggtagaggca gtgcgtaaac ttcgatagaa tttattgcgt
      121 ctctttactg gacgatggtc ttgttcaatc aaattattca ggtatttaat ggtacgatgt
      181 tctgtccctt gataaaagcc gtattctttt agtttcttaa aggcacttgt aatagagggg
      241 gctttatctg tgactacaac cttcggttca tcaaactgct tcactaaccg cttaagaaaa
      301 gcataggctg cttgtgtgtc ccgtttttta cgtaaccaga tatctaaggt taagccgtcc
      361 gcatcaattg cacgataaag ataatgccaa cgtcccttaa ttttgatata ggtttcgtcc
      421 attttccatg aatagaagga ttgtctattt ttcttcttcc aaagataata gaggactttg
      481 ctgtactctt atacccaacg ataaatcgta gtatgacaaa catttattcc acgatcatat
      541 aacaattcct gaacttcacg atagcttaga ttgtaacgca ggtagtaacc aacagcgaca
      601 ataatgacgt cttttttgaa ttgtttgcct ttaaaatgat tcattactct gtcctctctg
      661 tcttttttct caattttaca ctaaaataga ttttttggaa aactttgcaa cagaaccgaa
      721 atgagtatag tatgaattag taatttggtt ttcatctaag tttagctttg atttactaaa
      781 attgatatct cgatacatgt tcaatacatc taatacaaat cgagaatctt ctttcggaaa
      841 tggctcagaa agatcactcc aaatttcatc atcataatga tactcgtaac cttctctcaa
      901 tatttcgatt tttctttcat aataagatct gttttggtcc tcaaatttag ccaataactc
      961 aaattgattt actagtgaca atctctgaaa actgtccatt aaatgtttct tcctatcttg
     1021 ttcattaaca aaaaattata agcccttaca acgaatatta tatattaaat ttaattgtag
     1081 gtgaacacga attttataaa tatttatggt gttaatgaca aatgatatcc gactcctctt
     1141 catacgttct ggactatttg ctaatagatt aaaatttcag catctatttc tgaccataaa
     1201 taaatgagct gccgaaaagc tgccaaatca gacttgaaaa taaaggatca aaaactttta
     1261 agtttctgac cccgtaactc acacttcaaa atcttactta aaaaggagtc tgaaagaaaa
     1321 aaagagtatt caaaattttt ttctaactag ctacatcaca tctatgcatt gacactcaaa
     1381 gaaccagaat aagtcatcag aaagcaaggg ggtgcggaca taatcctgga cgaaaaaata
     1441 aacttaggag gaatgtccgt gtgttttccc acaaagagcg tgtaagggca attcagttat
     1501 ttttaaaata cgattgctct tatgctgcaa ccattcgaga attagggtat ccgtcgattg
     1561 gtgcactacg gaaatggtat aaagagtatt tggaatccgg agagctccat caggagcacc
     1621 gaaaaaaatc aaagtattct gaagaacaaa aacgaatagc ggtgaatcat tattttgaat
     1681 atggtcaatg ttatgcacga accattcgca tgcttggtta tcctaataga gagtccttgc
     1741 ggcagcggtg tgaagaactt gcaccaggag ctagaaaact ccgaaaaagt gcggtaaagt
     1801 taacacaaga tcaaaaagaa gctgtgttga aaaaattcta taaaccgcaa actaatagga
     1861 aaaatttagc cgaatctgga ggaattagta gagttactct atatcaatgg aaaaacacgg
     1921 ttcttggaaa ggattttcca ttgtgtatga ctttaaaaaa tcaggaaaaa caaaaagagc
     1981 tacttttaaa ggaagtagaa gacttacaaa aacaagtaca ccaattacag ctcgaaaaag
     2041 cattactcga aggagcggct gaactgctaa aaaaagaaaa aggcgtcaat cttctttgtc
     2101 tatcaaatca agaaaaaacg atattgattg atgccttacg aaatcagttc accttgaaag
     2161 agcttcttca acagctccag tttcctaaaa gcagctattt ttatcaaaaa caagcacttg
     2221 agaaaccaga taagtattac aaagaacgtc aaattaatta tcacgatttt caatcataac
     2281 ttctgtgcgt atggttaccg tagaattcac caagctttaa aaaatatggg taaaaagctt
     2341 tctgaaaaag ttgtacgtag attgatgact gaggaaaatc tatttgttaa attttctaga
     2401 cgaaaaaagt attcttcgta tgctggggaa atttctcctg cacaccctaa tttattaaat
     2461 cggaatttta aagcaaagaa gccaaatgaa aaatggttaa cagatattac tgaatttaga
     2521 attctcgcag ggaaaatcta cttatcacca cttgtcgatt gttacgatgg tgctattgtt
     2581 agttggacta ttggaacaaa gccaacttgc ttcactctat gtctaaaaag ggttattcac
     2641 cagataattc agcgtgcgaa ggtttctttg gacgattaaa aaacgagatg ttttatcaac
     2701 gtgactggaa gaatacaaca atcaatcagt ttataaacaa attggatgat tatattcatt
     2761 ggtataataa tcaacgaatt aagttatctt taggagggct aagccctctt caatatcgga
     2821 agaagcaaag atatatttgt taaaaaggtc caaaaaaaat atccgcaccc cctttttttt
     2881 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2941 gcaagaactt atctcattca aggcttgatc ttccaagtaa aaca
//
LOCUS       DACEMK010000171         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEMK010000171 DACEMK010000000
VERSION     DACEMK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.178"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(59..421)
                     /locus_tag="IW715_002628"
     CDS             complement(59..421)
                     /locus_tag="IW715_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1023197.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            644..1396
                     /locus_tag="IW715_002629"
     CDS             644..1396
                     /locus_tag="IW715_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1023198.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1536..2459)
                     /locus_tag="IW715_002630"
     CDS             complement(1536..2459)
                     /locus_tag="IW715_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1023199.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2474..2746)
                     /locus_tag="IW715_002631"
     CDS             complement(2474..2746)
                     /locus_tag="IW715_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023200.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT          940 a          557 c          477 g          944 t
ORIGIN      
        1 ttttattgta tacattagat atggagcagg ttaccataaa gatgattatt agtcgttatt
       61 aaatagaatc cttcgtattt ttttgttcta tcactacaat atctttattt ggacgtttga
      121 attgtattcc ggcacgttct agctcctgtt tcactcctac atggactttg acatcttctc
      181 gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg atttttttct
      241 tcgtattgat catcacgact tgctttttat catatgtgtc taccaatagc agttgtgttc
      301 gatctaacga aaataatttg taataattaa tagccgtatt cccaaaaaag aattccacta
      361 ctttaggata aaatgtctta atatttaaat tactattcac aggtgtttcg attagcttca
      421 tctcattcct cttttctata tagtcgattt agatccaatc ttaagttatg ttaccatctt
      481 tcctagcatt aaggaatcgg ttacaattga acctttaaag acaaaaggat ttacctttta
      541 tcacttaata gtataacata atttcgtgag aaaaaaatac aattttttct tgaccatctc
      601 gttacgtaaa ggattatgct tacatttgtc aggaggaaaa ataatggagt acacaataaa
      661 gaaaatgtca gagatatcag gagtcagtcc gagaaccttg cgcttttatg atgagattgg
      721 actgctaaaa ccagctagga tcaattcttc aggttatcgg atttatggca aaaaagaagt
      781 cgatcgtctg caacacatct tattttatcg aacaatggca ttcaagttgg atgatatcca
      841 agaggtctta gacaatccat cttttgacca ccagaaagct ttaatcaagc atcagcaaat
      901 gttgctggaa aaacgggcac aaatcgatac cctcttgaca actgtccaac agacacttga
      961 tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt ttaaacaaca
     1021 aaagttgaaa gaaaatgaag aatcctatgg acaagagatt cgacaaaaat acggaaaaaa
     1081 aaaggttaca gcatccaatg aaaaatggat gaatatggat aaagaaacgt atgaagaaat
     1141 gcaaactgtt gaaaagcaga tgttatttga tctcactctt tacttaaaag aaccagaaga
     1201 tgaaagctta gcagaccgta tcttccaagc tcataagaag tggttctctt acagctggcc
     1261 tgaatatcag ccaaaagcgc ataaaggact aggaatgatg tacgaagccg atgaacggtt
     1321 cactgcatat tatgacgagc gaagtggtca tggtgcagca aaagctttaa atgaaattat
     1381 ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa aatgacctaa
     1441 taacaaaaca gcagacaccc aagttagatt tttatgctaa cttttggtgt ctgctccatt
     1501 ctttcacaag ttttttcatc ctactttaaa tatattcatc gttgaataaa cgtataaggt
     1561 attttcactt tatccttttt ttctgtaaga aacaattgaa atgccatttc tccgcaagct
     1621 tccagatggt ggtcgatggt tgggaaacca agaagctcgc tgatcagcaa attatcgcgt
     1681 ccaatcacca ctggtgtctt atcttgcaga tagctttgca agatgcctgc tgcaaccata
     1741 tccgaatttg taaagacacc atcaactcca agctctttaa aaaagaagcc tgcttgtttt
     1801 cctttctctg catctataca atcccaaaaa acaagtgatt catcaaaaga tggaaaaagt
     1861 tgatgacata gctgcaaagt gatttttgag ttataactta gcctgccact tcgccccaac
     1921 gtgatgccca attgctttat accttgctgc ttcaaataag taagaccttc tgtaattgat
     1981 tgttctcgat caataaagac acagcttgcc tgttttccct tgatttcttc acaaaaaact
     2041 agaggtccat atttttgata tgccaataaa tcatctagtg tattggcacg agacgtaatg
     2101 atcaatccat cgaaagcttt agctgcaaat tcttcaagat accttcgctc tttttcttta
     2161 tgatagcccg tcggtaaaag tgtcactttg taatcataat caaacgctgc ttcaatgatc
     2221 ccactgatca actgatcaaa atacggatga ttggtataag gcagaatcac tccaatgctt
     2281 ttcgtttttc cataacttaa ctgtctggca tttgcattag ggatataatc gacttctttc
     2341 atgaccgcaa gtatttcttt ccttttttcc tcatctacat atggatgtcc attgattaca
     2401 cgtgaaatag tagaaactga ataacctgtg agctttgcga tatctcgaat gtttgccact
     2461 gctgcttcgc cacctataca aaaagatttt cttgtaaata gtctaccaca gctggaccgc
     2521 attcaaaaaa ctgatacatt tttccttcat gcgaaaaact gatccgctgc ccgccccaat
     2581 atatatcttt tgtcaaattt tcgatttcat tcattggtat ttcttttact atattcctat
     2641 tcaacccttg aaaactgtac aacgttaata cggatccctt gaactcgaca aacgcataaa
     2701 aataattgtt cgttgtgcca tctgttatct ctacataagt attcattgca agccctcctt
     2761 ttgatacttt gagtatagtg tatgaaacgc gtctcatagc aaggagtttg ttttgccaaa
     2821 gaaaagtttt attcctcttt cattattacc aaagtctctt attttaaatt gaatacatta
     2881 taatgatact gtaaatatat ctttattatt agtgaatg
//
LOCUS       DACEMK010000172         2862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEMK010000172 DACEMK010000000
VERSION     DACEMK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.179"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..2377
                     /locus_tag="IW715_002632"
     CDS             5..2377
                     /locus_tag="IW715_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023201.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..>2862
                     /locus_tag="IW715_002633"
     CDS             2438..>2862
                     /locus_tag="IW715_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023202.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          455 c          621 g          642 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gc
//
LOCUS       DACEMK010000173         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEMK010000173 DACEMK010000000
VERSION     DACEMK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.180"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW715_002634"
     CDS             <1..197
                     /locus_tag="IW715_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023203.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW715_002635"
     CDS             314..811
                     /locus_tag="IW715_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1023204.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW715_002636"
     CDS             898..1290
                     /locus_tag="IW715_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1023205.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IW715_002637"
     CDS             1274..>2778
                     /locus_tag="IW715_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1023206.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEMK010000174         2627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEMK010000174 DACEMK010000000
VERSION     DACEMK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.181"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IW715_002638"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IW715_002638"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1023207.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          943 a          459 c          581 g          644 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaact
//
LOCUS       DACEMK010000175         2623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEMK010000175 DACEMK010000000
VERSION     DACEMK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.182"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..170)
                     /locus_tag="IW715_002639"
                     /pseudo
     CDS             complement(1..170)
                     /locus_tag="IW715_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            411..593
                     /locus_tag="IW715_002640"
     CDS             411..593
                     /locus_tag="IW715_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023208.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            577..762
                     /locus_tag="IW715_002641"
     CDS             577..762
                     /locus_tag="IW715_002641"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1023209.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            784..909
                     /locus_tag="IW715_002642"
     CDS             784..909
                     /locus_tag="IW715_002642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1023210.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            1005..2525
                     /locus_tag="IW715_002643"
     CDS             1005..2525
                     /locus_tag="IW715_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1023211.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
BASE COUNT          892 a          442 c          527 g          762 t
ORIGIN      
        1 tgttggaaac ctttatgact gcgacctagg aatttttggt tgtaagtatc aaaagacgag
       61 actaagaaac gttccatcga ctcttcattt tggaactgtt ccttgcggtg gctgtatttc
      121 ttgatttgtt tattgaaaga ttcgattaaa ttagttgagt aaatgcttcg tacgaacata
      181 gttgatttat tcaccatact tcccattaca tcgtaaaacg ctatatttaa gatttcaaac
      241 aactatatta ataggaagaa aacttcattt ttcctcttag ttctataaaa agtgtggcta
      301 atttgaggca aaatattaaa ttaaatgtaa gcgtgtcata ggatagtatc tcgtacgccg
      361 attatctttc ttctataatt aaactatcaa atatagaagg agaaaaacga atgaataaaa
      421 aagatattgt accagttcaa ttttcaagtg cggctcaaaa atcgcaaatt gacaccaagc
      481 caaaactagc ttgcagaatc aaatcggaag ataaggaaat atttatttat aatggaatgg
      541 ataaatatct tttgtatacc ttgttaaaag agttgaatgc caatgctcat tgatgcaact
      601 gcattgaaac atatttttat cgtttgtgga aaaacagatc ttcgaaaagg aattgatgga
      661 ctagcagctt tcgttataga agaatatgac cttgatgttt acgatcaagc gcttttctta
      721 ttttgcggta cgaagaaaga tcgcttcaag gctctttact aggatcaaaa tggatttgta
      781 ttaatgtaca aaagatttga aagtgggtac cttcaatggc cgcgttccag aaaagaagtc
      841 ttactgactg gcctttcaat cgaagaaaaa tgtacgctaa aagaaacgaa aaaaggtgcg
      901 atatattaga cgaaaacctt gttaaatcaa ggattttgtc tgtttttcgt tgtcataaaa
      961 tggtataata gatgtaagaa aatcgctaga atggtggtgt aaaaatgaca aaaatggaat
     1021 ctgaacttct tgtacaaatc aagcaattaa cacagttgct tgaaatccag caacaggaaa
     1081 atactcttct tagagaacaa attgctgaaa tgaatcgacg cttgtttgga cgaaaaaaag
     1141 aaacaccacc cgtagatggg caaatcgatt tattagacga ctcaactttt aatgaaccag
     1201 agcacactgg acaagaaagc caggaaccca ttacggtatc ttcttttaaa cgcagaaaac
     1261 gcaagggact caaagcactt tcattagaag gtctgcctga agttcctatt cattatgaat
     1321 taaaaggtgc cgattgtttg tgtgaagact gtggtcatac tttacatgat atcggtgcga
     1381 caaggcttag agaagagcca ttgtatgttc ctgcccatat agaaaaacaa gtcatctacc
     1441 aacatgccta tcattgcaga gaatgtgaag cattaggaga aacaaaaatc aaaaaagcgc
     1501 ctgttcctaa accgttgatc aataatagtc taggttcctc ttcaattgtg acacagagta
     1561 tttgggaaaa atttgttaaa aaggtgccgg catatcgaca agaaaaagaa tggcatgcac
     1621 ttggtttccc tcttgatcgt aaaaaaatca ctaattggca tatcaaggta agtgaaatgg
     1681 ttttagagcc aatctacaat tgccttcatc aagaattaag caatcaggag gtcttacacg
     1741 cggatgagac aagctatcgg gtattgaata ctcataaaga aaagaccttt tactggttat
     1801 atgcttcagg taaaagcgaa gaaaaacaag ttgtgctcta cgagcacgca aattcgcgtt
     1861 ccgttgatga gccaaaaagt tttttagaat catttagcgg tttcttgcat actgatggtt
     1921 acgcggccta tggaaagctg ccggtaaaca atgtttattg ttgggcgcat gttcgcagaa
     1981 agttttttga agcattgggt aaacaaccgt ccccaaaaag tcatgccaaa ataggattag
     2041 acttctgtga caagatgttt cgaatcgaac ggacctttaa taagcttagc gcagaagaaa
     2101 ggtttgtagc gaggaaggaa acgttgcagc ctgtgatgac tgaatttttt cattggtgtg
     2161 agagttttgc ttatttgcca aaatcgcagc ttggaaaggc ggttcaatac gcagtcaatc
     2221 ataaagacgg acttcagatt gtgttgcttg atggacgctt ggaactttca aataaccgag
     2281 cagagcgtgc catcaaagag ttagtgatcg gtcgaaaaaa ctggctattt tctaaaagct
     2341 taaaaggcgc tagatcaaat ggaattatat tgagtattat ccagacagct gttgcgaatg
     2401 ggttaaacat ccgcaaatat ttaaatcatc tttttacaga gattcccaat ctgtcttcga
     2461 tgacacctga agcgctacgc gcttatttgc catggaatca gcagatacaa gaaatctgta
     2521 aataatataa gccacaaata acctgatttg agtgtatcaa atcgggctat ttgtgtttag
     2581 gtaccattct atgacacgct tacgctcaaa actactgaat tat
//
LOCUS       DACEMK010000176         2580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEMK010000176 DACEMK010000000
VERSION     DACEMK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.183"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..662)
                     /locus_tag="IW715_002644"
     CDS             complement(<1..662)
                     /locus_tag="IW715_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1023212.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TD"
     gene            complement(982..1389)
                     /locus_tag="IW715_002645"
     CDS             complement(982..1389)
                     /locus_tag="IW715_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023213.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(1408..2226)
                     /locus_tag="IW715_002646"
     CDS             complement(1408..2226)
                     /locus_tag="IW715_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1023214.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          779 a          392 c          352 g         1057 t
ORIGIN      
        1 tggtctgttt cagtgtcttt taagtgtctt ttagtgtcta aagatttatt aatataataa
       61 tcttgtgcga tttccgcatt tccagtgtta tcaagggttt cagctgtttt ttctcgacgt
      121 gcgatttccg catttccagt gttatcaagg gttttagcag tttcctctcg acgtgcgatt
      181 tccgcattcc gagaagaggt tgatttatca atagtttcgc ttaaatcgtc atcgaaagaa
      241 ttttgaataa aaagatcatc ggcacttatt tcaggttgaa gtaaatagta tttatttttt
      301 tcattttttt tcgtttttgg gttaaattct ccttttacga ctgaaatcaa attatatgtt
      361 tctaactcgt ttaaaatggt ttttactttg ccttggtagc aatttaattt gtccattagg
      421 tctgcttgtg taaattccaa atataataga ccatcatcgt cgatccattt ttcatgtaag
      481 gctctacgaa attcattttt aaaaagcata taggctattt ttgagctgtc tttcatttta
      541 gagtatttag aattctgaat taaaaaacta ggaaatttat aatatttttc aatataaaca
      601 ttcgttgctt tatatcccca atttttaatt atcatttttt gagattgatt ttctgtagac
      661 attaaatttc tccttcttct tagaaacttg tatatattca ggattttcaa caagtgaact
      721 ataccatgta ttaagggtga ataaaacact ttcagtattt aaggtataag ttatatatta
      781 aaaaaataag tataagttat actttaaata caaataatgt ataacttata ctttaaataa
      841 taattttata atgttgattt aatcgcaaat ttacacgttg tttattaagt tataccttaa
      901 atatttaatg tataacttat actttaatat tcatttatct agtataactt atactttaat
      961 tgaataccgc atgaaaaaaa attattttgg taatttttca aactcttctt gtatttcttc
     1021 tcttatgtgt tcttcttttg ctactcctag tctatgctgg atatatgagt ctgctaatgt
     1081 attaacaatt ttttcgaaag aaattctccc tttttcactt tgtaactccg tcatatagtc
     1141 ttgtaaaatt gagagtttgc tatttaatgc cggagagatc tttttaggaa ctgatacctt
     1201 gtttctttta tcaggaattg aaactggtcg gcctcttttt ttcaatgtaa tatttcttgt
     1261 gttactcaat gcactattgc taaagttata ttcaccgctt attttcacat cttggcgttt
     1321 gttattgggt aataatggct caaattcacc attctccatc attttaggaa tatcaaaatc
     1381 tttaggcatt acttatgcaa ctccttatta tttatttaaa aactcttgtc tagcatctag
     1441 ttctgccaaa atatttataa atacttggtg tgctttataa tcccattgat caaaatttcc
     1501 atttttgtat tttcttaaat gtataccaat tttagaatac cttctcaatc ttttttgata
     1561 atgaatgaga tttttggata ccacggttcc gtatttttca attgcttctt ctaggatttc
     1621 ttcatcaaaa tcatcatttg gttctaccat acaaggtaca atatctataa tttgcaaatc
     1681 aagatcgtat ctatcgacca tgaatttttg ataatttaca tatttgttga ctccctctaa
     1741 ggattcttca gctgtttgaa atgaaattat cgaatagtct gaagctgcca tagcattatc
     1801 ggaaaattcg ctaatggttg gtggtacatc aataaatatt tcttgataat tgttctttat
     1861 tggaagaagt aattttttca agacagtaac ttggtctaat tcattttcat gtttattagc
     1921 aaaagcggag aatgatcgaa aactagtgtc acatgccaga aggtcaagat tttcatttac
     1981 cttaataata ctacttgcta attctccatt tgcgacacca tttgttatag tggttttgat
     2041 atcggttata ccgaaagttc ttgctatgac ttgtgttaag ttcgcttgag ggtctaaatc
     2101 aataataagc gtttttctat ttcttatcat tgcattgtca taagcaagca tgctgactat
     2161 cttagtttta cctactcctc ctttaaaatt gccaactgta aatgtagtcc cttgaaaagt
     2221 atccatgtaa atcctcccaa tctttaattg ataattaaag tataacttat atatcggaaa
     2281 tggtcaacat taaaagtata acttaaataa taatatctaa aaacgtttgg atatagcggt
     2341 ttttaataat aaaagtataa gttataccat atatatttat ggtataactt atacttttat
     2401 tattaaaggt ataacttata ccttaaatat tgcttttcga aaatagctat tctagtctat
     2461 ctgtttcttt acttttcgct gctgatttga attgaattga tcatattcat tttctagtat
     2521 taataacttt tcgtatacat cggtcatatt catactagaa gcaagactta gatcatatac
//
LOCUS       DACEMK010000177         2475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEMK010000177 DACEMK010000000
VERSION     DACEMK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.185"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..418
                     /locus_tag="IW715_002647"
     CDS             <1..418
                     /locus_tag="IW715_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1023215.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="IW715_002648"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="IW715_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1023216.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="IW715_002649"
     CDS             1305..2459
                     /locus_tag="IW715_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1023217.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          854 a          443 c          521 g          657 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat aattt
//
LOCUS       DACEMK010000178         2468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEMK010000178 DACEMK010000000
VERSION     DACEMK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.186"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(493..1785)
                     /locus_tag="IW715_002650"
     CDS             complement(493..1785)
                     /locus_tag="IW715_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1023218.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IW715_002651"
     CDS             complement(2059..2319)
                     /locus_tag="IW715_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023219.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          814 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagt
//
LOCUS       DACEMK010000179         2413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEMK010000179 DACEMK010000000
VERSION     DACEMK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.188"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..342
                     /locus_tag="IW715_002652"
     CDS             91..342
                     /locus_tag="IW715_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023220.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            324..596
                     /locus_tag="IW715_002653"
     CDS             324..596
                     /locus_tag="IW715_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023221.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            763..1164
                     /gene="tnpA"
                     /locus_tag="IW715_002654"
     CDS             763..1164
                     /gene="tnpA"
                     /locus_tag="IW715_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1023222.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            1176..2321
                     /gene="tnpB"
                     /locus_tag="IW715_002655"
     CDS             1176..2321
                     /gene="tnpB"
                     /locus_tag="IW715_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1023223.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
BASE COUNT          915 a          365 c          436 g          697 t
ORIGIN      
        1 ttcctataca atgagacacg tgttccaaaa aaacattata aggaaagatt gtcggtagct
       61 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ttatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggcgtca aaccaagcac aatcaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagctaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatgttac atcatgaagt aatcagacgg attcaaaaag
      361 actttagttg tatggaagaa atgaagtact ttgttcaaaa tgttgaagaa cagttatatt
      421 tggaaagcga aacgagtcaa acaaaggtca cagtcagcca taatcgttta gatgtgtata
      481 aacgtcgtcc tgaatcatta aaaaaaggag cggagcttct acgactagaa acagaaaaat
      541 acgtcatgaa tgtttaccta gaaaaaagtg agttggtcga aaattttatg gactagccga
      601 gaacacccgt gattttaatc atgggatgaa tcggattcag ttgtgaccta gctttagcta
      661 ggttaaacaa ctgacaaata taaactaaat aatagtgaaa tgtcttgcaa taattgattt
      721 tgtagaaata catatattaa aataattgcg aggtgttttt gtatgtcaga aattcattat
      781 ggacggggtt atgtctattc tattcaatat catcttgttt ggtgtgtaaa atatcgacat
      841 gatgttttgt atggtcaggt agatattgat gtcaaacagt ttttgaaaca aattgctgat
      901 gataataata taaaaattat agaaatagaa tcagataaag accatattca tttactcatt
      961 gaatgcaccc cacaacacta tattcctagt attgttaaag tatttaaagg cgcatccgct
     1021 agactgctgt ttaaaaaaca tcctgaactt aaacaactcc tttggggtgg acatctttgg
     1081 aatccaagct actttgtagc tactgttagc gaaaatacag aagaacaaat tagaaaatat
     1141 atacaaaatc agaaaaagaa atgaggtgaa tccgtatgct acaacatagg gcttataaat
     1201 ttagaatcta tcctaatcaa gaacaagaaa tattaatgac taaaactatt ggctgttcaa
     1261 gatttgtcta taattatttt ttgaatttgt ggaatcatga atatacgact actggaaaag
     1321 gattatctta taattcttgt tctgccatgc ttccacaaat gaaaagaaat gcagagacaa
     1381 tttggttaaa agaagttgac agtattgcga ttcaatcctc attgaaaaat ctttctgatg
     1441 ctttttctcg cttctttaaa aagcaaaata aacgaccaca atttaaaagt aaaaaacatc
     1501 ctatacaaag ttatacaact aaaaatgtga ataatagtat cgaaattaga aacaatttcg
     1561 taaaactacc taagttagga tttgttaaat ttgcgaaaag tcaagagccc aaagggcgaa
     1621 tcttaaatgc tacgattcga aaaaattcga gtggtaaatt ttttgtttcc atcctgtgtg
     1681 aagaagaaat agttgagttt cctaaatcta attccgctat tggaatcgac cttggtataa
     1741 ctgattttgc aatcctttct gatggtcaaa agattgataa caataaattc acgtcaaaaa
     1801 tggcaaaaaa actcaaacgt gaacaacgta agttgtcaaa acgtgcatta ctagctaaaa
     1861 agaaaggcgt caatctcttt gaagctaaaa attatcaaaa acaaaaacga aaagtagcac
     1921 gactacatga aaaagtaatg aatcaacgca atgacttttt aaacaagttg agcacagaga
     1981 ttatcaaaaa ccacgatatt atctgtatag aagacttaaa tacaaaaggt atgttgcgta
     2041 atcataaact agcaaaaagc atttctgatg tgtcttggtc tagttttgtg actaagttac
     2101 aatacaaagc taactggtac ggtcggaaaa tcatcaaagt agataaatgg tttccatcta
     2161 gtcaaatctg ttcaaattgt ggacacaaag acggcaagaa atctctagaa attcgagaat
     2221 ggacttgtcc tgtttgtcat gctcatcatg accgagatat caatgctagt atcaatattt
     2281 tgaccgaagg tctaagatta catgtcatgg gattagctta gatccaatca tcaaaaaccg
     2341 taggaactac ggggatagct tggtaaataa gagaaaccgc tgtgagtaaa gaaatacgct
     2401 tgcaagtatg ctc
//
LOCUS       DACEMK010000180         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEMK010000180 DACEMK010000000
VERSION     DACEMK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.189"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..2068
                     /locus_tag="IW715_002656"
     CDS             206..2068
                     /locus_tag="IW715_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1023224.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="IW715_002657"
     CDS             2068..>2399
                     /locus_tag="IW715_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1023225.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACEMK010000181         2397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEMK010000181 DACEMK010000000
VERSION     DACEMK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.190"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            431..1471
                     /locus_tag="IW715_002658"
     CDS             431..1471
                     /locus_tag="IW715_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1023226.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2295..>2397
                     /locus_tag="IW715_002659"
     CDS             2295..>2397
                     /locus_tag="IW715_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1023227.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQS"
BASE COUNT          898 a          319 c          388 g          792 t
ORIGIN      
        1 ttattgtatt ttttagtagt atataatcta ttgttttcta gcctaaagcc ttgtcttata
       61 agaataatat aatatatact actgaaataa ggtagtatat attactagta atggtagtat
      121 gttttatatg cataaggtag ttcttaattt attttttgtt ttgacactgg tagtacttaa
      181 ttatctttaa ttttaaaact aaattttatt catacgtttt tctggtagta tttaattata
      241 aataatattt atttaaagta gttcttaatg actttaaagg tagtacataa ttcattttat
      301 ttctgatttt aggtagtatt taattaaaaa acagttttaa tgcgtttgca ttttttattt
      361 atctttgcta ttttcgaaaa gagtaggtaa ttttacataa ataaaacaac gtgaaagtag
      421 aggatctaca atgactgatt ttaaattttt taaggcggat agagtttaca acgaattatt
      481 ttatcaattt ccaaaagtct ttattgtttc tgacgaatac aaaaaaatga aagattcaac
      541 taagattgcc tatatgcttt taaaagcaag attagagatc gcaatcagca aacggcaaat
      601 cgatgaagaa ggtaatgttt attttactta tacgacaaat gaactatgta gagtattaaa
      661 ctgccaaaaa caaaaagcga tagcaatcaa aaaagagttg gaatcctttg gtttattatt
      721 acaaaagcag atgggattta acaaacagtt agggaaaaat aatcctaata gactatatct
      781 agcagaatta aaagtctcag aaaatgatat ctacttactc gaaaaatttg atagagagaa
      841 tagggaaaac gttgataaat cagagggtat gaaaatcata cccaccctcg acgaaaaatc
      901 agacgctgaa tcccttgggg ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga
      961 taaatcagag ggtatgaaaa tcataccaga acttaataat aatatattag acactaatag
     1021 acacaatata gacactgaaa aagaccgcct acaagatcaa ttgttgttag acaattttga
     1081 gacaattatg acaaacgaca gcattgctac gtttgtccct gaacgagtat taaatttgat
     1141 aaaaacattt tcttcaagtt acagtgaagc tcaaaaaacc gtccagacta ttcataatgc
     1201 aaagaaaaaa gctgaaatag aaagtggtat ttcgatagtt tttgaagaac tcgatagtta
     1261 ttatgtcaat gcagaacaag aattatacac gacactgtta aaagcctatc aaaaattaaa
     1321 aaccgaaaaa gtcgaaaata tccagaacct gatttttgtc tatgtaaaaa attggtttat
     1381 cgaaaaacca atagctgcta aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc
     1441 aagcactatt acgaaagact ggttagagtg aaactttact gacaaagtga ttgttgcttc
     1501 atggagtaac attaaaatta aaaatttttt attattctaa ttatattacg aatattacaa
     1561 aataatagct attttaatag aatttataag ttaaaatatt atgataaaaa tcacaaacgt
     1621 agccttttac gtcataagtg ttaaaagaaa taattaattt caaatataga aaagggaacg
     1681 tatgtttgca tactcctttt ttttgagtta taataatacg cgaagagaga taatttcatt
     1741 atttaaacaa gctaaaaatc ttgatattac ttcttttcag gcaatacgaa tattattcgc
     1801 ttacattttt tgtataaatt taattgtctg ctatgcttgt gatgaattga ttttcgttct
     1861 gcacttttgg atgataaatt tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa
     1921 ctcggaaggc tggtaacctt cgtcagcgtt catacacggc attaagattt ggcccttaat
     1981 gcgttaacct ttcagtgtta cggtattacc cgcaatggat ttagtataac agatattcaa
     2041 aagtgatacc actttttttg gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc
     2101 tatgcctaag cacaatgtta taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct
     2161 gaaattagag ggaacgcatg tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa
     2221 atcaattctc tatttttgaa aaataacaag aaaaacttgt taaaaatatt taaagaaatt
     2281 gggtggttta ttttatgtca tatacaggaa ttttgagtct caaagatatt tgtcactacg
     2341 gtaaacggtg taccgctaca gagaaaatca cgaagaaact atcaactggc caaagta
//
LOCUS       DACEMK010000182         2353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEMK010000182 DACEMK010000000
VERSION     DACEMK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.191"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..363
                     /locus_tag="IW715_002660"
     CDS             91..363
                     /locus_tag="IW715_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023228.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            808..987
                     /locus_tag="IW715_002661"
     CDS             808..987
                     /locus_tag="IW715_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023229.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            1046..1402
                     /locus_tag="IW715_002662"
     CDS             1046..1402
                     /locus_tag="IW715_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023230.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          845 a          327 c          447 g          734 t
ORIGIN      
        1 ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt gtcggtagct
       61 gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta ctatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa cgaaaatgaa
      361 taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga aaaaaaggtc
      421 aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa cgcctagtaa
      481 ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
      541 gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca tttgaacaag
      601 attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga tagtgcagct
      661 gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat cttttggcgg
      721 tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt tttcataggg
      781 ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa acggttaccc
      841 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtatggcaaa
      901 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
      961 tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat ataagtgata
     1021 tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt acaaattaaa
     1081 gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc atttgtatag
     1141 agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag ctggggatcg
     1201 atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc tttggcttat
     1261 tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact tagataagct
     1321 aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt tagaaagtga
     1381 cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat tattttatta
     1441 agaatgatgg aatataagga taaggttacc aacataacga tcattacttc tctgatatgc
     1501 tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat actgcaagcc
     1561 ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat atataagcta
     1621 tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag ttatcaaaaa
     1681 gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag taggatttga
     1741 catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg gagataagat
     1801 gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac cgcaactgaa
     1861 gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg ttaccttaaa
     1921 atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga tggggtacgt
     1981 gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat aaatttagac
     2041 atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat tatagtaatg
     2101 attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa aattgagaaa
     2161 tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat gaagcttacc
     2221 aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc tttgaggaga
     2281 tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg cggtggaggt
     2341 aaaggttttt aat
//
LOCUS       DACEMK010000183         2323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEMK010000183 DACEMK010000000
VERSION     DACEMK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.192"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1016..1306)
                     /locus_tag="IW715_002663"
     CDS             complement(1016..1306)
                     /locus_tag="IW715_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023231.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2205)
                     /locus_tag="IW715_002664"
                     /pseudo
     CDS             complement(1514..2205)
                     /locus_tag="IW715_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          670 a          438 c          334 g          881 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc att
//
LOCUS       DACEMK010000184         2255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEMK010000184 DACEMK010000000
VERSION     DACEMK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.193"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..417
                     /locus_tag="IW715_002665"
     CDS             <1..417
                     /locus_tag="IW715_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1023232.1"
                     /translation="NISLVFQNYNLIDYLSPIENIRLVNKSADESILFELGLDKKQIK
                     RNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKC
                     VIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            417..1793
                     /locus_tag="IW715_002666"
     CDS             417..1793
                     /locus_tag="IW715_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023233.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1923..>2255
                     /locus_tag="IW715_002667"
     CDS             1923..>2255
                     /locus_tag="IW715_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1023234.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT          881 a          276 c          405 g          693 t
ORIGIN      
        1 aatatatctt tagtatttca aaactataat ttaatagatt atttatcacc aattgaaaat
       61 attagattag taaataaatc agcagatgaa agtatcttgt ttgaactagg tttagataaa
      121 aaacaaataa aaagaaatgt tatgaaatta tctggtggac agcaacaaag agtagctatt
      181 gctagagcat tggtatcaga tgctccaata atactagctg atgagcctac tggtaatcta
      241 gacagtgtta ctgctggaga aataattaat atattaaaga cattagctaa agatagaaat
      301 aaatgtgtaa tagttgtaac acatagtaag gaagtagcag attctgcgga tatcatttta
      361 gaactaagtg gtaaaaagtt gaaaaaagta aataaaatga atttggaggt tgaataatga
      421 taaaaaatgc atttgcttat gtaactagaa aaagtttaaa atcattaatt atcatattag
      481 ttattttatc tatgtcaact ttaagtctca ttagtttatc tattaaagat gctacggaca
      541 gagcttcaaa agaaacattt gctaatataa caaatagttt ttctatggag ataaatagac
      601 aagtaaatcc aggaacacct agaggtgggg gaaatgtaaa aggtgaagat attaaaaaga
      661 tatctcaaac agatagtata gactcctatg taaaaagaat aaacagtgtt gcagatttag
      721 ttgattatga tattattgaa actcaagaga ctcttgcgaa tcaatcacct gaaagagcaa
      781 agaattttaa aagaacagtt atgttaactg gggttaacga ctcaacaaaa gaaacaaaat
      841 ttgtatcaga agcatataaa ttagtagaag gaaagcattt agaaaatgaa gataaaaata
      901 aaatcttaat gcataaagat ttagctgaaa aaaataacct taaagttgga gataagataa
      961 aaataaaatc taatttattt gatgctgata atgagaaagg tgcagatgaa acagtagaag
     1021 tagaaattaa aggtctattc gatgggcata atagcggtgt agtaagtgca gcacaagaac
     1081 tatacgaaaa tacattaatc actgatattc atacagctgc taaagtttat ggtaataccg
     1141 aagatacagc tgtataccaa gatgcaacat tctttgtaaa aggtgataag aatcttgata
     1201 gtgtaataaa agatcttggg aaattagata taaattggag agaatataat ttgattaaaa
     1261 gttcatcaaa ttaccctgca ttacaacaat ctatatcagg tatttactca atttcaaata
     1321 aattattttt tggctcatta atatttgcag gagttgtagt ttcattatta ttattcttat
     1381 ggatgaatgc tagaaagaaa gaaatagcag tattactatc attaggtata tcaaaattag
     1441 aaatttttgg tcaattccta attgagatgg tatttatatc aatccccgca ttcgttggtt
     1501 cctatttctt agctcaatat acagctgaca agcttgggaa taatatattg aataaggtta
     1561 ctggtgatat agctaaacaa atagctaaac aatctgcatc tagccaatta ggtggtgggg
     1621 cagaagctga aggatttaac aagacattat caagtttaga tatcaatgta ttacctaaat
     1681 tcatgattta tgtagttata tttatgagtt tagtacttct tgtatcattg attatttctt
     1741 cattcaatat attaagaagg aatccaaaag aattattaat tgacaataat taaaattttc
     1801 ggtgcttttt agcaccaaaa atgagagtgt aatataaacc gtgtaagtaa ccatcaatcc
     1861 taggcgggtg gttgcttcac ggtttttatt tttctaaaat aagatcaaga ggtgattaca
     1921 cgatggctag aaagaaaaga aatcctgatg ctgagaagtt agctgaatcc attttgaata
     1981 cttatcaacc cgaatctgta gaggatatgc aagatgtttt aaaagatgtg tttggtccct
     2041 tatttgaaaa aatgcttcaa ggagaattga ataaccattt agggtatgac gcccattcta
     2101 aagagccaaa gaaacatgac aatcgacgaa acggttatgg caataagacg cttaaaacga
     2161 gctttggtaa agtaaacatt gatgttccca gagaccggga ggcttctttt gaacctgagt
     2221 tgattcctaa tagaaaacga gatgtctccg acatc
//
LOCUS       DACEMK010000185         2214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEMK010000185 DACEMK010000000
VERSION     DACEMK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.194"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            461..1615
                     /locus_tag="IW715_002668"
     CDS             461..1615
                     /locus_tag="IW715_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ1023235.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1609..2031
                     /locus_tag="IW715_002669"
     CDS             1609..2031
                     /locus_tag="IW715_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023236.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          847 a          356 c          340 g          671 t
ORIGIN      
        1 caaattatta caacagtcct aacgaagaat tgtttgtaaa acaaacggat aaataataaa
       61 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
      121 acgcgcttat gaagcgctac caaaaattta tcatatttta tgaaaaataa caccccagta
      181 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caactgcaga aaatcaagcc
      241 ttaaaaagag cttttgacct agaccaatga aaaatcacaa agtgagcccc tgtatccctt
      301 ggtagagagg ctacttggca tagtcgacat tatgttaact aacgctataa ataacgatta
      361 tgttcccaaa acaatatttt atgaactata acaaaaattt gttatcgcat attgtatatt
      421 ttaattatag gtgtatacca cttaaattta acacaaaggc gtgattattt tgaaacccaa
      481 aaagaaaatt tatctaggtc tcctcttttc cttgatgatt ggaattttta gtataggaat
      541 tacaaatacc tatgcttcag aaaattctaa tttcaatatc aactcccaac caattgaaga
      601 acaagcaatt aattttgcga aaactgattt tgaacactcc ataacttcaa ttcttttaat
      661 ggaaaataac accgaaactg atattaacta tactcttggt tcgccattta aaatgctcga
      721 tggagaaaat acggcaaata gttctatttt ctttccagta ttttcagacg ctgataatac
      781 gattaaatat atttataccg tcaaaaaaaa tacaaatgga gaatattcag gaagtatttc
      841 acaattttta gcaaaagaac ttcaaaatct agtagatagt aatataacag atacaacctt
      901 attttttcaa gataatagca tttattattc cgataatgga caaattaaac tactttggaa
      961 atcaccttta agtacagaaa aaaatggtgt ttcttccttt acagaaaaaa atgatcttat
     1021 atccgtaaat ataaaaaata aaattaaaaa tttctctaaa aaacaaaatt ttcctgcggt
     1081 taaacgtgta aaaagtgtct cctccggaga taactttatt atgattaatt ggcgtatagc
     1141 agaaacccaa acaagttttc cgtggtgtgc tgcttacgta gaagctgcta tcttatgtaa
     1201 caaaacagat gtacatgcta cttcagctag agccattatg cattatacct atcctaattt
     1261 aaacaacagt caaattgaat caaaaagtct taatcaagat caaataataa aatatgcaaa
     1321 ttactgtggt gtttatccta aaaaagtagc acgaatacta aacctttcag aggtccaaac
     1381 acaactaaga aaaggaaatg ccatttatat gggaactgct ggttatggaa attatagtaa
     1441 cagccgtcat gcacttgcat tatttgggtg ggtcaatcaa ggtggttatc aaacttacta
     1501 tgtgtggaat ccttggtata actacccaac tgtaataagt gcaaatgcag gaccagttac
     1561 aattcctgta cctggaggtg gttttacttg ggacaatacc attacaaatt ggtaaataaa
     1621 cccattgctt tagttatggg tctagtgtta gttcttttct taactagttg ttcaaaaaaa
     1681 caagaaatac aaaagactga tctgattcct tttctaggaa cttggcaaac taaagatcat
     1741 tcagaacgtc tagtaatcac taatttaaat acttcaaaaa atatagaaca atataccatc
     1801 ttagtaaaag agaacaatca taaaattcaa atcattgata aaaatttaaa tataaatgaa
     1861 caagttttca tttacaaagc aacaaataat ttactaacac tgcgctctaa taaaaaacaa
     1921 ttagattaca cttttagttt ggattctcca aaaaagctcg tatttatttt gacaacgaat
     1981 gatgggacag aagggacagc taaaccaatt gaatttttta aagctgattg atgaatagtt
     2041 gtttgatatt acaaaacaat aatttcaccg aaggtgaaac aagcgaaact ttacaaaaag
     2101 tggctagcat ttgctagcct tttttgtatt gtggagcgtg ggttttgagg gggattcaag
     2161 ggggagcttg cgcccctttg aaaagcggtt atttgcacca aatacgtagg tatt
//
LOCUS       DACEMK010000186         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEMK010000186 DACEMK010000000
VERSION     DACEMK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.195"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW715_002670"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IW715_002670"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1023237.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IW715_002671"
     CDS             complement(1431..1823)
                     /locus_tag="IW715_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1023238.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          451 c          342 g          663 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACEMK010000187         2030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEMK010000187 DACEMK010000000
VERSION     DACEMK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.196"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            26..412
                     /locus_tag="IW715_002672"
     CDS             26..412
                     /locus_tag="IW715_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1023239.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            716..2008
                     /locus_tag="IW715_002673"
     CDS             716..2008
                     /locus_tag="IW715_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1023240.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          687 a          385 c          383 g          575 t
ORIGIN      
        1 ataaaaataa aaaaggagat ctttcatgaa aaaaatttta gggttgatcg ttatcatatt
       61 gattgctttt ataggatgga aaggttggga ctactatcag tctacttatg taggtaaaga
      121 ttattatgcg gtagtcaaag ctccaatgcc tgcagaaact gatatcaaag cagataatgg
      181 ggaagtaatt ggaagaggtt ttaaatacaa cgtagatgct tatgctgaaa acggtgaaaa
      241 aagacagtta gatttcgatg tcattacttc tggtgactct gccaatggtt ctgcttaccc
      301 cgaaggaacg atcttgcagt tgaaagcaag cgaaaagcgg atcatagaga aaaaagtaat
      361 taccagcgat aaggtaccct catctgtaaa agaaaaatta ggcctatctt aagctaagaa
      421 tatatcgcaa aaaagcactc gaagtgagtg cttttttgct tactgattct agaaaacaaa
      481 aaaacagaat taaccttcga agtcagcctc actgttctta aagtcctaaa acaaactaaa
      541 gagcaaggca atcaatcgat ttctatacaa acaagggact gtgacaaacg gctctatact
      601 ttttgatgtt ggtggatgac accagcatct tttttatttt ccaaaacata tagaaaagac
      661 accttgaaat cctttagaat taatttggcg aaccaaccca aaaggaggaa tcaagatgtc
      721 ttacactcac cttatcaaag aaacattaga tattctagac ttatcagtta cttttaacga
      781 aaattgtctc acaaaagaaa aatacaaagg gcaaatctgt catatttatc gtggaaactt
      841 gatttataca gcccaagaat gcatccactg taaacaccag attgcttcag atattgtgcg
      901 ttggggtaca acaaccgtcc ggttgttaat gaacgatgtt tctgaatacc gtacctacct
      961 cgaattgaaa aagcaacgct tcaaatgcaa agcttgtcag cggacctttg tcgcagatac
     1021 ttctgttgcg aaaaaacatt gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg
     1081 tctgaaaaaa aatacctcaa tgacagagat cgccgctcag aaaaatctat cggtctcttc
     1141 tgtttaccgt attatgaagc gattttatcg tccgttgaat ccttttcgga caccgttacc
     1201 taaggtactt tgttttgatg aattcaaatc agttcggggt gtttcaggtg ctatgagttt
     1261 catcatgatg gatggacaaa cacagcgctt attggacatt gtcgagaacc gacaactgcc
     1321 ttttttaaaa cgttatttta gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat
     1381 cgtgattgat atgtacgctc cttatgtatc gctcgttaaa aagctgtttc caaaagcaca
     1441 gctgattatt gaccggtttc acatcgtcca acatattggt cgtacctttc gcaatcatcg
     1501 aatcaaagaa acgaaccaac tattaaaaag caaagaacaa aaacactatc aattaggtaa
     1561 gcaattaaaa agatactgga aacttcttca aaaagatgaa cgcaagcttg attatactcg
     1621 acgcctttgg cgacctggct tcaaagctca tttaacagag accgatattg tggatcgctt
     1681 actgaaaggg agccctgctt tgcgtgtagg ctatcagtta tatcaagact ttctttacgc
     1741 tgtaaaggaa cgagactacg tatcgtttga agaactactt accaataata tcatgcttcc
     1801 cgaaggctat cagacgacct tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc
     1861 tttacaacaa agttattcaa atggcccact tgaatgtctg aacaaccata tcaaagtgtt
     1921 aaaacgaaat gcatatggct ttcgtagctt ttataatttc aaattgcgca tcatgattcg
     1981 tcatggcaac gcattgattt tcaactaaaa aagatcaaga ggaactgatc
//
LOCUS       DACEMK010000188         1979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEMK010000188 DACEMK010000000
VERSION     DACEMK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.197"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(325..528)
                     /locus_tag="IW715_002674"
     CDS             complement(325..528)
                     /locus_tag="IW715_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023241.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="IW715_002675"
     CDS             complement(728..1744)
                     /locus_tag="IW715_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1023242.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          557 a          459 c          309 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DACEMK010000189         1878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEMK010000189 DACEMK010000000
VERSION     DACEMK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.198"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            294..1778
                     /gene="guaB"
                     /locus_tag="IW715_002676"
     CDS             294..1778
                     /gene="guaB"
                     /locus_tag="IW715_002676"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1023243.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          643 a          335 c          424 g          476 t
ORIGIN      
        1 tactcgaaac taagcagctt tttcacagcc tctccactaa acggtgttca aaagtttgca
       61 tctgcggtaa taaacccaac caattcaaaa acatgagaag ctgtttttga ttggctgttc
      121 ttattacttg atgcaggaca gcttttttac aatctctttt aatacacaca gatgtaaacg
      181 ctttatattg gtattctatg agaaagctaa cttgattcca agtagatcat tgacttaaat
      241 gaaaaaatca attattttaa tagataaact aaattagagg agtggtacta aaaatgtcca
      301 actgggaaac aaagttcacg aaaaaaggat ttacttttga tgatgtctta ttgataccag
      361 cagaaagtca cgtgttacca aacgaagtgg atatgagcgt acaattagct aaaaatatta
      421 agttgaatat tccaatcatc agtgcaagta tggatactgt taccgacagc aagatggcca
      481 ttgctatggc aagacaaggc ggactaggtg tgatccataa aaacatgacg atcagccagc
      541 aagcggatga agtaagaaaa gtcaaacgtt ctgaaagtgg cgtaatcatt gatccgttct
      601 tcttaacgcc acagcatctt gtagcagacg cagaagaatt gatgagcaaa tatcgtatca
      661 gtggtgtgcc aatcgttgaa acacttgaaa acagaaaatt ggtagggata attacaaacc
      721 gcgacatgcg ttttgtaaca gattatcaca tgccgattgc tgatgtgatg acaaaagaca
      781 acttagtgac tgccccagta ggtacctctc taaaagatgc agaaaagatt ttgcaaaaac
      841 ataaaatcga aaaattacca atcgtagata acgaaggtcg tttaagcgga ttgatcacga
      901 tcaaagacat cgagaaagtg atcgaatttc caaatgcagc aaaagatgaa catggtcgat
      961 tgcttgtagc agcagccgtt ggtgtaacaa gcgatacatt tgagcgtgcg aatgctttat
     1021 tagaagcagg ggcagatgca atcatcatcg atacagctca tggacatagt gcaggtgtga
     1081 tccgaaaaat tcaagaaatc cgttcaactt ttgcagatgc aacattgatt gcaggaaacg
     1141 ttgcgacggc tgaagcgaca aaagctttat atgatgcagg tgtagatgtc gtaaaagttg
     1201 gtatcggacc aggatcgatt tgtacgacac gtgtggtagc cggtgtaggt gtacctcagc
     1261 tcacagctat ttatgatgca gcatcagttg ctcgtcaata tggcaaagcg attattgcag
     1321 acggagggat caaatattct ggtgacatcg tcaaagcatt agctgccggc ggacatgcag
     1381 tgatgctagg aagtatgttg gcagggacag atgaatcacc aggtgaattc gaaatcttcc
     1441 aaggacgtcg attcaaaaca taccgaggta tgggctcatt aggcgcgatg gaaaaaggtt
     1501 caaaagaccg ttacttccaa ggtagtgtca atgaagcaaa taaattagta ccagaaggaa
     1561 tcgaaggtcg ggttgcttat aaaggaagtg ttgcagatat tattttccaa atgatcggcg
     1621 gtttgcgttc aggaatgggt tatgttgggg cagccaatct acagcaatta cgtgatgaag
     1681 cgcaatttat ccaaatgagc ggcagtggat taaaagaatc acaccctcat gatgtacaaa
     1741 tcacaaaaga agcaccaaac tattcagttg aatcttaaaa acattaaaga gcgtgggaca
     1801 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agtagaagca
     1861 actccttcgg aaataaga
//
LOCUS       DACEMK010000190         1861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEMK010000190 DACEMK010000000
VERSION     DACEMK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.199"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            513..1127
                     /locus_tag="IW715_002677"
     CDS             513..1127
                     /locus_tag="IW715_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1023244.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1151..1501)
                     /locus_tag="IW715_002678"
     CDS             complement(1151..1501)
                     /locus_tag="IW715_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1023245.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1495..1758)
                     /locus_tag="IW715_002679"
     CDS             complement(1495..1758)
                     /locus_tag="IW715_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1023246.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          618 a          338 c          276 g          629 t
ORIGIN      
        1 gtaaaagctt ttcatatcga ttaaaaagta atcatttact ggctcttttg aataatccat
       61 cataaataac actccattct ttcacattat acaaacatat gttcgatatt gtaaagaaga
      121 aatagctaaa tacataaaaa aagaagcata ggtatgctat caccctgctt cttcttttga
      181 tttgtatgat caatttattc tatcacttta ctaattaatt tgaaaatcta ataaaaactc
      241 tcactctaaa agagtgagag actgcaaatt agagtacgga cttttttgga gttccgttct
      301 ttaatattaa caaatgaata aaatccttac aacatttgag aataagttat cagtagacac
      361 ttacaaaaaa ttctcatact atttcctcca tcattagttt ttgtctatat aaggtatacc
      421 ctaactagac acgaccgcga cattgaaaaa tctggttttc tcgtgtatga ttagggtgtt
      481 ttttaaatag ttgcagaaag taggtaaaaa ttttgagagt tgcatacata cgtgtctcat
      541 ccatcgatca aaatgaacaa cggcagattg aagaaatgaa aaaattcggt gcagagagaa
      601 ttttcattga gaaacaatct ggcgcaacga ttactcatcg acctgttttt caagaagcac
      661 tggatttcgt ccgagatcaa gatattttta ttgtggaagc tattgatcga cttggaagaa
      721 attacgacga gatcatcgct tcagtcaatt acttgaagaa gaaaaatgtg cagctgatca
      781 tcacaagtct accgatcatg gccgaagcaa ttggcaatcc tctcctggat aaattcatta
      841 aagatttgat cattcaaatt ttggccatga tcgctgaaca agaacgtacc gaatctaaac
      901 gacgtcaggc acaaggcatt aagattgcaa aagcgaacgg tgtttataaa ggacgtccta
      961 agctatacag tgccaatgca aaagatcccc aacgtcgtct tgtttataaa aacatcgttg
     1021 aggatctaaa aaaaggtgtt gcgatcgcta agattgcaaa agactacaat gttacgagac
     1081 aaaccgtata tagaataaaa aaagacagca tggtcaatca cgaatagttg atcatgctgc
     1141 tgtttctatt ttatttgctt actttttgaa aaactgttct aactttcatc aataactcct
     1201 caactaaact atcatgcgct ttttccaaaa aaatacactc tctggcttca taatcaattg
     1261 tcgttagttg atctaataat acttttcctg ttgtcttcat ttcatattct gagtttatat
     1321 ttacatatag cggatagttt ctagttgtac tagaaattgg agcaactgta acaacgttac
     1381 tataatccgc aataatatca tgacttaaaa caatataagg tcttttacct gtctgttcgt
     1441 gtccagatcg aggtgctgta ttcaataata gaatatcgcc ttgtctaggt tttctcacca
     1501 tttttcatta cctactgctt cacctaaatt ttgaatcgtc acatgatttc tttcatcagt
     1561 gtaattttca aataactgtt caattgttaa ttcattttct atattagccg cttcgataat
     1621 tagccgcttc tgaccgttta cttcttcttg tattatcttc actttgtctt ctgtatgaat
     1681 tccaagatat tccataatat actttggcaa tctaaggcca ttactatttc cccatttttt
     1741 tataggtaac atttccatag tagacacctc tttctgtata tacatagtat aacatacata
     1801 agaaaaaacg tatatactta attatccttt tctattaaat tagattaaca ttccatgaat
     1861 c
//
LOCUS       DACEMK010000191         1859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEMK010000191 DACEMK010000000
VERSION     DACEMK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.200"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(315..1607)
                     /locus_tag="IW715_002680"
     CDS             complement(315..1607)
                     /locus_tag="IW715_002680"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1023247.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          536 a          388 c          331 g          604 t
ORIGIN      
        1 tggagaggtt gtgaaaaagc tgctctgcat caagcagtaa gaacaatcag ataaaaatag
       61 cactccatat ttttcatctg attgggctta gtgccgtaga tgcaagcttt tgaacaccgt
      121 ttgttcggaa aaagggactg tgacacggtt tttgtcacag tcccttattc tgaataaaca
      181 gtgttcaaga gacagcctat cggcaataag gctaaacgtc tctttcacta cctcttcaag
      241 ttattattat cgtatagata aacacttcga ctcgctctgg taagaacgtc ctgattcagt
      301 cgtcgttttt atccttagat ttgcgcccag acgctttcta agatgtttgt ttgtgttcta
      361 tcaggtccga cagaaaatgt tgagatacgt acaccaacaa gctctgagat acgacgtaca
      421 tagttacgtg cgttttctgg cagttctgca agtgttttgc atcctgtaat gtcttctgac
      481 cagcctggca attcttcata gattggtttg cagcggttca actctttcaa gcttgctgga
      541 tagtggtaga ttagttcgcc atctaattca taagccgtac aaatttttac tgtttccaat
      601 ccacttaata catcgatcga gtttaaagat aggttggtaa tacctgaaac tcgtttagaa
      661 tggcgcataa ctacagaatc aaaccagcct acacgacgcg gacgaccagt tgtcgtaccg
      721 tattctcgtc ctacttcgcg gatttgttct cctgtttcat caaataattc agttggaaat
      781 ggtccatcac cgacacgtga cgtataggct ttgcagacgc cgacaacttt attgattttt
      841 gaaggcccta caccgcttcc gattgttacc ccaccggcta ctgggttaga agaagtaaca
      901 aaaggataag ttccctggtc gatatccagc attacacctt gtgcgccttc aaacaacacg
      961 cgtttgcctg catccaaagc atcattcaag ataacggaag tatctgtgac gtattgtttg
     1021 atttgttgtc cgtattcata atattcttca aaaatatcgt caaaatcaag tggttctgaa
     1081 tcaaacattt ttacaaattg acggtttttg tcatctagat tcaaacgaag acgttcttca
     1141 aaaatttctt tatctaataa gtctgcaata cggataccta cacgagctgc tttatccata
     1201 taggcaggtc cgattccttt aatcgttgta ccaatcttat tttcgccctt agcatcttct
     1261 tgtaattggt ccaatttgat atgataaggc aagattacgt gcgcgcggtc agaaatacgc
     1321 aaattacttg tttctacacc tttttcatgt aaataagcta attctttaac caatgattta
     1381 ggattgacta caacgccgtt tccgatcaca ctgatcttgt ctttataaaa aatcccagaa
     1441 ggaatcaaat gcagtttata agtcacaccg ttaaacttaa ttgtgtgccc tgcattgtcg
     1501 ccaccttggt accgtgcaat cacttcagca ttttcactga gaaagtctgt gatttttcct
     1561 ttcccttcat cgccccattg cgtacctact actactacag atgacatgtg aacacctcgt
     1621 tctatattat ttaatcctga accattgtat caataataga tacatatttc aaataaaatc
     1681 gaatgatttt tatttttatt ttataaaaac gcttaaaaaa cgaactttat ctttttagaa
     1741 tggttattaa aaaagaggtt gtaacagaag tggtcagctt caagaaataa gaaggaatgt
     1801 acgaaaattg cttctcaaat ttttgtgaaa tttcgactta tttttcgaag aagctactt
//
LOCUS       DACEMK010000192         1806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEMK010000192 DACEMK010000000
VERSION     DACEMK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.201"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(99..1118)
                     /locus_tag="IW715_002681"
     CDS             complement(99..1118)
                     /locus_tag="IW715_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1023248.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1129..1335)
                     /locus_tag="IW715_002682"
     CDS             complement(1129..1335)
                     /locus_tag="IW715_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1023249.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(1468..>1806)
                     /locus_tag="IW715_002683"
     CDS             complement(1468..>1806)
                     /locus_tag="IW715_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1023250.1"
                     /translation="WFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPS
                     QRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSY
                     VSIDDLSNLI"
BASE COUNT          521 a          366 c          299 g          620 t
ORIGIN      
        1 gggtgatagt gaagatatgc gataacatca ctattttatc gaatgatttt tatagttgtc
       61 aatatagtta tgtaatgctc ctcaacgagg agctattttt atcgtttagg aatatttaaa
      121 taccaacgtt tgtcatggaa atcttgcgca ccgcctttag tgtttccctc tggatcattc
      181 gttgcccgca tcatgacata gactttctta ttagggaagt tacgcatgtt aaaagataca
      241 tgatagccaa catttccaga agtattataa gcttgattta catctgatct ataaattcca
      301 tcagctctta ctcgagctaa ttctttccca gtattgtaat ccataatgaa gatatactcg
      361 tatttatagt tagcaatgtg ccatccagct acatgcaaat ttgcattttc gatttctcca
      421 aactgatcaa tgtgggcgta attcgttcca tctgtcagtg taggattagc tgcaccagct
      481 cgtgttggat caatgacagg cttgttttca gaagttgttg gattttcatc ggtaaatcca
      541 tgagctaaat catatgctaa tttttcttta cttacgccca tttcagaaag ataaccgtaa
      601 ggatctgtat gatcgcccca gatgttttgt gttacccata aatgcgattt gattcccggt
      661 tggttataag gagtgtccaa cgttaatgga ataccatatt tcattgctga atctctagcc
      721 aattcaacgt atgccttgta gtttttctca aacgttgctt tatcatgtgt gtgttgtaac
      781 tcaatctgca caggactgtt ggcattagca tacgaaccag caccgtactg tacataacca
      841 ggttgaccga cttgataaac aattccgccg tctcccacaa tataagcagt ataagcgcta
      901 gtccatgaac gttgcatata ctgcgcttca ttgcgtcctg ttgctgtttc attagccgtt
      961 tcatgcagta aaatatactg attatttgct acttgtgagc taccttcatt tgcgcccaaa
     1021 ttaaattcat tgttgatagt ataggcaaat ccgttaattg gcaataaaaa aagagccgtt
     1081 aacaggctca tcgcagtaat agtaattttc tttttcatat ctatcctcct atttatctga
     1141 tgaaattttc ttatacgttc gatttgatac acctaaaata ctccctagaa atgctccaaa
     1201 acctgtaata atcatcaccg ttatatctgt gtaccgccag ttaacacctt ttccgacaag
     1261 accgacaaaa gtcgctaaag cggggataac tactatagcg atccatttca aaatatcaaa
     1321 ggttctattg ttcatactat ccacctccct tcattggttg ggctactttc tttttcaatc
     1381 gcttgttttc ttctatcaat atttctcttt ctgaaagcaa ggcatcaatt tgcccgtcca
     1441 gttgttcaat tttatctttc ttaattgtca aattaagtta gacagatcat caatacttac
     1501 gtaactcaaa aatacttcca aaggtgttcg ataatccagt gattttctag gaatattatt
     1561 tcgtttagat gcgacagatt gaataaaaaa ttcatctact gaattgaaat ccatagattt
     1621 caataaacca tctctacgta acaatccgtt agaattctcg tttaggcctc tttgagacgg
     1681 cgttcctgga tcagcgaaat aaatggcaat atcattgaca ttactgatct gtttccaatt
     1741 tgaaaattcc tttccacaat caaaagtgat ggatttgaat aggtttttcg gtacagattc
     1801 aaacca
//
LOCUS       DACEMK010000193         1713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEMK010000193 DACEMK010000000
VERSION     DACEMK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.202"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..1391)
                     /locus_tag="IW715_002684"
     CDS             complement(<1..1391)
                     /locus_tag="IW715_002684"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1023251.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIV"
BASE COUNT          478 a          327 c          317 g          591 t
ORIGIN      
        1 tccacaattt catcaaattc tgcgacataa gcatacttgt ctttaattgt acgggtttta
       61 taggcctcta aaatagcgtc aatatggtaa tctcgtagcg tattttgcgt ttttattttt
      121 tcaaaatctt gtgacgcatc aataaagagc acatcttttt tcgcacggtc tttcttcaag
      181 ataattacgg tcgttggaat ggacgtatta aagaagatgt tagcaggtaa gccaatgacg
      241 gtgtcaatgg caccattttc taacaaggct ttgcgtatct tcccttctgc gccccctcta
      301 aagagcaccc catgtggcaa aacaatggcc atgacgcccg tatctttcaa atgatagtag
      361 ccatgtaata agaaagcaaa gtccgctttt gatttagggg ctaatacgcc ataggcagag
      421 aagcgtgggt catctaagaa gcctttatca gccgaccatt ttgcagaata cggtgggttc
      481 atgagcaccc catcgaagtt cgttggctct tctgttggcc agtcttgatc caaggtatcc
      541 gcattatgca agtgttggtt ggcaatggga accccatgca aaatcatgtt catacgcgcc
      601 aaattatagg tagacgtatt caactcttgt ccgaaataag aaatggttcc cggttgatgg
      661 gaatattttt tagcgttcaa aagtaacgag cccgaaccca tggtcgcgtc gtaaacagaa
      721 aagccttttt tatcttcttt tccttgtaaa acaatttgtg tcatcaattt tgcgaccggt
      781 tgtggggtat agaattcacc ggctttttta ccggattcag atgcgaattg gccaattaag
      841 tactcatagg catcgcctaa gacatcgcca gcatgccccg ccatgtttaa cccttctaat
      901 tctttcataa catctgcgat ggttttattt tgtttttgtg gggttgaccc cagtttttta
      961 gaatacagat caatgtcttc aaataagttc tcaaagtttt cgctggattg ttcaatgtca
     1021 cggaaacctt gtaccaggtc ttctaattga aaagatccat catgaatggc tgccacttgc
     1081 gcggtaaagg tcaatgcggg tgcgatggta tatgaaaact catcttttag ttcatacaag
     1141 agatcatcct ttatatctgc atctttaaat gcttcaacgt aaaggtcttg tgctttattt
     1201 aggtcgtccg tttcttcctc caatagaacg gagacatacc gcagcatgtt gtcggataag
     1261 tatttataaa agaccaagcc tagtaaatag ctcttgtatt cattagcgtc catttttgaa
     1321 cgcaggatat ctgcggaatt ccacagtgcc tgatataagc tttttgaatt tttttcgatt
     1381 tgttctgtca ttggttattt tcctcattcg tgttgtattt tttataaatc agttttttaa
     1441 aaaattagtt actatttgaa cttgtttttt caccatatgt attttaatga aatggataag
     1501 ctacatttgc ttagacaatt catgtcttac ttaattctta ttataacaaa cctatttcac
     1561 ctttttcgat acattaaata ataagtaact taaaataagg ttcgaataac cttgttaaat
     1621 agtggttttc ttatcttata gtatagaaat caagagatta cttccgatat aacatttttt
     1681 taaaaaaatt agattgtaat gttttttata ttc
//
LOCUS       DACEMK010000194         1701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEMK010000194 DACEMK010000000
VERSION     DACEMK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.203"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            466..1065
                     /locus_tag="IW715_002685"
     CDS             466..1065
                     /locus_tag="IW715_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1023252.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1129..>1701
                     /locus_tag="IW715_002686"
     CDS             1129..>1701
                     /locus_tag="IW715_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023253.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          612 a          257 c          321 g          511 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
       61 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
      121 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
      181 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
      241 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
      301 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
      361 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
      421 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
      481 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
      541 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
      601 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
      661 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
      721 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
      781 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
      841 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
      901 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
      961 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     1021 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     1081 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     1141 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     1201 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     1261 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     1321 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     1381 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     1441 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     1501 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     1561 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     1621 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     1681 tttgacaact ttataggaga t
//
LOCUS       DACEMK010000195         1674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEMK010000195 DACEMK010000000
VERSION     DACEMK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.204"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            190..546
                     /locus_tag="IW715_002687"
     CDS             190..546
                     /locus_tag="IW715_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1023254.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            556..1455
                     /locus_tag="IW715_002688"
     CDS             556..1455
                     /locus_tag="IW715_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1023255.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          560 a          292 c          344 g          478 t
ORIGIN      
        1 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
       61 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
      121 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
      181 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
      241 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
      301 caagtcagtt gccaattgga cacggcattt cgattgatca ttgtcaaaaa agatacacaa
      361 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
      421 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
      481 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
      541 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaag
      601 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
      661 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
      721 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
      781 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
      841 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
      901 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
      961 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     1021 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     1081 cctaaacggg catttatcat gtatcaatct gctccttatg agcgctggaa caaattaaat
     1141 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     1201 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     1261 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     1321 gaacagctgt tagatagcta tgaacccatg aaattacgtg tcctcttgcg tgcccatcat
     1381 ccggcctttc aagcctatca aacacaacat ttgcaagagg aatttgaaca ggcgatgaca
     1441 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     1501 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gtggaaaata
     1561 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     1621 ttattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttat
//
LOCUS       DACEMK010000196         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEMK010000196 DACEMK010000000
VERSION     DACEMK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.205"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            131..505
                     /locus_tag="IW715_002689"
     CDS             131..505
                     /locus_tag="IW715_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1023256.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IW715_002690"
     CDS             complement(619..1248)
                     /locus_tag="IW715_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1023257.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IW715_002691"
     tRNA            complement(1382..1454)
                     /locus_tag="IW715_002691"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACEMK010000197         1609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEMK010000197 DACEMK010000000
VERSION     DACEMK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.207"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(209..1396)
                     /locus_tag="IW715_002692"
     CDS             complement(209..1396)
                     /locus_tag="IW715_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1023258.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          479 a          334 c          282 g          514 t
ORIGIN      
        1 aaacagatcg gagtaatggt tatgcttttt aatgagacag tcaagtccac aatcttgtgt
       61 aaaagttcaa cctcatcaaa agcacaatgt tttgcgttgt gaacctaact gccatgcctt
      121 tctttctgag gagagaggtc aggagcagta gcgcagcgac tcttgatgtc tctcctcaga
      181 aagaagagac tcgcacaggt tcatggtgct aaatgttatt cgaattaaat tctaaatatt
      241 ttcgtggaga agccagccac tcagtgtctt tttccatcaa aagtgttcca attatccgag
      301 tgatggaatt gatatttgga aaaatccgaa tcactcgttc tctccggcga atctcttcgt
      361 tcaaccgttc caacacattg gttgtacgaa tccttcggcg gatattttca ggaaaagaca
      421 aaatttgaat agcatcttca aagccgtttt ctaaaatgtc gcaagcagca cttaattttg
      481 aatcacaacc atatttttcc aagaagtgct cttttctttc acgtgtcaat tcaagttcag
      541 aggctttaaa gatactcttc aactcgtctt ttacatcaga agaaactttc tttggcagct
      601 tatcaaaaat atttctcaaa aagtgggctt ggcatctttg ccagctcgca cctaaaaagt
      661 tcttgcgaac agccttaact aaccctttgt gtgcatcaga aatgaccagt tttacaccga
      721 acaaacctcg ttgaacgaac gattgaaaaa cggtatccca attatcttcc gattcgccgt
      781 cttgaacatc aaaaccaaga atcgtacgtc gtccatcaga atcaatgccg ataccaatat
      841 atacaccttt tgagacaata cgatgatttt cccttacttt catatacagg gcatcacaca
      901 tgaaaaatgg atattccgag ccttctaggc tccggtttct ccactcaaaa acaaatggat
      961 ccaactgttt catcagattt gaaacgaatg atttagaaac agtcgcacca tccgttagca
     1021 gttcaactgt tttagttact ttgcgagtgg agacaccaga aatgaccatt tcaatcatgg
     1081 cagcgatcaa agctttttcg cttcgctgat atctttcaaa gatagtagga ctaaacttac
     1141 cgtctctggt tcgtggaaca tgaagttcta acgtaccaat tcgagttgta aacgaacgat
     1201 cgtagtaacc atttctttgt gattgccgtt cttctgaacg ttcataggca tttgcctgaa
     1261 tatattgatt tcgttgttct tccatcagct gattaaacat gatagtcata agtgtttttg
     1321 cggtatcatt cgcaccgctt tcgtttataa ttgcttgaat ttcttcgctt tcaaaagtaa
     1381 aatgtacttg agtcattgat atcagtccta ttcgagtatg ttttctgcaa aaaacattgt
     1441 actcctaact gagatcaatg gcttttttga tgcttcactt ttacacaatt atatggacgt
     1501 aatcaatgag acacaattat ggtttaaatt tgacccttcg aatagattta tcaaagattt
     1561 ttataaagta tggaattcag aagttttctt tttagcaatc gaagatagc
//
LOCUS       DACEMK010000198         1580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEMK010000198 DACEMK010000000
VERSION     DACEMK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.208"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1297
                     /locus_tag="IW715_002693"
     CDS             128..1297
                     /locus_tag="IW715_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1023259.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          567 a          254 c          258 g          501 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataata
//
LOCUS       DACEMK010000199         1568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEMK010000199 DACEMK010000000
VERSION     DACEMK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.209"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1307
                     /locus_tag="IW715_002694"
                     /note="aph(2'')-Ia"
     CDS             <1..1307
                     /locus_tag="IW715_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ1023260.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1437..>1568)
                     /locus_tag="IW715_002695"
     CDS             complement(1437..>1568)
                     /locus_tag="IW715_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1023261.1"
                     /translation="RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          648 a          135 c          267 g          518 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      601 attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata
      661 ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag
      721 aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt
      781 ttttaagaca aatgcacggt ttagattata cagatattag tgaatgtact attgataata
      841 aacaaaatgt attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg
      901 atatagaaaa agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg
      961 agggtaaaaa gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca
     1021 ataatagatt aactggaata attgattttg gagattctgg aattatagat gaatattgtg
     1081 attttatata cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata
     1141 tattaagaat gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg
     1201 aagaatatta tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta
     1261 tcgaaaatgg tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat
     1321 gaaaagctat tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag
     1381 gaagtgagtc aagtccagac tcctgtgtaa aatgctatac aatgttttta ccatttctac
     1441 ttatcaaaat tgatgtattt tcttgaagaa taaatccatt catcatgtag gtccataaga
     1501 acggctccaa ttaagcgatt ggctgatgtt tgattgggga agatgcgaat aatcttttct
     1561 cttctgcg
//
LOCUS       DACEMK010000200         1515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEMK010000200 DACEMK010000000
VERSION     DACEMK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.210"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(249..773)
                     /locus_tag="IW715_002696"
     CDS             complement(249..773)
                     /locus_tag="IW715_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023262.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
     gene            complement(798..>1515)
                     /locus_tag="IW715_002697"
     CDS             complement(798..>1515)
                     /locus_tag="IW715_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAQ1023263.1"
                     /translation="TSQRATYRAVCSVLFPNGDFASRSRFTRLSSNLAYTIKIIRYFF
                     IKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVT
                     KTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVP
                     PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYS
                     VLLSRAQRRFEGTLRYSLGY"
BASE COUNT          481 a          239 c          222 g          573 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaacctacc
      781 cgtggtgcta gttaatttta atatcctaaa gaatatctca aagttccttc aaaacgtcgt
      841 tgtgctcgac ttaatagaac actgtaagcc agtaatatgc tttcaaatct actctctagc
      901 cctttaacag aacgtgagtt gaaattttga caacctaatt tttctaagga agaaaaaaca
      961 gtctcaactg tttttctttg ttttccaagt aagctgtggt ctagtttttc tgatttatcc
     1021 atgtttttac gtggcggaac ggatatagta atgcccttaa gggcaagctt ttcatggatt
     1081 ttatggctaa tatatccttt gtcccctagg atattgggaa gattcgcttc ttccgataaa
     1141 gtctctaaca ctttcacgtc atggacacct ggattcgtga ttgagtaagt aatcggaaag
     1201 ccagtcttag taacgatcat atgaatttta agaccataaa aataagattt ttttgttgag
     1261 ttatagccaa ctttggctat ttgattcaat agttttgctt gtctatttct aactggttta
     1321 cataatggac taggaaaact atctataatt ccgactagtt cacctttggt gagcttcttg
     1381 atgaaaaagt atcgaataat cttgatagtg taagctaagt ttgaacttaa gcgagtgaat
     1441 cgactcctac tagcaaagtc accattagga aataagacgg aacaaaccgc tctatacgtg
     1501 gctctttggc tagta
//
LOCUS       DACEMK010000201         1515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEMK010000201 DACEMK010000000
VERSION     DACEMK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.211"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            287..694
                     /locus_tag="IW715_002698"
     CDS             287..694
                     /locus_tag="IW715_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023264.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IW715_002699"
     CDS             708..1109
                     /locus_tag="IW715_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023265.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IW715_002700"
     CDS             1111..1482
                     /locus_tag="IW715_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023266.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DACEMK010000202         1445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEMK010000202 DACEMK010000000
VERSION     DACEMK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.212"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..226
                     /locus_tag="IW715_002701"
     CDS             <1..226
                     /locus_tag="IW715_002701"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1023267.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IW715_002702"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IW715_002702"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IW715_002703"
     CDS             533..1336
                     /locus_tag="IW715_002703"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ1023268.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DACEMK010000203         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEMK010000203 DACEMK010000000
VERSION     DACEMK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.213"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(203..1186)
                     /locus_tag="IW715_002704"
     CDS             complement(203..1186)
                     /locus_tag="IW715_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1023269.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          390 a          298 c          266 g          439 t
ORIGIN      
        1 tgttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca acctctgata
       61 taaacggtgt tcaatcaact gaccctcggt attagctcaa aaaacgacaa aatatgaaga
      121 gctatttttg tttggctgtt cttattactt gatacaggac agctttttca cgacctctaa
      181 ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag cgttggatgt tctttttcaa
      241 aggtctctaa gatctctcgt acttcatcag tagatttcgt attcatcaac tgaactcgca
      301 agtcatttgc tcctggaaat cctttgatat agattttaaa gaaacgatgt agtcctgtga
      361 tggaacgcgg gatgatttct gaataatgat cttgcagatc tagttgcaga cgaagtagtc
      421 cgatcagttc tttttctgta tgttccctcg ggtctttttc aaatgcataa ggattcttga
      481 aaatccctcg gccaatcatg atgccatcaa cgccatattt ctcggccaat tcttctcctg
      541 tctggcgatc ggggatatct ccattgatcg tgattagtgt ttgtggagcg atttggtcac
      601 gtaaagcaat gattttagga atgacttccc aatgtgcatc tactttactc atctctttac
      661 gcgtacgcaa atggacagaa agattggcta tatcttgatg aagtaggtga ctgatccatt
      721 cttccatctc agacatttcc gtatagccga tacgcgtctt gacgctgaca ggcaaaccgc
      781 cagctttagc tgcttcaatc aattgtgccg ctacttctgg acgaaggatc aagccactgc
      841 cttttccacg ttctgctaca ttagggactg ggcagcccat gttgatatcg attccttgga
      901 agcccatttc tgccatagca atgctcattt ctctaaaaaa ttctggcttg tctccccaaa
      961 tatgggcaac catcggctgt tcatcttctg taaatgttaa tcttcctcgt acactgtctt
     1021 ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc agtaaagaag acatccggtg
     1081 ctgctgcatg ctttacgaca tggcgaaata caacatctgt tacatcttcc attggtgcta
     1141 aaacaaaaaa aggctttggc agctctttcc aaaaattagt agtcatttat ttgttactct
     1201 ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa cattgtcatt atactaggaa
     1261 acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc tgattgagca aaaaaaagac
     1321 tgtgacaaaa atcttatcac agtccatttt ccggaataaa tggtgttcaa aagcttgcat
     1381 ttgcggtaat aag
//
LOCUS       DACEMK010000204         1377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEMK010000204 DACEMK010000000
VERSION     DACEMK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.214"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..>1377
                     /locus_tag="IW715_002705"
     CDS             50..>1377
                     /locus_tag="IW715_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1023270.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKY"
BASE COUNT          541 a          175 c          236 g          425 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatat
//
LOCUS       DACEMK010000205         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEMK010000205 DACEMK010000000
VERSION     DACEMK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.215"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>1368
                     /locus_tag="IW715_002706"
     CDS             82..>1368
                     /locus_tag="IW715_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1023271.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DACEMK010000206         1365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEMK010000206 DACEMK010000000
VERSION     DACEMK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.216"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            76..1041
                     /locus_tag="IW715_002707"
     CDS             76..1041
                     /locus_tag="IW715_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1023272.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          443 a          215 c          306 g          401 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aaaatttgag taaacgtggc acgatcataa atgagtggga aaatttaaca
      121 gccctatcta acaggcggtt aagtgtgcaa actttgacca ccccccttaa aatacagtgg
      181 tcaattgacc gaattaccat tgttggaaaa ctcaaagaga atatttatta tcacacgcaa
      241 aatgatgtat tgattttaga ctttgaacag ttaatgcgac tgaatgaaaa taatggttat
      301 gttaaatctg ttggtctaaa tggttggcaa attgtggata agcatgatga gaatattgct
      361 tacattgaaa ttttgaaata tcaagaaggg caagggcgaa ttgactttaa cccaaataaa
      421 attagccaat ttttggcggg ttcgatgaag aactttatcc atgatttatt tttagaacca
      481 catttctcac gtgctgatat tgcttgcgac attatcgatg tgcctgatga ttttattacg
      541 caatatcgtg tggttgaccc tgtttcattc aaaccaatct atgggcggtc tgggaagctg
      601 gaaacggcat attggggtag tcgtgccagt gagcgacaaa taaggatgta caacaaaaaa
      661 ttggaacaag aaaccaaacg gaaaattgta ccaccggaaa taaaaacttg gtggcgactg
      721 gaattgcagt tacggcgtgg aaaagctact gattggtatg cgatggttca tgaaagttta
      781 gacagtttcg caagtcctca ttttttgccg actgatacaa agcatactga tagaattatg
      841 attgcaggat tgatttcaga tcaaaattta tgggctggct tggctcgtca tacaaaatat
      901 aaatatcgaa atttgttgaa aaaagaaagc caaagtgatg agttagttaa tcatttacga
      961 gagacatttt cggaatcggc agatgttttg aaaaaagaat tggatacgtg gcttttgggg
     1021 ttggacgtga cggaagaatg agtggatgaa tttttgtcca cttttttatt ttcagcacaa
     1081 gtcggcatgg tgggtcgtgc gtttgcagca agagcgaaaa aacgctaacg aatccgcaaa
     1141 tgccgacttg gataaaacag ttgcgcaaag agacttggct ttttataata aatttaggga
     1201 tatcccagtg tcactctttg gggtaattcg ggggaaggag ttacccctca ctctggaaaa
     1261 ttagagcgaa gcagacgaga ttatcccaat gaatatgacg gcttactcgg agtgataccg
     1321 agtaaaaaag tcagataagt gagagtaaat ttacgtcaat gttta
//
LOCUS       DACEMK010000207         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEMK010000207 DACEMK010000000
VERSION     DACEMK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.217"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1355
                     /locus_tag="IW715_002708"
     rRNA            <1..>1355
                     /locus_tag="IW715_002708"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACEMK010000208         1311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEMK010000208 DACEMK010000000
VERSION     DACEMK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.218"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..1248
                     /locus_tag="IW715_002709"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IW715_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACEMK010000209         1298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEMK010000209 DACEMK010000000
VERSION     DACEMK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.219"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1298)
                     /locus_tag="IW715_002710"
     rRNA            complement(<1..>1298)
                     /locus_tag="IW715_002710"
                     /product="16S ribosomal RNA"
BASE COUNT          274 a          400 c          301 g          323 t
ORIGIN      
        1 acgacttcac cccaatcatc tatcccacct taggcggctg gctccaaaag gttacctcac
       61 cgacttcggg tgttacaaac tctcgtggtg tgacgggcgg tgtgtacaag gcccgggaac
      121 gtattcaccg cggcgtgctg atccgcgatt actagcgatt ccggcttcat gcaggcgagt
      181 tgcagcctgc aatccgaact gagagaagct ttaagagatt agcttagcct cgcgacttcg
      241 caactcgttg tacttcccat tgtagcacgt gtgtagccca ggtcataagg ggcatgatga
      301 tttgacgtca tccccacctt cctccggttt gtcaccggca gtcttgctag agtgcccaac
      361 tgaatgatgg caactaacaa taagggttgc gctcgttgcg ggacttaacc caacatctca
      421 cgacacgagc tgacgacaac catgcaccac ctgtcacttt gcccccgaag gggaagctct
      481 atctctagag tggtcaaagg atgtcaagac ctggtaaggt tcttcgcgtt gcttcgaatt
      541 aaaccacatg ctccaccgct tgtgcgggcc cccgtcaatt cctttgagtt tcaaccttgc
      601 ggtcgtactc cccaggcgga gtgcttaatg cgttagctgc agcactgaag ggcggaaacc
      661 ctccaacact tagcactcat cgtttacggc gtggactacc agggtatcta atcctgtttg
      721 ctccccacgc tttcgagcct cagcgtcagt tacagaccag agagccgcct tcgccactgg
      781 tgttcctcca tatatctacg catttcaccg ctacacatgg aattccactc tcctcttctg
      841 cactcaagtc tcccagtttc caatgaccct ccccggttga gccgggggct ttcacatcag
      901 acttaagaaa ccgcctgcgc tcgctttacg cccaataaat ccggacaacg cttgccacct
      961 acgtattacc gcggctgctg gcacgtagtt agccgtggct ttctggttag ataccgtcaa
     1021 gggatgaaca gttactctca tccttgttct tctctaacaa cagagtttta cgatccgaaa
     1081 accttcttca ctcacgcggc gttgctcggt cagactttcg tccattgccg aagattccct
     1141 actgctgcct cccgtaggag tttgggccgt gtctcagtcc caatgtggcc gatcaccctc
     1201 tcaggtcggc tatgcatcgt ggccttggtg agccgttacc tcaccaacta gctaatgcac
     1261 cgcgggtcca tccatcagcg acacccgaaa gcgccttt
//
LOCUS       DACEMK010000210         1279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEMK010000210 DACEMK010000000
VERSION     DACEMK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.220"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..863
                     /locus_tag="IW715_002711"
     CDS             <1..863
                     /locus_tag="IW715_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1023273.1"
                     /translation="EKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKIN
                     NLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDR
                     EKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQ
                     RELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFS
                     EDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQ
                     YRRSLELSKD"
     gene            935..>1279
                     /locus_tag="IW715_002712"
     CDS             935..>1279
                     /locus_tag="IW715_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023274.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          566 a          147 c          208 g          358 t
ORIGIN      
        1 gagagaaaaa taatttaaag aaaaaaatgc tcattccatt tgattcagaa gtgaaaggat
       61 tactcaataa actaactatt tctaatatag actcgactag ggaaaaaatc ataaaaatat
      121 ttgataaaat taataattta ttcaagaatg acaatgctga attaaggaag ggtcttttag
      181 agtttaaact tcaagaacta gaacttcatt actcttattt agtgaaaatt accgaagaaa
      241 aagaacaaca aaaagcaata aaagaacaga tgatagaaga ggaaaaagtt cgtagagaaa
      301 ttgatcgtga aaagaaacgt attgataaag aacaacgtca attcaattct gaaatttcca
      361 aattaatcgc atatatgcaa aaagctaatg cagatgtgga aaaagaatta tatgcaaata
      421 aaattcaaga actggaagaa aaattaaaag aattagaagt tatacgtgaa aatgtattac
      481 aaagagaatt aaatactaga gccggttatg tgtatgtaat tagtaatatt gggtcttttg
      541 gtgaagacat atataaaatt ggtatgacta gaagattaga acctatggat agaataaaag
      601 aattgagtag tgcatcggtt ccatttgaat ttgacgttca tgcgatgatt ttttcagagg
      661 acgctccgtc tttggaaact aaattgcata atcattttag aaaacaagaa gttaataaaa
      721 ttaatcaaag aaaagaattt ttcaaagttt cactagacga aatagagaaa gttgttttag
      781 aaaattataa tggaacagta acatttacaa aattagctaa agctgaacaa tatagacgat
      841 ctttagaatt gtcaaaagat taaatatata aaaagccttc gggctttttc ttttaacaat
      901 taataggaac gtatattcga aaggaataga aataatgtgg ttagaaaaaa taaatgacaa
      961 aaaatttaag tacaccgaac gttatacgga tcccttaact gaaaaaaaga gaaaagtgtc
     1021 cgttacttta tctagcaatt cacgccaagc ttggaatcaa gctaacttac ttttgaatga
     1081 aaaaatcgca gaaaaaataa aacgaaatga agaattacca ctaacttttg gagaattaaa
     1141 aactaaatgg gatgaaaaat acaagccaac tgtaaaagaa agttcctatc gaacaactca
     1201 ggtttatcta tcattaattt caaaatatat aaaagatgat gtacttgtaa aaaacgttaa
     1261 tagtaatctt atacaagat
//
LOCUS       DACEMK010000211         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEMK010000211 DACEMK010000000
VERSION     DACEMK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.221"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..532
                     /locus_tag="IW715_002713"
     CDS             35..532
                     /locus_tag="IW715_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1023275.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IW715_002714"
     CDS             complement(569..1252)
                     /locus_tag="IW715_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023276.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACEMK010000212         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEMK010000212 DACEMK010000000
VERSION     DACEMK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.222"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(62..280)
                     /locus_tag="IW715_002715"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IW715_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IW715_002716"
     CDS             complement(546..740)
                     /locus_tag="IW715_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023277.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          450 a          214 c          174 g          438 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaatt
//
LOCUS       DACEMK010000213         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEMK010000213 DACEMK010000000
VERSION     DACEMK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.223"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW715_002717"
     CDS             414..947
                     /locus_tag="IW715_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1023278.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEMK010000214         1211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEMK010000214 DACEMK010000000
VERSION     DACEMK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.224"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            10..210
                     /locus_tag="IW715_002718"
     CDS             10..210
                     /locus_tag="IW715_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ1023279.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="IW715_002719"
     CDS             288..962
                     /locus_tag="IW715_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1023280.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
BASE COUNT          422 a          159 c          257 g          373 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt t
//
LOCUS       DACEMK010000215         1198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEMK010000215 DACEMK010000000
VERSION     DACEMK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.225"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(28..267)
                     /locus_tag="IW715_002720"
     CDS             complement(28..267)
                     /locus_tag="IW715_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023281.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(381..722)
                     /locus_tag="IW715_002721"
     CDS             complement(381..722)
                     /locus_tag="IW715_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1023282.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(709..1038)
                     /locus_tag="IW715_002722"
     CDS             complement(709..1038)
                     /locus_tag="IW715_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023283.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
BASE COUNT          342 a          270 c          142 g          444 t
ORIGIN      
        1 agcctatgat cacatacgct ctacacttca gtcttgggtt atctttttct ttcgtttaat
       61 tactggccgt gctggtttca aatccttgat tccattatgg gttctccaat aatcgaattc
      121 ttttcgtaat ctttcttttt ccatatctgt tcctttagac agggatttga atacatcttt
      181 aggtgaataa ccacgagcta acagctgtac ataccttcct tttgtcaaag tatccatatc
      241 catcgcctct aaaactggtc gcgccatcta attttcctcc taaataaatt ccttactcaa
      301 tgcctaaggt aatactacct ttttattatt cgtcgtgact ccttctaagc ctttatctgt
      361 aaaatttatt cctccagttt ttattcttct ttatccgcta caaattcaat gatttcaacg
      421 ctgacaatat aatcactcaa atatgaccaa gtatcttcaa cagataattc gtctccatta
      481 tcatccgtaa gcgtcatttt aaaccattca ttaccttcat catcttcagg tagcaagccg
      541 aatccaattg gattatcagt caatgtttga tcgtataaat catcctcgtc taatgctaaa
      601 acatctaatc ctttacaatt tccgcctctt ctaaacgtca caaatccttt gtaatcccaa
      661 cgttggaaag aaaccttaat cgtatgtgtg cccaatcgta tattcggatc ataatttttc
      721 atttgttgta acctcctcat aatcagttag tactataaag gtatctttgt ttttatccaa
      781 aaaatctaat aaatcttttg tagaagatat ctcttcatca acttcaccat aataaatacc
      841 ttcgataatt gttgctatat ctgaaaaact aatttttact ggacatttat cgtctaccgc
      901 attcaatacc gacaaaattg cttctttttc tgcaatctct cgtttgaatt tctttggagc
      961 taatcgcatg acactcaagt cattttcaag cttttcaatc catgcagttt tactcatttt
     1021 cgcaaatttt tgaatcattc ctctaccacc tttgctatct actaaattag ttttacttca
     1081 taacgaatat ctttatcgtt tttcgttcac tcaaaaatat ataaaataaa tcgactcgtt
     1141 tatggcgtcc gaaacgagaa accctagtag acagcggtaa atgctcgatt gatccaat
//
LOCUS       DACEMK010000216         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEMK010000216 DACEMK010000000
VERSION     DACEMK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.226"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1076
                     /locus_tag="IW715_002723"
     CDS             <1..1076
                     /locus_tag="IW715_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1023284.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACEMK010000217         1188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEMK010000217 DACEMK010000000
VERSION     DACEMK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.227"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..164)
                     /locus_tag="IW715_002724"
     CDS             complement(<1..164)
                     /locus_tag="IW715_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAQ1023285.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDS"
     gene            complement(175..>1188)
                     /locus_tag="IW715_002725"
     CDS             complement(175..>1188)
                     /locus_tag="IW715_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1023286.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
BASE COUNT          319 a          258 c          186 g          425 t
ORIGIN      
        1 gaatctaact cgactaaatg accaaaacca tacgtgatca cagtttctcc tgtaaacaat
       61 cgatcttgga tctcaaaata cccatctttt cgggtagctt tagaaaagct atcggcataa
      121 gccttcgctt gtgacggttt ttctgctaaa ataacggttt tcatcatgac gatttcactc
      181 cctttcttgc tgtctacctt gttgaaacaa catggcacgt ttttgctttc tgatctcttg
      241 ttggtagatt tcatgcttca attgatcatt ccaatccttt tttacttcag gtacaaattc
      301 atgttcatga tttttatagc ctttgtagta ttgttctgct ttataagaga aaagaatctg
      361 agcattaata tccccttttt cttgataaaa acgatcaaaa tcgtacgtac catccacgcg
      421 ttcacaagct tttaattctt ttgcgacttc tctacaaata tctttcaaat taatagcttc
      481 tattgaatcg ttaacagaaa gctgctgacc aaaagacgca tttactttgt gagcggaaac
      541 caattggttc gttaattgca gattgttttc tgtcttgtgt atggccataa tagcttctgg
      601 tgtgatcttt tcttcttttg cgacttcttc ataaatgact ttgtattctt ttggcacttg
      661 ccaatcatta gcgattcctc gttcacaacg tactttctga ttcgtaaatg gatcaactgc
      721 gcccattaat tgttcttttt cgtagaaaat atgtcctgca cggtcattat ccacacaaat
      781 ctcgatggat tgaggaaagg cttgtttctt tctcaattca gatactgcct caccaaaata
      841 atgactgatc acatggtgtt ttaacccttc catcgaaact aaccacgtat cgtttaactg
      901 atcacgattt agtgcagcat agctaagtaa atcaatacta ctctcaaaaa attttaactg
      961 cttagggtca ccgatcttta ggttaaagcc atgattcgct gtcgaatttt tatctatatg
     1021 cttatatgtg cctctctcac caaaacgttc ataatcaatg aacgttcctt gataactcgc
     1081 ccctactgtc tcatttgtct gtggatcttt ccataaaaag gcgaccgcat gatctaaacg
     1141 atcgtctcca actaagaaat tttgccgttc atgttttggc tttagttc
//
LOCUS       DACEMK010000218         1162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEMK010000218 DACEMK010000000
VERSION     DACEMK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.228"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            191..445
                     /locus_tag="IW715_002726"
     CDS             191..445
                     /locus_tag="IW715_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1023287.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IW715_002727"
     CDS             446..763
                     /locus_tag="IW715_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1023288.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          411 a          176 c          231 g          344 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tg
//
LOCUS       DACEMK010000219         1134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEMK010000219 DACEMK010000000
VERSION     DACEMK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.229"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..>1134
                     /locus_tag="IW715_002728"
     CDS             join(55..331,331..>1134)
                     /locus_tag="IW715_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1023289.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          425 a          192 c          217 g          300 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acac
//
LOCUS       DACEMK010000220         1105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEMK010000220 DACEMK010000000
VERSION     DACEMK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.230"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..974
                     /locus_tag="IW715_002729"
     CDS             <1..974
                     /locus_tag="IW715_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023290.1"
                     /translation="RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEE
                     NDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTG
                     LSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDES
                     KYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIF
                     LSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLI
                     NNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            984..>1105
                     /locus_tag="IW715_002730"
     CDS             984..>1105
                     /locus_tag="IW715_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1023291.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSG"
BASE COUNT          450 a           94 c          186 g          375 t
ORIGIN      
        1 aaagaataaa tagagaagat ttatctgacg aatttaagaa tgttgtttca ctcgaagcta
       61 caaataatac taaaagaaat attttattta gtagtggagt atttacaatt aaagaaggta
      121 aaaatataga agaaaatgat aagaattcaa ttattgttca tgaagaattt gctaaacaaa
      181 acaatctaaa attaggtgat gaagttgatc ttgaattact agatattgaa aaaagtggaa
      241 aaataaaaag tcataaattt aaaattatag ggatcttttc tggtaaaaaa caggaaacat
      301 atacaggatt atcttctgat tttagtgaaa atatggtgtt tgtagattat tcaacaagcc
      361 aagagatatt aaataattca gaaaataatg aaattgcaaa taaaatttta atgtattctg
      421 gtagtgcaga atctacagac ttagccttaa acaaattgaa agagcttaaa attgatgagt
      481 caaagtattt tgttgaaaaa gattctaatg cgtttgaaga gtctttagag tcagtgagtg
      541 gaataaaata tataataaaa gtaatgactt attctattat gttaggtggg atggttgttc
      601 tttcattaat cttgattcta tggttaaggg aaagaattta tgaaataggt atattcttat
      661 ctattggaac atctaagata caaattataa tgcaatttat attcgagtta atattcatat
      721 cgataccaag tataatatct tccttatttt taggaaatgt attactaaaa gtaattgtag
      781 atggattcat taactcagag gattcaatga tttccggtgg aagtttaata aataatagta
      841 gttttatgtc aaatataaca acacttggac aaagttattt aatattaata agtattattg
      901 ttttatcagt tgtatttgct tcttcattaa tgttaatcaa gaagcctaaa gaaatcttat
      961 caaaaataag ttaggagaaa ataatggata tattagaaat aaagaatgta gcatatagtt
     1021 atgcaaattc taaagaaaaa gttttgtcag gagtaaatca aaaatttgaa cttgggaagt
     1081 tttatgcgat agtaggaaag tcagg
//
LOCUS       DACEMK010000221         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEMK010000221 DACEMK010000000
VERSION     DACEMK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.231"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..980
                     /locus_tag="IW715_002731"
     CDS             6..980
                     /locus_tag="IW715_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1023292.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACEMK010000222         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEMK010000222 DACEMK010000000
VERSION     DACEMK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.232"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            700..>1082
                     /locus_tag="IW715_002732"
     CDS             700..>1082
                     /locus_tag="IW715_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1023293.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMW"
BASE COUNT          413 a          161 c          244 g          264 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 ga
//
LOCUS       DACEMK010000223         1060 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEMK010000223 DACEMK010000000
VERSION     DACEMK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.233"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(92..433)
                     /locus_tag="IW715_002733"
     CDS             complement(92..433)
                     /locus_tag="IW715_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1023294.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          314 a          245 c          129 g          372 t
ORIGIN      
        1 tccttactca atgcctaagg taatactacc tttttattaa tcatcgtgac tccttctaag
       61 cctttatctg taaaatttat tcctccagtt tttattcttc tttatccgct acaaattcaa
      121 tgatttcaaa gctgacaata taatcactca aatatgacca agtatcttca acagataatt
      181 cgtctccatt atcattcata agcgtcattt taaaccattc attaccttca tcatcttcag
      241 gtagcaagcc gaatccaatt ggattatcag tcaatgtttg atcgtataaa tcatcctcgt
      301 ctaatgctaa aacatctaat cctttacaat ttccgcctct tctaaacgtc acaaatcctt
      361 tgtaatccca acgttggaaa gaaaccttaa tagtatgtgt gcccaatcgt atattcggat
      421 cataattttt catttgtcat tcctccatta cgtaaatttc attttctcta tgttttatat
      481 caattttttc actttattca ggacttgttc atattttatc gtttttcgtt tacttaaaaa
      541 tacataaaaa atttcgactc gtttatggcg tccgaaacga gaaaccctag tagacagcgg
      601 taaatgctcg attgatccaa taatcaacgt cctcgcaagt atctacatgt actgcagcac
      661 acgacacaat ttcttgtgcg tcctccccat tacgagaaga cctactcctt attccatgag
      721 tttgcgtcaa agtctagatc ccttagtagc tgctcatgat taatctagac gctattctaa
      781 cttgtctaac ttctaaattc cctcttgttt actctgcgct tattatttac acaaattcat
      841 ttcaccacta tccaaacaag cgcatttact cgattgacca attttttacg ttaccaatcg
      901 ttaaaaccta ccccgtcact ccccgaaggg aattgttgat agatcaagtc tactccttat
      961 ttttctagtg actctaagtt cctttagaag tctcgacaat attttcaagc aagcgaaaaa
     1021 cgataaaata caagttatga gaatgatcaa ttcagccgat
//
LOCUS       DACEMK010000224         1011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEMK010000224 DACEMK010000000
VERSION     DACEMK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.234"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..191)
                     /locus_tag="IW715_002734"
     CDS             complement(<1..191)
                     /locus_tag="IW715_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1023295.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKY"
     gene            complement(188..841)
                     /locus_tag="IW715_002735"
     CDS             complement(188..841)
                     /locus_tag="IW715_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1023296.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          323 a          173 c           92 g          423 t
ORIGIN      
        1 atatatttta tttcttttcc attcatatta ctagaaattt catttgccat attatgaatc
       61 ttttcttttc tcgtctccaa atatgtttta ggaaagataa aaaaaattaa tgaatgaact
      121 aatataatta ttattccaag aacagaaaat atttgtatga acatttttgg aaatatcttt
      181 aattttttca ttttctctcc aatttatatc ctacatttct tatagtagtt atacagtcta
      241 attgtaattt ttttctaagt tcttttatat atacatctat tactcgatca taaggagttt
      301 cttcgctatc tttccataca taatcaataa tttgcattct tgttaacact tgtccattat
      361 tatccaataa acattttaat acttctagtt cttttgcatt tacatctatt tcttcaccat
      421 ttattttagc tgtatagcta ttaaaattaa ccgatagatc cttatattca aacttctcta
      481 aatgtccata attcttctta attaaagaat ctattctagc ttttaagacc ggcaatgaaa
      541 atggcttttc tacatatccg tctactaaat tagtaaatgc atcaatttta tattcttcat
      601 cactaaatgc agtcaagatt agaattggta aattgctttt ctttctaatt tctttcaaca
      661 cttctaaacc atttataaaa ggtatctgaa tgtctaagat aaccaaattt atatcgctat
      721 taaattttga caaggcttct cttccatctt tagcttgaat aacagtatat ccaaattctg
      781 aaagatattc acttattccc tctcttatca tattatcatc ttcaacagtt aatattttca
      841 tatatacctc cacttaagcc tatacactag atattataca gcatattatg gctctataat
      901 atctagtgtt ggtgacgcct tctgttgcca atactttttc ttttttttaa taaatgcaaa
      961 cagacacctt gaaatccttt agaaaaaagg cttctataat attttgtgtt g
//
LOCUS       DACEMK010000225          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEMK010000225 DACEMK010000000
VERSION     DACEMK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.235"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            264..965
                     /locus_tag="IW715_002736"
     CDS             264..965
                     /locus_tag="IW715_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1023297.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          363 a          164 c          156 g          315 t
ORIGIN      
        1 ttgaaaaaat ttttttgaat aaattaaaaa aataaattat attatcgttg ttagcgttta
       61 ctatatgtat tattgttgtt ttttatacac aaatcattcg cttcaaaaca aattattcaa
      121 taccattgta tattttttac ataataacaa attatatttg taaagtatta aaacttgttt
      181 ctaatgttgg tatgttaaaa ctttacaaat tctttaaaaa aagtgacaat aaatatagtg
      241 aacaataagg agaggacttt ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac
      301 tcagatatca acctgatttt gaacatttct cggatgaaga gttccaacag atcaaggaac
      361 atactgttct tcgttcttat aaaaaaggac agatcctttt cgacgatggt gatcccagac
      421 aacggttgta tatcttgacc aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa
      481 cttattacta ctatgactat gtgaatacaa ataacctttt tcctatgtca ggaatgtttg
      541 gagaagagac ctacacttat acagctcaag cgatgactga tattgaaaca ttctaccttc
      601 ctgtttccta ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc tatatgatac
      661 aaaaaatttc taaagtagtt gaaatacatg aattgcgtat ccaaatcgga ttgacctcta
      721 gcgcctttga ccgggtaaaa caaaatttat tcattttgaa agaagaattg ggcgtaccaa
      781 caaaagatgg tcatatcacc attccttatc cgatcacctt aaaagaatta gcagtgaata
      841 gcggcacgac acgtgaaact gctggacaag taatcaaaca actacgtgat tccgggctgt
      901 tagattatga caaaaagcgt tttactttct gtaagacagc tactactcca gctatgagag
      961 aataacgcga acaaaaataa gcagtgaccc tgataaat
//
LOCUS       DACEMK010000226          985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEMK010000226 DACEMK010000000
VERSION     DACEMK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.236"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..888
                     /locus_tag="IW715_002737"
     CDS             <1..888
                     /locus_tag="IW715_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1023298.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          417 a          129 c          164 g          275 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc aatgaggggc ggtacatata taaattagcg catattatta gctaatttgt
      961 aatcaaaagc agcgtaaact agttt
//
LOCUS       DACEMK010000227          920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEMK010000227 DACEMK010000000
VERSION     DACEMK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.237"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..803)
                     /locus_tag="IW715_002738"
     CDS             complement(<1..803)
                     /locus_tag="IW715_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1023299.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYT"
BASE COUNT          290 a          165 c          114 g          351 t
ORIGIN      
        1 gtatagttat ttttaatcat tgtcatcatc atccttattg cttcatagac atagcgagtt
       61 ctaacattaa ttgataattc cggataaaac ttatctatga atattagtat atcttcatgg
      121 acttcaaaag aatcaaaaat tctaggattg aagcttcctc tcagtatact tttagggctt
      181 tggtaatagt gatatttagc agatgaattg acagcaactt tttcggaatg atcgatcagc
      241 aaataagtag tagccttatc ttccattaat ttacccagtg ggaatcttat attatcaaat
      301 aaatttctat tgaataattt attatttgca gaatagccaa ttttattatt tataaataag
      361 tttttcattg cttgttctct atcaaatatt tcccattttc tctcagcaat tgtatgttct
      421 tttagttgtt ctaaacttga aatctttttt atttttagca ccgagatatc tgctccataa
      481 gtttgcgtgc caaaatatag taactcaaga tagtctggtt ctatccaatc atcgctgtca
      541 ataaagcaca caaaatctcc tgttgcagaa tcaagaccag aattcctagc cgcacttaaa
      601 ccaccattca cttgatgtat cacttttaat cgcatatctt tctttttata ttcttctgca
      661 atttccccac ttcgatccgg ggaaccatca ttgactacta ttatttcaat atttgaatgt
      721 gtctggttgg aaatagattc taagcagttt tctaaatagt cttctacgtt atatgcgggt
      781 actataacac taattttatg cactctcttc acttcctaaa aatcatcttt tttattttta
      841 caaatgtatc attaccagta acaattttag tcttccgaag ttaataaaca taaaaaataa
      901 catatcgaaa aaaactttat
//
LOCUS       DACEMK010000228          902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEMK010000228 DACEMK010000000
VERSION     DACEMK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.238"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..858
                     /locus_tag="IW715_002739"
     CDS             <1..858
                     /locus_tag="IW715_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1023300.1"
                     /translation="SAIISLIYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVG
                     ELKQQINDLYSTLLRTIDDLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILEN
                     MKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDIL
                     KKYEILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVND
                     WLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEF
                     FIFKIKIFS"
BASE COUNT          404 a           63 c          123 g          312 t
ORIGIN      
        1 tctgctatta tttctttaat ttatgcaaaa tcaataaaaa ataatataca agaaataaaa
       61 attgttactg ataaaatgat gaaacttgat aaaaaaatga gtttaaaagt tagctctaat
      121 gatgaagttg gagaattaaa acaacaaatt aatgatttgt attctacttt attaagaaca
      181 attgacgatt tggaatttaa aaataaagaa attttaaaat tagaaaagtt aaaatatgac
      241 ttttttaaag gtgcatccca tgaacttaaa acccctcttg ctagtcttaa aataatactg
      301 gaaaacatga aatacaatat tggaaaatat aaagaaagag atatttatat taatgaatgt
      361 attgaaatcg ttgatagttt aacaaaaaat atttctcaaa tattatcagt tcattctata
      421 gaaaatttga ataatgatga agaatatttg aaaataaatg atacattaga agacatatta
      481 aaaaagtatg aaatattagc aaatcaaaag aaaataactg tcaataatta tatattagac
      541 gagaatgttt atataggaaa aacagcttta aagattattc tatctaattt gattagtaat
      601 gcggtaaaat atactgatgt aaatggggta attaatattg ggatagttaa tgattggtta
      661 tatatagaga attcatacgg taacaataaa atttcgaata tggataaaat atttgatgtt
      721 aaatttgatt taaataagga aaatagtaat ggattaggtt tatatattgt aagtaatatt
      781 ctgaataatt acaatataga atataaagta ttgcaggatg aagaattttt tatctttaaa
      841 ataaaaatat tttcttaatt aagatataat tttgcaatag ttattgaaaa atagagactt
      901 at
//
LOCUS       DACEMK010000229          888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEMK010000229 DACEMK010000000
VERSION     DACEMK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.239"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..378)
                     /locus_tag="IW715_002740"
                     /pseudo
     CDS             complement(1..378)
                     /locus_tag="IW715_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            454..609
                     /locus_tag="IW715_002741"
                     /pseudo
     CDS             454..609
                     /locus_tag="IW715_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            628..>888
                     /locus_tag="IW715_002742"
     CDS             628..>888
                     /locus_tag="IW715_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1023301.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYD"
BASE COUNT          291 a          116 c          164 g          317 t
ORIGIN      
        1 gtttcgcttg ataatagtag gtctgacgag aaatatttag aattttgcac atcgctgata
       61 tggaatattt atgcttgttg gcatcaatta cttgtctttt cgtccgaata tcagcgccgc
      121 ttgctttaaa atatcattct ccatttcgag ttgcttattt ttctttctta gtgcgatcaa
      181 ttctgcttgt tctggtgtta agttgtctct ttctttgaat gagcccgttg attgtgcttg
      241 cttcatccat ttatcgaaag aagaaggcgt aagctcatac tctcgaataa tttctgcgcg
      301 aggcttacca gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct
      361 tcgttgacgt cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta
      421 ttttttctgt ctagtctagt gtagccgatt caggataagg ataagtgtat taattgtgga
      481 aaatgtaaaa aagtttgcca aatgaataaa gattcagttg agaattgcaa tcatctggag
      541 tgtatcagat gtggacgatg taaaaatgct tgtccagtgg atgcaattag ttatggagta
      601 agaaaataga gaggagtagt taggaaagtg ataaaaaatg ctatagcata tattacaaga
      661 aagaaaaata gaactttaat aatttttatc atattaacta tagttctttc ttgcttgtat
      721 tcttgtttaa caataatgaa atcaagcaat gaaatagaaa aggctttata tgaaagttct
      781 aattcttcaa tatcaataac aaaaaaagat ggtaaatatt ttaatgttaa tcaatttaaa
      841 gatattgaaa aattaaaaga aattgaagaa aaaataattc aatatgat
//
LOCUS       DACEMK010000230          868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEMK010000230 DACEMK010000000
VERSION     DACEMK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.240"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..856
                     /locus_tag="IW715_002743"
     CDS             <1..856
                     /locus_tag="IW715_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1023302.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIR
                     HGKTFLTK"
BASE COUNT          279 a          174 c          166 g          249 t
ORIGIN      
        1 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
       61 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      121 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      181 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      241 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      301 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      361 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      421 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      481 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      541 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      601 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      661 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      721 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      781 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
      841 atttttaacc aaataaacag aacagaaa
//
LOCUS       DACEMK010000231          864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEMK010000231 DACEMK010000000
VERSION     DACEMK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.241"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(255..554)
                     /locus_tag="IW715_002744"
     CDS             complement(255..554)
                     /locus_tag="IW715_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1023303.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          289 a          148 c          102 g          325 t
ORIGIN      
        1 ctatatttcc aatttttaac ttacaaaaat gcaacattaa taaataacga tagtttcata
       61 aaatacactt ttaattaaca ttattagttt atactagctg tttttgtgtt aattaaagga
      121 taaaatacaa tataaagaaa catctgtttc tttatttaga ataaaaaaat acagactctg
      181 ttcaaataaa tgaacctagc ctgcattatg agattgacta tactttttaa ttcattgaga
      241 tgaataactc gtttttataa atgcccaaaa gtaatcggtg ccgaagccat tcctcgacct
      301 aattcttttt caaattttcc tattgttgat atctccatat aaaacggtga tactggttta
      361 gacaattctc ccctgattgc taacggacgt aaactacgtt ccaaattatt tatgacttta
      421 ggataatact gacctttttc taagcctgtc tttgcgtcta tcaaaagtat acgttccttt
      481 tctttgatct ctggatttaa aacgagatca tataatttta gtaccatgtt ttctatttct
      541 tcacttttag acatcacgat ttcctcccaa aaatatattt cttcccacta cattctataa
      601 acaaagtata cgctacatct agattttttt aaatcattac tggattgtta aaacctttca
      661 atacactgtt atggcaacga tttttatttt aaaaacattc taaattaaat cagtaaaaat
      721 ttttattcta agatttttat tagaattacg cctaatttgt gtctctcagg gcaattctaa
      781 gaacgtttca agagatagcg acactatcgt tatcgaaaaa ggcgaaaacc tatctcattt
      841 tctgctaatg taagttggtt tagc
//
LOCUS       DACEMK010000232          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEMK010000232 DACEMK010000000
VERSION     DACEMK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.242"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..716
                     /locus_tag="IW715_002745"
     CDS             24..716
                     /locus_tag="IW715_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1023304.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DACEMK010000233          790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEMK010000233 DACEMK010000000
VERSION     DACEMK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.243"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..782
                     /locus_tag="IW715_002746"
     CDS             <1..782
                     /locus_tag="IW715_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1023305.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc
//
LOCUS       DACEMK010000234          772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEMK010000234 DACEMK010000000
VERSION     DACEMK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.244"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..204)
                     /locus_tag="IW715_002747"
     CDS             complement(<1..204)
                     /locus_tag="IW715_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1023306.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMK"
     gene            complement(201..425)
                     /locus_tag="IW715_002748"
     CDS             complement(201..425)
                     /locus_tag="IW715_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1023307.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(422..715)
                     /locus_tag="IW715_002749"
     CDS             complement(422..715)
                     /locus_tag="IW715_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023308.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
BASE COUNT          223 a          158 c          122 g          269 t
ORIGIN      
        1 tttcatgttg gcggctacgt ttcttgctgg tgcatctatt ccagtttcgt gcaaaataat
       61 tttgttagga attgctaatc tggagtctcc ttgattcggc gctaaattat actcgtcatt
      121 aatagtataa gcaaacgtat taatgggtaa taaaaaaaga gccgttaaca ggctcatcgc
      181 agtaatagta attttctttt tcatttgttt cctccttctt cgctttcagc cgagaacatt
      241 ttgtaggttc gatttgatac acccaacaca ctccctaaaa acgcgccaaa accagtaatg
      301 atgacaacac agatatctgt gtactgccaa ttgagcgctt taccaactaa ccccacgaaa
      361 gtagctagtg cgggaataat taccagtgcg aaccatttta gtacttcgaa cgttttatta
      421 ttcattttct tctctcccta aataaagttt taatttgttg cgtgtgttct accaattttt
      481 ctgcatgtgt atctaatctt tcatcgtgtt tctttagttc ttcatgaatc atcaatcgat
      541 ctgatttgct cgattctaaa tctttagtca gcaaatctaa attgtgactt acttttgaaa
      601 gagtctcagt aatcttcgag aaagatgcag taattggttt tattactaat aaaatcaaag
      661 aaacgatagc ggttattgat cctgctatcg ctccccattc ccctaaatta atcatgtgac
      721 aactccttga atcaaaataa aaagcacatc aattaagatg cgctctcttc tt
//
LOCUS       DACEMK010000235          758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEMK010000235 DACEMK010000000
VERSION     DACEMK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.245"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..305)
                     /locus_tag="IW715_002750"
     CDS             complement(<1..305)
                     /locus_tag="IW715_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1023309.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNN"
BASE COUNT          290 a          110 c           83 g          275 t
ORIGIN      
        1 aaattgttca tttttagttt aaaaaaaact ttatcaatat tatttgctct aaatggacca
       61 aataagtaat atctggtttt aaacatgtat agaagaaaca tctcattaaa atcaccagta
      121 aagaaatgga aatctttagt ttttttaata ttatcagtta gtaacttttt ataatcataa
      181 aaaaaataag tagttctatc tgagcgatac tcttttataa tactgaattc ggaattaaaa
      241 accacgatag gtaactttaa acaaacatga agagacttta ataagacttc tttatctgaa
      301 aacacttttg tttctctctt tcctttctac atcattaaat agtattttat acgttatcta
      361 aaatactatt cctctgtcaa aataagctaa cttacttcat tataaaatat ccacttatcg
      421 ctttcatgta tattccaccg taaaaagtac attttatcac cgagaactca tcatttttca
      481 tcatgatgaa tactatttac atatatctag ttcaaagaca ataattatta tgatccgtta
      541 ttatctaagc actatacaga taataggaat ttttcgtttc taaatattcc aatttgggaa
      601 tccaataaaa attttcgttc aaatacgtgt ctattcgaac tcttttctag ttgttaatta
      661 tgaaggggga agaaataaaa aatacaatct atacacgaat aagaatatta tccaaaagga
      721 ggttttagaa aagatttatt atacgtaaaa agctcaag
//
LOCUS       DACEMK010000236          756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEMK010000236 DACEMK010000000
VERSION     DACEMK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.246"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            312..608
                     /locus_tag="IW715_002751"
     CDS             312..608
                     /locus_tag="IW715_002751"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1023310.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLSTVCMKKLDVC"
     gene            complement(531..756)
                     /locus_tag="IW715_002752"
                     /pseudo
     CDS             complement(531..756)
                     /locus_tag="IW715_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          256 a          146 c          110 g          244 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatataa
      721 tgccactttt tccccacgcg aacataagta agatcc
//
LOCUS       DACEMK010000237          747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEMK010000237 DACEMK010000000
VERSION     DACEMK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.247"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            553..>747
                     /locus_tag="IW715_002753"
     CDS             553..>747
                     /locus_tag="IW715_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1023311.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          271 a          136 c          114 g          226 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattat
//
LOCUS       DACEMK010000238          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEMK010000238 DACEMK010000000
VERSION     DACEMK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.248"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>741
                     /locus_tag="IW715_002754"
     rRNA            <1..>741
                     /locus_tag="IW715_002754"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          151 c          220 g          144 t
ORIGIN      
        1 caaagagggt aaaaaccccg tagacgaaat gtggaagaca cctaggagga tcctgagtac
       61 ggcggaacac gagaaattcc gtcggaatcc gggaggacca tctcccaagg ctaaatactc
      121 cctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg
      181 agtgaaatag aacctgaaac cgtgtgccta caacaagtca aagcccgtta atgggtgatg
      241 gcgtgccttt tgtagaatga accggcgagt tacgattgca tgcgaggtta agttgaagag
      301 acggagccgc agcgaaagcg agtctgaata gggcgtttga gtatgtagtc gtagacccga
      361 aaccatgtga tctacccatg tccaggttga aggtgcggta aaacgcactg gaggaccgaa
      421 cccacgtacg ttgaaaagtg cggggatgag gtgtgggtag cggagaaatt ccaaacgaac
      481 ttggagatag ctggttctct ccgaaatagc tttagggcta gcctcggaat tgagaatgat
      541 ggaggtagag cactgtttgg actaggggcc catctcgggt taccgaattc agataaactc
      601 cgaatgccat tcattcatat ccgggagtca gactgtgagt gataagatcc atagtcgaaa
      661 gggaaacagc ccagaccacc agctaaggtc ccaaaatata tgttaagtgg aaaaggatgt
      721 ggggttgcac agacaactag g
//
LOCUS       DACEMK010000239          731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEMK010000239 DACEMK010000000
VERSION     DACEMK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.249"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW715_002755"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW715_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1023312.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IW715_002756"
     CDS             complement(481..675)
                     /locus_tag="IW715_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023313.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACEMK010000240          652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEMK010000240 DACEMK010000000
VERSION     DACEMK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.250"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>652
                     /locus_tag="IW715_002757"
     CDS             <1..>652
                     /locus_tag="IW715_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1023314.1"
                     /translation="NLDPLTTELVVMSRADNTGSGLILASSPEAKKRYGITNVSRPRD
                     LPQPFPKTLHVVPPRMNLYIKRNMQVNNIFRRYVADEDLLIYSIDESILKVTRSLNLF
                     TTEGTRSQRRKKLAQMIQENIKEELGLIATVGVGDNPLLAKLALDNEAKHNEGFVAEW
                     TYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRLGVIG"
BASE COUNT          225 a          109 c          142 g          176 t
ORIGIN      
        1 gaaatttaga tccattaaca acagaacttg ttgttatgag tcgagctgat aatactggtt
       61 caggattgat attagcttct tctcctgaag caaaaaagcg gtatggtatt acaaatgtga
      121 gtagaccacg tgacttacca caaccatttc ctaaaacact acacgttgtt ccaccacgta
      181 tgaacctata tatcaagcga aatatgcagg taaataatat tttcagaaga tatgtggctg
      241 atgaagatct actgatttac tcgatcgatg aatcaatctt aaaagtgacc cgatcactga
      301 atctttttac gactgaagga acacgaagtc aacggagaaa gaagctggct caaatgatcc
      361 aggagaatat taaagaagag ctaggattga ttgctacagt aggtgtcgga gataatcccc
      421 ttttggctaa actagcctta gacaacgaag caaagcataa tgaaggattt gttgcggaat
      481 ggacgtacga aaacgttcct gaaaaagtat ggaatattcc tgaaatgacg gacttttggg
      541 gaattggctc tcgaatgaaa aaaagactca atcagatggg gattatgagc attagagatc
      601 ttgctaactg gaatccttat acgattaaga atagactagg tgtgattggc tt
//
LOCUS       DACEMK010000241          635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEMK010000241 DACEMK010000000
VERSION     DACEMK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.251"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..278
                     /locus_tag="IW715_002758"
     CDS             <1..278
                     /locus_tag="IW715_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023315.1"
                     /translation="YASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEAEEFVYPF
                     RGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            355..>635
                     /locus_tag="IW715_002759"
     CDS             355..>635
                     /locus_tag="IW715_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1023316.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVP"
BASE COUNT          208 a          114 c          152 g          161 t
ORIGIN      
        1 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
       61 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
      121 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
      181 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
      241 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
      301 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
      361 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
      421 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
      481 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
      541 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
      601 agtcgtcaga tactctacta cagcttctgt gtgcc
//
LOCUS       DACEMK010000242          632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEMK010000242 DACEMK010000000
VERSION     DACEMK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.252"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            84..>632
                     /locus_tag="IW715_002760"
     CDS             84..>632
                     /locus_tag="IW715_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAQ1023317.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAI"
BASE COUNT          208 a          136 c          124 g          164 t
ORIGIN      
        1 atattttgtg taaatagaaa aaaggaagtc ccttctgtag aatagagtta ccacaacaca
       61 ttcacagaaa agaggacttc catatgaacg attttactac agaaattcta aagactctag
      121 cgaacaaagg cgatttgaat gaattattcc gtgtccattt ggaaaaagct gtcaatacgc
      181 ttctcaaaac ggagttaacg gctttcctcg attacgaaaa gtacgatcgc attggtttta
      241 acacgggtaa ttctcgtaac ggctcctatg accgtacggt caagaccgag tacggggaac
      301 ttcatctcca gattccgcgc gaccgcaacg gcgagttcaa gcaacagact gttcctgctt
      361 atagacggac gaatgatacc ttagaggaga ccgtcattca cctcttccga aaaggtatta
      421 ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg gcatcactac acgccccaaa
      481 ccatgtccaa catgactaaa gtgctgactg aagaagtaaa tgcatttaaa gttagaactc
      541 taaatgataa gtatgtcgct attttcatgg atgctactta tattccttta aaacggcaaa
      601 ccgtctccaa agaagcaatc tatattgcca tt
//
LOCUS       DACEMK010000243          562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEMK010000243 DACEMK010000000
VERSION     DACEMK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.253"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(39..124)
                     /locus_tag="IW715_002761"
     tRNA            complement(39..124)
                     /locus_tag="IW715_002761"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88..90),aa:Leu,seq:taa)
     gene            complement(141..212)
                     /locus_tag="IW715_002762"
     tRNA            complement(141..212)
                     /locus_tag="IW715_002762"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Gly,seq:gcc)
     gene            complement(224..296)
                     /locus_tag="IW715_002763"
     tRNA            complement(224..296)
                     /locus_tag="IW715_002763"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Thr,seq:tgt)
     gene            complement(314..395)
                     /locus_tag="IW715_002764"
     tRNA            complement(314..395)
                     /locus_tag="IW715_002764"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(359..361),aa:Leu,seq:tag)
     gene            complement(403..475)
                     /locus_tag="IW715_002765"
     tRNA            complement(403..475)
                     /locus_tag="IW715_002765"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Lys,seq:ttt)
     gene            complement(479..551)
                     /locus_tag="IW715_002766"
     tRNA            complement(479..551)
                     /locus_tag="IW715_002766"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Val,seq:tac)
BASE COUNT          143 a          160 c          135 g          124 t
ORIGIN      
        1 acactctatc cagttgagct atgggcgcat atttgaaatg ccgaggaccg gaatcgaacc
       61 ggtacggtga tcactcaccg caggatttta agtcctgtgc gtctgccagt tccgccaccc
      121 cggcgcgatt gctttggcaa agcggaaaac ggggttcgaa cccgcgaccc ccaccttggc
      181 aaggtggtgc tctaccactg agctatttcc gcgtgtttaa agatgccggc taaaggactt
      241 gaaccctcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggcatgt
      301 aaaacttaaa ttatgcgggt gaagggactt gaacccccac gccgtaaggc gctagatcct
      361 aaatctagtg cgtctgccaa ttccgccaca cccgcaaaaa tatgagccgt acagggctcg
      421 aacctgtgac cctctgatta aaagtcagat gctctaccaa ctgagctaac ggctcatatg
      481 gaggttaacg ggatcgaacc gctgaccctc tgcttgtaag gcagatgctc tcccagctga
      541 gctaaacctc catggataac tt
//
LOCUS       DACEMK010000244          547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.254, whole genome shotgun sequence.
ACCESSION   DACEMK010000244 DACEMK010000000
VERSION     DACEMK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.254"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(264..527)
                     /locus_tag="IW715_002767"
     CDS             complement(264..527)
                     /locus_tag="IW715_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ1023318.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          213 a           93 c           75 g          166 t
ORIGIN      
        1 tgaatttttt catgttgaaa acatcactaa tcaaaggcaa attataattt agaaatagaa
       61 tgaaaacact aaatgaaaat agaacattag aaaaaataga atcaaaaaaa cccaagacta
      121 gtgccgaaat aagggaaaca atcgaagcta caaaaaataa tgatttcgtt gaacaggacc
      181 aatttatttg tttccgatat ttctcaaaaa gaactttctt tgggaaagtt aaaggcgact
      241 gtaaagtcgc ctttagtcct tttttaccaa gctgctgcat atttagctcc atatttttta
      301 attaatgatt tggctgtagc aacaatacca gctgtaatta atccgcctcc aacaactgca
      361 gcaacaatag aaatgattgt agcaaccgtg ctaccagtat taattatgtc aataactttt
      421 ttagctgcat aagaagaaat tccaatgtta gcacttagca ttggtacacc aacaaagaat
      481 cccgcaaaac ctaaaattgg taaaactaat aataaattct ttttcataat aattcctcta
      541 ttttttt
//
LOCUS       DACEMK010000245          544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.255, whole genome shotgun sequence.
ACCESSION   DACEMK010000245 DACEMK010000000
VERSION     DACEMK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.255"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..285
                     /locus_tag="IW715_002768"
     CDS             <1..285
                     /locus_tag="IW715_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1023319.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          161 a           95 c          121 g          167 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaagatcaag ccttgaatga gataaattct
      421 tgctcgaaag ttatggaaat ttcgatagcc gtaggccact tgtattgtgc aatagttttt
      481 tacaaaaaaa agggggtgcg gatatttttt tggacctttt taacaaatat atctttgctt
      541 cttc
//
LOCUS       DACEMK010000246          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.denovo.256, whole genome shotgun sequence.
ACCESSION   DACEMK010000246 DACEMK010000000
VERSION     DACEMK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.denovo.256"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..424
                     /locus_tag="IW715_002769"
     CDS             128..424
                     /locus_tag="IW715_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1023320.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          207 a           74 c           86 g          150 t
ORIGIN      
        1 aatactataa aaaataaaaa acctttaaca actattggaa aaagaattac tttttgttca
       61 cggattatta acaactcttc ggtagagtaa acttatcaaa taaaaagact tctttggagg
      121 aatcactatg aaggaatata taaaaaacat ctattttatc gaagaaacac aaaatatcga
      181 gggttcttat attgaagtaa aaacactctt tgtcaatgaa gataaaacca aagcattgga
      241 tatttataaa aaattagctt caaaaaaaac gaatagtttt ggattgatat taagcgagta
      301 taaaatcaaa gcagaagaga gttatttcta tcaattatta aaacggtgga gtaagcttcc
      361 cgccgatttt tatcgaaaaa tgcagatcat caactatcaa ccgcttgctg agacacatgc
      421 ctaaatagag gttgtgaaat cagtgctttg agctaatacc gaaggtcagc tgatggaaca
      481 tcgtttagaa gagattgtga caactacgat ttgcttt
//
LOCUS       DACEMK010000247        20863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.047, whole genome shotgun sequence.
ACCESSION   DACEMK010000247 DACEMK010000000
VERSION     DACEMK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.047"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            267..1304
                     /locus_tag="IW715_002770"
     CDS             267..1304
                     /locus_tag="IW715_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1023321.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IW715_002771"
     CDS             complement(1393..1716)
                     /locus_tag="IW715_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1023322.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IW715_002772"
     CDS             complement(1703..2410)
                     /locus_tag="IW715_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1023323.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IW715_002773"
     CDS             2496..2837
                     /locus_tag="IW715_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1023324.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IW715_002774"
     CDS             complement(3008..3856)
                     /locus_tag="IW715_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023325.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IW715_002775"
     CDS             4454..5317
                     /locus_tag="IW715_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1023326.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IW715_002776"
     CDS             5417..5899
                     /locus_tag="IW715_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1023327.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IW715_002777"
     CDS             complement(6162..6629)
                     /locus_tag="IW715_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1023328.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IW715_002778"
     CDS             6752..7603
                     /locus_tag="IW715_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1023329.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IW715_002779"
     CDS             complement(7854..9812)
                     /locus_tag="IW715_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1023330.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IW715_002780"
     CDS             complement(9809..10681)
                     /locus_tag="IW715_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1023331.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IW715_002781"
     CDS             complement(11023..11895)
                     /locus_tag="IW715_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1023332.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IW715_002782"
     CDS             12023..12592
                     /locus_tag="IW715_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1023333.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IW715_002783"
     CDS             12659..13396
                     /locus_tag="IW715_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1023334.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IW715_002784"
     CDS             13627..14895
                     /locus_tag="IW715_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1023335.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IW715_002785"
     CDS             15382..15846
                     /locus_tag="IW715_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1023336.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IW715_002786"
     CDS             15830..16459
                     /locus_tag="IW715_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1023337.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IW715_002787"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IW715_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1023338.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IW715_002788"
     CDS             18338..18784
                     /locus_tag="IW715_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1023339.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IW715_002789"
     CDS             complement(18983..19498)
                     /locus_tag="IW715_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1023340.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20817
                     /locus_tag="IW715_002790"
     CDS             19615..20817
                     /locus_tag="IW715_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1023341.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         6632 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
    19921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
    19981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
    20041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
    20101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
    20161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
    20221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
    20281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
    20341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
    20401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
    20461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
    20521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
    20581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
    20641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
    20701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
    20761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
    20821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DACEMK010000248        16928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.058, whole genome shotgun sequence.
ACCESSION   DACEMK010000248 DACEMK010000000
VERSION     DACEMK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.058"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(134..577)
                     /locus_tag="IW715_002791"
     CDS             complement(134..577)
                     /locus_tag="IW715_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1023342.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IW715_002792"
     CDS             complement(795..2135)
                     /locus_tag="IW715_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1023343.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IW715_002793"
     CDS             2404..3300
                     /locus_tag="IW715_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1023344.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IW715_002794"
     CDS             complement(3450..4391)
                     /locus_tag="IW715_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1023345.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IW715_002795"
     CDS             4517..5638
                     /locus_tag="IW715_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1023346.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IW715_002796"
     CDS             5896..7398
                     /locus_tag="IW715_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1023347.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IW715_002797"
     CDS             7465..7992
                     /locus_tag="IW715_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1023348.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IW715_002798"
     CDS             8203..9264
                     /locus_tag="IW715_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1023349.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IW715_002799"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IW715_002799"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1023350.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IW715_002800"
     CDS             10177..12159
                     /locus_tag="IW715_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1023351.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW715_002801"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW715_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1023352.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IW715_002802"
     CDS             12881..14305
                     /locus_tag="IW715_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1023353.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IW715_002803"
     CDS             complement(14486..15259)
                     /locus_tag="IW715_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1023354.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IW715_002804"
     CDS             complement(15256..16161)
                     /locus_tag="IW715_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1023355.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IW715_002805"
     CDS             complement(16315..16635)
                     /locus_tag="IW715_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1023356.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5462 a         3092 c         3475 g         4899 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttcta
//
LOCUS       DACEMK010000249        11422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.090, whole genome shotgun sequence.
ACCESSION   DACEMK010000249 DACEMK010000000
VERSION     DACEMK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.090"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            39..977
                     /locus_tag="IW715_002806"
                     /pseudo
     CDS             39..977
                     /locus_tag="IW715_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IW715_002807"
     CDS             980..3163
                     /locus_tag="IW715_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1023357.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IW715_002808"
     CDS             3160..4161
                     /locus_tag="IW715_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1023358.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IW715_002809"
     CDS             4176..5090
                     /locus_tag="IW715_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1023359.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IW715_002810"
     CDS             5335..5451
                     /locus_tag="IW715_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1023360.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW715_002811"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW715_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1023361.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW715_002812"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW715_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1023362.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IW715_002813"
     CDS             9421..10683
                     /locus_tag="IW715_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1023363.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IW715_002814"
     CDS             10928..11410
                     /locus_tag="IW715_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1023364.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3771 a         1907 c         2388 g         3356 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aa
//
LOCUS       DACEMK010000250         8035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.110, whole genome shotgun sequence.
ACCESSION   DACEMK010000250 DACEMK010000000
VERSION     DACEMK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.110"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            289..564
                     /locus_tag="IW715_002815"
     CDS             289..564
                     /locus_tag="IW715_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1023365.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IW715_002816"
     CDS             577..2655
                     /locus_tag="IW715_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1023366.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IW715_002817"
     CDS             2815..4701
                     /locus_tag="IW715_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1023367.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IW715_002818"
     CDS             4713..5660
                     /locus_tag="IW715_002818"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1023368.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6207
                     /locus_tag="IW715_002819"
     CDS             5680..6207
                     /locus_tag="IW715_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1023369.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6270..6923)
                     /locus_tag="IW715_002820"
     CDS             complement(6270..6923)
                     /locus_tag="IW715_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1023370.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7055..7897
                     /locus_tag="IW715_002821"
     CDS             7055..7897
                     /locus_tag="IW715_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1023371.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
BASE COUNT         2740 a         1351 c         1695 g         2249 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
     5821 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
     5881 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
     5941 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
     6001 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
     6061 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
     6121 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
     6181 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
     6241 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
     6301 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
     6361 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
     6421 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
     6481 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
     6541 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
     6601 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
     6661 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
     6721 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
     6781 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
     6841 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
     6901 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
     6961 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
     7021 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
     7081 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
     7141 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
     7201 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
     7261 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
     7321 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
     7381 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
     7441 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
     7501 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
     7561 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
     7621 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
     7681 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
     7741 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
     7801 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
     7861 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
     7921 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
     7981 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatc
//
LOCUS       DACEMK010000251         7959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.111, whole genome shotgun sequence.
ACCESSION   DACEMK010000251 DACEMK010000000
VERSION     DACEMK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.111"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW715_002822"
     CDS             128..1564
                     /locus_tag="IW715_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1023372.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW715_002823"
     CDS             1753..4200
                     /locus_tag="IW715_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1023373.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW715_002824"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW715_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1023374.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW715_002825"
     CDS             complement(5690..6793)
                     /locus_tag="IW715_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1023375.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW715_002826"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW715_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1023376.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACEMK010000252         4472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.137, whole genome shotgun sequence.
ACCESSION   DACEMK010000252 DACEMK010000000
VERSION     DACEMK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.137"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            270..1484
                     /locus_tag="IW715_002827"
     CDS             270..1484
                     /locus_tag="IW715_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1023377.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1515..1733
                     /locus_tag="IW715_002828"
                     /pseudo
     CDS             1515..1733
                     /locus_tag="IW715_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2152..2625
                     /gene="dfrF"
                     /locus_tag="IW715_002829"
     CDS             2152..2625
                     /gene="dfrF"
                     /locus_tag="IW715_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1023378.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2752..3408
                     /locus_tag="IW715_002830"
     CDS             2752..3408
                     /locus_tag="IW715_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1023379.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3590..4120
                     /locus_tag="IW715_002831"
     CDS             3590..4120
                     /locus_tag="IW715_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1023380.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1495 a          654 c          945 g         1378 t
ORIGIN      
        1 ttttttatat attttatatg aagtattctt tattccttat gaaaaaatat ctttaaaaat
       61 aaaaaaagac gaaaataaac ggttttctat aaaaagaaga ccgtttattt tttgtcttta
      121 tttgtgtgaa aaacgaaaaa gagcacaaca catcaagctt ttattaattg tctgtctttt
      181 ataaatgcaa gtattaattc aaaaaaacag aagaaaaaaa caactacacc ttcttttata
      241 ctcgtaatcg tagaaaggag gcgaaaaaaa tgaatacatt acctatcaat acaggagata
      301 cagcattcat gattttatgt acagcaatgg tttgcctgat gactcctggt ctagcttttt
      361 tttacggcgg attagcacgg aagaataata tactaacgat catgtctcaa tcttttctct
      421 ccgttgggat aacaacgatt atgtgggtat ttggtggatt cggattggcc tttggacggg
      481 atatcggcgg tgttattgga aacccggcag atttcttttt gatgagacat gttacaagct
      541 tccccaacat gtttcatggt gcaacgattc catttttaat gttctttctt taccaactga
      601 tgtttatcgt gattaccgtt cctttgatga caggagcttt tgcagggaga ttgaatttaa
      661 aaggctatat tatacttgtt attttttgga atctgctgat ttatttccct gtttgccact
      721 ggatatgggg tggcggattt ttggatcaga tgggatttag agatttcgct ggtggagcag
      781 tcatccacac gacagctgga ttcggctctt tagcatgtat cctcacactt ggtcaaagaa
      841 agattccttt aggagaaaga agtcagcata gcagtcttat ggcagctgct atcggtacag
      901 gactgctttg gtttggctgg ttcggtttta attccggtgg tgcacttcga gcagatgaac
      961 aagcagtcaa tgcatttggt tcaacattca tcgctcttgc atttgcgatg attacttggc
     1021 tgatcattgc aaaagtaaaa ggaaatggct ttgattttgt cgacgtacta acaggatccg
     1081 ttgctggatt ggcagccatt acgccttgtg ccggttatgt agaagcaaaa agtgccatgc
     1141 tgattgggat cattgcgggt atcgtctgtc atgctgctgt tgattttcgt aaaaagaaac
     1201 aatgggatga tgcacttgat gtgtggggtg ttcatggcat gggcggattc acaggtacga
     1261 ttctgatagg gatctttgca tctaacagcc atttactaac gaatccaagc agctggtact
     1321 tcttagggat tcaagtgatt ggtgtcatca ttactgccgt gtatgcctat gtgctgacaa
     1381 cagtgatttt aaaatcggcg atgcacttta caacgatcac tactacaaaa gaagaacaag
     1441 aagaaggatt ggactttttc ttctttggca acaaactcaa ttgaaacaaa ttcaaataga
     1501 aaagaggaag attcatgcaa caaagcaaaa cgaaaattcc tgcaaaaaaa ttaacgttct
     1561 ttggcttttt ggctatgacc atctcaatgg tcgtttcatt atatgaatat cccacattcg
     1621 cgacctcagg cttcagctta gtatttttct tgttattagg cggtttgcta tggtttatcc
     1681 ctgtggcatt gtgcgcagca gagatggcaa cggtgaaagg atgggaaaaa ggtgtttccc
     1741 aaacaaaaag atattaagaa acagcccatg tttacctgct tgcaaacaaa agaatattgc
     1801 tactagacta agagatacta cataagtgtg ttggtatctc ttttttgttg ccgtgaaaca
     1861 gcagactatt tcatttctgc ttgattacct taaaacttat tagtctatgg tttgtcaagg
     1921 gcttgttgcg tgagaaatgc tgaatatcac cctttacaaa acataggctg ataagtaact
     1981 tttcaaaagc agaaaagaaa tggcaatgct tataacaata tgatataatg cagatgaaga
     2041 caaaaaagga atttgtcgag ctggttagtt cgagataatt ttgagtttgt taagatgtgg
     2101 tgcacttgat ggaatcacag gtacgacatc atattatgga ggatgaaata gatgataggt
     2161 ttgattgttg cgaggtcaaa gaataatgtt ataggcaaga atggtaatat accatggaaa
     2221 ataaagggag aacaaaagca atttagagag ttaacaacgg gtaatgtggt tattatgggg
     2281 cgaaagtctt atgaagaaat cggtcatccg ttgcctaata gaatgaatat tgttgtttcc
     2341 accacaacag agtatcaagg agataattta gtttcagtta aatcattaga agatgcatta
     2401 ttattggcta aaggacgaga tgtatacata tctggtggat atggactatt taaggaagct
     2461 ttgcaaatag tagataaaat gtatatcaca gaagtagatt taaatattga agatggagat
     2521 acattctttc cagaatttga tatcaatgat tttgaagttt tgatagggga aacacttggt
     2581 gaggaagtga aatatacgag aacattttat gtaaggaaaa aatgaattga gtaaattttg
     2641 gatttagggt gttttcataa atatattgct ttttatgcat atataaattg ttgtaagaca
     2701 aattccagtt tgtatggggc gataaaatca gaatttgaga gaggattgaa tatgaataag
     2761 gaatggtctg aacttaataa aacaatgcag gcacaaataa aaaagaaaga tacttataag
     2821 aggggtattg atactttact tactttacga agtcagttaa tacaaacctt agtatctttc
     2881 aaagaagaat tatgcagaga agattttaac tcaatcccct tcataaacgc tgatggttat
     2941 catagtaaga cgattgctta ttctatttgg catattttcc gcattgaaga tatagttgtg
     3001 catacagtga taaatgaaga tgaacaagta ttttttgcag gcaattatca agagcgtatc
     3061 aattcaccta tcattacaac aggaaatgaa ctcatgaaac agcagattgc agacttttca
     3121 aaacagctta atctggaaga attatattta tatattttcg aagtatggga aagcactgag
     3181 aaaatgcttg aacggttatc ttatgatgaa ttgaaaagaa aaataccgaa agaaagaaaa
     3241 ggatacttag aatcgttgaa tgtagtaaac gacaatgaaa aagcaatttg gctgattgat
     3301 tattggtgta ataaagatat ttgtggacta attcaaatgc cgttttcaag acattggatt
     3361 atgcacacag aagcctgttt gcgtataaag aacaagatac attcataggc aaaaatttca
     3421 gtttgtagaa ctaaggaaat tggaatttaa ggaggaataa agtatggtta gtgattttat
     3481 aagagcagca tttccatgga ttttaatggg tttatttgta gcaatcagtt gttcccttat
     3541 gagtaaaaaa gagaaataaa ttcctgtttg tatttataaa agagaaaaaa tgagaaaagt
     3601 agttttattt attgccatga gccttgacgg atatattgcg gacggtaatg gtggagtagc
     3661 ttggctaaat ggtcatggaa atgacaatga aaacatcgac acttatacag aatttaccaa
     3721 agatatagat actgtcttaa tggggtggaa tacttctcat caggttgtta ctgaactttc
     3781 tccgcaagaa tgggtatata ataaatttac aacttatgta ttaacacata aagagtgcaa
     3841 ttcttcccaa caaattcatt ttacaagtga aaatcctgtt ttattattgg aacggctaaa
     3901 acaagaagca ggaaaggata tttggatttg tggcggagca aatcttgttc agcagttggt
     3961 aagcaaaaat atgattgata aatattacat ttctgttatt cctactctgt taggaaatgg
     4021 ggttcgtctt ttgggcaaca tagacaggga aataaagtta aggctttgta aaacacagac
     4081 ttataacgga ataaccgatt tgatttatat gcgtagataa ctagagagaa acagaaaagc
     4141 tgttaaaccg acggagcgat ccgaccacgt tggcgggaaa atccttaaga tttttccgcc
     4201 atgcttgcaa gggggcggtt cgcttcggcg gttcggcgag cttgcgagcc gttcaaagcc
     4261 cccgttatct aacaaggggg gcacaaaaaa caagaaacac tatacactaa caagcggaaa
     4321 acgctgtatt tataaggcaa aaccccattt ttacagtagt gatataaatt gtacctatat
     4381 cactatgaaa attgaatagt aaatatgaga aagacagttg atttcaaaaa ataggctctt
     4441 tgtcaataag gactgatggg ttgtacaaaa tc
//
LOCUS       DACEMK010000253         3278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.169, whole genome shotgun sequence.
ACCESSION   DACEMK010000253 DACEMK010000000
VERSION     DACEMK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.169"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            245..1213
                     /locus_tag="IW715_002832"
     CDS             245..1213
                     /locus_tag="IW715_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1023381.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IW715_002833"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IW715_002833"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1023382.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IW715_002834"
     CDS             2243..2851
                     /locus_tag="IW715_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1023383.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          980 a          626 c          792 g          880 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaa
//
LOCUS       DACEMK010000254         2553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.184, whole genome shotgun sequence.
ACCESSION   DACEMK010000254 DACEMK010000000
VERSION     DACEMK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.184"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..459
                     /locus_tag="IW715_002835"
     CDS             <1..459
                     /locus_tag="IW715_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1023384.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR"
                     /locus_tag="IW715_002836"
     CDS             673..1368
                     /gene="vanR"
                     /locus_tag="IW715_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1023385.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="IW715_002837"
     CDS             1346..2500
                     /locus_tag="IW715_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1023386.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          880 a          454 c          535 g          684 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa tct
//
LOCUS       DACEMK010000255         2439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.187, whole genome shotgun sequence.
ACCESSION   DACEMK010000255 DACEMK010000000
VERSION     DACEMK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.187"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            9..920
                     /locus_tag="IW715_002838"
     CDS             9..920
                     /locus_tag="IW715_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1023387.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1073..1558
                     /gene="vanZ"
                     /locus_tag="IW715_002839"
     CDS             1073..1558
                     /gene="vanZ"
                     /locus_tag="IW715_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1023388.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1845..2174)
                     /locus_tag="IW715_002840"
     CDS             complement(1845..2174)
                     /locus_tag="IW715_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1023389.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
BASE COUNT          849 a          358 c          421 g          811 t
ORIGIN      
        1 gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata tacttaggtt
       61 atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa aattatgatc
      121 aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa gagaaaacaa
      181 ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa tatcctgttc
      241 gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa ttaataaatg
      301 gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa aaattttcag
      361 agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat agtggctatc
      421 gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat gccttaccag
      481 caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc ttgacgaaaa
      541 tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac gggttcattt
      601 tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca tggcatattc
      661 gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt ctcgaggaat
      721 atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg gaaaaatatg
      781 agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact aatcttcgtt
      841 atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc ggatcaacac
      901 atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat gaacagcatt
      961 attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac gttaactagg
     1021 acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta tattgggaaa
     1081 aatattatct agaggattgc tagctttata tttagtgaca ctaatctggt tagtgttatt
     1141 caaattacaa tacaatattt tatcagtatt taattatcat caaagaagtc ttaacttgac
     1201 tccatttact gctactggga atttcagaga gatgatagat aatgttataa tctttattcc
     1261 atttggcttg cttttgaatg tcaattttaa agaaatcgga tttttaccta agtttgcttt
     1321 tgtactggtt ttaagtctta cttttgaaat aattcaattt atcttcgcta ttggagcgac
     1381 agacataaca gatgtaatta caaatactgt tggaggcttt cttggactga aattatatgg
     1441 tttaagcaat aagcatatga atcaaaaaaa attagacaga gttattattt ttgtaggtat
     1501 acttttgctc gtattattgc tcgtttaccg tacccattta agaataaatt acgtgtaaga
     1561 tgtctaaatc aagcaatctg atctttcata cacataaaga tattgaatga attggattag
     1621 atggaaaacg ggatgtgggg aaactcgccc gtaggtgtga agtgagggga aaaccggtga
     1681 taaagtaaaa agcttaccta acactatagt aacaaagaaa gcccaattat caattttagt
     1741 gctgaggaat tggtctcttt aataaatttc cttaacgttg taaatccgca ttttcctgac
     1801 ggtaccccca aactaagatt tcatagatag attttctgat aactttaaaa aacacgaata
     1861 gcggaaagga tgtcttcatt cgctaaagag taataaacta cttttccatc ttgacgtgat
     1921 tttgccattc catgtttttt taaaaaacgt aaatggtgtg acgttgtagc gacactagca
     1981 gtaagtattt ctgctaaatc acagacgcac atttccttat aggtttctaa agcatagaaa
     2041 attttaattc tagaaaaatc gctgaaacat ttccctaaca caagtaagtt tgaaaaatct
     2101 ttttgatcca atttattttt tattccattt actttttctt tatgtacgac agtcacttgg
     2161 caaacttttt tcattcatct cacctcacat tcaaacgatt atttgaatga agtataccta
     2221 tttgatcact cttttgtcaa tctaccttaa ttccaataca ctattataag caatttcatg
     2281 tattgaagta aacttacttg caagtgcgtt tttgattcca agttaaaaac atattattat
     2341 tctacataat ttcctaatca aataaaactc ctttgaaaat tgttctaaag gagttttatt
     2401 attttgtatt tctactaaaa atagcgtaca atgtaatta
//
LOCUS       DACEMK010000256         1650 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E428
            SAMN07141489-rid9868853.guided.206, whole genome shotgun sequence.
ACCESSION   DACEMK010000256 DACEMK010000000
VERSION     DACEMK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141489
            Sequence Read Archive: SRR5811463
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740793.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740793.1
            Genome Coverage       :: 56x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 00:35:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,755
            CDSs (with protein)               :: 2,755
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 28 of 61
            Pseudo Genes (incomplete)         :: 26 of 61
            Pseudo Genes (internal stop)      :: 18 of 61
            Pseudo Genes (multiple problems)  :: 10 of 61
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141489-rid9868853.guided.206"
                     /isolate="E428"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="Jan-2009"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1348
                     /locus_tag="IW715_002841"
     CDS             <1..1348
                     /locus_tag="IW715_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ1023390.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1478..>1650)
                     /locus_tag="IW715_002842"
     CDS             complement(1478..>1650)
                     /locus_tag="IW715_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1023391.1"
                     /translation="KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEW
                     IYSSRKYINFDK"
BASE COUNT          671 a          148 c          278 g          553 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
     1501 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
     1561 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
     1621 tcagtcgttc aattagattg gtactcttta
//
